scholarly journals Evaluation of Antidiabetic Activity of Sargassum tenerrimum in Streptozotocin-Induced Diabetic Mice

2021 ◽  
Author(s):  
A. Philomena Joy Lindsey ◽  
Reya Issac ◽  
M. Lakshmi Prabha ◽  
R. Emilin Renitta ◽  
Angeline Catherine ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Methanolic Extract ◽  
Serum Triglyceride ◽  
Hdl Cholesterol ◽  
Developed Countries ◽  
Antidiabetic Activity ◽  
Diabetic Mice ◽  
Sedentary Life Style ◽  
Sargassum Tenerrimum

Diabetes mellitus has become the most predominant disease in most of the developing and developed countries. Diabetes could occur at any stage of life for which a change in sedentary life style is the possible solution. Despite the introduction of several medications, to curtail the side effects associated with diabetes mellitus, there is no medication or treatment for complete cure. Existing medications and treatments are targeted to mitigate the intensity of the disease which abruptly could result in organ failure. Rather than the available types of treatments, natural medication is reported to produce commendable results. Extracts of many medicinal plants and seaweeds have been documented to possess anti-diabetic properties. A brown alga, S. tenerrimum, found on the west coast of India, is chosen in this study to analyse the anti-diabetic property of its methanolic extract. This extract was used to treat streptozotocin-induced diabetic mice. An experimental design was framed based on acute toxicity studies to determine the change in blood glucose level, body weight, lipid profile, liver enzymes (SGPT and SGOT) and the renal function markers (urea and creatinine). After 15 days of treatment, an increase in body weight and HDL cholesterol was observed while the total cholesterol, VLDL, LDL, serum triglyceride, SGOT, SGPT, creatinine and urea decreased with 250 mg/Kg.bw of S. tenerrimum methanolic extract in Streptozotocin-induced diabetic mice group. This difference is significant (p<0.05) when compared with a healthy non-diabetic mice group and a treated diabetic mice group. This proves that the methanolic extract of S. tenerrimum possess anti-diabetic property. Further research could explore the active compounds that can be used in the formulation of herbal medicine for diabetes mellitus.

Antidiabetic activity of aqueous extract of leaves of cnestis ferruginea vahl ex DC. (Connaraceae) on alloxan-diabetic induced mice.

2021 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Emile K. Begbin ◽  
Edwige A. Odoh ◽  
Ernest N. Zougrou ◽  
Landry Claude A. Kablan ◽  
Diéneba Kone-Bamba ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Aqueous Extract ◽  
White Blood Cells ◽  
Hdl Cholesterol ◽  
Treated Group ◽  
Antidiabetic Activity ◽  
Metformin Group ◽  
Hematological And Biochemical Parameters ◽  
Group B

Abstract Background: Cnestis ferruginea is a plant species widely used in the traditional african medicine to treat diabetes mellitus. The present study aimed to evaluate the antidiabetic activities of the aqueous extract of the leaves of Cnestis ferruginea (AECF) in diabetic mice. Methods: The mice were grouped into five groups of five animals per group: Group A was not induced with alloxan, Group B animals were induced but not treated, Group C animals were treated with 500 mg / kg of BW of metformin, Group D and E animals were treated with 100, 200 mg/kg BW of AECF respectively. The extracts were administered to the animals orally for 14 days. Fasting blood sugar was measured by a glucometer. Serum concentrations of hematological and biochemical parameters were measured by standard methods. Results: The animals administered with 100 and 200 mg/Kg B.W of extract showed highly significant decrease (P ˂ 0.0001) in blood sugar level compared to the untreated animals. The 100 mg / kg BW dose of AECF produce low significant decrease (P <0.05) of total cholesterol, HDL Cholesterol, AST, ALT, urea, levels of white blood cells, platelets, hematocrit. The 200 mg / kg BW dose of AECF produce in addition significant decrease (P <0.01) of AST and urea. Conclusion: The 200 mg / kg dose of BW of AECF had a greater antidiabetic activity than the dose of 100 mg / kg BW. Keywords: Cnestis ferruginea; antidiabetic activity; alloxan; diabetes mellitus.

