scholarly journals The effect of colostral supplement on the serum protein fractions, health status and growth of calves

2011 ◽  
Vol 54 (2) ◽  
pp. 115-126 ◽  
Author(s):  
M. Szewczuk ◽  
E. Czerniawska-Piątkowska ◽  
S. Palewski
Keyword(s):  
Total Protein ◽  
Gamma Globulin ◽  
Poor Quality ◽  
Favourable Effect ◽  
Control Group ◽  
Health State ◽  
Globulin Fraction ◽  
Black And White ◽  
Time Period ◽  
Holstein Friesian

Abstract. The study aimed at evaluation of the effect of colostral supplement on humoral immunity, growth and health state of calves until the 3rd month of life. Examinations were carried out on 40 Polish Black-and-White Holstein-Friesian calves. After birth, the calves were separated into two groups (20 calves each): control group – without addition of colostral supplement, and experimental one – with colostral supplement in diet. Blood was sampled from each calf on day 5 and 30 of life, and total protein, albumin, and alpha, beta and gamma globulin levels were determined in it. Evaluation of calves’ growth and development course was carried out basing on weighing and daily gain results. The favourable effect of colostral supplement on calves’ health state and body weight gains was found. No significant effect of colostral supplement on total protein level or gamma-globulin fraction level was found. The effect of colostral supplement on reduction of alpha-globulin fraction decrease was found in a time period until the 30th day of calves’ life (P≤0.01). The study so far point to the purposefulness of using this supplement, in particular in the case of colostrum deficiency or its poor quality.

INFLUENCE OF IMMUNOSTIMULATORS ON MORPHOLOGICAL AND IMMUNOBIOCHEMICAL PARAMETERS OF BLOOD OF PREGNANT COWS AND NEWBORN CALVES

2020 ◽  
Vol 36 (2) ◽  
pp. 215-223
Author(s):  
O.M. Altynbekov ◽  
◽  
A.V. Andreeva ◽  
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Gamma Globulin ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Control Group ◽  
Maximum Difference ◽  
Black And White ◽  
Newborn Calves

The article reflects the results of changes in the biochemical and morphological parameters of blood, as well as the content of total protein and its fractions under the influence of immunostimulators in black-and-white cows and calves obtained from them. The aim of the study was to study the effect of the immunostimulators “Recombinant Bovine Interferon” and “Immunate” on the morphological and immunobiochemical blood parameters of pregnant cows and newborn calves. Studies were conducted on 45 pregnant cows divided into three groups based on the principle of analogues, and, subsequently, on 45 calves obtained from mother cows and divided into nine groups. Maternal cows during pregnancy and newborn calves were given immunostimulators in various combinations. In the blood of cows of the experimental groups, five days before calving, higher indicators of red blood cells and hemoglobin were registered in comparison with the control indicators. Also, in experimental cows, the content of total protein and globulins exceeded the indicators of the control group. Calves received from cows immunostimulated with “Recombinant Bovine Interferon” and who were given “Immunate” after birth had the smallest decrease in the number of red blood cells and white blood cells on the tenth day. After drinking colostrum in calves of experimental groups, the total amount of protein increased, especially there was an increase in the gamma-globulin fraction. For all periods of the study, the content of immunoglobulins A, M, G in the blood serum of calves of the control group was lower than that of calves of the experimental groups. The maximum difference between these indicators and the control was found in animals of the sixth and ninth groups.

Effects of Repeated Bleedings on Serum Glycoprotein Concentrations in the Guinea Pig

1957 ◽  
Vol 190 (3) ◽  
pp. 529-532 ◽  
Author(s):  
Henry E. Weimer ◽  
Frances A. Quinn ◽  
Hisako Nishihara
Keyword(s):  
Blood Loss ◽  
Guinea Pig ◽  
Adult Male ◽  
Total Protein ◽  
Guinea Pigs ◽  
Control Group ◽  
Globulin Fraction ◽  
Serum Concentrations ◽  
Serum Glycoprotein ◽  
Experimental Group

