scholarly journals Influence of cadmium loading on morphological parameters of blood of the Laying Hens

2018 ◽  
Vol 20 (88) ◽  
pp. 48-52 ◽  
Author(s):  
A. Y. Ostapyuk ◽  
B. V. Gutyj
Keyword(s):  
Body Weight ◽  
Laying Hens ◽  
Cadmium Toxicity ◽  
The Body ◽  
Control Group ◽  
Axillary Vein ◽  
Morphological Parameters ◽  
Cadmium Sulfate ◽  
Combined Feed ◽  
Experimental Group

The article presents the results of studies on the influence of cadmium sulfate in different doses on the organism of poultry. The purpose of the work was to investigate the effect of cadmium sulfate in doses of 2 and 4 mg/kg body weight on the morphological parameters of blood of the Laying Hens. By the evaluation of the morphological analysis of the quantitative and qualitative composition of blood, it is possible to deem objectively about the functional state of the hematopoietic system of the body of the bird for cadmium loading. For research were formed three experimental groups: control group and two research groups. The control group chickens were in the usual diet, they were fed with combined feed and drank water without cadmium sulfate. To the drinking water of chickens of experimental groups added cadmium sulfate for 30 days: the first group – 2 mg/kg body weight of cadmium sulfate, the second group – 4 mg/kg body weight of cadmium sulfate. The conditions and microclimate parameters in the room for all keeping chickens groups were similar. Blood from Laying Hens was taken from the subclavian (axillary) vein in the following periods: before taking the drugs and cadmium sulfate, on the first, seventh, fourteenth, twenty-first and thirtieth day of the experiment. Hematologic studies give an opportunity to study in detail the influence of cadmium on the body of chickens, on the basis of which a more correct development of the scheme of treatment and prevention of cadmium toxicity in poultry is possible. Drinking cadmium sulfate in doses of 2 and 4 mg/kg of body weight contributed to a decrease in the number of erythrocytes to 2.69 ± 0.13 and 2.36 ± 0.14 T/l, a hemoglobin level of 86.61 ± 1.11 and 72.17 ± 0.73 g/l and an increase in the number of leukocytes to 34.9 ± 0.60 and 35.8 ± 0.66 g/l. Probable changes in morphological parameters are observed on the 21st day of the experiment in the second experimental group of Laying Hens, which were released cadmium sulfate at a dose of 4 mg/kg body weight. The hematocrit value represents the ratio between the formed elements and the blood plasma. After chickens drinking with cadmium sulfate at doses of 2 and 4 mg/kg of body weight, the hematocrit value gradually decreased. The hematocrit value was low in the second experimental group on day 21 of the experiment, where, respectively, it was 34.11 ± 0.63%.

scholarly journals Influence of milk thistle, methifene and sylimevit on the morphological parameters of laying hens in experimental chronic cadmium toxicosis

2020 ◽  
Vol 3 (1) ◽  
pp. 42-46
Author(s):  
A. Y. Ostapyuk ◽  
B. V. Gutyj
Keyword(s):  
Body Weight ◽  
Laying Hens ◽  
Modern Science ◽  
The Body ◽  
Control Group ◽  
Milk Thistle ◽  
Morphological Parameters ◽  
Treatment And Prevention ◽  
Physiological Processes ◽  
Experimental Group

One of the most pressing problems of modern science is the systematic study of natural processes, forecasting and complex assessment of changes in the environment under the influence of anthropogenic load. Blood composition is a relatively constant indicator, which is at the same time one of the labile systems of laying hens. Physiological processes that occur in the body, largely affect the quality of blood. Hematological studies make it possible to study in more detail the effect of cadmium on the body of chickens, on the basis of which a more specific development of the scheme of treatment and prevention of cadmium toxicosis in poultry is possible. The aim of this study was to investigate the effect of milk thistle, methifene and silymevitis on the morphological indices of laying hens in the development of chronic cadmium toxicosis. 32 laying hens, 78 weeks of age, were selected for the study. Four experimental groups were formed: control and three experimental. The control group (C) chickens and the three experimental groups were cadmium sulfate 4 mg/kg body weight in water. The chickens of the experimental group E1 with feed were fed the fruits of milk thistle spotted at a dose of 2.0 g/kg of feed once a day for 30 days. The chickens of the E2 experimental group were fed methifene at a dose of 0.28 g/kg of feed once a day for 30 days with feed. The chickens of the E3 experimental group were fed a sylimevit at a dose of 0.36 g/kg of feed once a day for 30 days. Drinking cadmium sulphate to laying hens at a dose of 4 mg/kg body weight contributed to a decrease in red blood cell count, hemoglobin level and an increase in leukocyte counts. With cadmium load of laying hens, milk thistle, methifene and sylimevit have a positive effect on the morphological parameters of the blood. In chronic cadmium toxicosis in laying hens, the best normalizing effect on the morphological parameters of the blood of experimental chickens is sylimevit, compared with methifene and milk thistle.

