Effect of Indole-3-butyric Acid and Maturity Level of Single Node Stem Cuttings on Propagation of Exacum ritigalensis

A study was conducted to observe the effect of different concentrations of indole-3-butyric acid (IBA) and maturity levels of single node stem cuttings for vegetative propagation ofE. ritigalensis. Branches of the plant were treated by 20% Clorox in 20 minutes and rinsed it two times using distilled water. Stems cuttings weregrouped into three according to the diameter (20-30 mm, soft wood; 30-40 mm, semi hardwood and 40-50 mm, hardwood).Three differentways of leaves remaining in single node stem cuttings were used; half of single leaf, half double opposite leaves and without leaf. Stem cuttings were treated by IBA (0, 2, 2.5, 5, 10 and 15 mgL-1) for 15 minutes.Plant propagators were prepared by poly bags (150 gauges) and filled by sterilized coir dust. Thereafter, they were kept in two months and observed the survival rates of cuttings (greenish or not),number of roots,length of the lengthiest root, number of buds and number of leaves per bud. Experiments were arranged in three factor factorial completely randomized design (FCRD) with ten replicates. Statistical analysis was performed with Duncan’s multiple range test using SAS software (version 9.1.3). It was observed that rooting was at initial stage of all cuttings after one month. Result were showed that the semi hardwood single node stem with half single leaf or double leavestreated by 10 mgL-1 and 15 mgL-1 IBA were best for rooting compare to other all treatments and control, showed 100% survival rate with three buds per cutting.

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Growth and Yield of Tomato (Lycopersicon esculentum Mill.) as Influenced by Poultry Manure and Biochar in Two (2) Soil Depths

Journal of Experimental Agriculture International ◽

10.9734/jeai/2020/v42i330484 ◽

2020 ◽

pp. 55-63

Author(s):

F. B. Musa ◽

F. O. Abiodun ◽

A. R. Falana ◽

B. H. Ugege ◽

R. V. Oyewumi ◽

...

Keyword(s):

Plant Height ◽

Poultry Manure ◽

Botanical Garden ◽

Growth And Yield ◽

Tomato Plants ◽

Randomized Design ◽

Significant Difference ◽

Number Of Leaves ◽

Collar Diameter ◽

And Control

Background and Objective: Unproductive soil has been a major problem to farmers, recent interest in the use of a stable organic manure (biochar) as soil amendment and poultry manure to increase soil fertility is also increasing. Hence, the experiment was conducted to assess the condition of poultry manure and biochar on the growth and yield of tomato. Materials and Methods: A greenhouse experiment was conducted at the Federal College of Forestry Ibadan, using polyethylene bag with 5 kg soil collected at two (2) soils depths, 0-15 cm and 15-30 cm depth at the botanical garden, Federal College of Forestry Ibadan. The experiment was arranged in a completely randomized design (CRD) with four replicates. The treatments used were poultry manure (5t/ha), biochar (10t/ha), mixture of poultry manure and biochar (1:1) and control (no amendment).The tomato seeds (UC82B) were sourced from NIHORT, Ibadan and raised in a germination basket. Data were collected weekly on plant height, collar diameter and number of leaves, 50% days to flowering and yield of tomato plants from two weeks after transplanting (WAT). Data collected were analyzed statistically using ANOVA while means were separated using LSD (p<0.05) where applicable. Results: In 0-15 cm soil, the growth variables (plant height, number of leaves and collar diameter) increased with various treatments used. The highest tomato height was observed with the application of biochar (10t/ha). All the treatments applied affected the number of tomato leaves at all weeks after transplanting (WAT) with poultry manure 5t/ha having the highest number of leaves at 7WAT, while collar diameter was significantly affected by various treatments from 2 to 5 WAT, with the mixture of P.M + BIO (1:1) recording the highest value and the control plot recorded the least values in all weeks. However, in 15-30 cm soil, there was significant difference in the number of leaves of tomato among all the amended soil from 5 to 7 WAT, the highest tomato leaves were obtained when the soil was amended with poultry manure (5t/ha) and can be compared with other treatments. Interaction of poultry manure and biochar(1:1) and poultry manure 5 t/ha resulted in highest height of tomato plant from 3-7 WAT as compared to the control that had the least value but were not significantly different from the sole application of biochar 10t/ha. Interaction of poultry manure + biochar (1:1) and poultry manure (5t/ha) gave the highest tomato flowering and fruits. Conclusion: Based on this result, biochar and poultry manure when added to soil, boosted the mineralization of the soil after amendment.

