Ischemia modified albumin as an early biomarker in diagnosing the late stages abortus in cows

2017 ◽  
Author(s):  
Beran Yokus ◽  
Nazli Ercan ◽  
Ulvi Fidancý ◽  
Mustafa Gün
Keyword(s):  
Oxidative Stress ◽  
Positive Control ◽  
Maternal Serum ◽  
Stress Index ◽  
Control Group ◽  
Serum Samples ◽  
Ischemia Modified Albumin ◽  
Early Biomarker ◽  
Total Oxidative Stress ◽  
Complicated Pregnancy

Nowadays, Ischemia Modified Albumin (IMA) has been regarded as an indicator for various maladies and complicated pregnancy in humans. Therefore, it is hypothesized that evaluation of the maternal serum IMA degrees can serve as an early detection of abortion in dairy cows. 252 Holstein cows’ serum samples were taken both in the 2nd and 6th months of their pregnancy. Twenty-two cattle were selected as a positive control group among the non aborted ones. Furthermore, blood samples from 20 non-pregnant cows were taken to identify the effects of physiological changes of pregnancy. IMA and Ischemia Modified Albumin Rate (IMAR) were markedly higher (p less than 0.01); and albumin was markedly lower (p less than 0.01) in aborted cows and healthy-pregnant cows compared with non-pregnant cows in both months. In aborted cows, IMA, IMAR and Oxidative stress index (OSI) degrees were found to be more increased than healthy pregnancies both in 2nd and 6th months of pregnancy; however, there were statistically significant only at 6th month (p less than 0.01). Total Oxidative Stress (TOS) levels were higher than healthy pregnancies. So, as a result of IMA which was caused by oxidative stress rising from hypoxia/ischemia could serve as an early bioindicator for miscarriage in cattle.

scholarly journals The Role of Oxidative Stress in the Etiopathogenesis of Gluten-Sensitive Enteropathy Disease

2017 ◽  
Vol 36 (3) ◽  
pp. 243-250 ◽  
Author(s):  
Mustafa Kaplan ◽  
Ihsan Ates ◽  
Mahmut Yüksel ◽  
Yasemin Ozderin Ozin ◽  
Muhammed Yener Akpinar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Paraoxonase 1 ◽  
Stress Index ◽  
Control Group ◽  
Gluten Free ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Reactive Protein ◽  
Gluten Sensitive Enteropathy

SummaryBackground:The objective here is to examine the role of overall oxidative stress in the etiopathogenesis of gluten-sensitive enteropathy disease and its relationship with gluten free diet and autoantibodies.Methods:Eighty gluten-sensitive enteropathy patients and 80 control group participants were included in the study. As oxidative stress parameters, we researched total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), paraoxonase-1 and arylesterase parameters in the serum samples of gluten-sensitive enteropathy patients.Results:In comparison to the control group, gluten-sensitive enteropathy patients had lower TAS, paraoxonase-1 and arylesterase levels and gluten-sensitive enteropathy patients had considerable TOS and OSI levels. In contrast, patients who agreed to the gluten free eating routine had a higher OSI proportion and patients who did not conform to the gluten free eating regimen had a lower paraoxonase-1 level. An affirming reciprocation was de tected amidst TOS and OSI proportion and gluten-sensitive enteropathy autoantibodies and C-reactive protein levels and a negative correlation was found between arylesterase level and gluten-sensitive enteropathy autoantibodies.Conclusions:We observed oxidative stress levels to be higher in gluten-sensitive enteropathy patients contrasted with the control group. Oxidative stress level showed differences in gluten-sensitive enteropathy patients depending on gluten diet content and autoantibody positivity. In point of fact, C-reactive protein and gluten-sensitive enteropathy autoantibodies are identified with oxidative anxiety parameters resulting in the possibility that oxidative stress might be successful in the gluten-sensitive enteropathy pathogenesis.

scholarly journals Clinical Significance of ROMs, OXY, SHp and HMGB-1 in Canine Leishmaniosis

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 754
Author(s):  
Michela Pugliese ◽  
Alessandra Sfacteria ◽  
Gaetano Oliva ◽  
Annastella Falcone ◽  
Manuela Gizzarelli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lymph Nodes ◽  
Clinical Signs ◽  
Biological Tissues ◽  
Stress Index ◽  
Control Group ◽  
Canine Leishmaniosis ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

