scholarly journals Effect of Temperature Increase on Hemoglobin Concentration and Hematocrit Value of Tilapia Fish (Oreochromis niloticus)

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Zuhrawati N. A.
Keyword(s):  
Oreochromis Niloticus ◽  
Temperature Increase ◽  
Hemoglobin Concentration ◽  
Effect Of Temperature ◽  
Control Group ◽  
Blood Samples ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Fish Hemoglobin ◽  
Banda Aceh

This research aims to determine the effect of temperature increase on hemoglobin consentration and hematocrit value of tilapia fish. This research was conducted at Faculty of Veterinary Medicine Syiah Kuala University Banda Aceh. Samples used were blood from tilapia fish thatderived from 12 tilapia fish with weight range of 40-50 g. The research design used was a completely randomized design (CRD) unidirectionalpattern with 3 treatments and 4 replications. P1 as a control group was the treatment with aquarium temperature of 29±1C, P2 was thetreatment with aquarium temperature of 32±1o C, and P3 was the treatment with aquarium temperature of 35±1o C. All aquarium size in thisstudy was 80 cm x 60 cm x 40 cm with the height of water 30 cm. The treatment was carried out for 15 days and on day 16 blood samples werecollected. Blood was taken through the caudalis vein. Data were analyzed by analysis of variance (ANOVA) one way, followed by Tukey test.Mean (± SD) of tilpia fish hemoglobin levels (g/dl) on P1, P2, and P3 were 4.50±0.49, 5.08±0.79, and 6.08±0.94, respectively. Mean (± SD) oftilapia fish hematocrit value (%) in P1, P2, and P3 consecutively were 26.50±8.10, 33.25±11.78, and 40.25±9.04. Statistical analysis showed thatan increase in temperature showed no effect (P 0.05) to the hemoglobin levels of tilapia fish and highly significant to the hematocrit value (P0.01). It can be concluded that the increase of water temperature on 32±1° C and 35±1° C in can increase the hemoglobin levels andhematocrit value of tilapia fish.____________________________________________________________________________________________________________________Key words: tilaphia fish, hemoglobin levels, hematocri value, temperature o

scholarly journals Sublethal toxicity test of mercury (Hg) in the flesh and tissue of Tilapia (Oreochromis niloticus)

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (2) ◽  
pp. 99
Author(s):  
Yusnaini Yusnaini ◽  
Emiyarti Emiyarti ◽  
Indriyani Nur ◽  
Oce Astuti ◽  
Rahmad Sofyan Patadjai
Keyword(s):  
Tissue Damage ◽  
Oreochromis Niloticus ◽  
Toxicity Test ◽  
Control Group ◽  
Sublethal Toxicity ◽  
Mononuclear Cell Infiltration ◽  
Tubular Necrosis ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hg Accumulation

This study tested the sublethal toxicity of mercury (Hg) in the flesh and tissue of tilapia (Oreochromis niloticus). This research used an experimental method completely randomized design (CRD) with 3 treatments and 3 replications. The treatments used different Hg concentrations: Treatment A = 0.08 ppm; B = 0.16 ppm; C = 0.24 ppm; and the control group = without the addition of Hg. The parameters observed were the accumulation of mercury (Hg) in the flesh and tissue damage (histopathology) of the gills, kidneys and liver of tilapia. The results showed that the highest Hg accumulation was at the highest Hg concentration (treatment C = 0.24 ppm). Tissue damage was mainly found in the kidneys, liver and gills of fish exposed to Hg with indications of bleeding, tubular necrosis, vacuolization of epithelial cells, and mononuclear cell infiltration. The results showed that the toxicity of mercury to the organs increased with the increase in the concentration of Hg in water. Keywords : flesh, mercury, Oreochromis niloticus, sublethal, tissue

scholarly journals Masculinization of Tilapia (Oreochromis niloticus) Using Methyl Testosterone Natural Hormone

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Rosmaidar R ◽  
Winaruddin W ◽  
Mimi Herlina
Keyword(s):  
Oreochromis Niloticus ◽  
Fish Larvae ◽  
Hormone Treatment ◽  
Control Fish ◽  
Control Group ◽  
Group Iii ◽  
Randomized Design ◽  
Natural Hormone ◽  
Male Sex ◽  
Completely Randomized Design

