scholarly journals Isolation and characterization of bioactive compound anthraquinone from methanolic extract of Boerhavia diffusa linn.

2018 ◽  
Vol 8 (5-s) ◽  
pp. 332-337
Author(s):  
U Kanagavalli ◽  
A. Mohamed Sadiq
Keyword(s):  
Thin Layer ◽  
Column Chromatography ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Bioactive Compound ◽  
Separation And Purification ◽  
Active Components ◽  
Isolation And Characterization ◽  
Boerhavia Diffusa

Boerhavia diffusa Linn. (Nyctaginaceae), commonly known as ‘Punarnava’ is a perennial creeping herb widely studied and has a long history of uses by the tribal people and in Ayurvedic and Unani medicines. Our previous study showed that in different extracts of Boerhavia diffusa (ethanol, methanol, petroleum ether, aqueous and hexane), methanolic extract had significant anti-oxidant activity, but the active components present in that extracts are still unclear. In this study, Boerhavia diffusa Linn was investigated for the bioactive compounds present in its methanolic extract. Separation and purification of the compounds in the most active methanol extract was done using a combination of column chromatography and thin layer chromatography. The compound was identified using gas chromatography and mass spectrophotometry. The resulted compound anthraquinone which is also called as 9, 10 anthracenedione. In addition to their known use as natural dyes, it have several biological activities like antitumor, antiinflammatory,  diuretic , antiarthritic, antifungal, antibacterial,  antimalarial and antioxidant activities . This justifies the use of this plant in traditional medicine and indicates a promising potential for the development of medicinal agents from Boerhavia diffusa. Keywords: Antioxidant, Boerhavia diffusa Linn, anthraquinone, column chromatography, Thin layer Chromatograhy, GC-MS.

scholarly journals Isolation and Characterization of Bioactive Components Derived from Whole Plant of Selaginella bryopteris

2020 ◽  
Vol 10 (4-s) ◽  
pp. 127-131
Author(s):  
Abhilasha Jain ◽  
Savita Shrivastava
Keyword(s):  
Crude Extract ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Herbal Remedies ◽  
Active Components ◽  
Protein Amino Acid ◽  
Isolation And Characterization ◽  
Whole Plant ◽  
Selaginella Bryopteris

Natural products and herbal remedies used in traditional folklore medicine have been the source of many medically beneficial drugs because they elicit fewer side effects, relatively cheap, affordable and claimed to be effective. However, in order to make these remedies acceptable to modern medicine, there is a need to scientifically evaluate them to identify the active principles and to understand their mechanism of action. Selaginella bryopteris (S. bryopteris) is a pteridophytic plant belongs to the family selaginellaceae. Its familiar name is sanjeevani booti. The aim of the present investigation was isolation and characterization of active components derived from whole plant of S. bryopteris. The plant was extracted with petroleum ether, ethyl acetate and methanol solvent. The preliminary phytochemical results revealed that alkaloids, carbohydrates, reducing sugar, flavonoids, protein, amino acid, tannin and phenolic compound as active constituents in methanolic extract of S. bryopteris. The total phenolics content of whole plant of methanolic extract was (184.13±0.416 mg/gm), followed by flavonoids (89.67±2.516mg/gm). The quantification and the identification of compounds in the crude extract and active bands isolated by preparative TLC were accomplished using spectroscopic analysis. The most important compounds amentoflavone identified in the crude extract appreciable amounts may account for its various biological activities. Keywords: Selaginella bryopteris, Isolation, Plant extraction, Phytochemical

scholarly journals Chemical and Biological Investigations of Cerbera odollam Gaertn

2018 ◽  
Vol 16 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Monica Sharfin Rahman ◽  
Adnan Faisal ◽  
Choudhury Mahmood Hasan ◽  
Monira Ahsan ◽  
Mohammad Mehedi Masud
Keyword(s):  
Carbon Tetrachloride ◽  
Secondary Metabolites ◽  
Petroleum Ether ◽  
Chromatographic Separation ◽  
Methanolic Extract ◽  
Scientific Basis ◽  
Separation And Purification ◽  
Isolation And Characterization ◽  
Soluble Fractions