Effects of repeated bleedings on the serum concentrations of total glycoprotein, seromucoid, albumin polysaccharide, globulin polysaccharide, total protein, albumin and globulin and on the hematocrit and hemoglobin values of blood were investigated in adult male guinea pigs. Significant increases occurred in the seromucoid fraction and in the carbohydrate and protein moieties of the globulin fraction. Significant decreases occurred in albumin polysaccharide, hemoglobin and hematocrit values. Statistical comparisons of the final values of the experimental group with those of a control group subjected to the same experimental procedures but without major blood loss were significant for total protein, albumin, hemoglobin and hematocrit. The conclusion was drawn that the alterations which occurred in the serum glycoprotein concentrations were not due primarily to blood loss.

scholarly journals Protein synthesize function of bulls liver at experimental chronic cadmium toxicity

2019 ◽  
Vol 21 (94) ◽  
pp. 92-96 ◽  
Author(s):  
Y. Y. Lavryshyn ◽  
B. V. Gutyj
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Cadmium Chloride ◽  
Cadmium Toxicity ◽  
The Body ◽  
Control Group ◽  
Black And White ◽  
Wide Range ◽  
Inflammatory Processes ◽  
Experimental Group

The article presents the results of studies on the influence of cadmium load on the body of young cattle. The adverse effect of Cadmium on the liver causes a wide range of pathological changes at various levels of its organization. The purpose of the work was to investigate protein synthesize function on the liver of bulls for experimental chronic cadmium toxicity. The research was carried out on the basis of a farm at the village Ivanivtsi, Zhydachiv district of Lviv region, on 10 bulls of six months old, Ukrainian black-and-white milk breed, which were formed in 2 groups of 5 animals in each: control and experimental. Bulls of the control group were in the usual diet. Bulls of the experimental group were fed with forage with cadmium chloride at a dose of 0.04 mg/kg body weight of the animal. The research lasted for 30 days. Protein synthesize function of the liver was determined by the level of serum total protein and protein fractions. It was established that when feeding cows with feed of cadmium chloride in a dose of 0.04 mg/kg of body weight of an animal for 30 days in animals suppressed protein synthesize function of the liver, which manifests itself by lowering the total protein of blood. It reached the lowest level at the 20th day of the experiment, where, accordingly, it fluctuated within the limits of 61.9 ± 1.14 g/l. Reducing the level of total protein in the blood of the bulls of the experimental group under cadmium loading was due to lowering the level of albumin. At day 20 of the experiment, the level of albumin in the blood of animals in the experimental group decreased by 16.5%. On the background of general hypoproteinemia there is a significant imbalance between albumins and globulins in serum of diseased animals. It was established that the level of globulins increased in the blood of Bulls, which were under cadmium load. Increasing the level of globulins in blood serum of bulls reflects the intensity of inflammatory processes in their body for cadmium loading.

scholarly journals The effect of a feed additive supplemented with porcine plasma protein on growth performance and selected biochemical indices of nursing piglets

2019 ◽  
Vol 88 (4) ◽  
pp. 393-399
Author(s):  
Balázs Kósa ◽  
Katarína Bárdová ◽  
Lenka Lešková ◽  
Peter Reichel ◽  
Jaroslav Novotný ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Total Protein ◽  
Feed Additive ◽  
Control Group ◽  
Health State ◽  
Feed Mixture ◽  
Significant Difference ◽  
Weight Gains ◽  
Biochemical Indices ◽  
Experimental Group

