scholarly journals Phytochemical analysis of different extracts of Pinda concanensis and Heracelum grande

2021 ◽  
Vol 11 (6-S) ◽  
pp. 100-103
Author(s):  
Sucheta Bidve ◽  
Sanjay Auti
Keyword(s):  
Secondary Metabolites ◽  
Biologically Active ◽  
Phytochemical Analysis ◽  
Endemic Plants ◽  
Phytochemical Screening ◽  
Aromatic Plants ◽  
Herbal Products ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Fragrance Compounds

Aromatic plants are a reservoir of secondary metabolites that are responsible for the plants' medicinal efficacy and a major source of aromatic compounds and essential oils.  Now a day, there is a renewed interest in the plant kingdom in the search for novel bioactive and fragrance compounds as demands for herbal products are increasing enormously. In the present study two endemic plants, Pinda concanensis and Heracelum grande from Western Ghats were analyzed for secondary metabolites by using different plant parts (roots, leaves, and seeds) and by using range of solvent from polar to non-polar.  Glycosides, terpenoids, tannins, flavonoids, saponins, steroids, and alkaloids were found in methanolic extracts of Pinda concanensis root, leaf, and seeds and methanolic extracts of seeds of Heracelum grande. While extracts of chloroform and petroleum ether exhibits presence of some of them. Obtained results show the importance of both the plants as an important source of biologically active molecules for pharmaceuticals and cosmetic industries. Keywords: Phytochemical screening, secondary metabolites, biologically active compounds.

scholarly journals Phytochemical Analysis of Muntingia calabura Extracts Possessing Anti-Microbial and Anti-Fouling Activities

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Singh R. ◽  
Iye S. ◽  
Prasad S. ◽  
Deshmukh N. ◽  
Gupta U. ◽  
...  
Keyword(s):  
Traditional Medicine ◽  
Microbial Activity ◽  
Plant Extracts ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Hemagglutination Activity ◽  
Standard Drug ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Muntingia Calabura

The present study was carried out to determine the possible antifouling and anti microbial activity of plant extracts prepared from Muntingia calabura and phytochemical screening was demonstrated. The extracts from the following plant parts like stem, leaves, fruits, flower and stems were prepared in different aqueous solvents like ethanol, methanol, acetone, acetonitrile and water. The highest antimicrobial potentials were observed for the methanolic extracts against K. pneumonia, B. subtilis, B. megaterium and P. aeruginosa. Its efficacy was comparable to the standard drug, ampicillin. Significant amount of tannins, alkaloids, steroids and flavonoids were found. Interestingly, the present study showed anti fouling effect against pathogenic biofilm forming bacteria. These extracts also contain high hemagglutination activity. The present study provides evidence that solvent extract of M. calabura contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT PROPERTIES OF LEAF EXTRACTS OF CARICA PAPAYA

2020 ◽  
pp. 58-62
Author(s):  
NANDINI G ◽  
GOPENATH TS ◽  
NAGALAMBIKA PRASAD ◽  
MURUGESAN KARTHIKEYAN ◽  
ASHOK GNANASEKARAN ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Biologically Active ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Isolation And Purification ◽  
Chain Reactions ◽  
Methanolic Extracts

Objective: The objective of the present study aimed at investigating the phytochemical and antioxidant properties of Carica papaya leaf extracts. Methods: As phytochemicals are biologically active compounds and a powerful group of plant chemicals, believed to stimulate the immune system along with antioxidants, the molecules which hinder oxidation of other molecules by the process of inhibiting or by generating the oxidizing chain reactions and preventing diseases. The total phenolic content (TPC) was determined by Folin-Ciocalteu method and total flavonoid contents (TFC) were determined aluminum chloride method and antioxidant by 2,2,1-diphenyl-1-picrylhydrazyl method. Results: The results of phytochemical screening revealed the presence of bioactive compounds such as alkaloid, carbohydrates, and amino acids and TPC and TFC varied among the different solvent extracts, in which methanolic extracts showed highest amount of phytochemicals and TPC and TFC and antioxidants compared to other solvents. Conclusion: The isolation and purification of specific bioactive compound may account as natural and promising medicines in exploration of new drug.

