scholarly journals The effect of different doses of probiotic feed additives on hematologic indices and morphological structure of individual pig internal organs

2020 ◽  
Vol 3 (1) ◽  
pp. 13-20
Author(s):  
M. Zhyla ◽  
N. Shkodyak ◽  
G. Kotsyumbas ◽  
Y. Stronskyi ◽  
O. Sobodosh ◽  
...  

In order to improve the digestibility and absorption of feed, metabolic processes, growth and development of animals, increase the resistance of the body immunomodulatory agents, probiotics, prebiotics, combined enzyme-probiotic feed additives are widely used. The purpose of our work was to conduct comparative clinical trials of the effectiveness of the probiotic feed additive Probion-forte in terms of productivity, blood indices and histological structure of individual internal  pigs organs during fattening. The researches were carried out on 120 piglets of big white breed at the age of 28 days, which were divided into 4 groups with 30 units in each one. The probiotics were added to feed in different concentrations in order to determine their efficiency and examine influence on piglets organism: the first group was given probiotic Probion-forte in dose of 1.0 g/kg (10 weeks); the second one – Probion in dose of 1.0 g/kg (6 weeks) and 0.5 g/kg (4 weeks); the third one was given probiotic Bio Plus 2B in dose of 0.4 g/kg for 10 weeks; and the fourth one was a control group. The compound feed was provided according to norms recommended for big white breed taking into account age. On the 42nd day (6 weeks) and on the 70th day of test 10 units were selected for haematological, pathomorphological and microbiological tests. The productivity of animals of all studied groups was evaluated by the average daily gains, safety, feed conversion and slaughter output. The stabilized with EDTA piglets’ blood was used for morphological studies, and blood serum – for biochemical studies. The clinical trials have shown that the application of Probion-forte, as a feed additive for fattening of piglets within 10 weeks after weaning, did not cause adverse reactions, was well tolerated by animals and contributed to the improvement of the processes of erythropoiesis and leucopoiesis, increased of serum total protein content. The increasing activity of serum transaminases indicated more intense metabolic processes in experimental animals’ organism, which was confirmed by increase in average daily weight gains and slaughter output compared to control. In the microscopic examination of the thymus, spleen, lymph nodes, intestines, liver, the characteristic histological structure of the organs was preserved and indicated their active morphofunctional state throughout the study period. Morphometrically the increase in the height of the villi in the duodenum and the size of the thymus lobes in piglets, which were fed with probiotic feed additives was established. The efficiency and appropriateness of the application of these products was confirmed in the first and second experimental groups. However, the most significant difference was observed in piglets fed with Probion-forte for 10 weeks at a dose of 1.0 g/kg of feed.

2022 ◽  
Author(s):  
Makaeva Ayna Maratovna ◽  
Sizova Elena Anatolevna ◽  
Atlanderova Ksenya Nikolaevna

Determining which forms of mineral feed additives can increase farm animal productivity is a key area of research. This study assessed the mineral composition of ruminal fluid and the effectiveness of calcium and phosphorus used by animals after the introduction of finely dispersed particles (FDP) of SiO2 (group I) and FeCo (group II) with a hydrodynamic radius of 109.6 ± 16.6 and 265 ± 25 nm, respectively. The deposition and use of calcium and phosphorus in the body of the experimental animals exceeded the control values. In group I, 30.8% more calcium was deposited (p ≥ 0.05), and in group II, the value was 30.3% (p ≥ 0.01). In the experimental groups, the calcium utilization rate was 27.3% higher in group I (p ≥ 0.05), and 28.2% higher in group II (p ≥ 0.01) compared to in the control. Phosphorus deposition was 34% higher (p ≤ 0.01) in experimental group I and 6% higher in experimental group II, compared with the control. Group I had a high utilization rate of phosphorus from the feed (with a 29% difference compared to the control). Comparison of the experimental groups revealed that the introduction of SiO2 FDP promoted an increase in the concentration of silicon, phosphorus, and calcium in the ruminal fluid. The introduction of FeCo FDP was accompanied by a decrease in the concentration of iron and cobalt in the ruminal fluid. Thus, the use of feed additive in finely dispersed form in the diet of animals was accompanied by an increase in the use of calcium and phosphorus by the animal’s body, which is advisable when intensifying milk and meat productivity. The obtained results require further research. Keywords: finely dispersed forms of microelements, ruminants, calcium and phosphorus exchange, feeding