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Effect of date of planting on the growth and leaf yield of Stevia (Stevia rebaudiana)

Journal of the Bangladesh Agricultural University ◽

10.3329/jbau.v10i2.14684 ◽

2013 ◽

Vol 10 (2) ◽

pp. 205-210

Author(s):

AR Khan ◽

SH Chowdhury ◽

MM Karim

Keyword(s):

Stevia Rebaudiana ◽

Dry Weight ◽

Growth And Yield ◽

Stem Cuttings ◽

Fresh Weight ◽

Planting Material ◽

Yield Parameters ◽

Randomized Design ◽

Leaf Yield ◽

Number Of Leaves

An experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh from December 2009 to March 2011 to determine the effect of date of planting on growth and leaf yield of Stevia. The experiment comprised 24 dates of planting in the year of 2010 viz. 1 January (T1), 15 January (T2), 1 February (T3), 15 February (T4), 1 March (T5), 15 March (T6), 1 April (T7), 15 April (T8), 1 May (T9), 15 May (T10), 1 June (T11), 15 June (T12), 1 July (T13), 15 July (T14), 1 August (T15), 15 August (T16), 1 September (T17), 15 September (T18), 1 October (T19), 15 October (T20), 1 November (T21), 15 November (T22), 1 December (T23) and 15 December (T24). The experiment was laid out in a Complete Randomized Design with three replications. Twenty four dates of planting were included in the experiment in 72 earthen pots (24 X 3) of 30 cm diameter X 30 cm height. Stem cuttings were placed in shaded seed bed for root initiation. Twenty one - day old stem cuttings were used as planting material. Stem cuttings were transplanted in the pots on the specific dates. Different dates of planting had significant effect on the growth and yield parameters. 1 April 2010 (T7) planting proved to be the superior to the other treatments i.e. plant height (68.50cm), number of leaves plant-1 (142.33), number of primary branches plant-1 (37.33), fresh weight of leaves plant-1 (14.11g), fresh weight of primary and secondary branches plant-1 (11.06g and 20.91g), dry weight of leaves plant-1 (3.38g) which might be due to warm environmental conditions like clear sunshine and high temperature. The overall results suggested that stevia seedlings may be planted from 15 February to 30 April for better yield of leaves. DOI: http://dx.doi.org/10.3329/jbau.v10i2.14684 J. Bangladesh Agril. Univ. 10(2): 205-210, 2012

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Effect of Hormones (IBA & IAA) on the Propagation of Himalayan Yew in Pakistan: A Conservation Approach

10.21203/rs.3.rs-27841/v1 ◽

2020 ◽

Author(s):

Javaid Iqbal ◽

Bushra Khan ◽

Sardar Khan ◽

Nasreen Ghaffar ◽

Ishaq Ahmad Mian ◽

...

Keyword(s):

