scholarly journals Sucrose Metabolism in Plants under Drought Stress Condition: A Review

2021 ◽  
Author(s):  
Anie Thomas ◽  
R. Beena
Keyword(s):  
Drought Stress ◽  
Developmental Stages ◽  
Sucrose Concentration ◽  
Turgor Pressure ◽  
Biomass Accumulation ◽  
Sucrose Metabolism ◽  
Drought Condition ◽  
Expression Of Genes ◽  
Source To Sink ◽  
Root Shoot Ratio

Drought stress reduces photosynthetic rate and leading to depletion of the energy source and lowers the yield. Under drought stress, reduced turgor pressure cause inhibition of cell elongation and impaired mitosis leads to reduction in growth rate. Role of sucrose metabolism under drought adaptation and response of plants to stress in different tissues and at different developmental stages. Cytoplasmic sucrose synthesis is more under drought condition and there is differential expression in tolerant and susceptible cultivars. Under drought condition, plant start consuming its own sink for its survival thus reducing sucrose concentration. But reduction in sucrose concentration is less in drought tolerant plants. Drought stress induced an increase of the root/shoot ratio, which was due to the increased inhibition of biomass accumulation of shoots compared to roots. Drought stress enhanced the activities of sucrose metabolic enzymes and up-regulated the expression of genes such as SPS, SuSy and INV. In addition, drought stress up-regulated the expression levels of SWEET and SUC and promoted the transport of sucrose from source to sink.

scholarly journals The Quorum Sensing Auto-Inducer 2 (AI-2) Stimulates Nitrogen Fixation and Favors Ethanol Production over Biomass Accumulation in Zymomonas mobilis

2021 ◽  
Vol 22 (11) ◽  
pp. 5628
Author(s):  
Valquíria Campos Alencar ◽  
Juliana de Fátima dos Santos Silva ◽  
Renata Ozelami Vilas Boas ◽  
Vinícius Manganaro Farnézio ◽  
Yara N. L. F. de Maria ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Quorum Sensing ◽  
Ethanol Production ◽  
N2 Fixation ◽  
Energy Demand ◽  
Zymomonas Mobilis ◽  
Biomass Accumulation ◽  
High Energy ◽  
Gram Negative ◽  
Expression Of Genes

Autoinducer 2 (or AI-2) is one of the molecules used by bacteria to trigger the Quorum Sensing (QS) response, which activates expression of genes involved in a series of alternative mechanisms, when cells reach high population densities (including bioluminescence, motility, biofilm formation, stress resistance, and production of public goods, or pathogenicity factors, among others). Contrary to most autoinducers, AI-2 can induce QS responses in both Gram-negative and Gram-positive bacteria, and has been suggested to constitute a trans-specific system of bacterial communication, capable of affecting even bacteria that cannot produce this autoinducer. In this work, we demonstrate that the ethanologenic Gram-negative bacterium Zymomonas mobilis (a non-AI-2 producer) responds to exogenous AI-2 by modulating expression of genes involved in mechanisms typically associated with QS in other bacteria, such as motility, DNA repair, and nitrogen fixation. Interestingly, the metabolism of AI-2-induced Z. mobilis cells seems to favor ethanol production over biomass accumulation, probably as an adaptation to the high-energy demand of N2 fixation. This opens the possibility of employing AI-2 during the industrial production of second-generation ethanol, as a way to boost N2 fixation by these bacteria, which could reduce costs associated with the use of nitrogen-based fertilizers, without compromising ethanol production in industrial plants.

scholarly journals Transcriptome analysis identifies differentially expressed genes in the progenies of a cross between two low phytic acid soybean mutants

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hangxia Jin ◽  
Xiaomin Yu ◽  
Qinghua Yang ◽  
Xujun Fu ◽  
Fengjie Yuan
Keyword(s):  
Developmental Stage ◽  
Phytic Acid ◽  
Differentially Expressed Genes ◽  
Transcriptome Analysis ◽  
Defense Mechanisms ◽  
Developmental Stages ◽  
Sucrose Metabolism ◽  
Differentially Expressed ◽  
Sequencing Analysis ◽  
Seed Developmental Stage

