Evaluation of Liver Protective Activity of Some Indigenous Plants Against Acute Paracetamol Toxicity in Rodents

Aim: The aim of the present study was to evaluate the liver protective activity of some indigenous plants against acute paracetamol toxicity in rodents. Liver intoxication was induced by paracetamol drug at a dose level of 500 mg/kg b.w, p.o for 9 days. To conduct this study the hydroalcholic extract of Prunus persica, Calotropis procera and Canscora decussate were taken as test compounds. Methods: Rats (180-200 g) were used for all the study and they were divided into 9 groups containing 6 animals each. Rats in Group I served as normal control (distilled water) group, Group II served as toxic control (Paracetamol treated) group, Group III served as standard (Silymarin) group. The rats of groups IV, V, VI, VII, VIII and IX served as test control groups. Group IV, V received the hydroalcoholic extract of Prunus persica at the dose of 200 and 400 mg/kg b.w, p.o respectively for 9 days. Group VI, VII received the hydroalcoholic extract of Calotropis procera at the dose of 200 and 400 mg/kg b.w, p.o respectively for 9 days. Group VIII and IX received the hydroalcoholic extract of Canscora decussate at the dose of 200 and 400 mg/kg b.w, p.o respectively for 9 days. The degree of protection was measured by using biochemical parameters such as serum glutamate oxalate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), bilirubin and total protein and albumin. Results: Results of this study showed that the treatment of the toxic effect of the paracetamol were significantly controlled in the hydroalcoholic extract of plants treated groups. The hydroalcholic extract of plants at 400 mg/kg b.w, showed significant reduction in elevated serum enzyme levels compared to paracetamol induced toxic group. The hydroalcoholic extract of Calotropis procera at a dose of 400 mg/kg, b.w showed the most significant hepatoprotective activity among all the test groups. Conclusion: From the results it was concluded that the all the test plants extract possess significant Hepatoprotective activity which was manifested by restoration of serum biochemical parameters to nearer the normal values. On the basis of results obtained, it can also be concluded that the hydroalcoholic extract of Plants seems to have hepatoprotective activity which may be due to the presence of flavonoids.

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AN EXPERIMENTAL STUDY TO EVALUATE THE HEPATOPROTECTIVE ACTIVITY OF NILITANDULIYADI LEHA IN PARACETAMOL INDUCED HEPATOTOXICITY IN WISTAR ALBINO RATS

Journal of Pharmaceutical and Scientific Innovation ◽

10.7897/2277-4572.105217 ◽

2021 ◽

Vol 10 (5) ◽

pp. 127-130

Author(s):

Sreekala Vijayan ◽

Jugal Kishore

Keyword(s):

Biochemical Parameters ◽

Wistar Rats ◽

Hepatoprotective Activity ◽

Albino Rats ◽

Test Drug ◽

Protective Activity ◽

Standard Drug ◽

Liver And Kidney ◽

Paracetamol Toxicity ◽

Wistar Albino Rats

Background: Paracetamol toxicity is currently the single most important cause for acute liver failure and is associated with significant number of deaths. Nilitanduliyadi leha is one among the formulation explained in the context of Vishahara yogas (anti-poisonous formulations) in the text Vishavaidya Jyotsnika. Objectives: To experimentally evaluate the hepatoprotective activity of nilitanduliyadi leha in paracetamol induced hepatotoxicity in Wistar albino rats. Methods: Albino Wistar rats of either sex weighing 200 – 250, g were selected and divided into four groups of six animals in each group (n = 6). Treatment was given for 7 days. Blood was drawn and sent for tests and important organs like liver and kidney were dissected out, cleaned to remove extraneous tissues, blotted to remove blood stain and weighed. A piece of liver tissue was preserved in 10% formalin for histopathological processing. Results: The formulation has helped in balancing the biochemical parameters studied almost as efficiently as the standard drug. In the antioxidant study also, the drug has given good results and shows even slightly more effective than the standard drug. The histopathology study also reveals mild protection and regeneration of tissues by the effect of test drug. Conclusion: This present study proves that the formulation is having a comparable hepato protective activity with that of silymarin.

