Metaphylactic Antibiotic Treatment to Prevent the Transmission of Corynebacterium bovis to Immunocompromised Mouse Offspring

Current methods for eradicating Corynebacterium bovis, such as depopulation, embryo transfer, and cesarean rederivation followed by cross fostering, are expensive, complex, and time-consuming. We investigated a novel method to produce immunocompromised offspring free of C. bovis from infected NOD. Cg-PrkdcscidIl2rgtm1Wgl/SzJ (NSG) breeding pairs. Adult NSG mice were infected with C. bovis, paired, and randomly assigned to either a no-antibiotic control group (NAB, n = 8) or a group that received amoxicillin–clavulanic acid (0.375 mg/mL) in their drinking water for a mean duration of 7 wk (AB group, n = 7), spanning the time from pairing of breeders to weaning of litters. The AB group also underwent weekly cage changes for 3 wk after pairing to decrease intracage C. bovis contamination, whereas the NAB mice received bi-weekly cage changes. Antibiotics were withdrawn at the time of weaning. All litters (n = 7) in the AB group were culture- and qPCR-negative for C. bovis and remained negative for the duration of the study, whereas all litters in the NAB group (n = 6) remained C. bovis positive. A single adult from each breeding pair was sampled at weaning and at 5 and 10 wk after weaning to confirm the maintenance of (NAB) or to diagnose the reemergence (AB) of C. bovis infection. By the end of the study, C. bovis infection had returned in 3 of the 7 (43%) tested AB adults. Our data suggest that metaphylactic antibiotic use can decrease viable C. bovis organisms from adult breeder mice and protect offspring from infection. However, using antibiotics with frequent cage changing negatively affected breeding performance. Nevertheless, this technique can be used to produce C. bovis-free NSG offspring from infected adults and may be an option for salvaging infected immunocompromised strains of mice that are not easily replaced.

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Is perioperative antibiotic necessary in straightforward implant placement procedures?

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-19-00282 ◽

2020 ◽

Author(s):

Elçin Bedeloğlu ◽

Mustafa Yalçın ◽

Cenker Zeki Koyuncuoğlu

Keyword(s):

Clavulanic Acid ◽

Dental Implant ◽

Prophylactic Antibiotic ◽

Antibiotic Use ◽

Implant Failure ◽

Control Group ◽

Implant Placement ◽

Significant Difference ◽

Amoxicillin Clavulanic Acid ◽

Experimental Group

The purpose of this non-random retrospective cohort study was to evaluate the impact of prophylactic antibiotic on early outcomes including postoperative pain, swelling, bleeding and cyanosis in patients undergoing dental implant placement before prosthetic loading. Seventy-five patients (45 males, 30 females) whose dental implant placement were completed, included to the study. Patients used prophylactic antibiotics were defined as the experimental group and those who did not, were defined as the control group. The experimental group received 2 g amoxicillin + clavulanic acid 1 h preoperatively and 1 g amoxicillin + clavulanic acid twice a day for 5 days postoperatively while the control group had received no prophylactic antibiotic therapy perioperatively. Data on pain, swelling, bleeding, cyanosis, flap dehiscence, suppuration and implant failure were analyzed on postoperative days 2, 7, and 14 and week 12. No statistically significant difference was detected between the two groups with regard to pain and swelling on postoperative days 2, 7, and 14 and week 12 ( p >0.05), while the severity of pain and swelling were greater on day 2 compared to day 7 and 14 and week 12 in both groups ( p =0.001 and p <0.05, respectively). Similarly, no significant difference was found between the two groups with regard to postoperative bleeding and cyanosis. Although flap dehiscence was more severe on day 7 in the experimental group, no significant difference was found between the two groups with regard to the percentage of flap dehiscence assessed at other time points. Within limitations of the study, it has been demonstrated that antibiotic use has no effect on implant failure rates in dental implant surgery with a limited number of implants. We conclude that perioperative antibiotic use may not be required in straightforward implant placement procedures. Further randomized control clinical studies with higher numbers of patients and implants are needed to substantiate our findings.

