scholarly journals Preliminary Study on the Safety of a New Vaccine Against Canine Babesiosis Containing Soluble Parasitic Antigen (SPA)

2012 ◽  
Vol 56 (2) ◽  
pp. 145-148 ◽  
Author(s):  
Łukasz Adaszek ◽  
Romana Wernicka-Furmaga ◽  
Stanisław Winiarczyk
Keyword(s):  
Clinical Observation ◽  
Control Group ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Biochemical Tests ◽  
Internal Organs ◽  
Appropriate Treatment ◽  
Preliminary Study ◽  
Local Reactions ◽  
Babesia Canis Canis

Abstract The aim of this study was to assess the safety of a new vaccine, containing soluble parasitic antigen (SPA), against canine babesiosis. Fifteen dogs were included in the experiment. Five controls received only the adjuvant and 10 dogs were vaccinated with Babesia canis canis SPA twice, at a two weeks interval. For the whole period of the study all animals were under constant clinical observation. Haematological and biochemical tests were performed. Flu-like symptoms and local reactions at the injection site were observed in three animals from the vaccinated group and in two dogs from the control group. These events were transient, receded spontaneously and did not require any appropriate treatment. In 50% of the vaccinated dogs, a slight and spontaneously receding thrombocytopenia developed. However, in none of the animals used in the experiment shock symptoms were observed. Administration of the SPA did not affect the functions of internal organs, which was confirmed by normal results of biochemical tests. The obtained Babesia canis SPA can be considered safe and well tolerated by dogs, and therefore it can be used in further studies on the immunisation of animals against babesiosis.

The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs

2013 ◽  
Vol 16 (1) ◽  
pp. 131-133 ◽  
Author(s):  
Ł. Adaszek ◽  
M. Jankowska ◽  
M. Kalinowski ◽  
T. Banach ◽  
D. Wułupek ◽  
...  
Keyword(s):  
High Specificity ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Loop Mediated Isothermal Amplification ◽  
Specificity And Sensitivity ◽  
Amplification Assay ◽  
Analytical Laboratories ◽  
Babesia Canis Canis

Abstract The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.

scholarly journals A field trial evaluation of the prophylactic efficacy of amitraz-impregnated collars against canine babesiosis (Babesia canis rossi) in South Africa

2007 ◽  
Vol 78 (2) ◽  
Author(s):  
R.D. Last ◽  
J.D. Hill ◽  
P.T. Matjila ◽  
C.A. Reme
Keyword(s):  
South Africa ◽  
Treatment Group ◽  
Geographical Area ◽  
Tick Control ◽  
Domestic Dog ◽  
Control Group ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Group 2 ◽  
Group 1

South African canine babesiosis caused by Babesia canis rossi is a common clinical disease in dogs in South Africa and remains a significant cause of domestic dog mortality. To determine whether tick-repellent, 9% amitraz-impregnated tick collars (Preventic-Virbac) could prevent tick-borne exposure to B. canis rossi, 50 dogs were assigned to two groups. Group 1 (20 dogs), polymerase chain reaction (PCR)- and reverse line blot (RLB)-negative for B. canis rossi, were fitted with amitraz collars and blood samples collected monthly , over a 6-month period, and analysed for B. canis rossi. Group 2 (30 dogs) included 5 dogs selected on a month-by-month basis from a population of dogs from the same geographical area as the group 1 dogs, but with no history of previous tick control, which were blood-sampled together with the treatment group and analysed for B. canis rossi by PCR and RLB, to serve as the control group. Eight of the 30 control dogs (26.6 %) were PCR / RLB positive for B. canis rossi, indicating high pathogen exposure during the trial period. All twenty of the treatment group dogs remained negative for B. canis rossi throughout the 6 months of the trial. These results suggest that the use of amitraz-impregnated collars had a significant effect on reducing infection with B. canis rossi.