The aim of this study was to evaluate the health state, performance, and selected serum biochemical indices in nursing piglets after supplementation of a feed additive containing porcine plasma protein in comparison to the control group fed a standard pre-starter feed mixture. The study was carried out in the conditions of industrial farming. Four sows of the Large White swine breed with their litters were included in the study. Piglets were homogenously distributed into control (22) and experimental (24) groups according to weight and sex. Piglets in both groups suckled their mothers' milk. In addition, piglets in the experimental group were also fed a feed supplemented by porcine plasma protein (5%), starting on the third day post partum, whereas piglets in the control group were offered a standard pre-starter feed mixture. We recorded average daily weight gains calculated per the 27-day trial, and body weight at weaning. Clinical findings, mortality, and selected biochemical indices of protein (total immunoglobulins, total protein, albumin, creatinine, urea), and energy (glucose, total cholesterol, total lipids) metabolism were assessed as well. Results of the study showed a significant difference (P < 0.01) in average daily weight gains in the experimental group compared to the control group (0.245 kg and 0.177 kg, respectively). Addition of plasma protein in the experimental group also resulted in a significantly higher serum concentration of total immunoglobulins, total protein and creatinine, and a significantly lower concentration of urea and albumin in this group compared to the control group at weaning.

scholarly journals The effect of Leiber Beta-S on selected immunity indicators in calves

2014 ◽  
Vol 83 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Roman Wójcik
Keyword(s):  
Respiratory Burst ◽  
Total Protein ◽  
Proliferative Response ◽  
Gamma Globulin ◽  
Burst Activity ◽  
Control Group ◽  
Respiratory Burst Activity ◽  
Killing Activity ◽  
Cellular And Humoral Immunity ◽  
Ceruloplasmin Activity

A wide variety of dietary supplements containing polysaccharides are being introduced on the market. One of them is Leiber Beta-S (β-1,3/1,6-D-glucan) whose immunostimulatory effects have not yet been fully evaluated, in particular in polygastric animals. The aim of this study was to evaluate the effect of this supplement on selected indicators of cellular and humoral immunity in calves. The experiment was performed on 14 calves aged 30 ± 2 days, divided into two equal groups of control and experimental animals. The feed administered to calves of the experimental group was supplemented with Leiber Beta-S at 50 mg/kg body weight, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled before the experiment (day 0) and on days 15, 30 and 60 to determine the immunity indicators (proliferative response of lipopolysaccharide- and concanavalin A-stimulated lymphocytes, respiratory burst activity, potential killing activity of phagocytes, gamma globulin concentrations, lysozyme activity, ceruloplasmin activity) and biochemical indicator (total protein concentrations). Diet supplemented with Leiber Beta-S stimulated the immune system of calves. Significant differences between the experimental and the control group were found in lysozyme and ceruloplasmin activity, gamma globulin concentrations, potential killing activity of phagocytes, proliferative response of lymphocytes (P < 0.001) and respiratory burst activity of phagocytes (P < 0.05). No differences were found in the serum total protein between the experimental and the control group. This study reports for the first time the effect of Leiber Beta-S (β-1,3/1,6-D-glucan) on selected biochemical and immunity indicators in calves.

PROTEIN LEVELS IN SERUM OF PREMATURE INFANTS FED DIETS VARYING IN PROTEIN CONCENTRATIONS

PEDIATRICS ◽  
1962 ◽  
Vol 30 (4) ◽  
pp. 622-628
Author(s):  
Joseph B. Pincus ◽  
Isaac F. Gittleman ◽  
Emanuel Schmerzler ◽  
Norma Brunetti
Keyword(s):  
Human Milk ◽  
Premature Infants ◽  
Plasma Protein ◽  
Total Protein ◽  
Gamma Globulin ◽  
Globulin Fraction ◽  
Albumin Fraction ◽  
Protein Levels

The concentration of the protein of the diet of artificially fed infants influences the concentration of the total protein and the albumin fraction of the serum. The composition of the protein of the diet, i.e., the lactalbumin: casein ratio, may also influence the plasma protein levels. The globulin fraction decreases significantly in the artificially fed groups but not in the group receiving human milk. The gamma-globulin decreases significantly in all groups studied, but globulins alpha1, alpha2, and beta do not change significantly from day 1 to day 21.