This study aimed to determine the effect of methyl testosterone natural hormoneon the rate of masculinization of tilapia (Oreochromis niloticus). The study used 270 tilapia larvae with the age of 7 days as experimental animals. Tilapia larvae were divided into 3 treatments; eachtreatment consisted of 30 larvae fish. This study was an experimental study using completely randomized design with 3 treatments and 3replications. Treatment I as control group was fed with pellets ad libitum, treatment II was given feed containing methyl testosterone naturalhormone, and treatment group III was given hormone treatment by soaking the fish larvae with methyl testosterone natural hormone for 20hours. Larvae were raised in accordance with applicable procedures. Observation was conducted when the larvae fish were at 60 days old andthe sex of fish can be observed. Parameter examined was the percentage of the male sex. The average (± SD) percentage of male tilapia in groupPI, PII, and PIII were 36.39±3.97, 66.72±6.13, and 82.37±6.87%, respectively. The results showed that the percentage of male sex of tilapia fedwith natural hormone of methyl testosterone through the diet and dipping was significantly different (P0.05) from control fish. In conclusion,the administration of natural hormone of methyl testosterone increased the percentage of the male sex of tilapia).____________________________________________________________________________________________________________________Key words: tilapia larvae, natural hormone methyl testosterone, the male sex percentage

scholarly journals Hematological response of Tilapia (Oreochromis niloticus) in Laundry wastewater

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Saparuddin Saparuddin ◽  
Yanti Yanti ◽  
Salim Salim ◽  
Harish Muhammad
Keyword(s):  
Oreochromis Niloticus ◽  
Physiological Condition ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Hemoglobin Level ◽  
Blood Components ◽  
High Concentration ◽  
A Value ◽  
Randomized Design ◽  
Completely Randomized Design

The high concentration of detergent in the aquatic ecosystem potentially affects the physiological condition of the fish by disrupting the respiration process and changing the concentration of blood components and chemistry. The purpose of this study was to determine the condition of the hematological parameters of tilapia (Oreochromis niloticus) exposed to wastewater from the laundry industry. Each treatment was stocked with five fish per aquarium (50 x 30 x 30 cm). This study used a completely randomized design (CRD) technique with six treatments and three replications: P0 (0%) as a control, P1 (1%), P2 (2%), P3 (3%), P4 (4%) and P5 (5%) with each treatment exposed to a specific concentration of wastewater and residues. The results showed that the hemoglobin levels of treatments were decreasing, with the lowest mean of hemoglobin level found in the P2 (7.05 gr%), and the lowest concentration on the 30th day was 7.71 gr%. There were no significant effects of wastewater to the number of erythrocytes and leucocytes among treatments (P>0.05). While there were increasing levels of hematocrit, the largest mean level was found in the P4 treatment with a value of 24.11%, and the largest mean on the 20th day of observation showed a value of 23.51%. Wastewater from the laundry industry can affect the hematological condition of tilapia by decreasing the hemoglobin concentration and increasing the hematocrit levels above the normal condition.

scholarly journals EFEK PENINGKATAN SUHU TERHADAP JUMLAH LEUKOSIT IKAN NILA (Oreochromis niloticus) (The Effect of Temperature Increase on Leukocyte Count of Nile Tilapia (Oreochromis niloticus))

2016 ◽  
Vol 10 (1) ◽  
pp. 31
Author(s):  
Nauval Gibran Lubis ◽  
Sugito Sugito ◽  
Zuhrawati Zuhrawati ◽  
Zuraidawati Zuraidawati ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Water Temperature ◽  
Oreochromis Niloticus ◽  
Temperature Increase ◽  
Nile Tilapia ◽  
Leucocyte Count ◽  
Effect Of Temperature ◽  
Total Leucocyte Count ◽  
Randomized Design ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Range Test

