scholarly journals Haematological profile in clinically suspected cases of neonatal Sepsis

2020 ◽  
Vol 11 (4) ◽  
pp. 5344-5349
Author(s):  
Rashmi L Sonawane ◽  
Mahendra A Patil ◽  
Sujata Kanetkar
Keyword(s):  
Blood Culture ◽  
Neonatal Sepsis ◽  
High Specificity ◽  
Clinical Suspicion ◽  
Sepsis Group ◽  
Klebsiella Pneumonia ◽  
Early Conclusion ◽  
Mortality And Morbidity ◽  
Haematological Profile ◽  
Wbc Count

Neonatal septicemia is one of the major factors contributing to the high perinatal and neonatal mortality and morbidity in newborns and is recognized as global health challenge. Study was aimed the changes in hematological profile along with blood culture and C-reactive proteins in clinically suspected cases of neonatal sepsis. The present study were included 108 neonates clinically suspicious to have sepsis and admitted in NICU. Avacutainer, Glass slides, Leishman’s stain, Automated Hematology analyzer, Staining Kit was used. Maximum number of neonates i.e. 80 (74%) were less than 2 days old. 70 (64.82%) were males and 38 (35.18%) were females. Maximum number (53.70%) were preterm, respiratory distress seen in (76.11%) Premature rupture of membranes was observed in 39 (36.11%). The clinical suspicion of sepsis, 24 (22.22%) had proven sepsis, . Rodwell’s hematological score of > 3 identified 23 out of 24 (95.83%) proven sepsis group neonates, 24 (88.89%) probable sepsis group neonates. Of the 108 neonates with clinical suspicion of sepsis, 24 (22.22%) had positive blood cultures. The most common pathogen isolated in the blood culture was Klebsiella pneumonia in 12 (50%). Leucocytosis was seen in 4 (16.6%). The total WBC count has low sensitivity (37%) but a high specificity (96%) as indicator of sepsis. Elevated immature PMN count was observed (79.2%) Elevated .Thrombocytopenia was noted in 9 cases (37.5%) proven sepsis. CONCLUSION- Rodwell’s hematological scoring framework is a straightforward, speedy, financially savvy instrument which can be utilized as screening test for early conclusion of neonatal sepsis.

scholarly journals A Study on Haematological Profile in Clinically Suspected Cases of Neonatal Sepsis

2020 ◽  
pp. 97-101
Author(s):  
S. R. Upadhey ◽  
Avinash M. Mane ◽  
Atul B. Hulwan ◽  
Rohit S. Kadam ◽  
Dhirajkumar Mane
Keyword(s):  
Blood Culture ◽  
Early Diagnosis ◽  
Observational Study ◽  
Neonatal Sepsis ◽  
Scoring System ◽  
Cost Effective ◽  
Clinical Findings ◽  
Culture Positivity ◽  
Blood Culture Positivity ◽  
Haematological Profile

The present study was a two year observational study of haematological profile in clinically suspected cases of neonatal sepsis. Study analyzed the haematological findings according to Rodwell’s haematological scoring system in neonates clinically suspicious of having sepsis. It included 108 neonates admitted in NICU of our hospital during the study period of June 2015 to May 2017. According to clinical findings, Rodwell’s hematological score and blood culture positivity 108 neonates were divided into three groups; proven sepsis, probable sepsis and no sepsis. Study show that Rodwell’s haematological scoring system is a simple, quick, cost effective tool which can be used as screening test for early diagnosis of neonatal sepsis.

scholarly journals The Relationship of Birth Weight Infant with Bacteriological Profile and Antibiotic Resistance of Neonatal Sepsis

2019 ◽  
Vol 7 (5) ◽  
Author(s):  
Nisrina Asysyifa ◽  
Husjain Djajaningrat ◽  
Diah Lestari
Keyword(s):  
Antibiotic Resistance ◽  
Birth Weight ◽  
Blood Culture ◽  
Low Birth Weight ◽  
Neonatal Sepsis ◽  
P Value ◽  
Klebsiella Pneumonia ◽  
Bacteriological Profile ◽  
Relationship Of ◽  
The Relationship