Effect of Crude Leaves Extract of Bersama Abyssinica on Blood Glucose Level and Serum Lipid Level of Streptozotocin-Induced Diabetic Mice: Evidence for In vivo Antidiabetic Activity

2021 ◽  
Vol 19 ◽  
Author(s):  
Zemene Demelash Kifle ◽  
Agumas Alemu Alehegn ◽  
Baye Yrga Adugna ◽  
Abebe Basazn Mekuria ◽  
Engidaw Fentahun Enyew
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Serum Lipid ◽  
Lipid Level ◽  
Serum Lipid Level ◽  
Diabetic Mice ◽  
Lipid Levels ◽  
Antidiabetic Effect ◽  
Bersama Abyssinica

Background: Diabetes mellitus is one of the major and common metabolic, and chronic disorders in the world. Several medicinal plants have been used globally for the management of diabetes mellitus. The current study aimed to study the anti-hyperglycemic and anti-hyperlipidemic effects of Bersama abyssinica. Methods: Antidiabetic effect of 80% methanolic crude extract of Bersama abyssinica was studied in repeated dose-treated STZ-induced diabetic mice model. The activities of Bersama abyssinica on serum lipid level and body weight were investigated on STZ-induced diabetic mice. Data were analyzed using one-way ANOVA and significant when the p-value was less than 0.05. Results: All doses of the crude 80% methanolic extract of Bersama abyssinica (100 mg/kg, 200 mg/kg, and 400 mg/kg) exhibited a noticeable BGL reduction when compared with baseline blood glucose level and diabetic control on the 7th and 14th days of administration. Moreover, the higher dose of the extract (at 400 mg/kg) significantly (p < 0.001, 54.3%) decreased the BGL in STZ-induced diabetic mice. The maximum decrement in fasting BGL was achieved at the 14th days: 34.92%, 41.10%, 54.30%, and 59.66%, respectively for BAC 100 mg/kg, BAC 200 mg/kg, BAC 400 mg/kg, and GLC 5 mg/kg treated groups. Bersama abyssinica also displayed a significant (p < 0.05) improvement of serum lipid levels and body weight. Conclusion: Bersama abyssinica crude extract exhibited a significant antidiabetic effect and prevented body weight loss in streptozotocin-induced diabetic mice. The finding also confirmed the valuable biochemical activity of Bersama abyssinica by improving serum lipid levels.

scholarly journals Evaluation of potential antidiabetic activity of abelmoschus esculentus and evidence of possible pharmacokinetic interaction with metformin

2022 ◽  
Vol 8 (4) ◽  
pp. 263-266
Author(s):  
Raja Chakraverty ◽  
Debalina Sardar ◽  
Pranabesh Chakraborty
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Animal Models ◽  
Pharmacokinetic Interaction ◽  
Diabetic Control ◽  
Abelmoschus Esculentus ◽  
Antidiabetic Activity ◽  
Plant Materials ◽  
Glucose Levels ◽  
Test Study

The study is aimed at the evaluation of potential activity of and possible interaction with metformin in animal Models of Diabetes Mellitus. Study objectives include study the anti-diabetic effect of for Diabetes Mellitus in animal models and also to study the effect of Abelmoschus esculentus with metformin and explore any interaction. Plant material was collected () followed by extraction of plant materials () Exudate collection of and activity test study was done (acute toxicity study, according to standard OECD guidelines) Experimental animals were divided into groups. Dosing was done for 28 days. Biochemical parameters were studied. Histopathology studies are done. Results showed that in this study administrations of Abelmoschus esculentus extract (2000mg/kg body weight) Metformin with extract (5mg/kg b.w. and 2000mg/kg body weight and Metformin 5mg/kg body weight decreased elevated blood glucose levels significantly from first to fourth week compared to diabetic control rats and showed minimal safety concerns.

scholarly journals ANTIDIABETIC ACTIVITY OF KALANCHOE PINNATA IN ALLOXAN-INDUCED DIABETIC RATS

2019 ◽  
pp. 241-245
Author(s):  
LATHA OPHELIA GEORGE ◽  
RADHA HR ◽  
SOMASHEKARAIAH BV
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Methanolic Extract ◽  
Public Health Problem ◽  
Diabetic Rats ◽  
The Body ◽  
Antidiabetic Activity ◽  
Percentage Increase ◽  
Group I ◽  
Kalanchoe Pinnata

