scholarly journals Pengaruh Suplementasi Probiotik A3-51 terhadap Derajat Imunitas Oreochromis niloticus Didasarkan pada Angka Kuman pada Ginjal Setelah Uji Tantang dengan Aeromonas hydrophila dan Aeromonas salmonicida achromogenes

2006 ◽  
Vol 8 (2) ◽  
pp. 144
Author(s):  
Agus Irianto ◽  
Hernayanti Hernayanti ◽  
Ning Iriyanti
Keyword(s):  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Aeromonas Salmonicida ◽  
Control Fish ◽  
System Response ◽  
Randomized Design ◽  
Immune System Response ◽  
Completely Randomized Design ◽  
Number Of Bacteria

Objectives of this research was to know the effect of probiotic A3-51 supplementation on food to the total number of bacteria in kidney and mortality of Oreochromis niloticus after challenge test with Aeromonas hydrophila and Aeromonas salmonicida achromogenes. This research was consisted of two experiment, the first was to know the highest immunity level of fish based on the number of macrophage and its phagocytic activity. Meanwhile, the second was to know the immunity level of fish challenged with A. hydrophila and A. salmonicida achromogenes by intra-peritoneal injection. The experiment used Completely Randomized Design in triplicates. The result from the first experiment showed that the highest non spesific immune system response, by means number of macrophage, was found 21 days treatment. The second experiment showed that the highest total number of bacteria in kidney and mortality level were 20.23 x 108 cells/g and 46.67%, respectively, both was found in control fish injected intra-peritoneally with A. salmonicida achromogenes.

POTENSI TUMBUHAN BANGKAL (Nauclea Orientalis) UNTUK PENGENDALIAN BAKTERI Aeromonas Hydrophila (POTENTIAL PLANT BANGKAL (Nauclea orientalis) FOR CONTROL Aeromonas Hydrophila)

2016 ◽  
Vol 2 (4) ◽  
pp. 166
Author(s):  
Siti Aisiah
Keyword(s):  
Aeromonas Hydrophila ◽  
Fish Farming ◽  
Quality Parameters ◽  
Control Fish ◽  
Toxicity Tests ◽  
Distilled Water ◽  
Normal Range ◽  
Water Solvent ◽  
Randomized Design ◽  
Completely Randomized Design

Tujuan penelitian ini adalah didapatkan metode pengendalian penyakit yang disebabkan oleh bakteri Aeromonas hydrophila pada budi daya ikan yang ramah lingkungan. Dalam penelitian ini dilakukan uji sensitivitas terhadap bakteri A. hydrophila, uji minimal konsentrasi menghambat  bakteri A. hydrophila (uji MIC) dan uji toksisitas terhadap ikan nila. Rancangan  yang digunakan untuk  uji toksisitas  adalah rancangan acak lengkap (RAL), dengan 4 perlakuan yaitu A  = Ikan disuntik dengan ekstrak bangkal konsentrasi 20%, B  = Ikan disuntik dengan ekstrak bangkal konsentrasi 40%,  C  = Ikan disuntik dengan ekstrak bangkal konsentrasi 80%, dan D  = Kontrol (ikan tidak disuntik), diulang sebanyak 3 kali. Perlakuan ini didapat dari hasil uji sensitivitas antibakteri bangkal yang mempunyai daya hambat dan daya bunuh paling besar terhadap bakteri A. hydrophila yaitu ekstrak daun bangkal dengan pelarut akuades. Pengujian MIC menunjukkan bahwa ekstrak bangkal-akuades memiliki daya hambat minimal  20 % terhadap aktivitas bakteri A. hydrophila. Hasil uji toksisitas yang dilakukan terhadap ikan nila dengan konsentrasi 20%, 40% dan 80%  mortalitas ikan nila dibawah 50 %. Pengamatan hematologis yaitu eretrosit, leokosit, plasma darah, hematokrit dan leokokrit pada masing-masing perlakuan sebagian besar masih berada dalam kisaran yang normal. Parameter kualitas air yaitu, kadar oksigen terlarut,  pH, amoniak, CO2 dan suhu masih dapat mendukung kehidupan normal ikan nila.The purpose of this study was obtained method of controlling diseases caused by Aeromonas hydrophila in fish farming is environmentally friendly. In this study the sensitivity of the test  A. hydrophila, a minimum test konsentari inhibiting A. hydrophila (MIC test) and toxicity test on  tilapia. The design used for toxicity tests is completely randomized design with 4 treatments it A = Fish injected with extracts bangkal concentration of 20 %,  B = Fish injected with extracts bangkal concentration of 40 %, C = Fish injected with extracts of 80 % concentration bangkal, and D = Control ( fish not injected ), repeated 3 times. This treatment was obtained from the results of the sensitivity test antibacterial bangkal inhibition and has the power to kill most of the bacteria against A. hydrophila is bangkal leaf extract with distilled water solvent. MIC testing showed that the extract bangkal - distilled water has a minimum of 20 % inhibition of the bacterial activity A. hydrophila. Results of toxicity tests conducted on tilapia with a concentration of 20 %, 40 % and 80 % mortality of tilapia under 50 %. Haematological observation that eretrosit, leokosit, blood plasm, hematocrit and leokokrit in each treatment is still in the normal range. The water quality parameters, dissolved oxygen, pH, ammonia, CO2 and temperature can still support the normal life of tilapia.

