Subchronic Treatment of Combination Extract Detam 1 Soybean and Jati Belanda Leaves has No Toxic Effect on Function, Weight, and Histopathological of Wistar Rat Kidney

2016 ◽  
Vol 1 (4) ◽  
Author(s):  
Meilinah Hidayat ◽  
Sijani Prahastuti ◽  
Vibiola Chikita ◽  
Dewi Safitri ◽  
Siti F. Rahmawati ◽  
...  
Keyword(s):  
Kidney Function ◽  
Rat Kidney ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Good Effect ◽  
Control Group ◽  
Test Results ◽  
Treatment Groups ◽  
Subchronic Treatment ◽  
Significant Difference

The combination of Detam 1 soybean and Jati Belanda leaves proved to have effect of weight loss. Since it will become longterm using, toxicity effectvresearch is needed.  This study was conducted to determine the effect of subchronic combination of ethanol extract of soybean Detam 1 (EEDS) and Jati belanda (EEJB) on kidney function, with parameters: urea and creatinine, macroscopic and histopathological of kidney. This study were conducted on 120 Wistar rats (60 males, 60 females), 90 days (treatment group) and 120 days (satellite group). Rats were divided into six treatment groups. Test results of urea and creatinine levels of all treatment groups was lower than control group (p <0.05). The treatment turned out to have a good effect on kidney function. Kidney weight of all groups showed no significant difference with the control group (p> 0.05). Histopathological score interpretation’s result of low dose group male and female kidney rats were not disturbed. In conclusion, the combination of EEDS and EEJB  have a good effect on kidney function, did  not lead to changes in organ weight and at low doses did not cause disturbance of  renal histopathology. Key words: combination of soybean jati Belanda, subchronic treatment, kidney function, kidney’s  weight, kidney’s histopathology

scholarly journals SUBCHRONIC TOXICITY TEST OF COMBINATION ETHANOL EXTRACT OF DETAM 1 SOYBEAN (GLYCINE MAX L. MERR) AND JATI BELANDA (GUAZUMA ULMIFOLIA LAMK) TOWARD FUNCTION, WEIGHT, AND HISTOPATHOLOGICAL OF WISTAR RAT LIVER

2016 ◽  
Vol 9 (5) ◽  
pp. 197
Author(s):  
Meilinah Hidayat ◽  
Sijani Prahastuti ◽  
Estherolita Dewi ◽  
Dewi Safitri ◽  
Siti Farah Rahmawati ◽  
...  
Keyword(s):  
Liver Function ◽  
Toxicity Test ◽  
Low Dose ◽  
Ethanol Extract ◽  
Good Effect ◽  
Control Group ◽  
High Dose ◽  
Subchronic Toxicity ◽  
Treatment Groups ◽  
Significant Difference

ABSTRACTObjective: As an antiobesity therapy, combination extracts of Detam 1 soybean and Jati Belanda will be consumed for a long time; therefore, theirtoxicities to the liver need to be investigated. To determine the effect of subchronic toxicity test of combination of ethanol extract of Detam 1 soybean(EEDS) and ethanol extract of Jati Belanda (EEJB) on liver function with parameters: Alanine transaminase (ALT), macroscopic, and histopathologicalof liver.Methods: This study was conducted on 120 Wistar rats (60 males and 60 females), 90 days (treatment group) and 120 days (satellite group). Ratswere divided into six treatment groups (3 test materials, 1 control, and 2 satellites); each group included 10 males and 10 females.Results: ALT levels of treatment groups (low dose, medium, and high), both males and females were lower than the control group (p<0.05). Thetreatment groups demonstrated a good effects effect on liver function. Liver weight of all groups showed no significant difference compared with thecontrol group (p>0.05). Results of histopathological score interpretation of male and female liver rats of low dose groups were not disturbed; middledose groups were slightly disturbed and high dose groups were damaged. Satellite high doses of male groups were disrupted, while female groupswere not.Conclusion: The combination of EEDS and EEJB has a good effect on liver function, did not lead to change organ weight and at low doses did not causerenal histopathology damage in rats after 90 days administration.Keywords: Combination of soybean Jati Belanda, Toxicity subchronic test, Function, Weight, Histopathology, Liver.

