scholarly journals Comparison of laparotomic omentopexy vs. laparoscopic abomasopexy treatments of left displaced abomasum in dairy cows under field conditions: biochemical analysis

2020 ◽  
Vol 89 (3) ◽  
pp. 209-216
Author(s):  
Tomáš Haloun ◽  
Radko Rajmon ◽  
Dalibor Řehák ◽  
Helena Hartlová ◽  
Zuzana Ptáčková ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
Monitoring Program ◽  
Blood Parameters ◽  
Field Conditions ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Biochemical Profile ◽  
Calcium Magnesium ◽  
Haptoglobin Concentration ◽  
The Difference

The objective of this retrospective study was to compare the effects of the two methods (laparoscopic or laparotomic) of LDA (left displaced abomasum) correction under field conditions by means of survival rate and biochemical profile evaluation. Holstein cows from one farm with LDA over a 20-month period were included in the study. Cows underwent laparoscopic abomasopexy (Janowitz’s method, LS) or a laparotomic right flank omentopexy (Dirksen’s method, LT). Blood samples for biochemical analysis were taken just prior to surgery (D1) and on days 10 (D10) and 30 (D30) following surgery. Blood profiles of healthy cows identified from the farm’s routine transition cow monitoring program were used as control (C). Aiming to minimize disruptive effects of quite a long period of data collection, the cows’ biochemical profile was evaluated in three orthogonal comparisons: LS cows vs. control group, LT cows vs. control group, and LS cows vs. LT cows. The rate of animal survival was similar for both methods (loss of about 11% until 30 days after treatment). Most of the blood parameters from LS and LT groups were comparable in all three sampling terms indicating continual organism recovery. At D10 the difference of higher cholesterol concentration and lower haptoglobin concentration were favourable for the LS group (P < 0.05). Total protein, calcium, magnesium and albumin showed more positive dynamics in the LS group too. This proves previous laboratory and clinical indices of expediency of LDA laparoscopy treatment under field conditions.

scholarly journals Effect of short-chain fructooligosaccharide-enriched energy-restricted diet on weight loss and serum haptoglobin concentration in Beagle dogs

2011 ◽  
Vol 106 (S1) ◽  
pp. S120-S123 ◽  
Author(s):  
Rebecca Ricci ◽  
Isabelle Jeusette ◽  
Jean-Marie Godeau ◽  
Barbara Contiero ◽  
Marianne Diez
Keyword(s):  
Weight Loss ◽  
Test Group ◽  
Blood Parameters ◽  
Short Chain ◽  
Control Group ◽  
Beagle Dogs ◽  
Restricted Diet ◽  
Serum Haptoglobin ◽  
Before And After ◽  
Haptoglobin Concentration

The effects of the dietary inclusion of two levels of short-chain fructooligosaccharides (sc-FOS) on weight loss, biochemical parameters and serum haptoglobin concentration were investigated in twelve experimental obese Beagle dogs. Dogs were randomised into two groups and submitted to a weight loss program (WLP): the control group (C) received a commercial energy-restricted high-protein diet containing 1 % DM sc-FOS, whereas the test group (T) received the same diet enriched with sc-FOS to attain a 3 % DM content. Body weight (BW) and body condition score were weekly assessed in each dog and blood was collected before and after WLP to measure total plasma cholesterol (CHOL), TAG, NEFA, glucose (GLUC), insulin, serum leptin and haptoglobin. Groups showed similar BW and blood parameters before treatment. When values before and after treatment of the dogs were compared, significant reductions were observed for all parameters, with the exception of NEFA and GLUC. However, when these reductions were compared between C and T groups, significant differences were detected only for haptoglobin (T before v. T after: 1545 v. 605 mg/l, P = 0·03; C before v. C after: 1635 v. 1400 mg/l, P = NS). Positive correlations between haptoglobin and CHOL and between haptoglobin and TAG were observed before but not after WLP. In conclusion, feeding obese dogs with the energy-restricted diet caused significant weight loss and reduction of blood parameters, irrespective of the sc-FOS content included. However, serum haptoglobin level, and the subclinical inflammatory condition associated with it, was significantly lowered in the T but not in the C group.

scholarly journals Biochemical and haematological parameters in dogs with Cushing’s syndrome