scholarly journals THE INFLUENCE OF CADMDIUM LOAD ON THE LEVEL OF VITAMINS A AND E IN BLOOD OF LAYING HENS

2020 ◽  
Vol 21 (1) ◽  
pp. 147-152
Author(s):  
A. Y. Ostapyuk ◽  
B. V. Gutyj
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Vitamin E ◽  
Vitamin A ◽  
Laying Hens ◽  
The Body ◽  
Control Group ◽  
High Biological Activity ◽  
Cadmium Sulfate ◽  
Experimental Group

Cadmium – a heavy metal, classified as a second class of danger, has a pronounced tendency to accumulate in the body. Absorbed cadmium accumulates in the liver as a complex with metallothionein. The aim of the study was to study the effect of Cadmium on the level of vitamins A and E in the blood of laying hens. For research, three groups of poultry were formed: control and two experimental. The control group chickens were on a regular diet, fed with compound feed and drinking water without cadmium sulfate. To drinking water of chickens of experimental groups for 30 days was added cadmium sulfate in doses: the first group – 2 mg/kg, the second group – 4 mg/kg of body weight. The keeping conditions of the chickens and the indoor microclimate parameters for all groups of birds were similar. After cadmium loading at a dose of 2 mg/kg body weight, the level of vitamin A in the blood of chickens has probably decreased since the 14th day of the experiment. It was the lowest at 21 days of experience. When drinking cadmium sulfate at a dose of 4 mg/kg body weight decreased levels of vitamin A by 4 and 21 days of the experiment, respectively, by 24 and 40% compared with the control group of chickens. When examining the level of vitamin E in the blood of laying hens under cadmium loading, it was found that in the second experimental group of chickens the level of this vitamin was significantly lower than in the control and the first experimental group of chickens. When compared with the control group of chickens, it was found that on the 14th day of the experiment, the level of vitamin E was lower in the first experimental group by 12% and in the second – by 27%, respectively. At day 21 of the experiment, the level of vitamin E was the lowest in the second experimental group, where it was 0.70 ± 0.011 µg/ml, respectively, which is 44% lower than the control. A significant decrease in the content of vitamins A and E indicates not only the pathological condition of the liver, but also the enhancement of oxidation processes associated with a decrease in the activity of enzymes of the antioxidant system. Cadmium compounds have high biological activity, they easily form complex compounds with proteins, nucleic acids, which inactivate a number of enzymes.

scholarly journals The effect of cadmium loading on protein synthesis function and functional state of laying hens’ liver

2019 ◽  
Vol 9 (3) ◽  
pp. 221-226
Author(s):  
B. Gutyj ◽  
A. Ostapiuk ◽  
N. Kachmar ◽  
O. Stadnytska ◽  
O. Sobolev ◽  
...  
Keyword(s):  
Body Weight ◽  
Protein Synthesis ◽  
Functional State ◽  
Total Protein ◽  
Laying Hens ◽  
Normal Diet ◽  
The Body ◽  
Control Group ◽  
Cadmium Sulfate ◽  
Experimental Group