This study aimed to investigate the role of oxidative stress parameters (ROMs, OXY, SHp), the Oxidative Stress index (OSi), and High Mobility Group Box-1 protein (HMGB-1) in canine leishmaniosis (CanL). For this study, thirty dogs, naturally infected with Leishmania spp. (Leishmania Group, LEISH) and ten healthy adult dogs (control group, CTR) were included. The diagnosis of CanL was performed by a cytological examination of lymph nodes, real time polymerase chain reaction on biological tissues (lymph nodes and whole blood), and an immunofluorescence antibody test (IFAT) for the detection of anti-Leishmania antibodies associated with clinical signs such as dermatitis, lymphadenopathy, onychogryphosis, weight loss, cachexia, lameness, conjunctivitis, epistaxis, and hepatosplenomegaly. The HMGB-1 and oxidative stress parameters of the LEISH Group were compared with the values recorded in the CTR group (Mann Whitney Test, p < 0.05). Spearman rank correlation was applied to evaluate the correlation between the HMGB-1, oxidative stress biomarkers, hematological and biochemical parameters in the LEISH Group. Results showed statistically significant higher values of SHp in the LEISH Group. Specific correlation between the ROMs and the number of red blood cells, and between HGMB-1 and SHp were recorded. These preliminary data may suggest the potential role of oxidative stress in the pathogenesis of CanL. Further studies are undoubtedly required to evaluate the direct correlation between inflammation parameters with the different stages of CanL. Similarly, further research should investigate the role of ROMs in the onset of anemia.

Evaluation of Dynamic Thiol-Disulfide Balance in Preinvasive Lesions of the Cervix

2021 ◽  
Author(s):  
Burak Sezgin ◽  
Fatih Pirinççi ◽  
Aysun Camuzcuoğlu ◽  
Eda Adeviye Şahin ◽  
Özcan Erel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Papillomavirus ◽  
Stress Index ◽  
Lesion Group ◽  
Control Group ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Oxidative Stress Index ◽  
Preinvasive Lesions ◽  
Intraepithelial Lesion

Abstract Purpose: This study aimed to determine the potential clinical use of dynamic thiol disulfide balance in cases with preinvasive lesions of the cervix.Methods: One hundred and sixteen patients with high-grade squamous intraepithelial lesion, one hundred patients with low-grade squamous intraepithelial lesion and one hundred and ten healthy controls were enrolled in the study. A fully automated colorimetric system was used to determine the levels of thiol-disulfide parameters. The ischemia-modified albumin, total oxidant-antioxidant capacity, oxidative stress index of the retrieved cases were further analysed.Results: Native thiol and total thiol levels are significantly lower in the high-grade squamous intraepithelial lesion group according to control group (p:0.004 and p:0.015, respectively). Disulfide level is significantly increased in the high-grade squamous intraepithelial lesion group compared to control group (p:0.004). Oxidative stress index levels in high-grade squamous intraepithelial lesion group were observed as significantly higher according to the control group (p:0.014). Ischemia-modified albumin levels in the high-grade squamous intraepithelial lesion group were observed as significantly higher compared to the control group (p:0.020). Disulfide levels are positively correlated with risk type of Human papillomavirus (r:0.420, p<0.001).Conclusion: The analysis of dynamic thiol disulfide balance revealed considerable oxidative damage in patients with Human papillomavirus -related cervical precursor lesions compared to women with ordinary cytology specimens. Therefore, investigation of thiol disulfide balance with presented method represents a new promising test for early diagnosis and management of women at high risk for cervical cancer.

The Association between Oxidative Stress and Cardiac Functions in Infants Born to Preeclamptic Mothers

2018 ◽  
Vol 36 (11) ◽  
pp. 1205-1210
Author(s):  
Didem Arman ◽  
Secil Ercin ◽  
Sevilay Topcuoğlu ◽  
Ayşem Kaya ◽  
Taner Yavuz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Premature Infants ◽  
Stress Index ◽  
Control Group ◽  
Cardiac Functions ◽  
Total Antioxidant ◽  
Echocardiographic Parameters ◽  
Oxidant Status ◽  
The Relationship ◽  
And Oxidative Stress