2021 ◽  
Vol 52 (4) ◽  
Author(s):  
Ernes Karić ◽  
Aida Hodžić ◽  
Amir Zahirović ◽  
Amina Hrković-Porobija ◽  
Husein Orhan
Keyword(s):  
Cushing’S Syndrome ◽  
Clinical Signs ◽  
Blood Parameters ◽  
Cushing's Syndrome ◽  
The Body ◽  
Haematological Parameters ◽  
Control Group ◽  
Blood Concentrations ◽  
The Difference ◽  
As Stress

The exposure of the body to stress, regardless of whether it comes from physical, chemical or emotional stimuli from the environment, causes an inadequate adaptation of the organisms which can contribute to the development of various diseases. Abnormally high blood concentrations of cortisol, known as stress hormone, lead to the development of a hormonal disorder called hyperadrenocorticism or Cushing’s syndrome. In the majority of cases, Cushing’s syndrome is diagnosed when symptoms are apparent, and screening endocrinological test confirms the existence either of increased cortisol production or decreased sensitivity of the hypothalamic-pituitary-adrenal axis to negative glucocorticoid feedback. In our research, we examined a total of 23 male and 7 female dogs that were suspected to have Cushing’s syndrome, based on history and clinical signs. A total of 15 male and 5 female dogs were positive for Cushing’s syndrome (HAC group), whereas the remaining dogs were used to form non-HAC group. Using the apparatus IDEXX “Vet Test 8008”, the following biochemical parameters were determined: glucose, urea, creatinine, phosphorus, calcium, total protein, albumin, globulin, alanine aminotransferase, alkaline phosphatase, bilirubin, cholesterol, and amylase. Regarding haematological parameters, the following parameters were investigated: erythrocytes, leukocytes, platelets, erythrocyte indices (MCV, MCH, MCHC, RDW), white blood cell count, haemoglobin and haematocrit, using “Laser cite vet lab Station” (IDEXX). No significant differences in haematological and biochemical blood parameters were noticed between the HAC and the non-HAC group of dogs. However, dogs suffering from Cushing’s syndrome had a higher value in the number of erythrocytes compared to the control group. The finding that has to be payed attention to is the difference in platelet count between the control group of dogs and dogs suffering from Cushing’s syndrome.

scholarly journals The Ability of Bile to Scavenge Superoxide Radicals and Pigment Gallstone Formation in Guinea Pigs

HPB Surgery ◽  
1996 ◽  
Vol 10 (2) ◽  
pp. 73-77 ◽  
Author(s):  
Cong Lin ◽  
Tao Shen ◽  
Xianbo Fu ◽  
Xiaosi Zhou
Keyword(s):  
Bile Duct ◽  
Guinea Pigs ◽  
Biochemical Analysis ◽  
Gallstone Formation ◽  
Control Group ◽  
Exact Probability ◽  
Pigment Gallstones ◽  
Pigment Gallstone ◽  
The Common ◽  
The Difference

After partial ligation of the common bile duct (CBD) of guinea pigs, 14 of 16 animals developed pigment gallstones within one week (S group). Intraperitoneal injection of Vit. E and C, each 10 mg/kg daily from 3 days before CBD ligation to one week after the operation (S+V group), decreased the gallstone incidence to 5/14 (exact probability<0.01). The gallstone incidence in the control group, that only received laparotomy without ligation of the CBD, was 0/15. Biochemical analysis of the gallbladder bile showed that stricture of the CBD was associated with a significant increase in levels of unconjugated bilirubin (UCB) and Ca2+ (p<0.05 and <0.01). Simultaneously the scavenging rate (SR) of superoxide radical in bile significantly decreased (p<0.05). Comparing S+V group with S group, the effect of Vit. E and C on the concentrations of UCB and Ca2+ in bile was not significant (both p>0.05), but Vit. E and C normalized the SR, and the difference between S group and S+V group was significant (p<0.05). These results suggested that Vit. E and C, known as antioxidants, enhanced the ability to scavenge oxygen radical in S+V group; and that in addition to the increases of UCB and Ca2+ concentrations, the participation of oxygen radicals might be of importance for pigment gallstone formation induced by bile duct obstruction.

scholarly journals Biochemical profile of Mangalarga Marchador mares during the last third of pregnancy, foaling and lactation