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Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.1.23 ◽

2019 ◽

Vol 10 (01) ◽

Author(s):

Eman A. Al-Rekabi ◽

Dheyaa K. Alomer ◽

Rana Talib Al-Muswie ◽

Khalid G. Al-Fartosi

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Drinking Water ◽

Lipid Profile ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Male Rats ◽

Control Group ◽

Very Low Density Lipoprotein ◽

Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

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The use of acidifying agents Aquasafe and Veleguard when rearing of broiler chickens

Kormlenie sel'skohozjajstvennyh zhivotnyh i kormoproizvodstvo (Feeding of agricultural animals and feed production) ◽

10.33920/sel-05-2002-02 ◽

2020 ◽

pp. 16-27

Author(s):

L. Gamko ◽

T. Tarinskaya

Keyword(s):

Drinking Water ◽

Organic Acids ◽

Broiler Chickens ◽

Positive Impact ◽

The Body ◽

Metabolic Energy ◽

Control Group ◽

Meat Production ◽

Feed Conversion ◽

Broiler Meat

It is known that necessary to replace the components of organic acids, which are part of acidifiers when drinking water to poultry in order to prevent the adaptation of microbes in the gastrointestinal tract. In the poultry industry organic acids are widely used, which are used as acidifiers to preserve the properties of water consumed. The purpose of this work was to evaluate the efficiency of broiler meat production when using acidifying agents of drinking water Aquasafe and Veleguard. The experimental part of the work has been performed in JSC “Kurinoe Tsarstvo-Bryansk” broiler area “Roshcha” in the Pochepsky district. The object of research was the livestock of broiler chickens cross Cobb 500 at floor housing. The effect of water acidifiers on meat productivity has been studied. Groups of chickens have been formed on the principle of pairs-analogues. Broiler chickens of experimental groups have been separated from the main livestock by a grid in the corner of the room for 100 heads in each group. It has been found by a result of research to be optimal dose usage of acidifying agents water Aquasafe and Veleguard to drink to broiler chickens. The positive effect of these acidifiers on the digestion of crude protein, crude fat, crude fiber, contributing to the effective use of nitrogen, which led to an increase in the intensity of growth, young animals’ livability and improved feed conversion. Slaughter yield in the control group was 55,1 %, and in the experimental group 57,2 and 58,4 %, which was by 2,1 and 3,3 % higher with the same level of metabolic energy and nutrients. In chickens that consumed acidifi ers more intensively used nutrients feed for deposition in the body of the components of the carcass, which affected the production. A positive impact of acidifying agents Aquasafe and Veleguard on meat quality in broiler chickens has been found.

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Study on the Influence of Geometric Characteristics of Grain Membranes on Permeability Properties in Porous Sandstone

Membranes ◽

10.3390/membranes11080587 ◽

2021 ◽

Vol 11 (8) ◽

pp. 587

Author(s):

Run Shi ◽

Huaiguang Xiao ◽

Chengmeng Shao ◽

Mingzheng Huang ◽

Lei He

Keyword(s):

Fluid Flow ◽

Single Parameter ◽

Control Group ◽

Porous Rock ◽

Permeability Characteristics ◽

Natural Rock ◽

Porous Sandstone ◽

Novel Method ◽

Boltzmann Method ◽

Flow Laws

Studying the influence of grain characteristics on fluid flow in complex porous rock is one of the most important premises to reveal the permeability mechanism. Previous studies have mainly investigated the fluid flow laws in complex rock structures using an uncontrollable one single parameter of natural rock models or oversimplified control group models. In order to solve these problems, this paper proposes a novel method to reconstruct models that can independently control one single parameter of rock grain membranes based on mapping and reverse-mapping ideas. The lattice Boltzmann method is used to analyze the influence of grain parameters (grain radius, space, roundness, orientation, and model resolution) on the permeability characteristics (porosity, connectivity, permeability, flow path, and flow velocity). Results show that the grain radius and space have highly positive and negative correlations with permeability properties. The effect of grain roundness and resolution on permeability properties shows a strong regularity, while grain orientation on permeability properties shows strong randomness. This study is of great significance to reveal the fluid flow laws of natural rock structures.