AbstractPhytic acid (PA) is a major antinutrient that cannot be digested by monogastric animals, but it can decrease the bioavailability of micronutrients (e.g., Zn and Fe). Lowering the PA content of crop seeds will lead to enhanced nutritional traits. Low-PA mutant crop lines carrying more than one mutated gene (lpa) have lower PA contents than mutants with a single lpa mutant gene. However, little is known about the link between PA pathway intermediates and downstream regulatory activities following the mutation of these genes in soybean. Consequently, we performed a comparative transcriptome analysis using an advanced generation recombinant inbred line with low PA levels [2mlpa (mips1/ipk1)] and a sibling line with homozygous non-mutant alleles and normal PA contents [2MWT (MIPS1/IPK1)]. An RNA sequencing analysis of five seed developmental stages revealed 7945 differentially expressed genes (DEGs) between the 2mlpa and 2MWT seeds. Moreover, 3316 DEGs were associated with 128 metabolic and signal transduction pathways and 4980 DEGs were annotated with 345 Gene Ontology terms related to biological processes. Genes associated with PA metabolism, photosynthesis, starch and sucrose metabolism, and defense mechanisms were among the DEGs in 2mlpa. Of these genes, 36 contributed to PA metabolism, including 22 genes possibly mediating the low-PA phenotype of 2mlpa. The expression of most of the genes associated with photosynthesis (81 of 117) was down-regulated in 2mlpa at the late seed developmental stage. In contrast, the expression of three genes involved in sucrose metabolism was up-regulated at the late seed developmental stage, which might explain the high sucrose content of 2mlpa soybeans. Furthermore, 604 genes related to defense mechanisms were differentially expressed between 2mlpa and 2MWT. In this study, we detected a low PA content as well as changes to multiple metabolites in the 2mlpa mutant. These results may help elucidate the regulation of metabolic events in 2mlpa. Many genes involved in PA metabolism may contribute to the substantial decrease in the PA content and the moderate accumulation of InsP3–InsP5 in the 2mlpa mutant. The other regulated genes related to photosynthesis, starch and sucrose metabolism, and defense mechanisms may provide additional insights into the nutritional and agronomic performance of 2mlpa seeds.

scholarly journals Comprehensive analysis and identification of drought-responsive candidate NAC genes in three semi-arid tropics (SAT) legume crops

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Sadhana Singh ◽  
Himabindu Kudapa ◽  
Vanika Garg ◽  
Rajeev K. Varshney
Keyword(s):  
Drought Stress ◽  
Candidate Genes ◽  
Developmental Stages ◽  
Biological Activities ◽  
Expression Patterns ◽  
Genome Wide ◽  
Legume Crops ◽  
Root Tissues ◽  
Semi Arid ◽  
Comprehensive Study

Abstract Background Chickpea, pigeonpea, and groundnut are the primary legume crops of semi-arid tropics (SAT) and their global productivity is severely affected by drought stress. The plant-specific NAC (NAM - no apical meristem, ATAF - Arabidopsis transcription activation factor, and CUC - cup-shaped cotyledon) transcription factor family is known to be involved in majority of abiotic stresses, especially in the drought stress tolerance mechanism. Despite the knowledge available regarding NAC function, not much information is available on NAC genes in SAT legume crops. Results In this study, genome-wide NAC proteins – 72, 96, and 166 have been identified from the genomes of chickpea, pigeonpea, and groundnut, respectively, and later grouped into 10 clusters in chickpea and pigeonpea, while 12 clusters in groundnut. Phylogeny with well-known stress-responsive NACs in Arabidopsis thaliana, Oryza sativa (rice), Medicago truncatula, and Glycine max (soybean) enabled prediction of putative stress-responsive NACs in chickpea (22), pigeonpea (31), and groundnut (33). Transcriptome data revealed putative stress-responsive NACs at various developmental stages that showed differential expression patterns in the different tissues studied. Quantitative real-time PCR (qRT-PCR) was performed to validate the expression patterns of selected stress-responsive, Ca_NAC (Cicer arietinum - 14), Cc_NAC (Cajanus cajan - 15), and Ah_NAC (Arachis hypogaea - 14) genes using drought-stressed and well-watered root tissues from two contrasting drought-responsive genotypes of each of the three legumes. Based on expression analysis, Ca_06899, Ca_18090, Ca_22941, Ca_04337, Ca_04069, Ca_04233, Ca_12660, Ca_16379, Ca_16946, and Ca_21186; Cc_26125, Cc_43030, Cc_43785, Cc_43786, Cc_22429, and Cc_22430; Ah_ann1.G1V3KR.2, Ah_ann1.MI72XM.2, Ah_ann1.V0X4SV.1, Ah_ann1.FU1JML.2, and Ah_ann1.8AKD3R.1 were identified as potential drought stress-responsive candidate genes. Conclusion As NAC genes are known to play role in several physiological and biological activities, a more comprehensive study on genome-wide identification and expression analyses of the NAC proteins have been carried out in chickpea, pigeonpea and groundnut. We have identified a total of 21 potential drought-responsive NAC genes in these legumes. These genes displayed correlation between gene expression, transcriptional regulation, and better tolerance against drought. The identified candidate genes, after validation, may serve as a useful resource for molecular breeding for drought tolerance in the SAT legume crops.