Objective: The main purpose of the present study was to analyze the antidiabetic activity of methanolic extract of the leaf of Kalanchoe pinnata in alloxan-induced diabetic rats. Methods: Diabetes mellitus (DM) is a chronic metabolic and endocrine disease regarded as a serious global public health problem. Albino Wistar rats were divided into four groups. Group I (normal) received drinking water throughout the course till 20 days. Groups II-IV received alloxan (120 mg/kg b.w) i.p on the 1st day of the study period. Group III animals received glibenclamide (10 mg/kg p.o) and Group IV received K. pinnata (200 mg/kg b.w p.o) for 20 days. The body weight, blood glucose level, serum urea, blood urea nitrogen (BUN), creatinine, cholesterol, total protein (TP), uric acid, and tissue (liver) antioxidant parameters: Malondialdehyde (MDA) and glutathione (GSH) were measured. Results: K. pinnata treated rats showed the percentage increase in the body weight, decrease in the blood sugar level, creatinine, TP level, urea, uric acid, and BUN, MDA, and GSH when compared to alloxan-induced diabetic mellitus control rats. Thus, K. pinnata could be possibly employed to treat DM. Conclusion: This preliminary analysis tested the most promising profile. It seems that methanolic extract of the leaf of K. pinnata improved general, blood serum, and liver antidiabetic agent. However, further studies confirming its potential is certainly warranted.

scholarly journals Study of Blood Glucose and SGPT lowering effect of Eucalyptus Lanceolata Linn. in Induced Diabetic Rabbits

2015 ◽  
pp. 152-158
Author(s):  
MINGYUE YAOA
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
The Body ◽  
Antidiabetic Activity ◽  
Medicinal Uses ◽  
Diabetic Rabbit ◽  
Pharmaceutical Industries ◽  
Diabetic Rabbits ◽  
Plants Extract

The part of the plant to curing the diabetes mellitus and improves available data on plants with hypoglycemic effects. Antidiuretic potential of eucalyptus extracts has been evaluated in alloxan-induced diabetic rabbit. Here we used, plants extract about 256 kg were mix with 1000 ml methanol and keep in Flask. After filtering the extracts were exposed to rotary evaporator machine that methanol separated from plant extract. In the Nine rabbit were divided into three different groups. The body weight was recorded on every 6 days interval. The data obtained revealed that eucalyptus extracts reduced the glucose level effectively. Such treatment with extracts decreased the SGPT level. Therefore, it is concluded that the eucalyptus possesses significant antidiabetic activity. And finally, we recommend this plant for medicinal uses and for pharmaceutical industries to isolate pure drugs.

scholarly journals Antidiabetic Effects of the Ethanolic Root Extract ofUvaria chamaeP. Beauv (Annonaceae) in Alloxan-Induced Diabetic Rats: A Potential Alternative Treatment for Diabetes Mellitus

2018 ◽  
Vol 2018 ◽  
pp. 1-13
Author(s):  
Jonathan Emeka Emordi ◽  
Esther Oluwatoyin Agbaje ◽  
Ibrahim Adekunle Oreagba ◽  
Osede Ignis Iribhogbe
Keyword(s):  
Diabetes Mellitus ◽  
Alternative Treatment ◽  
Pancreatic Beta Cells ◽  
Hematological Parameters ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Root Extract ◽  
Acacia Senegal ◽  
Cholesterol Levels

Diabetes mellitus has been a menace to mankind from time immemorial. However, a natural product such asU. chamaeP. Beauv (Annonaceae) offers alternative treatment for diabetes mellitus. The study aimed at evaluating antidiabetic activity of the ethanolic root extract ofU. chamaein alloxan-induced diabetic rats. Diabetes was induced in Sprague Dawley rats after overnight fast with 150 mg/kg alloxan intraperitoneally. After 72 h, those with plasma glucose levels >200 mg/dl were classified as diabetic. Five diabetic rats in each group were treated daily for 14 days orally with 100, 250, and 400 mg/kg of the extract, glibenclamide (71 µg/kg) and pioglitazone (429 µg/kg), respectively, while another group was untreated. Control received 0.5 ml ofAcacia senegal. Effects of extract on glucose, other biochemical, and hematological parameters were evaluated.α-amylase andα-glucosidase inhibitory activities of extract and its fractions were also evaluated. Percentage inhibition and IC50values were determined. Diabetic control was achieved on the 7th day of the study with 100, 250, and 400 mg/kg of the extract showing glucose reduction of 72.14%, 78.75%, and 87.71%, respectively. The HDL-cholesterol levels of diabetic rats treated with extracts were significantly increased. Extract and its fractions causedα-amylase andα-glucosidase inhibition. Histologically, pancreas of diabetic rats treated with extract showed regenerated islet cells which were not seen in rats treated with glibenclamide and pioglitazone. This study showed thatU. chamaehas antidiabetic activity which may be throughα-amylase andα-glucosidase inhibition and regeneration of pancreatic beta cells. Also, it may reduce the risk of cardiovascular disease by increasing HDL-cholesterol levels.