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The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens

Animals ◽

10.3390/ani11020399 ◽

2021 ◽

Vol 11 (2) ◽

pp. 399

Author(s):

Magdalena Krauze ◽

Monika Cendrowska-Pinkosz ◽

Paulius Matuseviĉius ◽

Anna Stępniowska ◽

Paweł Jurczak ◽

...

Keyword(s):

Drinking Water ◽

Small Intestine ◽

Citric Acid ◽

Beneficial Effect ◽

Growth Performance ◽

Broiler Chickens ◽

Coliform Bacteria ◽

Control Group ◽

Cinnamon Oil ◽

Nonesterified Fatty Acids

It was postulated that a phytobiotic preparation containing cinnamon oil and citric acid added to drinking water for chickens in a suitable amount and for a suitable time would beneficially modify the microbiota composition and morphology of the small intestine, thereby improving immunity and growth performance without inducing metabolic disorders. The aim of the study was to establish the dosage and time of administration of such a phytobiotic that would have the most beneficial effect on the intestinal histology and microbiota, production results, and immune and metabolic status of broiler chickens. The experiment was carried out on 980 one-day-old male chickens until the age of 42 days. The chickens were assigned to seven experimental groups of 140 birds each (seven replications of 20 individuals each). The control group (G-C) did not receive the phytobiotic. Groups CT-0.05, CT-0.1, and CT-0.25 received the phytobiotic in their drinking water in the amount of 0.05, 0.1, and 0.2 mL/L, respectively, at days 1–42 of life (continuous application, CT). The birds in groups PT-0.05, PT-0.5, and PT-0.25 received the phytobiotic in the same amounts, but only at days 1–7, 15–21, and 29–35 of life (periodic application, PT). Selected antioxidant and biochemical parameters were determined in the blood of the chickens, as well as parameters of immune status and redox status. The morphology of the intestinal epithelium, composition of the microbiome, and production parameters of chickens receiving the phytobiotic in their drinking water were determined as well. The addition of a phytobiotic containing cinnamon oil and citric acid to the drinking water of broiler chickens at a suitable dosage and for a suitable time can beneficially modify the microbiome composition and morphometry of the small intestine (total number of fungi p < 0.001, total number of aerobic bacteria p < 0.001; and total number of coliform bacteria p < 0.001 was decreased) improving the immunity and growth performance of the chickens (there occurred a villi lengthening p = 0.002 and crypts deepening p = 0.003). Among the three tested dosages (0.05, 0.1, and 0.25 mL/L of water) of the preparation containing cinnamon oil, the dosage of 0.25 mL/L of water administered for 42 days proved to be most beneficial. Chickens receiving the phytobiotic in the amount of 0.25 mL/L had better growth performance, which was linked to the beneficial effect of the preparation on the microbiome of the small intestine, metabolism (the HDL level p = 0.017 was increased; and a decreased level of total cholesterol (TC) p = 0.018 and nonesterified fatty acids (NEFA) p = 0.007, LDL p = 0.041, as well as triacylglycerols (TAG) p = 0.014), and immune (the level of lysozyme p = 0.041 was increased, as well as the percentage of phagocytic cells p = 0.034, phagocytosis index p = 0.038, and Ig-A level p = 0.031) and antioxidant system (the level of LOOH p < 0.001, MDA p = 0.002, and the activity of Catalase (CAT) p < 0.001 were decreased, but the level of ferric reducing ability of plasma (FRAP) p = 0.029, glutathione p = 0.045 and vitamin C p = 0.021 were increased).