scholarly journals Efek Nefroprotektif Ekstrak Etanol Bawang Putih (Allium sativum L.) pada Tikus yang Diinduksi Gentamisin

2020 ◽  
pp. 1-9
Author(s):  
Tanti Azizah Sujono ◽  
Fanny Atika Rizki
Keyword(s):  
Kidney Function ◽  
Allium Sativum ◽  
Ethanol Extract ◽  
Normal Control Group ◽  
Control Group ◽  
Oxygen Species ◽  
Concurrent Treatment ◽  
Treatment Groups ◽  
Modelling Method ◽  
Negative Controls

Gentamicin can induce damage to the kidney because of the accumulation of epithelial cells in the cortex. This accumulation can cause an increase in Reactive Oxygen Species (ROS), which in turn can trigger an increase in free radicals which causes a decrease in kidney function, which can be seen with Blood Urea Nitrogen (BUN) and sCr (serum creatinine) parameters. Garlic (Allium sativum L.) contains several bioactive compounds, including alliin, allisin, ajoene, and allil cysteine. The content of allisin has activity as an antioxidant. This study used a nephrotoxic mouse modelling method with 100 mg/kgBW of gentamicin induction intraperitoneally, then BUN and sCr levels were checked 24 hours after the 10th day induction. This study aimed to determine the effect of garlic ethanol extract in preventing the decline of renal function in rats induced by gentamicin with a parameter of BUN and sCr. The animal test were divided into 5 treatment groups, normal control group, negative controls, and three treatment groups at a dose of 50, 120, and 300 mg/kgBW for 10 days of concurrent treatment between extracts with gentamicin. The results of the study showed that garlic ethanol extract with a dose of  50, 120, 300 mg/kgBW was able to prevent a decrease  in kidney function due to the administration of gentamicin significantly when compared with negative controls (p 0,05).

scholarly journals Effect of Ethanol Extract of Mangosteen Peel on Serum Malondialdehyde and Leukotriene B4 Levels in Male White Rats (Rattus norvegicus) Wistar Strain Exposed with Electric Cigarette Smoke

2021 ◽  
Vol 56 (4) ◽  
pp. 283
Author(s):  
Jemima Lewi Santoso ◽  
Harianto Notopuro ◽  
Ema Qurnianingsih
Keyword(s):  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Leukotriene B4 ◽  
The Body ◽  
Control Group ◽  
Test Results ◽  
White Rats ◽  
Mangosteen Peel ◽  
Significant Difference

This study aims to analyze the administration of mangosteen peel ethanol extract at different doses can reduce levels of malondialdehyde (MDA) and leukotriene B4 (LTB4) serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke. Electric cigarette induces mitochondrial ROS production and triggers oxidative stress. Antioxidants are needed by the body if there are many free radicals, for example mangosteen peel. This laboratory experimental study uses a randomized post test only control group design. Thirty male white rats were divided into 5 groups randomly (KK, KR, P1, P2, P3). KK given 0.5% Na-CMC, KR given smoke and 0.5% Na-CMC, P1, P2, P3 given smoke and mangosteen peel ethanol extract 100, 200, 300 mg / kg weight. Data were tested for normality and homogeneity tests. Data distribution was found not normal. Data were tested by Kruskal-Wallis Test and Mann-Whitney. Kruskal-Wallis Test results showed significant differences in MDA and LTB4 levels in each experimental group (KS, KR, P1, P2 and P3), p <0.05. Mann-Whitney test results showed a significant difference in the MDA levels of the KR against P3 and in the LTB4 level of the KR against P2 (p <0.05). The conclusion of the study was the administration of mangosteen peel ethanol extract can reduce MDA and LTB4 levels serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke.

