scholarly journals Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows

2020 ◽  
Author(s):  
Kun Hou ◽  
Jinjin Tong ◽  
Hua Zhang ◽  
Shan Gao ◽  
Yuqin Guo ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Dairy Cows ◽  
Dairy Cow ◽  
Milk Fat ◽  
Enrichment Analysis ◽  
Control Group ◽  
Milk Samples ◽  
Metabolite Profiles ◽  
Metabolomics Analysis ◽  
Potential Biomarkers

Abstract This study aimed to explore the effects of artemisinin (ART) on the milk microbiome and metabolites of dairy cow. A total of 12 mid-lactation Holstein dairy cows with similar parity, days in milk were randomly divided into 2 groups receiving either a total mixed ration (TMR) as the control group or this TMR and 120 g/d/head ART as the ART group. The milk samples were collected weekly to determine the contents, and end-of-trial (wk 8) milk samples were used to identify microbial species and metabolite profiles by 16S rRNA and LC-MS analyses, respectively. We observed that the milk fat content significantly increased by ART treantment (P < 0.05). The bacterial community richness was significantly lower in the ART group (P < 0.05), while the diversity showed no difference (P > 0.05). Compared with its abundance in the CON group, Firmicutes was significantly decreased, whereas Proteobacteria was significantly increased. Furthermore, in the ART group, the relative abundances of the genera Aerococcus, Staphylococcus, Corynebacterium_1 and Facklamia were significantly lower (P < 0.01). Metabolomics analysis revealed that ART significantly increasing the concentrations of glycerophospholipids, glycerolipids and flavonoids compared with those in the CON group. An enrichment analysis of the different metabolites showed that ART mainly affected glycerophospholipid metabolism and the pantothenate and CoA biosynthesis pathways. These findings revealed that ART supplementation could affect the milk microbiota and metabolites, that glycerophospholipids and glycerolipids could be potential biomarkers in the milk response to ART feed in dairy cows, and that ART changes substances in milk by maintaining lipid metabolism in the mammary gland.

scholarly journals Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows

2020 ◽  
Author(s):  
Kun Hou ◽  
Jinjin Tong ◽  
Hua Zhang ◽  
Shan Gao ◽  
Yuqin Guo ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Dairy Cow ◽  
Milk Fat ◽  
Enrichment Analysis ◽  
Control Group ◽  
Milk Samples ◽  
Metabolite Profiles ◽  
Rrna Sequencing ◽  
Metabolomics Analysis ◽  
Potential Biomarkers

Abstract This study aimed to explore the effects of artemisinin (ART) on the milk microbiome and metabolites of dairy cow. A total of 12 mid-lactation Holstein dairy cows with similar parity, days in milk were randomly divided into 2 groups receiving either a total mixed ration (TMR) as the control group or this TMR and 120 g/d/head ART as the ART group. The milk samples were collected weekly to determine the contents, and end-of-trial (week 8) milk samples were used to identify microbial species and metabolite profiles by 16S rRNA sequencing and LC-MS analyses, respectively. We observed that the milk fat content significantly increased by ART treantment ( P <0.05). The bacterial community richness was significantly lower in the ART group ( P <0.05), while the diversity showed no difference ( P >0.05). Compared with its abundance in the control (CON) group, Firmicutes was significantly decreased, whereas Proteobacteria was significantly increased. Furthermore, in the ART group, the relative abundances of the genera Aer ococcus, Staphylococcus, Corynebacterium_1 and Facklamia were significantly lower ( P <0.01). Metabolomics analysis revealed that ART significantly increasing the concentrations of glycerophospholipids, glycerolipids and flavonoids compared with those in the CON group. An enrichment analysis of the different metabolites showed that ART mainly affected glycerophospholipid metabolism and the pantothenate and CoA biosynthesis pathways. These findings revealed that ART supplementation could affect the milk microbiota and metabolites, that glycerophospholipids and glycerolipids could be potential biomarkers in the milk response to ART feed in dairy cows, and that ART changes substances in milk by maintaining lipid metabolism in the mammary gland.

