scholarly journals PREVALENCE OF RUBELLA IGG ANTIBODIES AMONG PRODUCTIVE-AGE ‎WOMEN IN AL-MAHWEET GOVERNORATE, YEMEN

2020 ◽  
Author(s):  
Amatalkhaliq Abdullah Gobara'a ◽  
Wadhah Hassan Edrees ◽  
Wadee Abdullah Al-Shehari ◽  
Anwar Al-Madhagi ◽  
Khaled Al-Moyed ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Peer Review ◽  
Rubella Virus ◽  
Reproductive Age ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Rubella Infection ◽  
Significant Difference ◽  
History Of ◽  
E Mail

Background: Rubella is an infectious viral disease that caused by the Rubella virus. The incidence of rubella infection in women during pregnancy leads to complications for fetus development and cause congenital rubella syndrome or fetal death. This study aimed to determine the prevalence rate of rubella among reproductive-age women in Al-Mahweet, Yemen. Methods: A cross sectional study was conducted among females attending healthcare centers and schools from July 2007 to June 2008. Blood samples were collected individually from 270 females aged 15-35 years and the rubella virus IgG antibody was quantitated by enzyme-linked immunosorbent assay (ELISA). The needed data were collected by using a pretested questionnaire and analyzed by statistical program. Results: Overall, 197(73%) had IgG-positive antibody to rubella and 73(27%) had IgG-negative antibody to rubella. The highest rate of anti-rubella IgG was among females aged 15-25 years and the lowest was among the females aged 31-35 years. Similarly, the higher rate was among females living in urban area compared to females from rural area. The statistically significant difference was found between most educational levels and positive results of anti-rubella IgG. With regard to marital status, the most frequent of rubella antibody was 72.3%, 73.1%, and 75.0%, respectively, recorded among single, married, and divorced females. The non-pregnant women had (73.7%) more incidence to rubella IgG antibody than pregnant (66.7%). 82.2% of IgG- positive women had no history of stillbirth and 65% had a history of stillbirth. Conclusions: Most of the enrolled females had immunity against rubella virus, but still a significant percentage were susceptible to rubella infection. Thus, it is essential to introduce of rubella vaccine to control and prevent the rubella virus circulating among the community. Peer Review History: Received 20 July 2020; Revised 11 August; Accepted 22 August, Available online 15 September 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Name: Dr. (Mrs) Amaka A. Mgbahurike Affiliation: University of PortHarcourt, Nigeria E-mail: [email protected]   Name: Dr. Tamer Elhabibi Affiliation: Egyptian Russian University, Egypt E-mail: [email protected]   Name: Rawaa S. Al-Kayali Affiliation: Aleppo university, biochemistry and microbiology department, Syria E-mail: [email protected]   Name: Rola jadallah Affiliation: Arab American University, Palestine E-mail: [email protected]   Comments of reviewer(s): Similar Articles: THE ASSOCIATION OF EPSTEIN-BARR VIRUS ANTIBODIES WITH RHEUMATOID ARTHRITIS AMONG YEMENI PATIENTS IN SANA’A CITY SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY- YEMEN

scholarly journals Sero-prevalence of unvaccinated adolescent girls susceptible to rubella virus infection seen in a tertiary care hospital of Patna, Bihar, India

2020 ◽  
Vol 9 (5) ◽  
pp. 1972
Author(s):  
Dipali Prasad ◽  
Nidhi Prasad ◽  
Vidyut Prakash ◽  
Sadia Parveen ◽  
Urvashi Mishra
Keyword(s):  
Virus Infection ◽  
Adolescent Girls ◽  
Rubella Virus ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Reproductive Age ◽  
Care Hospital ◽  
Rubella Infection ◽  
Area Of Interest ◽  
Rubella Virus Infection

