scholarly journals Qualitative Study of Bioactive Components of Dill (Anethum graveolens L.) From Northern Morocco

2016 ◽  
Vol 12 (27) ◽  
pp. 335
Author(s):  
Saloua Kerrouri ◽  
L.A. Lrhorfi ◽  
Sammama Amal ◽  
Elyahyaou Ouafae ◽  
Ould abdellahi Lella ◽  
...  
Keyword(s):  
Bioactive Molecules ◽  
Phytochemical Analysis ◽  
Staining Reaction ◽  
Bioactive Components ◽  
Anethum Graveolens ◽  
Underground Part ◽  
Important Place ◽  
Extraction Solvent ◽  
Thin Layer Chromatographic Analysis ◽  
Different Parts

This work aims to extract and identify bioactive molecules of the plant Anethum graveolens, commonly known as Dill, which occupies an important place in Moroccan vegetation. In order to optimise their later use in the field of herbal medicine and nutrition, a focus on the phytochemical analysis of an extract from different parts of the plant were examined by using preliminary tests and staining reaction precipitation by the use of specific reagents, or by thin layer chromatographic analysis (TLC). Phytochemical tests, different organs, aerial and underground part (on different areas of the plant), revealed the richness of this plants tannins, reducing compounds, alkaloids, coumarins, terpenoids, sterols, flavonoids, irridoides, and this screening has allowed us to show that for the extraction of polyphenols, methanol is the best extraction solvent.

scholarly journals Dendropanax morbiferus leaf extract facilitates oligodendrocyte development

2019 ◽  
Vol 6 (6) ◽  
pp. 190266
Author(s):  
Ji-Young Kim ◽  
Ju-Young Yoon ◽  
Yuki Sugiura ◽  
Soo-Kyoung Lee ◽  
Jae-Don Park ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Expression Patterns ◽  
Bioactive Molecules ◽  
Phytochemical Analysis ◽  
Bioactive Components ◽  
Multiple Components ◽  
Effective Components ◽  
Anti Inflammatory ◽  
Oligodendrocyte Development ◽  
Myelin Gene

Treatment of multiple sclerosis is effective when anti-inflammatory, neuroprotective and regenerative strategies are combined. Dendropanax morbiferus ( DM ) has anti-inflammatory, anti-oxidative properties, which may be beneficial for multiple sclerosis. However, there have been no reports on the effects of DM on myelination, which is critical for regenerative processes. To know whether DM benefits myelination, we checked differentiation and myelination of oligodendrocytes (OLs) in various primary culture systems treated with DM leaf EtOH extracts or control. DM extracts increased the OL membrane size in the mixed glial and pure OL precursor cell (OPC) cultures and changed OL-lineage gene expression patterns in the OPC cultures. Western blot analysis of DM -treated OPC cultures showed upregulation of MBP and phosphorylation of ERK1/2. In myelinating cocultures, DM extracts enhanced OL differentiation, followed by increased axonal contacts and myelin gene upregulations such as Myrf, CNP and PLP. Phytochemical analysis by LC-MS/MS identified multiple components from DM extracts, containing bioactive molecules such as quercetin, cannabidiol, etc. Our results suggest DM extracts enhance OL differentiation, followed by an increase in membrane size and axonal contacts, thereby indicating enhanced myelination. In addition, we found that DM extracts contain multiple bioactive components, warranting further studies in relation to finding effective components for enhancing myelination.

Bryophyllum Pinnatum: A Review on Medicinal Benefits and Potent Bioactive Molecules

2020 ◽  
Vol 16 (7) ◽  
pp. 978-992
Author(s):  
Punit Kumar ◽  
Sujata Malik ◽  
Kashyap K. Dubey
Keyword(s):  
Web Of Science ◽  
Folk Medicine ◽  
Bioactive Molecules ◽  
Phytochemical Analysis ◽  
Traditional Herbal Medicine ◽  
Pharmacological Activities ◽  
Online Databases ◽  
Natural Remedies ◽  
Medicinal Properties ◽  
Bryophyllum Pinnatum

