scholarly journals Identification of spring wheat lines by the allelic state of Vrn genes for use in winter wheat breeding for carotenoid content

2020 ◽  
pp. 88-95
Author(s):  
O. Leonov ◽  
Ya. Sharypina ◽  
Z. Usova ◽  
K. Suvorova ◽  
T. Sakhno
Keyword(s):  
Winter Wheat ◽  
Spring Wheat ◽  
Dominant Gene ◽  
Wheat Variety ◽  
Wheat Breeding ◽  
Carotenoid Content ◽  
Heterozygous State ◽  
Dominant Allele ◽  
Vrn Genes ◽  
Wheat Lines

The aim of the research is allelic identifi cation of the genes Vrn A1, Vrn B1, Vrn B3, and Vrn D1 in 18 spring wheat samples and 3 lines obtained from winter-spring cross combinations with high carotenoid grain content for winter wheat breeding program. The content of carotenoid pigments in the grain ranged from 0.20 to 8.3 mg/100 g in the analyzed 143 samples of soft wheat. Samples of spring wheat were identifi ed for high content of carotenoids (more than 4.5 mg/100 g of flour): Volgouralskaya, Kinelskaya 61, Lutescens 540, Lutescens 598, Lutescens 575, Lutescens 516, Kinelskaya 2010, Omskaya 41. According to the studies, the presence of the Vrn-A1 allele established in 4 spring wheat samples (Sibiryachka 4, Frontana, Izolda, Dynastiya). The heterozygous state of the Vrn-A1 gene was determined for the Saratovskaya Zolotistaya variety. The presence of the allele Vrn-B1 was identifi ed in the samples Fora, Leningradka, Izolda, Saratovskaya Zolotistaya, Omskiy Tsirkon, Omskaya 41, Lutescens 540. For the samples Lutescens 516, L224-5 the heterozygous state of the locus Vrn-B1was determined. Analysis of the Vrn-B3 gene confi rmed the presence of the Vrn-B3 allele in all tested samples. Only variety Dynastiya carried a dominant allele. The Vrn-D1 gene was identifi ed in a recessive state in samples Fora, Sibiryachka 4, Novosibirskaya 22, Frontana, Leningradka, Kinelskaya 2010, Kinelskaya 61, Volgouralskaya, Omskaya 41, Lutescens 516, Lutescens 540, Lutescens 598, L224–5. In the variety Omskiy Tsircon gene Vrn-D1 was in a heterozygous state. The use of spring carriers of the trait – Samples Omskaya 41 and Lutescens 540, with one dominant gene Vrn-A1, and Lutescens 516, with the dominant allele of the gene Vrn-A1 and polymorphic in the Vrn B1 gene – were the most promising for the winter wheat breeding in the direction of increasing the carotenoids content in flour. Key words: bread wheat, variety, line, vernalization, carotenoids, genes Vrn A1, Vrn B1, Vrn B3, Vrn D1.

Imidazolinone-Resistant Wheat Acetolactate Synthase In Vivo Response to Imazamox

2005 ◽  
Vol 19 (3) ◽  
pp. 539-548 ◽  
Author(s):  
Curtis R. Rainbolt ◽  
Donald C. Thill ◽  
Robert S. Zemetra ◽  
Dale L. Shaner
Keyword(s):  
Winter Wheat ◽  
Spring Wheat ◽  
Dose Response ◽  
Wheat Cultivar ◽  
Single Gene ◽  
Acetolactate Synthase ◽  
Wheat Cultivars ◽  
Whole Plant ◽  
Wheat Lines

