scholarly journals Antifibrotics and Antioxidants of Chlorogenic Acid Inhibits Toll- Like Receptors-4 as Liver Fibrotic Marker

2021 ◽  
Vol 3 (2) ◽  
pp. 91-99
Author(s):  
Rosdiana Naibey ◽  
Widya Wasityastuti ◽  
Nungki Anggorowati ◽  
Nur Arfian

Introduction: Chlorogenic Acid (CGA) is an antifibrotic and antioxidant for fibrotic tissues. These double  roles be able to inhibit or fibrotic tissues chains because of internal and external issues.  For example, virus, bacteria or other pathogens and also by drugs, alcohol, cigarettes, etc. as external factor that affect quality of body tissues. Toll-Like Receptor-4 (TLR-4) as a marker fibrotic tissues.  It is a key for researcher could be find out by expression performance. The aim of this study is to reveal the CGA as a candidate of antifibrotic & antioxidant in liver fibrosis that induced by CCL4.    Methods: This is a pure experimental research with a simple experimental design or post-test only control group design. The total 29 mices of 2.5-month-old male Swiss mices with weigh 35-40 gram divided into 6 group: 3 groups of controls (injected by natrium chloride, CGA, and CCL4) and 3 groups of treated (injected by CGA doses 42 mg/kg, 63 mg/kg or 84 mg/kg).  Liver organ was used to examine the expression of TLR-4 by rt-PCR. This research revealed that expression of TLR-4 lower than the CCL4 control group (respectively, p=0.042; p=0.005; p=0.006; and p=0.001). Higher dose of CGA showed greater ability as anti-fibrotic through inhibit the expression of  TLR-4. Some research found the expression of TLR-4 has been decreased by treatment of Clorogenic Acid (CGA). Conclusion: To sum up, CGA has double roles to repair liver fibrotic tissues. The greater doses of CGA, the stronger inhibition of TLR-4 expression.

2012 ◽  
Vol 15 (3) ◽  
pp. 151-158
Author(s):  
Aria Wahyuni ◽  
Elly Nurrachmah ◽  
Dewi Gayatri

AbstrakPenyakit Jantung Koroner (PJK) adalah suatu bentuk gangguan pembuluh darah koroner yang termasuk dalam ketegoriarterosklerosis. Ketidaksiapan pasien PJK pulang dari rumah sakit akan berdampak terhadap rawatan ulang sebagai akibat daripelaksanaan program discharge planning yang belum efektif selama dirawat. Penelitian ini bertujuan untuk mengetahui pengaruhpenerapan discharge planning terhadap kesiapan pulang pasien penyakit jantung koroner. Penelitian ini menggunakandesain quasi experiment dengan pendekatan non-equivalent post test only control group design. Jumlah sampel 32 orang yangterbagi atas 16 orang kelompok kontrol dan 16 orang kelompok intervensi dan dilakukan di tiga rumah sakit di Kota Bukittinggi.Hasil penelitian didapatkan adanya pengaruh penerapan discharge planning terhadap kesiapan pulang pasien penyakit jantungkoroner yang terdiri dari status personal, pengetahuan, kemampuan koping, dan dukungan (p= 0,001; α= 0,05). Penelitian inimerekomendasikan discharge planning yang baik dapat dilakukan untuk meningkatkan kualitas asuhan keperawatan dan kualitashidup pasien penyakit jantung koroner.Kata Kunci: discharge planning, kesiapan pulang, penyakit jantung koronerAbstractCoronary Heart Disease (CHD) is a form of blood vessel disorder that belongs to the category of coronary atherosclerosis. Anunreadiness of patients with CHD to go home from the hospital will have an impact on readmission as a result of ineffectivedischarge planning program during hospitalized. The purpose of this study was to examine the effect of the implementation ofdischarge planning program on the readiness to be discharged from the hospital. A quasi experiment with non-equivalent posttest only control group design was employed. The participant of the study was 32 respondents devided into control andintervention groups, each had 16 respondents who were taken from three hospitals in Bukittingi. The result showed thatdischarge planning program has significance influence on patient’s perception of their readiness to be discharged from thehospital, it consisting of personal status, knowledge, coping ability, and support (p= 0.001; α= 0.05). This study recommendsthat a good discharge planning program can be implemented to improve the quality of nursing care, to reduce the risk ofreadmission to the hospital and the quality of life of patients with coronary heart diseases.Keywords: coronary heart disease, discharge planning, readiness to be discharged


