UJI AKTIFITAS ANTIBAKTERI EKSTRAK ETANOL KULIT BUAH MANGGIS (Gracinia mangostana L.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli

Mangosteen rind has a benefical compounds liked xanthone which consisting of mangostin, mangosterol, mangostinon A and B, trapezifolixanthone, tovophyllin B, alpha and beta mangostin, garcinon B, mangostanol, gartanin, and flavonoid epikatekin that known contain an antibacterial and antidiare agent. Escherichia coliis an opportunistic bacterium that is commonly found in the human colon as a normal flora. Its unique because it can cause primary infection in the intestine such as diarrhea in children, as well as its ability to cause infection in other body tissues outside the intestine.This research was done from December 2016 to March 2017. The methods that used in this research were well diffusion test to know the inhibitory power by measuring the clear zone formed. measure the clear zone of mangosteen rind against Escherichia coli. Then to testing extract compounds, there was used phytochemicals screening test. Data there has been found will be analyzed through analysis of variance (ANOVA). In the inhibitory test of ethanol extract from mangosteen rind (G. mangostana L.), it was so effective to inhibit the growth of Escherichia coli through in-vitro test with a resistivity of 1,58 cm and categorized as a strong inhibitory, which MIC from mangosteen rind against Escherichia coli was 3,9% and the compounds that contain in ethanol extract from mangosteen rind (G. mangostana L.) were alkaloid, phenolik, flavonoid, saponin and terpenoid. Key word : antibacterial activity, Garcinia mangostana L., Escherichia coli.

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IN VITRO TEST OF MANNOSE SPECIFIC ADHESIN (MSA) of Lactobacillus sp. AND ETHANOL EXTRACT OF SKIN FRUIT Garcinia mangostana L. BY USING Saccharomyces cerevisiae

Jurnal Rekayasa Lingkungan ◽

10.29122/jrl.v8i1.1978 ◽

2018 ◽

Vol 8 (1) ◽

Author(s):

T. Yulinery., dkk

Keyword(s):

Saccharomyces Cerevisiae ◽

Human Life ◽

Ethanol Extract ◽

In Vitro Test ◽

Garcinia Mangostana ◽

Fruit Skin ◽

Infection Disease ◽

Vitro Test

Infection disease is a threat for human life. Research on preventing infection disease is very important. The aim this research was to improve the preventing of infection disease. This research was the basic on identification of MSA activity. The in vitro result of MSA which was isolated from mangosteen (Garcinia mangostana L.) and the extract of the fruit skin byusing Saccharomyces cerevisiae. The result showed that there were 11 isolates of potential Lactobacillus, that have variation in MSA activities. Four isolates of them showed that the higher activities between 9.6-11.8 were TM1, BST1, BST2, and BST5. The MSA activities showed by reducing coagulation after adding D-Mannose. The highest activities showedby isolate TM1, and the lowest showed by isolate BST7. By using this method it is clearly found that the skin fruit not contain MSAkeywords : mannose-specific adhesin (msa ), probiotic, Saccharomyces cerevisiae.

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In vitro efficacy of several disinfectants against Salmonella enterica serovar Enteritidis and Escherichia coli strains from poultry

Ciência Rural ◽

10.1590/0103-8478cr20151288 ◽

2016 ◽

Vol 46 (8) ◽

pp. 1438-1442

Author(s):

Sonia Martínez-Martínez ◽

Sheila Yubero-Delgado ◽

Elías-Fernando Rodríguez-Ferri ◽

Rafael Frandoloso ◽

Álvaro Álvarez-Estrada ◽

...

Keyword(s):

Escherichia Coli ◽

Salmonella Enterica ◽

Environmental Control ◽

Control Measures ◽

In Vitro Test ◽

Salmonella Enterica Serovar Enteritidis ◽

Vitro Test ◽

The Individual ◽

Ammonium Compounds

ABSTRACT The efficacy of 28 individual or blended disinfectants against avian Salmonella enterica serovar Enteritidis and Escherichia coli strains was determined. An in vitro test in the presence and absence of serum as source of organic material was conducted. Povidone-iodine (releasing 1% available iodine), 1% potassium permanganate, 70% ethanol, 2% chlorhexidine digluconate and three commercial formulations based on quaternary ammonium compounds + formaldehyde or cresol derivates were the most effective against all strains tested and reduced bacterial counts by more than 106 times (6-log10) regardless of the presence of organic matter. These commercial compounds as well as ethanol and chlorhexidine among the individual substances tested might be helpful in the adoption of environmental control measures against these two enterobacteria in poultry industry.