Background: Since its inception, people are using herbal extracts as natural remedies for the treatment of various diseases. Bryophyllum pinnatum, also known as the air plant, is a well-recognized herb used in folk medicine. It is native to Madagascar and further planted in tropical and subtropical areas around the world. It is known for the profusion of miniature plantlets which arise from the margins of phylloclade. Objective: The aim of this review is to provide the information regarding local and traditional uses, pharmacological activities and different phytochemicals reported from leaves extracts of Bryophyllum pinnatum. Methods: This review article contains a detailed survey of the literature about Bryophyllum pinnatum available in different online databases, such as; PubMed, Web of Science, Science Direct, Elsevier, and Google Scholar, etc. In this review, authors have focused on ethnopharmacological importance and phytochemicals present in Bryophyllum pinnatum and their structure. The structures of the phytochemical were prepared by the ChemDraw tool. Results: This plant is used as a traditional herbal medicine around the globe due to medicinal properties like; anthelmintic, immunosuppressive, hepatoprotective, antinociceptive, anti-inflammatory, antidiabetic, nephroprotective, antioxidant, antimicrobial, analgesic, anticonvulsant and antipyretic. Phytochemical analysis revealed the presence of many bioactive compounds like; alkaloids, flavonoids, triterpenes, steroids, glycosides, bufadienolides, lipids, and organic acids, etc. which are associated with different medicinal properties. Conclusion: Bryophyllum pinnatum possesses diverse pharmacological importance and remarkable medicinal properties. Investigators have reported a large number of phytochemicals exhibiting different medicinal properties and correlation of medicinal properties.

scholarly journals Identificación, establecimiento in vitro y análisis fitoquímico preliminar de especies silvestres de ñame (Dioscorea spp.) empleadas con fines medicinales

2015 ◽  
Vol 17 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Víctor Andrés Ramos Duarte ◽  
Silvia Lizette Bustamante, R. ◽  
Javier Rincón Velandia ◽  
Maritza Adelina Rojas Cardozo ◽  
Lauren Raz ◽  
...  
Keyword(s):  
Culture Media ◽  
Phytochemical Analysis ◽  
Extraction Solvent ◽  
Palabras Clave ◽  
Edible Species ◽  
Medicinal Use ◽  
Advanced Analysis ◽  
Layer Chromatography ◽  
Wild Yam