Relationships among macro-minerals, other selected serum markers of bone profile and milk components of dairy cows during late-lactation

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ryszard Mordak ◽  
Zbigniew Dobrzański ◽  
Robert Kupczyński
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Dry Matter ◽  
Inorganic Phosphorus ◽  
Serum Markers ◽  
Laboratory Diagnostics ◽  
Lactation Period ◽  
Milk Samples ◽  
Milk Components ◽  
Macro Mineral

AbstractTesting blood and milk parameters as well as analysing the relationships among these markers is very useful for monitoring the internal homeostasis and health in high-yielding dairy cows during various production periods. The aim of the study was to assess the correlations (relationships) among macro-minerals, such as calcium (Ca), inorganic phosphorus (P), magnesium (Mg), other selected bone profile markers, such as total protein (TP), albumin, activity of alkaline phosphatase (ALP) measured in serum and selected milk components such as number of somatic cells (SCC), colony-forming units (CFU), milk fat (MF), milk protein (MP), milk lactose (ML), dry matter (DM), non-fat dry matter (FDM) and milk production in late-lactation cows. Both blood and milk samples were collected from 11 clinically healthy milking cows during the late-lactation period. The cows were examined once a day for 3 consecutive days resulting in 33 sets of blood and milk samples for laboratory and statistical analysis. Significant correlations were observed between: Mg and MP, Mg and FDM, ALP and SCC, TP and SCC, TP and MP, TP and FDM, albumin and MP, albumin and FDM, P and Mg, Mg and albumin, and between TP and albumin. When monitoring macro-mineral homeostasis and mammary gland health, especially in intensively fed high-yielding dairy cows correlations between these markers should be considered. The revealed correlations can allow for deeper comparative laboratory diagnostics of homeostasis and can be especially useful for laboratory monitoring of the potential risk of subclinical macro-mineral deficiency in high-yielding dairy cows.

Weekly excretion of the mammalian lignan enterolactone in milk of dairy cows fed flaxseed meal

2009 ◽  
Vol 76 (4) ◽  
pp. 455-458 ◽  
Author(s):  
Nathalie Gagnon ◽  
Cristiano Côrtes ◽  
Hélène V Petit
Keyword(s):  
Mammary Gland ◽  
Dairy Cows ◽  
Baseline Level ◽  
Significant Interaction ◽  
Control Diet ◽  
Secoisolariciresinol Diglucoside ◽  
Milk Samples ◽  
Flaxseed Meal ◽  
Ruminal Microbiota ◽  
Feeding Regimen

Flaxseed meal (FM) is rich in the plant lignan secoisolariciresinol diglucoside (SDG) which is converted to the mammalian lignans enterodiol and enterolactone (EL) by ruminal microbiota. Feeding FM to dairy cows increases linearly EL concentration in milk but enterodiol is not detected. The objectives of the study were to determine the length of time to obtain peak EL concentration in the milk of dairy cows fed 20% FM and the length of time to return to EL baseline level in milk when cows are switched from high to low intake of flax SDG. A total of 12 multiparous lactating Holstein cows were assigned randomly to one of two feeding regimens: the control (CO) diet was fed for 6 weeks or the FM diet was fed from week 0 to 3 inclusive and then cows were switched to the control diet from week 3 to 6 inclusive. Milk samples were taken weekly for EL analysis. There was a significant interaction between feeding regimen and week for milk concentration of EL as a result of higher concentration of EL from week 1 to 3 for cows on the FM regimen compared with those on the CO regimen. Concentrations of milk EL on the FM regimen maintained uniform high levels from week 1 to 3 and they decreased significantly from week 3 to 4 when the CO diet was reintroduced in week 3. This study suggests that the conversion of SDG to the mammalian lignan EL and the transfer of EL to the mammary gland are well established after one week of feeding 20% FM in the diet of dairy cows and that milk concentration of EL returns to baseline level after one week of FM deprivation.

