scholarly journals Histopathological study of the Influence of Estradiol on Liver Regeneration in Male Rabbit

2013 ◽  
Vol 37 (2) ◽  
pp. 211-217
Author(s):  
Mahdi A. K.
Keyword(s):  
Liver Regeneration ◽  
Intramuscular Injection ◽  
Inflammatory Cells ◽  
Liver Parenchyma ◽  
Necrotic Area ◽  
Control Group ◽  
Histopathological Study ◽  
Central Vein ◽  
Generative Capacity ◽  
Post Operation

This study was investigated to recognize the generative capacity of liver that influenced by administration of estradiole benzoate. Twenty four healthy local breed male rabbits weighed 1.5-2 kg were used .They were classified into two equal groups control and treated groups. Animals anaesthetized by intramuscular injection of Ketamine (35mg /kg B.W) and Xylazine (5mg\kg B.W.) In control group partial hepatectomy of liver was done, while in treated animals were subject to a same operation then received an intramuscular injection 0.1ml of estradiol benzoate 0.2% at 3rd and 5th days post operation. At 3rd days microscopic finding showed biopsy were taken at 3rd, 7th and 14 day, post operation to study the histopathological changes in liver regeneration .At 3rd day it showed early proliferation of hepatocyte character by irregular hyperchromatic multinucleated cell .While in control group it showed necrotic area of liver parenchyma surrounded by thick fibrous connective tissue infiltrated with inflammatory cells. At 7th day in treated animals still progress in proliferation of hepatocyte while, liver proliferation phase showed in control group .At 14th day in treated animals showed large cells of hepatocyte with double nuclei aggregation without central vein , while in control group showed small hyperchromatic hepatocyte with present of necrotic area. In conclusion the results of this study indicate that administration of estrogen after partial resection in male rabbits has a role in liver regeneration by early initiate or facilitate heypatocyte proliferation.

Pathology of Pullorum Disease and Molecular Characterization of Its Pthogen

2020 ◽  
Vol 23 (1) ◽  
pp. 25-35
Author(s):  
MM Rahman ◽  
J Alam ◽  
MM Rahman ◽  
MAHNA Khan ◽  
MG Haider
Keyword(s):  
Molecular Characterization ◽  
Molecular Identification ◽  
Intestinal Mucosa ◽  
Inflammatory Cells ◽  
Liver Parenchyma ◽  
Histopathological Study ◽  
Isolation And Identification ◽  
Focal Necrosis ◽  
Salmonella Pullorum

This experiment was conducted to determine the pathology of pullorum disease of chickens and molecular identification of its pathogen. A total of 108 samples, including swabs from different organs were collected from different commercial layer farms of Gazipur district. The histopathological samples were collected in 10% formalin and the swabs were collected in tetrathionate broth. Samples were subjected to isolation and identification of the causal agent followed by gross and histopathological study of the affected visceral organs. Fifty eight out of 108 cloacal swabs (53.7%) were Salmonella positive. The percentage of Salmonella positive in liver swabs, spleen swabs, lung swabs and intestinal swabs from dead birds were 55.88%, 32.35%, 35.29% and 47.05%, respectively. On average, 52.94% livers of Salmonella affected birds were enlarged, congested and hemorrhagic and necrotic foci was present in 32.35% liver. Unabsorbed and coagulated yolk was found in 70.58% cases. From these 38.24% spleens were swollen and congested and 44.12 % kidneys were enlarged. At histopathology, 52.94% livers showed congestion, focal necrosis with multifocal infiltration of histiocytes in liver parenchyma. Focal necrosis and inflammatory cells were found in 70.58% spleen. Infiltration of heterophils in intestinal mucosa was found in 47.05% cases. 20.58% (7 out of 34) samples were PCR positive for Salmonella Pullorum organism. Ann. Bangladesh Agric. (2019) 23(1) : 25-35

Beneficial effects of rosmarinic acid against alcohol-induced hepatotoxicity in rats

