scholarly journals Evaluating the Resistance to the Plum Pox Virus of Some Apricot Tree Cultivars and Hybrids in South-Eastern Romania

2013 ◽  
Vol 67 (2) ◽  
pp. 203-206
Author(s):  
Marioara Trandafirescu ◽  
Liana Melania Dumitru ◽  
Iulian Trandafirescu
Keyword(s):  
Elisa Test ◽  
Contamination Level ◽  
Plum Pox Virus ◽  
Research Station ◽  
Serological Method ◽  
Fruit Growing ◽  
Apricot Tree ◽  
Very High ◽  
Selection Of ◽  
Considerable Damage

In Europe, the Plum pox virus causes one of the most serious diseases of stone fruit-growing species (plum, apricot, peach, nectarine, almond and cherry) in all countries where cultivated. Romania is one of the countries in which this virus has been present for several years and where the contamination level is very high. The disease causes considerable damage, as the infected trees have few fruit, which are small, deformed, lacking in flavour, with a low content of sugar and which can neither be consumed nor used in production. In areas where the Plum pox virus is present, the cultivation of highly resistant (tolerant) genotypes is the only possibility to alleviate this problem. The purpose of the research performed in this paper was to identify varieties that are tolerant to the virus and to monitor the manner in which the resistance is transmitted to hybrid descendants. The material employed consisted of apricot tree varieties from the national collection of the Research Station for Fruit-Growing Constanþa. The presence of the Plum pox virus was determined by biological method using a GF305 wooden indicator and the serological method through the ELISA test. The varieties identified as having higher resistance to the attack of the Plum pox virus (‘Stark Early Orange’, ‘NJA 17’, and ‘Sulmona’) were used in intraspecific hybridisations for the selection of hybrids to which this characteristic was transmitted. These included: the selections ‘VT 48/45’, ‘VT 51/45’, ‘VT 47/112’ and ‘H 9/5’.

scholarly journals Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch

2019 ◽  
Vol 67 (1) ◽  
pp. 121-131
Author(s):  
Petra Pavelková ◽  
Tomáš Kiss ◽  
Tomáš Nečas
Keyword(s):  
Real Time ◽  
Growing Season ◽  
Elisa Test ◽  
Plum Pox Virus ◽  
Rt Pcr ◽  
Serological Method ◽  
Low Concentrations ◽  
Symptom Intensity ◽  
Das Elisa ◽  
Prunus Davidiana

Evaluation of the presence and concentration of PPV (Plum pox virus) in selected rootstocks was carried out in 2016–2017. For the purpose of the experiment we used rootstocks derived from crossbreeding of Prunus davidiana (Carr.) Franch, such as Cadaman and Barrier, and also a P. davidiana seedling. Peach seedling rootstock GF‑305 was used as a control. The rootstocks were inoculated artificially with PPV strain M (Marcus). Both the rootstock and the inoculum were tested for presence of the virus by a serological method – semiquantitative DAS‑ELISA test and molecular methods – RT‑PCR, real‑time RT‑PCR and RT‑LAMP. During the growing season the plants were evaluated for symptom intensity by using a scoring scale. The results show interdependency between symptom intensity and the amount of PPV in leaves, with DAS‑ELISA test giving less positive samples than RT‑PCR. The RT‑LAMP and real‑time RT‑PCR methods were capable of revealing low concentrations of the virus even in symptom‑free plants. The lowest PPV concentrations of all the four rootstocks were detected by real‑time RT‑PCR in P. davidiana. The highest PPV concentrations were detected in Barrier rootstock. In inocula, the lowest concentration was found in the inocula on Cadaman rootstock, whereas the highest PPV concentration was detected in the inocula inoculated on Barrier rootstock.

