IN VITRO TEST OF MANNOSE SPECIFIC ADHESIN (MSA) of Lactobacillus sp. AND ETHANOL EXTRACT OF SKIN FRUIT Garcinia mangostana L. BY USING Saccharomyces cerevisiae

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
T. Yulinery., dkk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Human Life ◽  
Ethanol Extract ◽  
In Vitro Test ◽  
Garcinia Mangostana ◽  
Fruit Skin ◽  
Infection Disease ◽  
Vitro Test

Infection disease is a threat for human life. Research on preventing infection disease is very important. The aim this research was to improve the preventing of infection disease. This research was the basic on identification of MSA activity. The in vitro result of MSA which was isolated from mangosteen (Garcinia mangostana L.) and the extract of the fruit skin byusing Saccharomyces cerevisiae. The result showed that there were 11 isolates of potential Lactobacillus, that have variation in MSA activities. Four isolates of them showed that the higher activities between 9.6-11.8 were TM1, BST1, BST2, and BST5. The MSA activities showed by reducing coagulation after adding D-Mannose. The highest activities showedby isolate TM1, and the lowest showed by isolate BST7. By using this method it is clearly found that the skin fruit not contain MSAkeywords : mannose-specific adhesin (msa ), probiotic, Saccharomyces cerevisiae.

scholarly journals UJI AKTIFITAS ANTIBAKTERI EKSTRAK ETANOL KULIT BUAH MANGGIS (Gracinia mangostana L.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli

2018 ◽  
pp. 7
Author(s):  
Putri Permata ◽  
Retno Kawuri ◽  
AA Ketut Darmadi
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Human Colon ◽  
In Vitro Test ◽  
Clear Zone ◽  
Garcinia Mangostana ◽  
Body Tissues ◽  
Vitro Test ◽  
Inhibitory Power

Mangosteen rind has a benefical compounds liked xanthone which consisting of mangostin, mangosterol, mangostinon A and B, trapezifolixanthone, tovophyllin B, alpha and beta mangostin, garcinon B, mangostanol, gartanin, and flavonoid epikatekin that known contain an antibacterial and antidiare agent. Escherichia coliis an opportunistic bacterium that is commonly found in the human colon as a normal flora. Its unique because it can cause primary infection in the intestine such as diarrhea in children, as well as its ability to cause infection in other body tissues outside the intestine.This research was done from December 2016 to March 2017. The methods that used in this research were well diffusion test to know the inhibitory power by measuring the clear zone formed. measure the clear zone of mangosteen rind against Escherichia coli. Then to testing extract compounds, there was used phytochemicals screening test. Data there has been found will be analyzed through analysis of variance (ANOVA). In the inhibitory test of ethanol extract from mangosteen rind (G. mangostana L.), it was so effective to inhibit the growth of Escherichia coli through in-vitro test with a resistivity of 1,58 cm and categorized as a strong inhibitory, which MIC from mangosteen rind against Escherichia coli was 3,9% and the compounds that contain in ethanol extract from mangosteen rind (G. mangostana L.) were alkaloid, phenolik, flavonoid, saponin and terpenoid. Key word : antibacterial activity, Garcinia mangostana L., Escherichia coli.

scholarly journals Stabilitas Fisik Sediaan Emulgel Ekstrak Etanol Daging Buah Limpasu (Baccaurea lanceolata (Miq.) Müll. Arg.)