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Stabilitas Fisik Sediaan Emulgel Ekstrak Etanol Daging Buah Limpasu (Baccaurea lanceolata (Miq.) Müll. Arg.)

Jurnal Pharmascience ◽

10.20527/jps.v6i2.7345 ◽

2019 ◽

Vol 6 (2) ◽

pp. 8

Author(s):

Prima Happy Ratnapuri ◽

Fajrina Haitami ◽

Mia Fitriana

Keyword(s):

High Temperature ◽

Statistical Analysis ◽

Room Temperature ◽

Physical Stability ◽

Ethanol Extract ◽

In Vitro Test ◽

Study Results ◽

Vitro Test ◽

Sunscreen Product

ABSTRAK Ekstrak etanol daging buah limpasu (Baccaurea lanceolata (Miq.). Müll. Arg.) telah teruji memiliki aktivitas tabir surya secara in vitro, sehingga diformulasikan dalam bentuk sediaan emulgel dengan variasi konsentrasi (% b/b) ekstrak etanol daging B.lanceolata FI (4%), FII (5%) dan FIII (6%). Sediaan emulgel yang telah dibuat selanjutnya perlu dilakukan uji stabilitas fisik saat penyimpanan. Penelitian ini bertujuan untuk menentukan stabilitas fisik sediaan emulgel ekstrak etanol daging buah B. lanceolata selama penyimpanan. Uji stabilitas fisik dilakukan selama 28 hari pada suhu tinggi 40°±2°C dan suhu ruang 28°C±2°C dengan evaluasi meliputi uji organoleptis, uji pH, uji daya sebar, uji daya lekat dan uji viskositas pada hari ke-0, 7, 14, 21 dan 28. Analisis secara statistik dilakukan dengan software SPSS 21 pada taraf kepercayaan 95%. Hasil penelitian pada formula I, II, dan III dengan variasi konsentrasi ekstrak menunjukkan bahwa penyimpanan selama 28 hari pada suhu tinggi 40°±2°C dan ruang 28°C±2°C tidak mempengaruhi kestabilan pH, viskositas, daya sebar dan daya lekat gel (p>0,050). Kesimpulan dari penelitian ini menunjukkan bahwa sediaan emulgel ekstrak etanol daging B. lanceolata stabil secara fisik selama 28 hari pada suhu tinggi 40°±2°C dan suhu ruang 28°C±2°C. Kata Kunci: Baccaurea lanceolata, ekstrak etanol, emulgel, stabilitas fisik. ABSTRACT Limpasu (Baccaurea lanceolata (Miq.). Müll. Arg.) fructus ethanol extract has been reported as sunscreen activity by in vitro test, so that it could be formulated in sunscreen product with their concentration variances FI (4%), FII (5%) and FIII (6%) (% b/b). The further this preparation needs to be tested for physical stability during storage. This study aimed to determine the emulgel stability physically of B.lanceolata fructus ethanol extract during storage. Physical stability test was performed at high temperature 40°±2°C and room temperature of 28°±2oC during 28 days with evaluation including organoleptic, pH, dispersive, adhesion power and viscosity test on days 0, 7, 14, 21 and 28. Statistical analysis, SPSS 21 software at 95% confidence level. This study results, formula I, II, and III with their concentration variances showed that storage for 28 days at high temperature 40°±2°C and 28°±2° C didn’t affect the pH, viscosity, dispersive, adhesion power stability (p>0,050). The conclusion of this study showed that the emulgel preparation of B. lanceolata fructus ethanol extract were physically stable for 28 days at high temperature of 40°±2°C and room temperature of 28°C±2°C. Keywords: Baccaurea lanceolata, ethanol extract, emulgel, physical stability

