Enkapsulasi bakteri asam laktat dari tempoyak asal Jambi: Pengaruh konsentrasi alginat [Encapsulation of lactic acid bacteria isolated from fermented durian (tempoyak) in Jambi: Influence of alginate concentration]

This study aimed to investigate the characteristics and the viability of encapsulated lactic acid bacteria (LAB) from fermented durian from Jambi using alginate concentration as wall material. The encapsulation method used the emulsification technique where LAB in alginate capsules was formed by the formation of water in oil emulsion. This study used a completely randomized design with various alginate concentrations as the treatments with concentrations: 1%, 3%, 5%, 7%, and 9%, respectively. The result showed that alginate concentration on LAB microcapsules was very significant in yield, pH, and heat resistance. All treatment concentrations provided high LAB viability, so alginate is effective for wall material LAB encapsulation with emulsification techniques. The best LAB microcapsule was 7% of alginate concentration with value of yield 5,60 %, viability of LAB log 12,43 CFU/g, pH 5,22, heat resistant on 50ºC (log 12, 46 CFU/g), 60ºC (log 12,45 CFU/g), 70ºC (log 12,35 CFU/g).

Download Full-text

Pengaruh pH terhadap Produksi Antibakteri oleh Bakteri Asam Laktat dari Usus Itik

Jurnal Penelitian Pertanian Terapan ◽

10.25181/jppt.v15i3.135 ◽

2017 ◽

Vol 15 (3) ◽

Author(s):

Rudy Sutrisna ◽

Christina Nugroho Ekowati ◽

Edelina Sinaga Sinaga

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Analysis Of Variance ◽

Antibacterial Effect ◽

Inhibition Zone ◽

Bacterial Isolates ◽

Clear Zone ◽

Effect Of Ph ◽

Randomized Design ◽

Completely Randomized Design

This study aimed to determine the effect of pH on the production of antibacterial by lactic acid bacteria. This study uses a completely randomized design factorial 3X 5 (3 X 5 treatment of bacterial isolates pH) with three replications. The first factor consisted of pH 4, pH 5, pH 6, pH 7, pH 8, and the second factor that isolates B2, B7, B8, whereas the parameters consists of a clear zone. The amount of production is determined by the size of the diameter of antibacterial inhibition zone against Salmonella sp. Differences in the production of antibacterial determined based on the results of analysis of variance. The treatment significantly further test BNT at 5% level. The results showed that the effect of media pH test based on the potential for the production of antibacterial effect. The diameter of the largest clear zone produced by B7 isolates at pH 6 was 17.2 mm, 17.1 mm B8 and B2 at pH 7 at 16.7 mm. The smallest diameter of the clear zone produced by B8 at pH 4 was 15.1 mm, B2 at pH 6 there is a clear zone of 12.7 mm and isolate B7 at pH 5 of 11.5 mm Keywords: Antibacterial, Lactic Acid Bacteria, pH, Salmonella sp, Clear zone

Download Full-text

Potential and characteristics of dadiah processed into products-like nuggets with addition of cinnamon bark flour (Cinnamomum burmanni)

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/888/1/012036 ◽

2021 ◽

Vol 888 (1) ◽

pp. 012036

Author(s):

