scholarly journals Germination energy and germination of different morphotypes of peas when treated with growth stimulant

Author(s):  
O. P. Oreshnikova ◽  
E. V. Kozhukhova

The main legume crop, pea, is characterised by various morphotypes: leafy, whiskered, chameleon and other morphotypes. These morphotypes are the result of both selection work and spontaneous mutations. The relevance of the research topic is the effect of various factors, including seed treatment, on the different morphotypes. Six varieties and breeding lines of pea sown by Krasnoyarsk Research Institute of Agriculture were taken for the study: leafy Radomir, Kemchug; with the moustached leaf D-94, Ruslan; and having longitudinal heterophyllia L-19, M-6. This work aims to determine the responsiveness and variability of germination and germination energy, as well as seedling length, recorded during these periods, of different morphotypes of pea varieties and breeding lines to seed treatment with a growth stimulant. The experiment was set up in three replications with the growth stimulant treatment sodium humate and three repetitions of the control variant without remedy. Measurements of seedling length, germination energy and germination were carried out on the 4th and 8th day, respectively. The research revealed that the treatment of pea seeds increased the germination energy by 2,28% and reduced its variation by 0,55%. Chameleon morphotype samples are more responsive to treatment. The variability of the coefficient of variation of the germination energy of pea plants of different morphotypes with and without treatment is less than 10%. Stimulant treatment increases the length of seedlings by 6.34% but does not affect the morphotypes separately. The increase in size at treatment is individual for each sample. The variability of variation in the length of seedlings of control and treated examples is insignificant and unique for each model. But this variability in size decreases throughout the experiment: on the 4th day from 1.63 to 1.36, on the 8th day from 0.98 to 0.95%. In determining the germination rate, it was found that the treatment of pea seeds with a growth stimulant increases it by 3.78%. The growth gain is an individual response of each specimen and is weakly dependent on the morphotype.

Author(s):  
Muhammad Jaffer Ali ◽  
Zheping Yu ◽  
Guangnan Xing ◽  
Tuanjie Zhao ◽  
Junyi Gai

The experiments concluded 48hrs for germination testing as most appropriate stress duration. Using paper roll for recording the traits was the best method than petri dish and towel method. Among the traits relative germination rate (RGR), relative seedling length (RSL), relative root length (RRL), relative root fresh weight (RRFW) and relative root dry weight (RRDW) were proved to be flooding-responsive. RSL was chosen as the major seed-flooding indicator due to higher heritability (h2), genotypic coefficient of variation (GCV), correlation with other indicators and easier measuring procedure, while RRL was considered as the subsidiary indicator. Thus, the RSL in paper roll after 48hrs seed-flooding was recognized as standard seed-flooding testing procedure and used to evaluate breeding materials. Among the 11 cultivars, the superior seed-flooding-tolerant ones were M8206, NN1138-2 and ZXD, while ten best lines were evaluated from one hundred breeding lines.


2020 ◽  
pp. 34-37
Author(s):  
Tatyana Yakovlevna Prakhova ◽  
Anna Nikolaevna Kshnikatkina ◽  
Vladimir Aleksandrovich Prakhov ◽  
Sergey Ivanovich Korzhov

The article presents the results of studies evaluating the influence of growth stimulants and micronutrient fertilizers on the productivity and sowing qualities of Crambe Abyssinica. Growth regulators Albit - 40 ml/t, Gumat K/Na - 0.15 l/t and microelement fertilizers EcoFus - 0.5 l/t and Omex - 2 l/t were studied. Studies have shown that the most effective options are with seed treatment with the growth regulator Albit and the combined use of Albit + Omex. Here the greatest positive effect was obtained on the indicator of field germination of krambe (89.6%) and on the safety of plants for harvesting (96.8%), respectively. It was revealed that the variant with the use of Albit, Gumat K/Na + Omex and Albit + Omex most effectively increased the seed yield of Crambe, the seed productivity here was 2.92; 2.93 and 2.96 t / ha, respectively.  These options stimulated germination energy by 1.8-4.7% and laboratory germination by 3.8-9.1% relative to the control. The krambe oil content in the experimental variants was 36.14-39.76%, in the control - 36.04%. The highest oil content of seeds was noted on the variant with Albit - 39.76%. The use of Albit in combination with EcoFus and Omex contributes to the accumulation of oil in seeds up to 38.55 and 38.68%, respectively. The weight of 1000 seeds of Crambe varied in the range of 8.16-8.38 g in the variants with the use of drugs and 8.13 g in the variant without treatment. The germination energy and laboratory germination rate of Crambe Abyssinica varied between 52.7-79.8% and 69.5-86.5%, depending on the use of the preparations.


