scholarly journals Germination and seedlings growth of domestic poppy (Papaver somniferum L.) regarding salinity and temperatures

2021 ◽  
Vol 82 (4) ◽  
pp. 157-172
Author(s):  
Ivana Varga ◽  
Manda Antunović ◽  
Dario Iljkić ◽  
Milan Pospišil ◽  
Mirta Rastija

In this research there were studied the effects of NaCl-induced salinity and environment temperatures on germination and seedlings growth of domestic poppy. The study was conducted in the controlled conditions in the plant growth chamber. There were applied different concentrations of NaCl (0 mM (control), 50 mM, 100 mM and 150 mM) and environment temperatures (10°C, 15°C and 20°C). The 50 seeds of domestic poppy cultivar (Detkovac) were sown in 4 replicates on the filter paper. The germination energy was determined on the 5th day and total germination rate and seedlings morphological characteristic were determined on the 10th day. At the 5th day germination energy was on average 28%. It was interesting to note that at the 10°C there were no germinated seeds after 5 days at all salinity treatments and also, at the salinity level of 150 mM NaCl at every temperature. The average germination rate (on 10th day) of domestic poppy seeds were 52% and it varied from 30% (150 mM NaCl and 20°C) to 90% (0 mM NaCl and 15°C). Different salinity of water solution had a very significant (p<0.01) influence on stem, root and total length (cm) of seedlings. The average root length was 1.3 cm and it varied from 0.9 cm (100 mM NaCl) to 1.8 cm (0 NaCl). The average stem length was 1.8 cm. The longest stem was found at control (2.7 cm), and between 50 and 100 mM of NaCl the difference was not significant and stem length averaged 1.4 to 1.3 cm depending on the temperature. Average total poppy seedlings length of this study was 3.0 cm and it varied from 4.5 cm at the control to 2.3 cm at 100 mM NaCl. With regard to temperatures, there was no significant differences found in root and total length of poppy seedlings, but the differences were very significant (p<0.01) for stem length. The longest poppy seedlings were measured at 20°C and 0 mM NaCl (4.9 cm), while at 10°C and at 0 or 50 mM NaCl, seedlings were less than 0.1 cm. Seeds were not germinating at all on 10°C on both, 100 and 150 mM NaCl. Generally, salinity reduced germination energy and germination rate and seedlings length. Results may indicate that seeds are mainly affected by osmotic stress and therefore it is not recommended to cultivate poppies on soils with excess salts

Sjemenarstvo ◽  
2021 ◽  
Vol 31 (1-2) ◽  
pp. 13-20
Author(s):  
Ivana Varga ◽  
Manda Antunović ◽  
Mirta Rastija ◽  
Dario Iljkić ◽  
Monika Tkalec Kojić

In this study the fiber flax (Linum usitassimum L.) cultivar Lirina was tested to seed germination under salinity of NaCl and two different temperatures. The experiment was set up in controlled conditions in fitotron (Aralab). There were two temperatures (10°C and 20°C) and different NaCl water solution used for salinity stress: 0 mM, 20 mM, 40 mM, 60 mM, 80 mM and 100 mM. There were 100 seeds sown in 4 replications. The germination energy (%) and germination rate (%) were determined on 3rd and 7th day, respectively. At the lower temperature (10°C) the seeds were not sprouted on 3rd day, whereas at higher temperature (20°C) mean germination energy was 31%. Germination rate (7th day) was quite similar at both temperatures (58% at 10°C and 59% at 20°C). The higher salinity stress of 80 and 100 mM had negative influence on germination energy as well germination rate. With increased salinity, the total seedlings length was also decreased. With increasing salinity (over 20 mM), the total seedlings length was also decreased. In out study, low salinity stress of 20 mM even increases the germination rate and germination energy and resulted with the longest seedlings of the fiber flax cultivar Lirina.


Sjemenarstvo ◽  
2021 ◽  
Vol 31 (1-2) ◽  
pp. 21-28
Author(s):  
Ivana Varga ◽  
Manda Antunović ◽  
Tsvetelina Dobreva ◽  
Dario Iljkić ◽  
Jasna Šoštarić

In this study the confectionery sunflower seed was tested to germination at different pH media, from 3.5 to 8.5. The experiment was set up at room temperature (22°C) and at 24 h dark conditions for 10 days. The mean germination rate was 76%. The highest germination rate was 86% at pH 3.5, whereas the lowest was 68% at pH 5.5. The ANOVA resulted with very significant influence of pH on sunflower seedlings root, stem and total length (p<0.001). The total length of seedlings was the shortest at acid media (9.2 cm at 3.5) and the longest at alkaline media (12.5 cm at pH 8.5). Regression equitations showed that increment of stem or root length and total length have a positive trendline at all pH levels. It was found that the alkaline water solution has a positive influence on protein sunflower seedlings development, but it would be valuable to found how the seeds would germinate at the field conditions.


