scholarly journals A Study on Extracellular Synthesis of Silver Nanoparticles from Endophytic Fungi, Isolated from Ethanomedicinal Plants Curcuma longa and Catharanthus roseus

2016 ◽  
Vol 57 ◽  
pp. 58-66 ◽  
Author(s):  
Dattu Singh ◽  
Vandana Rathod ◽  
Ashish Kumar Singh ◽  
Manzoor Ul Haq ◽  
Jasmine Mathew ◽  
...  

Biological method is considered as eco-friendly and reliable process for the synthesis of silver nanoparticles (AgNps) in the field of nanotechnology due to its tremendous applications in various fields. In this study we have isolated a total of twelve endophytic fungi from leaves ofCurcumalonga(turmeric) andCatharanthusroseusout of which six endophytic fungi showed their ability to synthesized AgNps from silver nitrate (AgNO3)solution which splits into a positive silver ion (Ag+) and a negative nitrate ion (NO3-) in order to turn the silver ions into solid silver (Ago). Of the six positive endophytic fungi VRD2 showed good and encouraging results and was identified asPenicillium spinulosumVRD2. UV-Visible Spectroscopy confirms the AgNps showing maximum peak at 425nm implying the bioreduction of AgNO3. Transmission Electron Microscopy (TEM) revealed the particle are spherical and well dispersed without agglomeration size ranging from 25-30nm.

2011 ◽  
Vol 109 ◽  
pp. 174-177 ◽  
Author(s):  
Yu Li Shi ◽  
Qi Zhou ◽  
Li Yun Lv ◽  
Wang Hong

A facile method for the synthesis of silver nanoparticles (NPs) has been developed by using sodium phosphate (Na3PO4) as stabilizing agents and glucose the reducing agent, respectively. The obtained silver NPs have been characterized by X-ray diffraction (XRD), UV-visible spectroscopy (UV-vis) and transmission electron microscopy (TEM). It was found that in the presence of sodium phosphate, silver NPs with different morphologies and sizes were obtained. The formation mechanism of diverse silver NPs was studied preliminarily.


Author(s):  
Vidyasagar G M ◽  
Shankaravva B ◽  
R Begum ◽  
Imrose ◽  
Sagar R ◽  
...  

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.


Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5798
Author(s):  
Amir Reza Sadrolhosseini ◽  
Ganesan Krishnan ◽  
Suhaidi Shafie ◽  
Suraya Abdul Rashid ◽  
Sulaiman Wadi Harun

This study used the carbon dots solution for the laser ablation technique to fabricate silver nanoparticles. The ablation time range was from 5 min to 20 min. Analytical methods, including Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy, transmission electron microscopy, and Raman spectroscopy were used to categorize the prepared samples. The UV-visible and z-scan techniques provided optical parameters such as linear and nonlinear refractive indices in the range of 1.56759 to 1.81288 and 7.3769 × 10−10 cm2 W−1 to 9.5269 × 10−10 cm2 W−1 and the nonlinear susceptibility was measured in the range of 5.46 × 10−8 to 6.97 × 10−8 esu. The thermal effusivity of prepared samples, which were measured using the photoacoustic technique, were in the range of 0.0941 W s1/2 cm−2 K−1 to 0.8491 W s1/2 cm−2 K−1. The interaction of the prepared sample with fluoride was investigated using a Raman spectrometer. Consequently, the intensity of the Raman signal decreased with the increasing concentration of fluoride, and the detection limit is about 0.1 ppm.


2013 ◽  
Vol 873 ◽  
pp. 206-210
Author(s):  
Kai Li ◽  
Rao Fu ◽  
Qing Ran Gao ◽  
Ai Wei Tang ◽  
Ying Feng Wang

This paper continues our previous work on preparation of triangular silver nanoparticles. The method proceeds with reaction of silver nitrate with hydrazine hydrate in the presence of polyvinyl pyrrolidone in aqueous solution. Effects of the concentration of PVP on the morphologies of Ag NPs were systematically investigated. The obtained Ag NPs were characterized by transmission electron microscopy and UV-visible spectrophotometer. The results showed that, triangular Ag NPs with edge lengths in the range of 50-200 nm were obtained using PVP as protective agent with lower concentration. As the concentration of PVP increased, spherical Ag NPs with their sizes about 6.2 nm were prepared and triangular Ag NPs were not obtained. The formation mechanism of triangular Ag NPs has been studied. Ostwald ripening is the driving force on the conversion of spherical Ag NPs to triangular Ag NPs in the presence of PVP.


Author(s):  
Shyla Marjorie Haqq ◽  
Amit Chattree

  This review is based on the synthesis of silver nanoparticles (AgNPs) using a green approach which is biofabricated from various medicinal plants. AgNPs were prepared from the various parts of the plants such as the flowers, stems, leaves, and fruits. Various physiochemical characterizations were performed using the ultraviolet (UV)-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction spectroscopy, transmission electron microscopy, and energy dispersive spectroscopy. AgNPs were also used to inhibit the growth of bacterial pathogens and were found to be effective against both the Gram-positive and Gram-negative bacteria. For the silver to have antimicrobial properties, it must be present in the ionized form. All the forms of silver-containing compounds with the observed antimicrobial properties are in one way or another source of silver ions. Although the antimicrobial properties of silver have been known, it is thought that the silver atoms bind to the thiol groups in enzymes and subsequently leads to the deactivation of enzymes. For the silver to have antimicrobial properties, it must be present in the ionized form. The study suggested that the action of the AgNPs on the microbial cells resulted into cell lysis and DNA damage. AgNPs have proved their candidature as a potential antibacterial against the multidrug-resistant microbes. The biological agents for synthesizing AgNPs cover compounds produced naturally in microbes and plants. Reaction parameters under which the AgNPs were being synthesized hold prominent impact on their size, shape, and application. Silver nanoparticle synthesis and their application are summarized and critically discussed in this review.