The aim of this research was to find out the effect of water temperature increase on leucocyte count of nile tilapia. The blood sample from 9 tilapia with weigh of 40-50 g were used in this study. The research was conducted using completely randomized design (CRD) with 3 treatments and 3 replications. Treatment P1 as a control with aquarium temperature was maintained at 29±1 C. Water temperature in treatment P2 and P3 was maintained at 32±1 C and 35±1 C respectively. The aquarium size in each treatment group was 80x60x40 cm with height of water 30 cm. The treatment was done for 15 days and on day 16th, the blood were collected from caudal vein. Data were analyzed with analysis of variance (Anova) and followed with Duncan's multiple range test. The result showed that total leucocyte count (x 105/mm3) of nile tilapia in group P1, P2, and P3 were 3.08±0.88, 1.70±0.52, and 1.69±0.20 respectively. lts showed that an increase in temperature significantly affect (P<0.05) the leucocyte count of nile tilapia. The conclusion of this research is the increase in water temperature in aquarium decrease the leucocyte count of nile tilapia.

scholarly journals Eksplorasi Rizobakteri Indigenous Dan Uji Antagonis Terhadap Patogen Rigidoporus microporus Dan Phellinus noxius Pada Tanaman Karet (Hevea brasiliensis) Secara In Vitro

2020 ◽  
Vol 3 (4) ◽  
pp. 43-52
Author(s):  
Murtadha Murtadha ◽  
M. Abduh Ulim ◽  
Syamsuddin Syamsuddin
Keyword(s):  
Plant Growth ◽  
Hevea Brasiliensis ◽  
Growth Promoter ◽  
Randomized Design ◽  
Pathogen Control ◽  
Completely Randomized Design ◽  
Experimental Stage ◽  
Phellinus Noxius ◽  
Banda Aceh

Abstrak.  Penelitian bertujuaan untuk mendapatkan isolat rizobakteri yang mampu berperan sebagai agens biokontrol terhadap pengendalian patogen R. microporus dan P. noxius secara in vitro serta sebagai agen rizobakteri pemacu pertumbuhan tanaman (RPPT). Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh, mulai Oktober sampai Desember 2017. Penelitian ini menggunakan Rancangan Acak Lengkap non faktorial. Faktor yang diteliti yaitu isolat rizobakteri, taraf yang dicobakan terdiri dari 15 isolat dan dua patogen antagonis R. microporus dan P. noxius, yang diulang sebanyak 3 kali sehingga terdapat 90 unit satuan percobaan. Hasil penelitian menunujukkan isolat rizobakteri mampu menekan pertumbuhan koloni cendawan patogen. Pada patogen uji R. microporus terdapat 3 rizobakteri yang paling baik yaitu isolat DLG5/3 dengan persentase penghambatan 68,33%, DLG4/1 dengan persentase penghambatan 66,66% dan DLG4/7 dengan persentase penghambatan 63,33%. Pada patogen uji P.noxius terdapat dua rizobakteri yang paling baik dalam menghambat pertumbuhan koloni cendawan patogen yaitu isolat DLG5/1 dengan persentase penghambatan 60,33%, dan DKP6/3 dengan persentase penghambatan 52,50%. Pada laju penghambatan isolat rizobakteri yang paling baik pada patogen R. microporus yaitu isolat DLG6/4 dan DKP4/1 dengan nilai rerata laju penghambatan 20,33 mm/hari. Pada patogen P. noxius menunjukan isolat rizobakteri yang paling baik yaitu isolat DLG4/1 dengan nilai laju penghambatan 12,05 mm/hari.Exploration of Indigenous Rizobacteria and Antagonistic Test against Patogen Rigidoporus microporus And Phellinus noxius In Rubber Plant (Hevea brasiliensis) In VitroAbstract. The research was conducted to obtain rhizobacteria isolates capable of acting as biocontrol agents on pathogen control of R. microporus and P. noxius in vitro and as plant growth promoter rhizobacteria (PGPR). The research was conducted at the Science and Technology of seed Laboratory, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh, starts from October to December 2017. The research using Completely Randomized Design non factorial. Factors researched were rhizobacteria isolates, the experimental stage consisted of 15 isolates and 2 antagonist were R. microporus dan P. noxius, and 3 time repeated until be found 90 units of treatment. The results showed that rhizobacteria isolates capable to inhibith growth of colonies pathogenic. In the pathogen of R. microporus test, there are 3 better rhizobacteria were DLG5/3 isolate with 68.33% inhibition percentage, DLG4/1 with 66.66% inhibition percentage and DLG4/7 with 63,33% inhibition percentage. In the pathogen of P. noxius there are 2 best rhizobacteria inhibiting growth, DLG5/1 isolate with 60.33% inhibition percentage, and DKP6/3 with 52,50% inhibition percentage. In the pathogen of R. microporus test, there are 2 better rhizobacteria were DLG6/4 and DKP4/1 with 20,33 mm/day inhibition percentage. In the pathogen of P. noxius test,  the best rhizobacteria were DLG4/1  with 12,05 mm/day inhibition percentage. 

