scholarly journals Efficacy of solid dispersion of fenbendazole against gastrointestinal strongylatosis of young cattle

2021 ◽  
Vol 15 (1) ◽  
pp. 92-97
Author(s):  
A. I. Varlamova ◽  
I. A. Arkhipov ◽  
K. M. Sadov ◽  
S. S. Khalikov ◽  
M. V. Arisov ◽  
...  
Keyword(s):  
Solid Dispersion ◽  
Mechanical Mixture ◽  
Control Group ◽  
Control Test ◽  
Mechanochemical Processing ◽  
Basic Drug ◽  
Group V ◽  
Anthelmintic Efficacy ◽  
Young Cattle ◽  
Flotation Method

The purpose of the research is to study the efficacy of solid dispersion of fenbendazole (SDF) against nematodoses of young cattle.Materials and methods. The study of SDF, obtained by mechanochemical processing of fenbendazole substance with polyvinylpyrrolidone (PVP) polymer, was carried out on 126 young cattle naturally infected with nematodiroses and other gastrointestinal strongylatoses. The animals were divided into 6 experimental groups of 10–11 animals each and SDF was administered once orally at doses of 2.0; 3.0 and 4.0 mg/kg of active substance (a.s.) (I-III groups) in comparison with the mechanical mixture of fenbendazole (FBZ) and PVP in a ratio of 1:9 (IV group) and substance FBZ at a dose of 3.0 mg/kg (group V) against each helminthosis. The control group of animals did not receive the drugs. Anthelmintic efficacy was evaluated in the "control test" based on the data of ovoscopic examination of feces of young cattle by flotation method before and 17 days after drugs administration.Results and discussion. SDF with PVP at a dose of 2.0; 3.0 and 4.0 mg/kg of a.s. in the form of a 10% powder showed respectively 88.4; 97.3 and 100% efficiency at nematodiroses and 89.2; 98.4 and 99.5 % activity against other types of gastrointestinal strongylates upon obtaining 32.3 and 32.4 % effect of the mechanical mixture of FBZ with PVP and 29.7 and 27.4% efficiency of the base preparation at a dose of 3.0 mg/kg. SDF showed 88.4; 97.3 and 100% of efficacy at doses of 2.0; 3.0 and 4.0 mg/kg of a.s., respectively, against nematodiroses and 89.2; 98.4 and 99.5% against other gastrointestinal strongylatoses in the form of 10% powder. It should be noted that mechanical mixture of PBZ with PVP showed 32.3 and 32.4% efficacy and the efficacy of the basic drug was 29.7 and 27.4% at a dose of 3.0 mg/kg against each helminthosis.

scholarly journals The Influence of Mechanochemical Technology on Anthelmintic Efficacy of Supramolecular Complexes of Fenbendazole with Licorice Extract

2020 ◽  
Vol 14 (1) ◽  
pp. 70-74
Author(s):  
Ivan A. Arkhipov ◽  
Anastasia I. Varlamova ◽  
Salavat S. Khalikov ◽  
Konstantin M. Sadov ◽  
Alexander V. Dushkin
Keyword(s):  
Oral Administration ◽  
Supramolecular Complexes ◽  
Control Test ◽  
Basic Drug ◽  
Test Type ◽  
Anthelmintic Efficacy ◽  
Single Oral Administration ◽  
Licorice Extract

The purpose of the research is studying the effect of mechanochemical technology on anthelmintic efficacy of supramolecular complexes of fenbendazole with licorice extract. Materials and methods. Tests of the supramolecular complexes of fenbendazole (SMCF) with licorice extract (LE) were carried out on 127 heads of young sheep spontaneously infected by Nematodirus sp. and other types of gastrointestinal strongylates. For each helminthosis, animals of different groups of 8–11 animals each were given single oral administration of SMCF with LE in a dose of 2.0 mg/kg in AD in the form of 10 and 17 % powders and 2.4 and 4.5% suspensions in comparison with the basic drug – fenbendazole (FBZ) in a dose of 2.0 mg/kg. SMCF with LE was developed according to mechanochemical technology for 2 hours. The baseline was a group of sheep that did not receive the drug. The anthelmintic efficacy of the preparations was taken into account according to the results of coproovoscopic studies of sheep by flotation before and 16 days after the preparations were used. Accounting for the effectiveness of drugs was carried out according to the "control test" type. Results and discussion. SMСF with LE in a dose of 2.0 mg/kg by AD in the form of 10 and 17% powders and 2.4 and 4.5% suspensions showed respectively 89.2; 83.12; 82.09 and 83.59% effectiveness against Nematodirus spp. and 88.73; 67.3; 83.87 and 86.38% activity against other types of Strongylata when receiving 22.7 and 21.9% effect of the base drug – FBZ.