Acetic Acid ◽

Medicinal Plants ◽

Butyric Acid ◽

Indole Acetic Acid ◽

Average Length ◽

Stem Cuttings ◽

Indole Butyric Acid ◽

Number Of Leaves ◽

Himalayan Yew ◽

Taxus Wallichiana

Abstract Background Himalayan yew (Taxus wallichiana) is one of the endangered medicinal plants species having great importance due to the presence of anticancer drug Taxol. This metabolite is mainly used for the treatment of ovarian, breast, AIDS-related cancers, and other indications. The study being reported here was conducted for the propagation of Himalayan yew by using two different trials of Indole Butyric acid (IBA) and Indole acetic acid (IAA) hormones treatments (2000–7000 ppm) through stem cuttings (140 each). In the same way, 3840 cuttings were treated with IBA and IAA from November 2016 to November 2017 at Lalku valley, Swat, Khyber Pakhtunkhwa (KP), Pakistan. Results The influence of IBA treatment (7000 ppm) showed a survival of 85.22% (average number of roots = 10.4, average length of roots = 15.5 cm, average number of leaves = 92.4 and average number of sprouts = 3.3) while that of IAA treatment (7000 ppm) the survival of 81.11% (average number of roots = 9.1, average length of roots = 14.6 cm, average number of leaves = 84.0 and average number of sprout = 3.0) were more significant followed by 2000–6000 ppm (IBA and IAA). Lowest survival 40–45% (average number of roots = 4.2, average length of roots = 8.0 cm, average number of leaves = 32.2, average number of sprouts = 1.7) was noticed for controlled cuttings. Conclusion The present study enhanced the potential of conservation and propagation of T. wallichiana. Hence our study suggests and recommends the application of IBA (7000 ppm) as a better hormone for the conservation and propagation of Himalayan yew.

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Indole -3- Butyric Acid Induces Plant Regeneration From Stem Cuttings Of Three Medicinal Plants

Nigerian Journal of Biotechnology ◽

10.4314/njb.v37i1.12 ◽

2020 ◽

Vol 37 (1) ◽

pp. 109-121

Author(s):

C.U. Okafor ◽

E.U. Njoku ◽

F.C. Ike ◽

C.C. Onyekwuluje

Keyword(s):

Medicinal Plants ◽

Butyric Acid ◽

Survival Rates ◽

Control Treatment ◽

Stem Cuttings ◽

Root Number ◽

Growth And Survival ◽

Root Numbers ◽

Significant Difference ◽

Leaf Formation

Field regeneration of three (3) medicinal plants - Securidaca longepedunculata (violet tree), Ocimum gratissimum (scent leaf) and Pterocarpus mildebraedii by means of rooting of stem cuttings at different lengths of 10 -15 cm and 15 -20 cm were studied. The effect of the plant hormone Indole-3-Butyric acid (IBA) at 5g/l was tested on rooting, bud sprout and leaf formation of the rooted stem cuttings of the plant species as well as the effect of the length of the stem cuttings on their growth and survival rates. The results of the study revealed that stem cuttings of O. gratissimum treated with IBA and the control treatment sprouted within 5 - 8 days. The treated O. gratissimum stem cuttings produced a slightly higher number of buds (2.58 ± 0.86) when compared to the control (2.00 ± 0.89) with no significant difference at P ≤ 0.05. Stem cuttings of the treated P. mildbraedii cuttings sprouted within 8 -10 days with 1.40 ± 0.37 number of buds, with the control showing no sprouts. Consequently, IBA application produced more leaves (13.00 ± 5.58) and roots (1.33 ± 0.01) than the control. However, O. gratissimum stem cuttings had the highest leaf (13.08 ± 4.47) and root numbers (135.00 ± 13.45) followed by the control with values- 13.00 ± 5.58 for leaf development and 61.66 ± 2.34 for root number respectively. Irrespective of the treatments, none of the stem cuttings of S. longepedunculata sprouted. This study showed that exogenous application of IBA to stem cuttings of the tested plants, except S. Longepedunculata, improved the root number, number of leaves and number of buds. Keywords: Stem cuttings, Indole-3-Butyric acid (IBA), Rooting

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Pengaruh Pemberian Berbagai Konsentrasi Indole-3-Butyric Acid (IBA) dan Lama Perendaman terhadap Pertumbuhan Stek Batang Timi (Thymus vulgaris L.)

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v23i1.44802 ◽

2021 ◽

Vol 23 (1) ◽

pp. 19

Author(s):

Khodijah Wafia ◽

Karno Karno ◽

Florentina Kusmiyati

Keyword(s):

Dry Weight ◽

Agricultural Science ◽

Thymus Vulgaris ◽

Stem Cuttings ◽

Root Dry Weight ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Screen House ◽

