scholarly journals Effect of Dietary Amylose/amylopectin Ratio on Diarrhea and Gut Microorganism of Weaned Pigs Challenged With Escherichia Coli Lipopolysaccharide

2020 ◽  
Author(s):  
can yang ◽  
min wang ◽  
xiaowu tang ◽  
huansheng yang ◽  
fengna li ◽  
...  
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Acid Concentration ◽  
Block Design ◽  
Alpha Diversity ◽  
Valeric Acid ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Weaning Piglets

Abstract Background: Dietary amylose/amylopectin ratio (DAR) plays important role in piglets’ immune system. It is controversial whether diarrhea could be relieved by changing DRA in weaning piglets.Methods: A total of 60 (Landrace × Yorkshire) castrated male pigs (initial body weight 6.51 ± 0.64 kg) were allotted to 5 groups (1 pig/cage and 12 replicates/group) in a randomized complete block design according to their BW. Piglets were fed ad libitum with diets different in DAR (0.00, 0.20, 0.40, 0.60, 0.80) for 28 days. Feed transition occurs at day 15. 100 μg/kg body weight lipopolysaccharides or same amount of saline were injected via abdomen on day 29, 12 h before slaughter. Results: Diarrhea rate and diarrhea degree were higher when DAR was 0.40 than in groups 0.20 and 0.80 during third weed (P<0.05). Iso-valeric acid of ileum decreased after LPS stress (P<0.05). Cecal iso-butyrate acid and iso-valeric acid concentrations increased after LPS stress (P<0.05). Iso- and valeric acid concentration of ileal chyme was higher in group 0.20 compared with other groups (P＜0.05). Cecal acetic acid and propionic acid concentration were higher in group 0.40 than in group 0.20 (P＜0.05). Alpha diversity of cecal microbial representative by goods coverage was higher in group 0.40 when compared with group 0.20 (P＜0.05). At the genus level, the abundance of Ruminococcaceae_ NK4A214_ group and Anaerotruncus in cecal chyme of Group 0.20 was significantly higher than that in Group 0.40 (P<0.05), with no significant difference compared with other groups (P>0.05). The abundance of Cetobacterium was significant lower in cecal chyme from Group 0.20 compared with pigs from Group 0.40(P<0.01), with no significant difference compared with other groups (P>0.05). Diarrhea rate during third week negative correlated with abundance of Rikenellaceae_RC9_ gut_ group and X.Eubacterium_coprostanoligenes_group (P<0.05). Conclusions: Diarrhea happened when weaned piglets received diet with DAR 0.40 during feed transition, but they will regulate cecal microorganism and metabolites such as acetic acid and propionic acid to restore their health.

Effect of Bio and Organic Fertilizers on Pumpkin's (Cucurbita pepo L.) Leave Content of N, P, and K

2020 ◽  
Vol 10 (1) ◽  
pp. 241-245
Author(s):  
Ammar Hameed Madi ◽  
Jawad A. Kamal Al-Shibani
Keyword(s):  
Cucurbita Pepo ◽  
Sandy Loam ◽  
Block Design ◽  
Organic Fertilizers ◽  
Flowering Stage ◽  
College Of Agriculture ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Loam Soil ◽  
Four Levels

This study was conducted to investigate the effect of bacterial bio-fertilization A. chroococcum and P. putide and four levels of compost (0, 1, 2, 3) tons.h-1 on the leaves content of N.P.K elements. The experiment was carried out in one of the greenhouses of the College of Agriculture - University of Al-Qadisiyah during fall season 2018-2019. It designed in accordance with the Randomized Complete Block Design (RCBD) with three replicates in sandy loam soil. The means of treatments were compared with the least significant difference (LSD) at (5)% probability level. The results present that the treatments of A. chroococcum, P. putide and compost at (3) tons.kg-1 significantly increases the leaves content of K.P.K compared to all other treatments in the flowering stage (4.970, 0.5000, and 4.930) mg.kg-1, respectively. This treatment was followed by the effect of the treatment of A. chroococcum and compost at (3) tons.kg-1, which increases the values of all traits except the leaf content of (P). Bio-fertilizer with P. putide + A. chroococcum significantly increases the leaves' content of P.

scholarly journals EFFECT OF UNILATERAL NEPHRECTOMY ON KIDNEY FUNCTION IN FERAL DOMESTIC CATS (Felis catus)

2019 ◽  
Vol 7 (2) ◽  
pp. 101-107
Author(s):  
Dionesio A. Estopa
Keyword(s):  
Kidney Function ◽  
Block Design ◽  
Age Groups ◽  
Unilateral Nephrectomy ◽  
Domestic Cats ◽  
Experimental Animals ◽  
Operative Care ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
One Year