Background: Rubella infection occurring during early pregnancy results in congenital rubella syndrome (CRS).  WHO estimates that worldwide more than 100,000 children are born with CRS each year and most of them are in the developing countries. For assessing population immunity against rubella, sero-surveys are generally recommended among adolescent girls and reproductive age female. In India, sero-surveys conducted by different authors have indicated that about 10-30% of adolescent females are susceptible to rubella infection. Adolescent girls are selected because they are at a critical stage of child bearing age and their immunity against Rubella infection is the particular area of interest. objective of this study was to estimate the sero-prevalence of unvaccinated adolescent girls susceptible to Rubella virus infection attending a tertiary care hospital of Patna and then accordingly counsel for vaccination.Methods: A total 150 adolescent girls in the age group of 10-19 years who had not received MMR vaccine were included in the study. Serum IgG antibody titer for rubella was estimated by the ELISA method.Results: A total 65.33% of the adolescent girls were found to be rubella seropositive and (34.67%) were seronegative. The urban adolescent girls had a higher seropositivity of 85.2% as compared to rural adolescent girls.Conclusions: The study indicates that a substantial number of adolescents (34.67%) are seronegative and hence susceptible to rubella infection.

scholarly journals Kinetics of IgG Antibodies in Previous Cases of Dengue Fever—A Longitudinal Serological Survey

2020 ◽  
Vol 17 (18) ◽  
pp. 6580
Author(s):  
Qilin Wu ◽  
Qinlong Jing ◽  
Xiujuan Wang ◽  
Lili Yang ◽  
Yilan Li ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Antibody Level ◽  
Southern China ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Related Factors ◽  
Factors Affecting ◽  
Serological Investigation ◽  
Significant Difference

Guangzhou is believed to be the most important epicenter of dengue outbreaks in southern China. In this study, a longitudinal serological investigation of previous cases of dengue fever in Guangzhou was conducted to explore the persistence of IgG antibodies and related factors affecting the changes of antibody level. We recruited 70 dengue virus type 1 (DENV-1) primary infection cases at two years post infection for serological investigation and conducted a second follow-up in the 5th year of prognosis. An enzyme-linked immunosorbent assay (ELISA) for DENV IgG antibody was examined in all study subjects. Potential factors associated with the concentration of serum total IgG antibody were determined by the generalized estimation equation (GEE). No significant difference in serum total IgG antibody positive rate between two follow-ups was observed (χ2 = 3.066, p = 0.080). However, there was a significant difference in the concentration of serum total IgG antibody between the two follow-ups (Z = 7.154, p < 0.001). The GEE showed that the antibody level in the five-year prognosis was mainly affected by the antibody level in the two-year prognosis (OR: 1.007, 95%CI: 1.005–1.009). In conclusion, the serum IgG antibodies of previous dengue fever cases can persist for a long time.

scholarly journals Association of the Arginase Ι with Bronchial Asthma

2019 ◽  
Vol 27 (2) ◽  
pp. 238-243 ◽  
Author(s):  
Ashti Mohammad Amin Said ◽  
Ahmed Abdulqader Al-Naqshbandi ◽  
Yasin Kareem Amin ◽  
Rebwar Jalal Ali
Keyword(s):  
Family History ◽  
Bronchial Asthma ◽  
Colorimetric Assay ◽  
Mean Value ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
Significant Difference ◽  
Asthma Patients ◽  
History Of ◽  
And Gender

Objective The aim of this study was to detect the arginase Ι (ARG I) enzyme in asthma patients, clarify its role, in addition to examining the relationship of this enzyme with bronchial asthma. Methods: Blood samples were collected from 100 patients from the Department of Medicine in Rizgary Hospital in Erbil City, in addition to intact 100 volunteers; the introduced questionnaire was filled out on the basis of type-induced asthma, duration of the disease, age of the patients, gender, family history, and allergy condition, Serum was separated to perform Enzyme-linked immunosorbent assay (ELISA) in Medical Research Center to examine the association of ARGΙ with bronchial asthma. By ARG activity we can measure the conversion of arginine to ornithine and urea. By using a quantitative colorimetric assay at 490 nm, employing a QuantiChrom arginase assay kit (Bioassay Systems). Results: Our results depicted the association between ARGΙ and bronchial asthma: based on their age, significant elevation of serum arginase level was observed in the patients with ≥81 years old, which mean value (100.16±19.77c), p-value (0.000); also the duration of asthma ≥20 years (82.48±38.81c) , p-value (0.01) were remarkably affected; this sign was found in those with types of induced asthma and with allergy condition.  But the non- significant difference in the frequency of abnormal serum arginase level was observed in those patients that have a family history of asthma disease and gender of the patients. This finding demonstrated a remarkable association of ARG Ι in the development of asthma at p ˂ 0.05.

scholarly journals EVALUATION OF METABOLIC SYNDROME IN HEALTHY YEMENI POPULATIONS