Der Sommer und Herbst 2003 aus phänologischer Sicht | Summer and autumn 2003 from a phenological point of view

2004 ◽  
Vol 155 (5) ◽  
pp. 142-145 ◽  
Author(s):  
Claudio Defila
Keyword(s):  
Time Series ◽  
New Record ◽  
Point Of View ◽  
Late Spring ◽  
Mean Deviation ◽  
Leaf Shedding ◽  
The Mean ◽  
Very High ◽  
Selection Of

The record-breaking heatwave of 2003 also had an impact on the vegetation in Switzerland. To examine its influences seven phenological late spring and summer phases were evaluated together with six phases in the autumn from a selection of stations. 30% of the 122 chosen phenological time series in late spring and summer phases set a new record (earliest arrival). The proportion of very early arrivals is very high and the mean deviation from the norm is between 10 and 20 days. The situation was less extreme in autumn, where 20% of the 103 time series chosen set a new record. The majority of the phenological arrivals were found in the class «normal» but the class«very early» is still well represented. The mean precocity lies between five and twenty days. As far as the leaf shedding of the beech is concerned, there was even a slight delay of around six days. The evaluation serves to show that the heatwave of 2003 strongly influenced the phenological events of summer and spring.

scholarly journals Essential Oils of Four Virginia Mountain Mint (Pycnanthemum virginianum) Varieties Grown in North Alabama

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1397
Author(s):  
William N. Setzer ◽  
Lam Duong ◽  
Trang Pham ◽  
Ambika Poudel ◽  
Cuong Nguyen ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
University Research ◽  
Oil Yield ◽  
Research Station ◽  
Continuous Extraction ◽  
Chromatographic Techniques ◽  
Relative Differences ◽  
Essential Oil Yield ◽  
Very High

Virginia mountain mint (Pycnanthemum virginianum) is a peppermint-flavored aromatic herb of the Lamiaceae and is mainly used for culinary, medicinal, aromatic, and ornamental purposes. North Alabama’s climate is conducive to growing mint for essential oils used in culinary, confectionery, and medicinal purposes. There is, however, a need for varieties of P. virginianum that can be adapted and easily grown for production in North Alabama. Towards this end, four field-grown varieties with three harvesting times (M1H1, M1H2, M1H3; M2H1, M2H2, M2H3; M3H1, M3H2, M3H3, M4H1, M4H2, M4H3) were evaluated for relative differences in essential oil yield and composition. Thirty-day-old greenhouse-grown plants of the four varieties were transplanted on raised beds in the field at the Alabama A & M University Research Station in North Alabama. The plots were arranged in a randomized complete block with three replications. The study’s objective was to compare the four varieties for essential oil yield and their composition at three harvest times, 135, 155, and 170 days after planting (DAP). Essential oils were obtained by hydrodistillation with continuous extraction with dichloromethane using a Likens–Nickerson apparatus and analyzed by gas chromatographic techniques. At the first harvest, the essential oil yield of the four varieties showed that M1H1 had a yield of 1.15%, higher than M2H1, M3H1, and M4H1 with 0.91, 0.76, and 1.03%, respectively. The isomenthone concentrations increased dramatically through the season in M1 (M1H1, M1H2, M1H3) by 19.93, 54.7, and 69.31%, and M3 (M3H1, M3H2, M3H3) by 1.81, 48.02, and 65.83%, respectively. However, it increased only slightly in M2 and M4. The thymol concentration decreased slightly but not significantly in all four varieties; the thymol in M2 and M4 was very high compared with M1 and M3. The study showed that mountain mint offers potential for production in North Alabama. Two varieties, M1 and M3, merit further studies to determine yield stability, essential oil yield, composition, and cultivation development practices.

PSXII-2 Identification of candidate SNPs associated with high prolificacy in Romanov sheep

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 254-255
Author(s):  
Alexey V Shakhin ◽  
Arsen V Dotsev ◽  
Tatiana E Deniskova ◽  
Gottfried Brem ◽  
Natalia A Zinovieva
Keyword(s):  
Higher Education ◽  
Genetic Basis ◽  
Reference Genome ◽  
Unique Property ◽  
Candidate Snps ◽  
Merino Sheep ◽  
Sheep Breeds ◽  
Important Trait ◽  
Very High ◽  
Selection Of