The present article describes the isolation and characterization of secondary metabolites from the leaves of Cerbera odollam as well as bioactivities of the crude methanolic extract and its petroleum ether (PE), carbon tetrachloride (CTC), chloroform (CF) and aqueous (AQ) soluble fractions. In this investigation, successive chromatographic separation and purification yielded β-amyrin (CO-01), lupeol (CO-02), β-sitostenone (CO-03) and triticusterol (CO-04). The study also indicated the antioxidant, cytotoxic, antimicrobial, membrane stabilizing activities and poor thrombolytic activities. Thus, the present study endeavors to provide scientific basis to establish Cerbera odollam as a potential medicinal plant.Dhaka Univ. J. Pharm. Sci. 16(2): 179-186, 2017 (December)

scholarly journals ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS FROM ETHYL ACETATE AND METHANOL EXTRACTS OF GARCINIA DAEDALANTHERA PIERRE LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 276
Author(s):  
Anisa Puspitaningrum ◽  
Berna Elya ◽  
Katrin .
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Spectrum Analysis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Uv Light ◽  
Antioxidant Compounds ◽  
Isolation And Characterization ◽  
Dpph Method

Objective: This study aimed to isolate and characterize the compounds responsible for the high antioxidant activities of the ethyl acetate and methanolextracts of Garcinia daedalanthera Pierre leaves.Methods: In this study, the ethyl acetate extract was obtained by column chromatography, and the methanol extract was obtained by vacuum columnchromatography. The mobile phase comprised n-hexane, ethyl acetate, and methanol with increased polarity. Antioxidant activity was examined usingthe 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The fraction with the highest antioxidant activity was purified through column chromatography,recrystallization, and preparative thin-layer chromatography. This fraction, termed the isolate of B, was identified using DPPH and AlCl3, and itsantioxidant activity was quantitatively tested.Results: From this research, 21.7 mg of the isolate of B were obtained with an IC50 of 5.82 μg/mL. Identification using an AlCl3 sprayer producedyellow phosphorescent spots under UV light. UV-Vis spectrum analysis revealed the presence of an aromatic compound and conjugated double bonds.Infrared spectrum analysis revealed the presence of −OH, C–H alkane, C=C aromatic, C=O, and C-O-C groups.Conclusion: Based on this research, 21.7 mg of the isolate of B was derived through fractionation of the methanol extract, and this isolate exhibitedantioxidant activity with an IC50 of 5.82 μg/mL. The isolate of B was considered to be a flavonoid, as it was fluorescent under UV light (366 nm) afterbeing sprayed with AlCl3 reagent.

scholarly journals Antioxidant and antityrosinase activities of milky white mushroom

2019 ◽  
Vol 48 (4) ◽  
pp. 1065-1073
Author(s):  
Nuhu Alam ◽  
Md Maniruzzaman Sikder ◽  
Md Abdul Karim ◽  
Sheikh Md Ruhul Amin
Keyword(s):  
Gallic Acid ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Reducing Power ◽  
Positive Control ◽  
Hot Water ◽  
Biochanin A ◽  
Calocybe Indica ◽  
Β Carotene

It was intended to investigate the biological activities of different solvent extracts of the fruiting bodies of Calocybe indica (P & C). The bioactive compounds were extracted using acetone, methanol, and hot water. The antioxidant activities of three different extracts at 0.5-20.0 mg/ml of C. indica on β-carotene-linoleic acid ranged from 65.83 - 92.56, 62.79 - 93.06 and 61.42 - 92.12%, respectively. The highest (2.825) reducing power inhibition was recorded in methanolic extract, while the lowest (2.332) was in hot water at the concentration of 8 mg/ml. The free radicle scavenging activity was more pronounced in methanolic extract. Chelating effect of C. indica was significantly strong as compared to positive control. The xanthine oxidase and tyrosinase inhibitory activity of the acetone, methanol, and hot water extracts of C. indica increased with increasing concentration. Thirteen phenolic compounds i.e. biochanin-A, caffeic acid, chlorogenic acid, ferulic acid, formononetin, gallic acid, hesperetin, homogentisic acid, naringenin, naringin, protocatechuic acid, resveratrol and vanillin were detected from acetonitrile-hydrochloric acid extract. The result indicated that the maximum and minimum concentrations were recorded for gallic acid (29 μg/g) and formononetin (10 μg/g). Thus, it could be suggested that C. indica has the potential to be used as antioxidant and tyrosinase protection system of the human body against oxidative damage and other complications.

scholarly journals Evaluation of Phytochemical, Antimicrobial and Antioxidant Capacities of Pennisetum purpureum (Schumach) Extracts

2020 ◽  
pp. 1-14
Author(s):  
I. R. Jack ◽  
P. D. Clark ◽  
G. I. Ndukwe
Keyword(s):  
Antifungal Activity ◽  
Vitamin C ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Pennisetum Purpureum ◽  
Diffusion Method ◽  
Methanolic Extracts ◽  
Test Organisms