2019 ◽  
Vol 49 (11) ◽  
Author(s):  
Micheline Ozana da Silva ◽  
José Dantas Ribeiro Filho ◽  
Waleska de Melo Ferreira Dantas ◽  
Bruna Maria Ribeiro ◽  
Pedro Ancelmo Nunes Ermita ◽  
...  
Keyword(s):  
Uric Acid ◽  
Biochemical Analysis ◽  
Major Influence ◽  
Biochemical Profile ◽  
Total Calcium ◽  
Sodium Potassium ◽  
Physiological Conditions ◽  
Biochemical Constituents ◽  
Calcium Magnesium ◽  
Experimental Group

ABSTRACT: Adaptive changes in metabolism may occur under specific physiological conditions, being necessary the knowledge of the biochemical constituents that can undergo variations during the sixty days before foaling, until the first sixty days of lactation. The present study aimed to identify and evaluate the biochemical profile of Mangalarga Marchador mares during the last third of pregnancy, foaling and lactation. For this, 48 mares were used, which formed a maintenance group (MG), composed of non-pregnant mares and an experimental group, called the transition group (TG), formed by pregnant mares. Blood samples were collected at the following times: MG was evaluated in a single time (beginning of all collections); TG was evaluated in seven times [T-60, T-30, T-15 (days before foaling), T0 (first six hours after foaling), T15, T30, T60 (days after foaling)]. All animals were submitted to biochemical analysis, in which the concentrations of total calcium, chloride, phosphorus, magnesium, sodium, potassium, uric acid, creatinine, and urea were measured. The two groups differed significantly (P<0.05) in calcium, magnesium, sodium, potassium, creatinine, uric acid and urea concentration. Variation between times was also observed for these compounds. However, chloride and phosphorus did not differ between MG and TG. It was concluded that the last third of pregnancy, foaling and lactation exerts major influence on the biochemical constituents of blood through adjustments to maintain homeostasis in mares.

scholarly journals Propiscin — A safe anaesthetic for pikeperch (Sander Lucioperca L.)

2016 ◽  
Vol 64 (4) ◽  
pp. 415-424 ◽  
Author(s):  
Maciej Rożyński ◽  
Elżbieta Ziomek ◽  
Krystyna Demska-Zakęś ◽  
Agata Kowalska ◽  
Zdzisław Zakęś
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Control Group ◽  
Sander Lucioperca ◽  
Mean Values ◽  
Recirculating Aquaculture ◽  
Calcium Magnesium ◽  
The Mean ◽  
The Impact

The aim of this work was to determine the impact of etomidate (Propiscin) dose (1 and 2 ml l−1) and exposure time (2 and 10 min) on the biochemical and haematological parameters of juvenile pikeperch (Sander lucioperca) [mean body length (Lc) 25.9 cm; body weight (W) 189.9 g] that were reared in a recirculating aquaculture system (RAS). Significant changes in the mean values of total protein, globulin, calcium, magnesium, and ammonia were noted in all groups immediately following exposure. The greatest changes in the haematological indicators were observed in groups subjected to 10-min exposure at both doses of the anaesthetic. The specimens from these groups had higher values for white blood cells (WBC), red blood cells (RBC), haemoglobin (HGB), haematocrit (HCT), and mean corpuscular volume (MCV). Statistically significant differences in these same parameters were also noted in the groups of fish exposed to the anaesthetic for 2 min at a dose of 2 ml l−1, but they were not as pronounced. Twenty-four h following exposure to etomidate, all blood parameters in the experimental groups were comparable to those of the control group. Etomidate can be recommended as a safe, effective anaesthetic for pikeperch.

scholarly journals Combined Effect of Monieziosis and Hypomicroelementosis on Some Hematological, Biochemical and Hormonal Parameters in Merino Sheep

2021 ◽  
Vol 41 (01) ◽  
pp. 107-111
Author(s):  
Mahmoud Abdelhamid
Keyword(s):  
Biochemical Analysis ◽  
Blood Parameters ◽  
Control Group ◽  
Merino Sheep ◽  
Internal Parasites ◽  
Serum T4 ◽  
The Impact ◽  
Experimental Group ◽  
Hormonal Analysis ◽  
Apparently Healthy

