scholarly journals Masculinization of Tilapia (Oreochromis niloticus) Using Methyl Testosterone Natural Hormone

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Rosmaidar R ◽  
Winaruddin W ◽  
Mimi Herlina
Keyword(s):  
Oreochromis Niloticus ◽  
Fish Larvae ◽  
Hormone Treatment ◽  
Control Fish ◽  
Control Group ◽  
Group Iii ◽  
Randomized Design ◽  
Natural Hormone ◽  
Male Sex ◽  
Completely Randomized Design

This study aimed to determine the effect of methyl testosterone natural hormoneon the rate of masculinization of tilapia (Oreochromis niloticus). The study used 270 tilapia larvae with the age of 7 days as experimental animals. Tilapia larvae were divided into 3 treatments; eachtreatment consisted of 30 larvae fish. This study was an experimental study using completely randomized design with 3 treatments and 3replications. Treatment I as control group was fed with pellets ad libitum, treatment II was given feed containing methyl testosterone naturalhormone, and treatment group III was given hormone treatment by soaking the fish larvae with methyl testosterone natural hormone for 20hours. Larvae were raised in accordance with applicable procedures. Observation was conducted when the larvae fish were at 60 days old andthe sex of fish can be observed. Parameter examined was the percentage of the male sex. The average (± SD) percentage of male tilapia in groupPI, PII, and PIII were 36.39±3.97, 66.72±6.13, and 82.37±6.87%, respectively. The results showed that the percentage of male sex of tilapia fedwith natural hormone of methyl testosterone through the diet and dipping was significantly different (P0.05) from control fish. In conclusion,the administration of natural hormone of methyl testosterone increased the percentage of the male sex of tilapia).____________________________________________________________________________________________________________________Key words: tilapia larvae, natural hormone methyl testosterone, the male sex percentage

scholarly journals Pengaruh Suplementasi Probiotik A3-51 terhadap Derajat Imunitas Oreochromis niloticus Didasarkan pada Angka Kuman pada Ginjal Setelah Uji Tantang dengan Aeromonas hydrophila dan Aeromonas salmonicida achromogenes

2006 ◽  
Vol 8 (2) ◽  
pp. 144
Author(s):  
Agus Irianto ◽  
Hernayanti Hernayanti ◽  
Ning Iriyanti
Keyword(s):  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Aeromonas Salmonicida ◽  
Control Fish ◽  
System Response ◽  
Randomized Design ◽  
Immune System Response ◽  
Completely Randomized Design ◽  
Number Of Bacteria

Objectives of this research was to know the effect of probiotic A3-51 supplementation on food to the total number of bacteria in kidney and mortality of Oreochromis niloticus after challenge test with Aeromonas hydrophila and Aeromonas salmonicida achromogenes. This research was consisted of two experiment, the first was to know the highest immunity level of fish based on the number of macrophage and its phagocytic activity. Meanwhile, the second was to know the immunity level of fish challenged with A. hydrophila and A. salmonicida achromogenes by intra-peritoneal injection. The experiment used Completely Randomized Design in triplicates. The result from the first experiment showed that the highest non spesific immune system response, by means number of macrophage, was found 21 days treatment. The second experiment showed that the highest total number of bacteria in kidney and mortality level were 20.23 x 108 cells/g and 46.67%, respectively, both was found in control fish injected intra-peritoneally with A. salmonicida achromogenes.

scholarly journals Masculinization of Banggai Cardinalfish (Pterapogon kauderni) using 17α-Methyltestosterone-enriched Artemia sp.