In-clinic laboratory diagnosis of canine babesiosis (Babesia canis canis) for veterinary practitioners in Central Europe

2012 ◽  
Vol 40 (02) ◽  
pp. 87-94 ◽  
Author(s):  
M. Leschnik ◽  
E. Hooijberg ◽  
A. Tichy ◽  
E. Leidinger ◽  
G. Kirtz
Keyword(s):  
Reticulocyte Count ◽  
Climatic Factors ◽  
Laboratory Diagnosis ◽  
Climatic Conditions ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Vector Activity ◽  
Red Blood Cell Count ◽  
Haematological Changes ◽  
Babesia Canis Canis

Summary Objective: Haematological changes in dogs and climatic conditions favourable for the vector may assist in the quick in-house diagnosis of canine babesiosis. Material and methods: Blood samples from 358 dogs suspected to have canine babesiosis were evaluated. The diagnosis was confirmed in 113 dogs by detection of Babesia canis by microscopic examination of a stained blood smear using the concentration line technique. Results: Thrombocytopenia was present in all 113 dogs. Red blood cell count, packed cell volume and haemoglobin values were below the reference range in 62.8%, 61.1% and 46.0% of affected dogs, respectively. An increased reticulocyte count was apparent in five Babesia canis-positive dogs. Leukopenia, lymphopenia, neutropenia and monocytosis were present in 54.9%, 47.8%, 30.4% and 6.5% of the dogs, respectively. Evaluating haematological parameters by CARTanalysis revealed a predictive model (accuracy = 93.5%) for canine babesiosis, when using the leucocyte, thrombocyte, and reticulocyte count. Climatic conditions present at the most probable time of Babesia canis-infection accounted for biseasonal occurrence. Changes of climatic factors during the year influence the vector activity and in conclusion should highlight babesiosis in the ranking of differentials for veterinarians. Conclusion: The results demonstrate that a tentative diagnosis of canine babesiosis can be made based on typical haematological changes. The results recorded match well with the seasonality of the tick vector and were confirmed here by the month of sample submission.

scholarly journals Comparison of effects of uncomplicated canine babesiosis and canine normovolaemic anaemia on abdominal splanchnic Doppler characteristics - a preliminary investigation

2005 ◽  
Vol 76 (3) ◽  
Author(s):  
L.M. Koma ◽  
R.M. Kirberger ◽  
A.L. Leisewitz ◽  
L.S. Jacobson ◽  
P.J. Becker ◽  
...  
Keyword(s):  
Physiological State ◽  
Preliminary Investigation ◽  
Resistive Index ◽  
Control Group ◽  
Left Renal Artery ◽  
Canine Babesiosis ◽  
Mean Velocity ◽  
Diastolic Velocity ◽  
Haemodynamic Changes ◽  
Preliminary Study

A preliminary study was conducted to compare uncomplicated canine babesiosis (CB) and experimentally induced normovolaemic anaemia (EA) using Doppler ultrasonography of abdominal splanchnic vessels. Fourteen dogs with uncomplicated CB were investigated together with 11 healthy Beagles during severe EA, moderate EA and the physiological state as a control group. Canine babesiosis was compared with severe EA, moderate EA and the physiological state using Doppler variables of the abdominal aorta, cranial mesenteric artery (CMA), coeliac, left renal and interlobar, and hilar splenic arteries, and the main portal vein. Patterns of haemodynamic changes during CB and EA were broadly similar and were characterised by elevations in velocities and reductions in resistance indices in all vessels except the renal arteries when compared with the physiological state. Aortic and CMA peak systolic velocities and CMA end diastolic and time-averaged mean velocities in CB were significantly lower (P < 0.023) than those in severe EA. Patterns of renal haemodynamic changes during CB and EA were similar. However, the renal patterns differed from those of aortic and gastrointestinal arteries, having elevations in vascular resistance indices, a reduction in end diastolic velocity and unchanged time-averaged mean velocity. The left renal artery resistive index in CB was significantly higher (P < 0.025) than those in EA and the physiological state. Renal interlobar artery resistive and pulsatility indices in CB were significantly higher (P < 0.016) than those of moderate EA and the physiological state. The similar haemodynamic patterns in CB and EA are attributable to anaemia, while significant differencesmayadditionally be attributed to pathophysiological factors peculiar to CB.