scholarly journals Influence of Abscisic Acid and Sucrose on Somatic Embryogenesis in CactusCopiapoa tenuissimaRitt. formamostruosa

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
J. Lema-Rumińska ◽  
K. Goncerzewicz ◽  
M. Gabriel
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Somatic Embryos ◽  
Sucrose Concentration ◽  
Turgor Pressure ◽  
Direct Somatic Embryogenesis ◽  
Fresh Weight ◽  
High Concentration ◽  
Globular Stage ◽  
Indirect Somatic Embryogenesis

Having produced the embryos of cactusCopiapoa tenuissimaRitt. formamonstruosaat the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100 μM on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA (0, 0.1, 1 μM) and sucrose (1, 3, 5%). The results showed that a low concentration of ABA (0-1 μM) stimulates the elongation of embryos at the globular stage and the number of correct embryos in direct somatic embryogenesis, while a high ABA concentration (10–100 μM) results in growth inhibition and turgor pressure loss of somatic embryos. The indirect somatic embryogenesis study in this cactus suggests that lower ABA concentrations enhance the increase in calli fresh weight, while a high concentration of 10 μM ABA or more changes calli color and decreases its proliferation rate. However, in the case of indirect somatic embryogenesis, ABA had no effect on the number of somatic embryos and their maturation. Nevertheless, we found a positive effect of sucrose concentration for both the number of somatic embryos and the increase in calli fresh weight.

scholarly journals Effect of Elevated CO2 on Biomolecules’ Accumulation in Caraway (Carum carvi L.) Plants at Different Developmental Stages

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2434
Author(s):  
Hamada AbdElgawad ◽  
Mohammad K. Okla ◽  
Saud S. Al-amri ◽  
Abdulrahman AL-Hashimi ◽  
Wahida H. AL-Qahtani ◽  
...  
Keyword(s):  
Elevated Co2 ◽  
Developmental Stages ◽  
Co2 Enrichment ◽  
Biomass Accumulation ◽  
Antibacterial Activities ◽  
Mature Stage ◽  
Plant Metabolites ◽  
Phytochemical Content ◽  
The Impact ◽  
Phytochemical Contents

Caraway plants have been known as a rich source of phytochemicals, such as flavonoids, monoterpenoid glucosides and alkaloids. In this regard, the application of elevated CO2 (eCO2) as a bio-enhancer for increasing plant growth and phytochemical content has been the focus of many studies; however, the interaction between eCO2 and plants at different developmental stages has not been extensively explored. Thus, the present study aimed at investigating the changes in growth, photosynthesis and phytochemicals of caraway plants at two developmental stages (sprouts and mature tissues) under control and increased CO2 conditions (ambient CO2 (a CO2, 400 ± 27 μmol CO2 mol−1 air) and eCO2, 620 ± 42 μmol CO2 mol−1 air ppm). Moreover, we evaluated the impact of eCO2-induced changes in plant metabolites on the antioxidant and antibacterial activities of caraway sprouts and mature plants. CO2 enrichment increased photosynthesis and biomass accumulation of both caraway stages. Regarding their phytochemical contents, caraway plants interacted differently with eCO2, depending on their developmental stages. High levels of CO2 enhanced the production of total nutrients, i.e., carbohydrates, proteins, fats and crude fibers, as well as organic and amino acids, in an equal pattern in both caraway sprouts and mature plants. Interestingly, the eCO2-induced effect on minerals, vitamins and phenolics was more pronounced in caraway sprouts than the mature tissues. Furthermore, the antioxidant and antibacterial activities of caraway plants were enhanced under eCO2 treatment, particularly at the mature stage. Overall, eCO2 provoked changes in the phytochemical contents of caraway plants, particularly at the sprouting stage and, hence, improved their nutritive and health-promoting properties.