Neonatal sepsis is a major issue on neonatal-care field. This incident occurs by many factors, one of the factor is infant with a low birth weight. Blood culture is used as the gold standard for diagnosis. The spectrum of bacteria which caused neonatal sepsis is constantly change and vary due to antibiotic resistance phenomenon. This study aimed to determine the relationship of birth weight infant with bacteriological profile and antibiotic resistance of neonatal sepsis in RSAB Harapan Kita Jakarta. This study used observational method with cross sectional design and purposive sampling method which is analyzed by chisquare test. Medical record data and blood culture and antibiotic resistance test from all subjects in Januari until December 2018 were reviewed. The sample in this research is neonatal sepsis patients who met inclusion criteria were 51 patients. From 51 (55,43%) subjects, there were 39 (76,47%) neonatal sepsis in low birth weight infant. Klebsiella pneumonia spp (41,17%) and Staphylococcus epidermidis (19,60%) were the predominant pathogens. Amikacin (62,74%), and meropenem (50,98%) were the most susceptible antibiotic towards bacteria. Maximum resistance among organisms was seen in cefotaxime (84,31%), ceftazidime (78,43%), and amoxicillin (70,58%).  The result of the analysis found there is a relationship between birth weight infant and bacteriological profile (p-value = 0,035), but there is no relationship between birth weight infant and antibiotic resistance of neonatal sepsis (p-value =0,092; 0,066; and 0,521). There is a relationship between birth weight infant and bacteriological profile , but there is no relationship between birth weight infant and antibiotic resistance of neonatal sepsis.

scholarly journals Blood culture positive sepsis and sensitivity pattern in a tertiary care neonatal centre in eastern India

2019 ◽  
Vol 6 (2) ◽  
pp. 487
Author(s):  
Soumini Rath ◽  
Santosh Kumar Panda ◽  
Manas Kumar Nayak ◽  
Deepti Damayanty Pradhan
Keyword(s):  
Blood Culture ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Tertiary Care ◽  
Neonatal Care ◽  
Care Practice ◽  
Klebsiella Pneumonia ◽  
Gram Positive ◽  
Gram Negative ◽  
Culture Positive

Background: The objective of this study is to evaluate sensitive pattern of causative organisms of neonatal sepsis with its clinical outcome in a tertiary neonatal care unit of eastern India.Methods: This retrospective observational cohort study was done in a tertiary care hospital of Odisha. All the blood culture positive neonatal sepsis cases, excluding neonates with multiple congenital malformations, diagnosed during January 2017 to December 2018 were analysed using descriptive summary statistics.Results: A total of 73 neonatal sepsis cases were diagnosed by BacT/Alert and VITEK-2 blood culture method. Among them, 50 (68%) babies had gram negative sepsis,14 (19%) cases of gram-positive sepsis and nine (13%) cases of fungal sepsis. In present study 38 (52%) cases were early onset sepsis, 38(52 %) babies were term, 55(75%) were male and 44 (60%) babies were out born. Klebsiella pneumonia and Acinetobacter were the most common organism in early onset and late onset sepsis respectively. Among gram negative organism (GNB), 66% were multi drug resistant. A fifty percent of gram-negative organism were sensitive to meropenem and 28% were sensitive to piperacillin/tazobactam. The sensitivity of GNB to colistin, ciprofloxacin, amikacin was 76%, 64% and 56% respectively. The sensitivity of gram-positive organisms to linezolid, vancomycin, teicoplanin and penicillin were 92%, 85%,85% and 20% respectively. Survival rate among culture positive sepsis was 83%.Conclusions: Multi drug organisms are emerging in modern neonatal care practice. Practice of antibiotic stewardship may save the babies from multidrug resistance organism in future.

scholarly journals Diagnostic Significance of White Blood Cell Count and C-Reactive Protein in Neonatal Sepsis; Asella Referral Hospital, South East Ethiopia

2018 ◽  
Vol 12 (1) ◽  
pp. 209-217
Author(s):  
Abebe Sorsa
Keyword(s):  
Blood Culture ◽  
Blood Cell ◽  
White Blood Cell ◽  
Neonatal Sepsis ◽  
C Reactive Protein ◽  
Diagnostic Significance ◽  
Gram Negative ◽  
Reactive Protein ◽  
Wbc Count ◽  
Sensitivity Specificity