scholarly journals THE EFFECT OF ONION (Allium sativum) EXTRACT TO TREAT TILAPIA FRY (Oreochromis niloticus) WITH BACTERIAL INFECTED Aeromonas hydrophila

2021 ◽  
Vol 1 (1) ◽  
pp. 29-39
Author(s):  
Ani Lestari ◽  
Nurliah ◽  
Baiq Hilda Astriana
Keyword(s):  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Allium Sativum ◽  
Clinical Symptoms ◽  
Survival Rates ◽  
Garlic Extract ◽  
Study Program ◽  
Percent Survival ◽  
Randomized Design ◽  
Completely Randomized Design

Research on the effect of garlic extract (allium sativum) to treat tilapia (oreochromis niloticus) seeds which are infected with aeromonas hydrophila bacteria. This research was conducted for 14 days from 18 December to 31 December 2019 which took place at the Wet Laboratory of the Aquaculture Study Program, Mataram University, West Nusa Tenggara (NTB). This study used a completely randomized design (CRD) with an experimental method consisting of 5 treatments and 3 replications. The container used in this study was a 15 liter jar with a volume of 10 liters. The containers used are 15 units, where in treatment one (P1) using without soaking garlic extract and without being infected with Aeromonas hydrophila bacteria, treatment two (P2) without soaking garlic extract and being infected with Aeromonas hydrophila bacteria, (P3) using soaking onion extract white with a concentration of 1% and infected with Aeromonas hydrophila bacteria, (P4) using garlic extract immersion with a concentration of 1.5% and infected with Aeromonas hydrophila bacteria, (P5) using garlic extract immersion with a concentration of 2% and infected with Aeromonas hydrophila bacteria. The parameters observed during the study included survival rate (SR), relative percent survival (RPS), observation of clinical symptoms, and water quality. The results showed that giving garlic extract with different concentrations had a significantly different effect on survival rates and relative percent survival (RPS).

scholarly journals Masculinization of Tilapia (Oreochromis niloticus) Using Methyl Testosterone Natural Hormone

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Rosmaidar R ◽  
Winaruddin W ◽  
Mimi Herlina
Keyword(s):  
Oreochromis Niloticus ◽  
Fish Larvae ◽  
Hormone Treatment ◽  
Control Fish ◽  
Control Group ◽  
Group Iii ◽  
Randomized Design ◽  
Natural Hormone ◽  
Male Sex ◽  
Completely Randomized Design

This study aimed to determine the effect of methyl testosterone natural hormoneon the rate of masculinization of tilapia (Oreochromis niloticus). The study used 270 tilapia larvae with the age of 7 days as experimental animals. Tilapia larvae were divided into 3 treatments; eachtreatment consisted of 30 larvae fish. This study was an experimental study using completely randomized design with 3 treatments and 3replications. Treatment I as control group was fed with pellets ad libitum, treatment II was given feed containing methyl testosterone naturalhormone, and treatment group III was given hormone treatment by soaking the fish larvae with methyl testosterone natural hormone for 20hours. Larvae were raised in accordance with applicable procedures. Observation was conducted when the larvae fish were at 60 days old andthe sex of fish can be observed. Parameter examined was the percentage of the male sex. The average (± SD) percentage of male tilapia in groupPI, PII, and PIII were 36.39±3.97, 66.72±6.13, and 82.37±6.87%, respectively. The results showed that the percentage of male sex of tilapia fedwith natural hormone of methyl testosterone through the diet and dipping was significantly different (P0.05) from control fish. In conclusion,the administration of natural hormone of methyl testosterone increased the percentage of the male sex of tilapia).____________________________________________________________________________________________________________________Key words: tilapia larvae, natural hormone methyl testosterone, the male sex percentage