Author(s):  
V. Turlyun

The analysis of some herds of imported cattle under the conditions of Russian farms had been shown that the genetic potential of imported cattle in many farms has being realized only by 57 %, with the output of up to 50 % of the cattle during the first 2 years. The reason for this is the discrepancy between the conditions of the biological needs of animals. In this regard, the study of factors that affect the provision of comfortable conditions for animals plays an important role. This is especially true for Holstein animals, which are more susceptible to various diseases. In accordance with the technological solutions used in modern large complexes, the loose housing method of maintenance is mainly used. This method allows the animal to move freely, providing access to the consumption of water and feed, as well as timely rest. This determines the importance of the requirements for the size of the boxes for the rest of animals, which should ensure dryness and cleanliness in the process of resting cows, prevent damage to animals, as well as the ability of other animals to displace each other. The crossbars should not interfere with the free movement of the cow in the process of lowering to the floor and getting up due to their flexibility. The dimensions of the boxes should be made taking into account the measurements of the animals’ torso, as well as the amplitude of movement in the process of lowering and rising. The purpose of the research was to study the size of boxes and their compliance with the biological needs of highly productive imported cattle. An analysis of the compliance of conditions for housing highly productive cattle of Canadian and Australian origin with their biological needs under the conditions of a mega farm has been presented in the paper. Calculation on the basis of measurements of animals has been shown that animals of the Canadian selection require boxes with a total length of at least 279 cm, Australian – 271 cm. The difference with the required width of the box has been also established. For the group of cows of Canadian selection it should be at least 120 cm, for the Australian – 114 cm. Research results have been shown that the discrepancy in the design of boxes for comfortable rest of animals is a deterrent to the realization of their genetic productive potential.


1992 ◽  
Vol 2 (1) ◽  
pp. 15 ◽  
Author(s):  
L Valbuena ◽  
R Tarrega ◽  
E Luis

The influence of high temperatures on germination of Cistus laurifolius and Cistus ladanifer seeds was analyzed. Seeds were subjected to different temperatures for different times, afterwards they were sowed in plastic petri dishes and monitored for germinated seeds over two months.The germination rate observed in Cistus ldanifer was greater than in Cistus laurifolius. In both species, heat increased germination percentages. For Cistus laurifolius higher temperatures or longer exposure times were needed. Germination percentages of Cistus ladanifer were lower when heat exposure time was 15 minutes.It must be emphasized that germination occurred when seeds were not treated, while seeds exposed to 150�C for 5 minutes or more did not germinate.


Author(s):  
Agostino Marioni ◽  
Roberto Dalpedri ◽  
Marco Banfi ◽  
Carla Cai

<p>The Padma Bridge is a multipurpose road-rail bridge across the Padma River currently under construction in Bangladesh and is one of the most important projects in the whole south east Asia with a total length of 6150 m. The maximum design vertical load reaches 98725 kN.</p><p>The Holtekamp Bridge is a steel road bridge with 2 main spans of 150 m and 2 lateral spans of 75 for a total length of 450 m. The bridge is isolated by use of sliding pendulum isolators having a maximum vertical load capacity at ULS of 54915 kN.</p><p>In both cases the isolators are double sliding surface type but with a different approach about the central articulation. The paper aims to put in evidence the difference in behaviour of the 2 types of pendulum, analysing both the theoretical approach and the test results with a special focus over the adopted solution for perform the test on a device which exceed the capacity of aby existing laboratory.</p>