scholarly journals PHYTOCHEMICAL ANALYSIS OF Turnera diffusa WILLD

2020 ◽  
Vol 8 (11) ◽  
pp. 402-411
Author(s):  
Maria Regina de Oliveira Silva ◽  
Maria Herbênia Lima Cruz Santos ◽  
Xirley Pereira Nunes ◽  
Emanuel Ernesto Fernandes Santos ◽  
Eugênio Bispo da Silva Júnior
Keyword(s):  
Secondary Metabolites ◽  
Folk Medicine ◽  
Phytochemical Analysis ◽  
Pharmacological Effects ◽  
Phytochemical Screening ◽  
Turnera Diffusa ◽  
Pharmacological Potential ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
Chemical Class

Turnera diffusa WILLD is a plant used in folk medicine as a natural stimulant, and the objective of this research is to perform the phytochemical analysis of the secondary metabolites of the damiana of occurrence in the Juazeiro region, northern Bahia - Brazil. A phytochemical screening was performed from three Crude Ethanol Extracts (BSE), EEB1: leaves and flowers; EEB2: stems and EEB3: root. A thin analytical layer chromatography was performed using specific developers to detect each chemical class. Alkaloids, coumarins, anthranic derivatives, phenolic compounds, mono, sequi and diterpenes, naphthoquinones, triterpenes and steroids, saponins, hydrolyzed tannins and xanthines were identified in the three extracts. Only in the BSE of leaves and flowers observed the presence of antroquinones and aglycones. The secondary metabolites identified in the study evidence the pharmacological potential of Turnera diffusa. Phytochemical screening concluded the presence of fourteen classes of secondary metabolites in damiana, indicating important medicinal potential with pharmacological effects reported in the literature.

scholarly journals Allelopathic Potential of Ethanolic Extract and Phytochemical Analysis of Paspalum maritimum Trind

2019 ◽  
Vol 37 ◽  
Author(s):  
J.C. PEREIRA ◽  
C.L.A. PAULINO ◽  
L. ENDRES ◽  
A.E.G. SANTANA ◽  
F.R.S. PEREIRA ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Condensed Tannins ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Allelopathic Effect ◽  
Active Fraction ◽  
Allelopathic Potential ◽  
Plant Parts ◽  
Allelopathic Effects ◽  
Donor Species

ABSTRACT: Allelopathy is defined as the ability of certain plants and microorganisms to interfere with the metabolism of other species through substances released into the environment, being an alternative to control weeds and diseases. In this context, this study aimed to evaluate the allelopathic potential and identify groups of secondary metabolites of Paspalum maritimum Trind. The species Lactuca sativa, Digitaria insularis, Emilia coccinea, and Portulaca oleracea were used as recipient plants. The ethanolic extract was obtained from shoot and root of donor species to evaluate the allelopathic potential. Potentially allelopathic effects were evaluated by means of germination tests, germination speed index, and initial seedling growth. A phytochemical analysis of the extract was also performed to identify the secondary metabolites. The ethanolic extract from both plant parts had an allelopathic effect on recipient species. The presence of condensed tannins, chalcones and aurones, flavonones, steroids, and saponins were detected in the most active fraction of the shoot of P. maritimum.

scholarly journals In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela

2016 ◽  
Vol 64 (4) ◽  
Author(s):  
Alexis Buitrago ◽  
Janne Del Carmen Rojas ◽  
Yonel Peñalosa
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Methanol Extracts ◽  
Phenol Derivatives ◽  
Methanolic Extracts ◽  
High Concentrations