Effect of opioid peptides on liver function after patial hepatectomy

2018 ◽  
pp. 67-71
Author(s):  
А.В. Солин ◽  
А.Ю. Ляшев ◽  
Ю.Д. Ляшев
Keyword(s):  
Lactate Dehydrogenase ◽  
Liver Failure ◽  
Partial Hepatectomy ◽  
Opioid Receptors ◽  
Total Protein ◽  
Pronounced Effect ◽  
Male Rats ◽  
Control Group ◽  
Peptide Molecule ◽  
Selective Agonists

Цель исследования - сравнительный анализ влияния селективных агонистов отдельных классов опиоидных рецепторов на белковосинтетическую функцию печени, развитие цитолитического и холестатического синдромов у крыс, подвергшихся частичной гепатэктомии. Методика. Работа выполнена на 152 крысах-самцах Вистар массой 200-250 г. Частичную гепатэктомию выполняли по методу, описанному Higgins G.M. и Anderson R.M. с удалением 70% ткани печени. В плазме крови определяли концентрации общего белка, альбуминов, общего билирубина, активность аланинтрансаминазы (АЛТ), аспартаттрансаминазы (АСТ), лактатдегидрогеназы (ЛДГ) традиционными методами. Опиоиды: DAGO в дозе 6,3 мкг/кг, DSLET в дозе 10,0 мкг/кг, динорфин А (1-13) в дозе 20,1 мкг/кг, вводили внутрибрюшинно ежедневно 1 раз в сутки в течение 5 сут. эксперимента в объеме 0,2 мл. Контрольным животным аналогично вводили физраствор. Результаты. Удаление 70% ткани печени у крыс-самцов Вистар сопровождается развитием печеночной недостаточности, проявляющейся гипербилирубинемией, гипоальбуминемией, гипопротеинемией, повышением активности трансаминаз и лактатдегидрогеназы. Применение селективных агонистов опиоидных рецепторов у крыс, которым моделировали частичную гепатэктомию, оказывало гепатопротективное действие и снижало выраженность проявлений печеночной недостаточности, начиная с 3-х сут. после резекции. Активность трансаминаз, лактатдегидрогеназы и концентрация общего билирубина у животных, которым вводили опиоиды, были существенно ниже, чем в контрольной группе. Содержание общего белка и альбуминов было статистически значимо выше в группах, которые получали исследованные пептиды, по сравнению с контрольной группой на 7-е сут. после частичной гепатэктомии. Наиболее выраженное действие проявлял селективный агонист опиоидных мю-рецепторов DAGO. По нашему мнению, такое влияние пептидов объясняется присущими им антиоксидантным и антигипоксическим эффектами, что снижает повреждающее действие оперативного вмешательства на печень. Более выраженное влияние DAGO связано, по-видимому, с особенностями распределения опиоидных рецепторов или устойчивостью пептида к действию эндопептидаз благодаря модификациям в молекуле пептида. Заключение. Применение опиоидов стимулирует восстановление функциональной активности печени после частичной гепатэктомии. Наибольший эффект отмечается при введении мю-агониста DAGO. Aim. The aim of the study was to compare effects of selective agonists of opioid receptors from different classes on the protein-synthesizing function of liver and development of cytolytic and cholestatic syndromes in rats after partial hepatectomy. Methods. The study was conducted on 152 Wistar male rats weighing 200-250 g. The animals were subjected to partial hepatectomy according to the Higgins and Anderson method. Concentrations of total protein, albumin, total bilirubin, and activities of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase were measured in plasma using standard methods. The opioids, DAGO (6.3 mg/kg), DSLET (10.0 mg/kg), and dynorphin A (1-13) (20.1 mg/kg), were injected in 0.2 ml of saline daily for 5 days. Control animals were injected with 0.2 ml of saline for 5 days. Results. Resection of 70% of liver tissue resulted in development of liver failure as evidenced by hyperbilirubinemia, hypoalbuminemia, hypoproteinemia, and increased transaminase and lactate dehydrogenase activities. Selective agonists of opioid receptors administered to the rats after partial hepatectomy exerted a hepatoprotective effect and alleviated the signs of liver failure beginning from the 3 day after resection. Transaminase and lactate dehydrogenase activities were significantly lower in opioid-treated rats than in the control group. Levels of total protein and albumins were significantly higher in the groups injected with the study peptides compared to the control group on the 7 day after partial hepatectomy. The selective agonist of opioid m-receptors, DAGO, exerted the most pronounced effect. Apparently, the similar effects of peptides were due to their antioxidant and anti-hypoxic action, which alleviated the detrimental effect of liver surgery. The more pronounced effect of DAGO apparently resulted from peculiarities of opioid receptors distribution or peptide resistance to endopeptidase action due to modifications of the peptide molecule. Conclusion. Administration of opioids stimulated restoration of liver functional activity after partial hepatectomy. Injections of the m-agonist, DAGO, produced the most pronounced effect.

