Autotrophic denitrification with anaerobic Fe2+ oxidation by a novel Pseudomonas sp. W1

2015 ◽  
Vol 71 (7) ◽  
pp. 1081-1087 ◽  
Author(s):  
Huining Zhang ◽  
Hongyu Wang ◽  
Kai Yang ◽  
Qing Chang ◽  
Yuchong Sun ◽  
...  

In the present study, a novel Pseudomonas sp. W1 was characterized in terms of its ability to perform nitrate removal coupled with anaerobic Fe2+ oxidation under autotrophic growth condition. The effects of operating parameters with respect to the initial solution pH, temperature and initial Fe2+ concentration on nitrate removal were investigated by central composite design. Based on the results of response surface methodology, the maximal nitrate removal efficiency was achieved under the following conditions: pH 7.0, temperature 30 °C and initial Fe2+ concentration 1,100 mg L−1. Under this optimal condition and with an initial NO3−-N concentration of 55 mg L−1, this strain could remove NO3−-N with 90% reduction of NO3−-N, corresponding to oxidizing Fe2+ with 71% oxidation of Fe2+ after 7 days of incubation. The result of kinetic evaluation indicated that this bacterium showed significant substrate affinity to both NO3−-N and Fe2+.

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1370
Author(s):  
Zhenfeng Lin ◽  
Ziwei Pan ◽  
Yuhao Zhao ◽  
Lin Qian ◽  
Jingtao Shen ◽  
...  

PPy-Fe3O4/Kaolin was prepared with polypyrrole functionalized magnetic Kaolin by a simple, green, and low cost method to improve the agglomeration and low adsorption capacity of Kaolin. PPy-Fe3O4/Kaolin was employed to remove Hg2+ and the results were characterized by various methods. Relevant factors, including solution pH, dosage of adsorbent, concentration (C0), and temperature (T), were optimized by Response Surface Methodology (RSM) and Central Composite Designs (CCD). The optimal results show that the importance for adsorption factors is pH > T > C0 > dosage, and the optimal adsorption conditions of PPy-Fe3O4/Kaolin are pH = 7.2, T = 315 K, C0 = 50 mg/L, dosage of 0.05 g/L, and the capacity is 317.1 mg/g. The adsorption process conforms to the pseudo-second-order and Langmuir models. Dubinin–Radushkevich model shows that adsorption process is spontaneous and endothermic. Moreover, the adsorption of mercury by PPy-Fe3O4/Kaolin was achieved mainly through electrostatic attraction, pore diffusion, and chelation between amino functional groups and Hg2+. PPy-Fe3O4/Kaolin has excellent reproducibility, dispersity, and chemical stability, and it is easy to be separated from solution through an external magnetic field. The experiments show that PPy-Fe3O4/Kaolin is an efficient and economical adsorbent towards mercury.


2017 ◽  
Vol 18 (5) ◽  
pp. 1625-1634
Author(s):  
Jun feng Su ◽  
Ting ting Lian ◽  
Ting lin Huang ◽  
Dong hui Liang ◽  
Wen dong Wang

Abstract In this study, strain CC76, identified as Enterobacter sp., was tested for the reduction of Fe3+ and denitrification using immobilized pellets with strain CC76 as experimental group (IP) and immobilized pellets with strain CC76 and magnetite powder as experimental group (IPM) in the autotrophic denitrification immobilized systems (ADIS). Compared with IP, a higher nitrate removal rate was obtained with IPM by using three levels of influent Fe3+ (0, 5, and 10 mg/L), four levels of pH (5.0, 6.0, 7.0, and 8.0), and three levels of hydraulic retention time (HRT) (12, 14, and 16 h), respectively. Furthermore, response surface methodology (RSM) analysis demonstrated that the optimum removal ratios of nitrate of 87.21% (IP) and 96.27% (IPM) were observed under the following conditions: HRT of 12 h, pH of 7.0 and influent Fe3+ concentration of 5 mg/L (IP) and 1 mg/L (IPM).


Author(s):  
Meschack Mukunga Muanda ◽  
Pele Pascal Daniel Omalanga

A sulfate solution containing 1773.965 mg/L Mn2+, 3216.178 mg/L Mg2+ and 566.254 mg/L Ca2+ was used to perform the maximum recovery of manganese and minimum recovery of magnesium. Carbonate precipitation was used due to the better selectivity for manganese over magnesium and other impurities recovery compared to hydroxide precipitation. Four factors were studied: solution pH value, contact time, reaction temperature and sodium carbonate consumption. Analysis of variance (ANOVA) and response surface methodology (RSM) were used to determine the optimum. Under the optimum conditions, the manganese and magnesium recoveries were the highest and the lowest respectively, while the pH, the time, the temperature and the volume of Na2CO3 were the lowest.  The values of the four factors were found as followed: 8.9293, 60.69 min, 77.95°F, and 50.7650 mL respectively. Moreover, the recoveries of manganese and magnesium were 99.9799% and 4.3045% respectively. The results show that optimization using RSM is effective in improving carbonate precipitation of manganese.