2018 ◽  
Vol 78 ◽  
pp. 51-61
Author(s):  
Mehdi Aghighi Shahverdi ◽  
Heshmat Omidi

In order to determine anoptimal duration and concentration of priming of stevia seed with boric acid for improvinggermination, an experimental factorial completely randomized design with three replications was conducted inthe laboratory of Seed Science and Technology, Shahed University of Tehran in 2015. The first factor was differentconcentrations of boric acid (zero, 0.5, 1, 1.5 and 2 percent) and the second factor of priming time (0, 8, 16, 24 and 32hours). Effects of concentration and duration of priming with Boric acid was significant on germination percentage,germination mean time, germination rate, germination energy, germination uniformity, germination mean daily,germination daily rate, germination value, seedling length and seed vigor index. The highest germination percentage,germination rate, germination energy, germination mean daily germination value and seed vigor index was 24 hourspriming and the highest germination percentage, germination rate, germination potential, germination value and seedvigor index in priming of 2% Boric acid were obtained. Germination uniformity highest in 24 hours at 1% concentrationpriming of boric acid (5.51). Seedling length in 24 hours at 1.5% concentration priming with boric acid washighest average 1.02 cm. Generally Stevia seed priming with boric acid for 24 hours at concentrations of 1.5 to 2percent had positive effects on germination indexes and seedling growth.


Author(s):  
Eduardo José Ludwig ◽  
Ubirajara Russi Nunes ◽  
Osmar Damian Prestes ◽  
Lovane Klein Fagundes ◽  
Tiéle Stuker Fernandes ◽  
...  

This study evaluated the physiological quality and quantified the efficiency of polymer in reducing the loss of phytosanitary products by leaching from soybean seeds of different diameters and in different treatments. Two lots of seed and two types of treatments were tested: polymers associated with seed treatment, and polymer application in layers after the seed treatment. For that, the following treatments were analyzed: Control= no seed treatment; Treat+pol= seed treatment + polymers in the mix; Treat/pol= seed treatment + polymers applied in layers; Treat= seed treatment. To evaluate seed treatment retention by the polymers, extraction equipment was employed and leaching of the active ingredient thiamethoxam was quantified. Evaluation of physical quality was conducted with tests of germination rate, first count, emergence rate, accelerated aging, seedling length, seedling dry mass and moisture content. Polymer application associated with seed treatment was efficient in reducing losses by leaching, reducing product loss by 20%. No significant differences in retention and physiological quality were found when different methods for polymer application were compared. Bigger seeds presented greater vigor, but the germination rate was unaffected.


2020 ◽  
Vol 10 (5) ◽  
pp. 1685-1696
Author(s):  
Lorenzo Stagnati ◽  
Vahid Rahjoo ◽  
Luis F. Samayoa ◽  
James B. Holland ◽  
Virginia M. G. Borrelli ◽  
...  

Fusarium verticillioides, which causes ear, kernel and stem rots, has been reported as the most prevalent species on maize worldwide. Kernel infection by F. verticillioides results in reduced seed yield and quality as well as fumonisin contamination, and may affect seedling traits like germination rate, entire plant seedling length and weight. Maize resistance to Fusarium is a quantitative and complex trait controlled by numerous genes with small effects. In the present work, a Genome Wide Association Study (GWAS) of traits related to Fusarium seedling rot was carried out in 230 lines of a maize association population using 226,446 SNP markers. Phenotypes were scored on artificially infected kernels applying the rolled towel assay screening method and three traits related to disease response were measured in inoculated and not-inoculated seedlings: plant seedling length (PL), plant seedling weight (PW) and germination rate (GERM). Overall, GWAS resulted in 42 SNPs significantly associated with the examined traits. Two and eleven SNPs were associated with PL in inoculated and not-inoculated samples, respectively. Additionally, six and one SNPs were associated with PW and GERM traits in not-inoculated kernels, and further nine and thirteen SNPs were associated to the same traits in inoculated kernels. Five genes containing the significant SNPs or physically closed to them were proposed for Fusarium resistance, and 18 out of 25 genes containing or adjacent to significant SNPs identified by GWAS in the current research co-localized within QTL regions previously reported for resistance to Fusarium seed rot, Fusarium ear rot and fumonisin accumulation. Furthermore, linkage disequilibrium analysis revealed an additional gene not directly observed by GWAS analysis. These findings could aid to better understand the complex interaction between maize and F. verticillioides.


2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.