2019 ◽  
Vol 9 (3) ◽  
pp. 219-224
Author(s):  
Nieniek Ritianingsih

Penyakit gagal ginjal kronis atau disingkat GGK, saat ini diakui sebagai prioritas kesehatan masyarakat di seluruh dunia. Pasien GGK akan mengalami  gangguan fisik dan psikologis sehingga mempengaruhi kualitas hidupnya. Teknik konservasi energi dengan pemberdayaan diri dapat diberikan pada pasien GGK dengan tujuan dapat meningkatkan energi, mengurangi fatigue dan kualitas hidup dapat meningkat. Penelitian ini bertujuan untuk mengetahui pengaruh penerapan teknik konservasi energi dengan pemberdayaan diri terhadap kualitas hidup pasien gagal ginjal kronik.Hipotesis penelitian ini adalah teknik konservasi energydengan pemberdayaan diridapat meningkatkan kualitas hidup pasien GGK.   Responden penelitian ini berjumlah 47 orang untuk kelompok intervensi dan untuk kelompok kontrol 47 orang responden. Penelitian ini merupakan penelitian kuantitatif menggunakan metoda kuasi eksperimental dengan pendekatan pre test – post test control group design. Hasil uji statistik didapatkan nilai p value 0,000 maka dapat disimpulkan ada perbedaan yang signifikan antara  kualitas hidup  kelompok kontrol dengankualitas hidup kelompok intervensi setelah dilakukan penerapan teknik konservasi energy. Perawat agar menerapkan tehnik konservasi energy sebagai suatu program terstruktur dari intervensi keperawatan bagi pasien GGK   Kata kunci : teknik konservasi energi, kualitas hidup, GGK   THE EFFECT OF THE APPLICATION OF ENERGY CONSERVATION TECHNIQUES WITH SELF-EMPOWERMENT TO THE QUALITY OF LIFE OF PATIENTS WITH CHRONIC RENAL FAILURE   ABSTRACT Chronic kidney failure or abbreviated CRF, is currently recognized as a public health priority throughout the world. GGK patients will experience physical and psychological disorders that affect their quality of life. Energy conservation techniques with self-empowerment can be given to CRF patients with the aim of increasing energy, reducing fatigue and quality of life can be increased. This study aims to determine the effect of the application of energy conservation techniques with self-empowerment to the quality of life of patients with chronic renal failure. The hypothesis of this study is conservation techniques of energy with empowerment can improve the quality of life of patients with CRF. The respondents of this study were 47 people for the intervention group and for the control group 47 respondents. This research is a quantitative research using a quasi-experimental method with a pre test - post test control group design approach. The statistical test results obtained p value 0,000, it can be concluded that there is a significant difference between the quality of life of the control group and the quality of life of the intervention group after the application of energy conservation techniques. The nurse is to apply energy conservation techniques as a structured program of nursing interventions for CRF patients   Keywords: energy conservation techniques, quality of life, CRF


2018 ◽  
Vol 54 (2) ◽  
pp. 84 ◽  
Author(s):  
Widayati Agustina ◽  
Widjiati Widjiati ◽  
Alfiah Hayati

This study aimed to determine the effects of red fruit (Pandanus conoideus Lam) oil on MDA levels and spermatozoa quality in mice (Mus musculus) exposed to MSG. The quality includes motility, viability, concentration, and morphology of spermatozoa. This experimental study used randomized post-test only control group design. The subjects of this study were 25 mice (Mus musculus), divided into 5 groups (5 mice per group). K- group received distilled water for 35 days. K+ group received 4 mg/g BW MSG for 21 days. P1, P2, and P3 treatment groups received 4 mg/g BW MSG for 21 days and 0.02; 0.04; 0.08 ml/g BW red fruit oil, respectively, from day 22 to 35. The results showed that mean spermatozoa morphology in K-, K+, P1, P2, P3 groups were as follows: 0.86; 0.56; 0.67; 0.61; and 0.87 (%). The spermatozoa concentrations were sequentially as follows: 21; 10; 15; 32,8,19 (107 cells/ml). The spermatozoa's vitalities were as follows: 0,64; 0,14; 0,24; P2: 0.36; 0.68 (%). MDA levels were respectively: 0.29; 0.60; 0.35; 0.23; and 0.19 (nm). As a conclusion, testicular MDA levels in mice exposed to MSG and given with red fruit oil were lower than those in mice exposed to MSG without receiving red fruit oil. The quality of spermatozoa in mice exposed to MSG and receiving red fruit oil was higher than that of mice exposed to MSG without being given with red fruit oil.