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CHITOSAN INHIBITION TEST AGAINST E. coli AND DIGESTIBILITY OF THE RATION IN THE IN-VITRO METHOD

Jurnal Pendidikan Matematika dan IPA ◽

10.26418/jpmipa.v11i2.37996 ◽

2020 ◽

Vol 11 (2) ◽

pp. 230

Author(s):

Eli Sahara ◽

Sofia Sandi ◽

Fitra Yosi

Keyword(s):

Escherichia Coli ◽

Crude Protein ◽

Dry Matter ◽

Control Treatment ◽

Clear Zone ◽

Bacterial Colony ◽

E Coli ◽

Chicken Feces ◽

Inhibitory Power

Diarrhea and vomiting are often caused by E coli bacteria. E coli bacteria has a strain of Shigatoxigenic Escherichia coli (STEC), producing Shiga poisons or poisons such as Shiga (verotoxin) which are harmful and pollute nature. This strain of the E coli bacterium has a detrimental effect because it excludes one or both types of Shiga Like Toxin -1 (Stx -1) and Shiga Like Toxin-2 (Stx-2) toxins. This bacterial infection has the potential as a zoonotic agent because it has been found in feces and sheep meat, feces and beef meat, chicken feces and human feces. If this bacterial colony inceases in the digestive tract of poultry it will disturb the productivity of the livestock. Therefore it must be watched out and studied more deeply. The objectives of the study are 1) to see the inhibitory power of chitosan on the growth and development of E coli bacteria in vitro 2) the test of digestibility of dry matter (BK) and crude protein (PK) ration in vitro. The treatments given in this test are: R0 = control (without chitosan), R1 = 0.5% chitosan, R2 = 1% chitosan, R3 = 1.5% chitosan, R4 = 2% chitosan, R5 = 2.5% chitosan. The parameters measured were 1) inhibition of chitosan against E. coli growth based on clear zone diameter 2) digestibility of dry matter (BK) and crude protein (PK) ration in vitro. The results showed that the higher level of chitosan administration showed greater inhibition, which was indicated by the greater diameter of the clear zone caused. The provision of 2.5% chitosan shows medium inhibition that is has a range of 10-14 mm. The addition of a dose of 1.5% chitosan in the ration was able to increase the digestibility of dry matter by 7.86% and the digestibility of crude protein 11.20% higher than the control treatment (without chitosan). The conclusion of this study is that chitosan can inhibit the growth of E coli and improve the digestibility of dry matter (BK) and crude protein (PK) for the better.AbstrakPenyakit diare dan muntah-muntah sering disebabkan oleh bakteri E coli. Bakteri E coli memiliki strain Shigatoxigenic Escherichia coli (STEC), menghasilkan racun Shiga atau racun seperti Shiga (verotoxin) yang berbahaya dan mencemari alam. Strain dari bakteri E coli ini mempunyai efek merugikan karena mengeluarkan salah satu atau kedua jenis toxin Shiga Like Toxin -1 (Stx -1) maupun Shiga Like Toxin-2 (Stx-2). Infeksi bakteri ini berpotensi sebagai agen zoonosis karena sudah pernah ditemukan pada feses dan daging domba, feses dan daging sapi serta feses ayam dan feses manusia. Jika koloni bakteri ini tinggi dalam saluran pencernaan unggas akan mengganggu produktivitas ternak tersebut. Oleh sebab itu harus diwaspadai dan dikaji lebih mendalam. Tujuan penelitian adalah 1) melihat daya hambat kitosan terhadap pertumbuhan dan perkembangan bakteri E coli secara in vitro 2) menguji kecernaan bahan kering (BK) dan protein kasar (PK) ransum secara in vitro. Perlakuan yang diberikan dalam pengujian ini adalah: R0 = kontrol (tanpa kitosan), R1 = 0,5% kitosan, R2 = 1 % kitosan, R3 = 1,5% kitosan, R4 = 2% kitosan, R5 = 2,5% kitosan. Parameter yang diukur adalah 1) daya hambat kitosan terhadap pertumbuhan E. coli berdasarkan diameter zona bening (in vitro) 2) kecernaan bahan kering (BK) dan protein kasar (PK) ransum secara in vitro. Hasil penelitian menunjukkan bahwa semakin tinggi level pemberian kitosan menunjukkan daya hambat yang semakin besar yang ditandai oleh semakin besarnya diameter zona bening yang ditimbulkan. Pemberian 2,5% kitosan menunjukkan daya hambat sedang yaitu memiliki range 10 - 14 mm. Penambahan dosis 1,5% kitosan dalam ransum, mampu meningkatkan kecernaan bahan kering 7,86% dan kecernaan protein kasar 11,20% lebih tinggi dari perlakuan kontrol (tanpa kitosan). Kesimpulan penelitian ini adalah bahwa kitosan mampu menghambat pertumbuhan E coli dan meningkatkan kecernaan bahan kering (BK) serta protein kasar (PK) menjadi lebih baik.Kata kunci: Kitosan, daya hambat, E. coli, kecernaan ransum, in vitro