2018 ◽  
Vol 96 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Parisa Hasanein ◽  
Rosa Seifi
Keyword(s):  
Rosmarinic Acid ◽  
Biological Activities ◽  
Inflammatory Cells ◽  
Liver Parenchyma ◽  
Serum Levels ◽  
Control Group ◽  
Ethanol Group ◽  
Beneficial Effects ◽  
Tnf Α ◽  
Hepatic Lipid Peroxidation

Alcohol is a severe hepatotoxicant that causes a variety of liver disorders. Rosmarinic acid (RA), a natural phenol, shows some biological activities, including antioxidant and anti-inflammatory effects. We investigated the effects of RA (10 mg/kg) against ethanol-induced oxidative damage and hepatotoxicity in rats. Animals received ethanol (4 g/kg, i.g.) and (or) RA (10 mg/kg, i.g.) daily for 4 weeks. At the end of the treatment period, rats were weighed and use for biochemical, molecular, and histopathological examinations. Ethanol increased hepatic lipid peroxidation (P < 0.001) and decreased hepatic levels of reduced glutathione (P < 0.01), catalase (P < 0.05), and superoxide dismutase (P < 0.001) compared with control group. RA prevented the prooxidant and antioxidant imbalance induced by ethanol in liver. Furthermore, RA ameliorated the increased liver mass, serum levels of ALT, AST, LDH, TNF-α, and IL-6 in ethanol group. Necrosis and infiltration of inflammatory cells in liver parenchyma were attenuated by RA treatment. Our findings showed that RA prevents ethanol-induced oxidant/antioxidant imbalance and liver injury in an experimental model of ethanol-induced hepatotoxicity. Therefore, RA may be a good candidate to protect against ethanol-induced hepatotoxicity; this deserves consideration and further examination.

scholarly journals Study of some toxicological aspects of Alfa-cypermethrin in rabbits

2010 ◽  
Vol 9 (3) ◽  
pp. 82
Author(s):  
Dh. R.H. Al-Fetly And N. K. M. Al-Nakeeb
Keyword(s):  
Biochemical Study ◽  
Liver Parenchyma ◽  
Blood Parameters ◽  
Bile Secretion ◽  
Control Group ◽  
Histopathological Study ◽  
Dependent Manner ◽  
Petechial Hemorrhage ◽  
Hb Concentration ◽  
And Control

This study was conducted to evaluate the immediate alterations that occur in rabbitsafter acute toxicity with single oral dose of cypermethrin. Twenty one rabbits were randomlydivided into three equal groups: treated groups (A1), (A2) and control group used in thisstudy. Groups (A1), (A2) received orally cypermethrin in dose of (25, 50 mg/kg body weight)respectively, While control group received normal saline at same time. Most obvious clinicalsings in the treated groups were Restlessness, salivation, licking of legs and face, frequenturination, muscular tremor, incoordination, and ataxia appeared in dose dependent manner. Inaddition, significantly increased in respiratory rate and heart rate in treated groups comparedwith control group and appeared petechial hemorrhage and congestion in the conjunctiva intreated groups. The blood samples were taking to evaluate some of blood parameters includes(RBC counts, WBC counts, PLT counts, Hb concentration, PCV, RBCs indices (MCV, MCH,MCHC, RDW -CV and RDW - SD), PDW, MPV, P-LCR and some biochemical study asALT and AST. Two rabbits from each group were killed to evaluate the histopathologicalalteration in liver. The statistical analysis revealed that there were significant decreased inRBC counts and Hb concentration (P<0.05) in treated groups (A1), (A2) as compared withcontrol group. The PCV percentage, platelet counts and MCHC values revealed significantdecreased (P<0.05) between group (A2) and control group. The RDW-SD, RDW-CV valveswere significantly increased (p<0.05) between group (A1) and control group. Other parameterstudied did not show significant differences between treated groups and control group. Thestatistical analysis revealed that the levels of ALT and AST were significantly elevated(P<0.05) in (A1 and A2) groups as compared with control group. In gross appearance therewere petechial hemorrhage and erosion of liver parenchyma. Histopathological study revealedthat there were engorgement of hepatic vein with presence of extravasated RBCs between thehepatocytes in group (A1), while group (A2) the hepatocytes undergo atrophy and severenecrosis and destruction with infiltration of polymorphnuclear leukocytes and engorgement ofsome portal trait with bile secretion

