scholarly journals Hydrogel Activity Test from Kirinyu Leaf Ethanol Extract (Chromolaenaodorata (L.) R. King & H. Rob) and Combination of Kirinyu Leaf Ethanol Extract with Collagen As A Healing Wound Excision.

2020 ◽  
Vol 8 (1) ◽  
pp. 35-37
Author(s):  
Thursina C S ◽  
Julia R ◽  
Sumaiyah S
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Activity Test ◽  
Collagen Group ◽  
Healing Wound ◽  
Wound Excision ◽  
Group 2 ◽  
Ethanol Extracts ◽  
Group 1

Objective: the purpose of this study was to determine the activity of the kirinyu leaf ethanol extract hydrogel preparation and the combination of the kirinyu leaf ethanol extract with collagen as a healing wound excision. Method: This research is experimental, where the ethanol extracts of Kirinyu leaves and collagen are formulated in the form of hydrogel preparations and tested on the healing of excision wounds in rats. Testing wound healing activities were divided into 6 groups. The Group 1 (negative control) were given hydrogel base without Kirinyu leaf ethanol extract in combination with collagen, group 2 (positive control) were given Octenilin®gel, groups 3 and 4, were given hydrogel of Kirinyu leaf ethanol extract while groups 5 and 6 were given hydrogel extract of kirinyu leaves combined with collagen. Result: The results of percent reduction in wound diameter showed that groups 3 and 4 gave significant differences compared to group 1 which began to be seen on the 10th day (p <0.05). While groups 5 and 6 gave significant differences that began to be seen on the 8th day (p <0.05). Conclusion:Hydrogel preparation in group 6 has the most potential in healing excision wounds.    

scholarly journals The Effectiveness of Sechium edule Jacq. Swartz Extract Changes in the Histopathology of Aspirin-Induced Rat Gastritis Model

2021 ◽  
Vol 9 (A) ◽  
pp. 1252-1257
Author(s):  
Hendrika Silitonga ◽  
Gontar A. Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan
Keyword(s):  
Treatment Group ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Infiltration ◽  
Group 5 ◽  
Group 2 ◽  
Ethanol Extracts

BACKGROUND: Gastritis is one of the most common diseases found in internal medicine clinics and daily life. AIM: The purpose of this study was to determine the histopathological changes of gastric rat model of aspirin-induced gastritis. MATERIALS AND METHODS: Group K(−) is a negative control (normal) not given any treatment, Group K(+) is a positive control induced by aspirin 200 mg/kgBW, Group 1 (P1) is a treatment group induced by aspirin 200 mg/kgBW with ethanol extract of chayote 100 mg/kgBW, po, Group 2 (P2) was the treatment group induced by aspirin 200 mg/kgBW with ethanol extract of chayote 200 mg/kgBW, po, Group 3 (P3) was the treatment group induced by aspirin 200 mg/kgBW with ethyl acetate fraction chayote 100 mg/kgBW, PO, Group 4 (P4) was the treatment group induced by aspirin 200 mg/kgBW with ethyl acetate fraction chayote 200 mg/kgBW, PO, and Group 5 (P5) was the treatment group induced by aspirin 200 mg/kgBW with omeprazole 20 mg. RESULTS: Analysis of the results of inflammatory infiltration in gastric histopathology in Groups P1, P2, P3, and P4 showed mild inflammatory infiltration compared to Groups P(5) and K(+). The description of the acinar glands on gastric histopathology showed that the P2 group gave a better picture of the acinar gland repair than the K(+), P2, P3, P4, and P5 groups. CONCLUSION: This study showed that ethanol extracts of squash 200 mg/KgBW own effectiveness as an anti-inflammatory on the improvement of infiltration of inflammatory and picture histopathological gastric mice compared with the ethanol extract of squash 100 mg/kg, the fraction of ethyl acetate chayote 100 mg/KgBW and 200 mg/KgBW and omeprazole 20 mg.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM)

2017 ◽  
Vol 6 (2) ◽  
pp. 125
Author(s):  
Rizkayanti Rizkayanti ◽  
Anang Wahid M. Diah ◽  
Minarni Rama Jura
Keyword(s):  
Vitamin C ◽  
Moringa Oleifera ◽  
Water Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ic50 Value ◽  
Antioxidant Potency ◽  
Ethanol Extracts ◽  
Moringa Oleifera Lam

