Estimation of the Antibiotic Activity against Pseudomonas sppIsolated from Ear Infection

2021 ◽  
Vol 53 (03) ◽  
pp. 227-231
Author(s):  
Ehsan F Hussein ◽  
Keyword(s):  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Age Groups ◽  
Biochemical Tests ◽  
Klebsiella Species ◽  
Ear Infection ◽  
Pseudomonas Spp ◽  
Antibiotic Susceptibility Patterns ◽  
Vitek 2

Background: An ear infection, can be classified into otitis externs and otitis media, this affects all age groups especially infants and young. This infection associate with pathogenic microorganism type, frequent antibiotic uses, health care and age. The most common pathogenic bacteria of the ear infection are Pseudomonas spp. Antibiotic resistance represents a serious threat to the health of humans. Methods: 48 ear swabs were collected through the use of wooden sticks in a sterile container for the identification of Pseudomonas, Serratiaand Klebsiella species by VITEK 2 system and biochemical tests. The antibiotic susceptibility against these bacterial species was detected through the method of standard disk diffusion on the Moller Hinton agar. Results: Among ear swabs, the positive growth percentage of the pathogenic gram-negative bacteria was 29.166%, and the percentage of Pseudomonas spp. was 57.142%. Males were found to be more susceptible than females with an infection percentage of 57.142%. Conclusion: The antibiotic susceptibility patterns show the azithromycin, gentamycin, piperacillin, ceftriaxone, cefepime, imipenem, ceftriazon, and gentamycin have activity against Pseudomonas spp.

scholarly journals Enumeration of the Antimicrobial Susceptibility Patterns of Different Bacterial Isolates from ENT Patients with Ear Infections

2020 ◽  
pp. 68-73
Keyword(s):  
Developing Countries ◽  
Culture Media ◽  
Bacterial Species ◽  
Positive Culture ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Ear Infection ◽  
Pseudomonas Spp ◽  
Multiple Drugs ◽  
Klebsiella Spp

Children are more susceptible to ear infection than adults worldwide, especially in developing countries. In Bangladesh like other developing countries antibiotics is a common choice of treatment in an ear infection. This study was sought to determine causative agents both in right and left ear infection and their antibacterial susceptibility pattern from patients with an ear infection. Specimens of ear discharge were collected aseptically using a sterile swab and cultured on MacConkey’s agar, blood agar, and chocolate agar. After inoculating on appropriate culture media bacterial isolates were identified by their colony morphology, culture characteristics, Gram reaction, and biochemical tests. In this study, a total of 70 positive cases of patients were included. Among these 27 (38.6%) were male, and 43(61.4%) were female. The prevalence of ear infection was significantly high in females (61.4%) than males (38.6%). The predominant bacterial isolates from positive culture were Staphylococcus aureus 43(61.4%), followed by Pseudomonas spp., 13 (18.6%), Streptococcus spp., 8(11.4%), Proteus spp., 5(7.2%), and Klebsiella spp., 1(1.4%). The antibacterial agent like Amikacin and Gentamicin showed a high level of antibacterial effect on all identified bacterial isolates. On the other hand, 98.6% of isolates were found highly resistant to Co-Trimoxazole and Flucloxacillin. Moreover, Streptococcus spp., Proteus spp., Klebsiella spp., and Pseudomonas spp., were highly resistant to multiple drugs (more than 4). A high degree of antibiotic resistance was observed among most of the drugs used in this study. However, Amikacin and Gentamicin were highly effective against the isolated bacterial species. Therefore, culture and susceptibility tests are vital for the appropriate treatment of ear infection.

scholarly journals Molecular Characterization of Bacteria, Detection of Enterotoxin Genes, and Screening of Antibiotic Susceptibility Patterns in Traditionally Processed Meat Products of Sikkim, India

2021 ◽  
Vol 11 ◽  
Author(s):  
Meera Ongmu Bhutia ◽  
Namrata Thapa ◽  
Jyoti Prakash Tamang
Keyword(s):  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Meat Products ◽  
Resistance Pattern ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Meat ◽  
Rrna Gene ◽  
Antibiotic Susceptibility Patterns ◽  
Susceptibility Patterns