2019 ◽  
Vol 6 (2) ◽  
pp. 8
Author(s):  
Prima Happy Ratnapuri ◽  
Fajrina Haitami ◽  
Mia Fitriana
Keyword(s):  
High Temperature ◽  
Statistical Analysis ◽  
Room Temperature ◽  
Physical Stability ◽  
Ethanol Extract ◽  
In Vitro Test ◽  
Study Results ◽  
Vitro Test ◽  
Sunscreen Product

ABSTRAK Ekstrak etanol daging buah limpasu (Baccaurea lanceolata (Miq.). Müll. Arg.) telah teruji memiliki aktivitas tabir surya secara in vitro, sehingga diformulasikan dalam bentuk sediaan emulgel dengan variasi konsentrasi (% b/b) ekstrak etanol daging B.lanceolata FI (4%), FII (5%) dan FIII (6%). Sediaan emulgel yang telah dibuat selanjutnya perlu dilakukan uji stabilitas fisik saat penyimpanan. Penelitian ini bertujuan untuk menentukan stabilitas fisik sediaan emulgel ekstrak etanol daging buah B. lanceolata selama penyimpanan. Uji stabilitas fisik dilakukan selama 28 hari pada suhu tinggi 40°±2°C dan suhu ruang 28°C±2°C dengan evaluasi meliputi uji organoleptis, uji pH, uji daya sebar, uji daya lekat dan uji viskositas pada hari ke-0, 7, 14, 21 dan 28. Analisis secara statistik dilakukan dengan software SPSS 21 pada taraf kepercayaan 95%. Hasil penelitian pada formula I, II, dan III dengan variasi konsentrasi ekstrak menunjukkan bahwa penyimpanan selama 28 hari pada suhu tinggi 40°±2°C dan ruang 28°C±2°C tidak mempengaruhi kestabilan pH, viskositas, daya sebar dan daya lekat gel (p>0,050). Kesimpulan dari penelitian ini menunjukkan bahwa sediaan emulgel ekstrak etanol daging B. lanceolata stabil secara fisik selama 28 hari pada suhu tinggi 40°±2°C dan suhu ruang 28°C±2°C. Kata Kunci: Baccaurea lanceolata, ekstrak etanol, emulgel, stabilitas fisik.  ABSTRACT Limpasu (Baccaurea lanceolata (Miq.). Müll. Arg.) fructus ethanol extract has been reported as sunscreen activity by in vitro test, so that it could be formulated in sunscreen product with their concentration variances FI (4%), FII (5%) and FIII (6%) (% b/b). The further this preparation needs to be tested for physical stability during storage. This study aimed to determine the emulgel stability physically of B.lanceolata fructus ethanol extract during storage. Physical stability test was performed at high temperature 40°±2°C and room temperature of 28°±2oC during 28 days with evaluation including organoleptic, pH, dispersive, adhesion power and viscosity test on days 0, 7, 14, 21 and 28. Statistical analysis, SPSS 21 software at 95% confidence level. This study results, formula I, II, and III with their concentration variances showed that storage for 28 days at high temperature 40°±2°C and 28°±2° C didn’t affect the pH, viscosity, dispersive, adhesion power stability (p>0,050). The conclusion of this study showed that the emulgel preparation of B. lanceolata fructus ethanol extract were physically stable for 28 days at high temperature of 40°±2°C and room temperature of 28°C±2°C. Keywords: Baccaurea lanceolata, ethanol extract, emulgel, physical stability

scholarly journals In Vitro Test of Antibacterial Ethanol Extract, n-Hexane Fraction and Ethyl acetate Fraction of Sungkai Leaf (Peronema cenescens) Against Salmonella typhi

1970 ◽  
Vol 8 (3) ◽  
pp. 59-61
Author(s):  
Sitepu Nadroh Br.
Keyword(s):  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Salmonella Typhi ◽  
Hexane Fraction ◽  
In Vitro Test ◽  
Leaf Powder ◽  
Vitro Test

Object: This study aims to look at the class of compounds and the comparison of the antibacterial activity of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Methods: Study included phytochemical screening and in vitro antibacterial testing of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Results: obtained groups of chemical compounds alkaloids, flavonoids, glycosides, anthraquinones, tannins and triterpenoids/steroids on Sungkai leaf powder. Ethanol extract of Sungkai leaves obtained resistance at a concentration of 20% by 12.7 mm, and inhibition of the ethyl acetate fraction at a concentration of 20% of 14.8 mm. Conclusion: Ethyl acetate fraction of Sungkai leaves have antibacterial properties against S. typhi which is greater than ethanol extract and hexane fraction of leaf heal.  