2020 ◽  
Author(s):  
Gamil Alrubaiee ◽  
Ali Alyahawi
Keyword(s):  
Metabolic Syndrome ◽  
Peer Review ◽  
Fasting Blood Glucose ◽  
Morbidity And Mortality ◽  
Cross Sectional ◽  
The Metabolic Syndrome ◽  
Total Prevalence ◽  
Cardiovascular Morbidity And Mortality ◽  
Significant Difference ◽  
E Mail

The metabolic syndrome is characterized by several cardiovascular risk factors and is associated with an increased incidence of diabetes, cardiovascular events and mortality. The prevalence of metabolic syndrome is increasing in epidemic proportions worldwide. The present study aimed to investigate the prevalence of MS and its components in healthy populations in Sana'a, Yemen. This study was a cross-sectional study conducted from February 2019 to April 2019. A total of 120 healthy populations (>=40 years old) were selected. The study protocol was approved by the institutional ethical committee and informed consent was obtained from all the enrolled study patients for their inclusion in the screening and participation in the research. In the present study, the diagnosis of metabolic syndrome based on the American Heart Association/National Heart, Lung, and Blood Institute(AHA/NHL BI) and to a joint statement from several large organizations. In the current study, the presence of more than or equal to any three of the above mentioned factors is required for the diagnosis of metabolic syndrome. The total prevalence of MS among the study subjects was 40.0% (P< 0.001) and 62.5%of them were within 40-49 years old. In the present study, there was not statically significant difference between the khat chewing and the metabolic syndrome. According the distribution of metabolic syndrome criteria among subjects with metabolic syndrome, the prevalence of fasting blood glucose (FBG) was the highest (85%). The prevalence of metabolic syndrome among healthy Yemeni populations was very high and it is associated with increased morbidity and mortality.  This emphasizes the need for more attention to investigate this condition to decreasing the prevalence of cardiovascular morbidity and mortality in these subjects. Peer Review History: UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Name: Dr. Hassan A.H. Al-Shamahy Affiliation: Sana'a University, Yemen E-mail: [email protected]   Name: Dr. Muhammad Shahzad Aslam Affiliation: Universiti Malaysia Perlis, Malaysia E-mail: [email protected] Comments of reviewer(s): Similar Articles: PREVALENCE OF HEPATITIS G VIRUS AMONG PATIENTS WITH CHRONIC LIVER DISEASE AND HEALTHY INDIVIDUALS, SANA'A CITY-YEMEN  

scholarly journals Association of Leptin Gene Polymorphisms with Rheumatoid Arthritis in a Chinese Population

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Sha-Sha Tao ◽  
Yi-Lin Dan ◽  
Guo-Cui Wu ◽  
Qin Zhang ◽  
Tian-Ping Zhang ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Chinese Population ◽  
Clinical Manifestations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Genotype Frequencies ◽  
Significant Difference ◽  
Plasma Leptin

Background. Recently, increasing studies have revealed that leptin is involved in the development of rheumatoid arthritis (RA). This study is aimed at exploring the association of leptin gene single nucleotide polymorphisms (SNPs) with susceptibility to RA in a Chinese population. Methods. We recruited 600 RA patients and 600 healthy controls from a Chinese population and analyzed their three leptin SNPs (rs10244329, rs2071045, and rs2167270) using the improved Multiplex Ligase Detection Reaction (iMLDR) assays. The associations of these SNPs with clinical manifestations of RA were also analyzed. Enzyme-linked immunosorbent assay (ELISA) was performed for plasma leptin determination. Results. No significant difference in either allele or genotype frequencies of these three SNPs between RA patients and healthy controls was observed (all P > 0.05 ). Association between the genotype effects of dominant, recessive models was also not found (all P > 0.05 ). No significant difference in plasma leptin levels was detected between RA patients and controls ( P > 0.05 ). Conclusion. Leptin gene (rs10244329, rs2071045, and rs2167270) polymorphisms are not associated with RA genetic susceptibility and its clinical features in the Chinese population.

scholarly journals Anti-S. typhi Vi IgG levels in children with and without typhoid vaccinations