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Evaluation of Early Onset Sepsis, Complete Blood Count, and Antibiotic Use in Gastroschisis

American Journal of Perinatology ◽

10.1055/s-0037-1607420 ◽

2017 ◽

Vol 35 (04) ◽

pp. 385-389

Author(s):

Sadie Williams ◽

Matthew Leonard ◽

Eric Hall ◽

Jose Perez ◽

Jacqueline Wessel ◽

...

Keyword(s):

Early Onset ◽

Complete Blood Count ◽

Retrospective Chart Review ◽

Antibiotic Use ◽

Control Group ◽

Typically Develop ◽

Early Onset Sepsis ◽

Mature Neutrophil ◽

Reliable Marker ◽

Culture Positive

Objective Gastroschisis is a congenital defect in which the abdominal viscera herniate through the abdominal wall. In this population, antibiotics are often initiated immediately following delivery; however, this may be unnecessary as infections typically develop as a consequence of chronic issues in gastroschisis. The objective of this study was to evaluate the incidence of culture positive early onset sepsis, the reliability of the immature to mature neutrophil count (I:T) ratio as an infectious biomarker, and antibiotic use in infants with gastroschisis. Study Design This retrospective chart review analyzed clinical data from 103 infants with gastroschisis and 103 weight-matched controls that were evaluated for early onset infection. Results Compared with the control group, there was a significantly increased percentage of infants with an I:T ratio > 0.2 in the gastroschisis group (43% vs. 12%, p < 0.001) and an increased percentage of infants exposed to greater than 5 days of antibiotics regardless of their I:T ratio (75% vs. 6%, p < 0.001). There were no episodes of culture positive early onset sepsis in either group. Conclusion Our results indicate that I:T ratio is not a reliable marker of infection in gastroschisis, and suggest that empiric septic evaluation and antibiotic use, immediately following delivery in gastroschisis infants, may be unnecessary.

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Supplementary feeding of Merino wethers grazing weed-free stubble pastures

Australian Journal of Experimental Agriculture ◽

10.1071/ea9810272 ◽

1981 ◽

Vol 21 (110) ◽

pp. 272 ◽

Cited By ~ 3

Author(s):

LG Butler

Keyword(s):

Drinking Water ◽

Field Trial ◽

Compensatory Growth ◽

Supplementary Feeding ◽

Control Group ◽

Apparent Digestibility ◽

Wool Production ◽

Wool Growth ◽

Wheat Stubble

A field trial was conducted in the Wimmera region of Victoria to investigate the effect of several supplements on 4-year-old Merino wethers grazing a weed-free wheat stubble for 3 months, The four treatments were: a control (no supplement), urea/molasses block, 100 g/head day-1 of lupin grain, and 2 g urea plus 0.5 g Na2SO4 l-1 in the drinking water. Twenty sheep per treatment were used, and liveweight change and wool growth were measured. Although all groups lost weight, the lupin group lost only 0.5 kg per head over the 3 months of the trial, and was 3.8 kg heavier (P <0.01) than the control group, while the urea/molasses group was 2.2 kg heavier (P <0.01) than the control group. There was no effect of the urea plus sulfur supplement in the drinking water on liveweight or wool production. The urea/molasses block did not affect wool production but lupin grain stimulated wool production by 0.08 kg (P <0.05). The sheep were weighed and shorn four and a half months after the trial (August), during which time all groups grazed together on green feed. The experimental liveweight differences had disappeared, apparently due to compensatory growth. In addition, a digestibility trial was conducted in an attempt to explain some of the field results. The treatments were designed to simulate those used in the field. Lupin grain stimulated intake by 129 g day-1 (P <0.05) but had no effect on apparent digestibility of straw. The treatment simulating the urea/ molasses block (urea plus sulfur plus sucrose in the drinking water) stimulated intake by 159 g day-l (P <0.05) and tended to increase digestibility (P > 0.05). It was concluded that supplementary feeding of Merino wethers grazing weed-free stubble is unlikely to be an economic farm practice.