Several experiments were conducted to evaluate the utility of an in vivo acetolactate synthase (ALS) assay for comparing sensitivity to imazamox among imidazolinone-resistant wheat cultivars/lines. Ten single-gene imidazolinone-resistant winter wheat cultivars/lines, one two-gene and four single-gene imidazolinone-resistant spring wheat cultivars/lines, and three pairs of heterozygous and homozygous imidazolinone-resistant winter wheat lines were evaluated in the assay experiments. Additionally, a dose-response assay was conducted to evaluate the tolerance of several imidazolinone-resistant wheat cultivars to imazamox on a whole plant level. The I50value (i.e., the imazamox dose that inhibited ALS activity by 50%) of the winter wheat cultivar ‘Above’ was 54 to 84% higher than the I50values of 99-420, 99-433, and CV-9804. However, based on the results of this study, it is unclear whether genetic background or market class (hard red winter vs. soft white winter) influences the level of ALS inhibition by imazamox. Teal 15A, the two-gene imidazolinone-resistant spring wheat cultivar, had an I50value that was two to three times greater than the I50value of the single-gene imidazolinone-resistant spring wheat cultivars/lines. The heterozygous imidazolinone-resistant wheat lines had I50values that were 69 to 81% less than the I50values of the homozygous lines. In the whole plant dose response, theR50values (i.e., the imazamox dose that reduced biomass by 50%) of the susceptible cultivars Brundage 96 and Conan were 15 to 17 times less than the homozygous single-gene imidazolinone-resistant winter and spring cultivars/lines, whoseR50values were about 1.7 times less than theR50value of the two-gene imidazolinone-resistant spring wheat line, Teal 15A. The results of the in vivo ALS imazamox assays and the whole plant imazamox dose-response assay were similar, indicating that the in vivo assay can be used to accurately and quickly compare resistance between imidazolinone-resistant wheat cultivars/lines.

scholarly journals Features of the formation of productivity signs of the yielding capacity in winter wheat varieties under different growing conditions

2020 ◽  
Vol 27 ◽  
pp. 29-34
Author(s):  
V. V. Bazalii ◽  
I. V. Boichuk ◽  
Ya. O. Lavrynenko ◽  
H. H. Bazalii ◽  
Ye. O. Domaratskyi ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Wheat Variety ◽  
Wheat Breeding ◽  
High Plasticity ◽  
Wheat Varieties ◽  
Comparison Research ◽  
Yield Structure ◽  
Growing Conditions ◽  
High Degree ◽  
Winter Wheat Varieties

Aim. Further improvement and application of modern methods of wheat breeding of different types of development for adaptiveness caused by change in the character of manifestation of limiting environmental factors under conditions of the Southern Ukrainian Steppe. Methods. Field experiment, selection and genetic, calculation and comparison research methods were used. Wheat varieties of different genetic and ecological origin under different growing conditions were examined. Results. Analysis of the parameters of plasticity and stability of the elements of the yield structure in winter wheat varieties under different growing conditions revealed that their variability depends on both genotype and ecological gradients. For instance, by the weight of grain from ears only the varieties Kirena and Yaroslavna were distinguished by a high reaction to changes in growing conditions (bi= 1,215 – 1,635) among the analyzed genotypes, the other varieties Askaniiska, Askaniiska Berehynia, Znakhidka odeska and Klarisa manifested quite high plasticity (bi= 0,454 – 0,816). There was an analogous situation in the formation of the number of grains per ear. It is necessary to highlight an important biological feature of these varieties which formed the highest actual productivity under late sowing conditions and different sowing rates when compared to other varieties and realized its potentialities better. Conclusions.The research determined that every wheat variety of different development types should be considered in the aspect of its reaction to different growing conditions and to the capability of realizing genetic potential in a particular ecological region. The results of estimation of adaptiveness potential determined a high degree of homeostaticity in the winter wheat varieties Askaniiska, Askaniiska Berehynia, Perlyna and the variety of an alternative type Klarisa. Keywords: winter wheat, varieties of alternative types, adaptiveness, plasticity, stability.

Variability of resistance to common bunt of wheat

1999 ◽  
Vol 35 (No. 1) ◽  
pp. 26-29 ◽  
Author(s):  
A. Pospisil ◽  
J. Benada ◽  
I. Polisenská
Keyword(s):  
Winter Wheat ◽  
Spring Wheat ◽  
Wheat Variety ◽  
High Variability ◽  
Common Bunt ◽  
Wheat Varieties ◽  
Tilletia Caries ◽  
Winter Wheat Variety ◽  
Seed Dressing ◽  
Spring Wheat Variety

During 1997–1998, the reaction of the collections of winter and spring wheat varieties to Tilletia caries was determined. In both years the most diseased winter varieties were Simona and Sparta, the least diseased were Samara and Ilona. High variability in the level of infection was observed in some varieties (VIada, Estica, Regina, Vega). No winter wheat variety was completely resistant. The most diseased spring wheat variety was Alexandria in both years, while Grandur showed no infection. High variability in the level of infection was observed in the special trials with cv. Ina and Contra. In trials of the efficacy of seed dressing against T. caries and T. laevis there was variability only in the controls, but not in dressed variants.

