scholarly journals The effect of the microelement preparation on the morphobiochemical composition of the blood of calves

2021 ◽  
Vol 22 (3) ◽  
pp. 418-427
Author(s):  
S. V. Nikolaev
Keyword(s):  
Blood Cells ◽  
Saline Solution ◽  
White Blood Cells ◽  
Control Group ◽  
Intact Group ◽  
Stable Maintenance ◽  
Positive Effect ◽  
Experimental Group ◽  
Time Injection ◽  
Milk Feeding

The paper presents an assessment of the effect of the Sedimine microelement product on the morphobiochemical composition of the blood of calves during the milk feeding period. For the experiment, 2 groups of heifers of HolsteinKholmogory breed at the age of 2-3 weeks were formed. The calves of the experimental group were injected intramuscularly with Sedimine at a dose of 5 ml three times with an interval of 7 days, and the control animals were given a saline solution in the same way. Blood for hematological and biochemical studies was obtained at the beginning of the experiment and a week after the first and last injection. According to the research it has been established, that after a single injection of Sedimine, there were no evident changes in the biochemical profile of the blood, with the exception of a decrease in the level of urea by 30.6% (from 4.9 to 3.4 mmol/l; P<0.001). After three-time administration of the drug, there was an increase in total protein by 9.6 % (from 54.2 to 59.4 g / l; P<0.05) and an increase in AlAT activity by 1.2 times (P<0.001), while in comparison with the control, the activity of AsAT was greater (P<0.01) by 43.4 % (57.5 U/l). The de Ritis coefficient in the experimental group decreased by 43.1 % (to 6.2; P<0.01), while in the control group, on the contrary, the indicator increased by 32.1 % (to 11.1). The ratio of calcium to phosphorus in all animals decreased by 28.8...32.1 % (P<0.001), but in young animals treated with Sedimine, the ratio was 16.7 % (0.84) higher compared to the intact group of calves (P<0.05). The concentration of thyroglobulin in the experimental group significantly decreased by 29.4 % (from 0.34 to 0.24; P<0.01), with stable values in calves that were treated with saline. Injections of the preparation contributed to the stable maintenance of iron in the blood of animals of the experimental group, the concentration of which was 3 times higher (P<0.01) in relation to the control, where the indicator was 5.9 micromol/l. Also, the control animals showed a decrease in the number of red blood cells by 31.9% (from 4.7 * 10 12 to 3.2*10 12) and hemoglobin by 19.5% (from 87.2 to 70.2 g / l; P<0.05), with stable values in the experimental group, which were higher by 56.3 % (5.0*1012) and 22.2 % (90.2 g/l) compared to the control (P<0.05). Changes in white blood were characterized by a higher level of white blood cells in calves of the experimental group (by 27.7 %), primarily due to a 36.7 % (4.1*109 ; Р<0.01) higher number of lymphocytic cells. Thus, a three-time injection of the test drug was quite effective in preventing iron deficiency in calves during milk feeding and had a positive effect on metabolic processes.

Effect of miR-155 on Aplastic Anemia Rats Through Regulating Signal Transducer and Activator of Transcription 3 Signaling Pathway

2020 ◽  
Vol 10 (3) ◽  
pp. 397-401
Author(s):  
Qingxian Yan ◽  
Zhengjun Hou ◽  
Shuting Ye ◽  
Meiyun Su
Keyword(s):  
Aplastic Anemia ◽  
Signaling Pathway ◽  
Blood Cells ◽  
Mrna Level ◽  
White Blood Cells ◽  
Control Group ◽  
Stat3 Signaling ◽  
Group I ◽  
Stat3 Signaling Pathway ◽  
Experimental Group

Objective: To assess miR-155’s effect on aplastic anemia (AA) rats. Methods: In the present study, the healthy rats (control group) and AA rats including AA rats with miR-155 overexpression (experimental group I) and those with miR-155 deficiency (experimental group II), were selected. The levels of miR-155, STAT3 (a key gene in STAT3 signaling pathway) and phosphorylated STAT3 (p-STAT3) in control group and experimental group were detected via qPCR and Western blotting. Moreover, the number of white blood cells (WBCs), red blood cells (RBCs), platelets (PLTs) and hemoglobin (HGB) level were also measured. Results: The level of miR-155 in AA rats was significantly declined compared with that in healthy rats (p < 0.05). STAT3 mRNA level was significantly declined in AA rats with miR-155 overexpression compared with that in AA rats with miR-155 deficiency (p < 0.05). STAT3 and p-STAT3 protein expression in AA rats with miR-155 overexpression were significantly lower than those in AA rats with miR-155 deficiency (p < 0.05). Besides, it was found that the number of WBC, RBC, PLT and HGB level were significantly elevated in AA rats with miR-155 overexpression compared to those in AA rats with miR-155 deficiency (p < 0.05). Conclusion: miR-155 can improve the AA symptoms of rats through inhibiting the STAT3 signaling pathway.