A Sandra ◽

E Purwati ◽

A Hasibuan

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Experimental Method ◽

Buffalo Milk ◽

Natural Fermentation ◽

Cinnamon Bark ◽

Cholesterol Levels ◽

Randomized Design ◽

West Sumatra ◽

Completely Randomized Design

Abstract Dadiah is a natural fermentation of buffalo milk from West Sumatra, which has a distinctive smell and flavor. This causes the dadiah to be less liked, so it is necessary to diversify the processed of dadiah. This study aimed to determine the effect of adding cinnamon flour (Cinnamomum burmanni) in the processing of nugget-like products on antioxidants, cholesterol, the total colony of lactic acid bacteria and organoleptic values. This research used 2000 grams of dadiah and cinnamon bark flour (15 g). The variables observed were antioxidants, cholesterol, the total colony of lactic acid bacteria and organoleptic values. The method used in this study was an experimental method using a Completely Randomized Design (CRD) with 4 treatments and 5 replications. The treatments in this study were the addition of cinnamon flour namely A (0%), B (0.5%), C (1%), D (1.5%). The results of this study indicated that the addition of cinnamon flour to nugget-like products had a significant effect (P<0.05) increasing antioxidant levels and decreasing cholesterol levels, not significantly different (P> 0.05) on the total colony test of lactic acid bacteria and the organoleptic test of taste, texture and flavor. The addition of cinnamon flour to nugget-like products in treatment D (1.5%) gave the best results with antioxidant levels of 73.24%, cholesterol levels of 20.32 mg/dl, the total colony of lactic acid bacteria 1.48x107 CFU / ml and taste organoleptic value 3.44, texture 3.68 and flavor 3.80.

Download Full-text

The Characteristics and Potential of Lactic Acid Bacteria as Probiotics in Silage Made from Hymenachne acutigluma and Neptunia oleracea lour

E3S Web of Conferences ◽

10.1051/e3sconf/20186801017 ◽

2018 ◽

Vol 68 ◽

pp. 01017

Author(s):

Sofia Sandi ◽

Fitra Yosi ◽

Meisji Liana Sari ◽

Nuni Gofar

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Plantarum ◽

Gram Positive ◽

Gram Staining ◽

Randomized Design ◽

Different Temperatures ◽

Completely Randomized Design ◽

H2s Production

This study aims to determine the characteristics of lactic acid bacteria (LAB) obtained from the silage made from Hymenachne acutigluma and Neptunia oleracea Lour to be used as probiotics. This study used a completely randomized design (CRD), which consists of 3 treatments and 5 replications. The treatment consisted of P1 (silage made from Hymenachne acutigluma), P2 (silage made from 50% of Hymenachne acutigluma and 50% of Neptunia oleracea lour), and P3 (silage made from Neptunia oleracea lour). The variables measured were LAB characteristics including shape, gram staining, catalase, endospore, motility, growth at different temperatures, TSIA, MR, VP, and identification using API Kit. The data were analyzed descriptively. The results showed that there were 9 selected isolates. All isolates are rod-shaped, gram-positive, catalase, endospore, and negative motility, growth at 15 °C negative and at 45 °C positive. TSIA slant and butt test show yellow and negative colors on gas and H2S production, while MR-VP is red and yellow. The conclusions of this study were all isolates belonging to the Lactobacillus plantarum strain with a similarity level of 87.3-99.9 %.

Download Full-text

POPULASI BAKTERI ASAM LAKTAT PADA BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTASI LIMBAH PERTANIAN DENGAN SUPLEMEN ENCENG GONDOK DAN PROBIOTIK

Scripta Biologica ◽

10.20884/1.sb.2014.1.1.32 ◽

2014 ◽

Vol 1 (1) ◽

pp. 87

Author(s):

Andhika Agus Setyawan ◽

Sukanto Sukanto ◽

Endang Widyastuti

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Water Hyacinth ◽

Agricultural Waste ◽

Randomized Design ◽

Fermented Feed ◽

Independent Variable ◽

Completely Randomized Design ◽

Chemical Conditions ◽

Physical And Chemical

The aim of this study were to determine the effect of fermented agricultural wastes feeding with supplement of water hyacinth and MEP+ probiotic towards the increasing population of intestinal Lactid Acid Bacteria (LAB) in Tilapia and to know the highest population of lactic acid bacteria after fermented agricultural waste feeding with water hyacinth supplements and MEP+ probiotic. Independent variable in this study was fermented feed with the addition of different water hyacinth and MEP+ probiotics. The experimental study was continued using completely randomized design (CRD). The dependent variable in this study was the number density of Lactobacillus bacteria in the gut of Tilapia. The main parameters measured were the number of LAB. Proponent parameters were namely Tilapia weight gain, physical and chemical conditions of the water. The results showed that feeding fermentative effected in increasing the population of lactic acid bacteria in the gut of Tilapia. Fermentative feed supplement agricultural waste with water hyacinth as much as 10% and MEP+ probiotic was the best treatment with LAB population density in Tilapia intestine with the average of 5.85 x 106 CFU /g.