2020 ◽  
Vol 33 (1) ◽  
pp. 113-119
Author(s):  
Sangeetha Parashuram Mulgund ◽  
P.B. Kalburgi ◽  
P.G. Rakaraddi

Degradation of cotton textile wastewater using Fenton’s process was investigated. The effect of individual and interactive operating parameters on the response was analyzed using central composite design (CCD), a commonly used form of response surface methodology (RSM). The operating parameters selected were pH, dosage of hydrogen peroxide, dosage of iron and the responses (dependent parameters) were chemical oxygen demand (COD) and colour. The model derived correlation coefficients R2 and R2 adj for COD were 0.982 and 0.966, respectively and the values were almost similar for colour also. The optimum values for various operating parameters namely pH, H2O2 and Fe2+ dosage were found to be 3.33, 60.57 and 1.56 mM, respectively for electrolysis time of 60 min. By performing the experiments with these values of operating parameters, the COD and colour removal efficiencies were found to be 83.5% and 98.1%, respectively.


2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Yamin Yasin ◽  
Maszlin Mohamad ◽  
Faujan B. H. Ahmad

Layered double hydroxide intercalated with tartrate (tartrate-Mg-Al) was used as an adsorbent to remove lead ions from aqueous solutions. The effects of various optimization parameters such as contact time, solution pH, lead ion concentrations, and adsorbent dosage were investigated by the use of Response Surface Methodology (RSM). The Response Surface Methodology (RSM) based on a four-level four-variable Central Composite Rotatable Design (CCRD) was employed to evaluate the interactive effects of the various optimization parameters. The parameters were contact time (6–10 h), solution pH (1–3), adsorbent dosage (0.06–0.1 g), and lead ion concentrations (10–30 mg/L). The percentage of lead ions removal for each of the parameters studied was determined by Inductively Coupled Plasma-Optical Emission Spectrophotometer. Simultaneously by increasing contact time and amount of dosage of tartrate-Mg-Al used the percentage of lead ions removal from aqueous solution will increase; however, the percentage removal decreases with an increase in pH and concentrations of lead ions. The experimental percentage removal recorded under optimum conditions was compared well with the maximum predicted value from the RSM, which suggest that Central Composite Rotatable Design of RSM can be used to study the removal of lead from aqueous solution by the use of tartrate-Mg-Al as an adsorbent.


2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.


2019 ◽  
Vol 09 ◽  
Author(s):  
Hossein Zaeri ◽  
Bahareh Kamyab Moghadas ◽  
Bijan Honarvar ◽  
Ali Shokuhi Rad

: In this research, the extraction of essential oil from Calotropis Procera with the family name of Asclepiadaceae, by supercritical carbon dioxide (CO2) solvent has been investigated in detail, and the yield and chemical profile of the extracts achieved by this method were compared with those resulted by the conventional Hydro distillation method. To optimize the process parameters of CO2 supercritical extraction (SCE) of the Calotropis Procera, the Response Surface Methodology (RSM) with central composite design (CCD) was employed. The effects of temperature, pressure, and extraction time on the oil yield are considered for investigation. Results showed that the data were sufficiently fitted into the second-order polynomial model. The extraction conditions, including pressure, temperature, and extraction time, were studied between 150-200 bar, 40-50 ºC, and 50-100 min, respectively. The optimal conditions are achieved as the temperature of 47.19ºC, the pressure of 172.2 bar, and time of 86 minutes with the retrieval rate of 31.39%.


2021 ◽  
Vol 13 (4) ◽  
pp. 2216
Author(s):  
Najeeha Mohd Apandi ◽  
Mimi Suliza Muhamad ◽  
Radin Maya Saphira Radin Mohamed ◽  
Norshuhaila Mohamed Sunar ◽  
Adel Al-Gheethi ◽  
...  

The present study aimed to optimize the production of Scenedesmus sp. biomass during the phycoremediation process. The biomass productivity was optimized using face centred central composite design (FCCCD) in response surface methodology (RSM) as a function of two independent variables that included wet market wastewater concentrations (A) with a range of 10% to 75% and aeration rate (B) with a range of 0.02 to 4.0 L/min. The results revealed that the highest biomass productivity (73 mg/L/d) and maximum growth rate (1.19 day−1) was achieved with the 64.26% of (A) and 3.08 L/min of (B). The GC-MS composition analysis of the biomass yield extract revealed that the major compounds are hexadecane (25%), glaucine (16.2%), and phytol (8.33%). The presence of these compounds suggests that WMW has the potential to be used as a production medium for Scenedesmus sp. Biomass, which has several applications in the pharmaceutical and chemical industry.


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