Medicinal plants are valuable natural sources for the development of potentially safe drugs. The biological activities associated with these plants are due to the presence of certain phytochemicals that act individually or synergistically. Thus, this study examined the phytochemical components, antioxidant and antimicrobial activities of n-hexane, ethylacetate and methanolic extracts of Pennisetum purpureum (Schumach). Qualitative and quantitative phytochemical assays of P. purpureum showed the presence of alkaloids (0.004%), saponins (0.002%), flavonoids (0.021%), steroids, terpenoids and glycosides (0.008%). Methanol, n-hexane and ethylacetate extracts of P. purpureum were examined for antimicrobial activity using the disc diffusion method. Six microbial strains were exposed to six different concentrations of each extracts; 200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, 12.5 mg/ml and 6.25 mg/ml. The three extracts demonstrated varied concentration-dependent antimicrobial activities against the test organisms. The methanolic extract showed antibacterial activity against E. coli, S. aureus, B. cereus and antifungal activity against T. mentagrophyte and A. niger. Among all extracts, the methanolic extract of P. purpureum exhibited relatively strong antifungal activity against A. niger (10.3±0.12 mm) when compared to the standard antifungal agent, fluconazole (13.9±0.12 mm). Furthermore, antioxidant activities were spectrophotometrically studied using vitamin C as standard; methanol and ethylacetate extracts of P. purpureum showed pronounced scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH); and had a potent reductive ability on ferric ion and phosphomolybdate. However, only the non-polar extract of P. purpureum showed a non-significant correlation and significant differences when compared to vitamin C. Antioxidant activities of the plant extracts were observed in the order of methanol >ethylacetate> n-hexane extracts. The results showed that P. purpureum contains phytochemicals that significantly contributed to the observed antimicrobial and antioxidant abilities of the plant and could be used as a potential source for the development of novel therapeutic drugs.

scholarly journals Isolation, Characterization and Phytochemical Evaluation of Active Compound Coumarin from Oldenlandia corymbosa (Linn)

2021 ◽  
pp. 328-335
Author(s):  
Sonja Jose ◽  
B. Jayakar
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Active Compound ◽  
High Performance ◽  
Bioactive Compound ◽  
Tropical Regions ◽  
Isolation And Characterization ◽  
Iridoid Glucosides ◽  
Whole Plants ◽  
Layer Chromatography

Medicinal plants have served through ages, as a constant source of medicaments for the exposure of a variety of diseases. Plants are known to provide cures for various human illnesses and are a rich source of phyto constituents having diversified pharmacological properties. Oldenlandia corymbosa (L.) (syn. Hedyotis corymbosa Lam., Thai name Ya-Lin-Ngu), a member of the family Rubiaceae, is widely distributed in tropical regions of Asia. The decoction of whole plants is used in traditional medicine for antipyretic purposes to decrease body temperature. Pharmacologically, the anti-inflammatory, antioxidant, and hepatoprotective properties of plant extracts have been reported. This plant is well known to contain mainly iridoid glucosides. The whole plant contains reducing sugars, amino acids, flavonoids, steroids, glycosides and alkaloids. The present aim of the study is to extraction and isolation of bioactive compound from Oldenlandia corymbosa (L.). The isolation and characterization analysis included, Thin Layer Chromatography, High Performance Thin Layer Chromatography, GC-MS and Spectroscopy studies (IR, NMR, and MASS). The presences of coumarin in the methanolic fraction revealed that the isolated constituent is most active compound. Isolation of bioactive compound coumarin may help in identification of various pharmacological activities and carrying out further research in Oldenlandia corymbosa  (L.).

scholarly journals Effects of nitrogen application on phytochemical component levels and anticancer and antioxidant activities of Allium fistulosum

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11706
Author(s):  
Chen Zhao ◽  
Zhongjian Wang ◽  
Rongzong Cui ◽  
Le Su ◽  
Xin Sun ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Nitrogen Concentration ◽  
Fertilizer Application ◽  
Nitrogen Fertilizers ◽  
Allium Fistulosum ◽  
Active Components ◽  
Nitrogen Levels ◽  
Anticancer Properties ◽  
High Nitrogen Level

Background Allium fistulosum L. has good nutritional value and is cultivated worldwide as an efficacious traditional medicinal plant. Its biological activities are attributable to its phytochemicals. Nitrogen is an essential nutrient for plant growth and development; however, the effect of nitrogen levels on the level of active components in this species is not well understood. Methods In this study, using urea fertilizer, we investigated the effects of different nitrogen levels (N0, N1, and N2 at 0, 130, and 260 kg/ha, respectively) on the phytochemical constituents , and antioxidant and anticancer properties of A. fistulosum. Results The results suggested that nitrogen fertilizers have a significant effect on the level of total phenols and flavonoids. The analysis of the antioxidant capacity revealed that the lowest IC50 values corresponded to plants treated with the highest nitrogen concentration. Anticancer activity was investigated against cancer cell lines (HeLa and HepG2), and the extracts of A. fistulosum treated with a high nitrogen level showed the highest antiproliferative effect. Collectively, our results suggest that nitrogen fertilizer application enhanced the quality of A. fistulosum, particularly its health benefits.