scholarly journals The Effect Of Rajalele Probiotic On Survival Rate Of Fish Juvenile Pangasius pangasius, Oreochromis niloticus, Cyprinus carpio

2020 ◽  
Vol 6 (1) ◽  
pp. 88-95
Author(s):  
Marlina Doloksaribu ◽  
Robert Martua Simanjuntak ◽  
Ied Hidayani Parinduri
Keyword(s):  
Social Sciences ◽  
Experimental Study ◽  
Data Analysis ◽  
Survival Rate ◽  
Cyprinus Carpio ◽  
Oreochromis Niloticus ◽  
Fish Farming ◽  
Randomized Design ◽  
The Social ◽  
Completely Randomized Design

The use of probiotics is certainly one of the solutions in overcoming the problem of increasing seed in fish farming. One of them is the use of probiotic king catfish, in this study tested on catfish, tilapia and goldfish. This research is an experimental study. The design used in this study was a Completely Randomized Design with 4 treatments 3 replications. Data analysis used Statistical Package for the Social Sciences version 15. The results of the analysis of the highest seed survival rate were obtained on Pangasius pangasius (95%), on Oreochromis niloticus (83,33 %), and Cyprinus carpio (80%). The treatment of Rajalele probiotics has a very significant effect (very significant) Fanalysis (23.01) > Ftable 0.01 (7.59) on the graduation of Pangasius pangasius, Oreochromis niloticus, and Cyprinus carpio. The conclusion of this study shows that the survival rate the highest in Pangasius pangasius

scholarly journals PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 169
Author(s):  
Sri Puji Astuti Wahyuningsih ◽  
Virid Gibson ◽  
Alfiah Hayati
Keyword(s):  
Molecular Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Control Group ◽  
Protein Profiles ◽  
Testosterone Levels ◽  
Randomized Design ◽  
Sds Page ◽  
Elisa Technique ◽  
Completely Randomized Design

<em>The purpose of this research was to determine the effect of polysaccharide krestin</em> (<em>PSK) </em><em>on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of  15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of  60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.</em>

scholarly journals PENGARUH PEMBERIAN PROBIOTIK Bacillus spp. MELALUI PAKAN TERHADAP KELANGSUNGAN HIDUP DAN LAJU PERTUMBUHAN IKAN NILA (Oreochromis niloticus)

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Muhammad Masyarul Rusdani, Sadikin Amir Saptono Waspodo dan Zaenal Abidin
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Oreochromis Niloticus ◽  
Specific Growth ◽  
Good Combination ◽  
Randomized Design ◽  
Bacillus Spp ◽  
Completely Randomized Design ◽  
Total Bacteria ◽  
Different Doses