In the article are considered issues related to the study of the effect of cadmium on the body of birds, in particular laying hens. The effect of cadmium sulfate at doses of 2.0 and 4.0 mg/kg body weight on protein synthesis function and functional state of the liver were investigated of cross-laying Hysex white hens, of 78 weeks old. Three groups of chickens were formed: two experimental and one control groups. The laying hens of the experimental groups were subjected to a cadmium load. Laying chickens of the first experimental group were given cadmium sulfate at a dose of 2.0 mg/kg body weight. Laying chickens of the second experimental group were given cadmium sulfate at a dose of 4.0 mg/kg body weight. Laying chickens in the control group were in the normal diet without cadmium. Increased activity of aminotransferases, in particular alanine and aspartate aminotransferases in their serum of hens, was observed during cadmium binge drinking in doses of 2.0 and 4.0 mg/kg body weight, respectively. These changes indicate a violation of the functional state of the liver of laying hens under conditions of cadmium load. In the study of protein synthesis of the liver of laying hens under conditions of cadmium loading, it was found that cadmium sulfate in the above doses contributed to a significant decrease in the level of total protein by 14, 21 and 30 days of the experiment. The reduction of total protein in the blood of laying hens was due to a decrease in the level of albumin, which in the second experimental group, respectively, decreased to 28.16 ± 0.75%, whereas in the first – 29.62 ± 0.98%. In the study of the level of globulins, it is possible to increase this indicator in both experimental groups of poultry for 21 days. Drinking with water cadmium sulfate at a dose of 4.0 mg/kg body weight was associated with a more likely decrease in total protein and albumin than drinking cadmium sulfate at a lower dose.

scholarly journals The Influence of the Mineral-Microbial Preparation on Ammonia Concentration and Productivity in Laying Hens Houses

Atmosphere ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 751 ◽  
Author(s):  
Remigiusz Gałęcki ◽  
Michał Dąbrowski ◽  
Tadeusz Bakuła ◽  
Kazimierz Obremski ◽  
Adriana Nowak ◽  
...  
Keyword(s):  
Body Weight ◽  
Linear Correlation ◽  
Egg Production ◽  
Laying Hens ◽  
Animal Health ◽  
The Body ◽  
Control Group ◽  
Microbial Composition ◽  
Experimental Group ◽  
Campylobacter Spp

The aim of this study was to evaluate the influence of the biopreparation Deodoric® on ammonia (NH3) concentration, performance, and hygiene standards in laying hen (ROSS-308) production. Statistically significant differences in NH3 concentration and the body weight of laying hens were observed between the control group (C) and the experimental group (E) where Deodoric® was applied at the set dose. In the control group, an increase in NH3 concentration could have contributed to the decrease in the body weight of laying hens, egg production, and % hen day egg production, whereas no such correlations were observed in the experimental group. A moderate linear correlation between NH3 concentration vs. humidity (r = 0.68), air flow (r = 0.48) and weakly linear correlation between NH3 concentration and age of birds (r = 0.27) was noted in group C. In group E, NH3 concentration vs. temperature (r = 0.27) and humidity (r = 0.14) were weakly correlated. Statistical analysis of changes in the microbial counts isolated from manure revealed a significant decrease of mesophilic microorganisms on day 28 decrease of Campylobacter spp. days 14 and 84 in group E. However, for the entire experimental model no statistically significant changes in the number of Campylobacter spp. and mesophilic bacteria were found. The tested preparation did not cause changes in the microbial composition of tissue swabs. Deodoric® contributes to animal welfare by reducing the ammonia concentrations in poultry houses. It is also recommended for use in poultry farms to improve animal health and performance and to generate benefits for producers.

scholarly journals Morphological blood indices of bulls in experimental chronic cadmium toxico-sis

2018 ◽  
Vol 20 (88) ◽  
pp. 108-114 ◽  
Author(s):  
Y. Y. Lavryshyn ◽  
B. V. Gutyj ◽  
O. R. Palyadichuk ◽  
V. Y. Vishchur
Keyword(s):  
Body Weight ◽  
Cadmium Chloride ◽  
Cadmium Toxicity ◽  
The Body ◽  
Erythrocyte Membranes ◽  
Control Group ◽  
Destructive Effect ◽  
Active Forms Of Oxygen ◽  
Young Cattle ◽  
Experimental Group