Abstract Prolificacy is a very important trait in sheep. Romanov sheep, the well-known Russian sheep breed, are characterized by very high prolificacy; however, the genetic basis of this unique property of Romanov sheep is still unknown. It was reported that Ovine BMPR1B gene, located on the OAR6, is associated with prolificacy in several sheep breeds. The aim of our study was to identify candidate SNPs within BMPR1B gene, related to prolificacy. To achieve this goal, using NGS technology, we sequenced ovine BMPR1B gene in Romanov sheep (n = 6), which are characterized by high prolificacy (about 270 lambs per 100 ewes). The sequences of BMPR1B gene of Noire du Velay, Tan, Southdown and Australian Horned Merino sheep breeds as well as Asiatic mouflon (n = 1), which are characterized by significantly lower prolificacy (from 110 to 180 lambs per 100 ewes) were derived from publicly available sources and used for comparison. FST analysis performed in PLINK 1.9 program revealed 10 SNPs with values higher than 0.8. The majority of candidate SNPs under putative selection were localized in the region from 29,382,098 to 29,430,387 on OAR6 of Ovine reference genome (Oar_v3.1 (Ensembl release 98). Thus, we can suggest, that this region of the BMPR1B gene can be considered as the putative region, associated with high prolificacy of Romanov sheep. Additional studies will be needed to confirm the effect of identified candidate SNPs on prolificacy traits. The research results will be useful for artificial selection of sheep with higher prolific capacity, including the introduction of desired alleles in sheep populations using genome editing technologies. This work was supported by the Russian Ministry of Science and Higher Education No. 0445-2019-0024 and RFBR No. 20-516-56002.

scholarly journals Selection of Lentil (Lens culunaris L.) Lines Grown at Different Sowing Dates for Their Precocity and Their Agronomic Performance under Semi-arid Climate of Tunisia

2021 ◽  
Author(s):  
A. Ouji ◽  
M. Mechri ◽  
S. Wassli ◽  
K. Shiv ◽  
M. Kharrat
Keyword(s):  
Arid Zone ◽  
Sowing Date ◽  
High Yield ◽  
Research Station ◽  
Short Cycle ◽  
Cropping Season ◽  
Sowing Dates ◽  
Significant Yield ◽  
Semi Arid ◽  
Selection Of

Background: In Tunisia, water deficit and heat stress during the end of cycle are more frequent and causes significant yield losses. Selection of short cycle lines could be a good solution to escape the effect of heat and drought during the end of cycle. Furthermore, there is little published information on the effect of the sowing date on yield and its components in lentils. The aim of this study was to investigate the effect of the sowing date on yield and its components of some lentil lines. The best productive and early lines will be selected and therefore proposed for registration in the official catalog of plant varieties. The availability of these varieties to farmers could increase lentil production. Methods: Sixteen genotypes of lentil out of which 14 were advanced lines and 2 were checks varieties were used in this study. The field experiment was conducted during the 2017-2018 cropping season at Kef research station located in a semi-arid zone in north western Tunisia. Genotypes were sown on December 15th, 2017 and February, 7th, 2018. Seventeen agro-morphological parameters were recorded. Result: Based on agro-morphological analysis, lentil lines exhibited considerable genetic variability. Among the tested lines, L3 line seem to be the earliest lines. It showed high yield as well in timely and late sown dates. This line deserves more attention to develop short-cycle and high yielding variety.

scholarly journals Selection of non-invasive load procedure in diagnosis of ischemic heart disease (review)

2018 ◽  
pp. 62-70 ◽  
Author(s):  
V. P. Lupanov
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Clinical Examination ◽  
Ischemic Heart ◽  
Non Invasive ◽  
Careful Clinical Examination ◽  
Very High ◽  
Selection Of ◽  
High Pretest Probability

The diagnosis of stable ischemic heart disease begins with a careful clinical examination of the patient and non-invasive testing to identify the disease. Patients with very low and very high pretest probability should not undergo various non-invasive tests. Various non-invasive tests are available to assess the presence of coronary heart disease in patients with an intermediate probability of ischemic heart disease (15–65%). The combination of anatomical with functional non-invasive tests helps improve diagnostic capabili of the disease.

scholarly journals Изучение увологических и агробиологических показателей сорта винограда Кокур белый на различных подвоях для проведения клоновой селекции

