scholarly journals Evaluation of Inhibition Activity of Rosmerinus officinalis plant watery and oily extracts on some pathogenic Microorganisms

2009 ◽  
Vol 33 (2) ◽  
pp. 36-40
Author(s):  
Auroba M.S. Ibrahim
Keyword(s):  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Growth Inhibition ◽  
Aqueous Extract ◽  
Aeromonas Hydrophila ◽  
Salmonella Typhi ◽  
Pathogenic Microorganisms ◽  
Inhibition Activity ◽  
Active Ingredients ◽  
Chemical Test

This study included evaluation of inhibition activity of watery and oily extracts of Rosmerinusofficinalis plant on some pathogenic microorganisms like Salmonella typhi، Escheichia coli ، Staphylococcusaureus ، Klebsiella pneumoniae، Aeromonas hydrophila ، Candida albicans. The results of oily extract showedmore activity at concentration (40mg/ml) of microorganisms growth inhibition (26, 24,22,18,18)mmrespectively, compared with aqueous extract which recorded (12,12,10,9,8)mm respectively at concentration(200mg/ml). And procedure the qualitative chemical test of some active ingredients in Rosmerinus officinalisplant. The results showed that the plant contan glycosides, alkaloids, flavonoides , phenols, tannins , resins,saponins and comarins

scholarly journals Inhibition Activity of Garlic (Allium sativum) Skin Aqueous Extract on Mastitis Causing Microorganisms

2020 ◽  
Vol 21 (1) ◽  
pp. 38
Author(s):  
Afduha Nurus Syamsi ◽  
Meyta Pratiwi ◽  
Aras Prasetiyo Nugroho
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aqueous Extract ◽  
Design Research ◽  
Inhibition Activity ◽  
Animal Science ◽  
Minimum Concentration ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Streptococcus Mutants

Mastitis in dairy caused by microorganisms, e.g. Staphylococcus aureus and Candida albicans, is a serious problem due to its effect in decreasing quality and quantity of milk production up to 53.5%. Mastitis precautions are generally carried out using iodine solution and has risk leaving residue both on the udder and milk produced. Natural antimicrobial compounds need to be developed to solve iodine residues. An alternative natural ingredient that can be used is the skin of garlic which is known contains allicin, that is able to inhibit the growth of pathogenic microorganisms. This completely randomized design research by applying Kirby-Bauer method, aimed to identify the effect and concentration of garlic skin extracts that are effective against the growth of Staphylococcus aureus, Streptococcus mutants, Escherichia coli and Candida albicans. Research was done in 6 months in Faculty of Animal Science and Faculty of Biology Unsoed, and the datas were analyzed using ANOVA. Garlic skins were extracted using aquadest to three concentrations i.e. 5%, 10% and 15%, and were tested on to mastitis-causing microorganisms growth. The results showed the extract of garlic skins using aquadest has high potency in inhibiting the growth of mastitis-causing microorganisms. Based on the results, aqueous extract of garlic skin has inhibition activity with minimum concentration 5-10%.

scholarly journals Efficacy of Aqueous Extract of Pistia Stratiotes (Water Lettuce) Against some Tropical Fish Bacterial

2021 ◽  
Author(s):  
Adelakun Kehinde Moruff ◽  
Halidu Shafiu Kilishi ◽  
Fajobi Enobong Aloysious ◽  
Joshua Deji Abiodun
Keyword(s):  
Pseudomonas Fluorescens ◽  
Aqueous Extract ◽  
Aeromonas Hydrophila ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Tropical Fish ◽  
Pistia Stratiotes ◽  
Significant Activity ◽  
Water Lettuce ◽  
Fish Pathogens