scholarly journals Tartrazine induces structural and functional aberrations and genotoxic effectsin vivo

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3041 ◽  
Author(s):  
Latifa Khayyat ◽  
Amina Essawy ◽  
Jehan Sorour ◽  
Ahmed Soffar
Keyword(s):  
Comet Assay ◽  
Blood Cells ◽  
White Blood Cells ◽  
Pharmaceutical Products ◽  
Food Additive ◽  
Control Group ◽  
Adverse Health Effects ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Experimental Group

Tartrazine is a synthetic organic azo dye widely used in food and pharmaceutical products. The current study aimed to evaluate the possible adverse effect of this coloring food additive on renal and hepatic structures and functions. Also, the genotoxic potential of tartrazine on white blood cells was investigated using comet assay. Twenty adult male Wistar rats were grouped into two groups of 10 each, control- and tartrazine-treated groups. The control group was administered orally with water alone. The experimental group was administered orally with tartrazine (7.5 mg/kg, b.wt.). Our results showed a marked increase in the levels of ALT, AST, ALP, urea, uric acid, creatinine, MDA and NO, and a decreased level of total antioxidants in the serum of rats dosed with tartrazine compared to controls. On the other hand, administration of tartrazine was associated with severe histopathological and cellular alterations of rat liver and kidney tissues and induced DNA damage in leucocytes as detected by comet assay. Taken together, the results showed that tartrazine intake may lead to adverse health effects.

scholarly journals Hematological Importance of Pseudoeosinophilic Granulocytes in Acclimation of Common Carp (Cyprinus Carpio Linnaeus, 1758)

2017 ◽  
Vol 40 (1) ◽  
pp. 5-11
Author(s):  
Damir Suljević ◽  
Adelaida Martinović-Jukić ◽  
Muhamed Fočak ◽  
Andi Alijagić ◽  
Dunja Rukavina ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Water Temperature ◽  
Cyprinus Carpio ◽  
Blood Cells ◽  
White Blood Cells ◽  
Control Group ◽  
Adaptation Mechanisms ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Experimental Group

Abstract Adaptation mechanisms as response to water content, oxygen level and pollutants are very important and they can be interpreted by hematological analysis. The aim of this study was the analysis of hematological and immune adaptations of common carp (Cyprinus carpio Linnaeus, 1758) to thermal stress. All specimens were divided into a control and experimental group. The control group of fish was exposed to a constant water temperature of 10°C. We induced thermal stress in experimental fish by gradually heating water to 28°C, held for 30 minutes and then comparing the obtained results with the control fish. Short-term hyperthermia lead to an increase of the number of leukocytes, especially pseudoeosinophilic granulocytes and monocytes, while the number of neutrophils and lymphocytes was reduced. The analysis of the leukocyte number and differential blood count in the control group showed high individual variation of segmented granulocytes, monocytes and pseudoeosinophilic granulocytes. Statistically significant differences (p=0.00) were found for the white blood cells, nonsegmented neutrophils and pseudoeosinophils between the control and experimental group. The experimental group of males had an increased number of white blood cells, monocytes and pseudoeosinophils, where significant differences were found for nonsegmented and total neutrophils and also for pseudoeosinophils (p=0.00), lymphocytes (p=0.01) and monocytes (p=0.03). Females had an increased total number of white blood cells, lymphocytes, monocytes and pseudoeosinophils, while significant differences (p=0.00) were obtained in the number of white blood cells, nonsegmented and total neutrophils and pseudoeosinophils between the control and experimental group. Adaptation mechanisms in carp after water temperature heating are mostly reflected in the increase of pseudoeosinophils and the decrease of neutrophils.