2019 ◽  
pp. 105-108
Author(s):  
Nataliya Studennikova ◽  
Zinaida Kotolovets ◽  
Nadezhda Tikhomirova ◽  
Natalia Urdenko
Keyword(s):  
Training System ◽  
Large Percentage ◽  
Individual Selection ◽  
Grapevine Variety ◽  
Points Of View ◽  
Relative Productivity ◽  
Very High ◽  
Production Conditions ◽  
Selection Of

Представлены результаты работы по изучению влияния филлоксероустойчивых подвоев Берландиери × Рипариа Кобер 5ББ и Берландиери × Рипариа Телеки 4Б (СО4) на агробиологические и увологические показатели крымского технического белоягодного аборигенного сорта винограда Кокур белый в условиях Алуштинской долины (филиал «Алушта» ФГУП «ПАО «Массандра»). Для проведения исследований были выбраны: участок № 361, площадью 4,49 га, на котором произрастают кусты винограда Кокур белый, привитые на подвое Берландиери × Рипариа Кобер 5 ББ (2010 год посадки, схема 3 м ×1,25 м) и участок № 391, площадью 3,06 га, на котором возделываются растения сорта Кокур белый, привитые на подвое Берландиери × Рипариа Телеки 4Б (СО4) - 2011 год посадки, схема 3 м × 1 м). На обоих участках система ведения кустов - вертикальная трехпроволочная шпалера, формировка АЗОС-1, высота штамба - 1,3 м. Показана целесообразность проведения работ по улучшению сорта Кокур белый методом клоновой селекции на сортоподвойной комбинации Кокур белый подвой Кобер 5 ББ: у растений отмечен высокий уровень коэффициента плодоношения (0,92), довольно крупные грозди (365 г), урожай с куста в среднем составил 4,74 кг/куст, очень высокая продуктивность побега по сырой массе грозди - 336,6 г/побег, большой процент выхода мякоти и сока - 91,3%. Оценка имеющихся сортоподвойных комбинаций в производственных условиях позволила провести индивидуальный отбор генотипически ценных в биолого-хозяйственном отношении форм растений.The paper reports on the findings of a study on the influence of phylloxera-resistant rootstocks ‘Berlandieri’בRiparia Kober 5BB’ and ‘Berlandieri’בRiparia Teleki 4B (СО4)’ on the agro-biological and uvological indicators of Crimean aboriginal grapevine variety used in winemaking ‘Kokur Beliy in the conditions of Alushta valley (branch of Alushta FGUP PAO Massandra). The following were selected for research: plot № 361 with an area of 4.49 hectares with bushes of ‘Kokur Beliy’ grafted on rootstock of ‘Berlandieri’ × Riparia Kobera 5 BB’ (planting year - 2010, planting scheme 3 m ×1.25 m) and plot № 391 covering 3.06 hectares with ‘Kokur Beliy’ vines grafted on ‘Berlandieri’ × ‘Riparia Teleki 4B (СО4)’ rootstock - planting year - 2011, planting scheme 3 m×1 m). On both plots, the training system used was vertical three-wire trellis, vines shaped as AZOS-1, the trunk height 1.3 m. The paper demonstrates the relevance of the work on improvement of ‘Kokur Beliy’ cultivar with the help of clonal breeding on rootstock cultivar combination ‘Kokur Beliy’ rootstock ‘Kober 5 BB’: the plants demonstrated high relative productivity (0.92), quite large bunches (365 g), yield per bush made on average 4.74 kg/bush, very high shoot productivity as per raw bunch weight 336.6 g/shoot, a large percentage of pulp and juice output - 91.3 %. Assessment of the cultivar-rootstock combinations under production conditions allowed making individual selection of genotypically valuable, from the biological and economic points of view, plants.

scholarly journals The Occurrence of Aflatoxin M1 in Milk, soft cheese and yoghurt in Baghdad Province by Using ELISA Test

2014 ◽  
Vol 38 (2) ◽  
pp. 9-16
Author(s):  
Najim Hadi Najim
Keyword(s):  
Dairy Products ◽  
Raw Milk ◽  
Elisa Test ◽  
Mean Value ◽  
Aflatoxin M1 ◽  
Contamination Level ◽  
Milk Samples ◽  
White Cheese ◽  
The Mean ◽  
Milk And Dairy Products