Inheritance of vernalization response in three populations of spring wheat

1994 ◽  
Vol 74 (4) ◽  
pp. 753-757 ◽  
Author(s):  
P. E. Jedel
Keyword(s):  
Spring Wheat ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Vernalization Response ◽  
Breeding Programs ◽  
Reciprocal Crosses ◽  
Vrn Genes ◽  
Selection For ◽  
Early Late ◽  
Made In

Vernalization responses are known to differ among spring wheat (Triticum aestivum L.) genotypes. Three crosses were made to determine the inheritance of vernalization response in the spring wheat cultivars Cajeme 71, Yecora 70, Glenlea, Pitic 62 and Neepawa. Segregation analyses of days to anthesis were made of the F2 generation in a growth room (25/15 °C, 16/8 h). Segregation analysis of the F3 generation was made in a summer greenhouse. Reciprocal crosses between Neepawa and Pitic 62 indicated an early/late/transgressively late ratio of 12:3:1 in the F2 generation. The F3 generation results fitted an early/late/transgressively late/segregating ratio of 4:1:1:10. Based on the segregation of transgressively late types from both crosses, it was concluded that the genes for spring habit in Pitic 62 and Neepawa were different and not maternally inherited. The Glenlea/Pitic 62 cross produced one transgressively late segregant in an F2 population of 97 plants. The data fitted an early/late/transgressively late ratio of 60:3:1, indicating that Glenlea may differ from Pitic at three Vrn loci. Therefore, either Glenlea or Pitic 62 may carry two dominant Vrn alleles. The reciprocal crosses between Yecora 70 and Cajeme 71 did not segregate transgressively late types in the F2 generation. Therefore, those cultivars had a Vrn allele in common. Selection for vernalization response might be useful when introducing exotic germplasm into spring wheat breeding programs and in manipulating maturity responses. Key words: Vernalization, spring wheat, Vrn genes

scholarly journals Parent material for breading winter wheat with high disease resistance under environments of forest-steppe of Ukraine

2019 ◽  
Vol 24 ◽  
pp. 63-69
Author(s):  
O. A. Demydov ◽  
H. B. Volohdina ◽  
S. I. Voloshchuk ◽  
O. V. Humeniuk ◽  
V. V. Kyrylenko ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Winter Wheat ◽  
Wheat Variety ◽  
Steppe Zone ◽  
Wheat Breeding ◽  
Parent Material ◽  
Starting Material ◽  
Forest Steppe ◽  
High Quality ◽  
High Yielding Varieties

Aim. Main goals were to study the Bulgarian samples of winter wheat, to identify sources of disease resistance for further breeding work and to develop high-yielding varieties being adapted to the conditions of Forest-steppe. Methods. The experimental part of the work was carried out in 1987-2013 in breeding crop rotations of MIW. 1470 Bulgarian samples were the starting material used. Results. The samples M-61-185, 97 / 58-1, 1769-64, 668-2, 49 / 94-168, 301-44-55, 1990 50, 836 / 87-2 were characterized with stabile high resistance to brown rust in combination with yield on the level of adaptive norm; 3324-1, 1919-50, 77 / 83-71, 2579-30-19, 704 / 90-30, 411 / 92-57, 97 / 58-1, Liliia, Milena – to powdery mildew; 1919-50, Liliia, 836 / 87-2, 6532-5, 49 / 94-168, DM-62-44, 835 / 87-44-38, 97 / 58-1 – to Septoria leaf blotch. Genotypes 759-1, 2579-30-19, 836 / 87-2, DM-62- 44, 1919-50 that combine resistance to group of three diseases with complex of traits: winter-hardiness, large grain, productivity, high quality levels and high-yielding; samples with large grain and high quality – 97 / 58-1, MT-17131-87, 71 / 90-1097, 2514-114 are the most valuable for breeders. Based on 2579–30–19 the new winter wheat variety Hospodynia Myronivska has been developed. Conclusions. Bulgarian samples are valuable as a starting material for winter wheat breeding for disease resistance in Forest-steppe zone of Ukraine. Keywords: winter wheat, Bulgarian sample, disease resistance.

