scholarly journals The Potentials of Limau (Citrus amblycarpa Hassk. Ochse) as A Functional Food and Ornamental Mini Tree Based on Metabolomic and Morphological Approaches

2017 ◽  
Vol 4 (2) ◽  
pp. 49-57
Author(s):  
Rahmat Budiarto ◽  
Roedhy Poerwanto ◽  
Edi Santosa ◽  
Darda Efendi
Keyword(s):  
Functional Food ◽  
Octadecenoic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Citrus Species ◽  
Leaf Extracts ◽  
Aesthetic Value ◽  
Fruit Extract ◽  
Aromatic Content ◽  
One Year ◽  
Fragrance Compounds

Limau (Citrus amblycarpa (Hassk.) Ochse) is one of the native Citrus species of West Java that is grown in the local backyard, thus is easily found in local cuisines. Limau has great potentials for use as a potted plant in home gardens for its aesthetic value and fragrance. The objective of this research was to describe the metabolites fingerprint and morphology of Limau as a potential functional and ornamental mini tree. Methanol leaf extracts from one-year-old Limau originated from Bogor were analyzed using untargeted Gas Chromatography Mass Spectrometry (GCMS). Methanol leaf extract was dominated by vitamin E and composed of other 18 metabolites including phytosterols, fatty acids and terpenes. The major portion of fruit extract consisted of 6-octadecenoic acid, palmitate and various fragrance compounds such as alpha sinensal, alpha limonene, beta citronellal, citronellol, and sabinene. Limau fruits are also a functional food due to its rich antioxidant and aromatic content in the fruits. The morphology of limau tree is suitable as a potted ornamentals or mini-fruits tree for its small crown, sparse branching with unifoliate aromatic leaves and small spines, and plentiful aromatics mini fruits with the diameter of around 2-4 cm.  

scholarly journals Analysis of Bioactive Chemical Compounds of Leaves Extracts from Tamarindus indica Using FT-IR and GC-MS Spectroscopy

2021 ◽  
pp. 22-34
Author(s):  
Mansour Abdulnabi H. Mehdi ◽  
Abdul-hakim M. A. Al- Alawi ◽  
Ahmed Zain A. Thabet ◽  
Fadel Y. S. Alarabi ◽  
Gozif Mohammed N. Omar ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Vaccenic Acid ◽  
Octadecenoic Acid ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Tamarindus Indica ◽  
Octadecanoic Acid ◽  
Leaf Extracts ◽  
Phytochemical Compounds ◽  
Ft Ir

Tamarindus indica is one of the medicinal plants used in the treatment of various diseases traditionally. Aims: This study was conducted to identify the phytochemical constituents of T. indica leaf extracts. Methods: Using Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS) to identification of bioactive compounds in extracts of T. indica. Results: The FT-IR spectrum confirmed the presence of alcohol group, alkene group, amine group, carbonates, ethers, carboxylic acid and disulfides in both extracts. A total of 22 and 38 bioactive phytochemical compounds were identified in the ethanolic and aqueous extracts of T. indica, respectively. The major bioactive compounds of the ethanolic extract of T. indica leaves were cis-Vaccenic acid, trans-13-Octadecenoic acid, Oleic Acid, Octadecanoic acid, Octadecanoic acid, 2-(2-hydroxyethoxy) ethyl ester, Eicosanoic acid and Eicosane, 1-Iodo-2-methylundecane, 10-Methylnonadecane. While the major bioactive compounds of the aqueous extract were 3-O-Methyl-d-glucose, Myo-Inositol, 4C-methyl-, Myo-Inositol, 2-C-methyl-, Propane, 2,2-dimethoxy-, 1,3-Dioxolane, Ethanol, 2-(1-methylethoxy)-, and 2-Pentanone, 4-hydroxy-4-methyl-, 2-Hexanol, 2-methyl-, Ethanamine, N-methyl.

scholarly journals A review on the efficacy of Ocimum gratissimum, Mentha spicata, and Moringa oleifera leaf extracts in repelling mosquito