This experimental study was conducted to determine the effect of unilateral nephrectomy on kidney function through hematological and urological values after surgery using feral domestic cats.  Four (4) apparently healthy stray male cats were caught and used as experimental animals in this study. The animals were grouped into two (2) with two cats in each group. Grouping of the animals was based according to age, the 1st group – ages one year and above (>1 year) and the 2nd group – ages one year and below (<1 year).  A split plot randomized complete block design was used in the experiment. The result shows that rectal temperature and packed cell volume have been affected on both age group of cats and other urological values like the presence of urobilinogen, protein, leukocytes, erythrocytes and crystals were elevated twenty four hours after surgery. A significant increase on the level of creatinine and blood urea nitrogen has been noted from the 3rd and 15th post – operative day. However, no significant difference was noted between experimental animals and the two age groups, all of which have similar result. The findings of the study revealed that the remaining kidney could not completely compensate the function of the other kidney up to the 15th post – operative day, it may take longer than fifteen days. Hence, proper supportive treatment, post-operative care & management of animals subjected to unilateral nephrectomy is a must and should continue beyond fifteen days.

scholarly journals EFFECT OF HARDWOOD BARK MULCH ON THE WINTER SURVIVAL OF EIGHT CULTIVARS OF GARDEN CHRYSANTHEMUMS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1097c-1097
Author(s):  
William F. Hayslett ◽  
P. R. Thangudu ◽  
Sabrina Shaw
Keyword(s):  
Field Study ◽  
Block Design ◽  
Winter Survival ◽  
Research Station ◽  
Percent Survival ◽  
Flowering Season ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Cold Hardy

A field study was conducted at Tennessee State University's research station to evaluate the effect of hardwood bark mulch on the winter survival of garden mums. A randomized complete block design was used. Cultivars used were adorn, encore, grandchild, jackpot, legend, minnautumn, minnwhite and triump. At the end of the flowering season the tops were removed leaving a four inch stubble in the mulch. The number of mum plants that resumed growth the following spring were counted for each cultivar. There was a difference in the winter survival of the different cultivars as well as a significant difference in the mulch treated and the control. Grandchild and jackpot were most cold hardy followed by encore, minnwhite, minnautumn, triump, legend, and adorn. Grandchild and jackpot with four inches of hardwood bark mulch had an 88 percent survival while the control had a 44 percent survival. Adorn. had a 51 percent survival with four inches of mulch and a 20 percent survival in the control. This data shows that hardwood bark mulch holds a great potential for providing excellent winter protection for garden mums.

scholarly journals PSIV-16 Effect of a 1-monoglyceride, 1,2-diglyceride of butyric-, caproic- and caprilic acid, and Avilamycin on performance and intestinal E. coli when fed to piglets challenged with E. coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 188-189
Author(s):  
Michael Coelho ◽  
Peter Ader
Keyword(s):  
Caprylic Acid ◽  
Block Design ◽  
Random Effect ◽  
Fixed Effect ◽  
Experimental Unit ◽  
Infection Model ◽  
E Coli ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
T1 And T2

Abstract This study examined the effect of 1-monoglycerides and 1,2-diglycerides of butyric-, caproic- and caprylic acid (SILOHealth™ 104L [monoglycerides]) and avilamycin on day 14 and 21 post weaning performance of E. coli (GN-Ent and O157:H7) day 5 challenged piglets fed corn/soy diets that met or exceeded current NRC (2012) recommendations. A total of 96, Choice Genetics CG32, piglets were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (4 piglets/pen x 3 treatments x 8 replications). Pigs were blocked by sex and weight and were orally dosed with 5 ml (1x109 CFU/g, strain GN-Ent and O157:H7) E. coli d 5 after weaning (Ec). The treatment were: NC with Ec; T1=NC + 3 kg/MT monoglycerides; T2=NC+ 80 ppm avilamycin. Least significant difference was used to compare means of treatment. Mortalities and pen removals were weighed and recorded. The E. coli infection model was effective in causing E. coli mortality of 25% at d14 post weaning and 33% at d21 post weaning in NC. T1 and T2 reduced mortality versus NC at d14 (25, 3 and 0%, P < 0.05) and d21 (33, 4 and 0%, P < 0.05), increased ADG at d14 (203, 255 and 259 g, P < 0.05) and d21 (456, 511 and 515 g, P < 0.05), decreased FCR at d21 (1.90, 1.85 and 1.83, P < 0.05), and decreased intestinal E. coli, log 10 of CFU/g, versus NC (4.1, 2.2 and 2.1, P < 0.05). Monoglycerides and avilamycin performance, mortality and intestinal E. coli log 10 were not significantly different at P < 0.05. In conclusion, the use of monoglycerides improved ADG, reduced FCR, reduced mortality and reduced intestinal E. coli log 10 at day 14 and 21 post weaning versus NC and monoglycerides were not significant different (P < 0.05) than avilamycin.