scholarly journals Effects of intramammary infection on whey proteinograms of sheep during lactation

2015 ◽  
Vol 35 (3) ◽  
pp. 230-236 ◽  
Author(s):  
Vânia F. Lemos ◽  
Eduardo L.S. Guaraná ◽  
José A.B. Afonso ◽  
José J. Fagliari ◽  
Paulo C. Silva ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Biochemical Characterization ◽  
Production Systems ◽  
Polyacrylamide Gel Electrophoresis ◽  
Whey Proteins ◽  
Sodium Dodecyl ◽  
Acid Glycoprotein ◽  
Milk Samples ◽  
Lactation Stage ◽  
Potential Biomarkers

The study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), α-lactalbumin, β-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and α-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and α-lactoalbumin. The most significant changes were observed in α-1-acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, α-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep.

scholarly journals Daytime Grazing in Mountainous Areas Increases Unsaturated Fatty Acids and Decreases Cortisol in the Milk of Holstein Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3122
Author(s):  
Jalil Ghassemi Nejad ◽  
Bae-Hun Lee ◽  
Ji-Yung Kim ◽  
Kyung-Il Sung ◽  
Hong-Gu Lee
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Milk Fat ◽  
Unsaturated Fatty Acids ◽  
Control Group ◽  
Milk Fatty Acid ◽  
Omega 3 ◽  
Mountainous Areas ◽  
Lactating Cows

The effects of grazing lactating cows in mountainous areas for 12 and 24 h compared with the confined indoor system were evaluated by examining the overall milk fatty acid and cortisol. Twenty-one dairy cows were allocated to three treatment groups: (1) control (confined management system in a free-stall barn; TMR based), (2) grazing for 12 h (12hG; TMR plus grazing pasture), and (3) grazing for 24 h (24hG; pasture-based feeding system). Dry matter intake was higher in the control and 12hG groups than in the 24hG group. The yields of total milk and the 3.5% fat-corrected milk were the lowest in the 24hG group. Milk fat was the highest in the 24hG group and higher in 12hG compared with the control group. Milk protein and lactose levels were the highest in the 12hG group. The highest somatic cell count was observed in the 24hG group. The saturated fatty acid levels were higher in the control group compared with the 12hG and 24hG groups. There was no difference in overall mono-unsaturated fatty acids between 12hG and 24hG groups. Poly-unsaturated fatty acids were higher in the 12hG group compared with the control and 24hG groups. There was no difference in omega-6 (ω-6) fatty acids among the groups, and omega-3 fatty acids were higher in the 12hG group than in the control group. Milk cortisol was the highest in the 24hG group and higher in the control group compared with the 12hG group. Taken together, grazing for 12 h is advisable for farms that have access to mountainous areas to improve the milk fatty acid profile and decrease the stress levels in high-yielding Holstein lactating cows.

scholarly journals Determination of organic components of milk as method for evaluating energy status of dairy cows

2010 ◽  
Vol 64 (1-2) ◽  
pp. 21-32
Author(s):  
Djordje Savic ◽  
D. Matarugic ◽  
N. Delic ◽  
D. Kasagic ◽  
M. Stojanovic
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Protein Concentration ◽  
High Yield ◽  
Energy Status ◽  
Milk Samples ◽  
Organic Components ◽  
Physiological Values ◽  
The Individual