2021 ◽  
Vol 4 (2) ◽  
pp. 273-279
Author(s):  
Ida Ayu Manik Damayanti ◽  
Putu Indrayoni ◽  
Ni Wayan Sukma Antari ◽  
Anak Agung Istri Mas Padmiswari

The purpose of this study was to determine the effect of giving Averrhoa bilimbi leaf extract on sperm quality of diabetic mice. This research is a pure experimental (true experimental) with a post-test-only control group design approach. This research was conducted by giving Averrhoa bilimbi leaf extract as a treatment for 42 days in male mice. Sperm quality parameters observed included viability, abnormalities, motility in sperm. In all variables, the results of the data showing a normal distribution with a p-value > 0.05 were then carried out with a parametric test using one-way ANOVA. Averrhoa bilimbi leaf extract can increase the number of spermatogenic cells in male mice with hyperglycemia.


NSC Nursing ◽  
2021 ◽  
pp. 34-49
Author(s):  
Herinawati Herinawati ◽  
Atikah Fadhilah Danaz Nasution ◽  
Lia Artika Sari ◽  
Iksaruddin Iksaruddin

Background: The inappropriate temperature of vaccine storage may cause vaccine damage leading to degrading or even dispelling the vaccine’s quality. This research aims to reveal the difference of vaccine’s quality stored in the cooler box compared to the household refrigerator with vaccine only and the difference of vaccine’s quality stored in the cooler box compared to the household refrigerator with vaccine stored along with food and beverage Methods: The research design uses an experimental study with a post-test-only control group design. The research was conducted at two independent practice midwives in Antapani Sub-district and the Faculty of Mechanical and Aerospace Engineering Bandung Institute of Technology from November to December 2015. The research object used eight types of vaccine (Hepatitis B, BCG, DPT-HB-Hib, Polio, Measles, DT, Td, and TT) with 72 vaccine vials divided into 3 vaccine storage units. The data analysis uses the chi-square test. Results: The results of the research show that there are statistically significant differences between the vaccine’s quality stored using a cooler box compared to a household refrigerator with vaccine only and the vaccine’s quality stored using a cooler box compared to a household refrigerator with vaccine stored along with food and beverage with the value of p<0,05. Conclusion: It is concluded that there are differences of the vaccine’s quality stored using cooler box compared to a household refrigerator with vaccine only and also different vaccine’s quality stored using cooler box compared to a household refrigerator with vaccine stored along with food and beverage Keywords: Cooler box, household refrigerator, vaccine quality


2018 ◽  
Vol 10 (2) ◽  
Author(s):  
Corazon H. Dumaria ◽  
A AGP Wiraguna ◽  
Wimpie Pangkahila

Abstract: This study was aimed to prove that administration of 10% redfruit extract cream could prevent the increase of skin melanin in guinea pig exposed to UVB and to compare this effect with 4% hydroquinone cream. This was a true experimental study using post test only control group design. Subjects were devided into three groups; each of 10 guinea pigs. Group 1, the control group, was exposed to UVB and applied with basic cream. Group 2 was exposed to UVB and applied with 4% hydroquinone cream. Group 3 was exposed to UVB and applied with 10% redfruit extract cream. The total dose of UVB was 390 mJ/cm2 given for 2 weeks. The amount of melanin was calculated using the percentage of the pixel area of melanin and was compared with the pixel areas of all epidermal tissues. The results showed that the highest percentage of melanin area was in group 1 (19.78%±3.79%). The percentage of melanin area in group 3 was 1.25%±0.76% meanwhile in group 2 was 0.85%±0.37%. There were signi-ficant differences in melanin percentage between the control group and group 2 as well as group 3 (P <0.05). There was no significant difference in melanin percentage between group 2 and group 3 (P >0.05). Conclusion: The 10% redfruit extract cream could prevent the increase of skin melanin in guinea pig exposed to UVB as effectively as the 4% hydroquinone cream.Keywords: redfruit extract cream, melanin, UVBAbstrak: Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak buah merah 10% dalam mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB dan perbandingannya dengan krim hidrokuinon 4%. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Sampel terdiri dari tiga kelompok dengan jumlah sampel 10 ekor marmut jantan tiap kelompok. Kelompok 1 yaitu kelompok kontrol, diberi paparan sinar UVB dan diolesi krim dasar. Kelompok 2 diberi paparan sinar UVB dan diolesi krim hidrokuinon 4%. Kelompok 3 diberi paparan sinar UVB dan diolesi krim ekstrak buah merah 10%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin tertinggi pada kelompok 1 (19,78± 3,79%) dan terendah pada kelompok 2 (0,85±0,37%), sedangkan jumlah melanin pada kelompok 3 di antara keduanya (1,25±0,76%). Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05). Tidak terdapat perbedaan bermakna antara kelompok 2 dan 3 dalam mencegah peningkatan jumlah melanin (P >0,05 ). Simpulan: Krim ekstrak buah merah 10% dapat mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB sama efektif dengan krim hidrokuinon 4%.Kata kunci: krim ekstrak buah merah, melanin, UVB