Autochthonous canine babesiosis caused by Babesia canis canis in Latvia

2013 ◽  
Vol 196 (3-4) ◽  
pp. 515-518 ◽  
Author(s):  
Inese Berzina ◽  
Valentina Capligina ◽  
Viesturs Baumanis ◽  
Renate Ranka ◽  
Dina Cirule ◽  
...  
Keyword(s):  
Babesia Canis ◽  
Canine Babesiosis ◽  
Babesia Canis Canis

Molecular detection of Babesia canis in Dermacentor reticulatus ticks collected in Slovakia

Biologia ◽  
2006 ◽  
Vol 61 (2) ◽  
Author(s):  
Darja Duh ◽  
Mirko Slovák ◽  
Ana Saksida ◽  
Katja Strašek ◽  
Miroslav Petrovec ◽  
...  
Keyword(s):  
Francisella Tularensis ◽  
Coxiella Burnetii ◽  
Molecular Detection ◽  
Vector Competence ◽  
Dermacentor Reticulatus ◽  
Molecular Evidence ◽  
Babesia Canis ◽  
Aetiological Agent ◽  
Canine Babesiosis ◽  
Babesia Canis Canis

AbstractDermacentor reticulatus ticks are recognized as the most important vectors of Babesia canis, the aetiological agent of canine babesiosis occurring throughout Europe. Vector competence of D. reticulatus for B. canis is well described and experimentally determined; however, by using molecular analysis it was proven so by one recent study in Russia. Herein, the additional molecular evidence of B. canis infection in D. reticulatus ticks collected in Slovakia is provided. Using PCR followed by sequencing of distinctive amplicons we determined the presence of Babesia canis canis in one of 100 tested adult ticks. Two zoonotic pathogens, Francisella tularensis and Coxiella burnetii, were previously isolated from D. reticulatus ticks in Slovakia. In our samples, we detected only the presence of F. tularensis.

Alterations in some blood coagulation parameters in naturally occurring cases of canine babesiosis

2009 ◽  
Vol 57 (2) ◽  
pp. 295-304 ◽  
Author(s):  
Renata Rafaj ◽  
Vesna Matijatko ◽  
Ivana Kiš ◽  
Nada Kučer ◽  
Tatjana Živičnjak ◽  
...  
Keyword(s):  
Antithrombin Iii ◽  
Activated Partial Thromboplastin Time ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Coagulation Parameters ◽  
Naturally Occurring ◽  
At Iii ◽  
Healthy Dogs ◽  
Babesia Canis Canis

Changes in coagulation parameters were studied in dogs naturally infected with Babesia canis canis (n = 30), and haemostasis was evaluated and compared to values obtained from healthy dogs (n = 29). To date, there have not been any studies examining the dynamics of thrombin-antithrombin complex formation in cases of canine babesiosis. Coagulation parameters evaluated before (day 0) and on days 1, 2, and 3 after treatment with imidocarb (6 mg/kg inj. s.c.) included the determination of platelet counts, the formation of thrombin-antithrombin complexes (TAT), prothrombin time (PT), activated partial thromboplastin time (APTT) and antithrombin III (AT III) activity. TAT complexes were significantly elevated in animals with babesiosis on days 0 and 2 (mean 49.7 and 87.7 μg/L vs. control, 7.2 μg/L). AT III activity was significantly decreased at all time-points examined. There were no differences in PT. On days 2 and 3 the APTT was significantly shortened in the infected dogs when compared to control animals (means of 21.3 and 19.2 s vs. control, 30.0 s). Our analysis demonstrated that infected dogs had significant thrombocytopenia during the course of the study (mean day 0 − 29 × 10 9 /L, day 1 − 48 × 10 9 /L, day 2 − 47 × 10 9 /L and day 3 − 87 × 10 9 /L, vs. control −259 × 10 9 /L). These data suggest that babesiosis in dogs compromise primary and secondary haemostasis and that induction of disseminated intravascular coagulation (DIC) occurs in canine babesiosis.

The Toxicity of Castor Beans and its Treatment with Doxycycline in Local Rabbits

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Nael Mohammed Sarheed ◽  
Osamah Faisal Kokas ◽  
Doaa Abd Alabas Muhammed Ridh
Keyword(s):  
Castor Bean ◽  
The Body ◽  
Control Group ◽  
High Concentration ◽  
Biochemical Tests ◽  
Doxycycline Treatment ◽  
High Concentrations ◽  
Physiological Tests ◽  
Animal House ◽  
Physiological And Biochemical