Vismia genus is distributed mainly in tropical and subtropical regions of Central, South America and some areas of Africa. According to previous investigations, antioxidant potential of Vismia species might be related to anthrones, anthraquinones, flavonoids and phenol derivatives biosynthesized by these plants. The aim of present study is to evaluate the free radical scavenging capacity, total phenolic and flavonoids content as well as the qualitative phytochemical screening of methanol extracts obtained from Vismia macrophylla (VM) and Vismia baccifera (VB) collected in Táchira and Mérida state, Venezuela. Phytochemical screening of VB and VM methanolic extracts carried out using various chemical assays revealed an abundant presence of anthraquinones in both species analyzed. Glycosides were also present while flavones and dehydroflavones were observed abundantly in VB but moderated in VM. Triterpenes were also detected and steroids showed to be abundant in VM but moderate in VB. On the other hand, antioxidant capacity measured by the DPPH radical scavenging assay showed that VM possesses a stronger antioxidant activity than VB with IC50 5.50 µg/mL. Phenol and flavonoid assays also revealed that methanol extracts of both VM and VB contain high concentrations of these metabolites. A relationship between the antioxidant activity, total phenol and flavonoids content of the extracts analyzed was demonstrated in present investigation since those samples with higher phenolic concentrations showed likewise higher antioxidant activity.

scholarly journals Endophytic Fungi from Alstonia boonei De Wild and Greenwayodendron suaveolens (Engl. and Diels) Verdc. subsp. Suaveolens Possess Inhibitory Activity against Pneumonia Causing Bacteria

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Paola Cynthia Emoh Demeni ◽  
Patrick Hervé Diboue Betote ◽  
Christelle Wayoue Kom ◽  
Eric Ngalani Tchamgoue ◽  
Esther Del Florence Ndedi Moni ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Methanolic Extracts ◽  
Bactericidal Effects ◽  
Inhibition Zones

This study evaluated the antibacterial efficacy of methanolic extracts of isolated endophytic fungi from stem barks and leaves of Alstonia boonei De Wild and Greenwayodendron suaveolens (Engl. and Diels) Verdc. subsp. Suaveolens against Klebsiella pneumoniae ATCC 43816, Haemophilus influenzae ATCC 49247, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 35218, responsible for causing pneumonia. The endophytic fungi were isolated and characterized in the Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA), and Czapek Dox Agar (CDA) media. The fungi and their methanolic extracts were tested for in vitro antibacterial potential by antagonistic assay for endophytic fungi against bacterial pathogens and microdilution method. The phytochemical screening of extracts was carried out according to the colorimetric and precipitation methods to reveal the presence of secondary metabolites. The results showed that 24 macroscopically and microscopically distinct endophytic fungi were isolated, identified, and stored. These endophytic fungi possessed antibacterial activity against the selected bacterial strains with inhibition zones ranging from 7.00 to 25.00 mm. The endophytic fungi GS15 and AB24 have presented the inhibitions zones of 20.33 mm and 25.00 mm, respectively, and these were better than the ones obtained for Levofloxacin®. The endophytes with inhibition zones greater than 10 mm were used for extraction of their secondary metabolites. The endophytic fungi extracts showed antibacterial activity with the minimum inhibitory concentrations (MICs) ranging from 6.25 × 10−4 to 2 × 10−2 g/L and the minimum bactericidal concentrations (MBCs) ranging from 2.5 × 10−3 to 2 × 10−2 g/L. The endophytic fungi GS15 extract was the most effective extract; it showed bactericidal effects on the tested bacterial strains. The phytochemical screening of the extracts revealed the presence of secondary metabolites classes, responsible for causing the obtained antibacterial activity. Thus, the endophytic fungi methanolic extracts from A. boonei and G. suaveolens have the potential to inhibit the growth of bacteria responsible for nosocomial pneumonia.

scholarly journals Study on ruminant tick infestation, phytochemical analysis and in vitro acaricidal effect of Calpurnia aurea and Otostegia integrifolia extracts on Amblyomma variegatum

