scholarly journals Effectiveness of Ethanol Extract of Chromolaena Odorata L. Leaves as Antibacterial of Salmonella typhi and Staphylococcus aureus

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Fadia Fadia ◽  
Nurlailah Nurlailah ◽  
Tini Elyn Helmiah
Keyword(s):  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Salmonella Typhi ◽  
Inhibitory Effect ◽  
Antibacterial Drug ◽  
Chromolaena Odorata ◽  
Research Material ◽  
And Staphylococcus Aureus

Salmonella typhi and Staphylococcus aureus are pathogenic bacteria that cause infectious diseases. Treatment of these diseases can be done by giving antibacterial drugs. One of the plants that can be used as an antibacterial drug is the leaves of Chromolaena odorata L. due to the presence of chemical compounds such as flavonoids, tannins, and saponins that have the potential as antibacterial. This study aims to determine the Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) ethanol extract of Chromolaena odorata L. leaves on the growth of Salmonella typhi and Staphylococcus aureus. The study consisted of 5 treatments, namely concentrations of 20%, 40%, 60%, 80%, and 100% ethanol extract of Chromolaena odorata L. leaves with five repetitions. The antibacterial activity test was carried out by MIC and MBC methods. The research material uses leaves from Chromolaena odorata L. in the Sungai Besar area, Banjarbaru, South Kalimantan, Indonesia. The results showed that the average MIC yield of ethanol extract of Chromolaena odorata L. leaves against Salmonella typhi: 20% and Staphylococcus aureus: 20%. While the average yield of MBC values ​​against Salmonella typhi: 40% and Staphylococcus aureus: 40%. Based on the results obtained, it can be concluded that the ethanol extract of Chromolaena odorata L. leaves has an inhibitory effect on the growth of Salmonella typhi and Staphylococcus aureus.

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN KIRINYUH (CHROMOLAENA ODORATA L) SEBAGAI ANTIBAKTERI SALMONELLA TYPHI DAN STAPHYLOCOCCUS AUREUS

2020 ◽  
Vol 2 (3) ◽  
pp. 158-168
Author(s):  
Fadia Fadia ◽  
Nurlailah Nurlailah ◽  
Tini Elyn Helmiah ◽  
Leka Lutpiatina
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Herbal Medicines ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Antibacterial Drug ◽  
Chromolaena Odorata ◽  
And Staphylococcus Aureus

        Salmonella typhi and Staphylococcus aureus are pathogenic bacteria that cause infectious diseases. Treatment of these diseases can be done by giving antibacterial drugs. One of the plants that can be used as an antibacterial drug is the leaves of Chromolaena odorata L. due to the presence of chemical compounds such as flavonoids, tannins, and saponins that have the potential as antibacterial. This study aims to determine the Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) ethanol extract of Chromolaena odorata L. leaves on the growth of Salmonella typhi and Staphylococcus aureus. This research is the initial stage of the development of herbal medicines to treat Salmonella typhi and Staphylococcus aureus infections. The study consisted of 5 treatments, namely concentrations of 20%, 40%, 60%, 80%, and 100% ethanol extract of Chromolaena odorata L. leaves with five repetitions. The antibacterial activity test was carried out by MIC and MBC methods. The research material uses leaves from Chromolaena odorata L. in the Sungai Besar area, Banjarbaru, South Kalimantan, Indonesia. The results showed that the average MIC yield of ethanol extract of Chromolaena odorata L. leaves against Salmonella typhi: 20% and Staphylococcus aureus: 20%. While the average yield of MBC values ​​against Salmonella typhi: 40% and Staphylococcus aureus: 40%. Based on the results obtained, it can be concluded that the ethanol extract of Chromolaena odorata L. leaves has an inhibitory effect on the growth of Salmonella typhi and Staphylococcus aureus. Chromolaena odorata L. leaves has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

scholarly journals Preliminary phytochemical screening and susceptibility of bacteria pathogens to whole extract of evolvulus alsinoides (L.)

