Identification of Salmonella Typhimurium Peptidyl-prolyl cis-trans Isomerase B (PPIase B) and Assessment of their Role in the Protein Folding

2020 ◽  
Vol 27 (8) ◽  
pp. 744-750 ◽  
Author(s):  
Manoj Kumawat ◽  
Irungbam Karuna ◽  
Neeraj Ahlawat ◽  
Sushma Ahlawat
Keyword(s):  
Protein Folding ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Vital Role ◽  
Protein Isoforms ◽  
Peptide Bonds ◽  
Cyclophilin B ◽  
Salmonella Genome ◽  
Peptidyl Prolyl Isomerases ◽  
First Time

Background: Peptidyl-prolyl cis-trans isomerase (PPIases) enzyme plays a vital role in protein folding. It catalyses the cis-trans isomerisation of peptide bonds, an essential step for newly synthesized protein to acquire its correct functional conformation in both prokaryotes and eukaryotes. Objective: The present study showed the biochemical and molecular characterisation of cyclophilins (PpiB), a type of peptidyl-prolyl isomerases proteins from the pathogenic bacteria Salmonella Typhimurium. Methods: Salmonella Typhimurium is one of the leading serovars responsible for human and animal salmonellosis globally, with the majority of human cases originating through the food chain. Here successful expression and purification of PpiB protein have been demonstrated and LC-MS based analyses showed high protein score and similarity with other PPi protein. Further the enzymatic activity of the purified recombinant PpiB was determined using Succinyl-Ala-Phe-Pro- Phe-p nitroanilide as substrate and enzyme-catalysed reaction. Result: Km and Vmax were calculated and found to be Vm = 1.023 ± .06400 min/μg, Km = 0.6219 ± 0.1701 μM, respectively. We have reported for the first time the presence of Salmonella PPIase-B (PpiB) protein isoforms in salmonella genome having PPi activity. Conclusion: Taken together, our data clearly showed that Salmonella Cyclophilin B (PpiB) protein is active and involved in diverse biological processes and highly similar to the different domain of Cyclophilin proteins.

scholarly journals Effect of Sulfonamides and Their Structurally Related Derivatives on the Activity of ι-Carbonic Anhydrase from Burkholderia territorii

2021 ◽  
Vol 22 (2) ◽  
pp. 571
Author(s):  
Viviana De Luca ◽  
Andrea Petreni ◽  
Alessio Nocentini ◽  
Andrea Scaloni ◽  
Claudiu T. Supuran ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Selective Inhibition ◽  
Extensive Study ◽  
Protein Isoforms ◽  
Carbonic Anhydrases ◽  
Physiological Processes ◽  
Microbial Survival ◽  
Reversible Hydration

Carbonic anhydrases (CAs) are essential metalloenzymes in nature, catalyzing the carbon dioxide reversible hydration into bicarbonate and proton. In humans, breathing and many other critical physiological processes depend on this enzymatic activity. The CA superfamily function and inhibition in pathogenic bacteria has recently been the object of significant advances, being demonstrated to affect microbial survival/virulence. Targeting bacterial CAs may thus be a valid alternative to expand the pharmacological arsenal against the emergence of widespread antibiotic resistance. Here, we report an extensive study on the inhibition profile of the recently discovered ι-CA class present in some bacteria, including Burkholderia territorii, namely BteCAι, using substituted benzene-sulfonamides and clinically licensed sulfonamide-, sulfamate- and sulfamide-type drugs. The BteCAι inhibition profile showed: (i) several benzene-sulfonamides with an inhibition constant lower than 100 nM; (ii) a different behavior with respect to other α, β and γ-CAs; (iii) clinically used drugs having a micromolar affinity. This prototype study contributes to the initial recognition of compounds which efficiently and selectively inhibit a bacterial member of the ι-CA class, for which such a selective inhibition with respect to other protein isoforms present in the host is highly desired and may contribute to the development of novel antimicrobials.

European Mail Armour

2021 ◽  
Author(s):  
Martijn A. Wijnhoven
Keyword(s):  
Early Modern ◽  
Middle Ages ◽  
Iron Age ◽  
Early Modern Period ◽  
Vital Role ◽  
Military Technology ◽  
Roman Army ◽  
Solid Foundation ◽  
First Time

Mail armour (commonly mislabelled 'chainmail') was used for more than two millennia on the battlefield. After its invention in the Iron Age, mail rapidly spread all over Europe and beyond. The Roman army, keen on new military technology, soon adopted mail armour and used it successfully for centuries. Its history did not stop there and mail played a vital role in warfare during the Middle Ages up to the Early Modern Period. Given its long history, one would think mail is a well-documented material, but that is not the case. For the first time, this books lays a solid foundation for the understanding of mail armour and its context through time. It applies a long-term multi-dimensional approach to extract a wealth of as yet untapped information from archaeological, iconographic and written sources. This is complemented with technical insights on the mail maker’s chaîne opératoire.