Author(s):  
A. V. Mitfakhutdinov ◽  
E. R. Saifulmuliukov

Scientific publications describe the positive effect of feed additives and pharmacological complexes on metabolic processes in the body of broiler chickens, in particular, on the protein composition of the blood, meat productivity and protein accumulation in meat. The Peak-Antistress feed additive developed at the Department of Morphology, Physiology and Pharmacology of the South Ural State Agrarian University allows to reduce the technological load on the poultry body by stimulating the general metabolism and complex antioxidant action. Experiments on the use of the feed additive Peak-Antistress were carried out on broiler chickens of the final hybrid of the Arbor Acres cross in an industrial-type poultry farm with floor technology. The poultry was divided into three groups of 6,000 chickens each and kept in one workshop in separate sections. The control group received the main diet, the 1st experimental group received the main diet and feed additive at a dose of 1269 g / t of feed 5 days before slaughter, the 2nd experimental group received the main diet and feed additive at a dose of 1693 g / t of feed 5 days before slaughter. Slaughter of broiler chickens was carried out according to the technological instructions of the enterprise on the 38th day. Against the background of the use of the feed additive in the experimental groups of broiler chickens, stimulation of protein metabolism was observed, as evidenced by an increase in total protein in the blood by 7.9–20.1%, an increase in the level of protein in white meat by 0.1–0.3%, and a tendency to an increase in amino acids in white meat protein by 0.2–12.3%.


2019 ◽  
Vol 20 (2) ◽  
pp. 153-162
Author(s):  
A. A. Suvorov ◽  
в Sh. Gayirbegov ◽  
A. S. Fedin ◽  
A. M. Guryanov

The article gives scientific substantiation of using silicon-containing new feed additive “Energosil” for replacement gilts. The studies were conducted in pig-breeding complex of the Center for Practical Training of Agricultural Specialists in Mordovia Republic. There were three experimental groups of replacement gilts (10 animals each). The effect of various dosages of Energosil (5.0, 7.5, and 10 mg per kg of body weight) on digestibility, nutrient utilization, and animal growth energy was studied. The most optimal dosage of a new feed additive in their rations was revealed, which constituted 5 mg / kg of the animal live weight. It was established that the animals from the first experimental group, who received silicon-containing feed additives of this amount digested the nutrients of the diet better than their counterparts from the other groups. Compared to the control group, the digestibility of dry matter increased by 3.62% (p<0.05), organic matter by 3.29% (p<0.01), crude protein by 4.09% (p<0.001), fat by 4.30% (p<0.01), cellulose by 3.79% (p<0.01) and nitrogen-free extractives by 2.03% (p>0.05).Replacement gilts of the first experimental group, who were given Energosil in the amount of 5 mg / kg body weight in addition to the basic ration, retained nitrogen in their bodies by 17.1%, calcium by 55.9% and phosphorus by 69.7% more than their counterparts from the control group. It contributed to an increase in body weight of gilts from the first group compared to control counterparts by 10.8%. Increased dosages of Energosil (7.5 and 10 mg / kg body weight) also had a better effect on the growth energy of gilts compared with the control group. The feed additive in the amount of 7.5 mg kg of animal  live weight contributed to an increase in animal weight compared with control counterparts by 6.21 kg or 4.8% (p<0.05), with a third experimental group by 4.4 kg or by 3.4% (p<0.05). When given Energosil in the dosage of 10 mg/kg, the body weight of gilts from the third experimental group was higher by 1.81 kg or 1.4% (p> 0.05) compared with the control counterparts.


Author(s):  
A. R. Farkhutdinova ◽  
M. T. Sabitov ◽  
M. G. Malikova

The feasibility of using the complex mineral and vitamin feed additive “Nadezhda” in the rations of lactating first-calf heifers of Black-and-White breed has been examined in the article. It has been established that feed contain a low content of elements such as calcium, phosphorus, magnesium, sulfur, manganese, copper, zinc and cobalt. The ratio of nutrients and minerals, the degree of their availability for assimilation by the body does not provide their need. The use of the complex mineral and vitamin feed additive contributed to an increase in the milk productivity of first-calf heifers and the quality of milk, feed consumption per unit of production. All tested recipes of the complex mineral and vitamin feed additive, which have different versions of macro-, microelements and vitamins in their composition had a positive effect on the metabolic processes in the body of animals. For the production of 1 kg of milk in the experimental groups of cows less metabolic energy was spent by 5,09 (P < 0,05) and 8,06 % (P < 0,05), respectively, raw protein by 5,19 and 8,19 % (P < 0,05), digestible protein by 5,12 (P < 0,05) and 8,15 % (P < 0,05) compared with the animals of the control group. At the same time from the cows of the 1st experimental group have been received more milk by 151,6 kg in the amount of 2489 rubles, from the 2nd experimental group by 250,6 kg of milk in the amount of 4248,0 rubles. The prime cost of production of 1 kg of milk in cows of the 1st experimental group in comparison with the animals of the control group decreased by 0,72 rubles, and the 2nd experimental group by 1,21 rubles. The level of profitability of milk production in cows have been fed with additive recipe No. 1, in comparison with the animals of the control group increased by 3,71 abs.%, recipe No. 2 by 9,91 abs.%.