scholarly journals In-vitro and In-vivo Determinations of Sun Protection Factors (SPF) of Skin Lotions Containing Mountain Papaya Fruit and Mangosteen Peel Ethanolic Extract

2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Heru Sasongko ◽  
Natasya Advaita ◽  
Ratih Guswinda Lestari ◽  
Karimah Umar Aidid
Keyword(s):  
Sun Exposure ◽  
Sun Protection ◽  
In Vitro Test ◽  
Garcinia Mangostana ◽  
In Vivo Test ◽  
Antioxidants Activity ◽  
Mangosteen Peel ◽  
Vitro Test

Indonesia is a high sun exposure country. Exposure to ultraviolet (UV) causes various kinds of skin disorders such as erythema, sunburn, aging, and cancer. Mountain papaya fruit (Vasconcellea pubescens A.DC.) and mangosteen peel (Garcinia mangostana L.) contains metabolite compounds that can protect the skin from sunlight because of its antioxidants activity. The purpose of this study to determine whether the combination of the mountain papaya fruit and mangosteen peel extracts in skin lotion can be used as sun protectors through the in-vitro and in-vivo study. The experiment was done by extracting the mountain papaya fruit and mangosteen peel through the maceration method. The extracts were formulated into skin lotion in three different formulas with the ratio of mountain papaya fruit extract: mangosteen peel extract as follows F1(1:1), F2(1:3), and F3(3:1). In vitro test was done by using UV-VIS spectrophotometry to determine the SPF value and in vivo test was used erythema-induced rats by exotera beam light. The result of in vitro test gained a high enough SPF value for all three formulas F1=23,23; F2=21,70 and F3=28,64 and the result of in vivo test showed that all three formulas did not indicate the existence of erythema value.         It can be concluded that three skin lotion formulas containing mountain papaya fruit and mangosteen peel ethanol extract have the effect of sun protection.

scholarly journals ANALISIS FITOKIMIA DAN UJI POTENSI EKSTRAK BUAH RENGGAK (Amomum Dealbatum) SEBAGAI PESTISIDA NABATI TERHADAP JAMUR Pyricularia Oryzae DAN BAKTERI Xanthomonas oryzae

2020 ◽  
Vol 8 (1) ◽  
pp. 115
Author(s):  
Novita Hidayatun Nufus
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Positive Control ◽  
Pyricularia Oryzae ◽  
Xanthomonas Oryzae ◽  
In Vitro Test ◽  
Paper Disk ◽  
Vitro Test

This study aims to determine the potency of Renggak (Amomum dealbatum) extract as bio pesticide against Pyricularia oryzae and Xanthomonas oryzae. Therefore an in vitro of  antifungal and antibacterial activity of the Renggak ethanol extract were carried out against Pyricularia oeyzae and Xanthomonas oryzae. In vitro antifungal  analysis were done using agar diffusing method by dissolving Renggak extract (1%, 5%, and 10%) into fungal medium and the growth of  Pyricularia oryzae were observed. In vitro antibacterial analysis were complited using agar diffusing method with paper disk that contain Renggak extract (10%, 20%, and 30%) and antibiotic chloroamfenikol as positive control. The result suggest that Renggak fruit extract has antifungal and antibacterial activities. The treatment with 10% Renggak extract could inhibit the growth of Pyricularia oryzae to 100%, same with positive control (Fungicide Score-250). In vitro test for antibacterial activity showed that treatments with 20% and 30% Renggak extract gave inhibitory activities that similar with positive control. Renggak ethanol extract gave positive results on Flavonoid, Alkaloid, Steroid, Terpenoid, and Saponin tests. GC-MS assays showed that Renggak ethanol extract produces at least 10 organic compounds that was identified as  2-butanone, 4-methoxypheyl, Benzenepropanoic-aci, 4-hydroxyphenil, CAS, octadecanoid acid, stearic acid, palmiti acid, Benzenepropanoic acid, dan Farnesol isomer A, which have antimicrobial avtivities.