Download Full-text

Perubahan Karakteristik Kimia dan Mikrobiologis Jruek Drien (Durian Fermentasi Khas Aceh) selama Fermentasi

Jurnal Teknologi dan Industri Pertanian Indonesia ◽

10.17969/jtipi.v10i2.6123 ◽

2018 ◽

Vol 10 (2) ◽

pp. 1-5

Author(s):

Murna Muzaifa ◽

Ryan Moulana ◽

Yuliani Aisyah ◽

Zainuddin Zainuddin ◽

Faidha Rahmi

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Water Content ◽

Sugar Content ◽

Fermented Food ◽

Total Acid ◽

Total Sugars ◽

Randomized Design ◽

Microbiological Characteristics ◽

Completely Randomized Design

Jruek drien is one of traditional fermented food of Aceh. This product made from flash of durio that fermented without starter addition. Explorative research has been conducted to examine the change of chemical and microbiological characteristics of jruek drien during fermentation. This study used completely randomized design with single factor (length of fermentation: 1,3,5 and 7 days). The parameters analyzed were water content, pH, total acid, total sugars, total lactic acid bacteria and yeast. The results showed that water content and acid total were increased while pH and sugar content were decreased. The highest total of lactic acid bacteria was found on day 3. Yeast detected only on early fermentation and no detected on 7th day.

Download Full-text

PEMBUATAN YOGHURT SINBIOTIK KACANG MERAH (Phaseolus vulgaris L.) DENGAN PENGGUNAAN BAKTERI ASAM LAKTAT DENGAN PENAMBAHAN PREBIOTIK

JURNAL BIOINDUSTRI ◽

10.31326/jbio.v2i2.655 ◽

2020 ◽

Vol 2 (2) ◽

pp. 476-486

Author(s):

Agatha Sonya Sekarningrum ◽

◽

Seveline Seveline ◽

Keyword(s):

Lactic Acid ◽

Phaseolus Vulgaris ◽

Lactic Acid Bacteria ◽

Phaseolus Vulgaris L ◽

Randomized Design ◽

Probiotic Product ◽

Banana Flour ◽

Completely Randomized Design ◽

The Right

Manufacture of probiotic product that has been done a lot is manufacture of yoghurt. In the process, addition of red beans and prebiotic is done to add value to the product. Addition of Lactic Acid Bacteria and inulin in the form of banana flour with different amounts is done to find the right synbiotic yogurt formulation. This research was conducted to test the quality of product physically, chemically, microbiology and organoleptics. This research was conducted using a Completely Randomized Design with the first factor being the BAL levels added and the second factor being the levels of inulin added. Based on the tests, the results obtained that the formulation using L. plantarum as much as 1% and 2% commercial inulin is the right formulation with results that are in accordance with SNI 2891: 1992 standards. The total value of LAB resulting from the formulation is 3.24 x 106 cfu/ml with a total non-fat solid of 11.16%.