1970 ◽  
Vol 18 ◽  
pp. 16-20
Author(s):  
BA Omogbai ◽  
FA Eze
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Salmonella Typhi ◽  
Phytochemical Screening ◽  
Evolvulus Alsinoides ◽  
And Staphylococcus Aureus

Context: Plant based antimicrobial represent a vast untapped source for medicines and further exploration of plant antimicrobial neeto occur. Evolvulus alsinoides (L) (Convolvulaceae) is a perennial herb is used in traditional medicine in East Asia, India, Africa and Philippines to cure fever, cough, cold, venereal diseases, azoospermia, adenitis and dementia.   Objective: The objective of this research was to evaluate the antimicrobial activity of the extracts of E. alsinoides on some clinical microbial isolates.   Materials and Methods: The ed thanolic and aqueous extracts of the whole plant (leaves and twigs) were analysed for alkanoids, tannins, glycosides, steroids, flavonoids, saponins, volatile oil and resins. The determination of antibacterial activity was done using the agar well diffusion technique. Pure cultures of pathogenic bacteria such as Bacillus cereus, Staphylococcus aureus, Micrococcus leutus, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi were used for antibacterial activity assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).   Results: The ethanolic extract of the plant had MIC values ranging from 16 mg/ml to 512.5 mg/ml. The least MIC was 16mg-ml against Salmonella typhi while Bacillus cereus and Staphylococcus aureus showed the highest MIC of 512.5 mg-ml. In the aqueous extract the MIC ranged between 512.5 to >1025 mg/ml. Salmonella typhi, Micrococcus luteus and Staphylococcus aureus were not inhibited by the water extract. Phytochemical result showed ethanol to be a better solvent for the extraction of the bioactive agents in this plant which include: glycosides, alkaloids, saponins, tannins, flavonoids and volatile oil.   Conclusion: In this study the gram-negative organisms had the lowest MICs and MBCs. This suggests their higher susceptibility to the extract of this plant. On the basis of the result obtained in this investigation it can be concluded that ethanol extract of Evolvulus alsinoides had significant in vitro broad spectrum antimicrobial activity.   Keywords: Evolvulus alsinoides; Phytochemical screening; Antibacterial activity. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8769 JBS 2010; 18(0): 16-20

scholarly journals Uji Bioaktivitas Ekstrak Etanol Alga Merah Galaxaura oblongata (Ellis dan Solonder) Lamouroux. Terhadap Beberapa Jenis Bakteri Patogen. (Bioactivity Test of Red Algae Galaxaura oblongata (Ellis and Solonder) Lamouroux Ethanol Extract Against Several Types of Pathogenic Bacteria

2019 ◽  
Vol 9 (2) ◽  
pp. 67
Author(s):  
Teresia Panden ◽  
Johanis Julian Pelealu ◽  
Marina Singkoh
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Pathogenic Bacteria ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Test Results ◽  
Methicillin Resistant

Uji Bioaktivitas Ekstrak Etanol Alga Merah Galaxaura oblongata (Ellis dan Solonder) Lamouroux. Terhadap Beberapa Jenis Bakteri Patogen.(Bioactivity Test of Red Algae Galaxaura oblongata (Ellis and Solonder) Lamouroux Ethanol Extract Against Several Types of Pathogenic Bacteria) Teresia Panden1*), Johanis Julian Pelealu1), Marina Flora Oktovine Singkoh1)1)Program Studi Biologi FMIPA Unsrat Manado*Email: [email protected] Diterima  5 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 ABSTRAK Penelitian ini bertujuan menghitung nilai konsentrasi Minimum Inhibitory Concentration dan Minimum Bactericidal Concentration ekstrak etanol Galaxaura oblongata terhadap bakteri Staphylococcus aureus, MRSA (methicillin-resistant Staphylococcus aureus), dan Salmonella typhi dalam memberikan pengaruh terhadap pertumbuhan bakteri Staphylococcus aureus MRSA (Methicillin-Resistant Staphylococcus aureus), dan  Salmonella typhi. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol. Pengujian bioaktivitas antibakteri menggunakan metode dilusi cair. Hasil uji bioaktivitas antibakteri dianalisa menggunakan metode Oneway Anova, dilanjutkan dengan Uji Tukey. Uji bioaktivitas antibakteri menunjukkan ekstrak memiliki aktivitas antibakteri. Pada bakteri Staphylococcus aureus konsentrasi ekstrak 90% ditetapkan sebagai nilai MIC, MRSA pada konsentrasi ekstrak 30% ditetapkan sebagai nilai MIC, dan pada Salmonella typhi pada konsentrasi ekstrak 30% ditetapkan sebagai nilai MIC. Data Anova menunjukkan bahwa pertumbuhan koloni tiap konsentrasi ekstrak signifikan artinya tiap konsentrasi berbeda nyata dalam menghambat pertumbuhan bakteri, tetapi nilai MBC belum dapat ditentukan karena masih mengalami pertumbuhan koloni pada hasil pengujian MBC.Kata kunci : Galaxaura oblongata, bioaktivitas, senyawa kimia, antibakteri. ABSTRACT This study aimed to determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of Galaxaura oblongata ethanol extract against Staphylococcus aureus, MRSA (methicillin-resistant Staphylococcus aureus), and Salmonella typhi in influencing the growth of Staphylococcus aureus MRSA (methicillin-resistant Staphylococcus aureus), and Salmonella typhi. Extraction was carried out by maceration using ethanol solvent. The liquid dilution method was used as an antibacterial bioactivity testing. The antibacterial bioactivity test results showed that the extract had antibacterial activity. In Staphylococcus aureus bacteria, concentrations extract of 90% were determined as MIC values, MRSA of concentrations extract of 30 were determined as MIC values, and in Salmonella typhi at concentrations extract of 30% set as MIC values. The antibacterial bioactivity test results were analyzed using One Way Anova method, followed by the Tukey Test. Anova's data showed that the growth of colonies in each concentration of extract was significant, meaning that each concentration was significantly different in inhibiting bacterial growth. However, the MBC value could not be determined because it still experienced colony growth in the results of MBC testing.Keywords: Galaxaura oblongata, bioactivity, chemical compound, antibacterial.

