scholarly journals PHARMACOGNOSTIC, PHYSICOCHEMICAL, AND PHYTOCHEMICAL STUDIES ON STEM BARK OF ZANTHOXYLUM ARMATUM DC.

2019 ◽  
pp. 470-474
Author(s):  
JOTHI G ◽  
KEERTHANA K ◽  
SRIDHARAN G
Keyword(s):  
Transverse Section ◽  
Microscopic Analysis ◽  
Fluorescence Analysis ◽  
Stem Bark ◽  
Ash Content ◽  
Water Soluble ◽  
Variable Size ◽  
Zanthoxylum Armatum ◽  
Additional Support ◽  
Phytochemical Studies

Objectives: To analyze the microscopic, macroscopic, physicochemical, and phytochemical standards of the Zanthoxylum armatum DC. (stem bark). Methods: Pharamacognostic studies, namely macroscopic, microscopic, and powder microscopic analysis of stem bark were carried out. Physicochemical standards - ash content including total ash content, water soluble, and acid insoluble ash values, moisture content by loss on drying, solubility and extractive values of Z. armatum DC. were determined. Preliminary phytochemical screening, fluorescence analysis and quantification of alkaloids, flavonoids, phenol, tannins, saponins, and terpenoids were also studied. Results: The macroscopic studies of stem bark showed the stem and its branches, armed with long, sharp prickles with variable size. The transverse section of bark showed the phellogen, phelloderm, cortex, phloem, and medullary rays that are characteristics to Z. armatum DC. The results of physicochemical standards give the identity and purity of the selected sample. Phytochemical studies revealed the presence of alkaloids, flavonoids, phenols, tannins, saponins, and terpenoids. Conclusion: The present findings provide the pharmacognostic, physicochemical, and phytochemical information about the stem bark of Z. armatum and this might be useful by providing additional support with regard to its identification and standardization parameters.

scholarly journals Pharmacognostical Standardization and Phytochemical Studies on the leaves of Solanum torvum Sw

2019 ◽  
Vol 9 (4-A) ◽  
pp. 290-295
Author(s):  
L Janarthanan ◽  
BR Balakrishnan ◽  
V Karthikeyan ◽  
P Senniappan ◽  
BS Venkateswarlu ◽  
...  
Keyword(s):  
Vascular System ◽  
Microscopic Analysis ◽  
Volatile Oil ◽  
Water Soluble ◽  
Flowering Plant ◽  
Solanum Torvum ◽  
Crude Fiber Content ◽  
Physico Chemical ◽  
Solanaceae Family ◽  
Phytochemical Studies

A genus of plant belongs to the family Solanaceae well distributed in India more than 26 species which are found naturalised in India. Solanum torvum Sw. is a medium sized flowering plant in the Solanaceae family that is found in India, Malaysia. Transverse section of lamina showed the adaxial part has thick, short hump; the midrib and the adaxial hump have thin, angular epidermal cells. The upper part of adaxial hump has a few layers sclerenchyma cells. The inner layer of the adaxial midrib also has few layers of thick walled cells. The ground tissue consists of wide circular thin walled parenchyma cells with narrow inter cellular spaces. The vascular system of the midrib showed bi-collateral structure. Non glandular, profusely branched, thick walled, lignified epidermal trichome occurs as both on the veins and lamina. Physico-chemical standards such as Foreign Matter, Total Ash, Water Soluble Ash, Sulphated Ash, Loss on Drying, Water Soluble Extractive, Alcohol Soluble Extractive and Crude Fiber Content in percentage were estimated. Preliminary phytochemical screening of appropriate solvent extracts showed the presence of Alkaloids, Amino Acids, Carbohydrates, Cellulose, Lignin, Fats & Fixed Oils, Flavonoids, Glycosides, Tannins, Proteins, Starch, Steroids and Triterpenoids and absence of Volatile Oil, Mucilage and Pectin. Microscopic analysis and other parameters were informative and provide valuable information in the identification, standardization of Solanum torvum leaves. Keywords: Solanum torvum, Solanaceae, leaf, Microscopical evaluation.