2021 ◽  
Vol 4 (2) ◽  
pp. 31-37
Author(s):  
O. P. Dobrianska ◽  
M. I. Zhyla ◽  
O. I. Vishchur ◽  
O. V. Deren ◽  
M. Z. Koryliak

Currently, it is important in aquaculture to study and use products and additives, which have positive effects on the digestibility of nutrients by helping to normalize the intestinal microflora, thereby modulating fish immune response. The aim of the study was to assess the effect of a prebiotic based on mannan oligosaccharides produced from the outer walls of Saccharomyces cerevisiae cells on the histological structure, morphometric state of the intestine and activity of cellular and humoral components of nonspecific resistance of age -2+ carp. The experiment was conducted in four analogous ponds, which were stocked with age-1 scaly carp with an average weight of 55–58 g with a stocking density of 1000 fish/ha. Carp had been fed balanced compound feed for 60 days. Experimental groups of fish were fed with a prebiotic as a feed additive in the amount of: 0.025 % – Experiment 1, 0.05 % – Experiment 2 and 0.075 % – Experiment 3. Use of the studied prebiotic did not damage the intestinal histological structure of carp of the experimental groups. The villi height and intestinal crypt depth of carp in Experiment 1 (P < 0.05) and Experiment 2 (P < 0.01) were larger compared to the control group. Experiment 3 showed destructive changes in intestinal mucosa. The results of histological examination indicated the effectiveness of the use of the studied prebiotic in the amount of 0.025 and 0.05 % in the diet of age -1+ carp due to the absence of pathological changes and active morphofunctional state of the intestine, which, in turn, had a positive effect on feed absorption and metabolic processes in fish body. Analysis of the indicators of cellular and humoral links of natural resistance showed that in carp of the second and third experimental groups lysozyme activity of serum was 5 and 11 % (P < 0.001) higher than in the control group. Similar changes, though found in a greater extent, were recorded in the study of bactericidal activity of blood serum, especially in individuals of the second and third experimental groups (by 6.8 (P < 0.01) and 15.2 %). While with carps of the third experimental group, which used a prebiotic drug in the amount of 0.075 %, the content of CEC was 20.5 % higher than in the control group, which indicates an additional antigenic load on the body and is an unfavorable diagnostic factor. At the same time, with the fish of the second experimental group, which received, respectively, 0.05 % of the supplement of the drug “Actigen” to the main diet, probably higher phagocytic activity of blood neutrophils was recorded. A direct dependence of the growth of the phagocytic index and the phagocytic number of neutrophils on the dose of the studied prebiotic was detected. Immunological studies indicated a dose-dependent effect of the prebiotic on the activity of cellular and humoral components of non-specific resistance of carp. Given this, for the effective functioning of innate protective mechanisms, for the increase of the body's immune potential and productivity, it is most appropriate to apply to the diet of carp prebiotic supplement “Actigen” in the amount of 0.025 and 0.05 %.


2021 ◽  
Vol 14 ◽  
pp. 59-67
Author(s):  
B.S. Ubushaev ◽  
◽  
A.K. Natyrov ◽  
B.K. Salaev ◽  
N.N. Moroz ◽  
...  

Aim. The effectiveness of the use of the mineral feed additive bischofit in the diets of young sheep in the South of Russia at various doses and its impact on the productive qualities of young sheep. Materials and Methods. The material for conducting the experiment in this household was the livestock of sheep of the Grozny breed on fattening. The sheep of the control group received a basic household diet, the I experimental group received 5 ml of bischofite per head per day in addition to the main diet, the II experimental group received 8 ml of feed additive. Classical zootechnical methods were used in the process of work, including wool yield and physical and technological properties were studied individually in 3 experimental animals from each group according to the VNIIOK method. The research materials were processed by the methods of variation statistics using the Microsoft Office software package. Results. Young sheep from the I experimental group, who received bischofite at 5 ml / head per day, were 9.4% more in live weight than in the control group. Young sheep that received 5 ml of feed additive had the best meat index, and exceeded the control group in terms of pulp yield by 12.5%. The maximum deviation from the average value of the cut of pure wool in the upward direction (110.6%) was observed in I experimental group. The most durable wool of 7.24 sN / tex is found in the group that received the bischofit feed additive as part of the main diet. Conclusion. To compensate for the lack of mineral substances of the natural and environmentally safe feed additive bischofit at a dose of 5 ml / head per day, it changes the metabolic reactions in the body in a positive way, which is confirmed by an increase in the growth of live weight and wool productivity.