The transcription factor ZmNAC49 reduces stomatal density and improves drought tolerance in maize

2020 ◽  
Author(s):  
Yang Xiang ◽  
Xiujuan Sun ◽  
Xiangli Bian ◽  
Tianhui Wei ◽  
Tong Han ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Stomatal Conductance ◽  
Drought Tolerance ◽  
Transpiration Rate ◽  
Transcriptional Activation ◽  
Stomatal Density ◽  
Stomatal Development ◽  
Expression Of Genes ◽  
Growth Development

Abstract Drought stress severely limits the growth, development, and productivity of crops, and therefore understanding the mechanisms by which plants respond to drought is crucial. In this study, we cloned a maize NAC transcription factor, ZmNAC49, and identified its function in response to drought stress. We found that ZmNAC49 is localized in the nucleus and has transcriptional activation activity. ZmNAC49 expression is rapidly and strongly induced by drought stress, and overexpression enhances stress tolerance in maize. Overexpression also significant decreases the transpiration rate, stomatal conductance, and stomatal density in maize. Detailed study showed that ZmNAC49 overexpression affects the expression of genes related to stomatal development, namely ZmTMM, ZmSDD1, ZmMUTE, and ZmFAMA. In addition, we found that ZmNAC49 can directly bind to the promoter of ZmMUTE and suppress its expression. Taken together, our results show that the transcription factor ZmNAC49 represses ZmMUTE expression, reduces stomatal density, and thereby enhances drought tolerance in maize.

Characterization and Expression of Genes Encoding Superoxide Dismutase in the Oriental Armyworm, Mythimna separata (Lepidoptera: Noctuidae)

2019 ◽  
Vol 112 (5) ◽  
pp. 2381-2388 ◽  
Author(s):  
Hong-Bo Li ◽  
Chang-Geng Dai ◽  
Yong-Fu He ◽  
Yang Hu
Keyword(s):  
Superoxide Dismutase ◽  
Population Density ◽  
Developmental Stages ◽  
Mythimna Separata ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Key Factor ◽  
Important Pest ◽  
Oriental Armyworm

Abstract Superoxide dismutase (SOD) is an antioxidant metalloenzyme that catalyzes the dismutation of the superoxide anion O2− to O2 and H2O2. Many studies have focused on the role of SOD in response to abiotic stress, but its role during biotic stress, such as changes in organismal population density, has rarely been investigated. The oriental armyworm, Mythimna separata, is an economically important pest that exhibits phenotypic changes in response to population density. Solitary and gregarious phases occur at low and high population density, respectively. To examine the role of SODs in response to population density stress, we cloned two genes encoding SOD, MsCuZnSOD and MsMnSOD, and compared their expression in solitary and gregarious phases of M. separata. The MsCuZnSOD and MsMnSOD ORFs were 480 and 651 bp and encoded predicted protein products of 159 and 216 amino acids, respectively. The two SODs contained motifs that are typical of orthologous proteins. Real-time PCR indicated that the two SOD genes were expressed throughout developmental stages and were significantly upregulated in more mature stages of gregarious M. separata. Expression of the two SOD genes in various tissues of sixth-instar larvae was higher in gregarious versus solitary insects. Furthermore, expression of the SOD genes was significantly upregulated in response to crowding in solitary individuals, but suppressed in gregarious insects subjected to isolation. Collectively, these results suggest that population density may be key factor in the induction of SOD genes in M. separata.

