scholarly journals effectiveness testing of l-carnitine on the quality of spermatozoa and testosterone hormone in white rats (Rattus norvegicus) feeding with high fat

2021 ◽  
Vol 4 (1) ◽  
pp. 102-109
Author(s):  
Ni Wayan Sukma Antari ◽  
Ida Ayu Manik Damayanti ◽  
Nadya Treesna Wulansari
Keyword(s):  
Rattus Norvegicus ◽  
Membrane Integrity ◽  
White Rats ◽  
Long Time ◽  
Statistical Program ◽  
High Doses ◽  
The One ◽  
Dose Variation ◽  
One Way Anova

This study aims to determine the increase in the quality of spermatozoa and testosterone after giving L-carnitine to white rats (Rattus norvegicus). This study was conducted by giving L-carnitine as a treatment for 42 days in white rats with a dose variation of 100 mg/kg bw, 150 mg/kg bw, and 200 mg/kg bw and user controls as a comparison. The variables observed in this study were the quality of spermatozoa, namely: morphology, motility, viability, membrane integrity, and seeing the levels of the hormone testosterone. The research data were processed using a computer statistical program (SPSS 22.0 for Windows) using the One Way Anova test. The results showed that giving high doses of L-carnitine supplements for a long time could cause decreased spermatozoa quality, namely: morphology, motility, viability, membrane integrity and decreased levels of the hormone testosterone.

scholarly journals PROVISION OF ARAK BALI REDUCES SPERMATOZOA QUALITY OF WHITE RATS (Rattus norvegicus)

2017 ◽  
Vol 52 (4) ◽  
pp. 235
Author(s):  
Ni Wayan Sukma Antari ◽  
Alfiah Hayati ◽  
Dwi Winarni
Keyword(s):  
Phase Ii ◽  
Rattus Norvegicus ◽  
Membrane Integrity ◽  
Male Rats ◽  
Experimental Animals ◽  
Alcohol Synthesis ◽  
White Rats ◽  
Two Phases

This study aimed to determine the effect of arak bali on the quality of spermatozoa include morphology, motility, viability, membrane integrity of spermatozoa rat (Rattus norvegicus). The study was conducted in two phases: the first phase of the deployment of questionnaires conducted in five districts in Bali to determine the type and frequency of arak bali consumption and phase II made arak bali giving treatment in experimental animals. This study used 24 male rats (170-200 grams), divided into four groups: one control and three treatments (by arak bali containing 40% alcohol as much as 0.1 and 0.5 mL and 0.1 mL much alcohol synthesis, for 45 days. the results showed that of the five districts in Bali, most people consume arak bali commercial and most of the frequency of consumption of the week more than one bottle (350 mL). the provision of arak bali in experimental animals, degrade the quality (morphology, motility, viability, membrane integrity), the greater the volume given declining spermatozoa quality.

scholarly journals POTENSI PEMBERIAN EKSTRAK BUAH DELIMA (Punica granatum L) TERHADAP KUALITAS SPERMATOZOA TIKUS PUTIH (Rattus norvegicus) YANG TERPAPAR PANAS

2020 ◽  
Vol 8 (1) ◽  
pp. 5
Author(s):  
Nucifera Fadhillah Santoso ◽  
Budi Utomo ◽  
Mirni Lamid
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Membrane Integrity ◽  
Punica Granatum ◽  
Control Treatment ◽  
Control Group ◽  
White Rats ◽  
Significant Difference ◽  
The Difference

The purpose of this research was to determine the effect of pomegranate (Punica granatul l) fruit extract for spermatozoa quality including the motility, viability and membrane integrity of spermatozoa. The researchused 20 male white rats  (Rattus norvegicus) with 200-300 gram average body weight. P0 as control was given with CMC Na 0.5%.  P1 used 75 mg/kg BW dose of extract,  P2used 150 mg/kg BW dose of extract,  P3 used 300 mg/kg BW dose of extract. The treatment was given perorally for 14 days. The experiment was used completely random design (CRD). The result of this study was analyzed with ANOVA followed by Tukey Test (p<0.05). The result showed that there was significant effect from therapy on the quality of rats spermatozoa. The difference of spermatozoa’s concentration between first treatment group P1 and control treatment group is not significant, while the treatment other result showed between the second treatment group and the control group P0. A significant difference occurs in the percentage of motility, viability and  membrane integrity. The treatment from P2 group showed the best result in maintaining the quality of rats spermatozoa.

scholarly journals PENGARUH PEMBERIAN INFUSA DAUN LABU SIAM (Sechium edule) TERHADAP PENURUNAN KADAR KOLETEROL DARAH TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 151
Author(s):  
Ausich Singal ◽  
Edwin De Queljoe ◽  
Paulina Yamlean
Keyword(s):  
Rattus Norvegicus ◽  
Dose Group ◽  
T Test ◽  
Blood Cholesterol ◽  
Control Group ◽  
Cholesterol Levels ◽  
White Rats ◽  
Sechium Edule ◽  
One Way Anova ◽  
Paired T Test

