scholarly journals Preclinical aphrodisiac investigation of ethanol extract of Flueggea leucopyrus Willd. leaves

2018 ◽  
Vol 7 (3) ◽  
pp. 319-324
Author(s):  
Sharad Vasantrao Usnale ◽  
◽  
Kailash Radhesham Biyani ◽  
Keyword(s):  
Sperm Motility ◽  
Morphological Study ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Reproductive Organs ◽  
Albino Rats ◽  
Experimental Animals ◽  
Morphological Studies ◽  
Intromission Latency

Sexual dysfunction is a widely found disorder worldwide now a day because of busy lifestyle and stress. Management of disease is somewhat difficult because of cost and shortage of effective treatment. In the present paper authors have investigated effect of ethanolic extract of Flueggea leucopyrus Willd. leaves as aphrodisiac potential on albino rats. The dried leaves of plant were extracted by cold maceration method using ethanol. It was investigated for in vivo aphrodisiac activity. Plant extract was administered at doses 200 and 400 mg/kg body weight for 45 days. The effect of extract on general mating behavior, potency test, morphological studies, organ weight and sperm motility were tested. The results were compared with standard reference sildenafil citrate. The acute toxicity of the drug extract was also checked. On oral administration of ethanol extract 200 and 400 mg/kg doses on 15th, 30th and 45th day of treatment in all treated groups significantly increased intromission latency, mounting frequency, ejaculatory latency and significantly decreased intromission latency, mounting latency, inter intromission interval and post ejaculatory interval. Test for potency showed significant increase in long flips, quick flips and erections. In all experimental animals morphological study showed significant increase in main and accessory reproductive organs weight and sperm motility. As compared to the control the prolonged treatments for all treated groups were highly effective. This showed that aphrodisiac activity has been shown by ethanol extract but it is less than the standard. The present study showed that ethanol extract of Flueggea leucopyrus Willd. leaf increase sexual behavior in rats. Thus it supports the claim for this drug as an aphrodisiac

Evaluation of Preclinical Aphrodisiac Activity of Flueggea leucopyrus Willd. Leaves

2020 ◽  
Vol 16 (3) ◽  
pp. 335-341
Author(s):  
Usnale S. Vasantrao ◽  
Kailash R. Biyani
Keyword(s):  
Morphological Study ◽  
Sperm Count ◽  
Water Extract ◽  
Reproductive Organs ◽  
Phytochemical Analysis ◽  
Experimental Animals ◽  
Intromission Latency ◽  
Solvent Control ◽  
Intromission Frequency

Background: Flueggea leucopyrus Willd. leaf has been used since ancient time for its sex enhancing activity by traditional physicians. So, the work was chosen to authenticate the knowledge of pharmaceutical significance of the plant. Methods: The dried leaves of Flueggea leucopyrus were extracted by cold maceration method using water. Aqueous extract of Flueggea leucopyrus leaf was screened for phytochemicals and in vivo aphrodisiac activity using mating behavior, potency and morphological tests. Results: Qualitative phytochemical analysis of leaves of Flueggea leucopyrus showed the presence of alkaloids, terpenoids, unsaturated sterols, glycosides, saponins, phenolics, flavonoids, tannins, carbohydrates and protein. Results revealed that all the treated groups on the 15th, 30th and 45th day of treatment showed significant increase in Intromission Frequency, Mounting Frequency and Ejaculatory Latency significant decrease in Intromission Latency, Mounting Latency, Inter Intromission Interval and Post Ejaculatory Interval. In Test for Potency significant increase in Erections, Long Flips and Quick Flips were observed. Morphological study showed significant increase in reproductive organs weight and Sperm Count in all the experimental animals. Conclusion: The prolonged treatments for all the treated groups were highly effective to increase the sexual libidity, as compared to the solvent control. This indicates that aphrodisiac activity has been shown by water extract but it is less than the standard used for study.

scholarly journals Hepatoprotective effects of ethanol extract of Caesalpiniabonduc against carbon tetrachloride induced hepatotoxicity in Albino Rats