The objective of the investigations described in this work was to determine the energy status and to make recommendations for correcting the cow diet at a farm of high-yield dairy cows, on the grounds of values for the concentration of organic components of milk and their ratios in individual milk samples. A total of 147 cows were examined, including 97 in the first and 50 in the second lactation. Average concentrations of milk fat and urea were within the physiological values. Namely, the milk fat concentration in cows in the first lactation was 38.88?5.07 g/l, and it was 36.47?4.82 g/l in cows in the second lactation, while the urea concentration in cows in the first lactation 3.16?0.58 mmol/l and it was 3.72?0.64 mmol/l in cows in the second lactation. The protein concentration in both groups of cows was below the physiological values, being 30.33?2.35 g/l in cows in the first lactation and 30.17?2.27 g/l in cows in the second lactation. Based on the ratio of urea and protein concentrations, as well as of fat and proteins in the individual milk samples, it was concluded that in most examined cows, both in those in the first and those in the second lactation, there is a deficit of energy, along with a deficit or relative surplus of proteins. On the grounds of the obtained results, recommendations were given for correcting the feed rations in the coming period. .

The efficacy of feeding a live probiotic yeast, Yea-Sacc®, on the performance of lactating dairy cows

2015 ◽  
Vol 3 ◽  
Author(s):  
D. Tristant ◽  
C. A. Moran
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Milk Protein ◽  
Feed Additive ◽  
Control Group ◽  
Target Dose ◽  
Lactating Dairy Cows ◽  
Farm Model ◽  
The Impact

SummaryThe following trial was conducted to evaluate the impact of feeding Yea-Sacc® (YS; Alltech Inc, USA), a zootechnical feed additive based on a live probiotic strain of Saccharomyces cerevisiae, to lactating dairy cows over a 12 week period. Sixty-four primiparous and multiparous Holstein dairy cows, grouped to give similar range of parity, physiological and milk production stages, were selected for the study. Cows were equally allocated to either a control feed group or a diet supplemented with YS (32 cows per treatment). The test diet was formulated to include YS (Yea-Sacc® Farm Pak) incorporated in the total mixed ration (TMR), supplying a target dose of 5 × 107 CFU/kg feed dry matter (DM). This target dose delivered 1 × 109 CFU/cow/day, for a cow consuming 20 kg feed (DM basis) daily. Each cow was considered a replicate unit. Cows were fed a nutritionally adequate total TMR plus hay and a supplementary protein/energy concentrate (calculated according to milk yield) for 12 weeks, supplied once a day after the morning milking. Weigh backs of feed were recorded daily, with refusals being maintained at 3% of the total intake. During the 12 week study period, YS had significant beneficial effects on milk production (+0.8 kg/day; P = 0.003), energy corrected milk production (+1.4 kg/day; P < 0.0001), synthesis of milk protein (+36 g/day; P = 0.001), milk protein content (+0.3 g/kg; P = 0.009), and milk urea content (−0.09 mg/l; P = 0.004). The synthesis of milk fat was similar between treatments but milk fat content was lower for the YS group compared to the control group (−1.1 g/kg; P = 0.0002). Lactose content was always higher (+0.8 g/kg; P < 0.0001) for the YS group, indicating enhanced energy utilisation. In general, the effect of YS was higher during the first study period (one to seven weeks), when cows were in early lactation and the production potential was higher. YS cows produced significantly more milk during the study, and an additional 220 kg milk per cow was sold from this group from the output measured from the beginning of the study to two weeks post-trial. However, the statistical analysis including the post-study period did not show a significant effect. The 305-day simulated milk production was higher for the YS group (+400 kg/cow) but again the difference was not significant. In conclusion, YS at a target dose of 5 × 107 CFU/kg DM improved milk production and milk quality in healthy dairy cows. In addition, when the data were included in a whole-farm model, feeding YS reduced methane emissions by 4%, reduced the number of animals required for the desired milk production by 4% and increased overall farm margins by 1.4%.

scholarly journals Effects of Saccharomyces cerevisiae Addition in Dairy Cows Diets

2021 ◽  
Vol 78 (1) ◽  
pp. 18
Author(s):  
Marinela ENCULESCU
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Health Status ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Control Group ◽  
Free Access ◽  
Alternative Source ◽  
Effective Measure ◽  
Experimental Group