scholarly journals The effectiveness of Pectin in the reduction of histological changes caused by exposure to monosodium glutamate in female mice: مدى فعالية البكتين في الحد من التغيرات النسيجية التي يسببها تناول جلوتامات أحادي الصوديوم على إناث الفئران

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Sarah Ibrahim Al Othman, Faten khalif Alanazi, Ghada Jaber S
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Monosodium Glutamate ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Control Group ◽  
Central Vein ◽  
Histological Changes ◽  
Female Mice ◽  
Liver And Kidney

Monosodium glutamate (MSG) is widely used as a food additive. Excessive consumption of monosodium glutamate has also been shown to affect the liver and kidneys, causing damage to these tissues because of oxidative stress leading to increased production of reactive oxygen species (ROS). The purpose of the study described in this paper was to find out how the liver and kidney toxicity caused by monosodium glutamate can be mitigated using pectin. To this end, 30 albino mice females were divided into four groups. The animals were distributed in special cages. 12-15 weeks with an average body weight of 60 grams. The animals were divided into four groups: the experimental control group (1) comprising 5 female mice were given normal drinking water and the treated group (2) comprising 10 female mice were given monosodium glutamate at a dose of 3 g/kg body weight in drinking water. For three weeks, the treatment group (3) comprising 10 female mice was given pectin at a dose of 300 mg/70 kg body weight in drinking water immediately after the monosodium glutamate dose for three weeks and the pectin group (4) comprising 5 female mice were given Pectin at a dose of 300 mg/70 kg body weight in drinking water for three weeks. The mice were then anesthetized, dissected, and liver and kidney samples were taken from female mice and kept in a 10% neutral formalin solution to make tissue segments. The results showed many histological changes in the liver, such as congestion of the central vein, widening of the sinuses, and the appearance of signs of the death of most hepatocytes, infiltration of the central vein and an invasion of inflammatory cells around the central vein with the emergence of several gaps within the cells. Many of them cavity with the death of most of the tubule cells, the closure of some of them and the expansion and infiltration in others and bleeding inside the tissue. Pectin therapy has led to the disappearance of most of these changes and the emergence of a clear improvement in hepatic and renal tissue.

scholarly journals THE EFFECT OF PRETREATMENT WITH TOLL-LIKE RECEPTOR 4 ANTAGONIST RESATORVID ON METHOTREXATE-INDUCED LIVER INJURY IN RATS: HISTOPATHOLOGICAL STUDY

2018 ◽  
Vol 11 (12) ◽  
pp. 560
Author(s):  
Bassim I Mohammad ◽  
Bassim S Ahmed ◽  
Alaa F Hassan ◽  
Samer F Hassan
Keyword(s):  
Liver Tissue ◽  
Inflammatory Cells ◽  
Fatty Degeneration ◽  
Treated Group ◽  
Control Group ◽  
Histopathological Study ◽  
Tissue Samples ◽  
Alcoholic Fatty Liver ◽  
Tissue Sections ◽  
Microscopic Evaluation