Introduction:Nowadays various biochemical markers, such as C-Reactive Protein (CRP), Procalcitonin and tumor necrosis factor alpha, have been proposed as a potential marker for screening neonatal sepsis. In the current study, we tried to see the diagnostic significance of White Blood Cell (WBC) count and CRP in diagnostic screening of neonatal sepsis.Methods:A prospective cross-sectional study was conducted from May 2016 to April 2017 in Asella Teaching and Referral Hospital. Data were entered into EPI-INFO version 3.5.1 for cleanup and then exported to SPSS version 17 for further analysis. Sensitivity, specificity, positive predictive value (PPV) and negative predictive values (NPV) were used to assess the accuracy of CRP and WBC count taking blood culture as gold standard.Results:Data of 303 neonates with clinical sepsis were analyzed. Positive CRP and abnormal WBC were reported in 136(45%) and 99(32.7%) of study subjects respectively. Blood culture turned to be positive in 88(29.4%) of study subjects. The Sensitivity, Specificity, PPV and NPV of WBC count were 59.5 %, 79.6%, 52%, 64.5% respectively while the sensitivity, specificity, PPV and NPV of CRP were 65.6%, 78%, 42% and 91% respectively. By combining both WBC and CRP, the sensitivity, specificity, PPV and NPV improve to 78.5%, 83%, 60% and 93% respectively. CRP positivity rate was comparable across gram positive and gram negative bacteria while high WBC count were more reported among gram positive sepsis than gram negative ( OR 4.8, (95% CI 1.45-15.87, P 0.01)Conclusion:Based on this study’s finding, it can be concluded that CRP alone or in combination with WBC count showed better diagnostic accuracy in neonatal sepsis.

scholarly journals Comparative Study of Diagnostic Markers in Neonatal Sepsis

2014 ◽  
Vol 34 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Vijay Baburao Sonawane ◽  
Sonali U Gaikwad ◽  
Nitin N Kadam ◽  
Jitendra Gavhane
Keyword(s):  
Risk Factors ◽  
Blood Culture ◽  
Comparative Study ◽  
Neonatal Sepsis ◽  
Signs And Symptoms ◽  
Diagnostic Markers ◽  
Control Group ◽  
Specific Marker ◽  
Study Group ◽  
Mortality And Morbidity

Introduction: Neonatal septicemia is one of the commonest causes of neonatal mortality and morbidity. The objectives of this study were intended for qualitative and quantitative analysis of diagnostic markers in neonatal sepsis.Materials and Methods: This is a hospital based study conducted over three years (Aug 2005 – Aug 2008). Hundred and sixty neonates, delivered in the hospital, having risk factors for neonatal sepsis, along with those coming to hospital with signs and symptoms of sepsis up to 28 days of life (as study group ) along with normal newborns admitted to the postnatal ward without high risk factors (control group) were enrolled for this study. Comparative study on various diagnostics markers such blood culture, CBC,CRP, IT ratio and Micro-ESR was carried out to know their sensitivity and specificity.Results: E.Coli was the most common organism responsible for sepsis. CRP was reported to be highly sensitive (84.21%), and CBC was highly specific (75.00%), IT ratio has sensitivity of 62.5% and specificity of 56.25% while Micro-ESR has shown sensitivity of 50.0% and specificity of 62.5%,Out of 160 cases, blood culture (BacTalert) showed growth in48 cases in study group while two cases in control group. Thus blood culture positivity was 60%.Conclusion: Blood culture is the gold standard for the diagnosis of septicemia. CRP is most sensitive while CBC is most specific marker in neonatal sepsis.DOI: http://dx.doi.org/10.3126/jnps.v34i2.9788J Nepal Paediatr Soc 2014;34(2):111-114  

scholarly journals Comparison between sepsis markers and blood culture in diagnosis of neonatal sepsis: a prospective study

2017 ◽  
Vol 5 (4) ◽  
pp. 1662
Author(s):  
Vijay Baburao Sonawane ◽  
Nitin Mehkarkar ◽  
Sonali Gaikwad ◽  
Nitin Kadam
Keyword(s):  
Blood Culture ◽  
Neonatal Sepsis ◽  
Gold Standard ◽  
Predictive Accuracy ◽  
Statistical Correlation ◽  
Diagnostic Process ◽  
Mortality And Morbidity ◽  
Sepsis Screen ◽  
A Prospective Study ◽  
Sepsis Detection