2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Risnawati A. Haris ◽  
Lydia Tendean ◽  
Grace Turalaki

Abstract: The coffee beans composed of the compound caffeine, palmitic acid, linoleic acid and acid stearic. The content of caffeine in coffee can improve spermatozoa motility that are not motile by inhibiting cyclic nucleotide phosphodiesterase and affects the intracellular levels of cyclic AMP. Spermatozoa quality can be affected by various factors such as stress. In nocturnal animals, dark periode becomes a signal to do activity by suprachiasmatic nucleus. The change in activity of the nocturnal animals from initial condition in the dark then exposed to light can leads to stress. This study was aimed to determine the effect of coffee on the spermatozoa quality of wistar rats which had been exposed to stress. This was an experimental analytical study with a post-test only control group design. The study was conducted for 50 days from September to November 2016. The sample consisted of 9 rats divided into three groups: a control group (P0) is given only exposure to stress in the form of light, the treatment group 1 (P1) is given exposure to stress in the light form and liquid coffee 1 ml / kg and the treatment group 2 (P2) is given exposure to stress in the light form and coffee extracts. The results showed that there were significant differences in the concentration and motility treatment group 1 (P1) to the control group (P0) which is p <0.05 and significant differences in sperm motility treatment group 2 (P2) to the control group (P0) which is p <0.05. Conclusion: Coffee administration could improve the quality of spermatozoa of wistar rats exposed to stress.Keywords: stress, coffee, quality of spermatozoa. Abstrak: Biji kopi tersusun dari senyawa kafein, asam palmatik, asam linoleat dan asam stearik. Kandungan kafein yang terdapat didalam kopi mampu meningkatkan motilitas spermatozoa yang tidak motil dengan cara menghambat siklus nukleotida fosfodiesterase dan mempengaruhi level intraseluler dari siklus AMP. Kualitas spermatozoa juga dapat dipengaruhi oleh berbagai faktor lain seperti stres. Pada hewan nokturnal periode gelap menjadi sinyal untuk beraktiftas dilakukan oleh suprachiasmatic nucleus. Perubahan aktivitas pada hewan nokturnal dari kondisi awalnya ditempat yang gelap lalu diberikan paparan cahaya dapat menyebabkan terjadinya stres. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian kopi terhadap kualitas spermatozoa tikus wistar yang terpapar stres. Jenis penelitian ialah eksperimental analitik dengan post-test only control group design. Penelitian dilakukan selama 50 hari dari bulan September – November 2016. Sampel sebanyak 9 ekor tikus dibagi menjadi tiga kelompok yaitu : kelompok kontrol (P0) hanya diberikan paparan stres berupa cahaya, kelompok perlakuan 1 (P1) diberikan paparan stres berupa cahaya dan cairan kopi 1 mL/KgBb dan kelompok perlakuan 2 (P2) diberikan paparan stres berupa cahaya dan ekstrak kopi. Hasil penelitian menunjukkan adanya perbedaan yang signifikan pada konsentrasi dan motilitas spermatozoa kelompok perlakuan 1 (P1) terhadap kelompok kontrol (P0) yaitu p<0,05 dan perbedaan yang signifikan pada motilitas spermatozoa kelompok perlakuan 2 (P2) terhadap kelompok kontrol (P0) yaitu p<0,05. Simpulan: Pemberian kopi meningkatkan kualitas spermatozoa yang terpapar stres. Kata kunci: stres, kopi, kualitas spermatozoa