1981 ◽  
Vol 21 (06) ◽  
pp. 679-686 ◽  
Author(s):  
W.H. Seitzer

Abstract In a concentric cylinder viscometer. Utah shale oils have different characteristics, both at equilibrium flow and during start-up from rest, depending on whether the wax has crystallized as needles or spherulites. Compared with waxy crude oils, which are thixotropic, shale oil had the added rheological property of being antithixotropic. Introduction The most likely liquid synthetic fuel to be produced initially in the U.S. will be raw shale oil from western oil shale. This abundant resource is located principally in the western Rocky Mountain states of Colorado. Utah. and Wyoming (Fig. 1). Ultimate commercial production probably will be transported to marketing, distribution, and refining centers by pipeline. It has been reported that Utah shale oils produced by the Union "B" and Paraho DH retorting processes gave similar physical and chemical properties. Some properties of the two Utah shale oils are given in Table 1. The only major difference is that the Union shale oil has a pour point of - 1 degree C compared with a pour point of 25 degrees C for the Paraho oil. Wax Crystallization The difference in the pour points of the oils from the Utah shale retorted by Union Oil Co of California and Paraho is caused mainly by the difference in how the wax in the respective oils crystallizes. In the high- pour-point (25 degrees C) Paraho DK oil, the wax, under a microscope, appears as fine (1 to 10 m) needles, as expected for normal paraffins. However, the wax in the low-pour-point (−1 degrees C) Union oil forms small spherulites.Wax spherulites have not been reported before: however, this type of crystal is seen commonly in polymer. Spherulites show up as round areas containing a maltese cross when observed between crossed polars under a microscope.Photomicrographs of these crystals are shown in Figs. 2 and 3. The former, showing spherulites, is of the Union oil. In contrast, they are very different from the customary needles as typified by the Paraho oil in the latter micrograph. Presumably, these highly ordered spheres are made up of wax needles grown out radially from the center as described by Hartshorne and Stuart. The polarized light is scattered only by those needles not parallel nor perpendicular to the plane of polarization. Viscometer Measurements To understand the effect of these spherulites on the flow characteristics of raw shale oil at flow conditions expected in a long-distance pipeline, typical stress-rate measurements were made in a rotating cylinder viscometer, the Haake Rotovisco RV3 with MK500 measuring head and MVI coaxial cylinder sensor having an 82-mm cup and radii ratio of 0.95. This equipment has provisions for varying shear rate continuously at selected values down to 23.4 sec(−1)/min and can produce and record shear stress as a function of either shear rate or time. Calibration of the sensor was verified with a sucrose/water solution at several temperatures.Changes in temperature always were made from lower to higher to keep the sensor full of oil. Also, the shear-stress/ shear-rate curves were obtained by starting at high shear, down to zero, and then back up. SPEJ P. 679^


PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257236
Author(s):  
Zong He Zhu ◽  
Abdul Sami ◽  
Qing Qing Xu ◽  
Ling Ling Wu ◽  
Wen Yin Zheng ◽  
...  

The present study was performed to evaluate the effects of seed priming. This was done by soaking the seeds of two rapeseed cultivars, namely, ZY15 (tolerant to low temperature and drought) and HY49 (sensitive to low temperature and drought), for 12 h in varying solutions: distilled water, 138 mg/L salicylic acid (SA), 300 mg/L gibberellic acid (GA), 89.4 mg/L sodium nitroprusside (SNP), 3000 mg/L calcium chloride (CaCl2), and 30 mg/L abscisic acid (ABA). Primed and non-primed seeds were left to germinate at 15°C and -0.15 MPa (T15W15) and at 25°C and 0 MPa (T25W0), respectively. The results showed that SA, GA, SNP, CaCl2, and ABA significantly improved the germination potential (GP), germination rate (GR), germination index (GI), stem fresh weight (SFW), stem dry weight (SDW), root length (RL), stem length (SL), and seed vigor index (SVI) under T15W15. For ZY15 seeds under T25W0, GA, SNP, CaCl2, and ABA priming reduced the average germination time (96% after 5 days) compared to that of the control (88% after 5 days). For ZY15 seeds under T15W15, SA, SNP, CaCl2, and ABA priming, with respect to the control and water-treated groups, shortened the average germination time (92% after 5 days) compared to that of the control (80% after 5 days). For HY49 seeds under T25W0, GA, SNP, CaCl2, and ABA priming reduced the average germination time (92% after 5 days) compared to that of the control (85% after 5 days). Similarly, for HY49 seeds under T15W15, GA priming shortened the average germination time (89% after 5 days) compared to that of the control (83% after 5 days). These priming agents increased the net photosynthesis, stomatal conductivity, and transpiration rate of rape seedlings under conditions of low temperature and drought stress, while also decreasing intercellular carbon dioxide (CO2) concentrations. Additionally, SA, GA, SNP, CaCl2, and ABA increased superoxide dismutase concentrations (SOD) and ascorbic peroxidase (APX) activities of rape seedlings under stress conditions, while decreasing catalase (CAT) and peroxidase (POD) activities in ZY15 seedlings. In HY49, which is sensitive to low temperature and drought, all priming solutions, except for SNP, led to an increase in SOD activity levels and a decrease in CAT activity levels. Overall, SA, GA, SNP, and CaCl2 increased the concentrations of indoleacetic acid (IAA), GA, ABA, and cytokinin (CTK) in seedlings under stress conditions. Moreover, compared to SA, CaCl2, and ABA, GA (300 mg/L) and SNP (300 mol/L) showed improved priming effects for ZY15 and HY49 under stress conditions.