Effect of Gamma Irradiation on the Survival of Pathogens in Kwamegi, a Traditional Korean Semidried Seafood

2003 ◽  
Vol 66 (11) ◽  
pp. 2093-2096 ◽  
Author(s):  
S. P. CHAWLA ◽  
D. H. KIM ◽  
C. JO ◽  
J. W. LEE ◽  
H. P. SONG ◽  
...  
Keyword(s):  
Low Temperature ◽  
Salmonella Typhimurium ◽  
Water Activity ◽  
Pathogenic Bacteria ◽  
Radiation Sensitivity ◽  
E Coli ◽  
Public Health Significance ◽  
Irradiation Treatment ◽  
Test Organisms ◽  
Health Significance

Kwamegi (semidried raw Pacific saury) is traditional seafood available in Korea. It has water activity in the range of 0.90 to 0.95. Spoilage and the growth of most pathogenic bacteria is retarded because of low water activity, low temperature, and packaging. However, it is contaminated with bacteria of public health significance and poses a hazard to the consumer because it is consumed raw without any cooking. The effectiveness of these hurdles in preventing the growth of Staphylococcus aureus, Bacillus cereus, Salmonella Typhimurium, and Escherichia coli and the efficacy of irradiation treatment in eliminating these bacteria from kwamegi using inoculated pack studies was examined. Radiation sensitivity of S. aureus, B. cereus, Salmonella Typhimurium, and E. coli in kwamegi was investigated. D10-values of these organisms in kwamegi were 590 ± 13.6, 640 ± 14.9, 560 ± 45.4, and 550 ± 8.6 Gy, respectively. The growth of all four test organisms inoculated into these foods during 4 weeks of storage at an ambient winter temperature (ranging from −5°C to +5°C) was recorded. All four pathogens (inoculated at 106 CFU/g) were eliminated by irradiation at 4 kGy. These studies unequivocally demonstrate that irradiation, with a combination of low water activity and low temperature, results in microbiologically safe kwamegi.

scholarly journals The Diversity of Bacteria Associated with the Invasive Gall Wasp Dryocosmus kuriphilus, Its Galls and a Specialist Parasitoid on Chestnuts

Insects ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 86
Author(s):  
Xiaohui Yang ◽  
Yu Hui ◽  
Daohong Zhu ◽  
Yang Zeng ◽  
Lvquan Zhao ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Plant Pathogens ◽  
High Throughput Sequencing ◽  
Bacterial Species ◽  
Gall Wasp ◽  
Potential Vector ◽  
Dryocosmus Kuriphilus ◽  
Torymus Sinensis ◽  
Galling Insects ◽  
First Time

Dryocosmus kuriphilus (Hymenoptera: Cynipidae) induces galls on chestnut trees, which results in massive yield losses worldwide. Torymus sinensis (Hymenoptera: Torymidae) is a host-specific parasitoid that phenologically synchronizes with D. kuriphilus. Bacteria play important roles in the life cycle of galling insects. The aim of this research is to investigate the bacterial communities and predominant bacteria of D. kuriphilus, T. sinensis, D. kuriphilus galls and the galled twigs of Castanea mollissima. We sequenced the V5–V7 region of the bacterial 16S ribosomal RNA in D. kuriphilus, T. sinensis, D. kuriphilus galls and galled twigs using high-throughput sequencing for the first time. We provide the first evidence that D. kuriphilus shares most bacterial species with T. sinensis, D. kuriphilus galls and galled twigs. The predominant bacteria of D. kuriphilus are Serratia sp. and Pseudomonas sp. Furthermore, the bacterial community structures of D. kuriphilus and T. sinensis clearly differ from those of the other groups. Many species of the Serratia and Pseudomonas genera are plant pathogenic bacteria, and we suggest that D. kuriphilus may be a potential vector of plant pathogens. Furthermore, a total of 111 bacteria are common to D. kuriphilus adults, T. sinensis, D. kuriphilus galls and galled twigs, and we suggest that the bacteria may transmit horizontally among D. kuriphilus, T. sinensis, D. kuriphilus galls and galled twigs on the basis of their ecological associations.

scholarly journals First report on cyanobacterial flora from Masirah Island, Sultanate of Oman

Algologia ◽  
2020 ◽  
Vol 30 (4) ◽  
pp. 440-451
Author(s):  
M. Shamina ◽  
Keyword(s):  
Environmental Conditions ◽  
Food Industry ◽  
Vital Role ◽  
Taxonomic Composition ◽  
Climatic Conditions ◽  
Taxonomic Classification ◽  
Biofuel Production ◽  
Sultanate Of Oman ◽  
First Report ◽  
First Time