scholarly journals Powdery Mildew Resistance of the Lithuanian Winter Wheat Breeding Material

2009 ◽  
Vol 63 (1-2) ◽  
pp. 37-44
Author(s):  
Žilvinas Liatukas ◽  
Vytautas Ruzgas
Keyword(s):  
Powdery Mildew ◽  
Winter Wheat ◽  
Powdery Mildew Resistance ◽  
Quantitative Resistance ◽  
Wheat Breeding ◽  
Resistance Level ◽  
Mildew Resistance ◽  
Breeding Material ◽  
Progress Curve ◽  
Wheat Lines

Powdery Mildew Resistance of the Lithuanian Winter Wheat Breeding Material At the Lithuanian Institute of Agriculture, during 2004-2006, resistance to powdery mildew of approximately 1,500 winter wheat lines was assessed in check and competitive trial nurseries. Our experimental evidence showed that there were no genotypes with effective resistance singlegenes among the lines tested. Effective powdery mildew resistance from start to end of vegetation season depended on the quantitative resistance level. The most resistant lines were evaluated with a score of 2 and area under the disease progress curve (AUDPC) values ranging between 1.0-5.4. The most susceptible genotypes from the collection nurseries had score 8-9 and AUDPC values ranging between 1350-2220. The correlations between maximal disease severity and AUDPC values were strong (r = 0.79-0.92). Genotypes with AUDPC values up to 10 represented 93 lines or 7% in the check trial nursery and 22 lines or 9% in the competitive trial nursery. Lines evaluated with a score 4-5 and AUDPC value 100-300 dominated in 2004. In the next year the dominant genotypes had resistance scores 3-4 and AUDPC value 50-200. The highest powdery mildew resistance (score 2 and AUDPC value 1.0) was identified for the lines Maverich/Victo, Flair/Lut.9392, Strumok/Lut.9321, Zentos/Lut.97-6, Strumok/Lut.9313, Dirigent/Cortez in 2006.

scholarly journals Development of new winter and spring wheat varieties in Latvia

2012 ◽  
Vol 66 (1-2) ◽  
pp. 48-53
Author(s):  
Vija Strazdiņa
Keyword(s):  
Winter Wheat ◽  
Spring Wheat ◽  
Grain Quality ◽  
Wheat Variety ◽  
The State ◽  
Yield Potential ◽  
Wheat Varieties ◽  
Cereal Breeding ◽  
Moderate Resistance ◽  
Spring Wheat Variety

Development of new winter and spring wheat varieties in Latvia The newest wheat varieties developed at the State Stende Cereal Breeding Institute are winter wheat ‘Fredis’ and spring wheat ‘Uffo‘ and ‘Robijs‘. The winter wheat variety ‘Fredis‘ (‘Donskaya Polukarlikovaya‘/‘Abe‘//‘Lowrin 24‘) was developed from 1986 to 2002. The variety has good winter hardiness (7-9 points), earliness (growing period 212-215 days from 1 January), and average yield is 6.0 t ha -1. Grain quality is suitable mostly for food. The variety was registered in the Latvian Plant Catalogue in 2007 and in the Estonian Plant Catalogue in 2011. In the period from 1992 to 2007, the new spring wheat variety ‘Uffo‘ (Sigma/Eta) was created. Variety ‘Uffo‘ is a mid-early variety, with yield potential 6-8 t ha-1, and moderate lodging resistance (5-7 points). Grain quality is suitable for food and feed. The variety ‘Uffo‘ was included in the Latvian Plant Catalogue in 2008 and in the Estonian Plant Catalogue in 2009. The spring wheat variety ‘Robijs‘, obtained from the crossing combinations Dragon/Anninia//Fasan//Fasan by anther culture, was developed at the State Stende Cereal Breeding Institute in collaboration with the Institute of Biology Latvian University from 2000 to 2007. The variety is a middle-early wheat, and is characteriszed by high yield potential (6-8 t ha-1), moderate resistance to lodging (7 points). It has moderate resistance to powdery mildew (3-5 point) and brown rust (10-25%). The grain quality is suitable for food. The variety was included in the Latvian Plant Catalogue in 2011.