The aim of this study was to evaluate the effects of fresh yeast (Saccharomyces cerevisiae) supplementation in the dairy cows’ diets on productive performances and health status. The study was carried out in the experimental farm of the Research and Development Institute for Bovine Balotești on 50 multiparous Romanian Black and Spotted dairy cows, randomly divided into two groups (N = 25 heads/group), according to age, milk yield, body weight and health status. The experimental group received 80 g Saccharomyces cerevisiae/head/day for one year. The groups were fed with the same diet and had free access to water and salt. Results were expressed as a mean (±Standard Deviation). The t-test was applied to obtain the significance of difference. Supplementation of the diet with Saccharomyces cerevisiae had a significant effect (P <0.001) on milk yield (20.71±1.65 l/head/day) for the experimental group comparing with the control group (18.22±1.81 l/head/day), and on milk protein and lactose (P <0.05). The addition of Saccharomyces cerevisiae in dairy cows’ diet did not improve the milk fat, hematological and biochemical/urine indicators in the experimental group (P >0.05). However, for alkaline phosphatase, differences at the end of the study have been observed (P <0.01). The beneficial effect of the yeast and yeast products in ruminants could be attributed to microbial activity by increasing the number of beneficial bacteria in the rumen of the animals. The use of Saccharomyces cerevisiae as an alternative source of economic protein, vitamins and minerals in dairy cows’ diet represents an effective measure to optimize animal productivity.

The influence of long-term dietary inclusion of the antibiotic Actaplanin on dairy cow performance

1988 ◽  
Vol 1988 ◽  
pp. 110-110
Author(s):  
M.M. Abubakar ◽  
P. Rowlinson
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Dairy Cow ◽  
Milk Fat ◽  
Rumen Fluid ◽  
Increase Milk Production ◽  
Cow Performance ◽  
A Performance

The antibiotic Actaplanin is a complex of glycopeptide compounds produced by Actinoplanes missourrensis. When included in feed as a performance enhancer it has been shown to increase milk production of dairy cows (McGuffey et al. 1983). Actaplanin is associated with reduced proportions of acetate and increased proportions of propionate in rumen fluid and a depression in milk fat proportion may result (Clapperton et al. 1987). The aim of the trial reported here was to monitor the effects of feeding 960 mg Actaplanin/head/d to dairy cows throughout two successive lactations.

scholarly journals Evaluating the Suitability of Hazelnut Skin as a Feed Ingredient in the Diet of Dairy Cows

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1653
Author(s):  
Manuela Renna ◽  
Carola Lussiana ◽  
Vanda Malfatto ◽  
Mathieu Gerbelle ◽  
Germano Turille ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
De Novo ◽  
Saturated Fatty Acids ◽  
Economic Value ◽  
Control Group ◽  
Feed Ingredient ◽  
Branched Chain ◽  
Quality Of Milk ◽  
Biohydrogenation Intermediates

Hazelnut skin (HS) was evaluated as a source of nutrients for dairy cows. In total, 26 Aosta Red Pied cows were divided into two balanced groups. All cows were fed hay ad libitum. The control group was also given 6 kg of concentrate, while the hazelnut skin group (HAZ) was given 1 kg of the same concentrate that was substituted by 1 kg of HS. The dry matter intake of the cows was reduced by the dietary inclusion of HS (p ≤ 0.001). The milk yield and main constituents were unaffected by treatment. Milk from HAZ cows showed decreased concentrations of de novo saturated fatty acids (FAs), odd- and branched-chain FA, α-linolenic acid, and long-chain n-3 FAs, as well as increased concentrations of stearic acid, oleic acid, linoleic acid, total monounsaturated FAs, trans biohydrogenation intermediates, and α–tocopherol. Replacing the concentrate with HS increased the human-inedible feed quota in the diet and improved the sustainability of milk production in terms of the food-feed competition. Our results suggest that it is possible to add economic value to organic waste from the hazelnut industry using HS as a feed ingredient for dairy cows, enhancing the feed efficiency and milk antioxidant activity and having expected impacts on the nutraceutical quality of milk fat.

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