HEMATOLOGICAL INDICATORS OF CALVES IN THE PREVENTION OF HYPOELEMENTOSIS IN THE AMUR REGION

2020 ◽  
pp. 148-153
Author(s):  
N. N. Malkova ◽  
◽  
М. Е. Ostyakova ◽  
S.А. Sherbinina ◽  
◽  
...  
Keyword(s):  
Blood Cells ◽  
Color Index ◽  
White Blood Cells ◽  
Experimental Period ◽  
Amur Region ◽  
Hemoglobin Level ◽  
Blood Oxygenation ◽  
Control Group ◽  
Hematological Indicators ◽  
Experimental Group

Hematological studies were conducted in calves in dynamics during the prophylaxis of hypo-elementosis in the Amur region. The object of the study was calves (breed-Holstein, age-up to two months). The selected animals were divided into two equivalent groups: control and experimental. In the control group, planned measures were taken to prevent hypo - elementosis; in the experimental group, complex injectable drugs were used: Se-containing and tissue. Laboratory tests of calves ‘ blood were performed at the beginning and end of the experiment with determining the number of red blood cells, white blood cells, hemoglobin level, calculating the color index, differential counting of white blood cells and calculating the color index. It was established that the quality of blood oxygenation in calves of the experimental group improved. This is confirmed by the regeration to the standard values of hemoglobin level and color index as a result of their growth by 28 and 17%, respectively. At the end of the experimental period, the percentage of conditionally healthy calves was 80 % in the experimental group and 40 % in the control group. Thus, it should be noted that the proposed scheme of complex application of Se-containing and tissue preparations contributed to the normalization of erythropoiesis processes and increased the resistance of calves to diseases by 40 %.

Cellular Changes and Time since Death

1993 ◽  
Vol 33 (3) ◽  
pp. 213-222 ◽  
Author(s):  
C J Babapulle ◽  
N P K Jayasundera
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
White Cell ◽  
Control Group ◽  
Time Interval ◽  
Cell Counts ◽  
Total Differential ◽  
Hospital Patients ◽  
Nuclear Changes ◽  
Experimental Group

Based on a hypothesis that post-mortem cellular (chiefly nuclear) changes in the white blood cells could reliably be correlated with the time interval since death, (ti), serial observations were made on the counts (total, differential) and light-microscopically observable ‘degenerations’ of white blood cells obtained from 30 non-refrigerated cadavers (experimental group) and similar cells obtained from 200 hospital patients (control group). While neutrophils degenerated rapidly, lymphocytes did so slowly; the eosinophils and monocytes degenerated at rates between these extremes. In cadaveric blood total counts of identifiable leucocytes on average dropped to zero by 84 hours, identifiable eosinophils and monocytes were first to ‘disappear’ (by 60 hours), followed by neutrophils (by 66 hours), and finally lymphocytes: identifiable lymphocytes disappeared completely at or around 84 hours from the time of death. This ‘differential degeneration’ was surprising but useful. Based on the use of all four characteristics — total and differential white cell counts, differential degeneration and morphology of cells — a method for a reasonably exact estimation of ti is presented. The method is appropriate for ti up to 84 hrs (31/2 days). Zero white cell counts (total, differential) and bizarre morphology (unidentifiable white blood cells) indicate a ti > 84 hrs. Avenues for further research are indicated.

scholarly journals Effect of consumption of I, Se, S and nanoaquacitrates on hematological and biochemical parameters of the organism of rabbits

2021 ◽  
Vol 12 (2) ◽  
pp. 335-340
Author(s):  
О. V. Boiko ◽  
О. F. Honchar ◽  
Y. V. Lesyk ◽  
І. І. Kovalchuk ◽  
B. V. Gutyj ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Reproductive Function ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Limiting Factor ◽  
Group Iii ◽  
Mineral Substances ◽  
Preparation Period ◽  
Experimental Group