Moringa (moringa oleifera Lam) leaves contains many molecules as inhibitors for free radicals such as phenolic compounds (phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, tannins), nitrogen compounds (alkaloids, amines, betalain), vitamins, terpenoids (including carotenoids), and several other endogenous metabolites as antioxidants. This study aimed to determine the antioxidant potency of water and ethanol extracts of moringa (moringa oleifera Lam) leave obtained by maceration and dekok. The concentration of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was analyzed using UV-Vis spectrophotometer after addition of various concentrations of Moringa leaves extracts. Various concentrations of moringa leave extracts used were 20 ppm, 40 ppm, 60 ppm and 80 ppm. Vitamin C solutions as the positive control were prepared on similar various concentrations. The negative control was prepared using DPPH solutions dissolved in absolute ethanol. The results indicated that the ethanol extract of moringa leaves prepared by maceration method showed the antioxidant potency with an IC50 value of 22.1818 ppm, but the IC50 value of water extract of moringa leaves prepared by dekok was 57.5439 ppm. While, the IC50 value of Vitamin C was 8.8084 ppm. Based on the IC50 data it can be concluded that Vitamin C is a stronger antioxidant than moringa leaves extracts.

Aktivitas Antibakteri dari Ekstrak Etanol Ganoderma sp. Asal Pulau Lombok

2020 ◽  
Vol 6 (1) ◽  
pp. 22
Author(s):  
Adelina Oktaviani ◽  
Aida Muspiah ◽  
Faturrahman Faturrahman
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Activity ◽  
Large Diameter ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Gram Negative Bacteria ◽  
Activity Test

The use of antibiotics that are not according to the rules and antibiotics in the long term can cause resistance to bacteria. This study aims to determine the presence of antibacterial activity and the effect of increasing the concentration of ethanol extract of Ganoderma sp. against several test bacteria. Extract from Ganoderma sp. obtained by maceration method using ethanol 95% solvent. The extract concentrations used were 20%, 40%, 60% and 80%. This research was conducted using the wells method with ciprofloxacin as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition formed around the well. The results of the antibacterial activity test of ethanol extract Ganoderma sp. has greater inhibitory activity against gram-negative bacteria. The inhibitory activity of ethanolic extract of Ganoderma sp. on the growth of test bacteria increased with increasing concentration of the extract

scholarly journals EFFICACY OF MOUTH RINSE FORMULATION BASED ON CETYLPYRIDINIUM CHLORIDE 0.1% IN THE CONTROL OF DENTAL CALCULUS BUILDUP

2017 ◽  
Vol 9 ◽  
pp. 176 ◽  
Author(s):  
Diah Ayu Maharani ◽  
Alia Ramadhani ◽  
Melissa Adiatman ◽  
Yuniardini Septorini Wimardhani ◽  
Linda Kusdhany ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Cetylpyridinium Chloride ◽  
Positive Control ◽  
Negative Control ◽  
Mouth Rinse ◽  
Dental Calculus ◽  
Double Blind ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Objective: This study aimed at comparing the antiplaque, anticalculus, and antigingivitis potentials of a mouth rinse containing essential oil, alcohol,zinc, and fluoride with a mouth rinse containing cetylpyridinium chloride (CPC) 0.1% over 1-, 2-, and 3-month periods.Methods: This study was a double-blind, parallel randomized clinical trial with a 3-day run-in phase. Respondents were asked to gargle twice dailywith 15 ml of mouth rinse for 30 seconds after brushing teeth. Respondents were 80 females with a mean age of 21 years, and a single dental examinerwas employed throughout the study to decrease the variance. Prophylaxis was performed for all respondents before the intervention. Three mouthrinses were tested: Group 1 with the mouth rinse containing CPC 0.1%, Group 2 as the negative control, and Group 3 as the positive control with amouth rinse containing alcohol. Evaluations were conducted by plaque index, gingival index, calculus index, and CariScreen examinations.Results: The clinical trial showed that the mouth rinse with alcohol and the mouth rinse containing CPC 0.1% were effective in inhibiting bacterialbuildup (antiplaque) and have anticalculus properties, but with no statistically significant antigingivitis effect.Conclusion: It was found that the mouth rinse containing alcohol has similar effectiveness with CPC 0.1% mouth rinse, but side effects, such as aburning sensation, were reported in the alcohol-containing mouth rinse.