Standardization and Phytochemical screening of Carica papaya seeds

2021 ◽  
pp. 4540-4546
Author(s):  
Varsha Singh ◽  
Aleza Rizvi ◽  
Udaivir Singh Sara
Keyword(s):  
Physicochemical Parameters ◽  
Plant Secondary Metabolites ◽  
Chemical Properties ◽  
Fluorescence Analysis ◽  
Water Soluble ◽  
Correct Identification ◽  
Phytochemical Screening ◽  
Physico Chemical ◽  
Hexane Extracts ◽  
Phytochemical Studies

The present work focus to evaluate the physicochemical and preliminary phytochemical studies on the seeds of family Caricaceae. The plants resources which are used in pharmaceutical formulation standardization was carried out on the basis of organoleptic properties, physical characteristics, and physico-chemical properties. Different Physicochemical parameters ash values, extractive values, loss on drying, foreign matter, fluorescence analysis, and pH were evaluated. Macroscopical characteristics and Physicochemical parameters like total ash, acid insoluble ash and water soluble ash were found to be 83.7%, 71.7% and 61.5% w/w respectively. Hexane, ethyl acetate, ethanol and water soluble extractive values (hot)were 7.6%, 11.6%, 27.4%, 37.5%w/w respectively. The pH of 1% and 10% aqueous solution was found to be 3.57 and 3.78 respectively. Preliminary phytochemical screening showed the presence of Tannins, Proteins and amino acids, Glycosides, Phenolic compounds, Carbohydrates, Saponins, Alkaloids and Flavonoids. Thin layer chromatographic studies also had been done on ethanolic and hexane extracts. HPTLC fingerprinting is a valuable method for the quantitative determination of phytochemicals present in plant extract. These studies aim to investigating referential information for correct identification and standardization of this plant secondary metabolites.

scholarly journals Pharmacognostical and phytochemical studies on leaves of Oxalis corniculata Linn

2016 ◽  
Vol 5 (6) ◽  
pp. 225-229
Author(s):  
Shalini Karunanithi ◽  
◽  
Vijaya Bharathi Rajkishore ◽  
Vandhana. Ganesh Pol ◽  
Sitharathul Mumthana. M Mumthana. M ◽  
...  
Keyword(s):  
Research Work ◽  
Fluorescence Analysis ◽  
Water Soluble ◽  
Physiochemical Parameters ◽  
Soxhlet Apparatus ◽  
The Family ◽  
Annual Herb ◽  
Cardiac Disorders ◽  
Phytochemical Studies

Oxalis corniculata Linn is an annual herb commonly known as Indian horrel belonging to the family Oxalidaceae. It is found to uses as, anthelminitic, astringent, diuretic, emmenagogue, febrifuge, cardiac disorders, diarrheoa, dysmenorrhoea, hepatic disorders, eye disease, scurvy and toxicity. This research work deals with the Pharmacognostical and Phytochemical studies on leaves of Oxalis corniculata Linn microscopic photographs of transverse section (T.S) of Oxalis corniculata should distinct presentation of epidermal trichomes, calcium oxalate crystals and stomata. Standardization of the plant were done with the help of extractive values [ alcohol soluble extractive (16%w/w), water soluble extractive (24%w/w)], total ash (15%w/w), acid insoluble ash (1.7%w/w), water soluble ash (1.8%w/w), sulphated ash (0.9%w/w). Pytochemical analysis showed the presence of carbohydrates, glycosides, flavanoids, phenols, tannins, volatile oils, aminoacids and proteins. The dried coarsely powdered Oxalis corniculata plant was extracted with ethanol 600C in soxhlet apparatus for 24 hours. Another extract is prepared in cold maceration method for 7 days using water. The both extract was collected and preserved in a vaccum desicator. Fluorescence analysis was carried out for the plant powder and extract. This study various Pharmacognostical and physiochemical parameters for the identification and standardization of the plant material and Phytochemical screening for determination of active constituents.

scholarly journals Pharmacognostic studies of the stem bark of Chloroxylon swietenia DC

2015 ◽  
Vol 3 (04) ◽  
pp. 01-05 ◽  
Author(s):  
Nilip Kanti Deb ◽  
Anoop Singh ◽  
Devendra Singh Rathore ◽  
Gouri Kumar Dash ◽  
Jhuma Deb
Keyword(s):  
Plant Material ◽  
Transverse Section ◽  
Scientific Information ◽  
Research Work ◽  
Fluorescence Analysis ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Powdered Sample ◽  
Phytochemical Screening ◽  
Chloroxylon Swietenia