Antibacterial Effects of Calotropisgigantea (L) Extracts on Porphyromonasgingivalis

2020 ◽  
Vol 48 ◽  
pp. 111-118
Author(s):  
Zulfan M. Alibasyah ◽  
D.S. Ningsih ◽  
Sunnati ◽  
R. Andayani ◽  
N.P. Ranggaswuni
Keyword(s):  
Ethanol Extract ◽  
Dilution Method ◽  
Test Results ◽  
Treatment Groups ◽  
Significant Difference ◽  
The One ◽  
Dominant Bacteria ◽  
Post Hoc ◽  
Potential Test ◽  
Antibacterial Potential

The Porphyromonasgingivalis is one of the dominant bacteria that causes chronic periodontitis. These bacteria can penetrate the gingiva and cause tissue damage directly or indirectly with the induction of inflammation. The growth of Porphyromonasgingivalis can be inhibited by administrating the active compounds from plants that have an antibacterial effect such as Calotropisgigantea leaves.This study aimed to determine the antibacterial potential of Bidurileaves (Calotropisgigantea) extract against the Porphyromonasgingivalis ATCC. In this study the Biduri leaves were extracted by maceration method using 70% ethanol solvent. The antibacterial potential test of 70% ethanol extract of Biduri leaves (with a concentration of 5%, 10%, 15%, 20%, 25%, 30%, and 35%) against thePorphyromonasgingivalis was carried out using dilution method with 4 times repetition on Tryptone Soy Agar (TSA) media.The results of the study were analyzed using the One Way ANOVA test showing there were significant differences in the number of Porphyromonasgingivalis ATCC 33277 colonies due to administration of 70% ethanol extract of Bidurileaves (Calotropisgigantea) with p values <0,05. The test results were continued with the Post Hoc Tamhane test to determine the significant difference of the treatment groups. Based on the results of the study it could be concluded that 70% ethanol extract of Biduri leaves (Calotropisgigantea) had antibacterial potential against Porphyromonasgingivalis ATCC 33277 starting from the smallest concentration of 5% to the highest concentration of 35%.

The Effect of Moringa Oleifera Extract on Sperm Quality and Quantity of Diabetes Wistar Rat

2021 ◽  
Vol 2 (1) ◽  
pp. 91-96
Author(s):  
Novita Dwi Febrianty ◽  
Herisa Putri Anggraini ◽  
Meldawati ◽  
Boyke Marthin Simbolon
Keyword(s):  
Moringa Oleifera ◽  
Sperm Quality ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Control Group ◽  
P Value ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Chronic Disorder ◽  
Moringa Oleifera Leaves

A B S T R A C TDiabetes mellitus is a condition in which blood glucose levels increase in aperson's body which can result in chronic disorder. The effect of diabetes on amale reproductive system can affect sperm quality and disrupt the spermatogenesisprocess. Moringa oleifera leaves are rich in antioxidants such as flavonoids, vitaminA, vitamin E, and vitamin C, and contain selenium which helps lower bloodssugarlevels. This study was conductedtto determine the effect of administering Moringaoleifera leaf extract on the quality andiquantity ofssperms in Wistar rats with diabetesmellitus. This research is an experimental studyiusing pre-andipost-randomized,controlled group design. This study examinediblood sugarllevels, density,motility, andiviability ofssperm cells. This experimental test was dividediinto 4groups: one as a control group andtthree groups as treatment groups inducedwith alloxan and given ethanol extract of Moringa leaves with doses of 250, 500,and,750mg/kgBB. The results showed that the ethanol extract of Moringa leavesshowedssignificant improvements in various doses (P-value ≤ 0.05).

scholarly journals Peningkatan Pengetahuan Ibu-Ibu Rumah Tangga Dalam Perilaku Pengobatan Sendiri Untuk Penatalaksanaan Demam Dengan Metode Cara Belajar Ibu Aktif

2018 ◽  
Vol 1 (1) ◽  
pp. 27-30
Author(s):  
Rezqi Handayani
Keyword(s):  
Treatment Group ◽  
Mean Value ◽  
Pharmacological Therapy ◽  
Control Group ◽  
Test Results ◽  
Self Medication ◽  
Treatment Groups ◽  
Significant Difference ◽  
Level Of Knowledge ◽  
The Mean