Background: The study was undertaken to determine the phytochemical screening and the antibacterial activities of Pistia stratiotes (water lettuce) against some clinically isolated fish pathogens. Methods: The healthy and disease free plants samples were collected from Jebba Upper Basin of River Niger and tested for their antibacterial activities and phytochemical properties. Pistia stratiotes aqueous extract was extracted using sterile distilled water. The Agar diffused methods were used to determine the antimicrobial actions of the plant against fish pathogens of Pseudomonas fluorescens, Escherichia coli, Salmonella typhi, Aeromonas hydrophila, and Vibrio anguillarum. Results: The antibacterial investigation of aqueous extracts revealed the significant activity against these bacterial. Moreover, phytochemical examination of the crude extract showed the occurrence of saponins, alkaloids, tannins, anthraquinones, flavonoids, and catechins, which completely inhibited the growth of V. anguillarum and E. coli. However, Salmonella typhi, Pseudomonas fluorescens, and Aeromonas hydrophila exhibited moderate varying levels of resistance. Conclusion: The finding of this study showed that bioactive resistance from Pistia stratiotes can help disrupt the activities of various fish bacterial in aquaculture practices

scholarly journals In-Vitro Evaluation of Antimicrobial Activities of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Neisseria gonorrhoeae, and Candida albicans Nosodes

Homeopathy ◽  
2021 ◽  
Author(s):  
Renuka Munshi ◽  
Gitanjali Talele ◽  
Rajesh Shah
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
E Coli ◽  
Inhibition Of Growth

Abstract Background This study presents the results of the minimum inhibitory concentration (MIC) assay of a series of nosodes: namely Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Neisseria gonorrhoeae, and Candida albicans. Each was tested against its corresponding infection as well as cross infections. Methods In-vitro efficacy of polyvalent nosodes was tested using the MIC assay technique. The nosodes, namely C. albicans polyvalent nosode (35c, 100c), N. gonorrhoeae (35c), K. pneumoniae (35c, 100c), E. coli polyvalent nosode (35c, 100c) and Salmonella typhi polyvalent nosode (30c, 100c), were tested along with positive and negative controls. Nosodes were studied in different potencies and at 1:1 dilution. Results C. albicans polyvalent nosode 35c, 100c, N. gonorrhoeae 35c, and positive control amphotericin B showed inhibition of the growth of C. albicans species. K. pneumoniae 35c, E. coli polyvalent nosode 100c, and meropenem (positive control) showed inhibition of the growth of K. pneumoniae; this effect was not seen with ceftriaxone, ofloxacin and amoxicillin antibiotics. E. coli polyvalent nosode 30c in 10% alcohol (direct and dilution 1:1) and the positive controls ciprofloxacin, ofloxacin, and amoxicillin showed inhibition of the growth of E. coli. The S. typhi polyvalent nosode 30c in 10% alcohol showed inhibition of growth of S. typhi. Conclusion This study reveals that the tested nosodes exhibited antibacterial potential against the corresponding micro-organisms and against other selected organisms studied using this assay.

scholarly journals In-vitro Antimicrobial Susceptibility and Phytochemical Constituents of Methanol Leaf Extract of Prosopis africana against Some Selected Microorganisms

2019 ◽  
pp. 1-8
Author(s):  
Abdulmumin Adam Raji ◽  
Kassim Zainab Jummai ◽  
Mohammed Shehu Busu ◽  
Yakubu Ya’aba ◽  
Ibrahim Kolo
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Streptococcus Pyogenes ◽  
Leaf Extract ◽  
Salmonella Typhi ◽  
Human Beings ◽  
Phytochemical Screening ◽  
Prosopis Africana ◽  
Antimicrobial Potency

Introduction: The idea that certain plants had healing potential was known long before human beings discovered the existence of pathogens. Methodology: The crude methanolic leaf extract of Prosopis africana was assayed for antimicrobial potency using Agar-well diffusion technique against Salmonella typhi, Klebsiella pneumoniae, Streptococcus pyogenes, Pseudomonas aeruginosa, Methicillin-Resistant Staphylococcus aureus (MRSA) and Candida albicans. Qualitative phytochemical screening was also carried out. Results: The results of the antimicrobial screening showed antimicrobial potency against the test isolates with various degrees of zone of inhibition which varied between 10 mm – 22 mm. The highest zone was reported against Klebsiella pneumoniae (22 mm), followed by Streptococcus pyogenes and Candida albicans (21 mm), Salmonella typhi (20 mm), MRSA (19 mm) and then Pseudomonas aeruginosa (18 mm). Chloramphenicol and Fluconazole are used as reference standard and their zones of inhibitions ranged from 26 mm – 29 mm. The Minimum Inhibitory Concentration (MIC) of the extract ranged between 12.50 mg/mL – 50.00 mg/mL whilst the Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) of the extract were at 50.00 mg/mL. The result of phytochemical screening revealed the presence of carbohydrates, flavonoids, phenols/tannins, saponins, terpenes, steroids, cardiac glycosides and alkaloids as secondary metabolites. Conclusion: The findings clearly showed that methanolic leaf extract of P. africana has proved its use in folklore as an alternative antimicrobial agent and further research can lead to isolation of a new lead of medical importance.