Chloroxylon swietenia DC. (Family: Rutaceae) is an important traditional medicinal plant used in the treatment of various ailments like fungal infection of skin, rheumatism, common cold, cough, ophthalmic infection and cataract, wounds and as an astringent. However, detailed scientific information is not available to identify the plant material, in order to ascertain its quality and purity. In this paper, we report the pharmacognostic evaluation of the bark for the purpose of its identification and differentiation from related species. The macroscopy, microscopy, physicochemical parameters such as moisture content, ash values, extractive values, fluorescence analysis and preliminary phytochemical analysis of the bark were investigated. Transverse section of the bark shows presence of cork, cortex, medullary rays, and stone cells. Presence of cork, stone cells, phloem fibres and parenchyma cells which were also observed in the powdered sample of the bark. The result of preliminary phytochemical screening indicated presence of alkaloids, steroids, carbohydrates, proteins, phenolic compounds, tannins flavonoids and triterpenoids. The present study will be useful for its identification prior to carrying out further research work. The findings of this study will facilitate pharmacognostic standardization of the plant material and aid in the preparation of a herbal monograph for the species.

scholarly journals KARAKTERISASI SIMPLISIA DAN EKSTRAK KULIT BATANG SEKILANG (Embelia borneensis Scheff.)

2021 ◽  
Vol 6 (2) ◽  
pp. 196-205
Author(s):  
Risa Supriningrum ◽  
Reksi Sundu ◽  
Triswanto Sentat ◽  
Rakhmadhan Niah ◽  
Eka Kumalasari ◽  
...  
Keyword(s):  
Stem Bark ◽  
Ash Content ◽  
Water Soluble ◽  
Bark Extract ◽  
Average Moisture Content ◽  
Soluble Extract ◽  
Content Determination ◽  
Extract Content ◽  
The Mean

The Sekilang plant (Embelia borneensis Scheff.) Is one of the plants used by the Dayak community for fishing, leech repellent and as an ingredient for hair care. Empirical use of plants can be developed into products based on natural ingredients. This must be supported by research data. As a first step, the simplicia and ethanol extract of the stem bark were characterized. The research objective was to determine the specific and non-specific characteristics of the simplicia and the extracts of the bark of the sekilang. The research stage includes plant determination, collection of raw materials, manufacture of simplicia, extract preparation, organoleptic, microscopic testing, phytochemical screening, determination of water soluble extract content, determination of ethanol soluble extract content, determination of water content and determination of ash content. Microscopy test results of sekilang stem bark showed the presence of fiber fragments, stone cells, and oxalate crystals. The average water soluble content for simplicia and sekilang bark extract was 7.5% and 80%. The average ethanol soluble content for simplicia and extract was 6% and 30%. The average moisture content for the simplicia and extract was 9.5% and 12%. The mean total ash content for simplicia and extract was 5% and 8.5%. The mean acid insoluble ash content for simplicia and extract was 1% and 0.5%.

Pharmacognostic studies of Morinda brevipes S. Y. Hu

2019 ◽  
Vol 48 (3) ◽  
pp. 401-407
Author(s):  
Rong Li ◽  
Xiaojing Lin ◽  
Genqiu Tang ◽  
Junni Li ◽  
Dong Wang ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Transverse Section ◽  
Volatile Oils ◽  
Needle Crystal ◽  
Cork Cell ◽  
Registration Number ◽  
Phytochemical Studies ◽  
First Time ◽  
Layer Chromatography ◽  
Inequality Type

DNA barcoding of ITS and psbA-trnH regions, histochemistry as well as thin layer chromatography (TLC) of Morinda brevipes S.Y. Hu were analyzed. Transverse section of root revealed the presence of cortex, xylem, cork cell, stone cells, and calcium oxalate sandy crystal. The lower epidermis cells showed many stoma in paracytic or inequality type. Spiral vessel and tiny calcium oxalate needle crystal usually appeared in the powder. TLC showed the presence of emodin in M. brevipes. Phytochemical studies revealed the existence of carbohydrates, saponins, tannins, flavones, anthraquinones, alkaloids and volatile oils. The ITS and psbAtrnH sequences were found for the first time which were submitted to NCBI to obtain the GenBank registration number. This study might play an important role in the identification, and utilization of M. brevipes for various purposes.