scholarly journals FORMULATION OF SOLAR CREAM EXTRACT ETHANOL GANGGANG GREEN (Ulva lactuca L.) AND IN VITRO TEST OF SPF VALUE (SUN PROTECTING FACTOR)

2021 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Dinda Ayu Pratiwi ◽  
Emelda Emelda Emelda ◽  
Saddam Husein Husein
Keyword(s):  
Green Algae ◽  
Ethanol Extract ◽  
Ulva Lactuca ◽  
In Vitro Test ◽  
Extract Concentration ◽  
Vitro Test ◽  
Maximum Protection ◽  
Harmful Effects ◽  
Uv Rays

<p>Excessive ultraviolet radiation or UV rays will make the epidermal tissue in the skin unable to resist the harmful effects. Sunscreen is a cosmetic ingredient that functions as a physical or chemical function as a barrier to the penetration of UV rays into the skin. Plants that can function as sunscreens because they have phenolic compounds, one of which is green algae (<em>Ulva lactuca</em> L).</p><p>This type of research is an experimental study using UV-Vis spectrophotometry to determine the ability of sun cream to inhibit UV radiation to the skin. The purpose of this study was to formulate sunscreen cream from ethanol extract of green algae (<em>Ulva lactuca</em> L.) and measure the best SPF (<em>Sun Protecting Factor</em>) value of various concentrations of green algae (Ulva lactuca L.) ethanol extract sunscreen cream.</p><p>The results of the study the SPF value of formula 1 (cream base) was 1.5077, which means formula 1 has no activity as sunscreen, formula 2 (3% green algae extract) with formula 2 (3% extract extract) with an SPF value of 7,311 which is classified as in the extra protection category, formula 3 (4% extract concentration) has an SPF value of 13,427 which is included in the maximum protection category and formula 4 with an SPF value of 19,282 which belongs to the ultra protection category. The cream formulation with the highest SPF value was formula 4. The higher the extract concentration, the higher the SPF value.</p>

Haemostatic Platelet Plug Formation in the Isolated Rabbit Mesenteric Preparation - An Analysis of Red Blood Cell Participation

1980 ◽  
Vol 44 (01) ◽  
pp. 006-008 ◽  
Author(s):  
D Bergqvist ◽  
K-E Arfors
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Adenosine Diphosphate ◽  
In Vitro Test ◽  
Pharmacological Agents ◽  
Vitro Test ◽  
Releasing Effect ◽  
Plug Formation

SummaryIn a model using an isolated rabbit mesenteric preparation microvessels were transected and the time until haemostatic plugs formed was registered. Perfusion of platelet rich plasma gave no haemostasis whereas whole blood did. Addition of chlorpromazine or adenosine to the whole blood significantly prolonged the time for haemostasis, and addition of ADP to the platelet rich plasma significantly shortened it. It is concluded that red cells are necessary for a normal haemostasis in this model, probably by a combination of a haemodynamic and ADP releasing effect.The fundamental role of platelets in haemostatic plug formation is unquestionable but there are still problems concerning the stimulus for this process to start. Three platelet aggregating substances have been discussed – thrombin, adenosine diphosphate (ADP) and collagen. Evidence speaking in favour of thrombin is, however, very minimal, and the discussion has to be focused on collagen and ADP. In an in vitro system using polyethylene tubings we have shown that "haemostasis" can be obtained without the presence of collagen but against these results can be argued that it is only another in vitro test for platelet aggregation (1).To be able to induce haemostasis in this model, however, the presence of red blood cells is necessary. To further study this problem we have developed a model where haemostatic plug formation can be studied in the isolated rabbit mesentery and we have briefly reported on this (2).Thus, it is possible to perfuse the vessels with whole blood as well as with platelet rich plasma (PRP) and different pharmacological agents of importance.