scholarly journals Effect of 5-HTP and L-Dopa on lipid profile of Streptozotocin induced diabetic mice

2014 ◽  
Vol 15 (3) ◽  
pp. 149-155
Author(s):  
Ulka Yadav ◽  
Rajkumari Batham
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Serum Protein ◽  
Body Weight Gain ◽  
Glycosylated Hemoglobin ◽  
Hdl Cholesterol ◽  
The Body ◽  
Diabetic Mice

The present study was designed to test whether the precursor of serotonin (i.e.5-HTP) and dopamine (i.e. L-DOPA) can influence Body weight and certain hematological constitutes (i.e. blood glucose, glycosylated hemoglobin and serum protein) in streptozotocin induced diabetic mice. In the study, animals were divided into 6 groups. After 10 days at the termination of experiment body weight and blood glucose taken. Blood was collected from left ventricle and deposited to pathology for haematological analysis (i.e. blood Glucose, glycosylated heamoglobin, serum protein, triglyceride total cholesterol, HDL-cholesterol, LDC-cholesterol, and VLDL-cholesterol). Statistical analysis were done with the help of student’s ‘t’ test followed by analysis of variance (ANOVA). The result of the present study suggest that peripheral administration of 5-HTP (a precursor of serotonin) and L-DOPA (a precursor of dopamine) influences the body weight gain and lipid profile (i.e. triglyceride, total cholesterol, HDL-cholesterol , LDL-Cholesterol, VLDC-cholesterol) in streptozotocin induce diabetic mice. The finding of the present study supports the concept that insulin alone does not fully control diabetes. Other chemical substance like neurotransmitter/hormones may involve in controlling the diabetes.

scholarly journals Comparative efficacy of two medicinal plants and Amaryl® tablet (Glimepiride) in induced diabetes mellitus in rat

1970 ◽  
Vol 6 (2) ◽  
pp. 297-300
Author(s):  
PC Das ◽  
M Mostofa ◽  
AK Sarkar ◽  
M Ali
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
The Body ◽  
Antidiabetic Activity ◽  
Herbal Extracts ◽  
Comparative Efficacy ◽  
Trigonella Foenum Graecum ◽  
Coccinia Indica

Comparative efficacy of two indigenous herbal extracts, (Trigonella foenum-graecum seed & Coccinia indica leaves) with the commonly used drug Glimepiride were studied. Healthy young rats (n=30) of both sexes weighting between 150 to 200 gm were selected and divided into six equal groups, as A (Control), B (diabetic), experimental diabetic groups were-C, D, E and F, treated with combined extract of Methi seed and Telakucha leaves- @ 250mg, 500mg, 750mg and Glimepiride 800μg/kg body weight, respectively. The substances were given orally once daily for14 days. The blood glucose concentration were decreased significantly (P<0.01) in groups C, D, E, and F. The body weight increased significantly (P<0.05) by 2.3%, 3.9%, 4.8% and 10.4%, respectively. The herbal extracts significantly reduced in blood glucose level with the increased in body weight. It is indicated that antidiabetic activity of Trigonella foenum-graecum and Coccinia indica are evident that are traditionally used as the herbal preparations by the Ayurvedic practitioners for the control of diabetes mellitus. Keywords: Trigonella foenum graecum; Coccinia indica; Amaryl® Tablet (Glimepiride); Streptozotocin and antidiabetic effect; Diabetes mellitus; Rat DOI: 10.3329/jbau.v6i2.4825 J. Bangladesh Agril. Univ. 6(2): 297-300, 2008

scholarly journals Phytochemical Screening and Antidiabetic, Antihyperlipidemic, and Antioxidant Effects of Leptopus Cordifolius Decne. In Diabetic Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Shahid Rahman ◽  
Gul Jan ◽  
Farzana Gul Jan ◽  
Hafeez Ur Rahim
Keyword(s):  
Blood Glucose ◽  
Total Cholesterol ◽  
Group Treatment ◽  
Methanolic Extract ◽  
Antidiabetic Activity ◽  
Diabetic Mice ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Haemolytic Assay