Drought-induced changes in the accumulation of boiling-soluble proteins (p40, GST, HSP90) in the grains of drought-tolerant and drought-sensitive cultivars of Triticum aestivum

2015 ◽  
Vol 66 (9) ◽  
pp. 904 ◽  
Author(s):  
Gurmeen Rakhra ◽  
Arun Dev Sharma ◽  
Jatinder Singh
Keyword(s):  
Triticum Aestivum ◽  
Drought Stress ◽  
Crop Production ◽  
Developmental Stages ◽  
Stress Adaptation ◽  
Dependent Manner ◽  
Drought Tolerant ◽  
Sds Page ◽  
Drought Conditions ◽  
Specific Regulation

Approximately 70% of crop yield losses are caused by abiotic stresses, with drought being the most serious threat to crop production in many areas of the world. Plants have developed physiological and biochemical responses at multiple levels to allow them to grow and survive under drought stress. Among these, hydrophilins (BSPs, proteins soluble after boiling), representing 0.2% of the total genome, play an important role in the stress adaptation in plants. In this study, we examined the effect of drought on BSPs at different developmental stages of leaves and seeds in drought-tolerant (cv. PBW 175) and drought-susceptible (cv. PBW 621) cultivars of Triticum aestivum. The BSP profiles of seeds were outlined via SDS-PAGE followed by immunoblot analysis using anti-HSP (heat shock protein-90), anti-GST (glutathione S-transferases) and anti-p40 (protein 40). In SDS-PAGE profile, BSPs were detected in a genotype- and treatment-dependent manner. Notably, no BSPs were detected in shoots at any stage, whereas in seeds, many BSPs were detected, indicating organ-specific regulation of BSPs. In western blotting, the induced accumulation of protein bands Bsp40-51 and 59 and presence of differential band of BsHSP44 under drought conditions was observed only in tolerant cv. PBW 175, not in sensitive cv. PBW 621, indicating the roles of such proteins in drought-stress adaptation. BSPs were accumulated at different developmental stages in a cultivar- and stage-dependent manner. The induced expression of different BSPs under drought conditions in tolerant cv. PBW 175 implies the relevance of these BSPs under drought conditions. Notably, the different BSPs were also expressed under normal growth and developmental stages at 57 and 76 days post-anthesis, implying their key role in earlier stages and maturity of grain development.

scholarly journals Calcium Application Enhances Drought Stress Tolerance in Sugar Beet and Promotes Plant Biomass and Beetroot Sucrose Concentration

2019 ◽  
Vol 20 (15) ◽  
pp. 3777 ◽  
Author(s):  
Seyed Abdollah Hosseini ◽  
Elise Réthoré ◽  
Sylvain Pluchon ◽  
Nusrat Ali ◽  
Bastien Billiot ◽  
...  
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Sugar Beet ◽  
Stress Tolerance ◽  
Mineral Nutrition ◽  
Stress Responses ◽  
Foliar Application ◽  
Sucrose Concentration ◽  
Drought Stress Tolerance

Numerous studies have demonstrated the potential of sugar beet to lose the final sugar yield under water limiting regime. Ample evidences have revealed the important role of mineral nutrition in increasing plant tolerance to abiotic stresses. Despite the vital role of calcium (Ca2+) in plant growth and development, as well as in stress responses as an intracellular messenger, its role in alleviating drought stress in sugar beet has been rarely addressed. Here, an attempt was undertaken to investigate whether, and to what extent, foliar application of Ca2+ confers drought stress tolerance in sugar beet plants exposed to drought stress. To achieve this goal, sugar beet plants, which were grown in a high throughput phenotyping platform, were sprayed with Ca2+ and submitted to drought stress. The results showed that foliar application of Ca2+ increased the level of magnesium and silicon in the leaves, promoted plant growth, height, and leaf coverage area as well as chlorophyll level. Ca2+, in turn, increased the carbohydrate levels in leaves under drought condition and regulated transcriptionally the genes involved in sucrose transport (BvSUC3 and BvTST3). Subsequently, Ca2+ enhanced the root biomass and simultaneously led to induction of root (BvSUC3 and BvTST1) sucrose transporters which eventually supported the loading of more sucrose into beetroot under drought stress. Metabolite analysis revealed that the beneficial effect of Ca2+ in tolerance to drought induced-oxidative stress is most likely mediated by higher glutathione pools, increased levels of free polyamine putrescine (Put), and lower levels of amino acid gamma-aminobutyric acid (GABA). Taken together, this work demonstrates that foliar application of Ca2+ is a promising fertilization strategy to improve mineral nutrition efficiency, sugar metabolism, redox state, and thus, drought stress tolerance.

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