Cyanobacteria are organisms which play a vital role in various molecular and biotechnological aspects in food industry, agriculture, pharmaceuticals, neutraceuticals, biofuel production, etc., it is necessary to understand its adaptability to various environmental conditions. Furthermore it is equally important to discover new cyanobacterial taxa and with it occasional changes in taxonomic classification, thus the author set out to study cyanobacteria in extreme climatic conditions of desert, where temperatures are mostly above 45 oC. The taxonomic composition of cyanobacteria of Masirah Island, Sultanate of Oman, was studied for the first time. The studied samples were collected during the period of 2017–2019. The ten samples belonged to two orders: Oscillatoriales Schaffner and Synechococcales L.Hoffmann, Komárek & J.Kastovsky. All of them were filamentous non-heterocyst forms. Three species belonged to the genus Leptolyngbya Anagn. & Komárek, the genera Oscillatoria Vaucher ex Gomont and Lyngbya C.Agardh ex Gomont were represented by two species each, while the genera Pseudanabena Lauterborn, Planktolyngbya Anagn. & Komárek and Geitlerinema (Anagn. & Komárek) Anagn. were one species.

scholarly journals Lactic Acid Bacteria (Lactobacillus bulgaricus and Streptococcus thermophillus) in Yogurt Inhibit the Growth of Escherichia coli, Salmonella typhimurium, and Shigella sp. In Vitro

2021 ◽  
Vol 31 (4) ◽  
pp. 2
Author(s):  
IDSAP Peramiarti
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Test Method ◽  
P Value ◽  
E Coli ◽  
Significant Difference

Diarrhea is defecation with a frequency more often than usual (three times or more) a day (10 mL/kg/day) with a soft or liquid consistency, even in the form of water alone. Pathogenic bacteria, such as Escherichia coli, Salmonella typhimurium, and Shigella sp., play a role in many cases, to which antibiotics are prescribed as the first-line therapy. However, since antibiotic resistance cases are often found, preventive therapies are needed, such as consuming yogurt, which is produced through a fermentation process by lactic acid bacteria (LAB). This research aimed to determine the activity of lactic acid bacteria (Liactobacillus bulgaricus and Streptococcus thermophilus) in yogurt in inhibiting the growth of the pathogenic bacteria E. coli, S. typhimurium, and Shigella sp. The research applied in vitro with the liquid dilution test method and the true experimental design research method with post-test-only and control group design. The design was used to see the inhibitory effect of yogurt LAB on the growth of E. coli, S. typhimurium, and Shigell sp. to compare the effect of several different yogurt concentrations, namely 20%, 40%, 60%, and 80%. The results of the Least Significance Different analysis showed that there was a significant difference between yogurt with a concentration of 0% and that with various concentrations in inhibiting the growth of E. coli, S. typhimurium, and Shigella sp. with a p-value of &lt;0.05. Whereas, there was no significant difference in the various concentrations of yogurt in inhibiting the growth of the three kinds of bacteria with a p-value of &gt; 0.05.<p class="Default" align="center"> </p>

scholarly journals PD-1 Suppresses the Osteogenic and Odontogenic Differentiation of Stem Cells From Dental Apical Papilla via Targeting SHP2/NF-κB axis

2021 ◽  
Author(s):  
Na Li ◽  
Zehan Li ◽  
Lin Fu ◽  
Ming Yan ◽  
Yanqiu Wang ◽  
...  
Keyword(s):  
Vital Role ◽  
Cell Counting ◽  
Alizarin Red ◽  
Odontogenic Differentiation ◽  
Root Dentin ◽  
Apical Papilla ◽  
Effect Of Pd ◽  
First Time ◽  
The Relationship

Abstract BackgroundStem cells from the apical papilla (SCAPs) are important for tooth root development and regeneration of root dentin. Here, we examined the expression of programmed cell death protein-1 (PD-1) in SCAPs and investigated the effect of PD-1 on odontogenic and osteogenic differentiation and the relationship between PD-1 and cell differentiation and SHP2/NF-κB signals.MethodsSCAPs were obtained culture in the related medium. The proliferation ability was evaluated by cell counting kit 8 and 5‐ethynyl‐20‐deoxyuridine (EdU) assay. Alkaline phosphatase (ALP) activity assay, ALP staining, western blot, real-time RT-PCR, Alizarin Red S staining, and immunofluorescence staining were performed to explore the osteo/odontogenic potential and the involvement of SHP2/NF-κB pathways. Besides, we transplanted SCAPs component into mouse calvaria defects to evaluate osteogenesis in vivo. ResultsWe found that human SCAPs expressed PD-1 for the first time. PD-1 knockdown enhanced the osteo/odontogenic differentiation of SCAPs by suppressing SHP2 pathway and activating NF-κB pathway. Overexpression of PD-1 inhibited the osteogenesis and odontogenesis of SCAPs via activation of SHP2 signal and inhibition of NF-κB pathway. ConclusionPD-1 activated SHP2 signal to block NF-κB signal and then played a vital role in osteo/odontogenic differentiation of SCAPs.