2016 ◽  
Vol 20 (2) ◽  
pp. 396-401
Author(s):  
A.E. Ubhenin ◽  
I Igbe ◽  
F.A. Adamude ◽  
A Falodun
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Serum Marker ◽  
Albino Rats ◽  
Different Doses

The present study was carried out to evaluate the Acute toxicity, hepatoprotective and in-vivo antioxidant activities of ethanolic extract of Caesalpinia bonduc leaf on carbon tetrachloride (CCl4) induced liver damage using Swiss albino rats . The ethanolic extract of the plant of C. bonduc were suspended in 5 % tragacanth and then administered orally at doses of 250 and 500 mg/kg body weight for fourteen days before intraperitoneally injection of Carbon tetrachloride (CCl4) at dose of 2 mL/kg body weight. The plant extracts at 250 and 500mg/kg b.wt showed a remarkable hepatoprotective and invivo antioxidant activities against carbon tetrachloride CCl4 – induced hepatotoxity judged from the serum marker enzymes .The CCl4 induced significant increase in aspartate amino transferase( AST), alanine amino transferase (ALT), alkaline phophatase (ALP), total bilirubin, and malondyaldehyde (MDA) with a reduction of total protein, catalase, and glutathione peroxidase . Treatment of rats with different doses of plant extract (250 and 500 mg/kg b.wt.) significantly (P< 0.001) altered serum maker enzymes and antioxidant levels to near normal levels. The study suggests that C. Bonduc specifically chloroform and ethyl acetate fraction may be good sources of natural antioxidant and hepatoprotective substance.Keywords: Ceasalpiniabonduc, hepatoprotective, intraperitoneally , antioxidant

scholarly journals Hepatoprotective potential of Indigofera tirunelvelica Sanjappa: in vitro and in vivo studies on CCl4 induced wistar albino rats

2020 ◽  
Vol 11 (4) ◽  
pp. 6404-6410
Author(s):  
Subburayalu S ◽  
Asha KRT ◽  
Deepa Somanath ◽  
Palavesam A
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Albino Rats ◽  
Whole Plant ◽  
Wistar Albino Rats

The hepatoprotective efficiency of Indigofera tirunelvelica Sanjappa whole plant against CCl4 induced hepatotoxicity was examined. Rat hepatocyte monolayer culture and wistar albino rats were exercised as in vitro and in vivo screening models of protective agent for liver. In in vitro analyses, the whole plant ethanolic extract of Indigofera tirunelvelica Sanjappa were inspected. Silymarin was chosen as a standard treatement drug. In vitro, free radical scavenging property was also evaluated. In animal studies, hepatotoxicity was produced in Wistar albino rats by dispensing CCl4. The degree of hepatotoxicity was examined by determining the ranges of serum enzyme. The antioxidant parameters such as superoxide dismutase, catalase, reduced glutathione, and malondialdehyde of the hepatocytes were also evaluated. In in vitro studies, ethanol extract of I. tirunelvelica whole plant was identified to be the most active than other assessed extracts. Besides, whole plant ethanol extract of I. tirunelvelica was noticed to be rich in phenolic and flavonoids. It exhibited expressive free radical scavenging property versus diphenylpicryl hydrazyl (DPPH) and superoxide ion radicals. In the animals studies, whole plant ethanolic extract of I. tirunelvelica at a ranges of doses (100, 200 and 400 mg/kg body weight) revealed considerable amount of protection against CCl4 induced hepatotoxicity as evident by the protection of CCl4 induced changes biochemical parameters. The results of the present study suggested that the significant hepatoprotective property of whole plant ethanol extract of I. tirunelvelica against CCl4 induced hepatotoxicity and intimates its use as a potential medicinal drug for liver diseases.

scholarly journals Subacute Toxicity Effect of Psidium guajava Leaves Ethanolic Extract on Gaster in Wistar Rat

