THE IMPROVEMENT OF SEMEN QUALITY AND TESTOSTERONE LEVEL OF BALI CATTLE AFTER PROSTAGLANDIN F2α ADMINISTRATION

This study aimed to determine the effect of prostaglandin F2α (PGF2α) administration on improving semen quality and testosterone levels on Bali cattle. This study used 3 Bali cattle aged 2, 3.5, and 5 years old. The sample used in this study was Bali cattle semen. In this study, the Latin Square Design was used with three different treatments were administered over three time periods. The treatments performed were P1 (5 mL physiological NaCl), P2 (25 mg prostaglandin), and P3 (37.5 mg prostaglandin) while treatment period was three weeks of treatment administration, which were 1st. 2nd, and 3rd week. Blood collected through coccygea vein 30 minutes after treatment to measure testosterone levels using enzyme-linked immunosorbent assay (ELISA) technique. Statistical analysis showed that PGF2α administration at a dose of 25 mg and PGF2α at a dose of 37.5 mg had no effect (P0.05) on volume, color, pH, consistency, concentration, and motility of Bali cattle spermatozoa. The volume of semen obtained from P1; P2; and P3 were 6.5±0.9; 6.3±0.6; and 6.2±1.2 mL, respectively. The color of the semen in P1, P2, and P3 were beige and milky white. The pH in groups P1; P2; and P3 were 6.7±0.6; 7.1±0.2; and 6.5±0.2, respectively. Spermatozoa concentration in P1; P2; and P3 were 1,328±96.43 x 106 cells/mL; 1,354±102.19 x 106 cells/mL; and 1,353.3±88.55 x 106 cells/mL, respectively. Spermatozoa motility in P1; P2; and P3 were 73.3±2.8%; 71.6±2.8%; and 73.3±2.8%, respectively. Testosterone levels in P1 and P3 were 5.05±0.22 and 6.74±1.38 ng/mL, respectively (P0.05). Based on the results of this study, it was concluded that the administration of PGF2α does not affect semen quality but can increase the level of testosterone on Bali cattle.

Download Full-text

D-Dimer Determination to Exclude Pulmonary Embolism: a Two-Step Approach Using Latex Assay as a Screening Tool

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648817 ◽

1994 ◽

Vol 72 (01) ◽

pp. 089-091 ◽

Cited By ~ 21

Author(s):

P de Moerloose ◽

Ph Minazio ◽

G Reber ◽

A Perrier ◽

H Bounameaux

Keyword(s):

Pulmonary Embolism ◽

Screening Tool ◽

Screening Tests ◽

Enzyme Linked Immunosorbent Assay ◽

Elisa Assay ◽

Elisa Technique ◽

Diagnostic Step ◽

D Dimer ◽

Rule Out ◽

Latex Test

SummaryD-dimer (DD), when measured by a quantitative enzyme-linked immunosorbent assay (ELISA), is a valuable test to exclude venous thromboembolism (VTE). However, DD ELISA technique is not appropriate for emergency use and the available agglutination latex assays are not sensitive enough to be used as an alternative to rule out the diagnosis of VTE. Latex assays could still be used as screening tests. We tested this hypothesis by comparing DD levels measured by ELISA and latex assays in 334 patients suspected of pulmonary embolism. All but one patient with a positive (DD ≥500 ng/ml) latex assay had DD levels higher than 500 ng/ml with the ELISA assay. Accordingly, ELISA technique could be restricted to patients with a negative result in latex assay. This two-step approach would have spared about 50% of ELISA in our cohort. In conclusion, our data indicate that a latex test can be used as a first diagnostic step to rule out pulmonary embolism provided a negative result is confirmed by ELISA and the performance of the latex assay used has been assessed properly.

Download Full-text

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Czech Journal of Animal Science ◽

10.17221/79/2017-cjas ◽

2018 ◽

Vol 63 (No. 4) ◽

pp. 127-135 ◽

Cited By ~ 1

Author(s):

T. Slanina ◽

M. Miškeje ◽

F. Tirpák ◽

M. Błaszczyk ◽

R. Stawarz ◽

...

Keyword(s):

Meleagris Gallopavo ◽

Lower Percentage ◽

Computer Assisted ◽

Viability Assessment ◽

Spermatozoa Motility ◽

In Vitro Incubation ◽

Time Periods ◽

Casa System ◽

Motility Parameters

The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine – from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87–9.90%) if compared to 5°C (2.12–4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.

Download Full-text

Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822012000400019 ◽

2012 ◽

Vol 45 (4) ◽

pp. 510-513 ◽

Cited By ~ 17

Author(s):

Teiliane Rodrigues Carneiro ◽

Marta Cristhiany Cunha Pinheiro ◽

Sara Menezes de Oliveira ◽

Ana Lúcia de Paula Hanemann ◽

José Ajax Nogueira Queiroz ◽

...