scholarly journals Antimicrobial Efficacy and Spectrum of Phosphorous-Fluorine Co-Doped TiO2 Nanoparticles on the Foodborne Pathogenic Bacteria Campylobacter jejuni, Salmonella Typhimurium, Enterohaemorrhagic E. coli, Yersinia enterocolitica, Shewanella putrefaciens, Listeria monocytogenes and Staphylococcus aureus

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1786
Author(s):  
György Schneider ◽  
Bettina Schweitzer ◽  
Anita Steinbach ◽  
Botond Zsombor Pertics ◽  
Alysia Cox ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Campylobacter Jejuni ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Tio2 Nps ◽  
E Coli ◽  
Foodborne Pathogenic Bacteria ◽  
And Staphylococcus Aureus ◽  
Co Doped

Contamination of meats and meat products with foodborne pathogenic bacteria raises serious safety issues in the food industry. The antibacterial activities of phosphorous-fluorine co-doped TiO2 nanoparticles (PF-TiO2) were investigated against seven foodborne pathogenic bacteria: Campylobacter jejuni, Salmonella Typhimurium, Enterohaemorrhagic E. coli, Yersinia enterocolitica, Shewanella putrefaciens, Listeria monocytogenes and Staphylococcus aureus. PF-TiO2 NPs were synthesized hydrothermally at 250 °C for 1, 3, 6 or 12 h, and then tested at three different concentrations (500 μg/mL, 100 μg/mL, 20 μg/mL) for the inactivation of foodborne bacteria under UVA irradiation, daylight exposure or dark conditions. The antibacterial efficacies were compared after 30 min of exposure to light. Distinct differences in the antibacterial activities of the PF-TiO2 NPs, and the susceptibilities of tested foodborne pathogenic bacterium species were found. PF-TiO2/3 h and PF-TiO2/6 h showed the highest antibacterial activity by decreasing the living bacterial cell number from ~106 by ~5 log (L. monocytogenes), ~4 log (EHEC), ~3 log (Y. enterolcolitca, S. putrefaciens) and ~2.5 log (S. aureus), along with complete eradication of C. jejuni and S. Typhimurium. Efficacy of PF-TiO2/1 h and PF-TiO2/12 h NPs was lower, typically causing a ~2–4 log decrease in colony forming units depending on the tested bacterium while the effect of PF-TiO2/0 h was comparable to P25 TiO2, a commercial TiO2 with high photocatalytic activity. Our results show that PF-co-doping of TiO2 NPs enhanced the antibacterial action against foodborne pathogenic bacteria and are potential candidates for use in the food industry as active surface components, potentially contributing to the production of meats that are safe for consumption.

scholarly journals Identification and antibiogram study of bacterial species isolated from milk samples of different locations in Bangladesh

2016 ◽  
Vol 1 (3) ◽  
pp. 457-462 ◽  
Author(s):  
Md Nuruzzaman Munsi ◽  
Nathu Ram Sarker ◽  
Razia Khatun ◽  
Mohammed Khorshed Alam
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Public Health Importance ◽  
Milk Samples ◽  
First Choice