scholarly journals Efficacy of the supramolecular complex of Fenbendazole against Nematodes in a commission and Production test

2020 ◽  
Vol 14 (2) ◽  
pp. 93-97
Author(s):  
A. I. Varlamova ◽  
I. A. Arkhipov ◽  
K. M. Sadov ◽  
S. S. Khalikov
Keyword(s):  
Drug Efficacy ◽  
Supramolecular Complex ◽  
Control Group ◽  
Production Test ◽  
Not Given ◽  
Control Test ◽  
Flotation Method ◽  
Combined Infection ◽  
Major Drug ◽  
Stock Feed

The purpose of the research is determining the efficacy of the supramolecular complex of fenbendazole (SMCF) based on the nano-sized supramolecular delivery system with polyvinylpyrrolidone (PVP) against nematodes in sheep in a commission and production test. Materials and methods. A commission test was carried out at Izmailov LLC in the Krasnoarmeysky District of the Samara Region in August 2019. The SMCF at a dose of 2.0 mg/kg by the active substance was given to sheep (30 animals) of different age. The substance of fenbendazole was used as the major drug at a dose of 5.0 mg/kg in 20 sheep. A group of 20 sheep who were not given the drug was a control group. The drug efficacy was recorded by the flotation method according to results of the coproovoscopic studies of sheep before and 16 days after the drugs were administered. The drug efficacy was calculated by a “control test”. The production test of the SMCF in gastrointestinal strongylatosis of sheep was carried out on 120 wether hoggs at the same farm. The SMCF was prescribed to sheep once at a dose of 2.0 mg/kg by the AS in a mixture with 0.3 kg of oatmeal stock feed (per animal) given for the whole group. The efficacy of the SMCF was recorded according to the results of the coproovoscopic studies before and 15 days after deworming. Results and discussion. In the commission test of 70 sheep with combined infection, the SMCF with PVP at doses of 3.0 and 2.0 mg/kg by the AS showed 98.7% effectiveness against Nematodirus infection and 99.2% activity against other types of gastrointestinal Strongylata. In the production test, the SMCF at a dose of 2.0 mg/kg by the AS showed a 99.1 % effect against nematodirosis and 98.8% against other gastrointestinal strongylatoses. 

The Quantitative Ultrastructure of the Heart Muscle of Japanese Quail by Hypergravitation, Hypodynamy and Combination

1990 ◽  
Vol 48 (3) ◽  
pp. 188-189
Author(s):  
Anton Bózner ◽  
Mikuláš Gažo ◽  
Jozef Dostál
Keyword(s):  
Japanese Quail ◽  
Heart Muscle ◽  
Relative Size ◽  
Combination Group ◽  
Control Group ◽  
Group V ◽  
Group Iii ◽  
Space Flights ◽  
Group I ◽  
Quantitative Ultrastructure

It is anticipated that Japanese quail /Coturnix coturnix japonica/ will provide animal proteins in long term space flights. Consequently this species of birds is of research interest of international space program INTERCOSMOS. In the year 1987 we reported on an experiment /2/ in which the effect of chronic acceleration of 2 G hypergravitation, the hypodynamy and the simultaneous effect of chronic acceleration and the location in the centre of the turntable of the centrifuge on the protein fractions in skeletal muscles was studied. The ultrastructure of the heart muscle was now in this experiments examined as well.Japanese quail cockerels, aged 48 days were exposed to 2 G hypergravitation /group IV/ in a 6,4 m diameter centrifuge, to hypodynamy /group III/ and their combination /group V/, respectively for 6 days / Fig.1/. The hypodynamy in group III was achieved by suspending the birds in jackets without contact the floor. The group II was located in the centre ofthe turntable of the centrifuge. The control group I. was kept under normal conditions. The quantitative ultrastructure of myocard was evaluated by the methods of Weibel/3/ - this enables to determine the number, relative size and volume of mitochondria volume of single mitochondria, defficiency of mitochondrial cristae and volume of myofibrils.