Pharmacological therapy in fever patients is to provide Antipyretic drugs, can be done by using a doctor's prescription or by self-treatment. The condition that occurs in the community is self-medication conducted in the category of irrational treatment therapy because it is not in accordance with the condition of the patient. So, it is necessary to do education using Active Mother�s Ways of Learning Method to the housewives about rational fever self-treatment. The sample in this study were 40 housewives in the Flamboyant Bawah, divided into two major groups namely the control and treatment groups. Each group was given a pretest and posttest in a questionnaire of fever management. In the treatment group, fever drug education given before posttest by used CBIA method. The results showed an increase in knowledge of housewives from before education and after education was seen from the acquisition of grades. The mean pretest value in the control group was 74.38 and the treatment group was 69.69. Statistical test results show there was no significant difference in the mean value between the control group and the treatment group. The mean posttest value in the control group was 77.81 and the treatment group was 87.50. There was a significant difference between the mean value of the control group and treatment group. From the results of the statistical analysis showed that the level of knowledge of the treatment group given education by used the CBIA method produced a higher value than the control group that was not given education.

scholarly journals Establishment of a male Wistar rat model of nanobacteriainduced kidney stones

2021 ◽  
Vol 18 (5) ◽  
pp. 1061-1068
Author(s):  
Biao Qian ◽  
Gaurab Pokhrel ◽  
Qinzhang Wang ◽  
Jihong Liu
Keyword(s):  
Rat Model ◽  
Kidney Stones ◽  
Kidney Stone ◽  
Stone Formation ◽  
Rat Kidney ◽  
Weight Ratio ◽  
Wistar Rat ◽  
Control Group ◽  
Significant Difference ◽  
Caudal Vein

Purpose: To establish a male Wistar rat model of nanobacteria (NB)-induced kidney stones. Methods: Sixty male Wistar rats were randomly divided into control group (NC group) given caudal vein injection of saline + saline gavage, and NB-induced stone group (NBS group) given caudal vein injection of NB + saline gavage. Results: Compared with NC, serum creatinine, blood uric acid, urea nitrogen and urinary calcium levels in NBS group increased between weeks 3 and 8 (p < 0.05). Kidney index (kidney weight/body weight ratio) in the NBS group was higher than that in NC group from weeks 8-10. At week 8, urine pH and serum phosphorus in NBS group were higher than those in NC group (p < 0.05). Between weeks 6 and 7, serum calcium in NBS group was higher than that in NC group (p < 0.05). Calcium crystals in NBS rats were distributed mostly in the distal and proximal convoluted tubules. However, no such crystals were observed in NC rats. Similarly, no such pathological changes were seen in the renal tissue of NC group. Calculus analysis showed that stone formation was higher in NBS group than in NC group (p < 0.05). There was no significant difference in micro-CT between the two groups (p ˃ 0.05). Conclusion: The successful establishment of the Wistar rat kidney stone model using NB cultured from urine of upper urinary tract stone patient is potentially useful for further etiological studies on kidney stone formation.

scholarly journals UJI ANTI INFLAMASI SECARA TOPIKAL EKSTRAK ETANOL UMBI SARANG SEMUT (Myrmecodia tuberosa Jack) PADA MENCIT PUTIH JANTAN

2017 ◽  
Vol 5 (1) ◽  
pp. 103-112
Author(s):  
Muhammad Islamoyo ◽  
Yufri Aldi ◽  
Surya Nelis
Keyword(s):  
Ethanol Extract ◽  
Negative Control ◽  
Fluid Volume ◽  
Tissue Response ◽  
Male Rats ◽  
Control Group ◽  
Treatment Groups ◽  
Edema Fluid ◽  
Significant Difference ◽  
Anti Inflammation

Inflammation  is a tissue response  towards  damaging  physical  or chemical  stimulations.  One local sign of inflammation  is  edema  (tumor).  Usage  of  natural  herbal  medicine  has  become  an  alternative  way  to  prevent inflammation.  The extract of umbi sarang semut (Myrmecodia  tuberosa Jack) has anti-inflammation,  anti-bacterial and  antioxidant  properties.  This  study  is  to  determine  the  anti-inflammation  effect  of  the  topical  application  of Myrmecodia  tuberosa Jack ethanol extract. This study used 28 white male rats previously  induced with carragenin 2%  b/v  0,5ml  and  divided  into  4  treatment  groups.  Groups  1,2  dan  3  were  given  the  ethanol  extract  with  a concentration  of 0,5 %; 1%; 2% respectively  after induction and on 24, 48 and 72 hours after the first application. Group 4 was the untreated control group. Each treatment group were extracted for their inflammation  exudate fluid from their backs using a 1 ml syringe. Edema fluid volume was measured. The results showed a decrease of edema fluid volume on all treatment groups.   Statistical data analysis showed a significant difference between group 2 and the negative  control;  also between  group  3 and the negative  control.  In conclusion,  the topical  ethanol  extract  of Myrmecodia tuberosa Jack can reduce inflammation reactions. Keywords: Myrmecodia  tuberosa  Jack, anti-inflammation,  topical, edema fluid volume