The lesser-known traditionally processed meat products such as beef kargyong, pork kargyong, satchu, and khyopeh are popular food items in the Himalayan state of Sikkim in India. The present study aimed to assess the microbiological safety of traditional meat products by identifying the potential spoilage or pathogenic bacteria, detecting the enterotoxins, and screening the antibiotic susceptibility patterns. The pH and moisture contents of the meat products varied from 5.3 to 5.9 and from 1.5 to 18%, respectively. The microbial loads of aerobic bacteria were 105 to 107 cfu/g, Staphylococcus 103 to 106 cfu/g, Bacillus 104 to 106 cfu/g, and total coliform 102 to 107 cfu/g, respectively. Based on 16S rRNA gene sequencing, the bacterial species isolated from traditionally processed meat products were Staphylococcus piscifermentans, Citrobacter freundii, Enterococcus faecalis, Salmonella enterica, Staphylococcus aureus, Citrobacter werkmanii, Klebsiella pneumoniae, Macrococcus caseolyticus, Klebsiella aerogenes, Staphylococcus saprophyticus, Pseudocitrobacter anthropi, Citrobacter europaeus, Shigella sonnei, Escherichia fergusonii, Klebsiella grimontii, Burkholderia cepacia, and Bacillus cereus. The enzyme-linked immunosorbent assay (ELISA) tests detected Salmonella spp. and enterotoxins produced by B. cereus well as Staphylococcus in a few tested samples. However, the PCR method did not detect the virulence genes of B. cereus and Salmonella in the isolates. Virulence gene (sea) was detected in S. piscifermentans BSLST44 and S. piscifermentans BULST54 isolated from beef kargyong and in S. aureus PSST53 isolated from pork kargyong. No enterotoxins were detected in khyopeh samples. The antibiotic sensitivity test showed that all bacterial strains were susceptible toward gentamicin, cotrimoxazole, norfloxacin, and trimethoprim. Gram-positive bacteria showed 100% sensitivity against clindamycin and erythromycin; however, 50% of the resistance pattern was observed against oxacillin followed by penicillin (33%) and ampicillin (27%).

scholarly journals Antibiotic Susceptibility Profile of Bacteria Isolated from Kenyan Bank Notes Circulating in Nyeri Town

2019 ◽  
pp. 1-12 ◽  
Author(s):  
S. G. Muguongo ◽  
A. K. Nyamache ◽  
J. M. Maingi
Keyword(s):  
Antibiotic Susceptibility ◽  
Burkholderia Cepacia ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Klebsiella Oxytoca ◽  
Antibiotic Sensitivity ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
Bank Note

Aims: The aim of this study was to characterize bacteria isolated from circulating Kenyan banknotes and also antibiotic susceptibility profiles within Nyeri County. Study Design:  This was a cross-sectional study and simple random sampling was used to collect 25 of each paper currency denomination. Place and Duration of Study: Samples analyses were done at Outspan Teaching and Referral Hospital (OTRH) laboratory, between March, 2019 and April, 2019. Methodology: Total of 125 currencies of five different denominations were collected from different marketing sources such as Butcheries, Restaurants, Health facilities, Mpesa outlets and Transport Saccos and dropped in sterile bags. The bacterial isolates were characterized on the basis of their morphology, staining and biochemical tests. Antibiotic sensitivity tests were done by Kirby Bauer disc diffusion technique. Results: Total of 19 different bacterial species were isolated from five Kenyan Bank note currencies. Of these, 37 (52.2%) was Staphylococcus aureus followed by Staphylococcus sciuri ssp.lentus 7 (9.9%), Staphylococcus gallinarum 2 (2.8%), Staphylococcus intermedius 6 (8.5%), Micrococcus sp. 1 (1.4%), Staphylococcus schleiferi ssp.coagulans 2 (2.8%), Staphylococcus sciuri ssp.rodentium 1 (1.4%), Kluyvera ascorbata 1 (1.4%), Proteus penneri 1 (1.4%), Aeromonas media 3 (4.2%), Burkholderia cepacia ssp.komplex (1.4%), Aeromonas enteropelogenes 1 (1.4%), Enterobacter cloacae 1 (1.4%), Klebsiella oxytoca 2 (2.8%), Leclercia adecarboxylata 1 (1.4%), Raoultella ornithinolytica 1 (1.4%), Vibrio metschnikovii  1 (1.4%), Myroides odoratus 1 (1.4%) and Yersinia pestis 1 (1.4%). Overall gram positive and gram negative bacterial isolates exhibited resistance to vancomycin, clindamycin and amoxycilin with percentages 40 (71%), 28 (50%), and 37 (66%) and 9 (64%), 8 (57%) and 6 (43%)  respectively. Conclusion: This study revealed that Kenyan banknote currencies circulating in Nyeri County were contaminated with different pathogenic and potential pathogenic bacteria including multi drug resistant strains. Hence, great care must be taken while handling money during the preparation and handling of food to avoid cross contamination.