ABSTRAKPenelitian ini bertujuan untuk menguji kinerja bakteri probiotik Bacillus spp. dan prebiotik molase dengan dosis berbeda terhadap tingkat kelangsungan hidup dan kinerja pertumbuhan ikan nila (Oreochromis niloticus). Penelitian ini menggunakan motede eksperimen dengan rancangan acak lengkap (RAL) dan melibatkan 5 perlakuan, yaitu kontrol (K), Bacillus spp. 10 ml/kg pakan (A); Bacillus spp. 10 ml/Kg pakan + molase 10 v/v (B); Bacillus spp. 20 ml/Kg pakan (C); dan Bacillus spp. 20 ml/Kg pakan + molase 10 v/v(D). Hasil yang diperoleh menunjukkan variasi untuk total bakteri, yaitu dari 2,24x108 hingga 3,78x108 CFU/ml. Demikian juga dengan total Bacillus spp. dari 1,07x106 hingga 2,02x107 CFU/ml. Adapun untuk pengamatan tingkat kelangsungan hidup dan laju pertumbuhan tidak menunjukkan hasil yang signifikan. Meski demikian, perlakuan D memiliki hasil yang relatif lebih baik dengan nilai SR dan LPH berturut-turut 96,67% dan 1,01 %/hari. Kata kunci : probiotik Bacillus spp., prebiotik molase,  total bakteri dan Bacillus spp., tingkat kelangsungan hidup, laju pertumbuhan harian  ABSTRACTThis study was aimed to determine the good combination of probiotic bacillus spp. and prebiotic molasses with different doses to survival rate and growth performance of tilapia (Oreochromis niloticus). this study has used experimental design with completely randomized design (CRD) and five treatment, i.e. Control (K), without probiotic and prebiotic; Bacillus spp. 10 ml/kg feed (A); Bacillus spp. 10 ml/Kg feed + molasses 10 v/v (B); Bacillus spp. 20 ml/Kg feed (C); dan Bacillus spp. 20 ml/Kg feed + molasses 10 v/v (D). The results showed variation for total bacteria and total Bacillus spp. The average of total bacteria obtained in this study ranged from 2,24x108 to 3.78x108 CFU/ml. Likewise, the total Bacillus spp. were obtained from 1.07x106 to 2.02x107 CFU/ml. While for survival rate (SR) and the growth rate (DGR) did not showed significant results. However, treatment D has a relatively better then treatment else with SR and DGR respectively 96.67% and 1.01%.day-1. Keywords : probiotic Bacillus spp., prebiotic molasses, total bacteria and Bacillus spp., survival rate, specific growth rate.

scholarly journals Pengaruh Suplementasi Probiotik A3-51 terhadap Derajat Imunitas Oreochromis niloticus Didasarkan pada Angka Kuman pada Ginjal Setelah Uji Tantang dengan Aeromonas hydrophila dan Aeromonas salmonicida achromogenes

2006 ◽  
Vol 8 (2) ◽  
pp. 144
Author(s):  
Agus Irianto ◽  
Hernayanti Hernayanti ◽  
Ning Iriyanti
Keyword(s):  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Aeromonas Salmonicida ◽  
Control Fish ◽  
System Response ◽  
Randomized Design ◽  
Immune System Response ◽  
Completely Randomized Design ◽  
Number Of Bacteria

Objectives of this research was to know the effect of probiotic A3-51 supplementation on food to the total number of bacteria in kidney and mortality of Oreochromis niloticus after challenge test with Aeromonas hydrophila and Aeromonas salmonicida achromogenes. This research was consisted of two experiment, the first was to know the highest immunity level of fish based on the number of macrophage and its phagocytic activity. Meanwhile, the second was to know the immunity level of fish challenged with A. hydrophila and A. salmonicida achromogenes by intra-peritoneal injection. The experiment used Completely Randomized Design in triplicates. The result from the first experiment showed that the highest non spesific immune system response, by means number of macrophage, was found 21 days treatment. The second experiment showed that the highest total number of bacteria in kidney and mortality level were 20.23 x 108 cells/g and 46.67%, respectively, both was found in control fish injected intra-peritoneally with A. salmonicida achromogenes.

scholarly journals PENGGUNAAN SLUDGE SEBAGAI PAKAN CALON INDUK IKAN NILA MERAH (Oreochromis sp.)

2003 ◽  
Vol 5 (2) ◽  
pp. 11
Author(s):  
Rustidja Rustidja
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Specific Growth ◽  
Control Group ◽  
Fish Feed ◽  
Commercial Fish ◽  
Gonadal Maturation ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Maturation Index

The aim of study was to know the effect of sludge in the ration on the gonadal growth of Oreochromis sp. broodstock. Experimental design used in this experiment was completely Randomized Design. The broodstocks were fed with the ration containing 0-40% of sludge, or with a commercial fish feed as a control.The results were not significantly differences between the treatment in all parameters. The sludge in ration ranging from 0 to 40% resulted the level of gonadal maturation of 4, 6, 7, dan 9. The value of Gonadal Maturation Index on 0,96 to 3,98 % and the control group on 1,75 %. The specific growth rate of broodstock fed with the ration ranged from 1,1 to 1,62 Bw/day and that of the development of gonadal control group was 1,97. The survival rate were variated between 14,29 to 100% and that of the development of gonadal control group is 71,43%. The ration containing 40% sludge is the most suitable feed for maturation in Oreochromis.

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