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Modupe Elizabeth Ojewumi ◽  
Oyinlola Rukayat Obanla ◽  
Daniel Mfon Atauba
Keyword(s):  
Moringa Oleifera ◽  
Octadecenoic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
List Type ◽  
Active Components ◽  
Leaf Extracts ◽  
Mentha Spicata ◽  
Ocimum Gratissimum ◽  
Synthetic Drugs ◽  
Toxicity Effects

Abstract @@@In recent times, repellents and synthetic drugs have been identified as having negative toxicity effects on humans and the environment. Apart from the unfavourable effects on man and livestock caused by these chemicals-based (synthetic) repellents, they are also expensive, non-biodegradable, and no more effective because mosquitoes are getting adapted. With these drawbacks, an eco-friendly plant-based insecticide as a substitute is needed urgently. This paper reviews the extraction and use of essential oil from the leaves of Mentha spicata, Ocimum gratissimum, and Moringa oleifera as mosquito repellent. Carvone, Eugenol, and 9-Octadecenoic acid were discovered to be the most active components in the M. spicata, O. gratissimum, and M. oleifera extracts, respectively, using gas chromatography-mass spectrometry (GC-MS). Highlights In recent times, repellents and synthetic drugs have been identified as having negative toxicity effects on humans and the environment. Apart from the unfavorable effects on man and livestock caused by these chemical-based (synthetic) repellents, they are also expensive, non-biodegradable, and no more effective because mosquitoes are getting adapted. An eco-friendly plant-based insecticide as a substitute is needed urgently. Diseases transmitted by mosquitoes are still a significant reason for the global mortality rate, with over 700 million individuals experiencing such diseases every year. With the proper formulation of other repellent forms using their oils, they can replace non-degradable synthetic mosquito repellents since they are eco-friendly. In general, the mosquitocidal activity and percentage protection of plant extract increase with increasing concentration of the extracts in different formulations. This paper is our original work. We certify that this manuscript has not been published in part or whole elsewhere in any language, and it has not been submitted to any other journal for reviews.

A new furan carboxamide and two potential precursors from a terrestrial streptomycete

2017 ◽  
Vol 72 (3) ◽  
pp. 175-182 ◽  
Author(s):  
Humaira Naureen ◽  
Michel Feussi Tala ◽  
Khaled A. Shaaban ◽  
Mohamed Shaaban ◽  
Anja Schüffler ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Density Functional ◽  
Acid Amide ◽  
Octadecenoic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Metabolites ◽  
Cytotoxic Activities ◽  
Tetradecanoic Acid ◽  
Vinyl Phenol ◽  
New Compounds

Abstract Three new bioactive metabolites, 1,6-dihydroxy- 2-methyl-heptan-4-one (1), 4-hydroxy-1-(2-methyl-oxiranyl)- pentan-2-one (2), and 2-(2-hydroxy-propyl)-4-methylfuran- 3-carboxylic acid amide (3) were isolated from the terrestrial Streptomyces sp. isolate ANK245, along with the new microbial constituent p-vinylanisol (4a) and the known metabolites p-vinyl-phenol (4b) and phenethyl alcohol. Analysis of the nonpolar part of the extract by gas chromatography/mass spectrometry (GC-MS) provided further evidence for tetradecanoic acid, 9-octadecenoic acid, hexadecanoic acid, 2-methoxy-4-vinylphenol (4c), 4-hydroxy-3-methoxy-benzaldehyde, o-hydroxybiphenyl, and 1,5,9-trimethyl-4,8,13-cyclotetradecatrien-1,3-diol (5). Structures 1–3 of the new compounds were elucidated by nuclear magnetic resonance (NMR) and NMR spectroscopy, but mass spectrometry (MS) techniques and their absolute configuration were determined by density functional theory (DFT) calculations and Mosher derivatisation. Their antimicrobial and cytotoxic activities were evaluated in comparison with the crude bacterial extract.

scholarly journals GC-MS ANALYSIS AND ANTIFUNGAL ACTIVITY OF METHANOLIC ROOT EXTRACT OF Chenopodium album AGAINST Sclerotium rolfsii