2018 ◽  
Vol 16 (1) ◽  
pp. 61
Author(s):  
Esti Dyah Utami ◽  
Hanif Nasiatul Baroroh ◽  
Nuryanti Nuryanti
Keyword(s):  
Toxic Effect ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Psidium Guajava ◽  
Albino Rats ◽  
Respiratory Disorder ◽  
Subchronic Toxicity ◽  
Toxicity Effect ◽  
Test Materials

Psidium guajava leaves have been proven be antiallergic. From the results of previous research, the ethanol extract of leaves is included in the class materials "Practically Non-Toxic." However, repeated administration may cause toxic effects on body organs, one of which was gaster. Subchronic toxicity had been carried out on 20 male Wistar rats and 20 female wistar albino rats. The test materials were administered orally, every day for 28 days. There are 3 (three) doses of test materials which were 250 mg, 500 mg, and 750 mg/kg BW. Observation of the general toxic effect was performed everyday, then organ sampling was performed on day 29 to examined gaster histology and score using Barthel-Manja methods. The result showed that administration of Psidium guajava leaves extract for 28 days caused physical and behavioral changes, caused soft and mucous feces, and also respiratory disorder. But the extract at dose 250 mg, 500 mg and 750 mg/kg BW did not caused changes on gaster histology profile and score. In conclusion, the extract did not have subchronic toxicity effect on gaster rat, but might caused general toxic effect in the higher dose.

scholarly journals In vivo Neurological, Analgesic and In vitro Antioxidant and Cytotoxic Activities of Ethanolic Extract of Leaf and Stem Bark of Wedelia chinensis

2019 ◽  
Vol 22 (1) ◽  
pp. 18-26
Author(s):  
Sayema Khanum ◽  
Md Shahid Sarwar ◽  
Mohammad Safiqul Islam
Keyword(s):  
Body Weight ◽  
Oral Administration ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Pharmaceutical Journal ◽  
Stem Bark ◽  
Cytotoxic Activities

Wedelia chinensis is a widely used anti-inflammatory and hepatoprotective medicinal plant in Bangladesh. In this study, analgesic, neurological, antioxidant and cytotoxic activities of the ethanolic extract of leaf and stem bark of W. chinensis were investigated. Oral administration of the ethanolic extract of W. chinensis (200- and 300-mg/kg body weight) was investigated on animal model for neurological activity using open field test and hole cross test. Acetic acid induced writhing method was used to assess the analgesic activity. DPPH (1,1-diphenyl, 2-picryl hydrazyl) radical scavenging assay was used for determining the antioxidant activity, while brine shrimp lethality bioassay was used for investigating cytotoxicity. The ethanol extract of the plant produced significant reduction (P<0.05) of locomotion in both doses (200- and 300-mg/kg body weight) indicating pronounced neurological activity. Oral administration of alcoholic leaves and stem extracts significantly (p < 0.05) inhibited writhing response in mice. The percentage of scavenging of DPPH free radical was found to be concentration dependent with IC50 value of 44.10 ± 0.65 and 38.96 ± 0.50 μg/ml for leaves and stem extracts, respectively. Our findings indicate that W. chinensis may be a source of natural antioxidant with potent analgesic, neurological and cytotoxic activities. Bangladesh Pharmaceutical Journal 22(1): 18-26, 2019

scholarly journals Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
M Miftahudin ◽  
Rini Hasibuan ◽  
Tatik Chikmawati
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Sod Activity ◽  
Different Doses

Abstract. Miftahudin, Hasibuan RS, Chikmawati T. 2019. Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia. Biodiversitas 20: 3715-3722. Three Selaginella species, S. ornata, S. plana, and S. willdenowii, from Java Island, Indonesia, have been known to have antioxidant properties; however, in vivo antioxidant activities of these species have not been reported. This research aimed to evaluate the in vivo antioxidant activity of ethanolic extract of three Selaginella species. The 70% ethanol extract of three Selaginella species at four different doses was administered to mice one day before being treated with oxidative stress. The liver tissue of mice treated with or without oxidative stress was analyzed their lipid peroxidation by measuring MDA concentration and Superoxide Dismutase (SOD) activities. The results showed that there were variations in antioxidant activity among the three Selaginella species. In general, the dose of 0.3 g extract kg-1 BW has been able to reduce lipid peroxidation and increase SOD activity. The administration of S. ornata extract to the mice at 1.2 g extract kg-1 BW reduced the MDA concentration to the lowest level, but the same dose of two other Selaginella extracts caused toxic effects in mice. The antioxidant activities of S. ornata and S. plana were better than that of S. willdenowii extract, and among those species, S. ornata has the best antioxidant activity.