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EFEKTIVITAS EKSTRAK BUAH SAWO, BAWANG PUTIH, DAUN ANDONG, DAN BUAH PARE TERHADAP PERTUMBUHAN BAKTERI ESCHERICHIA COLI

JURNAL MEDIA KESEHATAN ◽

10.33088/jmk.v7i2.238 ◽

2018 ◽

Vol 7 (2) ◽

pp. 144-149

Author(s):

Susiwati Susiwati

Keyword(s):

Escherichia Coli ◽

Inhibition Zone ◽

Garlic Extract ◽

In Vitro Test ◽

E Coli ◽

Significant Difference ◽

Vitro Test ◽

Inhibition Zones ◽

Escherichia Coli Bacteria

This research aims to determine the inhibition of sapodilla fruit, garlic, andong leaves and pare fruit toward the growth of escherichia coli bacteria. Antimicroba test used paper disc diffusion was in-vitro test. Sapodilla fruit, garlic, andong leaves and pare fruit were extracted by using maceration process. The extracts were tested on the growth of E- coli bacteria. The highest inhibition zone (6,7mm) was found in andong leaves extract. The highest inhibition zone was 8.3 mm, whereas the inhibition of pare fruit did not not provide the inhibitory zone. It can be concluded that garlic extract, sapodolla extract and decoction of andong leaves have highly inhibitory in vitro. Based on stastistical analysis, there was Significant difference betwen the effectiveness of garlic extract with a decoction of andong leaves but the effectivess of garlic extract with sapodilla extract was not meaningful. Whereas pare fruit did not give any inhibition zones. From the result of this research, the society can be encouraged to consume andong leaves or sapodilla fruit to treat diarrhea. In addition, garlic spices and pare fruit also can be used to overcome diarrhea, which is caused by the bacterium E. coli.

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In Vitro Test of Antibacterial Ethanol Extract, n-Hexane Fraction and Ethyl acetate Fraction of Sungkai Leaf (Peronema cenescens) Against Salmonella typhi

Asian Journal of Pharmaceutical Research and Development ◽

10.22270/ajprd.v8i3.757 ◽

1970 ◽

Vol 8 (3) ◽

pp. 59-61

Author(s):

Sitepu Nadroh Br.

Keyword(s):

Ethyl Acetate ◽

Antibacterial Properties ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Salmonella Typhi ◽

Hexane Fraction ◽

In Vitro Test ◽

Leaf Powder ◽

Vitro Test

Object: This study aims to look at the class of compounds and the comparison of the antibacterial activity of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Methods: Study included phytochemical screening and in vitro antibacterial testing of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Results: obtained groups of chemical compounds alkaloids, flavonoids, glycosides, anthraquinones, tannins and triterpenoids/steroids on Sungkai leaf powder. Ethanol extract of Sungkai leaves obtained resistance at a concentration of 20% by 12.7 mm, and inhibition of the ethyl acetate fraction at a concentration of 20% of 14.8 mm. Conclusion: Ethyl acetate fraction of Sungkai leaves have antibacterial properties against S. typhi which is greater than ethanol extract and hexane fraction of leaf heal.