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (2) ◽  
pp. 135
Author(s):  
Muhammad Safir ◽  
Desiana Trisnawati Tobigo ◽  
Seftina F Mangitung ◽  
Madinawati Madinawati ◽  
Zainab Zainab
Keyword(s):  
Survival Rate ◽  
Hormone Treatment ◽  
Male Fish ◽  
Daily Growth ◽  
Randomized Design ◽  
Spawning Cycle ◽  
Male Sex ◽  
Completely Randomized Design ◽  
Central Sulawesi ◽  
Eggs And Larvae

Banggai Cardinalfish (Pterapogon kauderni) is one of the endemic fish from the waters of the Banggai Islands, Central Sulawesi Province. One factor challenging the production of P. kauderni larvae is the male fish will nurture the eggs and larvae for 30 days, hindering next spawning cycle. One effort to overcome this problem an increase of male fish number should be performed, for example through sex reversal. This study aimed to determine the effectiveness of the hormone 17α-methyltestosterone (17α-MT) through Artemia sp. to increase male sex percentage P. kauderni. This study used a completely randomized design by testing four treatments namely feeding Artemia sp. which has been soaked with 2.0 mg of the 17α-MT hormone for two- hours given to P. kauderni larvae for 10 days (A); 20 days (B); 30 days (C), and without hormone treatment 17α-MT (control). Each treatment included control was performed in triplicates. The percentage of male sex was 93.33 % at 30 days treatment and was higher (P<0.05) compared to controls (53.3 %). Additionally, the results showed that daily growth rate, biomass gain and survival rate of all treatments were not significantly different (P>0.05) with those of control. In conclusion, Artemia sp. contained 17α-methyltestosterone enhanced male percentage of P. kaurderni. Keywords: growth, male percentage, Artemia sp., survival rate, Pterapogon kauderni

scholarly journals Effect of Temperature Increase on Hemoglobin Concentration and Hematocrit Value of Tilapia Fish (Oreochromis niloticus)

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Zuhrawati N. A.
Keyword(s):  
Oreochromis Niloticus ◽  
Temperature Increase ◽  
Hemoglobin Concentration ◽  
Effect Of Temperature ◽  
Control Group ◽  
Blood Samples ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Fish Hemoglobin ◽  
Banda Aceh

This research aims to determine the effect of temperature increase on hemoglobin consentration and hematocrit value of tilapia fish. This research was conducted at Faculty of Veterinary Medicine Syiah Kuala University Banda Aceh. Samples used were blood from tilapia fish thatderived from 12 tilapia fish with weight range of 40-50 g. The research design used was a completely randomized design (CRD) unidirectionalpattern with 3 treatments and 4 replications. P1 as a control group was the treatment with aquarium temperature of 29±1C, P2 was thetreatment with aquarium temperature of 32±1o C, and P3 was the treatment with aquarium temperature of 35±1o C. All aquarium size in thisstudy was 80 cm x 60 cm x 40 cm with the height of water 30 cm. The treatment was carried out for 15 days and on day 16 blood samples werecollected. Blood was taken through the caudalis vein. Data were analyzed by analysis of variance (ANOVA) one way, followed by Tukey test.Mean (± SD) of tilpia fish hemoglobin levels (g/dl) on P1, P2, and P3 were 4.50±0.49, 5.08±0.79, and 6.08±0.94, respectively. Mean (± SD) oftilapia fish hematocrit value (%) in P1, P2, and P3 consecutively were 26.50±8.10, 33.25±11.78, and 40.25±9.04. Statistical analysis showed thatan increase in temperature showed no effect (P 0.05) to the hemoglobin levels of tilapia fish and highly significant to the hematocrit value (P0.01). It can be concluded that the increase of water temperature on 32±1° C and 35±1° C in can increase the hemoglobin levels andhematocrit value of tilapia fish.____________________________________________________________________________________________________________________Key words: tilaphia fish, hemoglobin levels, hematocri value, temperature o

scholarly journals Sublethal toxicity test of mercury (Hg) in the flesh and tissue of Tilapia (Oreochromis niloticus)

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (2) ◽  
pp. 99
Author(s):  
Yusnaini Yusnaini ◽  
Emiyarti Emiyarti ◽  
Indriyani Nur ◽  
Oce Astuti ◽  
Rahmad Sofyan Patadjai
Keyword(s):  
Tissue Damage ◽  
Oreochromis Niloticus ◽  
Toxicity Test ◽  
Control Group ◽  
Sublethal Toxicity ◽  
Mononuclear Cell Infiltration ◽  
Tubular Necrosis ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hg Accumulation