ABSTRACT This study aims to determine the effect of infusion of conjoined pumpkin leaves (Sechium edule) on reducing total blood cholesterol of male white rats (Rattus norvegicus). The subjects of this study were 15 male white rats with an average body weight of 200 grams which were divided into 5 groups, each group consisted of 3 mice. The method used is a laboratory experiment with a completely randomized design. The results were obtained from 2 measurements of blood cholesterol levels, namely measurements before and after treatment. The treatment begins with the provision of high-fat foods for 48 days. On the 49th day a blood cholesterol level was measured before treatment. Furthermore, treatment was given to each group, namely aquades in the negative control group, simvastatin in the positive control group, and squash leaves infusion with their respective doses in the dose group I (40%), the dose group II (20%), and the dose group III (10%). Measurement of cholesterol levels after treatment was carried out on day 54. Data were analyzed by Paired t-test and One Way ANOVA. The analysis showed that there were no significant differences between treatment groups. Judging from the change in average and percentage, 40% infusion dose of siamese pumpkin leaves gives the best reduction in cholesterol levels. Keywords: Cholesterol, pumpkin leaves, male white mouse infusion. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian infusa daun labu siam (Sechium edule ) terhadap penuruan kolesterol darah total tikus putih jantan (Rattus norvegicus). Subjek penelitian ini berupa tikus putih jantan berjumlah 15 ekor dengan berat badan rata-rata 200 gram yang dibagi dalam 5 kelompok, masing-masing kelompok sebanyak 3 ekor. Metode yang digunakan yaitu eksperimen laboratorium dengan rancangan acak lengkap. Hasil penelitian diperoleh dari 2 kali pengukuran kadar koleterol darah yaitu pengukuran sebelum dan sesudah perlakuan. Perlakuan dimulai dengan pemberian makanan tinggi lemak selama 48 hari. Pada hari ke49 dilakukan pengukuran kadar kolesterol darah sebelum perlakuan. Selanjutnya diberikan perlakuan pada tiap kelompok yaitu aquades pada kelompok kontrol negatif, simvastatin pada kelompok kontrol positif, dan infusa daun labu siam dengan dosis masing-masing pada kelompok dosis I (40 %), kelompok dosis II (20 %), dan kelompok dosis III (10%). Pengukuran kadar kolesterol sesudah perlakuan dilakukan pada hari 54. Data diananlisis dengan Paired t-test dan One Way ANOVA. Hasil analisa menunjukan tidak terdapat perbedaan yang bermakna antar kelompok perlakuan. Dilihat dari perubahan rerataan dan presentase, dosis infusa  40% daun labu siam memberikan penurunan kadar koleterol terbaik. Kata kunci : Infusa daun Labu siam, kolesterol, tikus putih jantan.

scholarly journals The Effect of Balimo (Zanthoxylum nitidum) Immersion Water On The Hematological Profile of White Rats (Rattus norvegicus) That Given Liquor (Ciu)

2021 ◽  
Vol 10 (1) ◽  
pp. 47-57
Author(s):  
Panca Buana Wijaya ◽  
Tyas Rini Saraswati ◽  
Silvana Tana ◽  
Sunarno Sunarno ◽  
Erma Prihastanti
Keyword(s):  
Rattus Norvegicus ◽  
Male Rats ◽  
Excessive Alcohol Consumption ◽  
White Rats ◽  
Excessive Alcohol ◽  
The Mean ◽  
Zanthoxylum Nitidum ◽  
The Difference ◽  
Antioxidant Effects ◽  
One Way Anova

Consumption of liquor such as Ciu in excessive doses can cause a decrease in hematological status. Balimo stem is an alternative treatment to improve hematological status due to excessive alcohol consumption because it contains alkaloids, flavonoids, and other secondary metabolic compounds, that have functions as antioxidant effects. This study aims to examine and analyze the effect of Balimo immersion water on the hematological status of mice with the observed variables, namely the erythrocytes count, hemoglobin levels, hematocrit value, and total count of leukocytes in rats that had been given Ciu. The study used 20 Rattus norvegicus male rats which were divided into 4 groups. The data were analyzed using one-way ANOVA. The results showed no significant differences (p <0.05) on the Balimo immersion water treatment, but if it was seen from the difference in the mean data of each variable, it could still be seen the difference from each treatment. In this study, it can be concluded that Balimo immersion water was able to improve the hematological status of rats that had been given Ciu liquor with a 0,2 mL dose.

scholarly journals Improving the Quality of Oocytes of Old and Productive Ages White Rats (Rattus norvegicus) Using Pregnant Mare Serum Gonadotrophin