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In-vitro and In-vivo Determinations of Sun Protection Factors (SPF) of Skin Lotions Containing Mountain Papaya Fruit and Mangosteen Peel Ethanolic Extract

Majalah Obat Tradisional ◽

10.22146/mot.49931 ◽

2020 ◽

Vol 25 (3) ◽

Author(s):

Heru Sasongko ◽

Natasya Advaita ◽

Ratih Guswinda Lestari ◽

Karimah Umar Aidid

Keyword(s):

Sun Exposure ◽

Sun Protection ◽

In Vitro Test ◽

Garcinia Mangostana ◽

In Vivo Test ◽

Antioxidants Activity ◽

Mangosteen Peel ◽

Vitro Test

Indonesia is a high sun exposure country. Exposure to ultraviolet (UV) causes various kinds of skin disorders such as erythema, sunburn, aging, and cancer. Mountain papaya fruit (Vasconcellea pubescens A.DC.) and mangosteen peel (Garcinia mangostana L.) contains metabolite compounds that can protect the skin from sunlight because of its antioxidants activity. The purpose of this study to determine whether the combination of the mountain papaya fruit and mangosteen peel extracts in skin lotion can be used as sun protectors through the in-vitro and in-vivo study. The experiment was done by extracting the mountain papaya fruit and mangosteen peel through the maceration method. The extracts were formulated into skin lotion in three different formulas with the ratio of mountain papaya fruit extract: mangosteen peel extract as follows F1(1:1), F2(1:3), and F3(3:1). In vitro test was done by using UV-VIS spectrophotometry to determine the SPF value and in vivo test was used erythema-induced rats by exotera beam light. The result of in vitro test gained a high enough SPF value for all three formulas F1=23,23; F2=21,70 and F3=28,64 and the result of in vivo test showed that all three formulas did not indicate the existence of erythema value. It can be concluded that three skin lotion formulas containing mountain papaya fruit and mangosteen peel ethanol extract have the effect of sun protection.

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ANALISIS FITOKIMIA DAN UJI POTENSI EKSTRAK BUAH RENGGAK (Amomum Dealbatum) SEBAGAI PESTISIDA NABATI TERHADAP JAMUR Pyricularia Oryzae DAN BAKTERI Xanthomonas oryzae

Bioscientist : Jurnal Ilmiah Biologi ◽

10.33394/bjib.v8i1.2661 ◽

2020 ◽

Vol 8 (1) ◽

pp. 115

Author(s):

Novita Hidayatun Nufus

Keyword(s):

Antibacterial Activity ◽

Ethanol Extract ◽

Antibacterial Activities ◽

Positive Control ◽

Pyricularia Oryzae ◽

Xanthomonas Oryzae ◽

In Vitro Test ◽

Paper Disk ◽

Vitro Test

This study aims to determine the potency of Renggak (Amomum dealbatum) extract as bio pesticide against Pyricularia oryzae and Xanthomonas oryzae. Therefore an in vitro of antifungal and antibacterial activity of the Renggak ethanol extract were carried out against Pyricularia oeyzae and Xanthomonas oryzae. In vitro antifungal analysis were done using agar diffusing method by dissolving Renggak extract (1%, 5%, and 10%) into fungal medium and the growth of Pyricularia oryzae were observed. In vitro antibacterial analysis were complited using agar diffusing method with paper disk that contain Renggak extract (10%, 20%, and 30%) and antibiotic chloroamfenikol as positive control. The result suggest that Renggak fruit extract has antifungal and antibacterial activities. The treatment with 10% Renggak extract could inhibit the growth of Pyricularia oryzae to 100%, same with positive control (Fungicide Score-250). In vitro test for antibacterial activity showed that treatments with 20% and 30% Renggak extract gave inhibitory activities that similar with positive control. Renggak ethanol extract gave positive results on Flavonoid, Alkaloid, Steroid, Terpenoid, and Saponin tests. GC-MS assays showed that Renggak ethanol extract produces at least 10 organic compounds that was identified as 2-butanone, 4-methoxypheyl, Benzenepropanoic-aci, 4-hydroxyphenil, CAS, octadecanoid acid, stearic acid, palmiti acid, Benzenepropanoic acid, dan Farnesol isomer A, which have antimicrobial avtivities.

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