scholarly journals Identification and antibiogram study of bacterial species isolated from milk samples of different locations in Bangladesh

2016 ◽  
Vol 1 (3) ◽  
pp. 457-462 ◽  
Author(s):  
Md Nuruzzaman Munsi ◽  
Nathu Ram Sarker ◽  
Razia Khatun ◽  
Mohammed Khorshed Alam
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Public Health Importance ◽  
Milk Samples ◽  
First Choice

Cow’s milk containing pathogenic bacteria is an important threat to the consumers. The objectives of the present study were to identify the bacterial agents of public health importance in milk samples (n=35) of different locations and to determine their sensitivity to different antibiotics. The milk samples were collected and transported aseptically and subsequently allowed for culture in bacteriological media, Gram’s staining and biochemical tests for the identification of bacterial species. The bacteria identified were Staphylococcus aureus, Escherichia coli and Salmonella typhi, and their prevalence, in case of vendor milk specimens (n=28), were 96.43%, 53.57% and 35.71% respectively, and of brand milk specimens (n=7), were 42.86 %, 28.57% and 0%, respectively. This suggests that cautionary measures should be taken for quality milk production and consumption. The antibiotic sensitivity test was done by disc diffusion method and the average inhibition zones, in case of Staphylococcus aureus, were 32 mm for oxytetracycline, 26 mm for amoxicillin, 35 mm for ciprofloxacin, 27 mm for cefotaxime, 30 mm for ceftriaxone, 30 mm for azithromycin, and 26 mm for erythromycin; in case of Escherichia coli, were 5 mm for oxytetracycline, 9 mm for amoxicillin, 22 mm for ciprofloxacin, 30 mm for cefotaxime, 31 mm for ceftriaxone, 15 mm for azithromycin, and 0 mm for erythromycin; in case of Salmonella typhi., were 25 mm for oxytetracycline, 24 mm for amoxicillin, 38 mm for ciprofloxacin, 31 mm for cefotaxime, 34 mm for ceftriaxone, 24 mm for azithromycin, and 0 mm for erythromycin. Therefore, ciprofloxacin and ceftriaxone may be the antibiotics of first choice, and cefotaxime and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.Asian J. Med. Biol. Res. December 2015, 1(3): 457-462

scholarly journals Bacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculture

2020 ◽  
Vol 8 (9) ◽  
pp. 1343
Author(s):  
Vanessa Salgueiro ◽  
Vera Manageiro ◽  
Narcisa M. Bandarra ◽  
Lígia Reis ◽  
Eugénia Ferreira ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Sparus Aurata ◽  
Bacterial Species ◽  
Growth Potential ◽  
Quinolone Resistance ◽  
World Health ◽  
Bacterial Strains ◽  
Health Organization ◽  
Skin Samples

