scholarly journals Biolarvicidal activity of Rhizophora stylosa leaf extract against Aedes sp.

2021 ◽  
Vol 869 (1) ◽  
pp. 012062
Author(s):  
D Syahputra ◽  
S Karina ◽  
Irwan ◽  
S Agustina ◽  
M Ulfah
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Research Focus ◽  
Rhizophora Stylosa ◽  
Marine Chemistry ◽  
Qualitative Test ◽  
Banda Aceh

Abstract The research focus on biolarvicidal activity of Rhizophora Stylosa leaf extract against Aedes sp. R. stylosa leaf was collected from Gampong Pande, Banda Aceh. This research was conducted at laboratory of Marine Chemistry and Fisheries Biotechnology, Marine and Fisheries Faculty, Universitas Syiah Kuala from Desember 2020 to March 2021. The objective of this study was to figure out the secondary metabolites content in the methanol extract of the mangrove leaf and its biolarvicidal activity against Aedes sp. larvae. The secondary metabolites were screened out using phytochemical screening. The extract was tested for its biolarvicidal activity against third instar larvae of the Aedes sp. mosquito at incubation’s time of 48 hours. The biolarvicidal activity is expressed in LC50 which was determined by probit analysis using SPSS. The results of qualitative test showed that the extract contained of flavonoids, saponins, triterpenoids and tannins. Based on the results of probit analysis, the LC50 value of the extract against Aedes sp. was 858.89 ppm.

Antioxidant and acetylcholinesterase inhibitory activities of leaf extract and fractions of Albizia adianthifolia (Schumach) W.F. Wright

2017 ◽  
Vol 28 (2) ◽  
Author(s):  
Mubo A. Sonibare ◽  
Ibukun O. Ayoola ◽  
Taiwo O. Elufioye
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Phenolic Contents ◽  
Inhibitory Activities ◽  
Polar Extracts ◽  
Albizia Adianthifolia ◽  
Gallic Acid Equivalent

AbstractBackground:This study was conducted to determine the antioxidant and acetylcholinesterase inhibitory activities of the leaf extract and different fractions ofMethods:The antioxidant and acetylcholinesterase inhibitory activities of the methanol extract (CME), ethyl acetate (EAF), chloroform (CHF), andResults:Phytochemical screening revealed important classes of secondary metabolites. All extracts showed good antioxidant and acetylcholinesterase inhibitory activities. The EAF and CHF gave the highest total phenolic contents of 18.02±0.14 and 18.43±0.95 mg gallic acid equivalent per gram of extract, respectively. The polar extracts gave the highest activity in both assays with lower ICConclusions:The results of this study suggest that the leaf of

scholarly journals A New Cycloartane Glucoside from Rhizophora stylosa

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Phan Thi Thanh Huong ◽  
Chau Ngoc Diep ◽  
Nguyen Van Thanh ◽  
Vu Anh Tu ◽  
Tran Hong Hanh ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Lung Adenocarcinoma ◽  
Cell Lines ◽  
Caspase 3 ◽  
Methanol Extract ◽  
Human Cancer ◽  
Human Cancer Cell ◽  
Rhizophora Stylosa ◽  
Human Cancer Cell Lines ◽  
C Nmr

Nine secondary metabolites, including a new cycloartane glucoside, rhizostyloside (1), were isolated from a methanol extract of Rhizophora stylosa leaves through several chromatographic experiments. The structures of the compounds were determined on the basis of NMR spectroscopic (1H and 13C NMR, HSQC, HMBC, 1H-1H COSY, NOESY) and HR-ESI-MS data and by comparison with literature values. Compound 1 exhibited significant cytotoxicity against three human cancer cell lines: KB (epidermoid carcinoma), LU-1 (lung adenocarcinoma), and SK-Mel-2 (melanoma). In addition, 1 strongly activated caspase-3/7 in LU-1 cells.

scholarly journals A Preliminary Screening of the Different of Secondary Metabolites Ruku-Ruku Leaves (Ocimum tenuiflorum Linnen) in West Sumatera