scholarly journals PENGUJIAN EFEKTIVITAS DIURETIK KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) PADA TIKUS JANTAN

2020 ◽  
Vol 3 (1) ◽  
pp. 106-111
Author(s):  
Delisma Simorangkir
Keyword(s):  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
White Rats ◽  
Group 2 ◽  
Palm Leaf

African Leaf and palm leaf crops have a secondary metabolite content such as alkaloids and flavonoids that are efficacious as diuretics. The purpose of this study is to know the effectiveness of diuretics from the combination  African leaf ethanol extract and palm leaf ethanol extract. Methods used Eksperiemental. The test of diuretic activity is conducted divided into 6 treatment groups. Each group consists of 3 tails of white rats. Group 1 (positive control) administered Furosemide 3.6 mg/kgBB, Group 2 (negative control) was given suspension Na-CMC 0.5%, group 3 without treatment as well as groups of 4.5, and 6 in a row administered a combination of African leaf extract doses and extracts Oil palm leaf ethanol in a row of "50 mg/kgBB + 67, 5mg/kgBB"; "100mg/kgBB + 135 mg/kgBB"; and "200 mg/kgBB + 270 mg/kgBB". Each mouse is then given a 20ml NaCl/kgBB as an oral loading dose. Measured urine volume is recorded every hour for 6 hours after it continues to measure urine volume. The results showed that the combination of African leaf ethanol extract dosage and palm leaf ethanol extract had a diuretic effect if it was compared with negative control. The conclusion of the study was the results of the study showed that the combination of African leaf extract dosage and palm leaf extract is the most effective dose of Group 6 (African leaf extract 200 mg/kgBB + palm leaves 270 mg/kgBB ). Because it produces the highest urine volume of 23.01 ml.

scholarly journals Evaluation of Mutagenic and Antimutagenic Activity of Methanol Extract of Cousinia thomsonii against Cyclophosphamide

2020 ◽  
pp. 53-57
Author(s):  
Ishfaq Shafi Khan ◽  
Khalid Bashir ◽  
Naveed Gulzar ◽  
Yaseen Maqbool Bhat
Keyword(s):  
Bone Marrow Cells ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Free Access ◽  
Antimutagenic Activity ◽  
Male Wistar Rats ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

The present study was conducted to evaluate the mutagenic and antimutagenic potential of Cousinia thomsonii (CT) extract in bone marrow cells of male wistar rats using some important parameters like micronucleated polychromatic erythrocyte (MnPCE), mitotic index (MI), chromosomal aberrations (CA) and polychromatic erythrocyte to normochromatic erythrocyte ratio (PCE/NCE). 30 male rats of wistar strain were divided into 6 groups with 5 rats each group. Group 1 rats were taken as negative control having free access to distilled water and rat feed. Group 2 rats were taken as positive control treated with mutagen cyclophosphamide (CP) at dose of 60 mg/kg b wt. for 2 days. Group 3 and 4 were treated with CT extract at dose of 100 and 200 mg/kg b wt. for 20 days. Group 5 and 6 were treated with 100 and 200 mg/kg b wt of CT extract for first 18 days and for last 2 days with CP at dose concentration of 60 mg/kg. It was found that rats treated with CT extract alone did not produce any significant changes in MnPCE, PCE/NCE ratio, CA and MI when compared with control treated rats (group 1). However in group 5 and 6 rats treated with CT extract in combination with CP a protective effect was observed against the cyclophosphamide induced cellular mutagenicity. In concluding remark Cousinia thomsonii was found to show antigenotoxic potential and also produce protective antimutagenic effects against CP induced chromosomal damage.

scholarly journals Aktivitas Ekstrak Etanol Kulit Batang Kayu Jawa (Lannea coromandelica) terhadap Penyembuhan Luka Sayat pada Tikus Putih (Rattus Norvegicus L.)