Author(s):  
К.А. ГЕРЦЕВА ◽  
Д.В. ДУБОВ ◽  
А.В. КАДЫРОВ ◽  
Е.В. КИСЕЛЕВА ◽  
А.В. СИТЧИХИНА

В научной работе изучена эффективность профилактики внутренних незаразных болезней в послеродовом периоде у коров путем введения в рацион глубоко сухостойных коров кормовой добавки «Кауфит драй плюс». Эффективность кормовой добавки оценивали, анализируя частоту встречаемости незаразной патологии, данные физикального осмотра, морфологические и биохимические изменения в крови, рН-мочи и рубцовой жидкости. Исследования проводились на двух группах аналогов глубоко сухостойных коров (контрольная и опытная). Было установлено, что на 21-й день после отела в опытной группе по сравнению с контролем достоверно уменьшилась частота встречаемости: задержания последа на 11,7%, послеродового эндометрита на 10,0%, родильного пареза на 8,3%, клинического мастита на 8,4%. Однако случаи заболевания смещения сычуга за исследуемый период в опытной группе увеличились на 1,6%, кетоза – на 3,3%, гипотонии рубца – на 3,3%, асептических болезней копыт – на 4,9%. В опытной группе отмечено достоверное повышение кислотности рубцовой жидкости (с 6,82±0,38 до 5,41±0,24, р<0,01) и мочи (с 8,23±0,18 до 6,53±0,12, p<0,01); произошло достоверное снижение уровня глюкозы на 39,4% (p<0,01), увеличение содержания кетоновых тел в 2,8 раза, повышение уровня кальция и фосфора на 22,4 и 18,2% (p<0,05) соответственно. Обнаруженные изменения в биохимическом профиле крови исследуемых коров свидетельствуют о развитии энергетического дефицита в организме коров опытной группы и развитии гипокальциемии в организме коров контрольной группы. Применение кормовой добавки «Кауфит Драй плюс» достоверно снижает риск развития гипокальциемии, родильного пареза, послеродового эндометрита, задержания последа и клинического мастита, но увеличивает риск развития таких болезней в послеродовом периоде как гипотония рубца, кетоз, смещение сычуга, асептических болезней копыт. The effectiveness of prevention of internal non-contagious diseases in the postpartum period in cows due to the introduction of a feed additive "Kaufit dry plus" into the diet of deeply dry cows was studied in the scientific work. The effectiveness of the feed additive was evaluated by analyzing the incidence of non-infectious pathology, physical examination data, morphological and biochemical changes in blood, pH-urine and scar fluid. The studies were carried out on two groups of analogues of deeply dry cows (control and experimental). It was determined that on 21 days after calving in the experimental group, compared with the control, the incidence of retention of the afterbirth significantly decreased by 11.7%, postpartum endometritis by 10.0%, puerperal paresis by 8.3%, clinical mastitis by 8.4%. However, cases of abomasal displacement during the study period in the experimental group increased by 1.6%, ketosis - by 3.3%, scar hypotension - by 3.3%, aseptic hoof diseases-by 4.9%. In the experimental group, there was a significant increase in the acidity of scar fluid (from 6.82±0.38 to 5.41±0.24, p<0.01) and urine (from 8.23±0.18 to 6.53±0.12, p<0.01); there was a significant decrease in glucose by 39.4 % (p<0.01), an increase in the content of ketone bodies by 2.8 times, an increase in calcium and phosphorus by 22.4% and 18.2% (p<0.05), respectively. The detected changes in the biochemical profile of the blood of the studied cows indicate the development of energy deficiency in the body of cows of the experimental group and the development of hypocalcemia in the body of cows of the control group. The use of the feed additive "Kaufit dry plus" significantly reduces the risk of hypocalcemia, puerperal paresis, postpartum endometritis, retention of the afterbirth and clinical mastitis, but increases the risk of such diseases in the postpartum period as hypotension of the scar, ketosis, displacement of the rennet, aseptic diseases of the hooves.