Using nanotechnologies to obtain compounds of mineral substances is promising because of the importance of the amount of mineral substances, but the limiting factor is particularly their bioavailability in the digestive tract. The conducted studies determined the stimulating effect of nanoaquachelates of separate biogene microelements on the course of biochemical processes of the organisms of animals, their productivity and quality of the obtained products. However, the mechanisms of impact of carboxylates of separate elements and their combinations on functioning of physiological systems of various species of animals, level of transformation of these elements into the products and their biological value remain unstudied. Therefore, the objectives of the study were changes in hematological biochemical and productive parameters of the organisms of rabbits consuming I, Se, S, the impacts of these elements on their organism from the 53 to 85th days of life. Studies were performed on young Termonde rabbits. Animals of experimental groups I, II, III, and IV were fed with feeds of the diet of the control group for 24 h with water containing solution of І, Se, S calculated respectively as 2.5, 5.0, 10.0 and 20.0 µg of І/L of water. The experiment lasted 45 days, including a 12 day preparation period and 33 day experimental. On the 53rd day of life of animals in the preparation period, and 68th and 85th days of life in the experimental periods (15th and 33rd days of watering with supplements), we collected samples of blood from the marginal ear vein of 6 animals (3 males and 3 females) of the group for hematological and biochemical studies. We determined that watering with the solution of microelements І, Se, S led to changes in the overall amount of white blood cells, making it 17.1% lower in the blood of animals of the experimental group II, 26.2% higher in group ІІІ on the 15th day, and 12.0% higher in group IV on the 31st day of the experiment compared with the control. Absolute amounts of lymphocytes, monocytes and granulocytes by the periods of the study were observed to have no significant changes throughout the study, though their parameters were within the physiological values compared with the control. The amount of red blood cells in the blood of rabbits of experimental groups І, ІІ and ІІІ was higher respectively by 8.1%, 5.7% and 12.0% at the first stage of the study and 18.1%, 13.3%, 24.5% and 24.9% higher in animals of experimental groups І, ІІ; ІІІ and ІV at the final stage of the study compared with the control group. Hemoglobin concentration in blood of rabbits of experimental groups II and III during the study was significantly higher. Hematocrit value in blood of rabbits of experimental groups I–III was significantly higher on the 33rd day of the experiment compared with the control. Consumption of solution of microelements in the amount of 10.0 µg of I/L by the animals of experimental group III led to 15.1% higher content of total protein in the blood on the 33rd day of the study compared with the control group of animals. The level of creatinine was no higher than the physiological parameters and was 9.2%, 15.0% and 15.4% higher in the blood of rabbits of experimental groups II, III, IV on the 33rd day of the experiment compared with the control group. No significant changes in the content of triacylglycerols in the blood of rabbits of the experimental groups were seen throughout the study. However, the content of cholesterol in the blood of rabbits of experimental groups III and IV was significantly higher at the first stage compared with the control group. Further, it is practical to study physiologically substantiated amounts of solution of microelements І, Se, S in the diet of mother rabbits to determine their effect on their ability to become pregnant, reproductive function, milk production and survival of offspring.

scholarly journals ЗМІНИ В КРОВІ ЩУРІВ ПІСЛЯ ІМУНІЗАЦІЇ ПРЕПАРАТОМ «ЛЕЙКОЗАВ» ПРОТИ ЛЕЙКОЗУ ВЕЛИКОЇ РОГАТОЇ ХУДОБИ

2016 ◽  
Vol 18 (3(70)) ◽  
pp. 103-108
Author(s):  
A.I. Zaviriukha ◽  
O.I. Vischu ◽  
H.A. Zaviriukha
Keyword(s):  
Adverse Effects ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Unit Volume ◽  
White Blood Cells ◽  
Statistical Error ◽  
Control Group ◽  
Blood Unit ◽  
Blood Eosinophils ◽  
Experimental Group

The article presents the results of research of hematological blood of rats under the influence of the drug «Leykozav» against bovine leukemia. Experienced animal drug «Leykozav» introduce twice at an interval of 14 days at 0.5 cm3.Before the introduction and after the introduction of studied hematologic parameters: the number of white blood cells, red blood cells and platelets and their indices, the hemoglobin content.According to the research found that the number of red blood cells, hemoglobin and hematocrit values are reduced to within statistical error, and no adverse effects on the health of laboratory animals.The introduction of the drug «Leykozav» rats did not cause any significant changes in terms of platelet indices in the blood of animals on the seventh day after the inoculation.The decrease number of segmented neutrophils and increase in the number of lymphocytes in the blood unit volume (1 at 14 and 30 days after inoculation (r < 0.05 – 0.01) increase the number of monocytes and 1,6 times at 30 days after immunization (r < 0,05) compared to before vaccination period.Influenced «Leykozav» preparation two weeks after immunization animals increases the amount of blood basophils (2 – 2,5 mkl). Compared with the control group of animals of the experimental group of blood eosinophils of rats indices are within physiological norms. 

STUDY OF RESISTANCE OF THE PIG BODY WHEN USING A COMPLEX MICROFEED PRODUCT IN NUTRITION

2021 ◽  
Vol 1 (1) ◽  
pp. 86-90
Author(s):  
L.A. Nikanova ◽  
Keyword(s):  
Green Algae ◽  
Blood Cells ◽  
White Blood Cells ◽  
Live Weight ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Blue Green Algae ◽  
Organic Iodine ◽  
Experimental Group