scholarly journals Hippocampal Brain Derived Neurothropic Factor Levels in Response to Anaerobic Physical Exercises

2017 ◽  
Vol 6 (2) ◽  
pp. 134
Author(s):  
Mohammad Zulkarnain ◽  
Rostika Flora ◽  
Juliastuti Juliastuti ◽  
Anita Apriany ◽  
Dewi Pujiana ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Cognitive Functions ◽  
Wistar Rat ◽  
Treadmill Running ◽  
Control Group ◽  
Bdnf Level ◽  
Treatment Groups ◽  
Physical Exercises ◽  
Significant Difference ◽  
Post Test

Anaerobic physical exercise does not only improve body fitness, but it also increases cognitive functions. The increase of cognitive functions is mediated by the increase of Brain Derived Neurothropic Factor (BDNF) level. Several studies revealed that anaerobic physical exercise can increase the BDNF level inhippocampal tissues. However, it is still questionable whether or notthe frequency of exercise influences the production of BDNF inhippocampal tissues. This study was to find out the BDNF mean level inhippocampal tissue of Wistarrats which were treated with anaerobic physical exercises conducted in different frequencies.This study used post test only control groupdesign. The research subject was 28 rats of Rattusnorvegicus strain wistar divided into 4 groups; 1 control group and 3 treatment groups (one-time, three-time, and five times a week treatment). The anaerobic physical exercise used animal treadmill running in the speed of 35m/min for 20 minutes at four-minute intervals. The mean levels of BDNF were increased in all treatment groups compared with control group (151.588 vs 57.059). The highest mean level of hippocampal tissue BDNF was found in the treatment group of three times a week (177.701). There was a significant difference in BDNF mean levels of Wistar rat brain tissues among the treatment groups.

scholarly journals Hippocampal Brain Derived Neurothropic Factor Levels in Response to Anaerobic Physical Exercises

2017 ◽  
Vol 6 (2) ◽  
pp. 136
Author(s):  
Mohammad Zulkarnain ◽  
Rostika Flora ◽  
Juliastuti Juliastuti ◽  
Anita Apriany ◽  
Dewi Pujiana ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Cognitive Functions ◽  
Wistar Rat ◽  
Control Group ◽  
Bdnf Level ◽  
Treatment Groups ◽  
Physical Exercises ◽  
Significant Difference ◽  
Post Test ◽  
The Mean

Anaerobic physical exercise does not only improve body fitness, but it also increases cognitive functions. The increase of cognitive functions is mediated by the increase of <em>Brain Derived Neurothropic Factor</em> (BDNF) level. Several studies revealed that anaerobic physical exercise can increase the BDNF level inhippocampal tissues. However, it is still questionable whether or notthe frequency of exercise influences the production of BDNF inhippocampal tissues. This study was to find out the BDNF mean level inhippocampal tissue of Wistarrats which were treated with anaerobic physical exercisesconducted in different frequencies.This study used <em>post test only control groupdesign. </em>The research subject was 28 rats of<em>Rattusnorvegicus strain wistar</em>divided into 4 groups; 1 control group and 3 treatment groups (one-time, three-time, and five times a week treatment). The anaerobic physical exercise used <em>animal treadmill</em>running in the speed of 35m/min for 20 minutes at four-minute intervals. The mean levels of BDNF were increased in all treatment groups compared with control group (151.588 vs 57.059). The highest mean level of hippocampal tissue BDNF was found in the treatment group of three times a week (177.701). There was a significant difference in BDNF mean levels of Wistar rat brain tissues among the treatment groups.<p> </p>

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