2018 ◽  
Vol 4 (2) ◽  
pp. 113-118
Author(s):  
Umi Calsum ◽  
Akhmad Khumaidi ◽  
Khildah Khaerati
Keyword(s):  
Effective Dose ◽  
Rattus Norvegicus ◽  
Povidone Iodine ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Healing Time ◽  
White Rats ◽  
Lannea Coromandelica ◽  
Healing Wound

Jawa bark (Lannea coromandelica) is a part of the plant that can be used empirically for handling wounds. This study aims to determine the activity of ethanol extract of Jawa bark on healing wound and determine the effective dose compared with povidone iodine. The test animals used were white rats (Rattus norvegicus L.) consisting of 5 groups, namely negative control (vaseline without extract), positive control (povidone iodine), dose of 250 mg/kg BW, dose of 500 mg/kg BW, dose of 750 mg/kg BW. Each rat made an incision in the area parallel to the spine with 2 cm long and 2 mm deep. Measuring the length of the wound was done every day for 14 days. The data obtained were analyzed statistically using One Way Anova and followed by Post Hoc Duncan test. Statistical results showed that the ethanol extract of Jawa bark has an activity in curing slice where the effective dose is a dose of 500 mg/kg BW with a wound healing time of 12 days.

UJI POTENSI EKSTRAK DAUN BENALU BATU (BEGONIA SP) TERHADAP KADAR GLUKOSA DALAM DARAH DAN GAMBARAN HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (RATTUS NORVEGICUS)

2020 ◽  
Vol 6 (2) ◽  
pp. 286
Author(s):  
Joni Tandi ◽  
Dela Erni Paerunan ◽  
Nurifa Nurifa ◽  
Yunlis Silintowe Kenta ◽  
Sri Mulyani
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Glucose Levels ◽  
White Rats ◽  
Group I ◽  
Ethanol Extracts

This study aims to determine the potential for glucose levels in the blood of ethanol extracts of the begonias (Begonia Sp.), the effect of the extract on the pancreatic histopathology of white rats (Rattus norvegicus) which was induced by streptozotocin, and the effective dose begonias’ extract leaves on the histopathological descriptions of pancreatic male white rats. This study was an experimental laboratory where the research group consisted of group I (the control group) as a normal control was given a standard feed, group II (negative control) was given a 0.5% Na CMC suspension, group III (positive control) was orally given glibenclamide and experimental groups IV, V, and VI (doses 50, 100, and 200 mg/kg BW). Were given ethanol extract of begonias leaves. The results showed that the ethanol extract of the begonias (Begonia Sp.) contained secondary metabolite compounds, namely flavonoids, alkaloids, saponins, polyphenols, and tannins. Ethanol extract of begonias leaves at adose of 50 mg/kg BW was an affective dose in reducing blood glucoselevels by an avarage of 101,8 mg/dL) and a dose of 50 mg/kg BW can affect the histopathological picture of the pancreas in STZ induuced rats with an average damage value of 2 from a maximum of 4.

scholarly journals NEW MOLECULAR MECHANISM OF CEFTAZIDIME-INDUCED HUMAN RED BLOOD CELL HEMOLYSIS THROUGH THE PHOTOHEMOLYSIS REACTION

2018 ◽  
Vol 11 (15) ◽  
pp. 47
Author(s):  
Mashuri Mashuri ◽  
Achmad Zaini ◽  
Diana Rahmanisa ◽  
Muhammad Rifqi Alfiannoor ◽  
Muhammad Rosyhan Sanjaya ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Uv Light ◽  
Light Exposure ◽  
Positive Control ◽  
Negative Control ◽  
Group 3 ◽  
Group 2 ◽  
Experimental Group ◽  
Group 1