The object of research is the components of feed additives, namely dihydroquercetin, blue-green algae spirulina and organic iodine, in various combinations used in feeding piglets from the first days of feeding and before transferring them to fattening, at the age of 90 days. Three groups of piglets, one control and two experimental ones, were formed according to the principle of analogues. Piglets of the first experimental group received to the general diet, a feed additive MKBA-1, consisting of Spirulina – 1 mg, dihydroquercetin – 3 mg, organic iodine – 1,5 mg per 1,5 kg of live weight per day; piglets of the second experimental group to the main diet received a feed supplement ICBA-1, consisting of Spirulina – 2 mg and dihydroquercetin – 2 mg per 3 kg of live weight per day. The use of dihydroquercetin, blue-green algae spirulina and organic iodine in various combinations in feeding piglets contributed to an increase in the adaptive ability of the body, pathogenetic reactivity and correction of metabolic processes, which provided a higher realization of the genetic potential of pigs. The introduction of feed additives into the diet of pigs contributed to an increase in the bactericidal activity of blood serum in the first experimental group by 1%, in the second experimental group by 14,1%, compared with the control group. The number of white blood cells in piglets of the experimental groups was lower and red blood cells is higher compared to piglets in the control group, which was reflected in the hemoglobin content of blood, which was higher than piglets from the control group by 2% and 8,9%, respectively.

scholarly journals Influence of Omega-3 in Standardbred Horse: Haematological Parameters

2016 ◽  
Vol 16 (1) ◽  
pp. 145-154 ◽  
Author(s):  
Vincenzo Monteverde ◽  
Fulvio Congiu ◽  
Irena Vazzana ◽  
Salvatore Dara ◽  
Sergio Guttadauro ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Dietary Supplementation ◽  
Haematological Parameters ◽  
Control Group ◽  
Omega 3 ◽  
Blood Samples ◽  
Significant Difference ◽  
Athletic Horse ◽  
Experimental Group

Abstract The aim of this study was to investigate the effect of omega-3 supplementation on some haematological parameters like red blood cells (RBC), haemoglobin (Hgb), haematocrit (Hct), white blood cells (WBC), neutrophils (Neu) and lymphocytes (Lym) that may have a direct effect on the performance of athletic horses. Ten regularly trained Standardbred horses (6 geldings and 4 females, 4-5 years old, mean body weight 500 ± 25 kg) were used for the study. They were randomly divided into two groups. The experimental group received an omega-3 dietary supplementation every day for 30 days. The control group received no supplementation. Every 10 days, horses took part in a 1660 metre harness race and blood samples were collected from each horse: one in the morning before race (pre) and one after race (post). The application of one-way analysis of variance for repeated mesures (ANOVA) showed a statistically significant difference due to the race in the two different groups. The results obtained in the present study show a discordant effect of supplementation with omega-3 on RBC, HCT and Hgb, while omega-3 supplementation has been shown to have a better effect on WBC, Neu and Lym, but further studies should be performed to better evaluate the benefits of these on the performance of the athletic horse.

scholarly journals The effectiveness of the use of feed hydrolyzate in the poultry diet

2021 ◽  
Author(s):  
E.S. Eganjan ◽  
A.Kh. Shantyz ◽  
Yu.A. Lysenko ◽  
A.Yu. Shantyz ◽  
E.Yu. Marchenko
Keyword(s):  
Weight Gain ◽  
Blood Cells ◽  
Biochemical Composition ◽  
Egg Production ◽  
Laying Hens ◽  
White Blood Cells ◽  
Live Weight ◽  
Control Group ◽  
Positive Trend ◽  
Experimental Group

The authors of this article evaluated the effectiveness of the use of a feed additive based on soy protein hydrolysate in combination with iodine and selenium on laying hens of the Ross Hysex Brown cross. During the experiment, the following indicators were studied: safety, live weight gain, egg production, morpho-biochemical composition of blood. At the end of the experiment, the following data were obtained: the survival rate of the bird in the experimental group was 93,3 %, and in the control group the result was 86,6 %. The weight gain of laying hens in the experimental group increased by 11 %, the egg weight (average value) by 6,5 g (12,8 %), and in the control the increase was 2,7 g (5,2 %). In the experimental group, eggs per hen were obtained by 1,87% more than in the control group, in general, the experimental group showed an increase in the number of eggs during the experiment by 23,0 %. According to the results of the morphological and biochemical composition of the blood serum of birds, there are no significant negative changes. Red blood cells did not exceed intraspecific norms, but increased in the experimental group by 26,4 %. The amount of hemoglobin in the experimental group exceeded the control by 19,4 %. White blood cells in the experimental group were 16,9 % higher than this indicator in the control group. Biochemical analysis revealed a positive trend, which was expressed in an increase in the concentration of protein in the blood of chickens of the experimental group by 8,7 %, the level of calcium by 10,9 % and phosphorus by 31,3 % in birds of the experimental group exceeds the control.

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