Objective The present study aimed to assess the global oxidant and antioxidant status in infants born to preeclamptic mothers and their correlation with cardiac functions. Study Design We compared 40 infants born to preeclamptic mothers with 40 premature infants born to normotensive mothers. We assessed the relationship between echocardiographic measurements and total antioxidant capacity (TAC) and total oxidant status (TOS) values. Results In the study group, TAC, TOS, and oxidative stress index (OSI) levels were significantly higher in the cord blood (p = 0.03, 0.04, and 0.039, respectively) than in the control group. We did not observe any correlation between echocardiographic measurements and TAC, TOS, and OSI levels in infants born to preeclamptic mothers. Conclusion Compared with the control group, despite higher TAC levels in infants born to preeclamptic mothers, concurrent elevated OSI levels reveal that the oxidant–antioxidant balance is disturbed in favor of oxidants. Furthermore, the findings of this study suggest that echocardiographic parameters are unaffected by the oxidant status.

scholarly journals Oxidative lipid, protein, and DNA damage as oxidative stress markers in vascular complications of diabetes mellitus

2011 ◽  
Vol 34 (3) ◽  
pp. 163 ◽  
Author(s):  
Omur Tabak ◽  
Remise Gelisgen ◽  
Hayriye Erman ◽  
Fusun Erdenen ◽  
Cüneyt Muderrisoglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Vascular Complications ◽  
Diabetic Group ◽  
Control Group ◽  
Diabetic Patients ◽  
Serum Samples ◽  
Type 2 Diabetic Patients

Purpose: The purpose of this study was to determine the effects of diabetic complications on oxidation of proteins, lipids, and DNA and to investigate the relationship between oxidative damage markers and clinical parameters. Methods: The study group consisted of 69 type 2 diabetic patients (20 patients without complication, 49 patients with complication) who attended internal medicine outpatient clinics of Istanbul Education and Research Hospital and 19 healthy control subjects. In serum samples of both diabetic patients and healthy subjects, 8-hydroxy-2’deoxyguanosine (8-OHdG), as a marker of oxidative DNA damage, Nε-(hexanoyl)lysine (HEL) and 15-F2t-iso-prostaglandin (15-F2t-IsoP). as products of lipooxidative damage, advanced oxidation protein products (AOPP), as markers of protein damage, and paraoxonase1 (PON1) as antioxidant were studied. Results: 15-F2t-IsoP (p < 0.005) and AOPP (p < 0.001) levels were significantly higher in diabetic group than control group while there were no significant differences in levels of 8-OHdG and HEL between the two groups. AOPP (p < 0.001) and 8-OHdG (p < 0.001) were significantly higher in diabetic group with complications compared to diabetic group without complications. Conclusions: Increased formation of free radicals and oxidative stress, under conditions of hyperglycaemia, is one of the probable causes for evolution of complications in diabetes mellitus. Our study supports the hypothesis that oxidant/antioxidant balance is disturbed in diabetic patients.

scholarly journals Salivary Biomarkers of Oxidative Stress in Children with Chronic Kidney Disease

2018 ◽  
Vol 7 (8) ◽  
pp. 209 ◽  
Author(s):  
Mateusz Maciejczyk ◽  
Julita Szulimowska ◽  
Anna Skutnik ◽  
Katarzyna Taranta-Janusz ◽  
Anna Wasilewska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Oxidative Damage ◽  
Diagnostic Value ◽  
Redox Status ◽  
Stress Index ◽  
Antioxidant Systems ◽  
Control Group ◽  
Potential Biomarker

There are still missing non-invasive biomarkers of chronic kidney disease (CKD) in children. Therefore, the aim of the study was to evaluate oxidative stress indicators in the non-stimulated (NWS) and stimulated saliva (SWS) of CKD children (n = 25) and healthy controls (n = 25). Salivary antioxidants (catalase (CAT), peroxidase (Px), superoxide dismutase (SOD), uric acid (UA), reduced glutathione (GSH), albumin), redox status (total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI)), and oxidative damage products (advanced glycation end products (AGE), advanced oxidation protein products (AOPP), malondialdehyde (MDA)) were evaluated. We have demonstrated the significantly higher activity of SWS GPx and SOD, as well as elevated concentrations of UA and albumin in NWS and SWS of CKD children vs. the control group. TAC, TOS and OSI were significantly higher only in SWS, while oxidative damage products (AGE, AOPP and MDA) were significantly higher in both NWS and SWS of CKD children. ROC analysis showed a considerably high diagnostic value of AOPP in both NWS and SWS of CKD children compared to controls (AUC = 0.92; 0.98). CKD is responsible for disturbances in salivary antioxidant systems and oxidative damage to proteins and lipids. Salivary AOPP can be a potential biomarker of CKD in children.