Plant Length

The method of thyme stem cutting needs to be developed to produce better roots to support growth. The study aims to examine the response of thyme stem cuttings to various concentration of IBA and time soaking. The research was carried out on January – March 2020 in screen house PT. INDMIRA, Sleman, Yogyakarta and the Physiology and Plant Breeding Laboratory of the Faculty of Animal and Agricultural Science, Diponegoro University. The method used was a completely randomized design with a factorial 4×3 with 5 replications. The first factor was a various concentration of IBA: A0 (0 ppm), A1 (100 ppm), A2 (250 ppm), and A3 (500 ppm). The second factor was the time soaking: B1 (5 minutes), B2 (10 minutes), and B3 (15 minutes.) The results showed that IBA concentration has no significant effect for root dry weight, while the time soaking has significant effect for all parameters. Soaking thyme stem cuttings at an IBA 250 ppm for 10 minutes gave the best results on plant length, the number of leaves, root length, and root dry weight, but soaking in water (without IBA) for 5 minutes was able to give the best number of percentage of life cuttings (85%) so that giving IBA had no needed.

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Phenotipical Characters of Melon (Cucumis melo L.) in Response to Karst Critical Land

BIOTROPIC The Journal of Tropical Biology ◽

10.29080/biotropic.2018.2.1.1-10 ◽

2018 ◽

Vol 2 (1) ◽

pp. 1-10

Author(s):

Yuanita Rachmawati ◽

Budi Setiadi Daryono ◽

Ganies Riza Ariestya

Keyword(s):

Water Content ◽

Plant Height ◽

Research Result ◽

Control Plant ◽

Fruit Weight ◽

Phenotypic Characters ◽

Randomized Design ◽

Number Of Leaves ◽

And Control ◽

Cucumis Melo L

Yogyakarta Agroecosystem has 158,600 ha of critical land spread over three zones. Two areas are Karst Land, located on Agroecosystem II includes Gunungsewu Hills, Gunungkidul and III covers Dlingo Bantul Hills and Sentolo Hills Kulon Progo Regency.. Karst Land is certainly provides stress to plants. These research purposes are examining the phenotype character of superior melon Cultivar TACAPA compare to parents and offsprings phenotypes. The phenotype characters are based on plant height, leaf number, time of melon flowering, water content of plants, and fruit and seed productivity. This experiment was done by Split Plot Design with Completely Randomized Design (CRD) with 4 kinds of treatment (control plant media, Gunungsewu, Dlingo, and Sentolo), 7 experimental units cultivars: TACAPA, TA, TP, PT, AT, Action 434, PI 371795), and 4 replications. Research result reveals that most of the phenotypic characters including plant height, number of leaves, fruit weight, and number of seeds produced have relatively no significant effect between treatment and control, while the phenotypic first time flowering time and water content of the plant, have a noticeable difference.

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Effect of hormone on the seed germination of Garcinia kola Heckel.

Journal of Agriculture Forestry and the Social Sciences ◽

10.4314/joafss.v11i2.26 ◽

2015 ◽

Vol 11 (2) ◽

pp. 223-231

Author(s):

FB Yakubu ◽

JO Ogunade ◽

OT Bolanle-Ojo ◽

DK Yahaya

Keyword(s):

Seed Germination ◽

Early Growth ◽

Garcinia Kola ◽

Randomized Design ◽

In The Wild ◽

Number Of Leaves ◽

Collar Diameter ◽

Completely Randomized Design ◽

Garcinia Kola Heckel ◽

And Control

Garcinia kola plays an important socioeconomic role in the Africa culture. Inspite of this, the plant only exists in the wild or as part of left over in the Agroforestry farms. Therefore, this research examined the phenology in seed germination for possible plantation establishment. G. kola seed dormancy and growth was investigated using hormone Napthalene Acetic Acid (NAA) and Benzene Amino Purine (BAP) application to induce quick germination and early growth The experiment was laid in a Completely Randomized Design (CRD) with seven treatments (0.5mg/ml BAP, 0.5mg/ml NAA, 1mg/ml BAP, 1mg/ml NAA, 2mg/ml BAP, 2mg/ml NAA and Control) in twenty replications. The results showed that Garcinia kola seeds germinated in seven weeks in IBA. Growth were assessed for 19wks and the result showed that Length of Radicle (LR) and Number of Radicle (NR) were significantly affected by the treatments, while 0.5mg/ml IBA gave the best result among the treatments. Length of Plumule, Number of Leaves and Collar diameter were not significantly affected by the treatments.Keywords: Garcinia kola, seed, hormone, dormancy, germination