<strong><strong>Título en ingles: Identification, <em>in vitro</em> establishment and preliminary phytochemical analysis of wild yam (<em>Dioscorea spp</em>.) used for medicinal purposes</strong></strong><p><strong><strong><strong>Título corto: Identificación, establecimiento <em>in vitro </em>y análisis fitoquímico preliminar de especies silvestres de ñame</strong></strong></strong></p><p><strong>Resumen:  </strong>Tubérculos del género<em> Dioscorea </em>comercializados con fines medicinales, fueron recolectados con el propósito de lograr su establecimiento a condiciones <em>in vitro. </em>Previamente se lograron identificar taxonómicamente las especies y por medio de análisis fitoquímicos demostrar su potencial farmacéutico. El material recolectado fue identificado como <em>Dioscorea coriacea, D. lehmannii, D. meridensis, D. polygonoides </em>y una especie comestible<em> D. trifida</em>. Tubérculos recolectados de centros de acopio y traídos de campo fueron lavados, desinfectados, asperjados con Ácido Giberélico (AG<sub>3</sub>) y sembrados en sustrato BM-2®, en invernadero a 18°C día y 10°C noche. Los tubérculos completos o por secciones fueron almacenados en bolsas herméticas a temperatura ambiente. Posteriormente se desinfectó material vegetal de las especies <em>D. coriacea, D. lehmannii, D. meridensis y D polygonoides, </em>seleccionando explantes de brotes sanos (<em>D. coriacea / </em>laboratorio) para su establecimiento. Se evaluaron tres medios de cultivo para establecimiento, el que presentó los mejores resultados fue Medio Murashige &amp; Skoog (1962) suplementado con BAP 1 mL/L, AG<sub>3</sub> 1 mL/L y Putrescina 2 mL/L. Para la extracción y análisis de metabolitos secundarios se utilizaron tubérculos de <em>D. coriacea, D. lehmannii</em> y <em>D. polygonoides</em>, empleando como solvente de extracción metanol. Se  encontró mayor concentración de extracto vegetal en <em>D. coriacea </em>(54%), y mediante cromatografía en capa delgada (CCD), se confirmó la presencia de saponinas, que resultó mayor en comparación con <em>D. polygonoides</em> especie reconocida por su alto contenido de saponinas. Estos resultados permitirán realizar análisis más avanzados de los compuestos presentes y plantear su propagación masiva en condiciones <em>in vitro</em>. </p><p><strong>Palabras clave:</strong> diosgenina, micropropagación, ñame silvestre, cultivo de tejidos vegetales, saponinas, fitoquímica.</p><p><strong>Abstract:  </strong>Wild tubers of the genus<em> Dioscorea</em> sold for medicinal use were collected for the purpose of achieving its establishment under <em>in vitro</em> conditions. First we taxonomically identified the species and through phytochemical analysis demonstrated pharmaceutical potential. The material collected was identified as <em>Dioscorea coriacea</em>, <em>D. lehmannii</em>, <em>D. meridensis</em>, <em>D. polygonoides</em> and the edible species <em>D. trifida</em>. Tubers collected from wholesale distributors and from the field were washed, disinfected, sprayed with Gibberellic Acid (GA<sub>3</sub>) and planted in substrate BM-2®, in a greenhouse at 18 ° C during the day and 10 ° C overnight. Whole tubers or sections thereof were stored in sealed bags at room temperature. Subsequently plant material of the species <em>D. coriacea, D. lehmannii, D. </em>meridensis<em> </em>and<em> D. polygonoides</em> was disinfected and healthy buds (<em>D. coriacea</em> / laboratory) were selected for <em>in vitro</em> establishment. Three different culture media were evaluated for establishment; that which presented the best results was the Murashige &amp; Skoog (1962) medium, supplemented with BAP 1 mL / L, GA<sub>3</sub> 1 mL / L and Putrescin 2 mL / L. For the collection and analysis of secondary metabolites, tubers of <em>D. coriacea</em>, <em>D. lehmannii</em> and <em>D. polygonoides</em> were used, using methanol as the extraction solvent. The highest concentration of plant extract, 54%, was found in<em> D. coriacea</em>, a higher value than that of <em>D. polygonoides, </em>which had been reported previously; the presence of saponins was confirmed by thin layer chromatography (TLC). These results will enable more advanced analysis of the present compounds and enhance their mass propagation under <em>in vitro</em> conditions.</p><p><strong>Key words:</strong> diosgenin, micropropagation, wild yam, tissue culture, saponins, phitochemistry.</p><p><strong>Recibido: </strong>agosto 20 de 2014<strong>      Aprobado: </strong>abril 20 de 2015</p>

DETERMINATION OF POLYPHENOLIC COMPOUNDS OF LYSIMACHIA NUMMULARIA L.

2021 ◽  
Author(s):  
Suciu Felicia ◽  
Roșca Adrian Cosmin ◽  
Lupu Carmen ◽  
Popescu Antoanela ◽  
Badea Victoria
Keyword(s):  
Extraction Process ◽  
Polyphenolic Compounds ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Important Place ◽  
Spectrophotometric Methods ◽  
Total Polyphenol Content ◽  
History Of ◽  
Different Parts

"The history of medicinal plants is associated with the evolution of civilization. In all regions of the world, the history of nations shows that these plants have always occupied an important place in medicine, in cosmetic products, and culinary preparations. The paper aims to determine the total polyphenols in different parts of the species Lysimachia nummularia L. In our study, we focused on the extraction of polyphenolic compounds in different solvents. The solvents used in the extraction were: 40% ethanol, concentrated methanol, in water. The total polyphenol content was determined by spectrophotometric methods, a method from the European Pharmacopoeia 10.0, with minor modifications. The total polyphenol content of different extracts varied depending on the extraction process. Different parts of the plant and different solvents were used in the determinations carried out to establish the optimal extraction method for the organs of Lysimachia nummularia L."

scholarly journals IN VITRO ANTIOXIDANT AND PHYTOCHEMICAL ANALYSIS OF DIFFERENT PARTS OF SIMANA LAGI APPLE ([MALUS DOMESTICA BORKH.] “SIMANA LAGI”) GROWN IN EAST JAVA-INDONESIA