Download Full-text

KUALITAS FISIKOKIMIA DAN MIKROBIA YOGHURT SINBIOTIK YANG DIBERI PATI TERMODIFIKASI UMBI UWI UNGU (Dioscorea alata) DENGAN LEVEL BERBEDA

ZOOTEC ◽

10.35792/zot.40.1.2020.26922 ◽

2019 ◽

Vol 40 (1) ◽

pp. 124

Author(s):

Agnes C. Korengkeng ◽

A. Yelnetty ◽

Rahmawati Hadju ◽

M. Tamasoleng

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Ph Value ◽

Modified Starch ◽

Dioscorea Alata ◽

Microbial Quality ◽

Randomized Design ◽

Completely Randomized Design

THE PHYSICOCHEMICAL AND MICROBIAL QUALITY OF SINBIOTIC YOGHURT GIVEN DIFFERENT LEVEL OF MODIFIED STARCH OF PURPLE UWI BULB (Dioscorea alata). An experiment was done in order to determine the level of modified starch of purple uwi bulb (Dioscorea alata) which best for syneresis, pH and total lactic acid bacteria of synbiotic yoghurt. Completely Randomized Design (CRD) was used in this experiment with 6 treatments as follows P0 = yoghurt without modified starch purple uwi bulb, P1 = yoghurt + 0,1% modified starch purple uwi bulb, P2 = yoghurt + 0,2% modified starch purple uwi bulb, P3 = yoghurt + 0,3% modified starch purple uwi bulb, P4 = yoghurt + 0,4% modified starch purple uwi bulb dan P5 = yoghurt + 0,5 modified starch purple uwi bulb; and three repetitions each treatment. Measurements taken were syneresis, pH value, total of acid lactate bacteria. Data obtained were analyzed using ANOVA and Tuckey’s test was used to inspect treatment differences among group means. It found in this research that application of modified starch of purple uwi bulb in symbiotic yoghurt gave a significant effect (P<0,01) on pH value and total lactic acid bacteria but not on syneresis. However, the syneresis values tendency decreased as the level of modified starch of purple uwi bulb increased. The pH of symbiotic yoghurts given modified starch were ranged between 5.0 – 5.4 and the lowest pH was found in yoghurt with 0.3% modified starch added, meanwhile the total lactic acid bacteria of symbiotic yoghurts given modified starch were ranged between log 10-78.65 – log10-7 8.87 and the highest total lactic acid bacteria was found in yoghurt with 0.2% modified starch added. It concluded in this research that the best quality of symbiotic yoghurt was in yoghurt with addition of modified starch of purple uwi bulb 0.3%. Keywords: Sinbiotic Yoghurt, Modified Starch of Ubi Purple Tuber, Sineresis, pH value, BAL Total

Download Full-text

Analisa Keasaman dan Total Bakteri Asam Laktat Yogurt Akibat Bahan Baku dan Persentase Lactobacillus casei yang Berbeda

Jurnal Agripet ◽

10.17969/agripet.v13i2.817 ◽

2013 ◽

Vol 13 (2) ◽

pp. 31-35

Author(s):

Yusdar Zakaria ◽

Yurliasni Yurliasni ◽

Mira Delima ◽

Ely Diana

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Casei ◽

Ph Value ◽

Acid Value ◽

Fresh Milk ◽

Randomized Design ◽

Significant Difference ◽

Two Factors ◽

Completely Randomized Design

Analyze of acidity and amount of lactic acid bacteria of yogurt made from different milk types and lactobacillus casei percentageABSTRACT. The objective of this experiment is to determine the effect of milk types and percentages of Lactobacillus casei as a starter to the amount of Lactic Acid Bacteria in yogurt and its pH value and Lactic Acid value. Factorial Completely Randomized Design with two factors and three replications was applied. The A factor was the milk types that were a1 = Powder Milk, a2 = Fresh Milk and a3 = UHT milk. The B factor was the percentages of Lactobacillus casei (b1 = 5% and b2 = 10%). Parameters observed were the level of lactic acid, the pH value of yogurt and the amount of lactic acid bacteria. The result showed significantly difference (P0.01) from the use of milk types and Lactobacillus casei percentages on the lactic acid level, and there are no interaction between factors. There was also significant different (P0.01) showed from pH value and an interaction between both factors was presented. In addition there was significant difference (P0.05) showed by the amount of lactic acid bacteria, and an interaction between both factors was also existed. The research concluded that the higher the percentage of starter, the higher the level of lactic acid and the amount of lactic acid bacteria of yogurt that made from all milk types. On the contrary, the higher the percentage of starter, the lower the pH value of yogurt.