Objective: The present study was undertaken to investigate the photohemolysis reaction through photosensitization reaction by ceftazidime as a photosensitizer in human red blood cell (RBC).Methods: In this present study, human erythrocytes have used a sample. The sample then divided into six groups consisting of Group 1 (T1) served a negative control which consists of erythrocytes and buffers phosphate with pH 6.8; Group 2 (T2) served as a positive control which consists erythrocytes and buffers phosphate with pH 6.8 and exposed to UV-light; and Group 3, 4, 5, and 6 (T3, T4, T5, and T6) served as an experimental group which consists of erythrocytes, buffer phosphate with pH 6.8, ceftazidime with concentration 10%, 20%, 30%, and 40%, respectively, and also exposed to UV-light. UV-light exposure was done in 2 h. After the treatment period, the level of hydrogen peroxide (H2O2), conjugated diene (CD), advanced oxidation protein products (AOPPs), and percentage of RBC hemolysis (RBCH) were measured.Results: The results of this present studies showed that ceftazidime significantly increases the levels of H2O2, CD, AOPPs, and percentage of RBCH during the UV radiation.Conclusion: The present study demonstrated that ceftazidime acts as a photosensitizer and induced the photohemolysis reaction in human RBC. Furthermore, the hemolysis of RBC seems through the protein damage than lipid damage.

Delayed onset of paralysis and slowed tumor growth following in situ placement of recombinant human bone morphogenetic protein 2 within spine tumors in a rat model of metastatic breast cancer

2012 ◽  
Vol 16 (4) ◽  
pp. 365-372 ◽  
Author(s):  
Camilo A. Molina ◽  
Rachel Sarabia-Estrada ◽  
Ziya L. Gokaslan ◽  
Timothy F. Witham ◽  
Ali Bydon ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Positive Control ◽  
Negative Control ◽  
Control Groups ◽  
Spine Tumor ◽  
Experimental Animals ◽  
Bovine Collagen ◽  
Group 2 ◽  
Recombinant Human Bone ◽  
Group 1

ObjectRecombinant human bone morphogenetic proteins (rhBMPs) are FDA-approved for specific spinal fusion procedures, but their use is contraindicated in spine tumor resection beds because of an unclear interaction between tumor tissue and such growth factors. Interestingly, a number of studies have suggested that BMPs may slow the growth of adenocarcinomas in vitro, and these lesions represent the majority of bony spine tumors. In this study, the authors hypothesized that rhBMP-2 placed in an intraosseous spine tumor in the rat could suppress tumor and delay the onset of paresis in such animals.MethodsTwenty-six female nude athymic rats were randomized into an experimental group (Group 1) or a positive control group (Group 2). Group 1 (tumor + 15 μg rhBMP-2 sponge,13 rats) underwent transperitoneal exposure and implantation of breast adenocarcinoma (CRL-1666) into the L-6 spine segment, followed by the implantation of a bovine collagen sponge impregnated with 15 μg of rhBMP-2. Group 2 (tumor + 0.9% NaCl sponge, 13 rats) underwent transperitoneal exposure and tumor implantation in the lumbar spine but no local treatment with rhBMP-2. An additional 8 animals were randomized into 2 negative control groups (Groups 3 and 4). Group 3 (15 μg rhBMP-2 sponge, 4 rats) and Group 4 (0.9% NaCl sponge, 4 rats) underwent transperitoneal exposure of the lumbar spine along with the implantation of rhBMP-2– and saline-impregnated bovine collagen sponges, respectively. Neither of the negative control groups was implanted with tumor. The Basso-Beattie-Bresnahan (BBB) scale was used to monitor daily motor function regression and the time to paresis (BBB score ≤ 7).ResultsIn comparison with the positive control animals (Group 2), the experimental animals (Group 1) had statistically significant longer mean (25.8 ± 12.2 vs 13 ± 1.4 days, p ≤ 0.001) and median (20 vs 13 days) times to paresis. In addition, the median survival time was significantly longer in the experimental animals (20 vs 13.5 days, p ≤ 0.0001). Histopathological analysis demonstrated bone growth and tumor inhibition in the experimental animals, whereas bone destruction and cord compression were observed in the positive control animals. Neither of the negative control groups (Groups 3 and 4) demonstrated any evidence of neurological deterioration, morbidity, or cord compromise on either gross or histological analysis.ConclusionsThis study shows that the local administration of rhBMP-2 (15 μg, 10 μl of 1.5-mg/ml solution) in a rat spine tumor model of breast cancer not only fails to stimulate local tumor growth, but also decreases local tumor growth and delays the onset of paresis in rats. This preclinical experiment is the first to show that the local placement of rhBMP-2 in a spine tumor bed may slow tumor progression and delay associated neurological decline.

Sign in / Sign up

Export Citation Format

Share Document