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
A. ALI ◽  
A. JAVAID ◽  
A. SHOAIB
Keyword(s):  
Antifungal Activity ◽  
Methyl Ester ◽  
Environmental Pollution ◽  
Chenopodium Album ◽  
Octadecenoic Acid ◽  
Sclerotium Rolfsii ◽  
Gas Chromatography Mass Spectrometry ◽  
Root Extract ◽  
Malt Extract ◽  
Ms Analysis

ABSTRACT Sclerotium rolfsii is a soil-borne fungal plant pathogen that causes diseases in more than 500 plant species. Chemical fungicides used to control this disease cause environmental pollution, therefore, plant derived compounds can be used as alternative to synthetic fungicides to reduce environmental pollution. Chenopodium album is a weed of family Chenopodiaceae that is used as food and also has medicinal importance. In the present study, antifungal activity of methanolic root extract of C. album was evaluated against S. rolfsii using six concentrations viz. 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 g 100 mL-1 amended in malt extract as growth medium. All the root extract concentrations significantly reduced fungal biomass by 15-58% over control. Gas chromatography-mass spectrometry (GC-MS) analysis of the methanolic root extract of C. album was performed. Six compounds were identified in methanolic root extract through GC-MS analysis. The most abundant compound was 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester (58.56%) followed by 9-octadecenoic acid (Z)-, methyl ester (12.75%) and 9-octadecenoic acid (Z)-, methyl ester (10.27%), which might be responsible for antifungal activity of methanolic root extract of C. album.

scholarly journals Sandalwood, an Indian medicinal plant attenuates the microbial growth and influence up/down regulation of the metabolites

2021 ◽  
Author(s):  
Romana Parveen ◽  
Tooba Naz Shamsi ◽  
Sadaf Fatima
Keyword(s):  
Methyl Ester ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Trimethylsilyl Ester ◽  
E Coli ◽  
Acid Methyl ◽  
Soxhlet Apparatus ◽  
Antibacterial Assay

AbstractThe methanolic extract of sandalwood (SwME) was prepared by soxhlet apparatus and the antibacterial assay was performed. Further, the metabolite profiling of SwME and lysates of E. coli and E. coli grown in the presence of SwME was generated. SwME showed maximum inhibition against E. coli (MTCC 443) i.e. 82.71%, and minimal against B. subtilis (MTCC 736) i.e. 26.82%. The metabolome profiles of E. coli and SwME were generated using gas chromatography-mass spectrometry (GC-MS) technique. Comparative studies were done to understand to what extent metabolite modifications differ between SwME, E. coli lysate and the E. coli strain grown in presence of extract. Result revealed 23 peaks with major compounds present in E. coli were 9-Octadecenoic Acid (Z)-, Methyl Ester (26.85%), Hexadecanoic Acid, methyl ester (20.5%) and Hexadecanoic acid, trimethylsilyl ester (15.79%). When E. coli was grown in the presence of SwME, GC-MS analysis showed 25 peaks with major compounds such as 9-Octadecenoic Acid, Methyl Ester (21.97%), Hexadecanoic Acid, Methyl Ester (17.03%), and Hexadecanoic Acid, Trimethylsilyl Ester (14.96%). Correlating the metabolic profiles with the changes occurring is essential to progression their comprehension and in the development of new approaches to identify the metabolomics regulation in E. coli in response to SwME.

scholarly journals Volatile Compounds and Antioxidant and Antimicrobial Activities of Selected Citrus Essential Oils Originated from Nepal

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6683
Author(s):  
Devi Prasad Bhandari ◽  
Darbin Kumar Poudel ◽  
Prabodh Satyal ◽  
Karan Khadayat ◽  
Sital Dhami ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Citrus Sinensis ◽  
Antioxidant Properties ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Citrus Species ◽  
Citrus Peel ◽  
Antioxidant And Antimicrobial Activities