Preparation and Standardization of Nosodes Sourced from Klebsiella Pneumoniae, Salmonella Typhi, Neisseria Gonorrhoeae and Candida Albicans Strains

Homeopathy ◽  
2021 ◽  
Author(s):  
Renuka Munshi ◽  
Gitanjali Talele ◽  
Rajesh Shah
Keyword(s):  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Material Culture ◽  
Therapeutic Potential ◽  
Biological Effects ◽  
Salmonella Typhi ◽  
Molecular Testing ◽  
Test Results ◽  
Bacterial Strains

Abstract Background Homeopathic nosodes prepared from organisms and pathological tissues have shown biological effects, encouraging more research. There is a need to develop some new nosodes systematically and to re-make others that were developed over a century ago. In our program of work on nosodes, the bacterial strains Klebsiella pneumoniae (BAA 2146), Salmonella typhi and Neisseria gonorrhoeae (ATCC 43069), and the single-celled fungus Candida albicans (24433, 26790, and 60193) have been identified for preparation. Materials and Methods The systematic and scientific method of preparation of nosodes includes identification, culture, quantification, characterization, preparation, and standardization. Under laminar flow, a suspension of respective bacterial and fungal cells (20 billion cells/mL) was processed as per the Homoeopathic Pharmacopoeia of India (HPI). Culture tests, sterility tests and molecular testing (polymerase chain reaction) were performed to establish the absence of contamination, live organisms and DNA material. Results K. pneumoniae, S. typhi (single, bivalent, or polyvalent), N. gonorrhoeae, and C. albicans nosodes (single and polyvalent) were sourced and prepared from different strains of respective cultures. The nosode preparations were processed by serial dilution and potentization, normally following the HPI guidelines. Molecular test results showed the absence of live organisms or DNA material; culture and sterility test results demonstrated the safety profile of the potentized nosodes. Conclusion K. pneumoniae, S. typhi, N. gonorrhoeae and C. albicans nosodes were successfully prepared. Their therapeutic potential may now be evaluated.

scholarly journals The Antimicrobial Activity of Extracts of the Lichen Hypogymnia tubulosa and Its 3-Hydroxyphysodic Acid Constituent

2005 ◽  
Vol 60 (1-2) ◽  
pp. 35-38 ◽  
Author(s):  
Meral Yılmaz ◽  
Turgay Tay ◽  
Merih Kıvanç ◽  
Hayrettin Türk ◽  
Ayşen Özdemir Türk
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Klebsiella Pneumoniae ◽  
Aeromonas Hydrophila ◽  
Proteus Vulgaris ◽  
Ethanol Extracts

The antimicrobial activity and the MIC values of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen Hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent have been investigated against some microorganisms. At least one of the extracts or 3-hydroxyphysodic acid showed antimicrobial activity against Aeromonas hydrophila, Bacillus cereus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Listeria monocytogenes, Proteus vulgaris, Salmonella typhimurium, Staphylococcus aureus, Streptococcus faecalis, and Candida albicans. No antifungal activity of the extracts has been observed against ten filamentous fungi.

In vitro Evaluation of the Antimicrobial activity of Methanolic and Aqueous extract of Mucuna pruriens seed

2021 ◽  
pp. 4212-4214
Author(s):  
Amita Shobha Rao ◽  
Shobha K L ◽  
Preethi Lavina Concessao ◽  
Gowrish Rao S ◽  
Sreedhara R Pai K
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Mucuna Pruriens ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Clinical Strains