Author(s):  
Robert Lotha ◽  
Aravind Sivasubramanian ◽  
Meenakshi Sundaram Muthuraman

Objective: The present study was aimed at the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Euphorbia cyathophora leavesand testing their anticancer potential using HT-29 cell line model.Methods: Green synthesis of silver nanoparticles was obtained with the aqueous extract of E. cyathophora. The synthesized nanoparticles wereconfirmed initially by ultraviolet-visible spectroscopy. Further, scanning electron microscopy, transmission electron microscopy, and X-Ray diffractionstudies also ensured the presence of silver nanoparticles. Zeta potential studies revealed the stability of the silver nanoparticles.Results: Antioxidant and anticancer studies of the nanoparticles against HT-29 cell line exhibited remarkable results.Conclusion: This ensures that the synthesized nanoparticles play an important role in medicinal biology.


2012 ◽  
Vol 585 ◽  
pp. 144-148
Author(s):  
Poushpi Dwivedi ◽  
S.S. Narvi ◽  
R.P. Tewari

In this nanoregime attempts to bring forth nanoparticles and nanomaterials are myriads, with there interesting and demanding applications in almost every field. Today the field of nanoscience has bloomed with the confluence of nanotechnology with material science, biology, biotechnology and medicine and the need for nanotechnology will only increase as miniaturization becomes extremely important in various arrays of life. Since time immemorial silver nanoparticles have been extensively used for hygienic and healing purposes, and even until most recently, it has indispensible vital role especially in the biomedical arena. Thus in an attempt to generate silver nanoparticles employing green, environmentally benign route, we have designed to converge mythology with technology, with the mystical production of silver nanoparticles, enabled by the blueberry beads of the plant Elaeocarpus granitrus Roxb., the Rudraksha. This non-degradable bead does not disintegrate, but retains the potentiality, even after unlimited production of silver nanoparticles, assisting infinite times. The extremely cost-efficient nanoparticles thus developed in a superiorly efficient manner were characterized through different techniques; like UV/visible spectroscopy, PL spectroscopy, transmission electron microscopy, energy dispersive X-ray analysis and nanoparticle size analysis.


2016 ◽  
Vol 5 (6) ◽  
Author(s):  
Brajesh Kumar ◽  
Kumari Smita ◽  
Luis Cumbal

AbstractThe present report summarizes an eco-friendly approach for the biosynthesis of silver nanoparticles (AgNPs) using the leaf extract of lavender. Initially, the synthesis of AgNPs was visually observed by the appearance of a wine red color. The optical property, morphology, and structure of as-synthesized AgNPs were characterized by UV-visible spectroscopy, dynamic light scattering, transmission electron microscopy, and X-ray diffraction analyses. All characterization data revealed the formation of crystalline and spherical AgNPs (Ag/Ag


2011 ◽  
Vol 356-360 ◽  
pp. 524-528 ◽  
Author(s):  
Chun Ling Yu ◽  
Rui Xue Wu ◽  
Ying Huan Fu ◽  
Xiao Li Dong ◽  
Hong Chao Ma

A polyaniline supported titanium dioxide photocatalyst was prepared by an impregnation-hydrothermal process and characterized by powder X-ray diffraction, transmission electron microscopy and UV-visible spectroscopy. It was found that the TiO2 nanoparticles were well dispersed on the surface of the polyaniline and the photocatalyst has a stronger absorption compared with that of pure TiO2 over the whole of the visible spectrum. The photocatalyst exhibited higher photocatalytic activity than pure TiO2 for the photodegradation of solutions of the anthraquinone dye, reactive brilliant blue KN-R, under visible light irradiation.


2016 ◽  
Vol 32 (1-2) ◽  
pp. 45
Author(s):  
Swarnali Maiti ◽  
Gadadhar Barman ◽  
Jayasree Konar Laha

Interaction of 3 -mercapto-1, 2- propanediol (3MPD) (C<sub>3</sub>H<sub>8</sub>O<sub>2</sub>S, 3MPD) with silver nanoparticles prepared by green method has been studied by electronic absorption spectroscopy and transmission electron microscopy. Here we report the changes in properties of AgNP in presence of biologically relevant molecule 3MPD. The assembly and the aggregation of the AgNP are established using UV-Visible spectra and transmission electron microscopy. The biosynthesized AgNP has been used as colorimetric sensor for detection of 3MPD. A new peak generated at 612 nm due to the self aggregation of silver nanoparticles occurred by the interaction of thiol group present in 3 MPD with AgNP. A calibration curve between the absorbance at 612 nm and the concentration of 3 MPD enabled us to estimate 3MPD present in water in presence of SDS.


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