Citrus species of plants are among the most commercially cultivated crops, mainly for their fruit. Besides, the generally consumed flesh inside the fruit, the peel is quite important too. Essential oils extracted from the peel have a history of being used by humankind for centuries. These essential oils are rich in antioxidants and antimicrobial agents. Comparative investigation of volatile constituents, and antioxidant and antimicrobial activities were undertaken. The essential oils were evaluated through gas chromatography-mass spectrometry (GC–MS), and enantiomeric composition by chiral GC–MS. Similarly, the antioxidant properties were evaluated by 2,2-diphenyl-1-picrylhydrazyl scavenging assay, and antimicrobial activities were assayed using the disk diffusion method. The highest extraction yield of 1.83% was observed in Citrus sinensis Osbeck. GC–MS analysis showed limonene (63.76–89.15%), γ-terpinene (0.24–6.43%), β-pinene (0.15–6.09%), linalool (0.35–3.5%), sabinene (0.77–2.17%), myrcene (0.74–1.75%), α-terpineol (0.28–1.15%), and α-pinene (0.2–0.58%) as the major constituents of the essential oil of the Citrus species studied. For the first time, through our study, chiral terpenoids have been observed from Citrus grandis Osbeck essential oil. The order of antioxidant activity is as follows: Citrus grandis Osbeck red flesh > Citrus reticulata Blanco > Citrus sinensis Osbeck > Citrus grandis Osbeck white flesh. Except for Citrus grandis Osbeck white flesh (52.34 µL/mL), all samples demonstrated stronger antioxidant activities than those of the positive control, quercetin (5.60 µL/mL). Therefore, these essential oils can be used as a safe natural antioxidant to prevent product oxidation. Likewise, citrus peel essential oil showed antimicrobial activity against tested bacterial strains, albeit marginal.

scholarly journals Biological activity of wild Ligularia fischeri leaf extracts in the development of functional food materials

2018 ◽  
Vol 25 (3) ◽  
pp. 359-365
Author(s):  
Su-Bin Lim ◽  
Myung-Uk Kim ◽  
Kyeung-Il Park ◽  
Eun-Ho Lee ◽  
Ye-Jin Kim ◽  
...  
Keyword(s):  
Biological Activity ◽  
Functional Food ◽  
Leaf Extracts ◽  
Ligularia Fischeri

scholarly journals Bioactivity and Volatile Profiling of Azadirachta indica Leaves for the Management of Maize Weevil, Sitophilus zeamais (Motsch.) Infestations

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
A.G.W.U. Perera ◽  
M.M.S.C. Karunaratne ◽  
S.D.M. Chinthaka
Keyword(s):  
Azadirachta Indica ◽  
Solid Phase ◽  
Biological Activities ◽  
Sitophilus Zeamais ◽  
Gas Chromatography Mass Spectrometry ◽  
Solid Phase Micro Extraction ◽  
Leaf Extracts ◽  
Maize Weevil ◽  
Biological Potential ◽  
Wide Range

Neem (Azadirachta indica A. juss), is known to possess a wide range of pharmacological properties and is thus commercially exploitable. Apart from its medicinal potential, a considerable progress has been achieved regarding biological potential and chemical composition of the leaves which is an ever-increasing interest to the scientific community. During this study, biological phenomena and secondary metabolite composition of A. indica leaves were examined in the management of Sitophilus zeamais on stored maize. Insecticidal and repellent potential of A. indica leaf powders were evaluated in both contact and fumigant forms. Phytochemical screening of 11 phyto constituents was performed following the standard procedures for n-hexane, dichloromethane, ethyl acetate, methanol and aqueous leaf extracts. Volatile profile of A. indica leaves was characterized by employing headspace-solid-phase micro extraction coupled with gas chromatography-mass spectrometry (HS-SPME/GC-MS). Over 60% weevil repellency was recorded at doses above 23.33%, whereas 100% and 67% contact and fumigation mortalities were observed respectively, 9 days after treatment at the dose of 33.33% and the respected LD50 values were 1.56 g and 4.48 g. Thirty two volatile compounds were identified in three distinct chemical classes (Monoterpenoid, sesquiterpenoid and purine nucleosides). γ-Elemene (24.06%), 3,7 (11)-eudesmadiene (6.83%), caryophyllene (6.40%), and 10s,11s-himachala-3(12),4-diene (6.36%) were the major constituents of neem leaf volatiles, followed by other compounds present in less than 4% which might be responsible for varied biological activities observed. Thus the odour impact of the bioassayguided study clearly implies that A. indica leaves can be harnessed against S. zeamais infestations.Keywords: Azadirachta indica, Sitophilus zeamais, headspace-solid-phase micro-extraction, insecticidal activity, repellency