2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Putri Puspitasari ◽  
AAG Wiraguna ◽  
Wimpie Pangkahila

Abstract: This study was aimed to evaluate the effectiveness of 20% green tea extract in preventing the increase of melanin in guinea pig skin exposed to UV-B and to compare the effectiveness of this treatment to 4% hydroquinone cream. This was an experimental study with the post-test only control group design. Subjects were four groups; each consisted of 7 male guinea pigs. Group 1, the control group, was exposed to UV-B only. Group 2 was exposed to UV-B and applied with cream based substance. Group 3 was exposed to UV-B and applied with 4% hydroquinone cream. Group 4 was exposed to UV-B and applied with 20% green tea extract. The total UV-B intensity was 390 mJ/cm2 consistently exposed for two weeks. Histopathological slides of the skin tissue were stained with Masson Fontana technique. The total amount of melanin was calculated in percentage of pixel compared to pixel of melanin in total epidermis. The results showed that the highest amount of melanin was in group 1 (24.44%) and the lowest amount of melanin was in group 3 (1.04%) meanwhile the amount of melanin in group 4 (1.34%) was nearly the same with group 3. There were significant differences between group 1 to group 3 and 4 (P < 0.05). There was no significant difference between group 3 and group 4 (P > 0.05). Conclusion: The 20% green tea extract was as effective as 4% hydroquinone cream in inhibiting the increase of melanin in guinea pigs’skin exposed to UV-B.Keywords: green tea extract, melanin, ultraviolet BAbstrak: Penelitian ini bertujuan untuk mengetahui efek pemberian krim ekstrak teh hijau 20% terhadap peningkatan jumlah melanin pada kulit marmut yang dipajan sinar UVB serta membandingkan efektivitasnya dengan krim hidrokuinon 4%. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subyek penelitian terdiri dari empat kelompok dengan 7 ekor marmut jantan setiap kelompok. Kelompok 1 (kelompok kontrol) diberi pajanan sinar UV-B saja. Kelompok 2 diberi pajanan sinar UV-B dan diolesi krim dasar. Kelompok 3 diberi pajanan sinar UV-B dan krim hidrokuinon 4% sedangkan kelompok 4 diberi pajanan sinar UV-B dan krim ekstrak teh hijau 20%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Sediaan histopatologik jaringan kulit dipulas dengan pewarnaan Masson Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin tertinggi pada kelompok 1 (24,44%) dan terendah pada kelompok 3 (1,04%). Jumlah melanin pada kelompok 4 (1,34%) hampir sama dengan kelompok 3. Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 3 dan 4 (P < 0,05). Perbandingan antara kelompok 3 dan 4 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P > 0,05). Simpulan: Pemberian krim ekstrak teh hijau 20% sama efektif dengan krim hidrokuinon 4% dalam mencegah peningkatan jumlah melanin kulit marmut yang dipajan sinar UV-B.Kata kunci: krim ekstrak teh hijau, melanin, ultraviolet B


Author(s):  
Ni Putu Ariesta Budiani ◽  
Nyoman Adiputra ◽  
I Wayan Surata

American-style service (American Service) priority to speed and accuracy amongwaitress while did the ministry. Therefore a waitress required to be able to carry a maximum of four plates at a time when doing service. The application of inappropriate tableware may associate with musculoskeletal complaints and fatigue, and affect the service performance in the restaurant. It is necessary for the application of ergonomic aspect especially appropriate equipment.This research was using an experimental study with the pre and post test control group design. This study involved fourth semester students, Restaurant Management Third Diploma of Nusa Dua Bali Tourism Institute as a subject as many as 36 people. The result showed significantly difference between control and treatment groups. In the treatment group  had  decreased  subjective  complaints  that include  musculoskeletal disorders about 38.7 % and general fatigue about 42.9 %. The length of presentation time decline 36.86%, while the quality of the presentation increased 65.01%. The decrease insubjective complaints occurred because the application of anthropometric dinner plate thatlighter 410 grams and has a shorter diameter 4.2 cm than previous plate. It is more convenient to carry even four at the time when presents to the guests. This is also showed an increase inservice performance. It can be concluded that the ergonomics  application  of using  appropriate anthropometric equipment in the American Service can improve the service performance among practice students in restaurant.


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