scholarly journals PSX-29 Levels of hay and narasin inclusion on carcass characteristics and meat composition

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 455-455
Author(s):  
Lairana A Sardinha ◽  
Daniel M Polizel ◽  
Alexandre A Miszura ◽  
Arnaldo C Limede ◽  
José P R Barroso ◽  
...  
Keyword(s):  
Body Weight ◽  
Block Design ◽  
Carcass Characteristics ◽  
Ash Content ◽  
Carcass Weight ◽  
Initial Body Weight ◽  
Composition Data ◽  
Meat Composition ◽  
Randomized Complete Block Design ◽  
Different Levels

Abstract The objective of this study was to determinate the effects of narasin on carcass characteristics of lambs fed a diet containing different levels. Forty-four lambs were allotted in a randomized complete block design, defined by initial body weight (BW), in a 2x2 factorial arrangement. The first factor was forage (coastcross hay) inclusion (10 or 20%, DM basis) and the second factor was narasin (0 or 13 ppm). The experimental diets were isonitrogenous (17.4%, DM basis). The experiment lasted 112 days. At the end of the experiment, the lambs were slaughtered to evaluate the carcass characteristics and meat composition. Data were analyzed using the MIXED procedure of SAS and the LSMEANS option was used to obtain the means. The effects were considered significant when P &lt; 0.05. There was no interaction between forage levels and narasin inclusion. The experimental diets did not affect the slaughter BW (54.8 ± 1.6 kg), hot carcass weight (29.3 ± 0.96 kg), dressing percentage (53.5 ± 0.67%) and LM area (18.8 ± 0.89 cm2). The inclusion of narasin increased 12th-rib fat (2.10 vs 2.75 mm; P &lt; 0.01) and BW thickness (16.9 vs 20.2 mm; P &lt; 0.01). The inclusion of 20% of hay decreased BW thickness (19.21 vs 17.9 mm; P = 0.03). The experimental diets did not affect the moisture (74.2 ± 0.53%) and ash content in meat (1.27 ± 0.04%). Narasin inclusion increased the fat (2.81 vs 3.62%; P &lt; 0.01) and decreased the CP (21.3 vs 20.7%; P = 0.02) in meat composition. The inclusion of 10% of hay increased the fat in meat (3.5 vs 2.9%; P = 0.03), however, did not affect the CP content. In conclusion, the narasin inclusion and levels of hay improve the carcass characteristics and change the meat composition.

Growth and Aflatoxin Production by Aspergillus parasiticus NRRL 2999 in the Presence of Acetic or Propionic Acid and at Different Initial pH Values

1987 ◽  
Vol 50 (11) ◽  
pp. 909-914 ◽  
Author(s):  
GULAM RUSUL ◽  
FATHY E. EL-GAZZAR ◽  
ELMER H. MARTH
Keyword(s):  
Acetic Acid ◽  
Propionic Acid ◽  
Aflatoxin B1 ◽  
Maximum Concentration ◽  
Aspergillus Parasiticus ◽  
Dry Weight ◽  
Aflatoxin Production ◽  
Lag Phase ◽  
Significant Difference ◽  
Initial Ph

Experiments were done to determine effects of different concentrations of acetic or propionic acid in a glucose-yeast extract-salts medium with an initial pH value of 4,5 or 5.5 on growth and aflatoxin production by Aspergillus parasiticus NRRL 2999. Amounts of aflatoxin were measured with reversed-phase high-performance liquid chromatography. The maximum concentration of acetic or propionic acid that permitted growth at an initial pH of 5.5 was 1% after 7 d of incubation and 0.25% after 3 d of incubation, respectively. When the initial pH of the medium was 4.5, the maximum concentration of acetic or propionic acid that permitted growth was 0.25 or 0.1%, respectively. There was no significant difference (p&gt;0.05) in amount of mycelial (dry weight) produced by cultures in the presence of 0.0, 0.25, 0.50 or 0.75% acetic acid. Amounts of aflatoxin B1 and G1 produced decreased with an increasing concentration of acetic acid. Increasing concentrations of propionic acid caused a decrease in the amount of mycelial dry weight and aflatoxin produced by cultures growing in the medium with an initial pH of 5.5. At an initial pH of 4.5 mycelial growth was slow and at 3 d of incubation amounts of aflatoxin B1 and G1 produced were reduced as concentrations of acetic acid increased. This also was true for propionic acid in the medium with an initial pH of 4.5. Cultures with an extended lag phase in the presence of acetic or propionic acid overcame this and then produced large amounts of aflatoxin B1 and G1 at 7 and 10 d of incubation.