Cow’s milk containing pathogenic bacteria is an important threat to the consumers. The objectives of the present study were to identify the bacterial agents of public health importance in milk samples (n=35) of different locations and to determine their sensitivity to different antibiotics. The milk samples were collected and transported aseptically and subsequently allowed for culture in bacteriological media, Gram’s staining and biochemical tests for the identification of bacterial species. The bacteria identified were Staphylococcus aureus, Escherichia coli and Salmonella typhi, and their prevalence, in case of vendor milk specimens (n=28), were 96.43%, 53.57% and 35.71% respectively, and of brand milk specimens (n=7), were 42.86 %, 28.57% and 0%, respectively. This suggests that cautionary measures should be taken for quality milk production and consumption. The antibiotic sensitivity test was done by disc diffusion method and the average inhibition zones, in case of Staphylococcus aureus, were 32 mm for oxytetracycline, 26 mm for amoxicillin, 35 mm for ciprofloxacin, 27 mm for cefotaxime, 30 mm for ceftriaxone, 30 mm for azithromycin, and 26 mm for erythromycin; in case of Escherichia coli, were 5 mm for oxytetracycline, 9 mm for amoxicillin, 22 mm for ciprofloxacin, 30 mm for cefotaxime, 31 mm for ceftriaxone, 15 mm for azithromycin, and 0 mm for erythromycin; in case of Salmonella typhi., were 25 mm for oxytetracycline, 24 mm for amoxicillin, 38 mm for ciprofloxacin, 31 mm for cefotaxime, 34 mm for ceftriaxone, 24 mm for azithromycin, and 0 mm for erythromycin. Therefore, ciprofloxacin and ceftriaxone may be the antibiotics of first choice, and cefotaxime and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.Asian J. Med. Biol. Res. December 2015, 1(3): 457-462

scholarly journals Anthill Inhibiting Bacteria, a Promising Source of Bio Efficacy

2020 ◽  
pp. 14-21
Author(s):  
A. A. Katun ◽  
A. R. Abdulmumin ◽  
M. U. Yahaya ◽  
N. K. Habeeb ◽  
A. Bala
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Typhi ◽  
Biologically Active ◽  
Bacterial Strains ◽  
Active Metabolites ◽  
Bacillus Lentus ◽  
Total Prevalence ◽  
Promising Source ◽  
And Staphylococcus Aureus

The investigation into soil bacteria has been widely studied and becoming increasingly appreciated as an exceptional reservoir of unique naturally occurring biologically active metabolites with pharmaceutical applications. This article aimed to isolate, identify and biochemically characterize antibiotic-producing bacteria from anthill soils in the permanent site of Ibrahim Badamasi Babangida University, Lapai (IBBUL), Niger State, Nigeria. The sum of ten samples were collected from five sampling sites, the sampling was done in threefold (morning, noon and evening) and analyzed adopting standard microbiological protocols. The obtained result revealed that the total bacteria count in the morning ranges from 2.1×107 cfu/mL to 1.4×106 cfu/mL, noon count ranges from 3.1×107 to 2.6×106 cfu/mL while evening count was in the range of 2.1×107 cfu/mL to 1.7×106 cfu/mL. A total number of five (5) bacteria were isolated as Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Bacillus lentus and Micrococcus reseus. The total prevalence of the bacterial isolates in the morning, noon and evening were calculated as B. subtilis (109.08%), S. epidermidis (36.36%), M. reseus (36.36%), B. lentus (63.63%), and S. aureus (54.54%) respectively. These isolates were further assayed against Escherichia coli, Salmonella typhi, Klebsiella sp. and Staphylococcus aureus. The antibacterial outcome showed that two (2) (40%) anthill isolates exhibited antibacterial activity against three (3) tested bacteria (Escherichia coli, Salmonella typhi and Staphylococcus aureus). This research study has showcased that the production of inhibitory substances are common among some of the bacterial strains isolated from anthills.

scholarly journals Uji Aktivitas Antimikroba Ekstrak n-Butanol Madu Hutan (Apis nigrocincta) dari Kabupaten Selayar dengan Metode KLT-Bioautografi

2020 ◽  
Vol 1 (2) ◽  
pp. 41-45
Author(s):  
A.Suparlan Isya Syamsu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Staphylococcus Epidermidis ◽  
Salmonella Typhi ◽  
Test Results ◽  
Butanol Extract ◽  
Apis Nigrocincta ◽  
And Staphylococcus Aureus

Preliminary research has been conducted on the antimicrobial activity of n-Butanol extract of forest honey (Apis nigrocincta). This study aims to determine the antimicrobial activity of forest honey from Selayar Regency on the growth of test microbes, using the method of solid dilution with the test microbial Bacillus subtilis, Staphylococcus aureus, Streptococcus mutans, Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Vibrio sp, Staphylococcus epidermidis, and Candida albicans against n-butanol extract from forest honey (Apis nigrocincta) at 1 mg/ml. The results obtained showed that n-butanol extract inhibited the growth of bacteria Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus aureus. To estimate the compounds that provide antimicrobial activity, the TLC-Bioautography test is performed. Obtained the best results from the separation of compounds by TLC using Chlorophorom eluate: Acetone (3: 1). The TLC-Bioautographic test results showed that the spots with an Rf value of 0.29 gave activity to Bacillus subtilis, Escherichia coli, and Staphylococcus epidermidis, and gave positive results on the appearance of flavonoid compounds.