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Sultania A Suleman ◽  
Andriyanto Andriyanto ◽  
Aulia Andi Mustika ◽  
Wasmen Manalu
Keyword(s):  
Rattus Norvegicus ◽  
Oocyte Quality ◽  
The Other ◽  
Female Rats ◽  
Age Group ◽  
White Rats ◽  
Maze Test ◽  
Swimming Test ◽  
Pregnant Mare Serum Gonadotrophin

Abstract This study was conducted to improve the quality of oocytes in old-female rats by using pregnant mare serum gonadotrophin (PMSG). Female rats at productive age were used as a control.  The experimental rats were injected with 4 doses of PMSG i.e., 0, 2.5, 5.0, and 10 IU PMSG. After 2 weeks of acclimation to the experimantal condition, the experimental rats were injected with PGF2α at a dose of 25µg/g BW two times with 2 days interval to synchronize estrous cycle. PMSG injections were conducted at the same time with the second PGF2α injection.  After PMSG injection, the experimental rats were divided into two groups rats, i.e., rats without mating for masurement of oocyte qualities and rats mated for measurement of offspring qualities.  Therefore, 16 experimental rats from each age group were sacrifized for maeasurement of oocyte qualities.  The other 16 experimental rats for each age group were mated for measurement of offspring qualities. Parameters measured were hematological profile, uterine and ovarian weights, the qualities of oocytes, the qualities of the offsspring born by using swimming test and rat maze test. The collected data were analyzed by analysis of variance (ANOVA) and continued with Duncan test with a 95% confidence level. The data were analyzed using SSPS. The results showed that the improvement in the quality of oocytes in old female rats using the PMSG hormone in this study showed an increase in the quality of oocytes in old and productive age rats. The highest number of oocyte quality was found in rats of productive age (3.25) and significantly different (P< 0.05) from the other oocyte qualities. The quality of offspring born to old age and productive age rats injected with PMSG were improved.  It was concluded that the improvement of oocyte quality by using PMSG also improves offspring qualities.

REGIONAL INEQUALITIES OF CHILDREN DISABILITY INCIDENCE IN LITHUANIA

2015 ◽  
Vol 25 (1) ◽  
pp. 33-38
Author(s):  
Juozas Raistenskis ◽  
Zdislav Skvarciany ◽  
Romualdas Gurevičius
Keyword(s):  
Variation Analysis ◽  
Related Factors ◽  
The Social ◽  
Statistical Program ◽  
The Republic ◽  
The One ◽  
First Time ◽  
One Way Anova ◽  
Direct Standardization

Declining birth rates, increasing number of diseases and injuries among children, socio-economical change in the situation of the country, as well as other social, health - and economics-related factors are the main reasons for greater interest of scientists in research on the social and health disparities in the counties (municipalities and districts). This paper analyzes the inequalities in first-time recognized children’s disability in counties of Lithuania during the period of 2007-2011. Aim of the study. To analyze incidences of the firsttime recognized children’s disability in Lithuania from 2007 to 2011 and to compare the parameters of the first-time recognized children’s disability incidence in 10 counties, taking into account variability of disability incidence inside the counties. Methods. The study used the data on the initial determination of disability in children, taken from Disability and Working Capacity Assessment Office under the Ministry of Social Security and Labor from 2007-2011. For the usage of further statistical index, the information on the number of citizens was taken from the database of Department of Statistics of the Republic of Lithuania. The indicators were age-standardized using the direct standardization method and the statistical program “WinPepi”. For the variation analysis of country’s regional disparities the One-way ANOVA test was applied. Results. During the analyzed period (2007-2011) in Lithuania some form of disability was found in 10649 children under the age of 18, of whom 6,219 - boys (58,0 percent) and 4,430 - girls (42,0 percent). The largest newly-recognized children’s disability averages in 2007-2011 were found in the region of Šiauliai (43,68 ± 1,70), Panevėžys county (41,55 ± 1,96) and Utena county (41,53 ± 6,27). In boys’ group - Šiauliai county (51,64 ± 1,53), Telšiai county (47,72 ± 10,61), Utena county (46,69 ± 7,83) and Panevėžys county (45,61 ± 2,59). In girls’ group - Panevėžys (37,14 ± 2,31 ), Šiauliai (35,33 ± 2,47), and Utena (36,16 ± 5,11) c o unties. In both girls’ and boys’ groups statistically significant differences (p0.05) in newly recognized children’s disability incidence in the country’s counties were found. Conclusions. The conducted variation analysis of children’s disability at t h e age of 0-17 y.o. in the period of 2007-2011 throughout the country’s counties, revealed inequalities among the counties. The biggest differences in newly determined disability in children in comparison with other counties of the country were found among Šiauliai, Utena, and Panevėžys counties.