The effects of desferrioxamine on cisplatininduced lipid peroxidation and the activities of antioxidant enzymes in rat kidneys

2004 ◽  
Vol 23 (1) ◽  
pp. 29-34 ◽  
Author(s):  
G Kadikoylu ◽  
Z Bolaman ◽  
S Demir ◽  
M Balkaya ◽  
N Akalin ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Vitamin C ◽  
Isotonic Saline ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Group V ◽  
Super Oxide Dismutase ◽  
Cervical Dislocation ◽  
Rat Kidneys

Cisplatin-induced nephrotoxicity is associated with an increase in lipid peroxidation and oxygen free radicals in rat kidneys. In this study, the effects of desferrioxamine were compared to vitamin C and E on cisplatin-induced lipid peroxidation and antioxidant enzyme activities in rat kidneys. Rats were divided into five groups, with 15 Wistar rats in each group. In the control group, rats received 1 mL/100 g isotonic saline solution intraperitoneally (i.p.). In Group II, 10 mg/kg cisplatin i.p. was injected to rats. Thirty minutes before the same dosage of cisplatin administration, 100 mg/kg i.p. vitamin C or E was given to rats in groups III and IV, respectively. Rats in Group V received 250 mg/kg desferrioxamine i.p., before the same dose of cisplatin administration. All rats were killed by cervical dislocation after 72 hours. The kidneys were immediately removed and washed in cold saline. Spectrophotometric method was used for all analyses. While catalase, glutathione reductase (GR), and super oxide dismutase (SOD) levels were found to be significantly decreased (P B < 0.001), malondialdehyde (MDA) (P < 0.05) and hydrogen peroxide (H2O2) (P < 0.001) levels were significantly increased in the cisplatin group when compared to the controls. MDA levels were decreased by desferrioxamine (P < 0.005) as well as vitamin C and E (P < 0.05 and P < 0.001, respectively). These three compounds induced a significant increase in SOD levels (P B < 0.05), but only in the vitamin C group, were SOD levels not significantly different than the levels of the controls (P > 0.05). In the desferrioxamine (P < 0.05), vitamin C and E groups (P < 0.001 for both), the cisplatin elevated H2O2 levels were decreased. None of these drugs had any effect on GR and catalase levels (P > 0.05). Desferrioxamine is useful to prevent cisplatin-induced lipid peroxidation, however, vitamin C and E are more effective on antioxidant enzymes than desferrioxamine.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

The influence of highly dispersed preparations on metabolism and productivity of young cattle

2020 ◽  
pp. 22-33
Author(s):  
E. A. Sizova ◽  
A. M. Makaeva
Keyword(s):  
Trace Elements ◽  
High Surface ◽  
Live Weight ◽  
Animal Husbandry ◽  
Control Group ◽  
Dispersed Particles ◽  
Highly Dispersed ◽  
Young Cattle ◽  
Experimental Group ◽  
Highly Dispersed Particles

Preparations of highly dispersed particles of trace elements are increasingly used in animal husbandry. This is determined by their extraordinary biological properties such as the ability to penetrate into tissues and organs, a high surface area, and so on. One of the promising directions for using highly dispersed particles both in the post-embryonic and embryonic periods of animal development is the use of trace elements as sources. This is determined by the relatively lower toxicity, higher bioavailability of elements from preparations of highly dispersed particles, which reduces the load on the environment and allows you to produce products enriched with minerals. The purpose of the research was to study the effect of highly dispersed drugs on rumen digestion, the composition of the rumen microbiome, metabolism and productivity of young cattle. A comprehensive assessment of the use of highly dispersed SiO2 and FeCo preparations in cattle nutrition has been provided in the paper. The digestibility of feed, metabolism, and productivity of young cattle when feeding highly dispersed particles have been studied. An unusual fact of increasing bacterial biomass when using highly dispersed particles of silicon dioxide in animal feeding has been described. A method for increasing the digestibility of feed components by ruminants through the use of highly dispersed FeCo alloy particles has been proposed. As follows from the data obtained the use of highly dispersed particles allowed to increase the live weight of experimental young animals to 413 kg in the 1st experimental group and 416 kg in the 2nd experimental group, which was by 11 (P ≤ 0,01) and 14 kg (P ≤ 0,01) higher than the control indicator. As follows from the analysis of the data obtained, the profitability of rearing young animals in the 1st and 2nd experimental groups was by 2,4 and 2,2 % higher than the same indicator calculated for the control group.