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Use of microbial inoculants for production of potato tuber seed in greenhouse conditions

Peruvian Agricultural Research ◽

10.51431/par.v1i2.581 ◽

2020 ◽

Vol 1 (2) ◽

Author(s):

H. Torres-Vite ◽

S. E. Contreras-Liza

Keyword(s):

Bacillus Subtilis ◽

Glomus Intraradices ◽

Average Weight ◽

Potato Seed ◽

Agronomic Performance ◽

Microbial Inoculants ◽

Randomized Design ◽

Number Of Leaves ◽

And Control

Objectives: To determine the effect of Glomus intraradices and Bacillus subtilis on the agronomic performance from in vitro potato seedlings in greenhouse conditions. Methodology: The research was developed in pots under greenhouse conditions at Universidad Nacional José Faustino Sánchez Carrión, Huacho, Lima, from October 2017 to March 2018. The substrate for pots was 45% sand, 45% vermicompost, and 10% rice straw, with an average content of 3% organic matter. The average environmental temperature was 25°C. In vitro seedlings of five potato genotypes were transplanted into 4-liter pots, and inoculated with commercial inoculants of Glomus intraradices, a mixture of G. intraradices + Bacillus subtilis, a fertilization treatment with NPK (220-180-120) and control without inoculation. Completely randomized design with four replications on a factorial arrangement was used, with four inoculant treatments and five potato genotypes. The data of ten agronomic characters, that included the weight of the tubers and biomass per plant, were subjected to analysis of variance and then to tests of mean comparison of Scott-Knott and processed with Infostat software. Results: Inoculation treatments with mycorrhizae or mycorrhizae with rhizobacteria, statistically surpassed (P < 0.05) the controls in the case of weight of tubers per plant, the average weight of tubers, number of tubers per plant and diameter of the tuber, in addition to the height of the plant at 15 and days after the transplant; in the case of fresh foliage weight per plant and the degree of senescence of the foliage, the control treatments statistically surpassed (P < 0.05) to the inoculation treatments. For other characters, number of sprouts per plant, the number of leaves and number of flowers per plant, no significant differences (P > 0.05) was found. Conclusions: The use of microbial inoculants based on mycorrhizae and rhizobacteria, improves the productivity of potato seed tubers under greenhouse conditions.Keywords: Glomus intraradices, agronomic performance, in vitro seedlings, potato varieties, greenhouse

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Respon Pertumbuhan Bibit Kelapa Genjah Terhadap Berbagai Dosis Pupuk Organik [The Response of Dwarf Coconut Seedling Growth on the Different Dose of Organic Fertilizer]

Buletin Palma ◽

10.21082/bp.v19n1.2018.47-56 ◽

2018 ◽

Vol 19 (1) ◽

pp. 47

Author(s):

Alfred Pahala Manambangtua, SP ◽

Linda Trivana ◽

Yulianus Rompah Matana

Keyword(s):