2019 ◽  
Vol 20 (2) ◽  
pp. 17-24
Author(s):  
Hesty Parbuntari ◽  
Sri Benti Etika ◽  
Melindra Mulia ◽  
Elfanny Delvia
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Light Exposure ◽  
Phytochemical Screening ◽  
Preliminary Screening ◽  
West Sumatra ◽  
Ocimum Tenuiflorum ◽  
Qualitative Test

Ruku-ruku leaves are used to remove distinctive odors from fish in some cuisine of West Sumatra. The characteristic odor comes from secondary metabolites especially essential oils. Therefore, secondary metabolites in ruku-ruku leaves have the potential to be further investigated as a source of bioactive compounds. The phytochemical screening of ruku-ruku leaves is a preminilary qualitative test that needs to be done to maximise its function as a medicinal plant. Based on the result data, there are some different result of flavonoid, steroids, and triterpenoids identification. Ruku-ruku leaves from Pesisir Selatan and Padang Panjang shows less flavonoid compounds tham Payakumbuh, Batusangkar, and Lubuk Alung. Both of this city and Payakumbuh also gives less steroids and triterpenoids. The factor influencing this result is caused by several factors, namely temperature, humidity, light exposure, and salinity.

scholarly journals Antidiarrhoeal Studies of Methanol Leaf Extract of Cassia sieberiana Dc in Wistar Albino Rats

2021 ◽  
pp. 43-49
Author(s):  
Jude Nwaogu ◽  
Ibrahim Babangida Abubakar ◽  
Esther Omolade Olaosebikan
Keyword(s):  
Activated Charcoal ◽  
Castor Oil ◽  
Cardiac Glycosides ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Gastrointestinal Transit ◽  
Albino Rats ◽  
Phytochemical Screening ◽  
Standard Methods ◽  
Wistar Albino Rats

Aim: This study was aimed at evaluating the antidiarrhoeal effect methanol leaf extract of Cassia sieberiana. Methodology: The leaf extract of Cassia sieberiana was obtained by extraction using methanol and subjected to phytochemical screening using standard methods. The anti-diarrhoeal effect of methanol leaf extract of Cassia sieberiana DC was investigated in Wistar Albino rats. Results: The result of phytochemical screening revealed the presence of saponins, tannins, phenols, terpenoids, cardiac glycosides, steroids, flavonoids and anthraquinoes. The methanol extract significantly (P<.05) reduced the number of unformed faeces in castor oil induced diarrhoea in the rats. It also significantly (P<.05) reduced the gastrointestinal transit of activated charcoal as well as enteropooling in the rats used for the experiment was significantly reduced (P<.05) in the groups treated with 500 mg/kg and 750 mg/kg. Conclusion: Based on the findings of this research, the methanol leaf extract of Cassia sieberiana possess anti-diarrheal activity and therefore validates its use in traditional medicine for the treatment of diarrhoea.

scholarly journals Toxicity of Citrus mitis, Citrus aurantifolia, and Citrus maxima leaf extract toward mortality of Aedes aegypti larvae (Diptera: Culicidae)

2017 ◽  
Vol 3 (6) ◽  
pp. 41
Author(s):  
Hamidah Hamidah ◽  
Hebert Adrianto
Keyword(s):  
Aedes Aegypti ◽  
Dengue Fever ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Probit Analysis ◽  
Mosquito Larvae ◽  
Citrus Aurantifolia ◽  
Citrus Maxima ◽  
Spss Software ◽  
Significant Health

Dengue Fever is a significant health issue in Indonesia for it is always been found and unresolved since 1968. Dengue Fever is transmitted through mosquito (Aedes aegypti) bite. Resistance of mosquito larvae towards temephos as consequence of chemical larvicide consumption has been reported in several countries. One of safe and environmentally friendly efforts to control mosquito is by using herbal larvicide which produced from plants. This study examines methanol extract of Citrus mitis, Citrus aurantifolia, and Citrus maxima leaf toward mosquito larvae Ae. aegypti instar III for 24 hours. Data of larvae mortality is analyzed using probit analysis by SPSS software. The result shows that Citrus mitis has the highest toxicity with the lowest lethal concentrations (LC) that are LC50 = 1.547 ppm and LC90 = 3.328 ppm. It followed by Citrus aurantifolia and Citrus maxima respectively.Keywords: larvicide; Aedes aegypti; leaf extract; Citrus mitis; Citrus aurantifolia; Citrus maxima