Background: Neonatal septicaemia is one of the commonest causes of neonatal mortality and morbidity. Accurate and timely diagnosis of neonatal sepsis remains a major challenge to the pediatricians and neonatologists. In the present study, correlation between sepsis screening and blood culture in neonate presenting with features of sepsis is done to accelerate the diagnostic process and blood culture (considered gold standard) was evaluated as marker for sepsis detection and its effectiveness was compared with other septic markers.Methods: In present study, we emphasize to study early indicators of sepsis screen and their statistical correlation with blood culture (considered as gold standard).Results: As any sepsis screen parameters showed little correlation with blood culture, yet on combination it was found that specificity and positive predictive accuracy increased while sensitivity decreased them individual tests. Also combination of tests yield better results than single tests.Conclusions: The combination of sepsis makers yielded diagnostic results than single tests and proved to be an invaluable aid for early diagnosis of neonatal sepsis.

scholarly journals Role of Haematological Score in Early and Rapid Diagnosis of Neonatal Septicaemia

2017 ◽  
Vol 7 (3) ◽  
pp. 212-215
Author(s):  
Shareen Khan ◽  
MA Baki ◽  
Amrita Lal Halder ◽  
Afroza Haque ◽  
Tahmina Begum ◽  
...  
Keyword(s):  
Blood Culture ◽  
Scoring System ◽  
Positive Culture ◽  
Neonatal Morbidity ◽  
Rapid Diagnosis ◽  
Mortality And Morbidity ◽  
Neonatal Septicaemia ◽  
Wbc Count ◽  
The Right

Background: Septicaemia is well documented as being one of the major causes of neonatal mortality and morbidity worldwide including Bangladesh. A haematological scoring system which includes total WBC count, absolute neutrophil count, platelet count and C reactive protein may be beneficial for the early and rapid diagnosis of neonatal septicaemia. So this study was done to see the role of the haematological score in neonatal septicaemia.Methods: This observational study was done in SCABU, BIRDEM General Hospital 2 from April 2012 to September 2012. One twenty neonates who were clinically suspected of septicaemia were enrolled in this study. A score of 1 was given for each of the haematological parameters. Blood culture was done in all neonates along with haematological score to prove septicaemia.Results: In this study 42 neonates had a score of 1,a score of 2 was present in 45 neonates. Score 3 was present in 23 neonates and score 4 was present in 10 patients. Among the 42 neonates having a score of 1, culture was negative in 69% and 30% had positive culture reports. In neonates with score 2, it was seen that almost half (46.4%) had a positive blood culture and the rest (53.3%) were culture negative. Twenty three neonates had a score of 3 among which 73.9 % were proven sepsis by positive culture reports. Blood culture was positive in 100 % patients with a score of 4.Conclusion: The higher the score the greater the chance of neonatal septicaemia. The haematological scoring system may aid the clinicians to use proper antibiotics at the right time and thus reduce neonatal morbidity and mortality.Birdem Med J 2017; 7(3): 212-215

scholarly journals Evaluation of screening of neonatal sepsis

2018 ◽  
Vol 5 (2) ◽  
pp. 580
Author(s):  
Kartik R. ◽  
Sahana Manjunath ◽  
Prathiba Doddabasappa ◽  
Malavika J.
Keyword(s):  
Blood Culture ◽  
Early Diagnosis ◽  
Sensitivity And Specificity ◽  
Neonatal Sepsis ◽  
Maximum Sensitivity ◽  
Total Leucocyte Count ◽  
Mortality And Morbidity ◽  
Protein Assay ◽  
Sepsis Screen ◽  
Culture Negative

Background: Neonatal sepsis is an important cause of neonatal mortality and morbidity. Early diagnosis of sepsis is difficult due to its non- specific clinical presentation. The gold standard for diagnosis is blood culture, which is obtained in only 25%-40% of cases and requires 48-72 hours. There is a need for a sepsis screen for early diagnosis of septicemia and identification of culture negative cases. The objective of the study was to study the role of sepsis for early diagnosis of septicaemia and identification of culture negative cases and to compare the rapid diagnostic tests with blood culture singly and in combinations for specificity and sensitivity.Methods: 60 cases of suspected septicemia were studied. Total leucocyte count, bandforms peripheral smear examination, C-reactive protein assay, micro-ESR, and blood culture study was investigated. Results: Study revealed that CRP had maximum sensitivity while band neutrophil ratio had balanced sensitivity and specificity. In the two tests, CRP with PS/BF had balanced sensitivity and specificity. In the three tests combination, CRP with TC with micro-ESR had balanced sensitivity and specificity in proven sepsis, While CRP with BF with micro-ESR had balanced sensitivity and specificity in most probable sepsis cases.Conclusions: Neonatal sepsis has vague signs and symptoms, so high index of suspicion helps in arriving early diagnosis and management. CRP had maximum sensitivity in the individual tests. Using either two tests (CRP + PS/BF) or three tests (CRP + Micro ESR + BF/TC) most of the sepsis cases could be identified and sepsis negative cases can be ruled out. Sepsis screen is helpful in avoiding overuse of antibiotics.