Objective: This research aims to evaluate the histopathological changes after pretreatment with resatorvid against methotrexate induced-liver injury.Methods: 28 male albino-wistar rats divided into random 4 groups (7 rats in each). Control group: Rats left untreated. Vehicle pre-treated group: Rats were administered dimethyl sulfoxide (DMSO) followed by methotrexate (MTX). Methotrexate treated group: Rats left untreated then administered MTX. Resatorvid pre-treated group: Rats were administered resatorvid followed by MTX. 24 h after the end of treatment, the animals were sacrificed. Liver tissue samples dissected out immediately and fixed in 10% formalin. The traditional procedures (paraffin-embedded method) was used to prepare liver tissue for microscopic evaluation by none alcoholic fatty liver disease (NAFLD) Activity Score Components.Results: Liver tissue sections of MTX-treated group show moderate-to-severe steatosis of hepatic cells and micro- and macro- hepatocellular fatty degeneration and giant fatty cysts with chronic inflammatory cells infiltration. While liver tissue sections of the resatorvid pre-treated group show moderate hepatic cellular fatty degeneration, with a decreased number of fatty cysts chains and the inflammation disappeared.Conclusion: Resatorvid hepatoprotective effect against MTX-induced injury was promising throughout resolving the accompanying inflammation and partial restoring histopathological fatty alterations.

scholarly journals Histopathological and Biochemical study of the effect of Citrullus colocynthis on the heart and liver of mature male rabbits: as a model for mammals

2012 ◽  
Vol 36 (0E) ◽  
pp. 322-330
Author(s):  
Saad S. Al-Dujaily
Keyword(s):  
Biochemical Study ◽  
Portal Tract ◽  
Inflammatory Cells ◽  
Mature Male ◽  
Control Group ◽  
Histopathological Study ◽  
Histopathological Changes ◽  
Citrullus Colocynthis ◽  
Serum Glutamate ◽  
Low Dosage

The aim of the present work is to found out if there are histopathological and enzymatic effects on the heart and liver after oral administration of Citrullus colocynthis (CC) fruit (bitter apple) for mature male rabbits, as a model for mammals. Two experiments were performed for sixty mature male rabbits that equally and randomly divided into six groups each containing five animals .In the two experiments ,independently, three groups in each one were considered as treated groups and represented as T1, T2 and T3 groups .These rabbits groups were orally administrated with low dosage of CC (4.8mg/kg/day,experiment No.1 ) and double dosage of CC extract (9.6mg /kg /day , experiment No2 ) for three intervals 2, 4 and 8 weeks ,respectively. The other three groups in each experiment were considered control and represented as C1, C2 and C3 groups, respectively. All the rabbits in control groups were given orally distilled water (DW) at the same periods of treated groups. Histopathological changes of the heart and liver with related enzymes namely; serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and creatinine kinase (SCK) were studied.The histopathological study revealed no changes in rabbit’s heart tissue particularly after treatment with 4.8mg/kg/day of CC for 2 and 4 weeks. While no histopathological changes were observed in the heart of rabbit groups treated with 9.6mg/kg/day of CC extract after 2 weeks. The results of the treatment with the two dosages of the CC for 8 weeks indicated a mild degenerative changes and mild necrosis of the myocardial cells. There was swelling of the hepatocytes and perivascular cuffing of mononuclear inflammatory cells after two weeks of daily treatment with 4.8mg/kg/day of CC exposure. After four weeks with the low dosage of the CC extract, caused initiation of necrosis, more inflammation picture of liver portal tract with sinusoid All rabbit groups showed statistically a significant gradual increase (P<0.05) in the value of serum enzyme GOT, GPT and CK levels after treatment with the two dosages of CC at the end of each different period compared with before treatment and control group. Concluded from this study that the dosage 4.8mg/Kg/day of CC plant resulted in simple histopathological effects on the heart and mild histopathological changes on the liver during the entire period of the study, instead of serum enzymatic elevation of SGOT ,SGPT and SCK. Further studies are recommended to found out the possibility to use and effects of CC on animal hygiene and reproduction

scholarly journals THE PROTECTIVE ROLE OF FLAXSEED LIGNAN IN MALE RABBITS WITH HIGH-FAT DIET: HISTOPATHOLOGICAL STUDY

2016 ◽  
Vol 8 (11) ◽  
pp. 90
Author(s):  
Zahraa Ahmed Okhti ◽  
Muthanna I. Al-ezzi ◽  
Raghad Abdulmahdi
Keyword(s):  
Blood Vessels ◽  
Bile Ducts ◽  
Mononuclear Cells ◽  
Inflammatory Cells ◽  
Negative Control Group ◽  
Negative Control ◽  
Normal Diet ◽  
Control Group ◽  
Histopathological Study ◽  
Portal Area