scholarly journals Genetic evidence for fetal origin of transcobalamin II in human cord blood

Blood ◽  
1983 ◽  
Vol 62 (1) ◽  
pp. 234-237 ◽  
Author(s):  
HJ Porck ◽  
M Frater-Schroder ◽  
RR Frants ◽  
L Kierat ◽  
AW Eriksson
Keyword(s):  
Cord Blood ◽  
Polyacrylamide Gel Electrophoresis ◽  
Maternal Serum ◽  
Control Group ◽  
Transplacental Passage ◽  
Healthy Male ◽  
Serum Samples ◽  
Human Cord Blood ◽  
Cord Serum ◽  
Transcobalamin Ii

Abstract Phenotypes of transcobalamin II (TC2) were determined in 95 maternal- cord serum pairs in order to identify the origin of TC2 in human cord blood. Unsaturated (apo) TC2 in serum was labeled with radioactive (57Co) cobalamin (CbI) and separated into isoproteins by polyacrylamide gel electrophoresis and autoradiography. Discordancy between the maternal and the cord serum type was observed in 45% of the pairs. The results demonstrated that, at the end of pregnancy, the fetus is capable of TC2 synthesis and that there is no detectable transplacental passage of maternal apo-TC2. Presence of maternal saturated (holo) TC2 in cord serum could be excluded in 9 informative discordant pairs by exchanging endogenously bound CbI with 57Co-CbI. Our finding that TC2 in human cord serum is of fetal rather than maternal origin suggests an essential role for fetal TC2 in CbI utilization and appears to contradict the hypothesis that transplacental passage of maternal TC2 may explain the normal fetal development in cases of congenital TC2 deficiency. The total immunoreactive TC2 content in 23 maternal serum samples collected at the end of pregnancy (812 +/- 175 pM CbI equivalent) was significantly higher than in the corresponding cord sera (605 +/- 148 pM; p less than 0.001) and did not significantly differ from the value in a control group of healthy male and female adults (841 +/- 192 pM). At the end of pregnancy, the apo-TC2 content in 12 maternal serum samples (760 +/- 347 pM) was significantly higher than in the corresponding cord sera (501 +/- 254 pM; p less than 0.05) and did not significantly differ from the value in the control group (747 +/- 137 pM).

Oxidative stress in proliferative lesions of parathyroid gland

2018 ◽  
Vol 90 (5) ◽  
pp. 1-6
Author(s):  
Mariusz Deska ◽  
Oliwia Segiet ◽  
Ewa Romuk ◽  
Grzegorz Buła ◽  
Joanna Polczyk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Transferase ◽  
Antioxidant Defense System ◽  
Stress Index ◽  
Control Group ◽  
Endocrine Disorders ◽  
Transferase Activity ◽  
Lipid Hydroperoxides ◽  
Sod Activity ◽  
Sh Groups

Background: Primary hyperparathyroidism (PHPT) is one of the most common endocrine disorders and defined as excessive secretion of parathormone. PHPT is a risk factor of several cardiovascular diseases, which could be caused by alterations in oxidant-antioxidant balance. Materials and methods: Blood serum collected from 52 consecutive patients with PHPT treated surgically constituted our study material, whereas 36 healthy volunteers were our control group. Oxidative stress was evaluated in both patients and control subjects by assessment of malondialdehyde (MDA) and lipid hydroperoxides (LHP). Antioxidants were evaluated by the measurement of superoxide dismutase (SOD), ceruloplasmin (CER), catalase (CAT), sulfhydryl (SH) groups, glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione transferase activity (GST) and glutathione reductase (GR). Moreover total antioxidant capacity (TAC) and total oxidative status (TOS) were measured and oxidative stress index (OSI) was calculated. Results: OSI was increased in patients with PHPT when compared to normal controls, whereas TAC was lower in PHPT. The levels of CER, MnSOD, GR, SH groups and MDA were significantly decreased in PHPT. The levels of serum LHP, catalase and SOD were significantly higher in patients with PHPT than in healthy patients. The erythrocyte CAT activity and GST were significantly increased in patients after parathyroidectomy. The erythrocyte GR and GPx were up-regulated postoperatively, whereas SOD activity decreased. Conclusions: In PHPT there are several alterations in the balance between the production of reactive oxygen species and antioxidant defense system.