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Effect of the Feed Additive Clinoptilolite (ZeoFeed) on Nutrient Metabolism and Production Performance of Laying Hens

Acta Veterinaria Brno ◽

10.2754/avb201079s9s029 ◽

2010 ◽

Vol 79 (9) ◽

pp. S29-S34 ◽

Cited By ~ 6

Author(s):

Miroslav Macháček ◽

Vladimír Večerek ◽

Nora Mas ◽

Pavel Suchý ◽

Eva Straková ◽

...

Keyword(s):

Drinking Water ◽

Laying Hens ◽

Laying Hen ◽

Production Performance ◽

Feed Consumption ◽

Control Group ◽

Egg Weight ◽

Indicator Method ◽

Balance Tests ◽

Gross Energy

The aim of this study was to assess the effects of two levels of clinoptilolite administered in feed (2% and 4%) on some selected performance indicators, metabolic utilization of basic nutrients and the health status of laying hens. The selected 24 Bovans Goldline hybrid laying hens were divided into three equal groups, two experimental groups (E1 and E2) and one control group (C). The laying hens were housed individually in cages with an automatic supply of drinking water, manual feeding, in a setting with controlled light and temperature regimens. Hens from individual groups were all fed a complete feed mix of the same composition and the only difference was in clinoptilolite supplementation: feed mixes for E1 and E2 groups contained 2% and 4% of clinoptilolite (commercial additive ZeoFeed) respectively, replacing the same amounts of wheat. The hens received feed mixes and drinking water ad libitum. During this 28-day experiment, feed consumption and the number and weight of eggs laid were monitored individually for each hen. At the end of the experiment, the balance test using the indicator method (Cr2O3) was performed in all eight hens in each of the groups. The results of balance tests were then used to calculate the metabolic utilization of selected nutrients (nitrogen, fat, ash, nitrogen-free extracts, starch, gross energy, Ca, P). After the balance tests, blood samples for haematological and biochemical examinations were collected via puncture of the vena basilica. The addition of 2% clinoptilolite to feed mix resulted in a highly significant (P ⪬ 0.01) increase in mean egg weight to 64.69 g, but the addition of 4% clinoptilolite in group E2 resulted in a highly significant (P ⪬ 0.01) decrease in mean egg weight to 62.20 g compared to the control (63.73 g). Moreover, daily feed mix consumption in group E1 decreased to 114 g per one laying hen/day compared to the controls (118 g per one laying hen/day). In group E2 (4% clinoptilolite), daily consumption of feed mix increased compared with the controls to 124 g. The 2% clinoptilolite supplementation of E1 group feed slightly increased metabolic utilization of fat, nitrogen-free extracts, starch and gross energy compared to group C. Results of haematological test of the hens’ blood showed significant changes in haemoglobin, whose values in groups C and E1 were significantly higher (P ⪬ 0.05) than in group E2. Differences in the values of the biochemical indicators monitored (total protein, glucose, cholesterol, triacylglycerols, calcium, phosphorus, magnesium and the AST enzyme) between group C and experimental groups E1 and E2 were not significant and remained within the range of reference values.

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Mineralocorticoid Receptor Activation by Aldosterone or Corticosterone Induces Hypertension, Myocardial Infarction and Proteinuria

Hypertension ◽

10.1161/hyp.36.suppl_1.723-a ◽

2000 ◽

Vol 36 (suppl_1) ◽

pp. 723-723

Author(s):

Qing-Feng Tao ◽

Diego Martinez vasquez ◽

Ricardo Rocha ◽

Gordon H Williams ◽

Gail K Adler

Keyword(s):