Objective: The objective of the study was to investigate the effect of dietary flaxseed-derived lignan supplement as anti-hyperlipidemic (fatty liver changes) on rabbit liver.Methods: Rabbits were assigned randomly into three groups (five rabbits for each group): group-1, normal diet (negative control); group-2, 1.6 % cholesterol diet for 30 d (positive control) and group-3, 1.6 % cholesterol for 30 d then gave 40 mg/kg/day of pure flax lignan for 14 d.Results: Marked proliferation of fibrous connective tissue infiltrated with mononuclear cells was recorded; congested dilated of sinusoids and fibrosis in the portal area around the proliferation of bile ducts and congested portal blood vessels with the proteinous material in the lumen of bile ducts were also recorded. In other sections, the liver showed large necrotic area replacement with RBCs as well as enlarged foamy hepatocytes with mononuclear cells in narrowing sinusoids were seen.Conclusion: From the results of this study we can conclude that lignan has been used successfully in decreasing the inflammatory cells in congested blood vessels and sinusoids and has decreased the inflammatory cells infiltration in moderate fibrosis in the portal area around the bile ducts of rabbit liver tissue.

scholarly journals Prophylactic Effect of Ethanolic Extract of Saussurea Costus Roots Against Hepato- Renal Toxicity Induced by Diazinon in Chickens

2020 ◽  
Vol 13 (2) ◽  
pp. 34-51
Author(s):  
Alaa K. Hamad ◽  
Mariam Kadhem
Keyword(s):  
Morphological Changes ◽  
Clinical Signs ◽  
Ethanolic Extract ◽  
Inflammatory Cells ◽  
The Body ◽  
Control Group ◽  
Central Vein ◽  
Histopathological Changes ◽  
Group 2 ◽  
Group 1

The study was performed to investigate the prophylactic role of Saussurea costus roots in the functional and histological changes caused by diazinon in the liver and kidneysin chickens. 18 chickens were used of Arbor Acres strain at 54 days of age and 1300-1500 gm average of body weight was used for this purpose. The birds were divided into 3 groups equally (6chicken in each groups), Group 1: served as a control negative received distilled water 1 ml. Group 2: served as a positive control group, received diazinon at a dose of 0.2 mg/kg/day. Group 3: were given an ethanolic extractof Saussurea costus at dose 300 mg/kg, then after one hour administrated diazinon at the dose of 0.2 mg/kg/day, this group served as a prophylactic group. The doses were given orally once daily for 4 weeks.The results of group 2 showed clinical signs such as ruffled feathers, salivation, diarrhea, breathing from the mouth, teary eyes, drooping of wings. The body weights of the chickens and weights of the liver and kidneys of group 2 significantly declined (p≤ 0.05) compared with groups 1 and 3. There was a significant decrease (p≤ 0.05) in WBC count, lymphocyte, total protein, albumin, GSH, SOD, CAT, and GPX levels, while a significant increase (p≤0.05) in heterophil, ALT, AST, creatinine, urea, uric acid, and MDA compared with group 1. The gross examination of the liver and kidney of group 2 were pale, easily crumbles and smaller than that of group 1. Histopathological changes of the liver of group 2 including congested and dilated central vein, vacuolated cytoplasm of hepatocytes, focal necrotic tissue filled with inflammatory cells, thickening of the bile duct, thickening wall of the portal artery. fibroblast in portal area, dilated sinusoid. Histopathological changes of the kidney including dilatation of renal tubule, hemorrhage, and atrophy in the glomerulus. we concluded that administration of ethanolic extractof Saussurea costus resulted in amelioration of the morphological changes in diazinon treated chickens, improved parameters and restored the parameters to near normal compared with group 1. These results revealed that Saussurea costus roots acts as an antioxidant substance and has a hepatic and renoprotective effect against toxicity induced by diazinon.