Download Full-text

PENGARUH NISBAH C/N CAMPURAN LIMBAH MILK TEA DAN MOLASSES TERHADAP JUMLAH BAKTERI ASAM LAKTAT, pH, PERUBAHAN FISIK WARNA, DAN AROMA PADA PROBIOTIK

Jurnal Teknologi Hasil Peternakan ◽

10.24198/jthp.v1i2.27563 ◽

2020 ◽

Vol 1 (2) ◽

pp. 47

Author(s):

Abdul Karim ◽

Deden Zamzam Badruzzaman ◽

Wowon Juanda ◽

Yuli Astuti Hidayati

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Analysis Of Variance ◽

Research Method ◽

Tukey Test ◽

Randomized Design ◽

Tea Waste ◽

Completely Randomized Design ◽

Physical Changes

The objective of the research is to identify the effect of various C/N ratio mixed of milk tea waste and molasses on amount of lactic acid bacteria, pH, physical changes color and aroma in probiotics. The material used in this research are milk tea waste and molasses, fermented in anaerobic. The research method uses a Completely Randomized Design (CRD) by 3 treatments, which are P1 = C/N ratio 25, P2 = C/N ratio 30, P3 = C/N ratio35, with 6 repetitions. The measured parameters are amount of lactic acid bacteria, the pH and physical changes color and aroma. The data were analyzed by Analysis of Variance (ANOVA) method based of Tukey Test. The results of this research showed that there is a significant effect (P < 0,05) between a various of C/N ratio on amount of lactic acid bacteria, pH levels and physical changes color and aroma in the results of fermentation (probiotics). The best respond is on the C/N ratio 30, so it can produced high amount of lactic acid bacteria with an average of 2,06 x 109 CFU/mL, an average pH of 3,75 and physical changes in color and aroma.

Download Full-text

The Effect of Storage Time on the Total Lactic Acid Bacteria and Presence of Gram Positive and Negative Bacteria in Calf Starter Pellet Added with Fermented Cabbage Waste

Buletin Peternakan ◽

10.21059/buletinpeternak.v43i1.38478 ◽

2019 ◽

Vol 43 (1) ◽

Author(s):

Sri Mukodiningsih ◽

Retno Iswarin Pujaningsih ◽

Resniati Resniati ◽

Andriyani Astuti

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Storage Time ◽

Microbiological Quality ◽

Gram Positive ◽

Gram Positive Bacteria ◽

Randomized Design ◽

Completely Randomized Design ◽

Materials Used

The aim of this research was to examine the microbiological quality of calf starter pellet added with fermented cabbage waste after stored for 0, 4, and 6 weeks. The materials used in this research consisted of cornmeal, rice bran, soybean meal, molasses, mineral mix, and fermented cabbage waste. This research used Completely Randomized Design with 4 treatments and 3 replications. The mixed calf starter pellet consisted of 100% calf starter and 6% of fermented cabbage waste, and then stored for 0 weeks (P0), 2 weeks (P1), 4 weeks (P2) and 6 weeks (P3). The observed microbial qualities were the total lactic acid bacteria and the presence of gram positive and negative bacteria in the mixed calf starters. The total lactic acid bacteria were analyzed descriptively, while the presence of gram bacteria was analyzed with analysis of variance followed with Duncan’s test. The result of this research showed that an increase in storage time would result in lower lactic acid bacteria population, while the gram-positive and negative bacteria was not significantly affected. The research concluded that 6-weeks stored calf starter pellet added with 6% of fermented cabbage waste could maintain its lactic acid and gram-positive bacteria population, while also reducing its gram-negative bacteria population as well.

Download Full-text