Keyword(s):

Serological Test ◽

Laboratory Diagnosis ◽

Elisa Test ◽

Northeastern Brazil ◽

Enzyme Linked Immunosorbent Assay ◽

Serological Tests ◽

Predictive Values ◽

Transmission Area ◽

Elisa Technique ◽

Endemic Areas

INTRODUCTION: The laboratory diagnosis of schistosomiasis is based mainly on the detection of parasite eggs in stool samples through the Kato-Katz (KK) technique, reading one slide by test. However, a widely known limitation of parasitological methods is reduced sensitivity, particularly in low endemic areas. METHODS: To increase sensitivity, we conducted further slide readings from the same stool sample using the parasitological method associated with a serological test. We used the KK method (three slides) and the IgG anti-Schistosoma mansoni-enzyme-linked immunosorbent assay (ELISA) technique to diagnose schistosomiasis in low endemic areas in the Brazilian State of Ceará. Fecal samples and sera from 250 individuals were analyzed. RESULTS: Sixteen percent and 47.2% of samples were positive in parasitological tests and serological tests, respectively. Parasitological methods showed that 32 (80%) individuals tested positive on the first slide, 6 (15%) on the second slide, and 2 (5%) on the third. The performance of the ELISA test in the diagnosis, using the KK method as diagnostic reference, showed a negative predictive value of 100%, with specificity and positive predictive values of 62.8% and 33.9%, respectively. CONCLUSIONS: In this study, the increase from one to three slides analyzed per sample using the KK technique was shown to be a useful procedure for increasing the diagnostic sensitivity of this technique.

Download Full-text

PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus

el–Hayah ◽

10.18860/elha.v5i4.3476 ◽

2016 ◽

Vol 5 (4) ◽

pp. 169

Author(s):

Sri Puji Astuti Wahyuningsih ◽

Virid Gibson ◽

Alfiah Hayati

Keyword(s):

Molecular Weight ◽

Treatment Group ◽

Mus Musculus ◽

Control Group ◽

Protein Profiles ◽

Testosterone Levels ◽

Randomized Design ◽

Sds Page ◽

Elisa Technique ◽

Completely Randomized Design

The purpose of this research was to determine the effect of polysaccharide krestin (PSK) on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of 15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of 60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.

Download Full-text

Comparative study of semen quality traits between Etawah grade and Senduro bucks

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/888/1/012022 ◽

2021 ◽

Vol 888 (1) ◽

pp. 012022

Author(s):

N Isnaini ◽

A A Hakim ◽

D Amertaningtyas ◽

H E Sulistyo ◽

A Irsyammawati ◽

...

Keyword(s):

Comparative Study ◽

Artificial Insemination ◽

Recovery Rate ◽

Semen Quality ◽

T Test ◽

Quality Traits ◽

Spermatozoa Motility ◽

Frozen Semen ◽

Semen Volume

Abstract This study compared the semen quality traits between Etawah grade (EGB) and Senduro bucks (SB) raised in Singosari National Artificial Insemination Center, Indonesia. A total of 96 ejaculated semen were collected from three EGB and three SB with an average age of three years. The ejaculates were evaluated for semen volume (SV), spermatozoa concentration (SC), total spermatozoa (TS), spermatozoa motility (SM), post-thawing spermatozoa motility (PTSM), the recovery rate of spermatozoa motility (RRSM), and frozen semen production (FSP). Data of SV, SM, PTSM, and RRSM were compared using the Mann-Whitney U test. While the data of SC, TS, and FSP were analyzed using an independent t-test. The results showed that SV, SM, PTSM, and RRSM were not significantly differed (P>0.05) between breeds. Whereas, SC, TS, and FSP of EGB were significantly higher (P<0.05) than SB. Therefore, it can be concluded that Etawah grade bucks can produce higher total spermatozoa and as a result higher frozen semen production for artificial insemination as compared to Senduro bucks.