Author(s):  
M. T. Sabitov ◽  
A. R. Farkhutdinova ◽  
M. G. Malikova ◽  
Yu. A. Karmatskikh

It is known that mineral additives play an important role in increasing the productivity and efficiency of the animal husbanry industry as a whole. The state of the body can be assessed by the content of mineral substances in a blood test. The purpose of the work was to study changes in morphological and biochemical blood parameters in first-calf heifers during the period of increasing the milk yield when they are fed a complex mineral and vitamin feed additive (CMVFA). The study of the influence of feeding the complex mineral and vitamin feed additive in the ration to first-calf heifers during the the period of increasing the milk yield on morphological and biochemical blood parameters has been carried out. The experiments were carried out in 2019–2020 in the OOO Breeding Farm “Valiev” in the Dyurtyulinsky district in the Republic of Bashkortostan on 3 groups of first-calf heifers of Black-and-White breed per 15 heads in each selected by the method of pairs of analogues. The animals of the control group received the main ration (MR) adopted on the farm, the 1st experimental group MR+120 g/head CMVFA according to the recipe No. 1, the 2nd experimental group MR+120 g/head CMVFA according to the recipe No. 2. In the ration of animals of the control group there was a deficiency of basic macro- and microelements. The use of CMVFA as part of the ration of first-calf heifers during the period of increasing the milk yield had a positive effect on the morphological and biochemical parameters of blood. Studies have shown that the use of CMVFA in the rations of first-calf heifers provided the need for minerals and vitamins, accelerating metabolic processes in the body during the most stressful period of lactation, the period of increasing the milk yield. According to the results of biochemical blood parameters, it is possible to esimate the improvement of protein, carbohydrate and mineral and vitamin nutrition in the first-cakf heifers of the experimental groups during the period of increasing the milk yield, which showed their genetic potential more fully and used nutrients more effectively for milk synthesis.


2016 ◽  
Vol 1 (1) ◽  
pp. 27-29 ◽  
Author(s):  
Гизатова ◽  
Natalya Gizatova

The research object is improvement of the productive qualities of heifers of Kazakh white breed by introducing additional to the basic diet of different doses of feed additives BioDarin. Protein-vitamin-mineral supplement BioDarin in its composition contains nutrients, micro- and macroelements, which are the source of energy, stimulate digestive processes, metabolism, growth and improve immunity, positive effect on animal performance. Currently of great practical and scientific importance is the use of the additive BioDarin for the correction of diet feeding. Scientific and economic experiment was carried out on the basis of JSC AIC «Alga+» Tuimazinsk district of the Republic of Bashkortostan. For the experiment there were formed 4 groups of heifers of Kazakh white-headed breed: group I – control; group II heifers in addition to the basic diet received a feed additive in a dose of 0.5 kg per 100 kg of grain mixture, the animals of group III received 1 kg of the feed additive per 100 kg of grain mixture, peers IV groups received 1.5 kg of a grain mixture. It is established that heifers of the III experienced group receiving feed additive BioDarin in the dose of 1.0 kg per 100 kg of grain mixture, had the best body weight, and average day growth.


2021 ◽  
pp. 3007-3014
Author(s):  
Sugiharto Sugiharto ◽  
Anugrah R. Pratama ◽  
Turrini Yudiarti ◽  
Tugay Ayasan

Background and Aim: In the post-antibiotic era, consumer demand for healthy and safe meats has prompted poultry producers to seek alternative effective feed additives. This study aimed to investigate the effects of a novel natural feed additive based on a mixture of Averrhoa bilimbi L. fruit filtrate, wheat bran, and Saccharomyces cerevisiae on the growth rate, internal organ weight, and breast meat characteristics of broilers. Materials and Methods: A total of 280 1-day-old chicks were divided into one control (CNTRL; feed without additives) and three treatment groups: NOV25, feed with 2.5 g/kg novel additive; NOV50, feed with 5.0 g/kg novel additive; and NOV100, feed with 10 g/kg novel additive. The body weight (BW), feed intake (FI), and feed conversion ratio (FCR) were measured weekly. On day 35, the chickens from each group were slaughtered, and their internal organs and breast meat samples were collected. Results: The BW of broilers in NOV100 was greater (p=0.016) than that in the other groups. The FCRs in the treatments groups were lower (p<0.001) than that in the control group. Elevated levels of the novel additive increased (p=0.051) the relative weight of the duodenum. The pH values in the breast meat of broilers receiving the novel additive were higher (p<0.001) than that in control. The C20:3n-6 of the NOV100 breast meat was lower (p=0.012) than that of NOV25 and NOV50, but it did not differ from that of the control. The unsaturated fatty acid-to-saturated fatty acid ratio in the breast meats of the treatments was higher (p=0.032) than that in control. The L-tyrosine content in NOV50 breast meat was higher (p=0.036) than that in CNTRL and NOV100 but did not differ from that in NOV25. Conclusion: The proposed feed additive improved the live BW and FCR of broilers and the physical and nutritional qualities of broiler breast meat.


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