scholarly journals POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO

2015 ◽  
Vol 1 (2) ◽  
pp. 126
Author(s):  
Urnemi ◽  
Sumaryati Syukur ◽  
Endang Purwati ◽  
Sanusi Ibrahim ◽  
Jamsari
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Sds Page ◽  
Inhibition Zones ◽  
And Staphylococcus Aureus

ABSTRACT Lactic acid bacteria (LAB) were isolated from of cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii. Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, that was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12.00 mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE.  Key words: Lactic acid bacteria, Antimicrobial activity, SDS-PAGE, Cocoa fermentation and food biopreservative                                                      

scholarly journals AKTIVITAS ANTIBAKTERI JAMU MADURA SUBUR KANDUNGAN

el–Hayah ◽  
2015 ◽  
Vol 5 (2) ◽  
pp. 65
Author(s):  
Andik Wijayanto ◽  
Romi Abrori
Keyword(s):  
Centella Asiatica ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Antibacterial Drug ◽  
E Coli ◽  
Kaempferia Galanga ◽  
Test Organisms ◽  
And Staphylococcus Aureus ◽  
Paper Disc

<p><em>Madurese traditional </em><em>medicine</em><em> (herbs) Subur Kandungan is </em><em>a medicine</em><em> that is widely used</em><em> and produced</em><em> in Madura that contains Curcuma zedoaria</em><em> </em>(Chrism.)Rosc<em>., Kaempferia galanga </em>L<em>., </em><em>Foeniculum</em><em> </em><em>vulgare </em>Mill.<em>, and </em><em>Centella asiatica </em>(L.)Urb.<em> </em><em>highly favoredby the Indonesian community</em><em>, especially women to improve reproductive health but have not been tested scientifically and standardization to ensure efficacy and safety. Initial stageis screening bioactive compound and then antibacterial activity assay with paper disc method. </em><em>Clinical bacterial isolates such as Escherichia coli and Staphylococcus aureus were used as test organisms. </em><em>The best treatment to inhibit the growth of </em><em>E. Coli </em><em>and </em><em>S. aureus</em><em>is 70</em><em>% ethanol at a concentration of extract 1000 mg/ml with inhibition zone respectively 5mm and 6.5mm. Although in crude extract form, </em><em>Madurese traditional </em><em>medicine</em><em> (herbs) Subur Kandungan </em><em>had inhibitory effect on the growth of the E.coli and S.aureus so this </em><em>traditional </em><em>medicine</em><em> </em><em>potential as an antibacterial agent</em><em>. </em><em>It is recommended that further work be done to identify the specific secondary metabolite compound that responsible for this effect, purify it, and standardize as same as a antibacterial drug.</em><em></em></p>

scholarly journals PENETAPAN KADAR FENOLIK TOTAL EKSTRAK ETANOL DAUN SERUNAI (Chromolaena odorata L.) DENGAN METODE SPEKTROFOTOMETRI UV-Vis

2020 ◽  
Vol 5 (2) ◽  
pp. 54
Author(s):  
Risa Supriningrum ◽  
Henny Nurhasnawati ◽  
Siti Faisah
Keyword(s):  
Gallic Acid ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Total Phenolic ◽  
Chromolaena Odorata ◽  
Diuretic Agents ◽  
Asteraceae Family ◽  
Compound Test

Serunai (Chromolaena odorata L.) is a medicinal plant, including the Asteraceae family. Serunai is used to treat wounds, mouthwash to treat sore throats, coughs, malaria drugs, headache medications, antidiarrheals, antimicrobials, antispasmodics, antihypertensive, anti-inflammatory and diuretic agents. Serunai plants contain chemical compounds tannins, phenols, flavonoids, saponins and steroids. The purpose of this study was to determine the total phenolic content of the leaves of serunai using the UV-Vis spectrophotometric method. The stages of the research include plant determination, sampling, making of simplicia leaf of serunai, making extract by maceration method, phenolic compound test, determination of total phenolic levels by UV-Vis spectrophotometry with Folin-Ciocalteu reagent, comparing gallic acid. The results obtained by an average of total phenolic levels of ethanol extract of serunai is 171.30368 ± 1.9694 mg GAE / g means that in every gram of ethanol extract of flattened leaves is equivalent to 171,30368 mg gallic acid.

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