2017 ◽  
Vol 10 (11) ◽  
pp. 41
Author(s):  
Irda Fidrianny ◽  
Dhella Fitrani ◽  
Siti Kusmardiyani ◽  
Defri Rizaldy ◽  
Komar Ruslan
Keyword(s):  
Malus Domestica ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Malus Domestica Borkh ◽  
Dpph Method ◽  
Phenolic And Flavonoid ◽  
Different Parts

  Objectives: The goals of this research were to observe antioxidant properties from different parts of Simana lagi apple ([Malus domestica Borkh.] “Simana lagi”) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzthiazoline-6- sulfonic acid (ABTS) and correlation of total phenolic and flavonoid content with their inhibitory concentration 50 (IC50) of DPPH and IC50 of ABTS.Methods: Each sample was extracted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities were tested using DPPH and ABTS assays, determination of total phenolic, and flavonoid content were carried out by ultraviolet-visible spectrophotometry and correlation with their IC50 of DPPH and IC50 of ABTS activities were analyzed by Pearson’s method.Results: The ethanolic leaves extract (LV3) of Simana lagi apple also exposed the highest total phenolic content (TPC) (13.88 g gallic acid equivalents/100 g), while the highest total flavonoid content was presented by ethyl acetate extract (LV2) (7.21 g QE/100 g). The lowest IC50 of DPPH scavenging activity 0.19 μg/ml, and the lowest IC50 of ABTS scavenging activity 0.15 μg/ml was given by ethanolic flesh extract (FL3) of Simana lagi apple. There were significantly negative correlation between TPC in all different parts extracts of Simana lagi apple with their IC50 of DPPH and IC50 of ABTS.Conclusions: All different parts extracts of Simana lagi apple were categorized as very strong antioxidant by DPPH and ABTS method (except n-hexane peels extract and n-hexane LV by DPPH method, and n-hexane FE by ABTS method). The major contributor in antioxidant activities of peels, flesh and leaves extracts of Simana lagi apple by DPPH and ABTS methods were phenolic compounds. Antioxidant activities of peels, flesh and leaves extracts of Simana lagi apple showed linear results by DPPH and ABTS methods.

Phytochemical Screening, Isolation, and Structure Elucidation of the Radish (Raphanus sativus Linn.) Bulb Ethanolic Extract Using Gas Chromatography- Mass Spectrometry (GC-MS)

10.17158/233 ◽  
2012 ◽  
Vol 18 (1) ◽  
Author(s):  
Ma. Eva C. San Juan ◽  
Ramchand S. Jumala ◽  
Karen Hope G. Niasca
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Raphanus Sativus ◽  
Ethanolic Extract ◽  
Bioactive Molecules ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Chromatography Mass Spectrometry ◽  
Long Chain

Nowadays, plant is being used as a biofuel, a probable source for a pesticide and it is valued for its pharmacological effects. Hence, the researchers engaged in the scientific approach to provide explanation to these claims. The study focused on the phytochemical screening of the ethanolic extract of radish bulbs (Raphanus sativus). It further dealt on the isolation and determination of the molecular masses to provide probable structures and associated molecular properties for its constituents.<br />Alkaloids, flavonoids, saponins and tannins were analyzed. Further, this effort involved analysis through Thin Layer Chromatography (TLC) using the mobile phase of Toluene: Chloroform (9:11) and Gas Chromatography- Mass Spectrometry (GC-MS) to analyze the first spot (Retention Factor= 0.667).<br />Phytochemical analysis showed no tannins. Using TLC, it revealed two spots having Rf values of 0.667 and 0.507, respectively. Using GC-MS method, 24 different molecules were isolated and analyzed of which only 23 were given probable structures. The 23 molecules constituted 11 long-chain alkanes, 4 long-chain alkyl esters, 2 aromatic ketones, a benzyl halide, an aryl ether, a quinone, an aromatic aldehyde, a long chain aromatic alkane, and a long-chain alkyl nitrile. With this finding, the bulb extract may be a good source of potential biofuel (mono-alkyl ester), UV A, B and C blockers in the form of diphenyl-ketone, oviposition attractants (long-chain alkanes) and also a source of bioactive molecules such as estragole which shows antispasmodic effect, methyl palmitate with immunosuppressive effect in semi-allografts and hepatoprotective activity in rats, and an analogue of the antidepressant alfetamine.

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