2017 ◽  
Vol 4 ◽  
pp. e004 ◽  
Author(s):  
Christine Scoffoni ◽  
Grace John ◽  
Herve Cochard ◽  
Lawren Sack

Replacing ultra-pure water solution with ion solution closer to the composition of natural xylem sap increases stem hydraulic conductance by up to 58%, likely due to changes in electroviscosity in the pit membrane pores. This effect has been proposed to contribute to the control of plant hydraulic and stomatal conductance and potentially to influence on carbon balance during dehydration. However, this effect has never been directly tested for leaf xylem, which constitutes a major bottleneck in the whole plant. We tested for an ion-mediated increase in the hydraulic conductance of the leaf xylem (Kx) for seven species diverse in phylogeny and drought tolerance. Across species, no significant changes in Kx were observed between 0 and 15 mM KCl. We further tested for an effect of ion solution during measurements of Kx vulnerability to dehydration in Quercus agrifolia and found no significant impact. These results for leaf xylem contrast with the often strong ion effect reported for stems, and we suggest several hypotheses to account for the difference, relating to the structure of xylem conduits across vein orders, and the ultrastructure of leaf xylem pores. A negligible ion response in leaves would weaken xylem sap ion-mediated control of plant hydraulic conductance, facilitating modeling of whole plant hydraulic behavior and its influence on productivity.


1974 ◽  
Vol 52 (15) ◽  
pp. 2805-2817 ◽  
Author(s):  
David John Harris ◽  
M. T. Thomas ◽  
Victor Snieckus ◽  
E. Klingsberg

The protonation of the 1,2(4H)-diazepines 2a, 2b, and the 1,2(1H)-diazepine 3a in various acidic media has been studied by u.v. and n.m.r. spectroscopy. Compounds 2a and 3a undergo protonation to give 4a and 7a, respectively, whereas 2b provides the monoprotonated species 4b in dilute acid and the diprotonated form 4c in strongly acidic solution. Spectral characteristics of crystalline 1,2(4H)-diazepinium perchlorates 4a, 4d, 7a, and 7c correlate well with those observed for the corresponding free bases in acidic solutions; 7a-ClO4 and 7c-ClO4 were obtained either from 2a and 2c or from 3a and 3c, respectively. In trifluoroacetic acid-d-D2O, deuterium exchange at C4 and C6 of 2a, 2b, and 3a was observed indicating the presence of small equilibrium concentrations of species 1a, 1c and/or 1d, and 1b under these conditions. Temperature variable n.m.r. spectroscopy provides evidence for ring inversion phenomena for the protonated forms 4a and 7a. In the case of 4a, the activation energy, ΔGc≠ = 10.2 ± 0.2 kcal/mol (Tc = 8 ± 3°) has been estimated. The difference in the activation energy between the free base and the protonated form, ΔG≠ (2a)—ΔG≠ (4a) = 6–7 kcal/mol is attributed to strong repulsive N1—N2 lone pair interaction in 2a in the transition state for the ring inversion process.Under vigorous acidic conditions, the 1, 2(4H)-diazepines 2a–c give pyrazoles (10a–c), pyridines (12a–c), and acetophenone. Using identical conditions, the 1,2(4H)-diazepinium salt, 7a-ClO4 provides pyrazole 11a and pyridine 12a and, in addition, the 1-methylaminopyridinium salt 13a. However, rearrangement also proceeds under very mild conditions (ethanol–water) as shown for 7a-ClO4 and 7c-ClO4 which yield compounds 11a and 12a, and 11c, 12c, and 13c, respectively. The 1, 2(1H)-diazepine 3a gives 11a, 12a, and 13a in ethanol–water solution and exclusively 13a in trifluoroacetic acid. The mechanism of these reactions is discussed in terms of formation of open-chain (15) and diaziridine-type (19) intermediates. Electrocylic mechanisms are eliminated from consideration on the basis of the absence of products 23, 24, and 25 which should have been observed from the reactions of 2b, 2c, and 7c-ClO4 if these pathways were operative.


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