scholarly journals UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 572
Author(s):  
Asriyani Samiun ◽  
Edwin De Queljoe ◽  
Irma Antasionasti
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Male Rats ◽  
Test Material ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain ◽  
One Way Anova ◽  
Vernonia Cinerea ◽  
Dpt Vaccine

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit  (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit  setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan

Improving the Qualified Rate of Flux Test about Scavenging Pump in Aeroengine by Applying Six Sigma Methodology

2010 ◽  
Vol 26-28 ◽  
pp. 289-292
Author(s):  
Yang Hua Cao ◽  
Xiu Cui Kang
Keyword(s):  
Six Sigma ◽  
Influence Factors ◽  
Assembly Quality ◽  
Low Level ◽  
Long Time ◽  
Improvement Measures ◽  
Six Sigma Methodology ◽  
The One ◽  
Main Influence

The qualified rate of flux test is decided mainly by the assembly parameters, but for some reasons, the qualified rate especially the one-turn qualified rate had been on a low level for a long time. By applying 6σ methodology, this paper analyzed the influence factors about the flux test and found out the main influence factors firstly; then on base of these analysis results, this paper proposed some improvement measures; finally, this paper validated the effects of these improvement measures by carrying out some tests. The improvement measures proposed in this paper had improved the assembly quality of scavenging pump obviously.

scholarly journals SCIENTIFIC PRODUCTION AND SCIENTIFIC QUALIFICATION EVALUATION: SOME PROBLEMATIC ISSUES

2011 ◽  
Vol 3 (1) ◽  
pp. 8-18
Author(s):  
Vincentas Lamanauskas
Keyword(s):  
Academic Staff ◽  
Scientific Activity ◽  
Scientific Production ◽  
Education Quality ◽  
Long Time ◽  
The One ◽  
Level Of Motivation ◽  
Qualification Evaluation ◽  
Scientific Pedagogical

In recent years, there has been a lot of discussion about the quality of studies and, in general, about the education quality. As we know, the quality of studies depends not only on level of motivation and cleverness of students, how well developed the infrastructure of institutions is and so on. Probably, even more, the quality of studies depends on scientific pedagogical personnel, the one, which directly determines the preparation of a future student. This article deals with problems related to the scientific production and scientific qualification evaluation. Also the article describes some characteristics of researchers and scientific production in general. Science, in some sense, has strong immunity, but in recent years, it has been rapidly weakened both internally and externally. Of course, we have to believe, that universities will understand that it is firstly on them to save and strengthen both quality of activities and prestige and develop real but not demonstrated science. Europe “has been having a headache” for a long time thinking how to withstand ever - strengthening competition with such regions as North America (especially USA), South-east Asia and so on. Key words: scientific activity evaluation, academic staff, scientific production, universities.

scholarly journals Zinc, calcium, protein, lead, mercury, and the sensorics quality of cireng snacks supplemented with blood cockle (Anadara granosa)

2017 ◽  
Vol 9 (4) ◽  
pp. 385-391
Author(s):  
MARGARETHA SOLANG ◽  
DJUNA LAMONDO ◽  
SYAM S. KUMAJI
Keyword(s):  
Economic Value ◽  
National Standard ◽  
Anova Test ◽  
Nutrition Value ◽  
The One ◽  
Tapioca Flour ◽  
Blood Cockle ◽  
Anadara Granosa ◽  
One Way Anova

Solang M, Lamondo D, Kumaji SS. 2017. Zinc, calcium, protein, lead, mercury, and the sensorics quality of cireng snacks supplemented with blood cockle (Anadara granosa). Nusantara Bioscience 9: 385-391. Blood cockle (Anadara granosa) is a potential nutritious food with high economic value. This study aims at evaluating the level of zinc, calcium, protein, mercury, lead, and the sensorics quality of cireng (traditional Indonesian snack made from fried-tapioca flour dipped into sauces) supplemented with blood cockle. This study used complete randomised design, where the treatment factors consisted of flour made from blood cockles to supplement the flour used in making the cireng snacks. The concentration of the treatment factors were 0%, 5%, 10%, 15%, and 20%. The data were analyzed using the One Way ANOVA test and LSD test. This study shows that blood cockles’ supplementation significantly increases the level of protein (p=0.05), zinc (p=0.031), calcium (p= 0.016), lead (0.000), mercury (p= 0.022) of the cireng snacks. Supplementation of blood cockles has increased the preference toward cireng’s flavor, aroma, and color by 10%, whereas the level of preference toward the texture of cireng which used the blood cockle has increased by 20% compared to cireng product with non-supplemented flour. The level of lead (Pb), and Mercury (Hg) are below the Indonesian National Standard (SNI). Supplementation of blood cockles from Gorontalo in the flour used in making the cireng snacks produced snacks with a better nutrition value and safe to consume. Also, the flavor, color, texture, and aroma are acceptable.

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