Download Full-text

Seroepidemiology of rinderpest in bovids in Sri Lanka using the enzyme linked immunosorbent assay (ELISA) technique

Preventive Veterinary Medicine ◽

10.1016/s0167-5877(98)00106-8 ◽

1998 ◽

Vol 37 (1-4) ◽

pp. 69-75 ◽

Cited By ~ 4

Author(s):

Indira Silva ◽

Ashoka Dangolla ◽

John Allen

Keyword(s):

Sri Lanka ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Elisa Technique

Download Full-text

Angiography significantly decreased serum levels of IL-8 independent of artery stenosis

10.21203/rs.3.rs-119094/v1 ◽

2020 ◽

Author(s):

Lida Zare ◽

Akram Eidi ◽

Mohammad Safarian ◽

Mohammad Kazemi Arababadi

Keyword(s):

Protective Factor ◽

Serum Levels ◽

Artery Stenosis ◽

Enzyme Linked Immunosorbent Assay ◽

Dependent Manner ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Before And After ◽

Elisa Technique ◽

Factor Alpha

Abstract Background Angiography is a safe cardiovascular technique for the diagnosis and treatment of the cardiovascular disorders. The potential effects of angiography on the cytokines are yet to be clarified completely. Interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-α) are the important pro-inflammatory cytokines that participate in the pathogenesis of artery stenosis. The aim of his project was to study the angiography effects on the serum levels of IL-8 and TNF-α. Methods Fifty-five participants in three groups, without, with one and with more than one artery stenosis, were explored in this project. Serum levels of IL-8 and TNF-α were measured in the participants before and after angiography using enzyme linked immunosorbent assay (ELISA) technique. Results Serum levels of IL-8, but not TNF-α, were significantly decreased following angiography. X-ray doses had moderate positive correlation with serum levels of TNF-α in the patients with more than one artery stenosis. Serum levels of IL-8 and TNF-α were not different among male and female participants in all groups. Discussion Angiography may be a protective factor for inflammation in IL-8 dependent manner. Using angiography in the patients with more than one artery stenosis needs to be executed cautiously.

Download Full-text

Preservation of bovine semen using egg yolk substituted with extract guava in water from yellow coconut on the semen quality and fertility of Bali cattle

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/454/1/012071 ◽

2020 ◽

Vol 454 ◽

pp. 012071

Author(s):

A Marawali ◽

M S Abdullah ◽

Jalaludin ◽

W M Nalley ◽

T M Hine ◽

...

Keyword(s):

Semen Quality ◽

Egg Yolk ◽

Bovine Semen ◽

Bali Cattle

Download Full-text

Intrathecal synthesis of soluble HLA-G and HLA-I molecules are reciprocally associated to clinical and MRI activity in patients with multiple sclerosis

Multiple Sclerosis Journal ◽

10.1191/1352458506ms1241oa ◽

2006 ◽

Vol 12 (1) ◽

pp. 2-12 ◽

Cited By ~ 37

Author(s):

E Fainardi ◽

R Rizzo ◽

L Melchiorri ◽

M Castellazzi ◽

E Paolino ◽

...

Keyword(s):

Multiple Sclerosis ◽

Disease Activity ◽

Neurological Disorders ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Concentrations ◽

Intrathecal Synthesis ◽

Soluble Hla ◽

Elisa Technique ◽

Magnetic Resonance Imaging Mri

The aim of this study was to provide further insight into the effective contribution of classical soluble HLA-A, B and C class Ia (sHLA-I) and non-classical soluble HLA-G class Ib (sHLA-G) molecules in immune dysregulation occurring in multiple sclerosis (MS). We evaluated by enzyme-linked immunosorbent assay (ELISA) technique intrathecal synthesis and cerebrospinal fluid (CSF) and serum levels of sHLA-I and sHLA-G in 69 relapsing-remitting (RR), 21 secondary progressive (SP) and 13 primary progressive (PP) MS patients stratified according to clinical and magnetic resonance imaging (MRI) evidence of disease activity. We also tested, as neurological controls, 91 patients with other inflammatory neurological disorders (OIND) and 92 with non-inflammatory neurological disorders (NIND). Eighty-two healthy volunteers served as further controls for sHLA-I and sHLA-G determinations. An intrathecal production of sHLA-I and sHLA-G detected by specific indexes was significantly more frequent in MS patients than in controls (p<0.01). An intrathecal synthesis of sHLA-I was prevalent in clinically (p<0.02) and MRI active (p<0.001) MS, whereas a CSF-restricted release of sHLA-G predominated in clinically (p<0.01) and MRI stable (p<0.001) MS. sHLA-I levels were low in the serum of clinically active (p<0.001) and high in the CSF of MRI active (p<0.01) MS. Conversely, sHLA-G concentrations were decreased in the serum of clinically stable MS (p<0.01) and increased in the CSF of MRI inactive MS (p<0.001). The trends towards a negative correlation observed between CSF and serum concentrations and intrathecal synthesis of sHLA-I and sHLA-G in patients without evidence of clinical and MRI activity confirmed that intrathecal production and fluctuations in CSF and serum concentrations of sHLA-I and sHLA-G were reciprocal in MS. Our results suggest that, in MS, a balance between classical sHLA-I and non-classical sHLA-G products modulating both MRI and clinical disease activity in opposite directions may exist.

Download Full-text