scholarly journals Secondary Metabolite Profiling, Anti-Inflammatory and Hepatoprotective Activity of Neptunia triquetra (Vahl) Benth

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7353
Author(s):  
Nasir Aziz Wagay ◽  
Shah Rafiq ◽  
Mohammad Aslam Rather ◽  
Younas Rasheed Tantray ◽  
Feng Lin ◽  
...  
Keyword(s):  
Total Protein ◽  
Ethanolic Extract ◽  
Liver Weight ◽  
Hepatoprotective Activity ◽  
Experimental Models ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Total Lipids ◽  
Anti Inflammatory

The present study aimed to analyze the phytoconstituents of Neptunia triquetra (Vahl) Benth. Anti-inflammatory and hepatoprotective activities of ethanol (EE), chloroform (CE) and dichloromethane (DCME) of stem extracts were evaluated using in vivo experimental models. The extracts were analyzed for phytoconstituents using GC-HRMS. Anti-inflammatory activity of CE, EE and DCME was accessed using carrageenan-induced paw oedema, cotton pellet-induced granuloma and the carrageenan-induced air-pouch model in Wistar albino rats. The hepatotoxicity-induced animal models were investigated for the biochemical markers in serum (AST, ALT, ALP, GGT, total lipids and total protein) and liver (total protein, total lipids, GSH and wet liver weight). In the in vivo study, animals were divided into different groups (six in each group) for accessing the anti-inflammatory and hepatoprotective activity, respectively. GC-HRMS analysis revealed the presence of 102 compounds, among which 24 were active secondary metabolites. In vivo anti-inflammatory activity of stem extracts was found in the order: indomethacin > chloroform extract (CE) > dichloromethane extract (DCME) > ethanolic extract (EE), and hepatoprotective activity of stem extracts in the order: CE > silymarin > EE > DCME. The results indicate that N. triquetra stem has a higher hepatoprotective effect than silymarin, however the anti-inflammatory response was in accordance with or lower than indomethacin.

scholarly journals Evaluation of potentials of aqueous ethanolic extract of Psidium guajava on reproductive functions of male Albino rats

2021 ◽  
Vol 19 (3) ◽  
pp. 174-181
Author(s):  
S.A. Abwage ◽  
S.T. Agu ◽  
S.A. Saganuwan ◽  
A.H. Abu
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Psidium Guajava ◽  
Reproductive Organs ◽  
Sperm Parameters ◽  
Albino Rats ◽  
Reproductive Parameters ◽  
Aqueous Ethanolic Extract ◽  
Ethanolic Leaf Extract

The therapeutic utilization of plants for managing several diseases by people of all continents, especially Africa, is as old as tradition. The objective of this study was to evaluate the potentials of aqueous ethanolic leaf extract of Psidium guajava on male reproductive parameters. Twenty-four male Albino rats were randomly assigned into four groups of six rats per group. Rats in the control group were administered Tween 20®. Rats in groups 2, 3, and 4 were administered Psidium guajava aqueous ethanolic leaf extract orally at the doses of 100, 200, and 400 mg/kg body weight, respectively, once daily for 60 days. The body weights of the rats were determined at the beginning and end of the experiment. Sperm parameters and some reproductive organs weight of each rat was also determined, some organs were collected for histopathology. Assay for follicle-stimulating hormone, luteinizing hormone, and testosterone was done using Enzyme-Linked Immunosorbent methods. Some reproductive organs were collected for histopathological analyses. The results showed a dose-dependent increase (p<0.05) in the weight of male reproductive organs, sperm parameters, and hormones. The extract significantly increased (P < 0.05) serum testosterone in the group treated 400 mg/kg body weight when treated groups were compared with control. Furthermore, the results of FSH and LH revealed a significant (p < 0.05) increase when the treated groups were compared with the control. The histopathological analysis did not reveal any form of damage to the architectural integrity of the testis. However, there was degenerating germinal epithelium in the group administered 400 mg/kg of the aqueous ethanolic leaf extract of P. guajava. Conclusion: aqueous ethanolic extract of P. guajava has positive effects on male reproductive parameters.