EKSTRAK KULIT BUAH MANGGIS (Garcinia Mangostana L.) SEBAGAI OBAT ANTI DIARE

2019 ◽  
Vol 2 (1) ◽  
pp. 9-13
Author(s):  
Zaim Anshari ◽  
Chrismis Novalinda Ginting ◽  
Linda Chiuman ◽  
Yuliani Mardiati Lubis
Keyword(s):  
Blood Vessels ◽  
Smooth Muscles ◽  
Latin Square ◽  
Ethanol Extract ◽  
Garcinia Mangostana ◽  
Pharmacology And Toxicology ◽  
The Difference ◽  
North Sumatra ◽  
Papaverine Hydrochloride

This study aims to determine whether mangosteen rind extract (in the form of ethanol extract/EE) can be used as an anti-diarrhea drug after compared with other anti-diarrhea substances in three experimental groups. This research is an in vitro experimental study using adult male guinea pigs weighing 400-600 gr through the standard method of Magnus with the Latin square controlled experiment design. The study was conducted at the Pharmacology and Toxicology Laboratory of the Faculty of Pharmacy, University of North Sumatra. The results showed that the contraction of ileum in Ach with Atp + Ach compared the difference in contraction of ileum Ach with EE + Ach showed the difference in difference between the two contractions of the ileum was significant, the contraction of ileum in His with Dip + His compared indifference in contraction of ileum His with EE + His showed a difference indifference. the two ileal contractions are significant, the ileal contraction in the bar with Papa + Bar compared to the difference between the ileum bar contraction with EE + Bar shows no difference in the difference between the two ileum contractions. The conclusion is that the Mangosteen Skin Ethanol Extract works similarly to Papaverine Hydrochloride which is an antidiarrheal drug used to relax smooth muscles so that it can also make blood vessels dilate by relaxing smooth muscles in the walls of blood vessels.

scholarly journals In Vitro Newly Isolated Environmental Phage Activity against Biofilms Preformed by Pseudomonas aeruginosa from Patients with Cystic Fibrosis

2021 ◽  
Vol 9 (3) ◽  
pp. 478
Author(s):  
Ersilia Vita Fiscarelli ◽  
Martina Rossitto ◽  
Paola Rosati ◽  
Nour Essa ◽  
Valentina Crocetta ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Multidrug Resistant ◽  
In Vitro Test ◽  
Chronic Infections ◽  
Hospital Environments ◽  
Vitro Test ◽  
General Reliability ◽  
Phage Activity

As disease worsens in patients with cystic fibrosis (CF), Pseudomonas aeruginosa (PA) colonizes the lungs, causing pulmonary failure and mortality. Progressively, PA forms typical biofilms, and antibiotic treatments determine multidrug-resistant (MDR) PA strains. To advance new therapies against MDR PA, research has reappraised bacteriophages (phages), viruses naturally infecting bacteria. Because few in vitro studies have tested phages on CF PA biofilms, general reliability remains unclear. This study aimed to test in vitro newly isolated environmental phage activity against PA isolates from patients with CF at Bambino Gesù Children’s Hospital (OBG), Rome, Italy. After testing in vitro phage activities, we combined phages with amikacin, meropenem, and tobramycin against CF PA pre-formed biofilms. We also investigated new emerging morphotypes and bacterial regrowth. We obtained 22 newly isolated phages from various environments, including OBG. In about 94% of 32 CF PA isolates tested, these phages showed in vitro PA lysis. Despite poor efficacy against chronic CF PA, five selected-lytic-phages (Φ4_ZP1, Φ9_ZP2, Φ14_OBG, Φ17_OBG, and Φ19_OBG) showed wide host activity. The Φ4_ZP1-meropenem and Φ14_OBG-tobramycin combinations significantly reduced CF PA biofilms (p < 0.001). To advance potential combined phage-antibiotic therapy, we envisage further in vitro test combinations with newly isolated phages, including those from hospital environments, against CF PA biofilms from early and chronic infections.

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