scholarly journals The Application of Headspace Solid-phase Microextraction as a Preparation Approach for Gas Chromatography with Mass Spectrometry

2020 ◽  
Vol 69 (9-10) ◽  
pp. 515-520
Author(s):  
Igor Jerković ◽  
Marina Kranjac ◽  
Marina Zekić ◽  
Ani Radonić ◽  
Zvonimir Marijanović
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Markers ◽  
Headspace Solid Phase Microextraction ◽  
Natural Organic Compounds ◽  
The University ◽  
Fragrance Compounds

Reviewed in brief are the selected results of the application of headspace solid-phase microextraction as a preparative approach for gas chromatography – mass spectrometry (HS-SPME/GC-MS) for natural organic compounds research at the University of Split, Faculty of Chemistry and Technology. A wide variety of headspace compounds from different natural sources has been identified: lower aliphatic compounds (e.g., C5- and C6-compounds), aromatic compounds, monoterpenes (e.g., linalool derivatives (oxides, anhydro-oxides, epoxides), hotrienol), sesquiterpenes (e.g., eudesmol isomers, hydrocarbons), and C9- and C13-norisoprenoids (e.g., 3,4-dihydro-3-oxoedulan, 4-oxoisophorone, trans-β-damascenone). These compounds are important phytochemicals as flavour/fragrance compounds, chemical markers of the botanical origin or others (e.g., allelochemicals, pheromones, or acaricide residue).

scholarly journals Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria

2020 ◽  
Vol 16 (2) ◽  
Author(s):  
P. M. Ridzuan ◽  
Hairul Aini Hamzah ◽  
Anis Shah ◽  
Norazian Mohd Hassan ◽  
Baharudin Roesnita
Keyword(s):  
Pathogenic Bacteria ◽  
Synergistic Effects ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Synergistic Activity ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Bacteria Growth ◽  
Gram Positive Bacteria

Antibacterial activity of different types of P. odorata leaf extracts was evaluated in combination with standard antibiotics. Persicaria. odorata leaves were extracted with n-hexane (n-hex), dichloromethane (DCM) and methanol (MeOH).  Each extract was applied on vancomycin (30µg), erythromycin (15µg) and gentamicin (10µg) discs, respectively. Disk diffusion method was used to evaluate the synergistic activity of each combination on Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli. Minimum inhibitory concentration (MIC) and gas chromatography mass spectrometry (GCMS) analysis was performed on the active extract. Synergistic effects seen were mainly from the n-hex+antibiotics combinations, mainly on the Gram-positive bacteria (7 additive, 5 antagonistic), with MIC range from 50 µg/ml to 100 µg/ml, as well as Gram-negative bacteria (2 additive, 2 indifferent, 5 antagonistic). In particular, synergism showed by the combination of n-hex+van were all additive against the susceptible bacteria. DCM extract combination showed synergistic effects on three Gram-positive species (S. aureus, S. epidermidis, S. pyogenes). Meanwhile, MeOH+antibiotics combination showed significant additive synergistic effects (p<0.05) on S. aureus and S. epidermidis.  The major compounds of leaves extract were decanal and β-citral. n-Hex extract superiorly inhibited Gram-positive bacteria growth as compared to DCM and MeOH extracts. The additive synergistic property of the n-hex P. odorata extract could be further studied for possible use as an antibacterial agent.

Sign in / Sign up

Export Citation Format

Share Document