In a world where the population continues to increase and the volume of fishing catches stagnates or even falls, the aquaculture sector has great growth potential. This study aimed to contribute to the depth of knowledge of the diversity of bacterial species found in Sparus aurata collected from a fish farm and to understand which profiles of diminished susceptibility to antibiotics would be found in these bacteria that might be disseminated in the environment. One hundred thirty-six bacterial strains were recovered from the S. aurata samples. These strains belonged to Bacillaceae, Bacillales Family XII. Incertae Sedis, Comamonadaceae, Enterobacteriaceae, Enterococcaceae, Erwiniaceae, Micrococcaceae, Pseudomonadaceae and Staphylococcaceae families. Enterobacter sp. was more frequently found in gills, intestine and skin groups than in muscle groups (p ≤ 0.01). Antibiotic susceptibility tests found that non-susceptibility to phenicols was significantly higher in gills, intestine and skin samples (45%) than in muscle samples (24%) (p ≤ 0.01) and was the most frequently found non-susceptibility in both groups of samples. The group of Enterobacteriaceae from muscles presented less decreased susceptibility to florfenicol (44%) than in the group of gills, intestine and skin samples (76%). We found decreased susceptibilities to β-lactams and glycopeptides in the Bacillaceae family, to quinolones and mupirocin in the Staphylococcaceae family, and mostly to β-lactams, phenicols and quinolones in the Enterobacteriaceae and Pseudomonadaceae families. Seven Enterobacter spp. and five Pseudomonas spp. strains showed non-susceptibility to ertapenem and meropenem, respectively, which is of concern because they are antibiotics used as a last resort in serious clinical infections. To our knowledge, this is the first description of species Exiguobacterium acetylicum, Klebsiella michiganensis, Lelliottia sp. and Pantoea vagans associated with S. aurata (excluding cases where these bacteria are used as probiotics) and of plasmid-mediated quinolone resistance qnrB19-producing Leclercia adecarboxylata strain. The non-synonymous G385T and C402A mutations at parC gene (within quinolone resistance-determining regions) were also identified in a Klebsiella pneumoniae, revealing decreased susceptibility to ciprofloxacin. In this study, we found not only bacteria from the natural microbiota of fish but also pathogenic bacteria associated with fish and humans. Several antibiotics for which decreased susceptibility was found here are integrated into the World Health Organization list of “critically important antimicrobials” and “highly important antimicrobials” for human medicine.

scholarly journals Bacteriological Etiology of Flood Affected Diarrhoeal Patients Admitted in Dhaka Medical College Hospital and Antibiogram of the isolates

2016 ◽  
Vol 2 (1) ◽  
pp. 13-17
Author(s):  
Abdur Rahman ◽  
Aliya Rashid ◽  
Shaheen Ara Begum ◽  
Aktheruzzaman Chowdhury ◽  
Shahin Ara Begum ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Age Groups ◽  
Medical College Hospital ◽  
Diffusion Method ◽  
Aeromonas Species ◽  
Biochemical Tests ◽  
College Hospital ◽  
E Coli ◽  
Medical College ◽  
Almost All

Diarrhoea is a self-limiting  disease  caused by various  enteropathogens and antimicrobial  therapy  is  an  important adjunct  to  fluid  therapy  in  the  management of the  cases.  We have studied  the  flood-affected  diarrhoeal  (post-flood diarrhoea) patients  admitted in  Dhaka Medical College Hospital  during  August to September,  2007.  A total  of ll4 faecal  samples  were  collected  from  the  patients of all age  groups,  and  investigated  for  bacterial  enteropathogens. Specimens  were cultured  in  appropriate  media and  identification  of the  organisms  showing  growth  was done  by relevant  biochemical  tests.  In addition, the isolates of Vbrio cholera wer confirmed  using  pollvalent  antisera. Sensitivity of the isolated organisms was done by disc diffusion method.  Among the isolated bacterial enteropathogens, majority was V. cholera (46, 40.3%). Other  isolates  included Escherichia coli (18,  23.0%),  Aeromonas species  (8,10.O%)  and V parahaemolyticus  (5,  7.0%). No pathogenic bacteria were isolated from 27 of the cases.  No Salmonella or Shigella was detected in any case. Majority of the cases  (24, 52.0%)  infected  with V cholerae  were  children  having  less than  L0 years of  age. Most of the isolates of V cholerae (77.7%) were sensitive to Ciprofloxacin.  Almost all of the V cholerae isolates (97.90 ) were resistant to Tetracycline, followed by Co-trimoxazloe (93,5%)  and  Nalidixic  acid (59.2%).  All of the E. coli isolates (100.0%) were sensitive to Ceftriaxone, followed by Ceftazidime (88.8%) and Ciprofloxacin (77.7oh).  Thus it  can  be concluded  that  Ciprofloxacin and Ceftriaxone  can  be considered  as the drugs  ofchoice  for  the  treatment  of  post-flood  diarrhoea  along  with proper  rehydration  therapy.Bangladesh  J Med Microbiol2008;  02 (01):13-17DOI: http://dx.doi.org/10.3329/bjmm.v8i1.21929