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Jelalu Kemal ◽  
Sisay Alemu ◽  
Biniam Tsegaye ◽  
Nateneal Tamerat
Keyword(s):  
Tick Infestation ◽  
Cross Sectional Study ◽  
Amblyomma Variegatum ◽  
Phytochemical Analysis ◽  
In Vitro Test ◽  
Phytochemical Screening ◽  
Cross Sectional ◽  
Acaricidal Efficacy ◽  
Methanolic Extracts

Ticks limit the productivity of livestock through decreased production, reproduction, increased mortality, downgrading and rejection of hides and skin. A cross-sectional study was conducted to estimate the prevalence of tick infestation in ruminant while experimental study was used to evaluate the in-vitro acaricidal efficacy of methanolic extracts: Calpurnia aurea and Otostegia integrifolia and the phytochemicals present in those extracts at different concentrations (200, 100, 50, 25, 12.5 and 6.25 mg/ml) against Amblyomma variegatum. Adult immersion was used for the in-vitro acaricidal efficacy test and plant extracts were subjected to qualitative phytochemical screening for the presence or absence of secondary metabolites using standard procedures. Out of the 160 goats, 152 sheep and 121 cattle, 23 (14.4%), 44 (28.9%) and 28 (23.1%) were found to be positive for tick infestation, respectively. The incidence of tick infestation was significantly different (p<0.01) among ruminants. Five tick spp. were identified: A. variegatum, A. gemma, R. decoloratus, R. evertsi evertsi and R. pulchellus. Extract of C. aurea and O. integrifolia was found to contain alkaloids, saponins, phlobatannin, steroids, phenolic, flavonoids, glycosides and tannins. However, both plants were found negative for triterpens. Extracts of C. aurea and O. integrifolia at 200 and 100 mg/ml concentrations showed a significantly higher (p<0.05) acaricidal activities compared to other treatments at 24 hrs post exposure. Mortality of ticks was increased with the increased dosage (concentration) and exposure time after treatment. Extracts of C. aurea showed a significantly higher (p<0.05) tick mortality (52%) compared to those of O. integrifolia (27%). This is a promising finding to have alternative means of treatment and to substitute the use of synthetic drugs which have a wide spread drug resistance especially in developing countries like Ethiopia. Key words: Calpurnia aurea; in-vitro test; Otostegia integrifolia; Phytochemical screening; Tick infestation

scholarly journals Preliminary analysis of a neotropical plant extract antiviral potential against Chikungunya and Mayaro viruses

2021 ◽  
Author(s):  
Ellen Caroline Feitoza Pires ◽  
Francini Pereira Da Silva ◽  
Karoline Schallenberger ◽  
Bruna Saraiva Hermann ◽  
Larissa Mallmann ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
In Silico ◽  
Preliminary Analysis ◽  
Plaque Assay ◽  
Traditional Healers ◽  
In Vitro Assays ◽  
Phytochemical Analysis ◽  
Methanolic Extracts ◽  
Computational Predictions

Chikungunya and Mayaro fevers are viral infectious diseases, without vaccine or treatment, that causes fever and arthralgia. Establishing novel antiviral tools capable of preventing or treating Chikungunya virus (CHIKV), and Mayaro virus (MAYV) infections are needed. The use of plant-based compounds that affect the replication cycles of these viruses has been proposed as a promising strategy. Chiococca alba (L.) Hitchc. is a neotropical plant used by Yucatec Mayas traditional healers, mainly, as antipyretic and antirheumatic. To evaluate the potential of C. alba methanolic extracts against CHIKV and MAYV through preliminary analysis in vitro and in silico . The cytotoxicity profile of two C. alba roots methanolic extracts in Vero cells was performed by lysosomal viability using the neutral red assay, and the antiviral potential was determined by plaque assay. We further assessed, through in silico computational predictions, the possible interactions between the active site of the nsP2 proteases of these viruses with some secondary metabolites present in C. alba extracts, identified by High-Performance Liquid Chromatography (HPLC). Our partial phytochemical analysis revealed the presence of flavonoids, and phenolic acids in the C. alba extracts. Our in vitro assays showed that both C. alba extracts inhibited more than 70% of CHIKV and MAYV activities at 60 µg/mL concentration. Based on our in silico computational predictions, the flavonoids naringin and vitexin showed the greatest affinity energies with the CHIKV and MAYV nsP2 proteases, revealing the great potential of these compounds as viral inhibitors. The findings described here indicates that C. alba extracts, or their secondary metabolites, as a potential source of novel antiviral compounds.