scholarly journals CpxR regulates the colistin susceptibility of Salmonella Typhimurium by a multitarget mechanism

2020 ◽  
Vol 75 (10) ◽  
pp. 2780-2786 ◽  
Author(s):  
Ya-Jun Zhai ◽  
Hua-Run Sun ◽  
Xing-Wei Luo ◽  
Jian-Hua Liu ◽  
Yu-Shan Pan ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Target Genes ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Efflux Pump ◽  
Colistin Resistance ◽  
Mobility Shift ◽  
Serovar Typhimurium ◽  
Electrophoretic Mobility Shift Assays ◽  
First Time ◽  
Signalling Systems

Abstract Background The two-component signalling systems PmrAB and PhoPQ of Salmonella have been extensively studied with regard to colistin resistance. We previously showed that overexpressed CpxR could significantly increase the colistin susceptibility (16-fold compared with the WT strain) of Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) through PmrAB and PhoPQ. Objectives To identify the potential target genes of CpxR in PmrAB- and PhoPQ-related signalling pathways. Methods His6-CpxR was prokaryotically expressed and purified by Ni-NTA resin affinity chromatography. β-Galactosidase activity assays were conducted to investigate whether CpxR could regulate the promoters of colistin resistance-related genes (CRRGs). Electrophoretic mobility shift assays (EMSAs) were used to further detect His6-CpxR complexes with promoters of CRRGs. Results We demonstrated for the first time (to the best of our knowledge) that CpxR and the AcrAB–TolC efflux pump have reciprocal effects on CRRG transcription. Additionally, CpxR could regulate the colistin susceptibility of Salmonella Typhimurium by binding directly to the promoters of phoPQ, pmrC, pmrH and pmrD at the CpxR box-like sequences or indirectly through other regulators including pmrAB and mgrB. Conclusions CpxR could regulate the colistin susceptibility of Salmonella Typhimurium by a multitarget mechanism.

Electric features of dislocations and electric force between dislocations

2020 ◽  
pp. 108128652096564
Author(s):  
Yuanjie Huang
Keyword(s):  
Electric Field ◽  
Dielectric Materials ◽  
Dislocation Motion ◽  
Vital Role ◽  
Dislocation Dynamics ◽  
Electric Force ◽  
Fundamental Knowledge ◽  
Large Dielectric Constant ◽  
Net Charges ◽  
First Time

Dislocations and dislocation dynamics are the cores of material plasticity. In this work, the electric features of dislocations were investigated theoretically. An intrinsic electric field around a single dislocation was revealed. In addition to the well-known Peach–Koehler force, it was established that an important intrinsic electric force exists between dislocations, which is uncovered here for the first time and has been neglected since the discovery of dislocations. The electric forces may be large and sometimes could exceed the Peach–Koehler force for metals and some dielectric materials with large dielectric constant. Therefore, the electric force is anticipated to play a vital role in dislocation dynamics and material plasticity. Moreover, an external electric field could exert an electric force on dislocations and a threshold electric field was subsequently discovered above which this force enables dislocations to glide. Interestingly, it was found that some dislocations move in one direction, but others move in reverse in an identical electric field, which is in agreement with experimental observations. Despite dislocation motion under an electric field, to one’s surprise, both edge and screw dislocations do not carry net charges by themselves, which may tackle the long-standing puzzle on the charges of dislocations. These findings may supply people with new fundamental knowledge on dislocations as well as dislocation dynamics, and may assist people in understanding related phenomena.

Salmonella Genomic Island 1 (SGI1) and genetic characteristics of animal and food isolates of Salmonella Typhimurium DT104 in Hungary

2008 ◽  
Vol 56 (1) ◽  
pp. 5-11 ◽  
Author(s):  
Péter Fekete ◽  
Béla Nagy
Keyword(s):  
Salmonella Typhimurium ◽  
Gel Electrophoresis ◽  
Animal Species ◽  
Genomic Island ◽  
Phage Type ◽  
Geographic Origin ◽  
Pulsed Field Gel Electrophoresis ◽  
Animal Origin ◽  
Genetic Characteristics ◽  
First Time

To study the genetic characteristics of DT104 strains of Salmonella Typhimurium and the prevalence of Salmonella Genomic Island (SGI1) in Hungary, 140 recent Salmonella strains of food and animal origin were examined. For the first time in Hungary, the SGI1 was found in 17 out of 59 S . Typhimurium isolates (all proven to be DT104 phage type). These 17 strains were then subtyped by pulsed-field gel electrophoresis (PFGE) into 6 pulsotypes which were less correlated with the geographic origin than with the animal species of origin.

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