Myocardial Infarction ◽

Drinking Water ◽

Mineralocorticoid Receptor ◽

Critical Role ◽

Weight Ratio ◽

Myocardial Necrosis ◽

Receptor Activation ◽

Control Group ◽

Sprague Dawley ◽

Sprague Dawley Rats

P165 Aldosterone through its interaction with the mineralocorticoid receptor (MR) plays a critical role in the development of hypertension and cardiovascular injury (CVI). Normally, MR is protected by 11β-hydroxysteroid dehydrogenase (11β-HSD) which inactivates glucocorticoids preventing their binding to MR. We hypothesis that if activation of MR by either aldosterone or glucocorticoids induces hypertension and CVI, then the inhibition of 11β-HSD with glycyrrhizin (GA), a natural inhibitor of 11β-HSD, should induce damage similar to that observed with aldosterone. Sprague-Dawley rats were uninephrectomized, and treated for 4 weeks with 1% NaCl (in drinking water) for the control group, 1% NaCl + aldosterone infusion (0.75 μg/h), or 1% NaCl + GA (3.5 g/l in drinking water). After 4 weeks, aldosterone and GA caused significant increases in blood pressure compared to control rats ([mean ± SEM] 211± 9, 205 ± 12, 120 ± 9 mmHg, respectively, p<0.001). Both aldosterone- and GA-treated rats had a significant increase in proteinuria (152.2 ± 8.7 and 107.7 ± 19.5 mg/d, respectively) versus controls (51.2 ± 9.5 mg/d). There was a significant increase (p<0.001) in heart to body weight ratio in the rats treated with aldosterone or GA compared with control (3.92 ± 0.10, 3.98 ± 0.88, and 3.24 ± 0.92 mg/g, respectively). Hearts of GA and aldosterone treated rats showed similar histological changes consisting of biventricular myocardial necrosis and fibrinoid necrosis of small coronary arteries and arterioles. These data suggests that in rodents activation of MR by either aldosterone or corticosterone leads to severe hypertension, vascular injury, proteinuria and myocardial infarction. Thus, 11β-HSD plays an important role in protecting the organism from injury.

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REACTION OF CD68-POSITIVE RAT LIVER AND SPLEEN CELLS ON SILICON INTAKE WITH DRINKING WATER

Acta medica Eurasica ◽

10.47026/2413-4864-2021-2-34-43 ◽

2021 ◽

pp. 34-43

Author(s):

Evgeniia A. Grigoreva ◽

Valentina S. Gordova ◽

Valentina E. Sergeeva ◽

Alina T. Smorodchenko

Keyword(s):

Drinking Water ◽

Cytoplasmic Membrane ◽

Unit Area ◽

Morphological Changes ◽

Morphological Characteristics ◽

Control Group ◽

Silicon Compound ◽

Average Cell ◽

Laboratory Rats ◽

Experimental Group

The article presents data on the long-term effect (nine months) of a silicon compound supplied with drinking water – nonahydrate sodium metasilicate (10 mg/l in terms of silicon), on CD68-positive macrophages in the liver and spleen of laboratory rats. Changes in the morphological characteristics of this cell population were found. There was a decrease in the average cell area (in the liver of the control group of rats, the average macrophage area was 179.23±5.94 microns2, and in the group receiving silicon with drinking water – 117.04±3.35 microns2; in the spleen-136.02±3.93 microns2 and 103.44±2.8 microns2, respectively). Macrophages in the liver preparations of the experimental group of rats had a fewer processes and a darker cytoplasmic membrane. The number of macrophages in the liver per unit area was comparable, for the control group of rats it was 18.78±1.24, and for the rats that received with water with the addition of silicon – 19.41±0.75 cells. CD68+ macrophages of the red splenic pulp in laboratory rats that received silicon also underwent the following morphological changes: they were located in a denser way and had fewer processes, while the number of macrophages per unit area was 73.7±2.3 for the control group, 91.6±5.0-for the experimental group, respectively. The distance between them did not change. There was a change in the intensity of CD68 expression on the surface of the cytoplasmic membrane and in the cytoplasm of liver and spleen macrophages. These changes can be interpreted as the adaptive ability of liver and spleen macrophages to silicon introduced with drinking water. Given the heterogeneity of the macrophage population in the liver and spleen, further studies using markers for different subpopulations of macrophages are needed to clarify their role in the response of tissues to silicon supplied with drinking water.

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