Seedling Growth ◽

Negative Impact ◽

Organic Fertilizer ◽

Dry Weight ◽

P Elements ◽

Randomized Design ◽

Coir Dust ◽

Number Of Leaves ◽

Goat Manure ◽

The Cost

One of the way to reduce the negative impact of the use of inorganic fertilizer is the using of organic fertilizer. Organic fertilizer in compsted form of compos has an important role in the improvement of chemical, physical, and biological of the soil and as a source plants nutriens. The organic fertilizer used in this research comes from coconut coir dust and composting of goat manure. Dust husk contains nutrient elements such as N, P, K, Ca, Fe, Mg, Na, Mn, Cu, Zn and Al. Goat manure contains relatively high nutrients because the goat muck is mixed with urine which also contains nutrients. Coir dust is a waste of coconut fiber. This research aims to determine the response of seedling growth of coconut seedling to organic fertilization. The research was conducted at Greenhouse, Indonesian Palma Plant Research Institute, from September to March 2017. The research used Completely Randomized Design (RAL) with five treatments and six replications to obtain 30 experimental units. The treatment tested was the dosage of organic fertilizer seedlings, consisting of no organic fertilizer (control), 250 g organic fertilizer, 500 g organic fertilizer, organic fertilizer 750 g, organic fertilizer 1.000 g. Organic fertilizer gives a real effect on plant height, stem circumference, and the content of K elements in the plant tissue. However, organic fertilizer fertilization does not affect for the number of leaves, root dry weight, dry weight of stem, the content of N and P elements in plants significantly. The use of organic fertilizer on coconut seeds can reduce the cost of fertilization using inorganic fertilizers and improve soil structure. ABSTRAKSalah satu cara untuk mengurangi dampak negatif dari penggunaan pupuk anorganik adalah penggunaan pupuk organik. Pupuk organik dalam bentuk yang telah dikomposkan berperan penting dalam perbaikan sifat kimia, fisika, dan biologi tanah serta sebagai sumber nutrisi tanaman. Pupuk organik yang digunakan dalam penelitian ini berasal dari debu sabut kelapa dan pengomposan kotoran kambing. Debu sabut mengandung unsur hara seperti N, P, K, Ca, Mg, Fe, Na, Mn, Cu, Zn, dan Al. Kotoran kambing mengandung unsur hara yang relatif tinggi karena kotoran kambing tercampur urine yang juga mengandung nutrisi. Debu sabut merupakan limbah dari penyeratan sabut kelapa. Penelitian ini bertujuan untuk mengetahui respon pertumbuhan bibit kelapa genjah terhadap pemupukan organik. Penelitian dilaksanakan di Rumah Kaca, Balai Penelitian Tanaman Palma, Manado dari bulan September-Maret 2017. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan dan enam ulangan sehingga diperoleh 30 satuan percobaan. Perlakuan yang diuji adalah dosis pupuk organik perbibit, yang terdiri atas tanpa pemberian pupuk organik (kontrol), pupuk organik 250 g, pupuk organik 500 g, pupuk organik 750 g, pupuk organik 1.000 g. Pemberian pupuk organik memberi pengaruh nyata terhadap tinggi tanaman, lingkar batang, dan kandungan unsur K dalam jaringan tanaman. Namun pemupukan pupuk organik tidak mempengaruhi untuk jumlah daun, berat kering akar, berat kering batang, kandungan unsur N dan P pada tanaman secara nyata. Penggunaan pupuk organik pada bibit kelapa dapat mengurangi biaya pemupukan menggunakan pupuk anorganik dan memperbaiki struktur tanah.

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Mutation induction in the pineapple (Ananas comosus L. Merr) using colchicine

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/905/1/012082 ◽

2021 ◽

Vol 905 (1) ◽

pp. 012082

Author(s):

Rosmaina ◽

R Elfianis ◽

F Mursanto ◽

A Janna ◽

T Erawati ◽

...

Keyword(s):

Plant Height ◽

Leaf Shape ◽

Economic Value ◽

Colchicine Treatment ◽

Ananas Comosus ◽

Mutation Induction ◽

Tropical Fruit ◽

Randomized Design ◽

Number Of Leaves ◽

And Control

Abstract Pineapple is a tropical fruit that has high economic value. Mutation is a method to increase plant diversity which plays an essential role in plant improvement. This study aimed to induce mutations in pineapple using colchicine. This study was arranged in a factorial completely randomized design. The first factor was pineapple genotypes (i.e., Gemilang, Bangka, Queen, and Suska Kualu) and the second factor was colchicine concentration (i.e., 0.03%, 0.04%, 0.05%, and control). There were 16 treatments with five repetitions so the total was 80 experimental units. Observations were made for three months after treatment in the vegetative phase. Observation parameters included leaf shape, leaf color, plant height, number of leaves, leaf width, and length of stomata. This study indicated that the interaction between genotype and colchicine significantly differed in plant height and the number of leaves. Colchicine significantly increased the length of stomata, and genotype significantly affected all observed parameters. This study concluded that 0.05% colchicine significantly increased plant height (26.67%) and the number of leaves (48.98%) in the Gemilang genotype but decreased plant height and leaf number in other genotypes. This study suggests the need for observation of the flowering phase and fruit quality due to colchicine treatment.

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