scholarly journals EFISIENSI PAKAN, PERTUMBUHAN, KELANGSUNGAN HIDUP DAN RESPON IMUN IKAN PATIN (Pangasius sp.) YANG DIBERI PAKAN BERSINBIOTIK

2018 ◽  
Vol 5 (2) ◽  
pp. 140-154
Author(s):  
Enromauli Pangaribuan ◽  
Ade Dwi Sasanti ◽  
Mohamad Amin
Keyword(s):  
Immune Response ◽  
Aeromonas Hydrophila ◽  
Feed Efficiency ◽  
Clinical Symptoms ◽  
Challenge Test ◽  
Growth And Survival ◽  
Randomized Design ◽  
Commercial Feed ◽  
Completely Randomized Design

ABSTRACTSynbiotic is a combination of probiotics and prebiotics can improve the feed efficiency, growth, survival and BAL population of fish.  The purpose of this research was to know the effect of synbiotic feed to feed efficiency, growth, survival and immune response of catfish. This research conducted on October to December 2016 in Laboratorium Dasar Perikanan, Departement of Budidaya Perairan, Faculty of Agriculture, Sriwijaya University. The method used in the research is Completely Randomized Design (CRD) with six treatments and three replications. Catfish with synbiotic feed that treatment with the addition of commercial feed with synbiotic P0 (Control), P1 (2.5 ml  Prebiotics + Probiotics 6 ml /kg), P2 (5.0 ml  Prebiotics + Probiotics 6 ml /kg), P3 (7.5 ml  Prebiotics + Probiotics 6 ml /kg), P4 (10.5  ml  Prebiotics + Probiotics 6 ml /kg) and P5 (12.5 ml  Prebiotics + Probiotics 6 ml /kg) for 30 days. The observed parameters were feed efficiency, growth, survival, BAL populations in the gut and clinical symptoms after challenge test. The results showed that provision addition of synbiotic in feed give significant effect to feed efficiency, growth and survival of catfish. The treatment P2 with synbiotic feed (5.0 ml Prebiotics + 6 ml Probiotics /kg) gives the feed efficiency (39,27%), the highest growth of weight (4.38 g), the highest growth of length (1.74 cm),  the survival (97.78%), LAB (Lactid Acid Bacteria)  population (3.51 x 104 CFU.mL-1) and survival after test challenge with A. hydrophila (86.67%).  Keywords: Sinbiotic, Pakan, Pangaius sp, Aeromonas hydrophila

scholarly journals The Effect Of Rajalele Probiotic On Survival Rate Of Fish Juvenile Pangasius pangasius, Oreochromis niloticus, Cyprinus carpio

2020 ◽  
Vol 6 (1) ◽  
pp. 88-95
Author(s):  
Marlina Doloksaribu ◽  
Robert Martua Simanjuntak ◽  
Ied Hidayani Parinduri
Keyword(s):  
Social Sciences ◽  
Experimental Study ◽  
Data Analysis ◽  
Survival Rate ◽  
Cyprinus Carpio ◽  
Oreochromis Niloticus ◽  
Fish Farming ◽  
Randomized Design ◽  
The Social ◽  
Completely Randomized Design

The use of probiotics is certainly one of the solutions in overcoming the problem of increasing seed in fish farming. One of them is the use of probiotic king catfish, in this study tested on catfish, tilapia and goldfish. This research is an experimental study. The design used in this study was a Completely Randomized Design with 4 treatments 3 replications. Data analysis used Statistical Package for the Social Sciences version 15. The results of the analysis of the highest seed survival rate were obtained on Pangasius pangasius (95%), on Oreochromis niloticus (83,33 %), and Cyprinus carpio (80%). The treatment of Rajalele probiotics has a very significant effect (very significant) Fanalysis (23.01) > Ftable 0.01 (7.59) on the graduation of Pangasius pangasius, Oreochromis niloticus, and Cyprinus carpio. The conclusion of this study shows that the survival rate the highest in Pangasius pangasius

scholarly journals PENGARUH PEMBERIAN PROBIOTIK Bacillus spp. MELALUI PAKAN TERHADAP KELANGSUNGAN HIDUP DAN LAJU PERTUMBUHAN IKAN NILA (Oreochromis niloticus)