2014 ◽  
Vol 54 (5) ◽  
pp. 284
Author(s):  
Sriandayani Sriandayani ◽  
Tonny H. Rampengan ◽  
Hesti Lestari ◽  
Novie Rampengan
Keyword(s):  
Typhoid Fever ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Igg Antibody ◽  
Protective Antibody ◽  
Significant Difference ◽  
Antibody Titers ◽  
The Mean ◽  
And Gender ◽  
Antibody Levels

Background Typhoid fever is endemic to Indonesia, with an annual incidence of 13/10,000 people. Vaccination has been shown to be an effective method to prevent typhoid fever. Of several vaccine types, the polysaccharide Vi vaccine is the most commonly used typhoid vaccine in developing countries. Results of previous studies remain inconclusive on the necessity of revaccination every 3 years.Objective To compare the mean serum anrioody titers of anti-S. typhi Vi IgG and the proportion of children with protective antibody levels between children with and without typhoid Vi vaccination.Methods We conducted a cross-secrional study at Tuminring District, 11anado from June to September 2012. Data was analyzed using independent T-test and Fisher's test. Serum anti-S. typhi Vi IgG levels were measured by enzyme-linked immunosorbent assay (ELISA) method.Results Seventy-six subjects were divided into two groups: 38 children who had received the typhoid Vi vaccination more than 3 years prior to this study and 38 children who never had typhoid vaccinations as a control group. No statistically significant difference in age and gender was found between the two groups. The mean serum anti-Vi IgG level was 0.55 ug/mL (SD 0.58; 95%CI 0.36 to 0.74) in the vaccinated group, significantly higher than that of the control group [0.31 ug/mL (SD 0.12); 950/£1 0.17 to 0.44; P􀂥0.0381. The proportion of children with protective antiNi antioody level was higher in the vaccinated group (23.7%) than in the control group  (10.5%), howevet; this difference was not statistically significant (P=0.128).Conclusion The mean serum anti-S. typhi Vi IgG antibody level in children who had been vaccinated more than 3 years prior to the study is higher than in children who had never received typhoid vaccinations. Nevertheless, the mean antibody titers are generally non-protective in ooth groups. Also, the proportion of children with protective antibody levels is not significantly different between the two groups.

scholarly journals SAT0057 PREDICTING INADEQUATE RESPONSE TO JAK INHIBITORS BY CLUSTER ANALYSIS IN PATIENTS WITH RHEUMATOID ARTHRITIS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 960.1-961
Author(s):  
M. Sugawara ◽  
Y. Fujieda ◽  
A. Noguchi ◽  
S. Tanimura ◽  
Y. Shimizu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Cluster Analysis ◽  
Bone Erosion ◽  
Japanese Patients ◽  
User Group ◽  
Biologic Dmards ◽  
Chugai Pharmaceutical ◽  
Significant Difference ◽  
History Of ◽  
Group B