The influence of probiotic drug Biolax-U on morphological and biochemical parameters of blood in cows

2020 ◽  
pp. 70-76
Author(s):  
A. Farkhutdinova
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Experimental Studies ◽  
Positive Influence ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Animal Blood ◽  
Black And White ◽  
White Breed

The expediency of using the probiotic drug Biolax-U in the rations of lactating and dry cows of Black-and-White breed has been studied and scientifically proved. The influence of this drug on the morphological and biochemical parameters of animal blood has been determined. The composition of the probiotic drug Biolax-U consists of eight strains of a balanced complex of living lactic acid bacteria, yeast and actinomycetes, as well as biologically active substances. For experimental studies conducted on 2 groups of cows have been selected by the method of pairs-analogs, the influence of feeding of feed have been prepared with the use of the microbiological drug with probiotic action Biolax-U on feed consumption, morphological and biochemical changes in blood has been studied. Analysis of the rations of cows indicates that the content of calcium, phosphorus, magnesium, sulfur and trace elements such as manganese, copper, zinc and cobalt in the feed has been reduced. It has been found by studies that the use of probiotic drug Biolax-U had the positive influence on the feed intake, digestibility and better assimilation of feed nutrients and contributed to the improvement of blood morphological and biochemical parameters. For example, in animals of the experimental group hemoglobin has increased by 3,6 % (Р < 0,05), red blood cells by 10,1 (P ≤ 0,05), the content of leukocytes by 4,43, carotene by 9,6, glucose by 3,4; reserve alkalinity by 15,6, calcium by 19,4 (P ≤ 0,01), and phosphorus by 18,4 % compared with the control group of animals. During the lactation significantly improved hematological parameters including alkalinity reserve by 5,11 %, calcium level by 11,1, phosphorus by 8,6, hemoglobin by 14,2 %, the content of erythrocytes by 12,3, leukocyte by 3,86, and carotene 11,1 % against the parameters of animals of control group. The improvement of morphological and biochemical parameters of blood of cows indicates about stimulating effect on hematopoiesis and the general clinical condition of animals that contributes to the improvement of metabolic processes when using feed prepared with the use of microbiological drug with probiotic action Biolax-U.

Extruded lupine as a part of energy-protein concentrate in the ration of lactating cows

2020 ◽  
pp. 65-73
Author(s):  
Z. Fedorova
Keyword(s):  
Farm Animals ◽  
Feed Consumption ◽  
Control Group ◽  
Protein Concentrate ◽  
Cattle Farm ◽  
Black And White ◽  
Lactating Cows ◽  
White Breed ◽  
Kaliningrad Region ◽  
Experimental Group