Characteristics of winter bread wheat lines developed with the use of spelt wheat

2019 ◽  
pp. 57-64
Author(s):  
I.P. Diordiieva
Keyword(s):  
Winter Wheat ◽  
Bread Wheat ◽  
High Performance ◽  
Wheat Variety ◽  
Triticum Aestivum L ◽  
Biological Traits ◽  
Study Results ◽  
Remote Hybridization ◽  
Wheat Lines ◽  
Wheat Spike

Aim. To create bread wheat lines with valuable economic and biological traits as initial breeding material by hybridization of Triticum aestivum L. and Triticum spelta L. Results and Discussion. The study was conducted at Uman NUH. Bread wheat accessions were created by remote hybridization and multiple individual selections. Hybrid offspring F2–5 was analyzed for expression of economically valuable characteristics. In the 5th generation (F5), the best specimens were selected and tested in 2012–2018 (F5–F10). Bread winter wheat variety Podolianka was used as a check variety. The systematic method of arranging plots of 10 m2 was used. The plots were arranged in blocks with a density of 400,000 plants/ha in 5 replications. Biometric measurements were carried out on 50 plants selected from each plot in 2 non-adjoining replications. After observations and measurements, grain was threshed, and the yield capacity was determined. The significance of the study results was evaluated as Ye. R. Ermantraut et al. described (2000).The developed accessions differed in expression of economically valuable traits. The plant height varied within 52–112 cm. Middle and short forms were selected. The gluten content varied within 27.5–38.1 %. The lines were grouped according to the spike shape into 5 different morphotypes: speltoids, forms with a typical bread wheat spike, square heads, subcompactoids and compactoids. Short square head accession 1689 and accession 1692 with a typical bread wheat spike were distinguished by several economically valuable features. Conclusions. Remote hybridization of bread winter wheat with spelt enabled developing over 500 bread wheat lines. Forms combining high performance and high grain quality were selected, in particular accession 1689 containing 32.4 % of gluten, 15.8 % of protein and giving a yield of 7.19 t/ha. Hybridization of bread wheat with spelt created variety Artaplot included in the State Register of Plant Varieties suitable for dissemination in Ukraine in 2019.

Molecular characterization of vernalization response genes in Canadian spring wheat

Genome ◽  
2007 ◽  
Vol 50 (5) ◽  
pp. 511-516 ◽  
Author(s):  
Muhammad Iqbal ◽  
Alireza Navabi ◽  
Rong-Cai Yang ◽  
Donald F. Salmon ◽  
Dean Spaner
Keyword(s):  
Spring Wheat ◽  
Yield Potential ◽  
High Yield ◽  
Western Canada ◽  
Dominant Allele ◽  
Wheat Cultivars ◽  
Vernalization Response ◽  
Substitution Lines ◽  
Deletion Allele ◽  
Vrn Genes

Vernalization response (Vrn) genes play a major role in determining the flowering/maturity times of spring-sown wheat. We characterized a representative set of 40 western Canadian adapted spring wheat cultivars/lines for 3 Vrn loci. The 40 genotypes were screened, along with 4 genotypes of known Vrn genes, using previously published genome-specific polymerase chain reaction primers designed for detecting the presence or absence of dominant or recessive alleles of the major Vrn loci: Vrn-A1, Vrn-B1, and Vrn-D1. The dominant promoter duplication allele Vrn-A1a was present in 34 of 40 cultivars/lines, whereas the promoter deletion allele Vrn-A1b was present in only 1 of the western Canadian cultivars ( Triticum aestivum L. ‘Rescue’) and 2 of its derivative chromosomal substitution lines. The intron deletion allele Vrn-A1c was not present in any line tested. Only 4 of the western Canadian spring wheat cultivars tested here carry the recessive vrn-A1 allele. The dominant allele of Vrn-B1 was detected in 20 cultivars/lines. Fourteen cultivars/lines had dominant alleles of Vrn-A1a and Vrn-B1 in combination. All cultivars/lines carried the recessive allele for Vrn-D1. The predominance of the dominant allele Vrn-A1a in Canadian spring wheat appears to be due to the allele's vernalization insensitivity, which confers earliness under nonvernalizing growing conditions. Wheat breeders in western Canada have incorporated the Vrn-A1a allele into spring wheats mainly by selecting for early genotypes for a short growing season, thereby avoiding early and late season frosts. For the development of early maturing cultivars with high yield potential, different combinations of Vrn alleles may be incorporated into spring wheat breeding programs in western Canada.

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