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

scholarly journals Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

2012 ◽  
Vol 27 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Conceição Aparecida Dornelas ◽  
Francisco Vagnaldo Fechine-Jamacaru ◽  
Irineu Lima Albuquerque ◽  
Hemerson Iury Ferreira Magalhães ◽  
Adjair Jairo Silva de Souza ◽  
...  
Keyword(s):  
Group Iv ◽  
The Other ◽  
Control Group ◽  
Group Viii ◽  
Group Vi ◽  
Group V ◽  
Induction Group ◽  
Rat Bladder ◽  
Bladder Carcinogenesis ◽  
Group I

PURPOSE: To determine the effects of green propolis extracted in L-lysine (WSDP) and of L- lysine for 40 weeks on induced rat bladder carcinogenesis. METHODS: The animals (groups I, II, III, IV, V and VI) received BBN during 14 weeks. Group I was treated with propolis 30 days prior received BBN, and then these animals were treated daily with propolis; Groups II and III was treated with subcutaneous and oral propolis (respectively) concurrently with BBN. The animals of Group IV were treated L-lysine; Group V received water subcutaneous; and Group VI received only to BBN. Among the animals not submitted to carcinogenesis induction, Group VII received propolis, Group VIII received L-lysine and Group IX received water. RESULTS: The carcinoma incidence in Group I was lower than that of control (Group VI). The carcinoma multiplicity in Group IV was greater than in Group VI. All animals treated with L-lysine developed carcinomas, and they were also more invasive in Group IV than in controls. On the other hand, Group VIII showed no bladder lesions. CONCLUSION: The WSDP is chemopreventive against rat bladder carcinogenesis, if administered 30 days prior to BBN , and that L-lysine causes promotion of bladder carcinogenesis.

scholarly journals Comparative Evaluation between Sulfasalazine Alone and in Combination with Herbal Medicine on DSS-Induced Ulcerative Colitis Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Mi-Rae Shin ◽  
Kyeong Jo Kim ◽  
Soo Hyun Kim ◽  
Su Ji Kim ◽  
Bu-Il Seo ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Comparative Evaluation ◽  
Control Group ◽  
Inflammatory Infiltration ◽  
Group V ◽  
Group Iii ◽  
Protein Levels ◽  
Group I ◽  
Apoptotic Protein ◽  
Mitogen Activated Protein

The present study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and sulfasalazine in combination with herbal medicine on dextran sodium sulfate- (DSS-) induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n=9): Group I (normal group), Group II (DSS control group), Group III (DSS + sulfasalazine (30 mg/kg)), Group IV (DSS + sulfasalazine (60 mg/kg)), and Group V (DSS + sulfasalazine (30 mg/kg) + Cinnamomi Cortex and Bupleuri Radix mixture (30 mg/kg) (SCB)). Colonic pathological changes were analyzed using hematoxyline/eosin staining. The antioxidant, inflammatory, and apoptotic protein levels were determined using western blotting. SCB supplementation, as well as sulfasalazine, suppressed colonic length and mucosal inflammatory infiltration. In addition, SCB treatment significantly reduced the expression of proinflammatory signaling molecules through suppression of both mitogen-activated protein kinases (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways and prevented the apoptosis of the colon. Moreover, SCB administration significantly led to the upregulation of antioxidant enzymes including SOD and catalase. Taken together, SCB treatment might offer a better treatment for human UC than sulfasalazine alone or may be useful as an alternative therapeutic strategy against UC, without any evidence of side effects.

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