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Muhammad Masyarul Rusdani, Sadikin Amir Saptono Waspodo dan Zaenal Abidin
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Oreochromis Niloticus ◽  
Specific Growth ◽  
Good Combination ◽  
Randomized Design ◽  
Bacillus Spp ◽  
Completely Randomized Design ◽  
Total Bacteria ◽  
Different Doses

ABSTRAKPenelitian ini bertujuan untuk menguji kinerja bakteri probiotik Bacillus spp. dan prebiotik molase dengan dosis berbeda terhadap tingkat kelangsungan hidup dan kinerja pertumbuhan ikan nila (Oreochromis niloticus). Penelitian ini menggunakan motede eksperimen dengan rancangan acak lengkap (RAL) dan melibatkan 5 perlakuan, yaitu kontrol (K), Bacillus spp. 10 ml/kg pakan (A); Bacillus spp. 10 ml/Kg pakan + molase 10 v/v (B); Bacillus spp. 20 ml/Kg pakan (C); dan Bacillus spp. 20 ml/Kg pakan + molase 10 v/v(D). Hasil yang diperoleh menunjukkan variasi untuk total bakteri, yaitu dari 2,24x108 hingga 3,78x108 CFU/ml. Demikian juga dengan total Bacillus spp. dari 1,07x106 hingga 2,02x107 CFU/ml. Adapun untuk pengamatan tingkat kelangsungan hidup dan laju pertumbuhan tidak menunjukkan hasil yang signifikan. Meski demikian, perlakuan D memiliki hasil yang relatif lebih baik dengan nilai SR dan LPH berturut-turut 96,67% dan 1,01 %/hari. Kata kunci : probiotik Bacillus spp., prebiotik molase,  total bakteri dan Bacillus spp., tingkat kelangsungan hidup, laju pertumbuhan harian  ABSTRACTThis study was aimed to determine the good combination of probiotic bacillus spp. and prebiotic molasses with different doses to survival rate and growth performance of tilapia (Oreochromis niloticus). this study has used experimental design with completely randomized design (CRD) and five treatment, i.e. Control (K), without probiotic and prebiotic; Bacillus spp. 10 ml/kg feed (A); Bacillus spp. 10 ml/Kg feed + molasses 10 v/v (B); Bacillus spp. 20 ml/Kg feed (C); dan Bacillus spp. 20 ml/Kg feed + molasses 10 v/v (D). The results showed variation for total bacteria and total Bacillus spp. The average of total bacteria obtained in this study ranged from 2,24x108 to 3.78x108 CFU/ml. Likewise, the total Bacillus spp. were obtained from 1.07x106 to 2.02x107 CFU/ml. While for survival rate (SR) and the growth rate (DGR) did not showed significant results. However, treatment D has a relatively better then treatment else with SR and DGR respectively 96.67% and 1.01%.day-1. Keywords : probiotic Bacillus spp., prebiotic molasses, total bacteria and Bacillus spp., survival rate, specific growth rate.

scholarly journals Kandungan Total Bakteri dan Total Fungi pada Pellet Limbah Penetasan yang Dibuat dengan Penambahan Bentonit

2016 ◽  
Vol 16 (2) ◽  
pp. 69
Author(s):  
Aprilian Adi Nugroho ◽  
Sri Sumarsih ◽  
Bambang Sulistiyanto
Keyword(s):  
Analysis Of Variance ◽  
Total Content ◽  
Duncan Multiple Range Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Bacteria And Fungi ◽  
Total Fungi ◽  
Number Of Bacteria ◽  
Total Bacteria ◽  
Range Test