scholarly journals ISCHEMIA MODIFIED ALBUMIN LEVELS AND INCREASED OXIDATIVE STRESS IN PATIENTS WITH MULTIPLE MYELOMA / NIVOI ALBUMINA MODIFIKOVANOG ISHEMIJOM I POVI[ENI OKSIDANTNI STRES KOD PACIJENATA SA MULTIPLIM MIJELOMOM

2013 ◽  
Vol 33 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Hamit Yasar Ellidag ◽  
Esin Eren ◽  
Necat Yilmaz ◽  
Asli Bayindir
Keyword(s):  
Oxidative Stress ◽  
Multiple Myeloma ◽  
Serum Levels ◽  
Stress Index ◽  
The Novel ◽  
Ischemia Modified Albumin ◽  
Significant Difference ◽  
Relationship Of ◽  
Oxidant Status ◽  
And Oxidative Stress

Summary Background: Impaired oxidative/antioxidative balance plays an important role in the pathogenesis of many diseases, including cancer. The aim of this study was to evaluate the levels of the novel marker ischemia modified albumin (IMA) and albumin adjusted-IMA (Adj-IMA) in patients with multi- ple myeloma (MM) as well as its association with total antiox- idant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI). Methods: Forty patients with MM (18 females, 22 males; mean age 67.55±8.39 years) and forty age/sex-matched healthy persons (19 females, 21 males; mean age 66.37 ± 6.76 years) were included in this study. Serum levels of IMA, TAS, TOS were analyzed and Adj-IMA and OSI was calcu- lated. Results: Serum IMA, TOS and OSI levels were significantly higher in patients with MM compared to controls (p<0.001 all parameters). There was no significant difference for serum albumin-adjusted IMA and TAS levels between groups (p=0.83 and p=0.17 respectively). Conclusions: In this study, an impaired oxidative/antioxidant status in favor of oxidative stress was found in MM patients. This observation was not confirmed by Adj-IMA calculation. Further studies are needed to establish the relationship of IMA and oxidative stress parameters in multiple myeloma and their relationship to MM and other cancers.

scholarly journals Influence of Insulin Resistance and TNF-αon the Inflammatory Process, Oxidative Stress, and Disease Activity in Patients with Rheumatoid Arthritis

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Neide Tomimura Costa ◽  
Tatiana Mayumi Veiga Iriyoda ◽  
Ana Paula Kallaur ◽  
Francieli Delongui ◽  
Daniela Frizon Alfieri ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Insulin Resistance ◽  
Disease Activity ◽  
Inflammatory Process ◽  
Cross Sectional Study ◽  
Stress Index ◽  
Control Group ◽  
Cross Sectional ◽  
Clinical Trials Registry

The aim of this study was to evaluate the involvement of TNF-αand insulin resistance (IR) in the inflammatory process, oxidative stress, and disease activity in patients with rheumatoid arthritis (RA). This cross-sectional study included 270 subjects (control group,n=97) and RA patients (n=173). RA patients were divided into four groups: the first group without IR and not using antitumor necrosis factor-α(TNF-) (G1, IR− TNF−); the second group without IR and using anti-TNF-α(G2,IR-TNF+); the third group with IR and not using anti-TNF-α(G3, IR+TNF-); and the fourth group with IR and using anti-TNF-α(G4, IR+ TNF+). G3 and G4 had higher (p<0.05) advanced oxidation protein products (AOPPs) and oxidative stress index (OSI) compared to G1. G4 group presented higher (p<0.05) AOPPs and OSI than G2. TRAP was significantly lower in G3 compared to G1. Plasma TNF-αlevels were significantly higher in G4 and G2 compared to G1 (p<0.0001) and G3 (p<0.0001andp<0.01, resp.). The presence of insulin resistance was robustly associated with both oxidative stress and TNF-αlevels. More studies are warranted to verify if IR can be involved in therapeutic failure with TNF-αinhibitors. This trial is registered with Brazilian Clinical Trials Registry Register numberRBR-2jvj92.

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