The plant of castor is widely spread in the Iraqi land, and characterized with containing ricin toxin, which has a very serious effects, and because the seeds of this plant scattered in the agricultural soil and rivers water, which increases the exposure of humans and animals to these beans. Objective: This experiment was designed to study the effect of high concentration of castor bean powder in some physiological and biochemical parameters and changes in some tissues of the body, as well as trying to use doxycycline to reduce the effects of ingestion of these seeds. Materials and Methods: In the experiment, 24 local rabbits were raised and fed in the Animal House of the Faculty of Medicine / Al-Muthanna University, then divided into four groups and treated for three weeks (21 days), Control group: treated with normal saline solution (0.9) orally throughout the experiment, G1: was treated orally with a concentration of 25 mg / kg of castor bean powder daily during the experiment, G2 : orally treated 25 mg / kg of castor bean and 25 mg / kg of doxycycline, G3: orally treated 25 mg / kg of castor powder with 50 mg / kg of doxycycline daily throughout the trial period. Results: The results of the experiment showed significant changes (P less than 0.05) in all physiological and biochemical blood tests when compared with control group. There was a significant decrease in PCV, Hb, RBC, T.protein and body weights, while demonstrated a significant increase in WBC, Urea, Creatinine, ALT, AST and ALP, with distortions in liver and kidney of animals that treated with Castor beans. In contrast, the treatment with doxycycline and caster beans showed significant improvement reflected by a normal proportion in physiological tests and biochemical tests with improvement in the tissues when compared to control group. Conclusions: It can be concluded from this study that castor bean has high toxic and pathogenic effects that may be dangerous to the life of the organism. Therefore, it is advisable to be cautious of these pills and avoid exposure to them, also recommended to take high concentrations of doxycycline treatment when infected with castor bean poisoning.

CARDIOVASCULAR RISK FACTORS PECULIARITIES IN MEN UNDER 60 YEARS OLD WITH MYOCARDIAL INFARCTION AND CHRONIC INFLAMMATORY LUNG DISEASES

2020 ◽  
pp. 21-25
Author(s):  
Tupitsyn V.V. ◽  
Bataev Kh.M. ◽  
Men’shikova A.N. ◽  
Godina Z.N.
Keyword(s):  
Risk Factors ◽  
Myocardial Infarction ◽  
Heart Disease ◽  
Cardiovascular Risk ◽  
Digestive System ◽  
Control Group ◽  
Study Group ◽  
Type I ◽  
Internal Organs ◽  
History Of

Relevance. Information about the cardiovascular diseases risk factors (CVD RF) for in men with chronic lung inflam-matory pathology (CLID) is contradictory and requires clarification. Aim. To evaluate the peculiarities of CVD RF in men under 60 years of age with CLID in myocardial infarction (MI) to improve prevention. Material and methods. The study included men aged 19-60 years old with type I myocardial infarction. Patients are divided into two age-comparable groups: I - the study group, with CLID - 142 patients; II - control, without it - 424 patients. A comparative analysis of the frequency of observation of the main and additional cardiovascular risk fac-tors in groups was performed. Results. In patients of the study group, more often than in the control group we observed: hereditary burden of is-chemic heart disease (40.8 and 31.6%, respectively; p = 0.0461) and arterial hypertension (54.2 and 44.6%; p = 0.0461), frequent colds (24.6 and 12.0%; p = 0.0003), a history of extrasystoles (19.7 and 12.7%; p = 0.04); chronic foci of infections of internal organs (75.4 and 29.5%; p˂0.0001), non-ulcer lesions of the digestive system (26.1 and 14.6%; p = 0.007), smoking (95.1 and 66.3%; p˂0.0001), MI in winter (40.8 and 25.9%; p = 0.006). Less commonly were observed: oral cavity infections (9.2 and 23.6%; p˂0.0001); hypodynamia (74.5 and 82.5%; p = 0.0358), over-weight (44.4 and 55.2%; p = 0.0136), a subjective relationship between the worsening of the course of coronary heart disease and the season of the year (43.7 and 55.2%; p = 0.0173) and MI - in the autumn (14.1 and 21.9%; p = 0.006) period. Conclusions. The structure of CVD RF in men under 60 years of age with CLID with MI is characterized by the pre-dominance of smoking, non-ulcer pathology of the digestive system, frequent pro-student diseases, meteorological dependence, a history of cardiac arrhythmias and foci of internal organ infections. It is advisable to use the listed factors when planning preventive measures in such patients.

The effectiveness of use of enterosorbent Shelltic Es when rearing of young pigs

2020 ◽  
pp. 3-11
Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein
Keyword(s):  
Average Daily Gain ◽  
Live Weight ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Internal Organs ◽  
Feed Digestibility ◽  
Young Pigs ◽  
Carcinogenic Effects ◽  
Experimental Group

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.

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