Standardization and bacteria inhibitory test of purified extract of mahogany (Swietenia mahagoni (L.) Jacq) seeds and leaves

2019 ◽  
Vol 10 (3) ◽  
pp. 2132-2138
Author(s):  
Virsa Handayani ◽  
Rezki Amriati Syarif ◽  
Ahmad Najib ◽  
Aktsar Roskiana Ahmad ◽  
Abdullah Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heavy Metal ◽  
Bitter Taste ◽  
Drying Shrinkage ◽  
Ash Content ◽  
Water Soluble ◽  
Lead Contamination ◽  
Specific Parameter ◽  
Chemical Content ◽  
Swietenia Mahagoni

Mahogany (Swietenia mahagoni (L.) Jacq) is one of the plants that is often used by the community as traditional medicine. One of them is antifungal, antibacterial, antidiabetic, and eczema. This study aims to obtain standardized extracts from mahogany seeds and leaves. Standardization of purified extract of mahogany has been carried out according to the monographs of extract standardization guidelines, which include testing of specific and non-specific parameters. The results of the specific parameter testing showed that the purified extract of mahogany seeds is a thick extract, brown to reddish, smells distinctive and has a bitter taste. While the purified extract of mahogany leaves is a thick extract, greenish-brown in color, distinctive smell and has a bitter taste. The chemical content of purified extract of mahogany seeds and leaves showed the presence of flavonoids, alkaloids, terpenoids and saponins. Water-soluble essence levels in mahogany seeds and leaves was 14.84% and 10.28%. While the ethanol-soluble essence levels in mahogany seeds and leaves were 15.38% and 12.43%. Testing of non-specific parameters on mahogany seeds and leaves showed the results of drying shrinkage levels of 0.22% and 8.84%, moisture content of 2.60% and 4.04%, total ash content of 1.71% and 1.93%, levels acidic insoluble ash 0.38% and 0.32%, Total Plate Number (ALT) of mahogany seed bacteria 1x102 colonies/g, Number of mahogany mold seeds 4x10 colonies/g, heavy metal lead contamination and cadmium in mahogany seeds 0.0607µg/g and<0.003µg/g. The inhibitory diameter of each concentration of seeds against Escherichia coli, 3%, 5%, 7%, and 9%, is 12,67; 13,67; 17,67; and 19,67 mm, respectively. The inhibitory diameter of each concentration of leaves against Escherichia coli, 3%, 5%, 7%, and 9%, is 10,27; 10,90; 13,46; and 15,68 mm, respectively.

scholarly journals Standardization of Mango (Mangifera Indica L.) Peel Simplisia of Gadung Variety

2021 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Andini Andini ◽  
Cindy Fernanda Putri
Keyword(s):  
Moisture Content ◽  
Mangifera Indica ◽  
Quality Standard ◽  
Ash Content ◽  
Water Soluble ◽  
Raw Material ◽  
Yellow Colour ◽  
Water Soluble Compound ◽  
Mango Peel ◽  
Compound Concentration

Mango peel (Mangfera indica L.) has many pharmacological effects as a traditional medicine. Therefore, standardization of mango peel simplisia needs to be done as a preparation of phytopharmaca raw material. This research aimed to obtain standardization of mango peel simplisia include specific and non-specific parameter. The research procedures include plant determination, simplisia preparation as well as specific standardization test (includes organoleptic, water-soluble compound concentration, and ethanol solution compound concentration) and nonspecific standardization test (includes moisture content, dried shrinkage, total ash content and acid insoluble ash content). The specific organoleptic parameters of dried mango peel simplisia have a distinctive sweet aroma, bitter taste, and brownish yellow colour. Water-soluble and ethanol-soluble concentrations are 22,36% ± 1,17% and 9,56% ± 0,07%. Moisture content is 9,09% ± 1,44%. Dried shrinkage rate is 0,19% ± 0,04%. Total ash and acid insoluble ash contents are 4,11% ± 0,10% and 0,14% ± 0,03%. The mango peel simplisia has met the quality standard of the raw material.

scholarly journals Pharmacognostic Evaluation of Curcuma longa L. Rhizome and Standardization of its Formulation by HPLC Using Curcumin as Marker