Preliminary Phytochemical Screening of Leaves of Ocimum tenuiflorum

2021 ◽  
Vol 1 (30) ◽  
pp. 63-66
Author(s):  
Shweta Tyagi I.P.Pandey
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Fats And Oils ◽  
Phytochemical Screening ◽  
The People ◽  
Ocimum Tenuiflorum ◽  
Phytochemical Constituents ◽  
Clinical Drugs ◽  
Clinical Drug

Abstract-The medicinal plants are useful for healing and curing of human diseases. Over 55% of all modern clinical drugs are of natural product origin. The plant, Ocimum tenuiflorum is highly used by the people of whole world specially Indians to cure various disorders because of the presence of phytochemical constituents. Ocimum tenuiflorum plant is known to possess anticancer, antispasmodic, antiviral, insecticide, antiseptic, analgesic, antiinflammatory, antimicrobial, antistress, Immunomodulatory, hypotensive and antioxidant properties. The present study reveals that various secondary metabolites such as glycosides, alkaloids, flavonoids, phenols, terpenoids, tannins, saponins, antraquenone , fats and oils and steroids are present in different leaf extract of the plant. Keywords: Ocimum tenuiflorum , secondary metabolites, clinical drug

scholarly journals Antioksidan Daun Kumpai Mahung (Eupathorium inulifolium H.B&K)

2021 ◽  
Vol 8 (1) ◽  
pp. 94
Author(s):  
Dwi Rizki Febrianti ◽  
Rakhmadhan Niah ◽  
Novia Ariani
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Traditional Medicine ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Endemic Plants ◽  
Ic50 Value ◽  
Dpph Method

Daun Kumpai Mahung (Eupathorium inulifolium H.B & K) merupakan salah satu tumbuhan endemik Kalimantan Selatan. Secara turun temurun digunakan sebagai obat tradisional Dayak Meratus sebagai obat diare, demam, dan malaria. Tanaman ini dicurigai memiliki nilai antioksidan tinggi karena mengandung metabolit skunder fenolik. Tujuan penelitian ini untuk mengetahui aktivitas antioksidan ekstrak metanol daun E. inulifolium serta nilai IC50-nya. Penelitian ini menggunakan metode DPPH dengan instrumen spektofotometri UV-vis dengan panjang gelombang 517 nm. Dari hasil perhitungan dan replikasi nilai IC50 yang didapat sebesar 38,9 ppm. Ekstrak daun E. inulifolium memiliki aktivitas antioksidan yang sangat kuat dalam meredam radikal bebas. Kata Kunci: Daun, Potensi Antioksidan, Endemik, IC50, Ekstrak Etanol, Fenolik Kumpai Mahung (Eupathorium inulifolium H.B & K) leaves are one of the endemic plants of South Kalimantan. From generation to generation it is used as a traditional medicine for Dayak Meratus as a medicine for diarrhea, fever, and malaria. This plant is suspected of having high antioxidant value because it contains phenolic secondary metabolites. The purpose of this study was to determine the antioxidant activity of the methanol extract of E. inulifolium leaves and its IC50 value. This study used the DPPH method with spectophotometer UV-vis instrument at wavelength of 517 nm. From the calculation and replication, the IC50 value obtained is 38.9 ppm. E. inulifolium leaf extract has very strong antioxidant activity in reducing free radicals. 

scholarly journals Antimicrobial activities and phytochemical screening of leaf extract of Mikania micrantha H.B.K

2018 ◽  
Vol 30 ◽  
pp. 274-286
Author(s):  
Anjana Devkota ◽  
Anita Sahu
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Mikania Micrantha ◽  
Crude Leaf Extract