The article presents the results of research on the influence of cadmium load on the body of young cattle. The purpose of the work was to investigate morphological indices of bulls blood during the development of experimental chronic cadmium toxicity. According to the morphological analysis of the quantitative and qualitative composition of blood, it is possible to deem objectively about the functional state of the hematopoietic system of the organism of young cattle. The research was  conducted (on the basis of the farm of the village of Ivanivtsi, Zhydachiv rayon of the Lviv region) on 10 bulls of six months old, Ukrainian black-and-white milk breed, which were formed in 2 groups of 5 animals in each: control and experimental. Bulls of the control group were in the usual diet. Bulls of the experimental group were fed with a feed with cadmium chloride at a dose of 0.04 mg/kg body weight of the animal. It has been established that feeding Bulls with cadmium chloride in a dose of 0.04 mg/kg of body weight contributed to a decrease in hemoglobin to 84.5 ± 1.60 g/l and an increase in the content of methemoglobin to 5.2 ± 0.099%. The decrease in hemoglobin levels is due to the toxic effect of cadmium on erythrocytes, resulting in damage to erythrocyte membranes and increased content of methemoglobin. Formation of methemoglobin in bull blood during cadmium loading occurs as a result of oxidation of hemoglobin with the formation of superoxide anion, which in turn triggers (begins) free radical processes and exerts a destructive effect on cell membranes, as well as initiates the appearance of other active forms of oxygen. Feeding calcium cadmium chloride in a dose of 0.04 mg/kg contributed to a decrease in the number of erythrocytes in their blood. At day 20 of the experiment, the number of erythrocytes decreased by 14% relative to the control group. Decreasing the number of erythrocytes in the blood of young cattle may have been due to the inhibition of their production in the bone marrow. An important morphological indicator of blood is the determination of the average content of hemoglobin in one erythrocyte, which indicates the saturation of erythrocyte with hemoglobin. It was found that the average hemoglobin content in one erythrocyte of the bulls of the experimental group increased by 11.4 and 13.9% for 5 and 10 days. According to the results of the research, the reduction of the hematocrit values in the experimental group of bulls was detected at 10, 15 and 20 days of the experiment. A significant increase in the number of leukocytes in the blood of the bulls of the experimental group was observed at 15 days of the experiment, according to the control group indicators, it increased by 12%.

scholarly journals Protein synthesize function of bulls liver at experimental chronic cadmium toxicity

2019 ◽  
Vol 21 (94) ◽  
pp. 92-96 ◽  
Author(s):  
Y. Y. Lavryshyn ◽  
B. V. Gutyj
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Cadmium Chloride ◽  
Cadmium Toxicity ◽  
The Body ◽  
Control Group ◽  
Black And White ◽  
Wide Range ◽  
Inflammatory Processes ◽  
Experimental Group

The article presents the results of studies on the influence of cadmium load on the body of young cattle. The adverse effect of Cadmium on the liver causes a wide range of pathological changes at various levels of its organization. The purpose of the work was to investigate protein synthesize function on the liver of bulls for experimental chronic cadmium toxicity. The research was carried out on the basis of a farm at the village Ivanivtsi, Zhydachiv district of Lviv region, on 10 bulls of six months old, Ukrainian black-and-white milk breed, which were formed in 2 groups of 5 animals in each: control and experimental. Bulls of the control group were in the usual diet. Bulls of the experimental group were fed with forage with cadmium chloride at a dose of 0.04 mg/kg body weight of the animal. The research lasted for 30 days. Protein synthesize function of the liver was determined by the level of serum total protein and protein fractions. It was established that when feeding cows with feed of cadmium chloride in a dose of 0.04 mg/kg of body weight of an animal for 30 days in animals suppressed protein synthesize function of the liver, which manifests itself by lowering the total protein of blood. It reached the lowest level at the 20th day of the experiment, where, accordingly, it fluctuated within the limits of 61.9 ± 1.14 g/l. Reducing the level of total protein in the blood of the bulls of the experimental group under cadmium loading was due to lowering the level of albumin. At day 20 of the experiment, the level of albumin in the blood of animals in the experimental group decreased by 16.5%. On the background of general hypoproteinemia there is a significant imbalance between albumins and globulins in serum of diseased animals. It was established that the level of globulins increased in the blood of Bulls, which were under cadmium load. Increasing the level of globulins in blood serum of bulls reflects the intensity of inflammatory processes in their body for cadmium loading.