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Antioxidant and protective effect of hydroalcoholic extract of Celtis australis L. on CCl4 induced hepatotoxicity

Pharmaceutical and Biomedical Research ◽

10.18502/pbr.v4i3.541 ◽

2019 ◽

Author(s):

Mohammad Shokrzadeh ◽

Hossein Bakhshi jouybari ◽

Majedeh Hosseinpour ◽

Ali Ziar ◽

Emran Habibi

Keyword(s):

Biochemical Parameters ◽

Radical Scavenging ◽

Hepatoprotective Activity ◽

Hepatic Tissue ◽

Serum Biochemical Parameters ◽

Destructive Effect ◽

Hydroalcoholic Extract ◽

Hepatic Diseases ◽

Serum Biochemical ◽

Celtis Australis

Several human diseases including cancer, rheumatoid arthritis, and neurodegenerative and hepatic diseases are related to the destructive effect of reactive oxygen species (ROS). Antioxidants may provide a possible solution to this problem. This study was carried out to investigate the effect of hydroalcoholic extract of Celtis australis on CCl4 induced hepatotoxicity in mice. The antioxidant activity of C. australis was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. For evaluation of hepatoprotective activity of the extract, the animals were pretreated with 200 and 400 mg/kg of C. australis extract intraperitoneally for 7 days and then received CCl4 (0.5 ml/kg in olive oil). Liver injury was determined by serum biochemical parameters such as Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), glutathione contents of liver tissue, and histopathological studies. A significant reduction in the serum biochemical parameters was observed when compared to the CCl4 receiving group. The standard antioxidant used in the study was ascorbic acid. C. australis extract significantly suppressed the increase in plasma activities of liver enzymes and effectively protected animals against CCl4-induced hepatic tissue damages. This study confirmed the hepatoprotective effect of the hydroalcoholic extract of C. australis.

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Hepatoprotective Activity of Some Indigenous Plants of Northern India Against Chronic Paracetamol Intoxication in Rats

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.2.6 ◽

2019 ◽

Vol 10 (02) ◽

Author(s):

Preeti Chaudhary ◽

Shamim , Ahmad ◽

Najam Ali Khan

Keyword(s):

Liver Injury ◽

Liver Toxicity ◽

Elevated Serum ◽

Hepatoprotective Activity ◽

Protective Role ◽

Calotropis Procera ◽

Indigenous Plants ◽

Major Health Problem ◽

Northern India ◽

Group I

Liver diseases have become a global concern worldwide. Liver injury or its dysfunction is a major health problem. The principal causative factors for liver injury are the availability of hepatotoxic drugs, alcohol consumption, infection, malnutrition, anemia etc. In the present study the hydroalcholic extract of some plants of northern India was evaluated for hepatoprotective activity against chronic paracetamol induced hepatotoxicity in rats. The liver injury was induced by Paracetamol orally at a dose of 500 mg/kg, b.w for entire duration of study. The rats were divided in nine groups. Rats of group I served normal control and received distilled water. Rats of group II served as toxic control and received Paracetamol orally at a dose of 500 mg/kg, b.w of rats. Group III received Silymarin at a dose of 100 mg/kg b.w, and served as standard. The animals of groups IV, V, VI, VII, VIII and IX served as test control groups. Protective effect of the hydroalcholic extract was assessed by measuring the levels of serum biomarkers such as SGOT, SGPT, ALP, Bilirubin, serum albumin and total protein. Results of this study showed that the treatment of the hydroalcholic extract of plants at 400 mg/kg b.w, showed significant (*** p less than 0.001) reduction in elevated serum enzyme levels compared to paracetamol induced toxic group, indicating the protective role of plants extract against paracetamol induced chronic liver toxicity. Among the all plants Calotropis procera at a dose of 400 mg/kg, b.w showed the most significant hepatoprotective activity.

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Evaluation of Hepatoprotective activity of Ethanolic Extract of Garuga pinnata Roxburgh leaves against Carbon tetrachloride induced Hepatotoxicity in rats.

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00419 ◽

2021 ◽

pp. 2375-2380

Author(s):

Sandeep Chavan ◽

Remeth Dias ◽

Chandrakant Magdum

Keyword(s):