scholarly journals Effect of Phyllanthus amarus on Some Reproductive Indices of Male Albino Rats

2019 ◽  
pp. 1-8
Author(s):  
P. B. Ekpo ◽  
N. E. Edu ◽  
A. J. Umoyen ◽  
T. L. Thomas ◽  
S. O. Abraham
Keyword(s):  
Medicinal Plants ◽  
Sperm Count ◽  
Reproductive Organs ◽  
Male Rats ◽  
Albino Rats ◽  
Phyllanthus Amarus ◽  
Experimental Animals ◽  
Treatment Groups ◽  
Randomized Design ◽  
Group B

Background: Medicinal plants have been a good source of drugs for humans, but chronic and prolong use of medicinal plants like Phyllanthus amarus for the treatment of malaria and other disorders are issues of concerns. This study evaluated the effect of Phyllanthus amarus on reproductive organs and sperm parameters in albino rats. Materials and Methods: Twenty-four healthy male albino rats of 12 weeks old were assigned into four groups with six rats in each group using a Completely Randomized Design (CRD). The experimental animals were orally treated with Phyllanthus amarus. Group A served as the control and was given only water and feed; Group B, C and D  received 100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW of Phyllanthus amarus respectively. Data obtained were analyzed using analysis of variance (ANOVA). The treatments lasted for a period of 65 days after two weeks of acclimatization. Results: The results showed statistically significant (p<0.05) reduction in weight of testes and epididymes, sperm motility, sperm viability, sperm count and sperm head abnormalities in male  rats treated with Phyllanthus amarus when compared to the control. The sperm pH was not significantly (p>0.05) affected by Phyllanthus amarus among the different treatment groups in the experimental animals. Conclusion: Findings from the present study indicate that Phyllanthus amarus possesses a dose-dependent anti-fertility activity in amale albino rats under a sub-chronic course of administration.

scholarly journals Assessment of Nephroprotective Potential of Ethanolic Extract Premna Tomentosa (Ept) in Alcohol Toxicity in Male Albino Rats

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 3099-3103
Author(s):  
Gomathi Priyadharsini T ◽  
Kavimani M ◽  
Sathiya Narayana Murthy S ◽  
Prabhu K ◽  
Arudyuti Chowdhury
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Blood Parameters ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Histopathological Changes

The nephroprotective prospective of Premna tomentosa extract against Alcohol induced nephrotoxicity in rats was investigated in the present study. The characterization of ethanol extract of Premna tomentosa (EPT) was performed using standard phytochemical analysis. Male albino wistar rats 36 in numbers were divided into 6 groups including control, negative control, positive control and various doses of EPT Treated groups; Nephrotoxicity was induced by alcohol (1ml/100gm b.wt) in animals. Rats intoxicated with Alcohol were fed with 500, 750 mg/kg dose of EPT and Liv 52 (1ml/100gm b.wt) for 60 days. Results show that EPT (500mg/kg b.wt) had a significant effect against alcohol induced nephrotoxicity in rats than EPT (750mg/kg b.wt) in blood parameters and consonantly good histopathological changes in kidney. The deleterious histopathological alterations in kidney associated with glomerular and tubular changes in alcohol intoxicated rats was evident. This result shows Premna tomentosa may be used as supplementary drug for alcoholics.

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