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Abraham A ◽  
Samuel S ◽  
Mathew L
Keyword(s):  
Cross Section ◽  
Curcuma Longa ◽  
Microscopic Analysis ◽  
Fluorescence Analysis ◽  
Phytochemical Analysis ◽  
Diagnostic Features ◽  
Starch Grains ◽  
Qualitative And Quantitative ◽  
Polyherbal Formulation ◽  
Laboratory Procedures

Objective: To evaluate Curcuma longa rhizome by pharmacognostic and phytochemical analysis and standardize one of its formulations by HPLC. The present work includes macroscopic study, microscopic analysis of cross section and powder of rhizome, fluorescence analysis, qualitative and quantitative phytochemical assay and chromatographic fingerprinting of its polyherbal formulation, Pathyashadangam kwath using curcumin as marker carried out as per standard laboratory procedures. The study helps to provide both diagnostic features for identification and preventing adulteration of Curcuma longa L and HPLC chromatogram for standardisation of its formulation. Results: Microscopic analysis revealed the presence of a broad parenchymatous cortex with abundant starch grains in the cross section, spiral vessels, starch grains having hilum towards the narrower end, fibres and cells with oleo resin in powder microscopy. HPLC analysis revealed the presence of three peaks corresponding to curcuminoids in the formulation. Conclusion: Pharmacognostic and phytochemical evaluation can be used for confirming the identity of Curcuma longa rhizome and HPLC using curcumin as marker can be used for standardisation of its formulations.

scholarly journals Oil-based mud waste reclamation and utilisation in low-density polyethylene composites

2020 ◽  
Vol 38 (12) ◽  
pp. 1331-1344
Author(s):  
Shohel Siddique ◽  
Kyari Yates ◽  
Kerr Matthews ◽  
Laszlo J Csetenyi ◽  
James Njuguna
Keyword(s):  
Inductively Coupled Plasma ◽  
Oil And Gas ◽  
Microscopic Analysis ◽  
Optical Emission ◽  
Fluorescence Analysis ◽  
Xrd Analysis ◽  
Low Density Polyethylene ◽  
Emission Spectrometry ◽  
Low Density ◽  
X Ray

Oil-based mud (OBM) waste from the oil and gas exploration industry can be valorised to tailor-made reclaimed clay-reinforced low-density polyethylene (LDPE) nanocomposites. This study aims to fill the information gap in the literature and to provide opportunities to explore the effective recovery and recycling techniques of the resources present in the OBM waste stream. Elemental analysis using inductively coupled plasma–optical emission spectrometry (ICP-OES) and X-ray fluorescence analysis, chemical structural analysis by Fourier transform infrared (FTIR) spectroscopy, and morphological analysis of LDPE/organo-modified montmorillonite (LDPE/MMT) and LDPE/OBM slurry nanocomposites by scanning electron microscopy (SEM) have been conducted. Further analysis including calorimetry, thermogravimetry, spectroscopy, microscopy, energy dispersive X-ray analysis and X-ray diffraction (XRD) was carried out to evaluate the thermo-chemical characteristics of OBM waste and OBM clay-reinforced LDPE nanocomposites, confirming the presence of different clay minerals including inorganic salts in OBM slurry powder. The microscopic analysis revealed that the distance between polymer matrix and OBM slurry filler is less than that of MMT, which suggests better interfacial adhesion of OBM slurry compared with the adhesion between MMT and LDPE matrix. This was also confirmed by XRD analysis, which showed the superior delamination structure OBM slurry compared with the structure of MMT. There is a trend noticeable for both of these fillers that the nanocomposites with higher percentage filler contents (7.5 and 10.0 wt% in this case) were indicated to act as a thermal conductive material. The heat capacity values of nanocomposites decreased about 33% in LDPE with 7.5 wt% MMT and about 17% in LDPE with 10.0 wt% OBM slurry. It was also noted, for both nanocomposites, that the residue remaining after 1000°C increases with the incremental wt% of fillers in the nanocomposites. There is a big difference in residue amount (in %) left after thermogravimetric analysis in the two nanocomposites, indicating that OBM slurry may have significant influence in decomposing LDPE matrix; this might be an interesting area to explore in the future. The results provide insight and opportunity to manufacture waste-derived renewable nanocomposites with enhanced structural and thermal properties.

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