Antimicrobial susceptibility of clinical Staphylococcus aureus isolated from patients with an ear infection in Misan

2018 ◽  
Vol 9 (3) ◽  
pp. 1016
Author(s):  
Munaf Aal-Aaboda ◽  
Mohammed R. Al-Notazy
Keyword(s):  
Antibiotic Susceptibility ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Antibiotics Resistance ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Ear Infection ◽  
Mannitol Salt Agar ◽  
Causative Agents ◽  
High Level

The present study was targeted to examine the prevalence of multi-drug resistant Staphylococcusaureus,which has been carried out in Misan, Iraq at a local hospital from February 2016 to January 2017.A hundred and eighty ear swabs have been obtained from patients with ear infections with or without discharges. Culturing and identifying the causative agents, as well as the antibiotic sensitivity profile, have been done on the specimens. Swabs were collected under sterile conditions and instantly transferred to the laboratory sealed in brain heart broth tubes. The initial isolation was done on selective media to S. aureus(mannitol salt agar) at a temperature of 37°C for 24 - 48 hours and then the biochemical tests and identification were done in accordance with the standard monotonous techniques. Antibiotic susceptibility tests were done by the disk diffusion method. A hundred and forty-four isolates diagnosed with Staphylococcus aureusand eighteen isolates as other bacteria. S.aureusisolates tested for antibiotic susceptibility showed high resistance to ampicillin, carbenicillin and amoxicillin, mild resistance to co-trimoxazole and were susceptible to norfloxacin, rifampicin, and ciprofloxacin.Additionally, S.aureusisolates showed multiple antibiotic resistance (MAR). The MAR index of the isolates found to range between 0.35 and 0.7. In conclusion, an ear infection is mostly caused by Staphylococcus aureusand most of these isolates showed a high level of antibiotics resistance, which eventually may lead to too many health-related consequences in Misan, Southern Iraq and expose the needs for further studies to lessen the resistance to antibiotics.

scholarly journals Study of Bacterial pathogens in Urinary Tract Infection and their antibiotic resistance profile in a tertiary care hospital of Bangladesh

2017 ◽  
Vol 10 (1) ◽  
pp. 22-26
Author(s):  
Sanjida Khondakar Setu ◽  
Abu Naser Ibne Sattar ◽  
Ahmed Abu Saleh ◽  
Chandan Kumar Roy ◽  
Mushtaque Ahmed ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Antibiotic Susceptibility ◽  
Bacterial Species ◽  
Tertiary Care ◽  
Resistant Bacteria ◽  
Antibiotics Resistance ◽  
Care Hospital ◽  
Biochemical Tests ◽  
Antibiotic Resistance Profile ◽  
Tract Infections

Analyzing antibiotic susceptibility pattern of uropathogens help to overcome the therapeutic difficulties created by the rising antimicrobial resistant bacteria and guides in choosing appropriate antibiotics. Hence, we aimed at evaluating the pathogens causing UTI and study their antibiogram. Midstream urine samples were collected, cultured and appropriate biochemical tests were performed for proper identification of urinary pathogens in BSMMU from January 2013 to December 2013. The most common isolated Gram negative uropathogens were Escherichia coli (63.93%) followed by Klebsiella pneumoniae (17.09%), other bacterial species, named Pseudomonas spp. Enterobacter Acinetobacter spp. Citrobacter spp Proteus spp. Morganella. Among Gram positive organism S. aureus S. saprophyticus S. agalectiae and Enterococci were found. Urinary tract infections were more prevalent in women than men (61.68% vs. 38.32%). High level of sensitivity was found to imipenem, amikacin, nitrofurantoin, ceftriaxone, gentamicin, cefuroxime in most of the isolates. Almost all the test organisms exhibited multiple antibiotic resistances. The high multiple antibiotics resistance identified makes it necessary for antibiotic susceptibility testing to be conducted prior to antibiotic(s) prescription.Bangladesh J Med Microbiol 2016; 10 (01): 22-26

scholarly journals Identificación bacteriana en teléfonos celulares de estudiantes de medicina que acuden o no, a un Hospital General en Mérida, Yucatán, México