Effect of Clomiphene Citrate and Letrozole on Body Weight of female Albino Rat for Consecutive 1-4 Estrous Cycles

2021 ◽  
Vol 15 (11) ◽  
pp. 2938-2941
Author(s):  
Fauzia Qureshi ◽  
Syeda Rizwana Jafri ◽  
Hafiza Sadia Ahmad ◽  
Uzma Waseem ◽  
Ursula Akif ◽  
...  
Keyword(s):  
Body Weight ◽  
Estrous Cycle ◽  
Ovulation Induction ◽  
Clomiphene Citrate ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Albino Rat ◽  
Estrous Cycles ◽  
Experimental Group

Background: Ovulation induction with clomiphene citrate in women with infertility has been practiced more than 40% years but in infertile patients this treatment plan proved to be ineffective with multiple complication. Body weight plays an important role modulating reproductive development and functioning. Aim: To observe the effects on body weight of female albino rat after use of clomiphene citrate and letrozole for consecutive 1-4 estrous cycles Method: Eighty four adult female Albino rats were equally divided into three groups for this research. Body weight of each rat was measured before and after the experiment. Vaginal smear cytology of each rat was performed to study different phases of estrous cycle. Control group A was given normal saline orally , In Experimental group B rats were given letrozole (Femara) at dose 5mg/kg orally and in Experimental group C rats were given clomiphene citrate at dose 100ug/kg orally. Results: Significant weight gain is observed in rats taking clomiphene citrate as compared to letrozole Conclusion : Comiphene citrate directly affects the body weight which indirectly reduces the ovulation induction and pregnancy rate. Letrozole is good alternate for ovulation induction and for CC resistant patients. Keywords: Estrous cycle, body weight, citrate and letrozole

DYNAMICS OF PROTEIN METABOLISM AND ACTIVITY OF AMINOTRANSFERASES OF DOGS BY ADDING DIHYDROQUERCETIN

2016 ◽  
Vol 1 (1) ◽  
pp. 6-8
Author(s):  
Полищук ◽  
Sergey Polishchuk ◽  
Молянова ◽  
Galina Molyanova
Keyword(s):  
Body Weight ◽  
The Body ◽  
Control Group ◽  
German Shepherd ◽  
Formalin Edema ◽  
Working Dogs ◽  
Healthy Dogs ◽  
Biochemical Indices ◽  
Experimental Group ◽  
Operational Capacity

The purpose of research is to improve business and operational capacity of the body through the use of dogs dihydroquercetin. The article presents the results of experiment by the effect of dihydroquercetin dynamics of biochemical indices of the dogs blood. Dihydroquercetin – it is an active antioxidant, natural scavenger of oxygen free radicals, hepatoprotector, having anti-inflammatory action due to limitations of the formalin edema and histamine, and inhibits the formation of serous fluid, painkillers, immunocorrelation properties. Due to the high complexing properties it displays the body of heavy metals, including radionuclides, helps to restore the blood vessels tone, normalizing the lipid levels and slows the progression of atherosclerotic plaques. Investigations were carried out in zonal center of the dog expert service GU MVD of Russia in Samara region with clinically healthy dogs, German shepherd breed, age 2-4 years with an average body weight of 30 kg in the background conditions and feeding, adopted by the enterprise. The dogs of the experimental group received dose dihydroquercetin 0.001 g/kg for body weight once a day during meals. When added to the basic diet, dihydroquercetined dogs of the experimental group shown the increase in indicators such as: total protein – by 11.5% (p<0.01), albumin – to 12.8% (p<0.01), AST 13.6% (p <0.001), ALT – by 11% (p<0.05), alkaline phosphatase in the – 12% (p<0.01) relative to the control group. Based on the results, obtained during the experiment, we can conclude that the use of dietary supplements in the diet of dogs, dihydroquercetin allows biocorrection level of protein and enzymatic metabolism in the body, which improves service and working dogs potential.

scholarly journals Influence of the probiotic preparation “Plantarum” in the diets of pregnant animals on the growth and development of young animals