Carbon Tetrachloride ◽

Biochemical Parameters ◽

Histopathological Examination ◽

Liquid Paraffin ◽

Ethanolic Extract ◽

Serum Levels ◽

Hepatoprotective Activity ◽

Antioxidant Defense Enzymes ◽

Serum Glutamate

In this study we investigated the in vivo Hepatoprotective activity of ethanolic extract of Garuga pinnata (EEGP) leaves in Carbon tetrachloride (CCl4) induced hepatotoxicity using wistar rats of either sex as model. Hepatotoxicity was induced by the administration of CCl4 intraperitoneally (0.125ml CCl4 in liquid paraffin (1:1) per 100g body weight). Garuga pinnata leaves extract at different dose levels (200 and 400mg/kg, p.o.) showed the dose dependant hepatoprotective effect and was compared with well known standard hepatoprotective Silymarain (100mg/kg). When groups were treated with CCl4, significant increase in serum biochemical parameters such as Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), Alkaline phosphate (ALP), Acid Phosphate (ACP), Creatinine and alteration of tissue biochemical parameters such as reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO), and the total proteins were observed. The histopathological examination of the CCl4 treated groups showed sinusoidal congestion, centrilobular necrosis, marked vacuolations and congestion. However, pretreatment with extract of leaves of Garuga pinnata significantly reduced the increased serum levels of biochemical parameters and restored antioxidant defense enzymes level to its normal. Moreover, histopathology of leaves extract treated groups showed normal architecture with minimal sinusoidal congestion. Taken together, our study concludes that EEGP to be a more potential agent for caring liver from CCl4 induced damage.

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Hepatoprotective Potential of Mimusops elengi L leaf Extracts against Paracetamol Induced Hepatotoxicity in Rats

International Journal of Pharmaceutical Sciences Review and Research ◽

10.47583/ijpsrr.2021.v69i02.005 ◽

2021 ◽

Vol 69 (2) ◽

Author(s):

B. Edwin Jose ◽

S. Manikandan ◽

S. Jebaseelan ◽

Dr.R. Meera ◽

Dr.R. Kalirajan

Keyword(s):

Aqueous Extract ◽

Biochemical Parameters ◽

Hepatoprotective Activity ◽

Leaf Extracts ◽

Standard Drug ◽

Herbal Plants ◽

Serum Glutamate Pyruvate Transaminase ◽

Glutamate Pyruvate ◽

Mimusops Elengi ◽

Serum Glutamate

Many traditional systems of medicines employ herbal drugs for the hepatoprotection. The aim of the study is to investigate the hepatoprotective activity of Mimusopselengi L leaf extracts extracts against paracetamol induced hepatotoxicity. However, herbal plants are the windfall for the humankind providing solution for most of the wellness breakdowns. Mimusopselengi L is one of such plants with enormous therapeutic and nutraceutical potencies. The main aspiration of the current investigation is to evaluate the hepatoprotective ability of methanolic and aqueous extract of Mimusopselengi L leaves against paracetamol induced hepatotoxicity using wistar rats through biochemical parameters and histopathological findings. The phytochemical screening was carried on the leaves extracts of Mimusopselengi L revealed the presence of some active ingredients such as Alkaloids, Tannins, Sponginess, Phenols, glycosides, steroids, terpenoids and flavonoids. Leaves of Mimusopselengi L was successively ethylacetate fraction with methanolic and aqueous extract against paracetamol (2 ml/kg.p.o) induced hepatotoxicity using Standard drug Liv 52 (5 ml/kg). There was a significant changes in biochemical parameters (increases in serum glutamate pyruvate transaminase (SGPT), Serum glutamate oxaloacetate transaminase (SGOT), alanine phosphatase (ALP), serum bilirubin in paracetamol treated rats, which were restored towards normalization in Mimusopselengi L methanolic and aqueous extract (200 mg/kg and 200 mg/kg) treated animals. Thus, the present study ascertains that the leaf extract of Mimusopselengi L possesses significant hepatoprotective activity.

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Hepatoprotective activity of Adina cordifolia against ethanol induce hepatotoxicity in rats

International Current Pharmaceutical Journal ◽

10.3329/icpj.v1i9.11619 ◽

2012 ◽

Vol 1 (9) ◽

pp. 279-284 ◽

Cited By ~ 5

Author(s):

A Sharma ◽

B Sangameswaran ◽

V Jain ◽

M S Saluja

Keyword(s):