Antimicrobial activities and phytochemical screening of leaves of Mikania micrantha was tested in laboratory against phytopathogenic fungi and human pathogenic bacteria. The leaves samples were extracted in distilled water and methanol. The crude extracts of leaves were assessed in-vitro for antimicrobial activity using different concentrations (50, 100, 150,200, and 250 mg/ml) against five fungal strains (viz. Sclerotium rolfsii, Phytopthora capsici, Alternaria brassicae, Fusarium oxysporum and Botrytis cinerea) and six bacterial strains (Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, Bacillus subtilis, Enterococcusfaecalis and Staphylococcus aureus). Antifungal activity was tested by Poison Food Technique and Linear Mycelium growth Reduction (LMGR) percentage was determined. In crude extractof M. micrantha, the highest LMGR percentage was found in F. oxysporum in both distilledwater and methanol extract. Antibacterial activity was carried out by Disc Diffusion method. In the crude leaf extract of M. micrantha Gram negative bacteria was found more resistant than Gram positive bacteria. Methanol extract was found more effective in determining the Zone of Inhibition for all the strains of bacteria in all the concentrations. The plant extracts were found more effective in showing antibacterial activity than antifungal activity. The phytochemicalscreening revealed that the selected species contained tannin, saponins, alkaloid, flavonoid, cardiac glycosides and terpenoids. This result supports the potential of this plant species used as a new chemotherapeutic drug.

scholarly journals Effectiveness of antioxidant and anticoligenase activity of 70% ethanolic extracts of Kemangi leaves (Ocimum Basilicum)

2019 ◽  
Vol 4 (4) ◽  
pp. 13-18
Author(s):  
Hanjaya ◽  
Ermi Girsang ◽  
Ali Napiah Nasution ◽  
Sukirman Lie
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Power Method ◽  
Phytochemical Screening ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Collagenase Inhibition

Free radicals are one form of reactive oxygen compounds, which are generally known as compounds that have unpaired electrons. Kemangi leaves are used as an aphrodisiac because they contain araginin which can strengthen sperm resistance and prevent infertility. Besides araginin, Kemangi leaves also contain other secondary metabolites such as essential oils, phytosterols, alkaloids, phenolic compounds, tannins, lignin, saponins, flavonoids, terpenoids and anthraquinones. Phytochemical screening results of 70% ethanol extract of kemangi herbs themselves showed the presence of secondary metabolites of flavonoids, saponins, tannins and triterpenoids / steroids. In this study, antioxidant activity was tested using the FRAP (Ferric Reducing Antioxidant Power) method, with concentrations of kemangi leaf extract and comparative compounds Euginol each concentration of 1000 μL/ml, 5000 μL/ml, 250 μL/ml, and 125 μL/ml, 62.50 μL/ml, and 31.25 μL/ml measured at 745 nm wavelength and collagenase inhibition test with ethanol extract of kemangi leaves with a comparison of eugenol compounds measured at a wavelength of 335 nm. The FRAP antioxidant activity results obtained based on IC50 Eugenol value of 261.36 μg/ml and Kemangi leaf extract at 111.32 μg/ml, antolagenagenase from Eugenol at 255.32 μg/ml and Kemangi leaf extract at 110.65 μ/ml. 

scholarly journals Phytochemical Screening and Cytotoxicity Evaluation of Ethanolic Extract of Hypericum cordifolium (choisy) Leaves

2019 ◽  
Vol 40 ◽  
pp. 19-24
Author(s):  
Deepak Basyal ◽  
Netra Lal Bhandari
Keyword(s):  
Brine Shrimp ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Probit Analysis ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Assay ◽  
Phytochemical Screening ◽  
Soxhlet Apparatus ◽  
Leaf Powder

Hypericum cordifolium is a medicinal plant traditionally used for the treatment of the menstrual disorder, backache, dislocation of bone, fever, diarrhea and dysentery due to the presence of potential bioactive secondary metabolites. Ethanolic extract of dried leaf powder was prepared using the Soxhlet apparatus, followed by qualitative phytochemical screening with different reagents. In-vitro cytotoxicity assay was screened by using brine shrimp bioassay and LC50 was calculated by probit analysis. Alkaloids, glycosides, amino acids, and fixed oils were present at very minute level whereas saponin, phenol and flavonoids were present at significant level. Cytotoxicity assay of the leaf extract showed significant results (p<0.05).LC50   of 273 ppm, which was less than 1000 ppm, showing that the plant is toxic. The study revealed that the plant possessed medicinal values, which could justify the ethno-medicinal importance scientifically. This research has pointed out further possibilities of works to be done on this virgin plant creating the opportunity for scientific researches to give birth of potent lead molecules.

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