2021 ◽  
Vol 37 ◽  
pp. 00143
Author(s):  
Irina Funk ◽  
Nikolay Vladimirov ◽  
Alexander Yashkin ◽  
Lyudmila Pautova ◽  
Vitaly Gorshkov
Keyword(s):  
Body Weight ◽  
Growth And Development ◽  
The Body ◽  
Control Group ◽  
Standard Diet ◽  
Absolute Increase ◽  
Probiotic Preparation ◽  
Positive Effect ◽  
Experimental Group ◽  
Different Doses

The work aims to study the effect of different doses of the experimental probiotic preparation “Plantarum” when fed to pregnant animals on their reproductive qualities, as well as on the growth and development of young animals. To obtain young animals, four groups of goats were formed in the type of the Saanen breed, 20 heads each. In the first (control) group, the animals received a standard diet, in the diet of the animals of the second, third and fourth groups in the second half of pregnancy, the probiotic preparation “Plantarum” was additionally introduced, containing Lactobacillus Plantarum, Propiobacterium freudenreihii, in dosages from 0.4 to 0.8 ml/kg of body weight per day. From the offspring of the goats of the experimental groups, four groups of 12 goats were formed. The highest percentage of preservation (94%) and the highest business output of kids per 100 queens (150%) were observed in the fourth experimental group (0.8 ml). The goats of the second, third and fourth groups exceeded the body weight of their contemporaries from the control group by 3%, 6.3%, and 8.8%. The highest indices of the absolute increase in body weight by age periods were noted in the fourth group of goats. There were no significant differences in body build indices during the experiment between the goats of the control and experimental groups. Thus, the maximum positive effect was observed with the introduction of a probiotic preparation into the diet of pregnant goats at a dose of 0.8 ml/kg of body weight per day.

scholarly journals «Energosil» in the diets of replacement gilts

2019 ◽  
Vol 20 (2) ◽  
pp. 153-162
Author(s):  
A. A. Suvorov ◽  
в Sh. Gayirbegov ◽  
A. S. Fedin ◽  
A. M. Guryanov
Keyword(s):  
Body Weight ◽  
Live Weight ◽  
Feed Additives ◽  
The Body ◽  
Feed Additive ◽  
Control Group ◽  
Practical Training ◽  
Optimal Dosage ◽  
Growth Energy ◽  
Experimental Group

The article gives scientific substantiation of using silicon-containing new feed additive “Energosil” for replacement gilts. The studies were conducted in pig-breeding complex of the Center for Practical Training of Agricultural Specialists in Mordovia Republic. There were three experimental groups of replacement gilts (10 animals each). The effect of various dosages of Energosil (5.0, 7.5, and 10 mg per kg of body weight) on digestibility, nutrient utilization, and animal growth energy was studied. The most optimal dosage of a new feed additive in their rations was revealed, which constituted 5 mg / kg of the animal live weight. It was established that the animals from the first experimental group, who received silicon-containing feed additives of this amount digested the nutrients of the diet better than their counterparts from the other groups. Compared to the control group, the digestibility of dry matter increased by 3.62% (p<0.05), organic matter by 3.29% (p<0.01), crude protein by 4.09% (p<0.001), fat by 4.30% (p<0.01), cellulose by 3.79% (p<0.01) and nitrogen-free extractives by 2.03% (p>0.05).Replacement gilts of the first experimental group, who were given Energosil in the amount of 5 mg / kg body weight in addition to the basic ration, retained nitrogen in their bodies by 17.1%, calcium by 55.9% and phosphorus by 69.7% more than their counterparts from the control group. It contributed to an increase in body weight of gilts from the first group compared to control counterparts by 10.8%. Increased dosages of Energosil (7.5 and 10 mg / kg body weight) also had a better effect on the growth energy of gilts compared with the control group. The feed additive in the amount of 7.5 mg kg of animal  live weight contributed to an increase in animal weight compared with control counterparts by 6.21 kg or 4.8% (p<0.05), with a third experimental group by 4.4 kg or by 3.4% (p<0.05). When given Energosil in the dosage of 10 mg/kg, the body weight of gilts from the third experimental group was higher by 1.81 kg or 1.4% (p> 0.05) compared with the control counterparts.

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