     Milk and dairy products are fundamental components in the human diet and may be the principle way for entrance of Aflatoxin M1 (AFM1) in to the human body. All milk and dairy products samples were tested for the occurrence of AFM1 by the competitive ELISA technique. Out of 32 bovine raw milk samples that were collected from eight villages around Baghdad province, 32 samples (100 %) were contaminated with AFM1 ranging from 0.15 to 86.96ng/kg with mean value of 42.37±26.07 ng/kg, of which 17 samples were contaminated with concentrations < 50 ng/kg and 15 samples exceeded the maximum acceptable level of AFM1 in milk (50 ng/kg) imposed by the European legislation. The raw milk samples belonged to animals fed with composite and stored fodder as in Althahab Alabiadh, Radhwaniya and Fadhaliya villages had higher significantly AFM1 concentrations over all the other five villages (Grazing feed). All 32 (100%) locally produced soft white cheese samples analyzed were contaminated with AFM1 ranging from 31.84 to 89.44 ng/kg with the mean value of 59.92±17.03 ng/kg. Out of 32 locally produced yoghurt samples analyzed, 32 samples (100%) were contaminated with AFM1 ranging from 0.16 to 42.74 ng/kg with the mean value of 16.92±11.55 ng/kg. Thirty samples (100%) of the examined 30 imported UHT milk samples that were collected from different commercial companies in the province of Baghdad presented significantly  high contamination level with AFM1 that were found to range from 0.18 to 85.66 ng/kg.

Genetic variation of rectal temperature and its relationship to milk production in Holstein dairy cows

2009 ◽  
Vol 2009 ◽  
pp. 63-63
Author(s):  
S Khalajzade ◽  
N Emam Jomeh ◽  
A Salehi ◽  
A Moghimi Esfandabadi
Keyword(s):  
Heat Stress ◽  
Dairy Cows ◽  
Milk Production ◽  
Heat Tolerance ◽  
Rectal Temperature ◽  
Weather Factors ◽  
And Performance ◽  
Very High ◽  
Selection Of ◽  
Holstein Dairy Cows

Milk production is significantly decreased by thermal stress. The survival and performance of an animal during heat stress periods depend on several weather factors, especially temperature and humidity. Researchers reported dramatic decreases in milk production as temperature rose above 30 degree of centigrade. Very high environmental temperature is common during the summer months in Iran. Rectal temperature is as indicator of heat tolerance and has been the most frequently used physiological variable for estimating heat tolerance in cattle. Some dairy cows are more heat tolerant and productive when subjected to heat stress. Identification and selection of heat stress resistant cattle offers the potential to increase milk yield in tropical environment. The aim of the present study was to estimate genetic parameters of heat tolerance and its relationship to milk production in Holstein Dairy Cows in Iran.

scholarly journals GIS technologies for selecting location of dams in the flood control systems

2019 ◽  
Vol 91 ◽  
pp. 07001 ◽  
Author(s):  
Mikhail Fedorov ◽  
Vladimir Badenko ◽  
Alexander Chusov ◽  
Vladimir Maslikov
Keyword(s):  
Control Systems ◽  
Flood Control ◽  
Control Volume ◽  
Flood Protection ◽  
Eastern Region ◽  
Engineering Structures ◽  
Gis Technologies ◽  
Far Eastern ◽  
Selection Of ◽  
Considerable Damage

Many floodplains are excluded from development because the floods cause considerable damage to people’s lives and properties. Systems of hydraulic engineering structures for flood protection working with hydropower facilities are analysed to solve the problem of reducing the flood control volume of hydropower station. Methods of reducing the risk of flooding in the river basin by means of a distributed system of detention self-regulated dams are discussed. A geoinformation method used to justify the selection of parameters of such dams, primarily location of dam that minimize impact on the environment (ecological factor), is presented. In the present study, a system of multipurpose flood protection self-regulated dams is analysed as the measures for mitigation of flash floods in the Far Eastern region of Russia.

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