In order to fi ll the existing protein defi ciency in the rations of farm animals in particular dairy cows it is necessary to input feed lupins as widely as possible in crop rotations. Lupin is a very versatile forage crop it can be used in feed as herbage, in the form of haylage, silage, in compound feed as a protein additive, as well as a green manure crop to increase soil fertility. Questions of protein nutrition should be to be quickly resolved, both in the production of protein and in its standardized rational use. Unbalanced protein rations increase feed consumption per unit of production, thereby increasing its prime cost. In the world soya is generally recognized as a protein feed, but in the Kaliningrad region as in most of regions in our country, it does not maturate being a heat-loving crop. Under these environments the inclusion of extruded lupine grain in the composition of energy-protein concentrates for the production of compound feed has become an alternative to replacing expensive soya. The researches have been carried out it the cattle farm of LLC “Temp” in the Guryevsky area in the Kaliningrad region (March-May 2019) on feeding lactating cows energy-protein concentrate from extruded grain of narrow-leaved lupine, fl ax, triticale, subjected to heat treatment on a press extruder. For this purpose two groups of lactating cows of Black-and-White breed (control and experimental) have been formed using the method of pairs of analogs per 11 heads in each. Cows of the control group have received the main ration of the farm, and cows of the experimental group in addition to the main ration have received compound feed with extruded energy-protein concentrate based on lupine. As a result of research, the positive infl uence of energy-protein concentrate based on lupine in compound feed on the dairy productivity of cows has been established. Recipes of concentrate and compound feed with concentrate for lactating cows, the volume of inclusion of components in the concentrate and in the compound feed have been calculated. During the experiment, the milk yield in the experimental group of cows has increased by 62,4 kg or 8,0 %, than in the control group. From the cows of the experimental group the conditional profi t has been received in the amount of 1684,8 rubles per head.

The effectiveness of use RUSMA additive in the rations of lactating cows

2020 ◽  
pp. 28-37
Author(s):  
S. Sukhanova ◽  
N. Pozdnyakova ◽  
F. Yaroslavtsev
Keyword(s):  
Milk Fat ◽  
Raw Materials ◽  
Fat Content ◽  
Feed Additives ◽  
Control Group ◽  
New Mineral ◽  
Milk Productivity ◽  
Black And White ◽  
Lactating Cows ◽  
Experimental Group

The results of researches on the development of new mineral feed additives based on raw materials produced by Uralchem and the study of the eff ectiveness of their use in the rations of lactating cows have been presented in the article. The experiment has been carried out in the conditions of LLC “PF Barabinskoe” in the Dalmatovsky area in the Kurgan region where 3 groups of lactating cows of Black-and-White breed have been formed. Animals in the control group have been received the ration adopted in the farm, the 1st experimental group with the additive RusMA No. 1 at a dose of 150 g, the 2nd experimental group– with the additive RusMA No. 2 at the dose of 350 g. The use of experimental mineral additives in feeding lactating cows allowed us to increase their milk productivity and profi tability of production. At the same time, the best eff ectiveness was shown by the additive RusMA No. 2 at the dose of 350 g/head/day. The use of RusMA additives during the experiment period (105 days) allowed to increase the milk productivity of cows in the 1st experimental group by 6,03 % and in the 2nd experimental group by 8,24 %. Taking into account the fat content in milk, this diff erence was 6,31 and 8,53 %, respectively. The energy value of milk from cows of the experimental groups was higher compared to the control group by 2,45 and 1,76 %, respectively. According to the content of dry matter in milk, cows of the 2nd experimental group exceeded the control and 1st experimental group by 0,39 and 0,12 abs.%, respectively. The maximum milk fat content has been observed in the milk of cows of the 1st experimental group by 0,03 and 0,01 abs.% more than in the control and 2nd experimental groups, respectively. In terms of protein content, the cows of the 2nd experimental group signifi cantly exceeded the control group by 0,10 abs.% and the 1st experimental by 0,04 abs.%. The content of milk sugar in the 2nd experimental group was higher than in the control by 0,30 abs.% and compared to the 1st experimental by 0,11 %. Signifi cantly more calcium and phosphorus were contained in the milk of cows of the 2nd experimental group by 4,58 % (Р ≤ 0,001) and 2,86 % (Р ≤ 0,05), compared with the control group. The expenditures of feed in the EFU for the production of 1 kg of milk in animals of the 2nd experimental group was less by 5,13 % than in control cows and by 2,63 % compared to the 1st experimental group. The prime cost of 1 centner of milk was lower in the 2nd experimental group by 3,94 %, in the 1st experimental group by 2,65 % compared to the control group. The level of profi tability of milk production in the 2nd experimental group was higher by 9,0 and 1,0 abs.% in comparison with the control and 1st experimental groups, respectively.

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