2019 ◽  
pp. 21-25
Author(s):  
Angel D. CAAMAL-LEY ◽  
Miguel A. PUC-FRANCO ◽  
Mario R HEREDIA-NAVARRETE ◽  
David LINDO-PEREZ ◽  
Alberto VARGAS-GONZALEZ
Keyword(s):  
Information Exchange ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Cell Phones ◽  
Bacterial Species ◽  
Isotonic Saline ◽  
Medical Personnel ◽  
Biochemical Tests ◽  
Gram Staining ◽  
First Year Medical Students

The cell phone is an important tool for communication, consultation and information exchange; Studies carried out in hospitals have shown that cell phones of medical personnel are contaminated by nosocomial pathogens. However, the bacterial species that the cell phones house and their potential risk have not been determined. We analized the cell phones of 30 first-year medical students who do not visit the hospital in their studies vs. 30 students who perform their clerkship practices in hospitals, to determine the differences in bacterial loads. Samples were taken by sterile swabs moistened with isotonic saline. McConkey and salt-mannitol agar were used as culture media. The biochemical tests used for identification of enterobacteria were citrate, MIO, LIA, urea and TSI. Degradation tests of mannitol, coagulase, catalase and oxidase were used for the identification of staphylococci. Likewise, smears and Gram staining of the isolated colonies were performed. Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli and Pseudomona sp. were mainly found. Differences in number and bacterial species were found in the cell phones of each study group, representing a reservoir of potentially pathogenic bacteria for humans.

scholarly journals Identification and antibiotic susceptibility of bacterial microbiota of freshwater fish

10.5219/1063 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 408-414
Author(s):  
Alina Klūga ◽  
Miroslava Kačániová ◽  
Margarita Terentjeva
Keyword(s):  
Antibiotic Susceptibility ◽  
Negative Impact ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Total Bacterial Count ◽  
Pseudomonas Spp ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Fish Samples

The fish meat is an essential part of human diet. However, fish may be contaminated with different microorganisms, including pathogens. Antimicrobial resistance of fish microbiota may facilitate the spread of resistant microorganisms causing serious consequences for human health. The aim of the present study was to detect bacterial contamination in fish gill, gut and skin and to determine antimicrobial susceptibility of the bacterial isolates. Rainbow trout (Oncorhynchus mykiss) and bream (Abramis bram) were obtained from the market in Jelgava city. Chub (Leuciscus cephalus), crucian carp (Carassius carassius) and tench (Tinca tinca) were collected from fishermen. Fish samples were examined for the total bacterial count (TBC), coliforms, Enterobacteriaceae, Pseudomonas spp. and Aeromonas spp. Testing was done in accordance with International Organization for Standardization (ISO) standards. Identification of all bacteria was accomplished with the Matrix Assisted Laser Desorption Ionization – Time of Flight Mass Spectrometry (MALDI-TOF MS) method. The disc diffusion method was used for the detection of antibiotic susceptibility of isolated bacterial species. TBC ranged from 2.70 to 7.00 log CFU.g-1, coliforms from 0 to 2.67 log CFU.g-1, Enterobacteriaceae from 0 to 2.85 log CFU.g-1. The highest contamination with Pseudomonas spp. and Aeromonas spp. was observed in chub gut samples with 1.60 log CFU.g-1 and 2.23 log CFU.g-1, respectively. Altogether, 16 microbial genera and 31 bacterial species were identified. The dominant bacterial species belonged to Pseudomonas spp. (54%) and Enterobacteriaceae. Pseudomonas spp. were resistant to ticarcillin, susceptibility to ciprofloxacin showed 88% of isolates. All Enterobacteriaceae isolates were susceptible to imipenem. The microbial quality of the fish was acceptable, but the presence of antibiotic resistant bacteria may further cause a negative impact on public health.

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