Plants are well known in traditional herbal medicines for their hypoglycemic and hypolipidemic activities and are often used due to their accessibility, affordability, and corollary effects. Leptopus cordifolius has been reported to control diabetes in folkloric medicine, but no known scientific research has been conducted to assess the plausibility of this assertion. Therefore, the current study is aimed to investigate the antidiabetic and hypolipidemic effects of Leptopus cordifolius leaves in alloxan-induced diabetic mice. The antidiabetic and antihyperlipidemic evaluation was conducted in Swiss albino mice at doses of 150–250°mg/kg for 15°days. The blood glucose, total cholesterol, triglyceride, LDL, HDL, creatinine, ALP, SGPT, and SGOT levels were estimated according to standard procedures. Phytochemicals of leaves were analyzed using GC-MS analysis. Enzymatic antioxidant activity of the plant was investigated spectrophotometrically by carrying out superoxide dismutase, peroxidase, and catalase assays. The membrane stabilization potential of L. cordifolius leaf extracts was carried out using an in vitro haemolytic assay. The results revealed a dose response effect with the methanolic extract of L. cordifolius which had significant antihyperglycemic effects at 150–250°mg/kg in alloxan treated mice, although less than the positive control (glibenclamide). Hyperlipidemic activity was significant at 250 mg/kg. The biochemical parameters, such as total cholesterol, triglyceride, LDL, HDL, creatinine, ALP, SGPT, and SGOT, were significantly improved (p &lt; 0.01) by the methanolic extract of 250 mg/kg compared to the diabetic group. Treatment for 15 days showed significant elevation (p &lt; 0.01) of antioxidant enzymes. GC-MS analysis provided tentative identifications of 52 compounds in the methanolic extract of L. cordifolius, of which 12 compounds have reported antidiabetic activity. In conclusion, methanolic extract of L. cordifolius of 150 and 250°mg/kg body weight showed significant antidiabetic and antihyperlipidemic activities in alloxan-induced diabetic mice and, with further work, has the potential to be used to manage blood glucose and cholesterol levels.

scholarly journals In Vivo and In Vitro Antidiabetic Activity of Hydroalcoholic Extract of Dactylorhiza Hatagirea Roots: An Evaluation of Possible Phytoconstituents

2019 ◽  
Vol 9 (6-s) ◽  
pp. 76-81
Author(s):  
Rashmi Choukarya ◽  
Ashish Choursia ◽  
Jagdish Rathi
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Total Protein ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Root Extract ◽  
Test Protocol ◽  
Hydroalcoholic Extract

Diabetes mellitus is one of the most common endocrine disorder which causes microvascular and macrovascular complications. Several synthetic drugs used to treat diabetes have adverse effects on prolonged usage. This has given the impetus to the search for alternative medicines with no or less side effects. The plants of Orchidaceae family have displayed antimicrobial, anti-inflammatory, antioxidant, anticancer and antidiabetic activity. However, their antidiabetic properties are yet to be explored. This study sought to evaluate the antidiabetic potentials of hydroalcoholic extract of root of Dactylorhiza hatagirea (D. hatagirea) in diabetic rats. Qualitative analysis of various phytochemical constituents and quantitative analysis of total flavonoids were determined by the well-known test protocol available in the literature and quercetin was detected in hydroalcoholic extract of root of D. hatagirea under study by using RP-HPLC analysis. Percentage inhibition of α-amylase activity by D. hatagirea root extract was estimated with acarbose as the positive control, D. hatagirea extract show dose dependent activity. IC50 value of acarbose and extract was found to 35.33 and 224.45 µg/ml respectively. Diabetes was induced in wistar albino rats by administration of alloxan monohydrate (120 mg/kg; i.p.). The of hydroalcoholic extract of D. hatagirea at a dose of 100 and 200 mg/kg body weight was administrated at a single dose per day to diabetes-induced rats for a period of 15 day. The effect of hydroalcoholic extract of D. hatagirea root on blood glucose, total cholesterol (TR), triglycerides (TG), total protein and body weight were measured in the diabetic rats. The effect of hydroalcoholic extract of D. hatagirea root elicited significant reduction in blood glucose (p<0.001), (p<0.01), lipid parameters (TC, TG, total protein) (p<0.01) and significantly increased body weight at the dose of 200 mg/kg when compared with the diabetic-induced control. For future studies, phytochemcials responsible for various activities can be isolated and modified for pharmacological purpose.This study, therefore, justifies the use of the plant in the treatment of diabetes mellitus. Keywords: Dactylorhiza hatagirea, Phytochemical profile, Quercetin, 

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