ABSTRAK. Penelitian efek penambahan bentonit dalam proses pelleting terhadap total bakteri dan total fungi dari produk pellet limbah penetasan sebagai bahan pakan alternatif, telah dilaksanakan di Laboratorium Teknologi Pakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro. Penambahan bentonit pada proses pelleting diharapkan mengurangi total bakteri dan total fungi pada pellet olahan limbah penetasan, sehingga aman digunakan sebagai bahan pakan alternatif. Pada penelitian ini limbah penetasan yang berupa cangkang telur, telur infertile, telur gagal menetas, serta DOC afkir dan mati dihancurkan, dicampur dengan bentonit dan dibuat pellet, untuk kemudian dianalisis kandungan total bakteri dan total fungi pada produk pellet. Perlakuan bentonit dilakukan dengan aras 0, 2, 4 dan 6% (B/B). Penelitian dilakukan dengan rancangan acak lengkap (RAL) 4 perlakuan dan 5 ulangan. Data dianalisis dengan analisis ragam dan dilanjutkan dengan uji wilayah ganda Duncan untuk mengetahui perbedaan antar perlakuan. Hasil menunjukkan bahwa pemberian bentonit dari berbagai level berpengaruh sangat nyata (P<0,01). Pemberian bentonit pada tingkat tertinggi (6%) mampu menekan total bakteri dan total fungi pada kisaran 105 cfu/g. Disimpulkan bahwa bentonit mampu menekan kandungan total bakteri dan total fungi pada produk pellet limbah penetasan. (Numbers of total bacteria and total fungi of hatchery waste pellets that made by adding bentonite) ABSTRACT. A Research about the effect of bentonite addition in the pelletizing process on total bacteria and total fungi of pellet product of hatchery waste as an alternative feedstuff, was done at Feed Technology Laboratory, Faculty of Animal and Agricultural Sciences, Diponegoro University. The addition of bentonite was expected to reduce the number of bacteria and fungi in the pellet product of hatchery waste, therefore, it could be used safely as an alternative feedstuff. In this experiment, the hatchery waste in the forms of eggshell, infertile eggs, un-hatched eggs, dead and culled DOC were blended, mixed with bentonite and pelleted,and then the number of total bacteria and total fungi of pellet was analyzed. The treatment was carried out with the level of bentonite i.e. 0, 2, 4 and 6% (w/w). Completely randomized design (CRD)was applied by 4 treatments and 5 replications of each. Data analyzed by analysis of variance (ANOVA), and followed by Duncan multiple range test (DMRT) to determine differences among the treatments. The results showed that administration of bentonite at various levels significantly affect the number of bacteria and fungi in the hatchery waste pellets (P <0.01). Administration of bentonite at the level of 6% shows the highest value on suppressing the content of total bacteria as well as fungi up to the range of total content 105cfu/g. In conclusion, the bentonite was able to suppress the total amount of bacteria and total fungi in the hatchery waste pellets.

scholarly journals Efikasi Vaksinasi pada Benih Ikan Nila (Oreochromis niloticus) dengan Metode Infiltrasi Hiperosmotik untuk Mencegah Infeksi Streptococcus agalactiae (THE EFFICACY OF VACCINATION ON TILAPIA SEEDS OF (Oreochromis niloticus) USING HYPEROSMOTIC INFILTRATION

2018 ◽  
Vol 18 (4) ◽  
pp. 634 ◽  
Author(s):  
Amalia Putri Firdausi ◽  
Sukenda Sukenda ◽  
Sri Nuryati
Keyword(s):  
Immune System ◽  
Antibody Level ◽  
Specific Antibody ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Salinity Treatment ◽  
Post Vaccination ◽  
Randomized Design ◽  
And Control

The objective of this study was to evaluate the effectiveness of vaccination on tilapia seedlings resulted from the vaccinated parent by hyperosmotic infiltration method at four different salinity to prevent Streptococcus agalactiae bacterial infection. A total of 100 seeds aged 20 days from the vaccinated mother were immersed in four different salinity: 0 ppt (control), 10 ppt, 20 ppt, and 30 ppt for five minutes, then removed and transferred into vaccine-containing containers for 30 minutes. Further maintenance was performed in freshwater aquariums and at days 10, 20, and 30 post vaccination, the immune system performance: Relative Percent Survival (RPS) after the challenge test, specific antibody level, and lysozyme was observed. The study was conducted using a complete randomized design with three replications. The results showed that the 10 ppt salinity treatment gave the best results compared to the others and control. The final RPS (10 ppt) value was 84.72%, 66.49%, and 47.06%, on the 10th, 20th, and 30thh days of vaccination, respectively. Specific antibody levels (10 ppt) were 0.077, 0.078, and 0.077 and lysozyme 0.092, 0.084, and 0.032 at days 10, 20, and 30 post vaccination, respectively. The value of RPS and specific antibody level at 10 ppt salinity was significantly different (P <0.05) compared to the other treatments, while the lysozyme treatment activity of 10 ppt was not significantly different compared to the 20 ppt treatment. Vaccination using 10 ppt saline hyperosmotic infiltration can improve the performance of the immune system by improving maternal immune protection against Streptococcus agalactiae infection.