This study tested the sublethal toxicity of mercury (Hg) in the flesh and tissue of tilapia (Oreochromis niloticus). This research used an experimental method completely randomized design (CRD) with 3 treatments and 3 replications. The treatments used different Hg concentrations: Treatment A = 0.08 ppm; B = 0.16 ppm; C = 0.24 ppm; and the control group = without the addition of Hg. The parameters observed were the accumulation of mercury (Hg) in the flesh and tissue damage (histopathology) of the gills, kidneys and liver of tilapia. The results showed that the highest Hg accumulation was at the highest Hg concentration (treatment C = 0.24 ppm). Tissue damage was mainly found in the kidneys, liver and gills of fish exposed to Hg with indications of bleeding, tubular necrosis, vacuolization of epithelial cells, and mononuclear cell infiltration. The results showed that the toxicity of mercury to the organs increased with the increase in the concentration of Hg in water. Keywords : flesh, mercury, Oreochromis niloticus, sublethal, tissue

scholarly journals The Effect of Dipping Time in Natural Methyl Testosterone Hormone on Male Forming Succesiveness Process of Tilapia (Oreochromis niloticus) Larvae

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Rosmaidar Rosmaidar ◽  
Dwinna Aliza ◽  
Jesica Ramadhanita
Keyword(s):  
Statistical Analysis ◽  
Oreochromis Niloticus ◽  
Control Group ◽  
Forming Process ◽  
Average Percentage ◽  
Treatment Groups ◽  
Natural Hormone ◽  
Male Sex ◽  
Banda Aceh ◽  
Dipping Time

The purpose of this research was to know the influence of dipping time in natural methyl testosterone hormone (MT) on successful male forming process of tilapia (Oreochromis niloticus) larvae. This research used 360 7 days old tilapia larvae, purchased from cultivation of brackish water, Ujung Batee, Banda Aceh. The samples were divided into 4 treatment groups with three repetitions, they were P0 (control group without treatment), PII (Tilapia larvae were dipped in natural MT for 10 hours), PII (Tilapia larvae were dipped in natural MT for 20 hours), and PIII (Tilapia larvae were dipped in natural MT for 30 hours). All tilapia larvae were maintained until the age of 60 days, before the sex of the fish examined. The average percentage of male tilapia in group P0, PI, PII, and PIII were 50.28, 87.77, 90.06, and 95.05%, respectively. Statistical analysis showed that there were significant differences (P0.05) among P0, PI, PII, and PIII. Based on result of this research, it can be concluded that the longest dipping time the highest the percentage of male tilapia.Key words: larvae, tilapia, natural hormone methyl testosterone, the male sex percentage

THE SOURCES OF AUXIN HORMONE TO GROWTH OF SHOOT PINEAPPLE STEM CUTTING (Ananascomosus.L. Merr.)

2016 ◽  
Vol 1 (1) ◽  
pp. 45-52
Author(s):  
Palupi Puspitorini
Keyword(s):  
Water Concentration ◽  
Urine Concentration ◽  
Experimental Results ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Natural Hormone ◽  
Cow Urine ◽  
Urine Concentrations ◽  
Completely Randomized Design ◽  
Better Than

The aim of this study was to select the best sources of auxin of which it can stimulate the growth of shoots Pineapple plant cuttings. This research is compiled in a completely randomized design (CRD) with 4 treatments and 6 replications. The Data were statistically Analyzed by the DMRT. Level of treatment given proves that no treatment 0%, cow urine concentration of 25%, young coconut water concentration of 25% and Rootone F 100 mg / cuttings. The results showed that cow urine concentrations of 25% and Rootone F 100 mg give the best results in stimulating the growth of shoots pineapple stem cuttings. Experimental results concluded that the effect of this natural hormone were better than the shoots without given hormone.           