This study was done to determine the impact of monieziosis infection combined with hypomicroelementosis on some hematological, biochemical and hormonal parameters of Soviet Merino sheep in the Astrakhan region. 20 sheep, aging 3 years old and average 43±1.6 kg body weight were used. Sheep were divided into two groups. First group contained 10 sheep and these sheep were naturally infected with monieziosis and clinically were suffered from hypomicroelementosis, emaciation, reduced growth rate, anemia, diarrhea and pale mucosa. Second group contained 10 sheep were apparently healthy and free from internal parasites and they were used as a control group. The first group was given Praziver (praziquantel and ivermectin) for treatment monieziosis, while they intramuscularly injected with Sedimin (selenium, iodine and iron) and were introduced daily into the feed with CoCl2. Faecal and blood samples from both groups were collected, before and 30 days after treatment, and analyzed for some hematological, biochemical and hormonal parameters. Our results revealed that there was a significant decrease in Hb and RBCs values, while total WBCs and eosinophils were significantly increased in the diseased group than in healthy one. Biochemical analysis showed a significant decrease in serum antioxidant enzymes (CAT, SOD and GSH-Px) and a significant increase in serum DC and MDA in the diseased group as compared with the control group. Hormonal analysis showed a significant increase in ACTH, TSH, Cortisol and a significant decrease in serum T4 and T3. After treatment with Praziver, Sedemin and CoCl2, there was a significant effectiveness to maintain blood parameters within normal levels in the experimental group and increase reproductive outcome from these sheep

The blood parameters of steers of Kazakh White-headed breed against the background of the use of feed additive

2021 ◽  
pp. 16-23
Author(s):  
A. A. Volokhovich ◽  
R. R. Fatkullin
Keyword(s):  
Live Weight ◽  
Blood Parameters ◽  
Feed Additives ◽  
The Body ◽  
Feed Additive ◽  
Control Group ◽  
The Difference ◽  
High Level ◽  
Experimental Group ◽  
Percentage Ratio

Currently it has become popular to use different feed additives that allow us to increase the indicators of live weight gain by accelerating the metabolic processes in the body of animals, which allows us to take everything from the feed and to the maximum. With the advent of this trend in the market the range of feed additives in different directions has increased. For the research we have selected Vermiculite Expanded as a feed additive. The purpose of the research was to search the dynamics of changes in blood parameters in experimental steers in comparison with the control group against the background of the use of the feed additive Vermiculite Expanded. An assessment of blood parameters of Kazakh White-headed steers against the background of the use of mineral feed additive has been presented in the article. Three groups of steers have been formed for the research: the 1st group has been as a control and received the main economic ration; the 2nd and 3rd experimental groups received the feed additive to the main feeding ration in doses of 10 and 15 g/head for the groups, respectively. On the laboratory base blood tests we were able to search the correlation between morphological and biochemical parameters of blood and the growth rate of experimental steers. The steers of the 2nd and 3rd groups during the experiment had an advantage in blood serum indicators in terms of both total lipids and glucose, in the end, the difference in percentage ratio with the control group was 14,08 % in the 2nd group and 12,60 % in the 3rd experimental group. It is worth noting that the concentration of phosphorus and calcium in the blood was at the high level, which in our opinion reflects the influence of the feed additive Vermiculite Expanded.

scholarly journals Effects of Repeated Administration of Extracts from Arachis hypogaea Hulls on Blood Parameters and Histological Organization of Heart, Liver and Kidneys of Rats

2020 ◽  
pp. 20-33
Author(s):  
Djyh Bernard Nazaire ◽  
Tra Bi Irié Otis ◽  
Oungbé Monkoué Désiré ◽  
Gnahoué Goueh Laurent ◽  
Kra Adou Koffi Mathieu ◽  
...  
Keyword(s):  
Arachis Hypogaea ◽  
Biochemical Analysis ◽  
Histological Study ◽  
Experimental Period ◽  
Repeated Administration ◽  
Blood Parameters ◽  
Control Group ◽  
Aqueous Extracts ◽  
Subacute Toxicity

Aims: Arachis hypogaea (peanuts) is widely used in food worldwide. Therapeutic use of various parts of this plant has been mentioned in many traditional medicinal systems. The aim of the present study was to evaluate subacute toxicity of methanolic and aqueous extracts of peanuts hulls. Place and Duration: Laboratory of Biochemical Pharmacodynamics, Faculty of Biosciences, University Félix Houphouët-Boigny, from March to June 2018. Methodology: Serial extraction was done using methanol and water as solvents. The study was evaluated by orally daily doses of extracts 300, 600 and 1200 mg/kg. The treatment for 28 days concerned seven groups of animals, the control group and six treated groups. Each group included six animals, 3 males and 3 females. Animals of control group didn’t have any treatment. Animals were observed for general behavioural and signs of abnormalities during the experiment. After all treatments, blood was collected for haematological and biochemical analysis. Liver, kidney, and heart were removed, weighed for histological study. Results: The results showed that, there were not any significant (p˃0.05) changes in both the absolute and relative organ weights between the control and the test groups. Biochemical parameters were statistically equal in all groups. In addition, both extracts did not induce any significant effect on RBC and indices relating to it (HGB, PCV, MCV, MCH and MCHC) throughout the experimental period. But, there was a decrease (16.33±1.68) on WBC with methanolic extract compared to control (13.79±2.73). Histological examination of the liver, kidneys, and the heart showed normal organisation and structure of heart, kidneys and liver. Conclusion: It appears that the methanolic and aqueous extracts of hull of Arachis hypogaea did not produce any toxicity in oral subacute toxicity study. However, further studies are needed to confirm long term toxicities.