scholarly journals The influence of estrogen on liver regeneration: an experimental study in rats

2009 ◽  
Vol 24 (1) ◽  
pp. 3-6 ◽  
Author(s):  
Maria de Lourdes Pessole Biondo-Simões ◽  
Thomas Rolf Erdmann ◽  
Sérgio Ossamu Ioshii ◽  
Jorge Eduardo Fouto Matias ◽  
Hugo Leonardo Guaita Calixto ◽  
...  
Keyword(s):  
Experimental Study ◽  
Liver Regeneration ◽  
Intramuscular Injection ◽  
Wistar Rats ◽  
Peanut Oil ◽  
Control Group ◽  
Regenerative Capacity ◽  
Female Wistar Rats ◽  
Group A ◽  
Made In

PURPOSE: To recognize the regenerative capacity influenced by the administrating of estradiol. METHODS: 42 female Wistar rats were used, divided into two groups, the control and the experiment group. A resection of approximately 70% of the liver was made in the liver of these animals. The control group received an intramuscular injection of one ml of peanut oil. The experiment group were given estradiol hexahydrobenzoate (50µg) diluted in one ml of peanut oil. Calibrations were done after 36 hours and 7 days, using three methods: the formula of Kwon et al.21, to recognize gain in volume, counting of the mitosis figures in five fields and the percentage of positive PCNA nuclei. RESULTS: Gain in volume (mass) was similar in both groups after 36 hours (p=0.1873) and higher in the experiment groups after seven days (p=0.0447). Microscopy showed a similar number of mitosis figures after 36 hours (p=0.3528) and a tendency to be higher in the experiment group after 7 days (p=0.0883). The average of positive PCNA nuclei was higher in the experiment group both after 36 hours (p=0.0009) and 7 days (p=0.0000). CONCLUSION: The estradiol hexahydrobenzoate improved liver regeneration in rats submitted to a 70% hepatectomy.

scholarly journals Mitigation of Radiation-Induced Lung Pneumonitis and Fibrosis Using Metformin and Melatonin: A Histopathological Study

Medicina ◽  
2019 ◽  
Vol 55 (8) ◽  
pp. 417 ◽  
Author(s):  
Farhood ◽  
Aliasgharzadeh ◽  
Amini ◽  
Rezaeyan ◽  
Tavassoli ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Radiation Pneumonitis ◽  
Inflammatory Cells ◽  
Control Group ◽  
Histopathological Study ◽  
High Dose ◽  
Antioxidant Agents ◽  
Radiation Induced ◽  
Group 2

Background and objectives: Pneumonitis and fibrosis are the most common consequences of lung exposure to a high dose of ionizing radiation during an accidental radiological or nuclear event, and may lead to death, after some months to years. So far, some anti-inflammatory and antioxidant agents have been used for mitigation of lung injury. In the present study, we aimed to detect possible mitigatory effects of melatonin and metformin on radiation-induced pneumonitis and lung fibrosis. Materials and methods: 40 male mice were divided into 4 groups (10 mice in each). For control group, mice did not receive radiation or drugs. In group 2, mice were irradiated to chest area with 18 Gy gamma rays. In groups 3 and 4, mice were first irradiated similar to group 2. After 24 h, treatment with melatonin as well as metformin began. Mice were sacrificed after 100 days for determination of mitigation of lung pneumonitis and fibrosis by melatonin or metformin. Results: Results showed that both melatonin and metformin are able to mitigate pneumonitis and fibrosis markers such as infiltration of inflammatory cells, edema, vascular and alveolar thickening, as well as collagen deposition. Conclusion: Melatonin and metformin may have some interesting properties for mitigation of radiation pneumonitis and fibrosis after an accidental radiation event.

Sign in / Sign up

Export Citation Format

Share Document