scholarly journals Stocking densities and feeding strategies in shrimp and tilapia polyculture in tanks

2013 ◽  
Vol 48 (8) ◽  
pp. 1088-1095 ◽  
Author(s):  
Bruno Rodrigo Simão ◽  
Luis Otavio Brito ◽  
Alex Sandro Campos Maia ◽  
Laizy Cabral Miranda ◽  
Celicina Maria da Silveira Borges Azevedo
Keyword(s):  
Oreochromis Niloticus ◽  
Feeding Strategies ◽  
Feed Conversion ◽  
Shrimp Culture ◽  
Main Crop ◽  
Randomized Design ◽  
Marine Shrimp ◽  
Completely Randomized Design ◽  
Feed Conversion Rate ◽  
Fish Performance

The objective of this work was to evaluate the performance of Pacific marine shrimp (Litopenaeus vannamei) and tilapia (Oreochromis niloticus), in a polyculture in tanks subjected to different stocking densities and feeding strategies, in comparison with monoculture. Two experiments were performed, at the same time, in a completely randomized design with three treatments and four replicates each. Treatments for experiment I were: monoculture with 10 shrimp per m² (10S:0T); polyculture with 10 shrimp and 0.5 tilapia per m² (10S:0.5T); and polyculture with 10 shrimp and 1 tilapia per m² (10S:1T). Shrimp was the main crop, and feed was provided based on shrimp biomass. Treatments for experiment II were: monoculture with 2 tilapia per m² (2T:0S); polyculture with 2 tilapia and 2.5 shrimp per m² (2T:2.5S); and polyculture with 2 tilapia and 5 shrimp per m² (2T:5S). Tilapia was the main crop, and feed was provided based on fish requirements. In the experiment I, tilapia introduction to shrimp culture resulted in lower shrimp growth and poor feed conversion rate. In experiment II, shrimp introduction to tilapia culture did not interfere with fish performance. Polyculture is more efficient with the combination of 2 tilapia and 2.5 or 5 shrimp per m² and feed based on fish requirements.

scholarly journals THE EFFECT OF GIVING ONION (Allium Sativum) EXTRACT TO TREAT THE SEED OF TILAPIA (Oreochromis Niloticus) BACTERIAL INFECTED Aeromonas hydrophila

2021 ◽  
Vol 1 (2) ◽  
pp. 80-87
Author(s):  
Ani Lestari ◽  
Nurliah ◽  
Baiq Hilda Astriana
Keyword(s):  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Aquatic Environment ◽  
Allium Sativum ◽  
Bacterial Resistance ◽  
Survival Rates ◽  
Garlic Extract ◽  
Percent Survival ◽  
Randomized Design ◽  
Prevention And Treatment

MAS disease (Motile Aeromonas Septicemia) is a disease caused by A. hydrophila bacteria that attack tilapia and other freshwater fish species. The use of antibotics and chemicals in the continuous prevention and treatment of this disease can damage the aquatic environment and can cause bacterial resistance to antibotics, so antibotic use becomes ineffective. The study used a Complete RandomIzed Design (RAL) with an experimental method consisting of 5 treatments and 3 repeats. Treatment in this study is (P1) without soaking garlic extract and without infected bacteria Aeromonas hydrophila, (P2) without soaking garlic extract and infected bacteria Aeromonas hydrophila, (P3) soaking garlic extract with a concentration of 1% and disinfection of bacteria Aeromonas hydrophila, (P4) immersion of garlic extract with a concentration of 1.5% and infected bacteria Aeromonas hydrophila, (P5) soaked garlic extract with a concentration of 2% and infected with Aeromonas hydrophila bacteria. The results showed that the SR value of tilapia fish in each treatment had values ranging from 0.0% to 66.7%. Relative percent survival (RPS) tilapia seeds in each treatment have values ranging from 0.0% to 66.7%. The conclusion of this study is that giving garlic extract with different concentrations has a real different influence on survival rates and relative percent survival (RPS).

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