Author(s):  
O. V. Chernikova ◽  
L. E. Ampleeva ◽  
Yu. A. Mazhaisky

Peculiarities of yield formation of maize variety Obskiy 140 the cultivation of it with the use of selenium nanoparticles of an optimum concentration. The experiment was carried out in soil culture in 3-fold repetition. Agrochemical parameters of soil: рН 6,2; the КСl humus of 2.6%; P O 204 mg/kg and K O 219 mg/kg soil. Four variants of the experiment were developed: control 1 (dry seeds), 2 5 2 control 2 (soaking seeds in water), NP Se inlay (semi-dry etching) and NP Se soaking (30 minutes before sowing at the rate of 0,1 g/ha of NP selenium). In the experiments, NP Se containing 3,7 mg of nanopreparation Se in 1 liter of water was used. The positive effect of selenium nanoparticles on the growth processes of maize seeds: germination, germination energy, growth force is shown. Se NP seed treatment increases germination energy by 6,90 and 9,13%, laboratory germination by 4,67 and 8,34%, growth strength by 26,32 and 31,05%, respectively, control 2 (soaking in water) and control 1 (dry change). Under adverse conditions, the use of selenium nanoparticles increases the growth of plants in height. Positive effect on the accumulation of starch in corn grains inlay NP Se, and soaking in this drug increases the content of protein fractions. The greatest increase in the yield of dry corn phytomass provides soaking seeds with selenium nanoparticles, which is caused by an increase in the yield of green corn mass.


2011 ◽  
Vol 50 (No. 11) ◽  
pp. 519-527 ◽  
Author(s):  
R. Dvořák ◽  
A. Pechová ◽  
L. Pavlata ◽  
J. Filípek ◽  
J. Dostálová ◽  
...  

The goal of the trial was to reduce the content of antinutritional substances in pea (Pisum sativum L.) seeds in order to enhance its use in livestock nutrition. A variety of field pea (Pisum sativum L.) with a high content of antinutritional substances and favourable production traits (Gotik) was chosen. Native and heat-treated pea seeds were used to collect representative samples (n = 6) for analytical purposes. The technology (V-0 technology, Czech patent No. 285745) was further modified by adjusting the reactor temperature, the duration of exposure to that temperature, and the duration of ageing of the material treated in this way (V-I and V-II technologies). The methodology of treatment is based on exposing pea seeds to vapour, organic acids and selected oxides.The monitored parameters included antinutritional substances. As far as the antinutritional substances were concerned, the content of trypsin inhibitors in native pea seeds (P) was around 15.4 ± 0.5 TIU. After treatment with technologies V-0, V-I, and V-II its activity dropped by 83.8, 80.5 and 83.8%, respectively. The pre-treatment titre of lectins (P) was 717 ± 376. It dropped by 70.3, 35.7 and 73.2% after treatment with technologies V-0, V-I and V-II, respectively. The content of tannins measured by the amount of gallic acid in native pea seeds was 49.1 ± 2.7 mg per kg. It dropped by 41.4, 32.0 and 46.2% after the application of the above-mentioned technologies. The content of indigestible oligosaccharides causing flatulence was less affected by the treatments. The pre-treatment content of raffinose was 9.5 ± 0.5 g/kg. The drop associated with the treatment was 9.5, 6.3 and 10.5%, respectively. The pre-treatment content of stachyose was 21.4 ± 0.8 g/kg and after treatment with technologies V-0 and V-II it dropped by 7.0% and by 16.4%, respectively. The application of technology V-I did not result in a drop in the content of stachyose. The content of verbascose in native pea seeds was 16.1 g/kgand the treatment with technologies V-0; V-I and V-II resulted in a drop by 7.5, 5.6 and 20.5%, respectively. As for the detected phenolic acids, with the exception of caffeic acid, not a drop, but an increase in their content was recorded. Isoflavone oestrogens such as daidzein and genistein also recorded a small increase in their content. The results of the trial lead us to conclude that the above-described methods of pea seed treatment, especially the V-II variant, proved to be useful and can be recommended for practical use.  


Weed Science ◽  
1976 ◽  
Vol 24 (2) ◽  
pp. 233-234 ◽  
Author(s):  
Abdollah Bassiri ◽  
Iraj Rouhani

Seeds of wild safflower (Carthamus oxyacanthaBieb.) collected from the Bajgah area, Iran, in 2 successive years were subjected to stratification and scarification treatments and their germination percentage and subsequent seedling growth (length) were measured at room temperature (23 ± 2 C). Maximum germination and seedling length occurred in the seeds stratified in moist sand at 4 C for 2 weeks. The response of the freshly-harvested seed to scarification was linear while that for 1-yr-old seed was quadratic. Both scarification and stratification independently increased germination but germination never reached the viability level of the seed.


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