Background:Oral Janus kinase inhibitors (JAKi) have dramatically altered outcomes in patients with rheumatoid arthritis (RA). However, there remains some proportion of patients who respond to inadequately JAKi treatment (JAKi-IR) [1,2]. The characteristics in RA patients associated with JAKi-IR have not been fully demonstrated.Objectives:To clarify the characteristics of JAKi-IR in patients with RA by cluster analysis.Methods:This retrospective study comprised 120 RA patients who were treated with JAKi (Tofacitinib or Baricitinib) between July 2013 and September 2019 in five facilities. The disease status at the baseline, at 12 weeks after JAKi treatment and at the time point of withdrawing JAKi was assessed using the Disease Activity Score (DAS28) and the American College of Rheumatology (ACR) response criteria. JAKi-IR was defined as follows, primary non-response at 12 weeks after JAKi treatment: withdrawal of JAKi with ACR20 non-response or non-improvement in DAS28-CRP (ΔDAS28-CRP<1.2 from baseline), secondary non-response: withdrawal of JAKi without clinical remission after 12 weeks. Hierarchical cluster analysis was performed with the following variables: gender, age, disease duration, bone erosion, ACR functional classification (Class ≥3), comcomitant rheumatoid arthritis related interstitial lung disease (RA-ILD) or other autoimmune disease (AID), anti-citrullinated protein antibody (ACPA) positivity, rheumatoid factor (RF) at baseline, use/dose of methotrexate (MTX) and prednisolone (PSL), serum ESR/CRP, tender/swollen joint counts (TJC/SJC), visual analog scale by patients (VAS-Pt), and prior of biologic DMARDs.Results:The 120 enrolled patients were classified into 4 groups by cluster analysis(Figure1), The characteristics of each group are as follows, Group A(n=21): female + bone erosion + RF/ACPA positive + AID + MTX non-user, Group B(n=36): male + older age + RA-ILD + RF/ACPA positive + MTX non-user, Group C(n=35): RF/ACPA positive + absence of RA-ILD + MTX user, Group D (n=28): seronegative + MTX user + absence of RA-ILD + history of biologic DMARDs failure. The rate of JAKi-IR was A:9%, B:8%, C:20%, D:32%, and the significant difference between Group B and D was identified (p=0.02). In multiple comparison of 4 groups, no significant difference was identified (p=0.06) (Figure2).Conclusion:JAKi-IR would be more likely to be seronegative, MTX use, absence of RA-ILD and history of biologic DMARDs failure. Cluster analysis is an exploratory tool that aids in the analysis of huge amount of data.References:[1] Takeuchi T, Yamanaka H, Yamaoka K, Arai S, Toyoizumi S, DeMasi R, et al. Efficacy and safety of tofacitinib in Japanese patients with rheumatoid arthritis by background methotrexate dose: A post hoc analysis of clinical trial data. Mod Rheumatol. 2019;29(5):756-66.[2] Tanaka Y, Atsumi T, Amano K, Harigai M, Ishii T, Kawaguchi O, et al. Efficacy and safety of baricitinib in Japanese patients with rheumatoid arthritis: Subgroup analyses of four multinational phase 3 randomized trials. Mod Rheumatol. 2018;28(4):583-91.Disclosure of Interests:Masanari Sugawara: None declared, Yuichiro Fujieda: None declared, Atsushi Noguchi: None declared, Shun Tanimura: None declared, Yuka Shimizu: None declared, Ikuma Nakagawa: None declared, Michihito Kono: None declared, Masaru Kato: None declared, Kenji Oku: None declared, Tatsuya Atsumi Grant/research support from: Eli Lily Japan K.K., Alexion Pharmaceuticals, Inc., Bristol-Myers Squibb Co., AbbVie Inc., Daiichi Sankyo Co., Ltd., Pfizer Inc., Chugai Pharmaceutical Co., Ltd., Mitsubishi Tanabe Pharma Co., Astellas Pharma Inc., Consultant of: Gilead Sciences, Inc., Eli Lilly Japan K.K., UCB Japan Co. Ltd., AbbVie Inc., Daiichi Sankyo Co., Ltd., Pfizer Inc., Chugai Pharmaceutical Co., Ltd., Speakers bureau: Eli Lilly Japan K.K., UCB Japan Co. Ltd., Bristol-Myers Squibb Co., AbbVie Inc., Eisai Co. Ltd., Otsuka Pharmaceutical Co., Ltd., Daiichi Sankyo Co., Ltd., Pfizer Inc., Chugai Pharmaceutical Co., Ltd., Mitsubishi Tanabe Pharma Co., Takeda Pharmaceutical Co., Ltd., Astellas Pharma Inc.

scholarly journals Seroprevalence of Subclinical HEV Infection in Healthy Pregnant Urban Dwellers of Bangladesh: Identification of Possible Risk Factors

2018 ◽  
Vol 8 (2) ◽  
pp. 85-89
Author(s):  
Gazi Zakia Sultana ◽  
Md Moniruzzaman ◽  
Tania Mannan ◽  
Rosy Sultana
Keyword(s):  
Risk Factors ◽  
Socioeconomic Status ◽  
Pregnant Women ◽  
Low Socioeconomic Status ◽  
Economic Status ◽  
Education Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Cross Sectional ◽  
History Of