scholarly journals Phytochemical screening and antioxidant activity of wild mushrooms growing in tropical regions

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
ELISA HERAWATI ◽  
RICO RAMADHAN ◽  
FARIDA ARIYANI ◽  
MARJENAH MARJENAH ◽  
IRAWAN WIJAYA KUSUMA ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Phytochemical Analysis ◽  
Test Results ◽  
Phytochemical Screening ◽  
Wild Mushrooms ◽  
Tropical Regions ◽  
Color Changes ◽  
Ic50 Value ◽  
In The Wild

Abstract. Herawati E, Ramadhan R, Ariyani F, Marjenah, Kusuma IW, Suwinarti W, Mardji D, Amirta R, Arung ET. 2021. Phytochemical screening and antioxidant activity of wild mushrooms growing in tropical regions. Biodiversitas 22: 4716-4721. This study aims to determine the presence of secondary metabolites which generally have bioactivity that is antioxidant with phytochemical tests and antioxidant tests in Auricularia auricula, Schyzophyllum commune, Microporus xanthopus, and Trametes versicolor fungi that grow in the wild. Phytochemical analysis was carried out by testing color changes and modifications, while antioxidant tests were carried out by using a UV-Vis spectrophotometer and a DPPH solution (1,1-diphenyl-2-picrylhydrazyl). This research found that edible wood fungus that grows, wild such as A. auricula, contains secondary metabolites, namely flavonoids, triterpenoids, saponins, and tannins, but does not contain steroids, alkaloids, carotenoids, or coumarin, while S. commune contains secondary metabolites, namely flavonoids, steroids, tannins, and coumarin, but does not contain triterpenoids, alkaloids, carotenoids, or saponins and non-edible wood fungus, such as M. xanthopus, contains secondary metabolites, namely, flavonoids, saponins, tannins, coumarin, and steroids, but does not contain triterpenoids, alkaloids, or carotenoids, while T. versicolor contains compounds, secondary metabolites, flavonoids, saponin, tannins, coumarin, and triterpenoids, but does not contain steroids, alkaloids, or carotenoids. The antioxidant test results are based on IC50 calculations; it is known that the IC50 value of A. auricula is 499.25 g/mL, while S. commune is 121.37 g/mL, M. xanthopus is 251.20 g/mL, and T. versicolor is 493.04 g/mL. Based on the research results, edible and inedible wood mushroom extracts contain several phytochemicals and show potential for antioxidant activity.

scholarly journals Secondary metabolites in plants: main classes, phytochemical analysis and pharmacological activities

Bionatura ◽  
2019 ◽  
Vol 4 (4) ◽  
pp. 1000-1009
Author(s):  
Irina Francesca González Mera ◽  
Daniela Estefanía González Falconí ◽  
Vivian Morera Córdova
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolism ◽  
Chemical Compounds ◽  
Biological Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Biological Functions ◽  
Pharmacological Activities

Plants are an essential source of chemical compounds with different biological properties that man can use to his advantage. These substances are mainly produced as a result of chemical conversions of secondary metabolism. This article reviews the main classes of secondary metabolites that synthesize plants as well as their characteristics and their biological functions. Examples are provided for each of the classes. Emphasis is placed on the methods of extracting secondary metabolites and phytochemical screening, as well as on the main pharmacological activities described for the MS.

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