The present investigation was to find out efficacy of Mucuna pruriens (M. pruriens) seeds. The antimicrobial activity of methanol and aqueous extract of M. pruriens seeds was determined by well diffusion method with ATCC strains of gram positive and gram negative bacteria clinical strains of multidrug resistant (MDR) Klebsiella pneumoniae, and clinical strains of Candida albicans. It was observed that both the aqueous and the methanol extract of M. pruriens seeds had antibacterial activity against all the ATCC bacterial strains. The MDR strain of Klebsiella pneumoniae was resistant to both aqueous and methanol extract of M. pruriens seed. Antifungal activity against Candida albicans was exhibited by both aqueous and methanol extracts of M. pruriens seed.

scholarly journals Effect of Roselle Hibiscus sabdariffah extract on some pathogenic microorganism

2011 ◽  
Vol 5 (3) ◽  
pp. 24-31
Author(s):  
Lamees M. R. Abbas ◽  
Laith A. Yaaqoub ◽  
Sabah Mahdie
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Plant Extract ◽  
Minimum Bactericidal Concentration ◽  
Salmonella Typhi ◽  
Inhibition Activity ◽  
Pathogenic Microorganism ◽  
Proteus Vulgaris ◽  
Inhibition Concentration ◽  
Mic Value

The inhibition activity for the aqueous and alcoholic extracts of Hibiscus sabdariffah being studied by the wells method on some microorganisms such as Staphelococcus aureous, Streptococcus epidermidis, Serratia marcesens, Proteus vulgaris, Enterococcus fecalus, Klebsiella sp, Candida albicans, Asparaglas niger. Escherichia coli, Salmonella typhi, The results also revealed that, the aqueous and alcoholic extracts of Hibiscus sabdariffah had inhibition effects for all microorganism, the cold aqueous showed the higher inhibition than the hot and alcoholic extracts of the plant on Serratia marcesens, Escherichia coli, Enterococcus fecalus at concentration (25,50,75,100)%, The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the plant extract. The results showed that the (MIC) value of all plant extract were (10, 2.5) % on Serratia marcesens, Escherichia coli respectively, were the value of (MBC) was (1.25, 5) % for the same bacteria

scholarly journals PENGUJIAN AKTIVITAS ANTIMIKROBA EKSTRAK ETIL ASETAT DAN METANOL KAYU RANTING SENGON (Falcataria moluccana) SAKIT

2021 ◽  
Vol 2 (1) ◽  
pp. 55-67
Author(s):  
Popy Listiani ◽  
Popy Hasanah ◽  
Alfi Rumidatul ◽  
Feldha Fadhila ◽  
Yayan Maryana
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Salmonella Typhi ◽  
Shigella Dysenteriae ◽  
E Coli

Klebsiella pneumoniae, Shigella dysenteriae, Salmonella typhi, Escherichia coli, dan Candida albicans merupakan mikroorganisme yang paling sering menginfeksi di Indonesia. Penyakit infeksi dapat disembuhkan serta diminimalkan penularannya menggunakan antibiotik. Upaya pencarian senyawa antimikroba dari alam diharapkan mampu mengurangi pengaruh negatif antibiotik. Tanaman yang diduga memiliki kandungan antimikroba salah satunya pohon sengon. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak kayu ranting sengon (Falcataria moluccana) yang terinfeksi jamur U. tepperianum dengan pelarut etil asetat dan metanol sebagai antimikroba. Metode pengujian yang digunakan Kirby bauer dengan perlakuan 9%, 9.5%, 10%, 10.5%, dan 11%. Hasil penelitian ekstrak etil asetat kayu ranting sengon sakit menunjukkan adanya aktivitas antimikroba terhadap S. dysenteriae pada semua konsentrasi dan K. pneumoniae pada konsentrasi 11%, tidak ada aktivitas antimikroba terhadap E. coli. Ekstrak metanol kayu ranting sengon sakit menunjukkan adanya aktivitas antimikroba terhadap K. pneumoniae pada konsentrasi 10%, 10.5%, 11%, dan C. albicans pada semua konsentrasi, namun tidak menunjukkan aktivitas antimikroba terhadap E. coli, S. typhi, dan S. dysenteriae. Dapat disimpulkan bahwa ekstrak etil asetat kayu ranting sengon sakit dapat menghambat pertumbuhan S. dysenteriae dan K. pneumoniae. Ekstrak metanol kayu ranting sengon sakit dapat menghambat pertumbuhan K. pneumoniae dan C. albicans.

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