scholarly journals Nutritional and Bioactive Characterization of Sicana odorifera Naudim Vell. Seeds By-Products and Its Potential Hepatoprotective Properties in Swiss Albino Mice

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1351
Author(s):  
Silvia Caballero ◽  
Laura Mereles ◽  
Alberto Burgos-Edwards ◽  
Nelson Alvarenga ◽  
Eva Coronel ◽  
...  
Keyword(s):  
Environmental Sustainability ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Essential Fatty Acids ◽  
Antioxidant Properties ◽  
Swiss Albino Mice ◽  
Quercetin Derivatives ◽  
Albino Mice ◽  
By Products

The “Kurugua” (Sicana odorifera) is a native fruit that demonstrates attractive nutritional, coloring, flavoring, and antioxidant properties. The main by-products from the processing and consumption of kurugua fruit are epicarp and seeds. In this work, the properties of the seeds of S. odorifera were evaluated. The nutritional composition of the fruit seeds was determined through AOAC official methods and UHPLC-ESI-MS/MS profiling. The antioxidant activities were determined using in vitro methods, and the acute toxicity and hepatoprotective properties were investigated in Swiss albino mice. Quercetin derivatives and cucurbitacins were the main phytochemicals in the seeds’ methanolic extract and demonstrated some biological activities. GC-MS analysis revealed the essential fatty acids linolenic and linoleic as the main compounds present in seeds oil. The methanolic extract significantly reduced the serum levels of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) in mice with induced hepatotoxicity (GPT p < 0.05; GOT p < 0.001) at the minor concentration tested (100 mg/kg EMSo). The results suggest that the S. odorifera seeds as by-products show potential use as a source of phytochemicals and in the production of oils with application in food supplements and nutraceuticals. Their integral use could contribute to waste reduction from kurugua fruits processing within the food safety and environmental sustainability framework.

Separation and Purification of Ganglioside from Sika Deer Antler

2013 ◽  
Vol 850-851 ◽  
pp. 1137-1140 ◽  
Author(s):  
Ni Na Bao ◽  
Yong Guang Yin ◽  
Quan Kai Wang
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Sika Deer ◽  
Soluble Fraction ◽  
Biological Activities ◽  
Separation And Purification ◽  
Deer Antler ◽  
Extraction Technology ◽  
Inhibitory Activities

In this paper, a ganglioside was extracted using acetone from the lipid soluble fraction of sika deer antler which was extracted by supercritical CO2 extraction technology. The crude ganglisode was purified using silica gel column chromatography, was identified using TLC and was characterized by employing IR. The biological activities show ganglioside has obvious inhibitory activities, and the pure one is rather good than the crude one.

scholarly journals TLC analysis and antioxidant activity of garden egg leaves

2020 ◽  
Vol 12 (1) ◽  
pp. 443-448
Author(s):  
U.A. Umar ◽  
L.G. Hassan ◽  
K.L. Maradun
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Solanum Melongena ◽  
Solvent System ◽  
Antioxidant Compounds ◽  
Isolation And Characterization ◽  
Layer Chromatography

The Solanum melongena is used traditionally to treat different diseases such as cancer, atherosclerosis, and inflammation. This study is aimed at investigating the thin-layer chromatography analysis and antioxidant activity of n-hexane, acetone and methanol leaves extract of Solanum melongena. Sequential extraction with solvents of increasing polarity was carried out using the n-hexane, ethyl acetate and methanol. The thin-layer chromatography of the extracts carried out with different solvent system. Antioxidant activity was evaluated quantitatively using DPPH (2, 2-diphenyl-2-picrylhydrazyl) for its free radical scavenging ability. The results of thin-layer chromatography revealed some spots with Rf values in the respective extracts, n-hexane (0.31, 0.42, 0.59, 0.65, 0.72, 0.85, 0.92), acetone (0.97, 0.92, 0.88 and 0.59) and methanol (0.72, 0.83, 0.86, 0.94 and 0.96). The extracts exhibit strong antioxidant activities as radical scavengers, indicating that they have strong proton donating abilities. The results from this research show credence to the traditional application of the plant. Further research is recommended on the isolation and characterization of the antioxidant compounds from the plant. Key words: DPPH (2, 2-diphenyl-2-picrylhydrazyl), Thin layer chromatography, Reactive Oxygen Species (ROS)  

Sign in / Sign up

Export Citation Format

Share Document