scholarly journals Performance of Six Genotypes of Tritordeum Compare to Bread Wheat under East Mediterranean Condition

2020 ◽  
Vol 12 (22) ◽  
pp. 9700
Author(s):  
Ioanna Kakabouki ◽  
Dimitrios F. Beslemes ◽  
Evangelia L. Tigka ◽  
Antigolena Folina ◽  
Stella Karydogianni ◽  
...  
Keyword(s):  
Bread Wheat ◽  
Block Design ◽  
Wheat Yield ◽  
Significance Level ◽  
Yield And Quality ◽  
Gluten Content ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Dry Conditions ◽  
Set Up

Four advanced tritordeum lines were studied and compared to two commercial varieties of tritordeum and wheat cultivars in yield and quality features, in Greece. For this purpose, a two-year experiment was established in the Greek territory. The field experiment was set up in Randomized Complete Block Design (RCBD) with blocks, with different lines and varieties. Head emergence was calculated based on Growing Degree Days (GDDs), which was significantly affected by the year. The significance of differences between treatments was estimated by using Fisher’s least significant difference (LSD) test with significance level p = 0.05. The weight of 1000 seeds of the two commercial varieties differed from the equivalent weight of 1000 seeds of tritordeum lines, approximately 1 g. Yield was significantly affected by lines/varieties and year. Compared to tritordeum yield, wheat yield marked the highest values. This difference was almost 2 kg ha−1. Gluten content was significantly affected by lines/varieties. The highest gluten content was firstly noticed at wheat GENESIS (34.2%) variety and secondly at tritordeum Aucan (33.2%) variety. Protein was higher in tritordeum lines HT-1704 (15.5%), HT-1707 (15.1%) and Aucan variety (15.2%) during the first experimental year. Tritordeum seems to have significant adaptability to dry conditions in Greece and significant yields compared to the existing commercial varieties and bread wheat.

Dry matter yield and chemical content of forage sorghums intercropping with annual legumes.

1969 ◽  
Vol 96 (3-4) ◽  
pp. 165-181 ◽  
Author(s):  
Raphael W. Colbert ◽  
Elide Valencia ◽  
James S. Beaver
Keyword(s):  
Block Design ◽  
Mucuna Pruriens ◽  
Lablab Purpureus ◽  
Mean Values ◽  
Annual Legumes ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Fiber Fractions ◽  
Chemical Content ◽  
Sorghum Bicolor L

Forage sorghum [Sorghum bicolor L. Moench 'Brown midrib' (BMR)] and annual legumes lablab [Lablab purpureus L. 'Rongai' (L)] and mucuna [Mucuna pruriens L. 'Vine 90 d' (M)] are suitable fodder for the dairy industry in Puerto Rico, but BMR harvested at 90 days, usually has low crude protein (CP, 6%) content, which limits its usefulness. There is the possibility of increasing the CP by intercropping legumes with BMR, but this option has been only slightly investigated. This study proposes to compare BMR in monoculture and in intercropping with 'Rongai' (SL) and 'Vine 90 d' (SM), in addition to L and M in monoculture, in terms of total leaf mass (FM) and botanical components (sorghum, legumes and weeds) and chemical (CP and fiber fractions) at two harvest times. The experimental plots (25 m2) were sown in February and August 2008 in a randomized complete block design with five replications. At each harvest time, 2 m2 MF area was sampled 90 days after sowing. Data were analyzed by using the MIXED procedure of SAS and mean separation tests were performed by using F-protected LSD at 95% level of confidence. No significant differences (combining fodder botanical component and weeds) were detected for the total MF between BMR and BMR associated with legumes in monoculture, with mean values of 8.94, 8.81, and 8.42 Mg/ha for BMR-'Rongai', BMR-'Vine 90 d', and BMR, respectively. Overall, there was no significant difference in MF between May and August (8.1 vs. 7.5 Mg/ha). 'Rongai' yield (Mg/ha) did not change between May and August (3.6 vs. 3.0) but was lower in the intercrop (2.74) than in monoculture (3.94). 'Vine 90 d' had the lowest yield of 2.8 and 1.3 Mg/ ha in May and August, respectively. No differences (P > 0.05) in NDF and ADF content between BMR and BMR-'Rongai'-'Vine 90 d', whose values were 60.1 and 63.9% and 40.1 and 46.5%, respectively. However, differences (P < 0.05) were found in CP content between monoculture and intercropping, with values of 14.1, 11.1, and 6.0% for 'Rongai', 'Vine 90 d', and sole BMR, respectively, whereas the BMR-'Rongai' and BMR- 'Vine 90 d' CP were 9.8 and 9.1%, but these last values exceed by three units the percentage in BMR. In conclusion, BMR intercropped with 'Rongai' and 'Vine 90 d' improved the forage chemistry and helps to control weeds while favoring the performance of desirable botanical components.