Pharmaceutical Journal ◽

Hepatoprotective Activity ◽

Serum Markers ◽

Hepatocellular Injury ◽

Standard Drug ◽

Blood Samples ◽

Hepatic Cells ◽

Serum Glutamate Pyruvate Transaminase ◽

Histopathological Studies ◽

Serum Glutamate

The acetone (AEAC) and aqueous extracts (AQEAC) of Adina cordifolia, belonging to the family Rubiaceae, were studied for hepatoprotective activity against Wister rats with liver damage induced by ethanol. It was found that AEAC and AQEAC, at a dose of 500 mg/kg body weight exhibited hepatoprotective effect by lowering the Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT), alkaline phosphate and total bilirubin to a significant extent and also significantly increased the levels of total protein. The hepatoprotective activity was also supported by histopathological studies of liver tissue. Since results of biochemical studies of blood samples of ethanol treated rats showed significant increase in the levels of serum enzyme activities, reflecting the liver injury caused by ethanol and blood samples from the animals treated with AEAC and AQEAC showed significant decrease in the levels of serum markers, indicating the protection of hepatic cells against ethanol induced hepatocellular injury. The effects of AEAC and AQEAC were comparable with standard drug silymarin.DOI: http://dx.doi.org/10.3329/icpj.v1i9.11619 International Current Pharmaceutical Journal 2012, 1(9): 279-284

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Evaluation of Hepatoprotective and in vivo Antioxidant Potentials of Methanol Extract of Tephrosia pumila against Thioacetamide Induced Liver Toxicity in Wistar Rats

International Journal of Pharmaceutical Sciences Review and Research ◽

10.47583/ijpsrr.2021.v70i02.028 ◽

2021 ◽

Vol 70 (2) ◽

Author(s):

Ramesh C ◽

Pinkey Rawal ◽

Soma Pramanik ◽

Shabana S

Keyword(s):

Biochemical Parameters ◽

Methanol Extract ◽

Liver Toxicity ◽

Antioxidant Properties ◽

Total Duration ◽

Hepatoprotective Activity ◽

Oral Toxicity ◽

Standard Drug ◽

Protein Ions

The objective of the current investigation was performed to assess the hepatoprotective potentials and in vivo antioxidant properties of methanol extract of Tephrosia pumila against thioacetamide induced liver damage in rats. The acute oral toxicity study of methanol extract was determined as per OECD guidelines and the extract was proved to be safe up to the dose of 2000mg/kg. The total duration of the study was 21 days and animals were divided into six groups. Hepatotoxicity was induced in the animals of all groups except normal control by single dose administration of Thioacetamide(100mg/kg) at first day of the study followed by animals were treated daily with standard drug sylimarin and methanol extract of Tephrosia pumila (100mg/kg, 200mg/kg and 400mg/kg) to respective groups for 21 days. Variations in biochemical parameters like alanine transferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, albumin, total protein, ions and others parameters like clotting time and weight of the liver were considered to determine beneficial effect of the extract. At the end of the study liver samples were collected and subjected to histopathological evaluation. There were significant variations in the above mentioned biochemical parameters in toxic control animals treated with Thioacetamide alone while in the animals treated with methanol extract and standard drug silymarin, all the parameters were normal possibly due to their beneficial property in protecting the liver against thioacetamide induced hepatotoxicity. The results obtained in the above study suggesting that, the methanol extract of Tephrosia pumila possess significant hepatoprotective activity.

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EVALUATION OF ANTIPARKINSONIAN ACTIVITY OF HYDROALCOHOLIC EXTRACT OF THE SEEDS OF VIGNA ACONITIFOLIA IN WISTAR ALBINO RAT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i12.35693 ◽

2019 ◽

pp. 143-148

Author(s):

SUSHMITA SINGH ◽

IMTIYAZ ANSARI

Keyword(s):

Biochemical Parameters ◽

Motor Coordination ◽

Experimental Models ◽

Behavioral Changes ◽

Control Group ◽

Albino Rat ◽

Vigna Aconitifolia ◽

Hydroalcoholic Extract ◽

Treatment Groups ◽

Significant Difference

Objective: The objective of the study is to evaluate the antiparkinsonian activity of hydroalcoholic extract of the seeds of Vigna aconitifolia (HEVA) in Wistar albino rat. Methods: In rats, catalepsy was induced using haloperidol (4 mg/kg i.p.). Treatment groups received bromocriptine (4 mg/kg) and HEVA at the dose of (100, 200, and 300 mg/kg) orally. Bar test for catalepsy, motor coordination test by rotarod, and locomotor activity by actophotometer were carried out to assess behavioral changes. Assay of dopamine and catalase was also carried out to assess biochemical parameters. Results: Bromocriptine and HEVA-treated groups showed a significant difference in behavioral and biochemical parameters as compared to haloperidol control group in the experimental models. Conclusion: Vigna aconitifolia seeds exhibited significant antiparkinsonian activity in haloperidol mouse model.

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