scholarly journals A study on bacteriological profile, drug sensitivity and resistance pattern of isolated organism in neonatal septicaemia in neonatal intensive care unit

2017 ◽  
Vol 4 (4) ◽  
pp. 1430
Author(s):  
Himanshu A. Joshi ◽  
Seema S. Shah
Keyword(s):  
Blood Culture ◽  
Klebsiella Pneumoniae ◽  
Neonatal Sepsis ◽  
Neonatal Intensive Care ◽  
Bacterial Pathogen ◽  
Empirical Therapy ◽  
Resistance Pattern ◽  
Mortality And Morbidity ◽  
Medical College ◽  
Bacteriological Profile

Background: Neonatal Sepsis is one of the major cause of mortality and morbidity in Neonates. Bacterial pathogens and drug resistance varies from different hospitals areas, regions and countries. Advances in early diagnosis and treatment have led to better prognosis of newborns in NICU. This study will provide the bacterial pathogen causing neonatal sepsis along with their antibiogram. The pattern of susceptibility to antibiotics in NICU at GMERS Medical College centre, was very helpful to start the empirical therapy.Methods: This retrospective study was carried out in GMERS Medical College, Gandhinagar from February 2015 to January 2016. Period in 228 neonates. Out of which 131 cases were positive for blood culture. The positive blood culture was detected by Bactec blood culture system.Results: In present study 131 out of 228 cases were culture positive (57.48%). In gram negative organism Klebsiella pneumoniae was the commonest followed by E. coli (3.05%) and Pseudomonas (2.3%) CONS was the most common isolates in from the group. Klebsiella pneumoniae was sensitive to levofloxacin (9816), imipenem (88.15%) and piperacillin+tazobactam (88.15%). This organism was having less sensitivity to routine 1st line antibiotics like ampicillin (9.2%), gentamycin (27.4%), amikeine (35.5%) amoxy+clavulinic (21%) acid, cefotaxime (25%), gram positive CONS having sensitivity to vanceomycin (8.8%), amkacine, ampicillin, levofloxacin, cefotaxime were having limited effect on CONS organism.Conclusions: There is an increasing trend of antibiotic resistance to the commonly used first line drugs. The pattern of sensitivity is changing hence continuous survelliance for antibiotic susceptibility is needed to ensure correct empirical therapy before blood culture reports are available. 

scholarly journals Clinical features and septic screen in neonatal sepsis in a teaching hospital in India

2019 ◽  
Vol 7 (1) ◽  
pp. 46
Author(s):  
Soja Vijayan ◽  
Gopalan A. Velayudhan Nair ◽  
Dhanya Narayanan
Keyword(s):  
Blood Culture ◽  
Clinical Features ◽  
Teaching Hospital ◽  
Neonatal Sepsis ◽  
Coagulase Negative Staphylococcus ◽  
Coagulase Negative Staphylococci ◽  
Cross Sectional ◽  
Mortality And Morbidity ◽  
Live Births ◽  
Culture Positive

Background: Sepsis remains a leading cause of mortality and morbidity, especially during the first five days of life and in low and middle-income countries. The purpose of this study was to note the clinical features and analyze the relationship between the septic screen and blood culture positive sepsis in the neonatal unit.Methods: A one-year descriptive cross-sectional study was carried out at the NICU of a teaching hospital in India.Results: The incidence of clinically suspected septicemia was 19.3 per 1000 live births and the incidence of blood culture positive septicemia was 2.9 per 1000 live births among the inborn of the hospital. The most common clinical features were poor suck and lethargy in culture positive sepsis. The most common organisms causing sepsis were Coagulase negative staphylococci and Klebsiella. In the septic screen CRP was found to have a statistically significant association with blood culture positive sepsis. CRP also had the highest sensitivity and negative predictive value among the studied parameters.Conclusions: Incidence of blood culture positive sepsis was 2.9 per 1000 live births among the inborn of the hospital. The most common clinical features were poor suck and lethargy in culture positive sepsis. The most common organism isolated in neonatal sepsis in the NICU was Coagulase negative staphylococcus. In resource poor settings, CRP continues to be an important tool in diagnosis and treatment of neonatal sepsis.

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