scholarly journals Nutrient Yield of Brown Hemp and Its Utilization as Protein Source in Concentrate on Brahman×Thai-Native Cattle Performances

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Supreena Srisaikham ◽  
◽  
Pipat Lounglawan ◽  
Keyword(s):  
Animal Feed ◽  
Block Design ◽  
Average Daily Gain ◽  
Nutrient Composition ◽  
Feed Resources ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Nutrient Yield ◽  
Native Cattle ◽  
Daily Gain

Two experiments were conducted to evaluate the effects of cutting ages and height on the nutrient composition and yield of brown hemp (BH) in concentrate diets on Brahman×Thai-Native cattle performances. The first experiment was to determine the effects of cutting ages (30, 40 and 50 days) and height (30, 40 and 50 cm) on the nutrient composition and yield of BH. The experiment was a 3×3 factorial arrangement in a randomized complete block design. This study clearly showed that cutting ages at 50 days had a greater effect on the nutrient composition and yield than at 30 and 40 days, but the cutting heights did not reveal any significant differences in yield. However, the BH cutting for animal feed before plowing, besides improving the soil for green manure, also benefited feed resources. The second experiment was to study the effects of BH meal (BHM) in concentrate on Brahman×Thai-Native cattle performances. Twelve cattle, averaging 252 ± 18 kg body weight and at ages of 16-18 months were stratified randomly and assigned to one of the three feeding treatments. Cattle were fed BHM in different ratios: the treatments were 0, 10, and 20% of BHM, respectively. There were no significant differences in the dry matter intake among the treatments of 0, 10 and 20% of BHM in concentrate rations. For average daily gain, the minimum response observed was a significant difference at the highest level of 20% of BHM. Therefore, 10% of BHM can be used in the concentrate rations for beef cattle.

scholarly journals Response of Rapes (Brassica napus L.) to Nano- Iron Fertilization under semi-arid Region conditions

Bionatura ◽  
2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Lamiaa A. Mutlag ◽  
Nagam A. Meshaimsh ◽  
Hasan H. Mahdi ◽  
Raghad S. Mouhamad ◽  
Hasan H. Khamat ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Agricultural Research ◽  
Block Design ◽  
Nutrient Content ◽  
Brassica Napus L ◽  
Arid Conditions ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Semi Arid Region ◽  
Semi Arid

The field experiment was conducted in the season of 2017-2018 at the Agricultural Research Department-AL-Azafrinih/Baghdad-Iraq. This study aimed to determine the effect of adding the Foliar Fe fertilization (Nano-Fe) and ground fertilization of (N, P) in the growth and production yield of rapes (Brassica napus L.). The experiment included three levels of foliar fertilization (Nano-Fe) that is (0, 5, 10 Kg. ha-1) and one level of ground fertilization (N 100 Kg. ha-1, P 250 Kg. ha-1). The experiment designed according to Randomized Complete Block Design (R.C.B.D) with three replications each parameter. The studied traits were nutrients content (macro-micro) in seeds and soil, the percentage of protein, oil, and carbohydrate. All data were analyzed and used statistically. Results showed a significant difference between Nano-Fe fertilization and nutrient content (N, Mn, K, Ca, Mg, S, and Cu), pH in soil at the level of 10 kg. ha-1, compared to no-fertilization. Moreover, the significant difference between Nano-Fe fertilization, yield (1425.6 kg. ha-1), the percentage of oil (52.16%), protein content in seeds (46.5%), carbohydrate (26.8%), Nutrient content N and P in seeds at the level of 10 kg. ha-1, compared to no-fertilization and the nutrient content (Ca, Mg, Fe) in seeds at levelof5 kg. ha-1, compared to no-fertilization. Current results suggested that Nano-Fe fertilization at the level of 10 kg. ha-1 can be adopted as the best level for rapes (Brassica napus L.) cultivation under semi-arid conditions.

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