The Effects of a Caffeine Placebo and Experimenter Expectation on Blood Pressure, Heart Rate, Well-Being, and Cognitive Performance

2001 ◽  
Vol 6 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Harald Walach ◽  
Stefan Schmidt ◽  
Yvonne-Michelle Bihr ◽  
Susanne Wiesch
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Cognitive Task ◽  
Well Being ◽  
Control Group ◽  
Double Blind ◽  
Main Effect ◽  
The Difference ◽  
Being There ◽  
To Receive

We studied the effect of experimenter expectations and different instructions in a balanced placebo design. 157 subjects were randomized into a 2 × 4 factorial design. Two experimenters were led to expect placebos either to produce physiological effects or not (pro- vs. antiplacebo). All subjects except a control group received a caffeine placebo. They were either made to expect coffee, no coffee, or were in a double-blind condition. Dependent measures were blood pressure, heart rate, well-being, and a cognitive task. There was one main effect on the instruction factor (p = 0.03) with the group “told no caffeine” reporting significantly better well-being. There was one main effect on the experimenter factor with subjects instructed by experimenter “proplacebo” having higher systolic blood pressure (p = 0.008). There was one interaction with subjects instructed by experimenter “proplacebo” to receive coffee doing worse in the cognitive task than the rest. Subjects instructed by experimenter “antiplacebo” were significantly less likely to believe the experimental instruction, and that mostly if they had been instructed to receive coffee. Contrary to the literature we could not show an effect of instruction, but there was an effect of experimenters. It is likely, however, that these experimenter effects were not due to experimental manipulations, but to the difference in personalities.

Peritoneal Fluid and Plasma Fibrinolytic Activity in Women with Pelvic Inflammatory Disease

1992 ◽  
Vol 68 (02) ◽  
pp. 102-105 ◽  
Author(s):  
P J Dörr ◽  
E J P Brommer ◽  
G Dooijewaard ◽  
H M Vemer
Keyword(s):  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
Peritoneal Fluid ◽  
Fibrinolytic Activity ◽  
Degradation Products ◽  
Adhesion Formation ◽  
High Rate ◽  
Control Group ◽  
The Difference ◽  
Fibrinolytic Parameters

SummaryPrevious studies have shown that the fibrinolytic activity of peritoneum is depressed in local inflammation. We measured fibrinolytic parameters in peritoneal fluid and in plasma of 10 women with pelvic inflammatory disease (PID). Nine women, in whom laparoscopy for sterilisation was performed, served as a control group.In the peritoneal fluid of women with PID, PAI-Ag, t-PA-Ag and u-PA-Ag were many times higher than in the control group. In contrast to the antigens which may be present in inert complexes, the potentially active compounds, measured as t-PA activity and plasmin-activable scu-PA, were not significantly different in the two groups, and in none of the samples was the active enzyme tcu-PA detectable. Nevertheless, the mean peritoneal fluid TDP and FbDP concentrations were about twenty times higher in the PID group than in the control group. In plasma of PID patients, none of the parameters except u-PA-Ag differed from those in the control group. The difference between control and patient plasma u-PA-Ag was statistically significant, but too small to attach any relevance to the observation.Our data suggest that, in contrast to the classical concept of decreased fibrinolytic activity as a cause of adhesion formation, intraperitoneal fibrinolysis is enhanced in peritoneal inflammation through stimulation of the local production of t-PA and u-PA. Despite concomitant production of PAI, fibrinolysis occurs at a high rate, resulting in high levels of fibrin degradation products. Since this activated fibrinolysis does not meet the demand, therapeutic enhancement should be considered to prevent adhesions.

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