Background: Hepatitis caused by hepatitis E Virus (HEV) is not uncommon in developing countries. It is usually a self-limiting conferring immunity against subsequent infection. However, HEV infection during pregnancy results in varying degree of morbidity, often fatal. The present study was designed to find out the seroprevalence of subclinical HEV infection during pregnancy at different trimesters without history of hepatitis.Materials and Methods: A total 255 asymptomatic healthy pregnant women of three trimesters (85×3=255) with no history of jaundice were included in this cross-sectional study. The subjects were sub-grouped according to socioeconomic status and education level. HEV IgG antibody in serum was determined by enzyme linked immunosorbent assay (ELISA). Results were expressed as number (percent). Chi-square, Odds Ratio and 95% CI were calculated as applicable. Data analyses were carried out using statistical package for social science for Windows Version 15.0. A p<0.05 was taken as level of significance.Results: Seropositivity for HEV IgG was 38% (96/255) in pregnant women; the higher percentages were recorded in the 2nd and 3rd trimesters − 41% and 46% respectively. The seropositivity of HEV IgG was significantly high in pregnant women with low education level ((p=0.001; OR=2.70, 95% CI=1.602−4.575) and low socioeconomic status (OR=7.54, 95% CI=4.118−13.029) having monthly income below 27,000 taka (p=0.001).Conclusion: Data concluded that seroprevalence of anti-HEV IgG is higher at third trimester in pregnant women in Bangladesh where low socio-economic status and less education level were identified as possible risk factors. Appropriate measures may diminish the possible exposure to infection and reduce maternal mortality.J Enam Med Col 2018; 8(2): 85-89

Effects of Moxibustion on the Enhancement of Serum Antibody in Rabbit againstStaphylococcus aureus

1998 ◽  
Vol 26 (01) ◽  
pp. 29-37 ◽  
Author(s):  
Hitoshi Yamashita ◽  
Yoshitoshi Ichiman ◽  
Masami Takahashi ◽  
Kazushi Nishijo
Keyword(s):  
Staphylococcus Aureus ◽  
Immunosorbent Assay ◽  
Serum Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Protective Activity ◽  
Igg Antibody ◽  
Moxibustion Group ◽  
Significant Difference ◽  
Elisa Inhibition

The titer and activity of antibody in rabbits immunized with heat-killed vaccine were assessed with and without moxibustion treatment. Enzyme-linked immunosorbent assay (ELISA) was applied for the detection of immunoglobulins to Smith strain of Staphylococcus aureus. Positive IgM titer of more than 0.4 were observed with this assay against the moxibustion group (P < 0.05). The titer of IgG antibody also increased; however, there was no significant difference between the moxibustion group and the control group. The ELISA inhibition test showed significantly higher protective activity of the sera in the moxibustion group at the 9th week after the first immunization (P < 0.05).

scholarly journals Pulmonary involvement in rheumatoid arthritis: evaluation by radiography and spirometry

2015 ◽  
Vol 41 (4) ◽  
pp. 331-342 ◽  
Author(s):  
Alexandre Melo Kawassaki ◽  
Daniel Antunes Silva Pereira ◽  
Fernando Uliana Kay ◽  
Ieda Maria Magalhães Laurindo ◽  
Carlos Roberto Ribeiro Carvalho ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Smoking Status ◽  
Pulmonary Involvement ◽  
Diagnostic Methods ◽  
Test Results ◽  
Cross Sectional ◽  
X Ray ◽  
Significant Difference ◽  
History Of ◽  
Chest X Ray

AbstractObjective: To determine whether simple diagnostic methods can yield relevant disease information in patients with rheumatoid arthritis (RA).Methods: Patients with RA were randomly selected for inclusion in a cross-sectional study involving clinical evaluation of pulmonary function, including pulse oximetry (determination of SpO2, at rest), chest X-ray, and spirometry.Results: A total of 246 RA patients underwent complete assessments. Half of the patients in our sample reported a history of smoking. Spirometry was abnormal in 30% of the patients; the chest X-ray was abnormal in 45%; and the SpO2 was abnormal in 13%. Normal chest X-ray, spirometry, and SpO2 were observed simultaneously in only 41% of the RA patients. A history of smoking was associated with abnormal spirometry findings, including evidence of obstructive or restrictive lung disease, and with abnormal chest X-ray findings, as well as with an interstitial pattern on the chest X-ray. Comparing the patients in whom all test results were normal (n = 101) with those in whom abnormal test results were obtained (n = 145), we found a statistically significant difference between the two groups, in terms of age and smoking status. Notably, there were signs of airway disease in nearly half of the patients with minimal or no history of tobacco smoke exposure.Conclusions: Pulmonary involvement in RA can be identified through the use of a combination of diagnostic methods that are simple, safe, and inexpensive. Our results lead us to suggest that RA patients with signs of lung involvement should be screened for lung abnormalities, even if presenting with no respiratory symptoms.

Sign in / Sign up

Export Citation Format

Share Document