Pengaruh ovaprim, aromatase inhibitor, dan hipofisa terhadap kualitas telur ikan Lele Dumbo (Clarias gariepinus)

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Erwin A. Aziz ◽  
Ockstan Kalesaran
Keyword(s):  
Survival Rate ◽  
Experimental Design ◽  
Aromatase Inhibitor ◽  
Fish Larvae ◽  
Clarias Gariepinus ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference ◽  
Hatching Eggs

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae.   Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae

EFFECTIVENESS Daphnia sp. WHICH IS PELET FEEDING TO THE GROWTH OF SEMAH’S LARVAE Tor douronensis (Valenciennes, 1842)

2020 ◽  
Vol 5 (2) ◽  
pp. 23-27
Author(s):  
Dina Tri Madya Ningsih ◽  
Siti Hudaidah ◽  
Mas Tri Djoko Sunarno
Keyword(s):  
Survival Rate ◽  
Fish Larvae ◽  
Weight Growth ◽  
Randomized Design ◽  
Test Parameters ◽  
Absolute Growth ◽  
Absolute Length ◽  
Effective Doses ◽  
Endemic Fish ◽  
Completely Randomized Design

The semah fish (Tor douronensis) is an Indonesian endemic fish that has a long growth, which takes 4 years to grow from larvae to broodstock. This study aims know of Daphnia sp. pelet fed with effective doses to increase the growth of semah’s fish larvae. The study was conducted from March 25 to April 13, 2020 at the Instalasi Riset Plasma Nutfah, Cijeruk, Bogor, West Java. The experimental design used completely randomized design (CRD) 4 treatments and 3 replications, namely treatment A (Daphnia sp.+pelet feed 0 gr/ L), treatment B (Daphnia sp.+ pelet feed 1 gr / L), treatment C (Daphnia sp. + pelet feed 2 gr / L), treatment D (Daphnia sp. + pelet feed 3 gr / L). The larvae of 9 days in age were randomly stocked into 12 aquariums measuring 60x30x35 cm with a density of 50 fish/aquarium and fed in the form of Daphnia sp. enriched pelet feed according to the treatment of 50 ind/larvae by giving 3 times a day which is maintained for 20 days. The test parameters measured are absolute growth in length and weight, survival rate, and water quality. Based on the results of statistical analysis (Anova), the results showed that the treatment D gave the highest influence on the growth of semah’s fish larvae (P <0.05) with an absolute weight growth value of 85,3 mg, absolute length growth of 10.6 mm, and survival rate 99.33%.

scholarly journals The Effect Of Rajalele Probiotic On Survival Rate Of Fish Juvenile Pangasius pangasius, Oreochromis niloticus, Cyprinus carpio

2020 ◽  
Vol 6 (1) ◽  
pp. 88-95
Author(s):  
Marlina Doloksaribu ◽  
Robert Martua Simanjuntak ◽  
Ied Hidayani Parinduri
Keyword(s):  
Social Sciences ◽  
Experimental Study ◽  
Data Analysis ◽  
Survival Rate ◽  
Cyprinus Carpio ◽  
Oreochromis Niloticus ◽  
Fish Farming ◽  
Randomized Design ◽  
The Social ◽  
Completely Randomized Design

The use of probiotics is certainly one of the solutions in overcoming the problem of increasing seed in fish farming. One of them is the use of probiotic king catfish, in this study tested on catfish, tilapia and goldfish. This research is an experimental study. The design used in this study was a Completely Randomized Design with 4 treatments 3 replications. Data analysis used Statistical Package for the Social Sciences version 15. The results of the analysis of the highest seed survival rate were obtained on Pangasius pangasius (95%), on Oreochromis niloticus (83,33 %), and Cyprinus carpio (80%). The treatment of Rajalele probiotics has a very significant effect (very significant) Fanalysis (23.01) > Ftable 0.01 (7.59) on the graduation of Pangasius pangasius, Oreochromis niloticus, and Cyprinus carpio. The conclusion of this study shows that the survival rate the highest in Pangasius pangasius

scholarly journals PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 169
Author(s):  
Sri Puji Astuti Wahyuningsih ◽  
Virid Gibson ◽  
Alfiah Hayati
Keyword(s):  
Molecular Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Control Group ◽  
Protein Profiles ◽  
Testosterone Levels ◽  
Randomized Design ◽  
Sds Page ◽  
Elisa Technique ◽  
Completely Randomized Design

<em>The purpose of this research was to determine the effect of polysaccharide krestin</em> (<em>PSK) </em><em>on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of  15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of  60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.</em>

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