scholarly journals Presence of Coliforms and Fecal Coliforms in Fast Food Items of Local Restaurants and Fast Food Outlets of Dhaka City

2012 ◽  
Vol 28 (1) ◽  
pp. 49-51 ◽  
Author(s):  
Mohammad Omar Faruk ◽  
Marufa Zerin Akhter
Keyword(s):  
Middle Class ◽  
Fast Food ◽  
Elevated Temperatures ◽  
Total Coliform ◽  
Food Samples ◽  
Fecal Coliforms ◽  
Human Consumption ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Food Items

The Microbiological status of 50 fast food samples collected from middle class and lower middle class retail outlets of fast food shops and restaurants at New Market and Dhanmondi area of Dhaka city were assessed. Eighty four percent of the samples were found to be satisfactory and 16% of the samples were found to be unsafe for human consumption. Total coliform, fecal coliform, Salmonella spp, Staphylococcus aureus and    fungal count in 12%, 8%, 16%, 14% and 14% samples respectively have crossed the recommended limits and were unsafe for human consumption. Bare-handed handling of foods and use of unsafe water for dish  washing and hand washing by food handlers, keeping food for long time under lighting in display glass boxes with elevated temperatures and storage of uncooked and half cooked meat items side by side in the refrigerator    were noticed to be the possible sources and causes of microbiological contamination of the fast food items. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11810 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 49-51    

Assessment of sensory and microbiological quality of five marketed fish species at Dhaka city in Bangladesh

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 86-92
Author(s):  
T. Islam ◽  
M.R. Mia ◽  
E.A. Folorunso ◽  
M.P. Kabiraz ◽  
M.A. Masuda ◽  
...  
Keyword(s):  
Fish Species ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Plate Count ◽  
Human Consumption ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Retail Markets ◽  
In Situ Conditions ◽  
Significant Difference

The study assessed the sensory quality and the bacterial condition in five nutritionally important fish species at Dhaka city in Bangladesh. Sensory defect points (DP) were analysed at in-situ conditions and microbiological attributes were conducted at laboratory condition. Sensory DP indicated fishes in retail markets (RM) were deteriorating and/or spoiled (DP >3 to <5) except live fishes. In super shops (SS), DP indicated excellent to good quality (DP >1 to <3) for all the fishes. The average total plate count (TPC) in the fishes from RM exceeds the permissible limit (PL) except for the fishes sold in live condition. A highly significant difference observed in the fishes sold in dead condition between RM and SS (p≤0.001). Correlation analysis revealed an insignificant relationship between sensory DP and TPC (p≥0.05) in the markets. The study recorded total coliform counts (TCC) was within the PL i.e., <100 MPN/g in both types of markets. Faecal coliform counts (FCC) were within the acceptable limit i.e., <10 MPN/g only in the fishes from super shops. Salmonella spp. and Vibrio cholerae was detected almost in all samples from retail markets while it was very less likely detected in SS. The study revealed that fish from both RM and SS is safe for human consumption but super shops possess better quality than retail markets.

scholarly journals Occurrence and Antimicrobial Susceptibility Profile of Salmonella Isolates from Animal Origin Food Items in Selected Areas of Arsi Zone, Southeastern Ethiopia, 2018/19

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Minda Asfaw Geresu ◽  
Behailu Assefa Wayuo ◽  
Gezahegne Mamo Kassa
Keyword(s):  
Antimicrobial Susceptibility ◽  
Control Strategies ◽  
Raw Milk ◽  
Food Samples ◽  
Animal Origin ◽  
Salmonella Spp ◽  
Food Items ◽  
Antimicrobial Susceptibility Profile ◽  
Arsi Zone ◽  
Animal Origin Food

The status of Salmonella and its antimicrobial susceptibility profile in animal origin food items from different catering establishments in Ethiopia is scarce. Hence, this study aimed to investigate the occurrence and antimicrobial susceptibility profile of Salmonella isolates from animal origin food items in the selected areas of Arsi Zone. One hundred ninety-two animal origin food samples were collected and processed for Salmonella isolation. Isolates were tested for their susceptibility to 13 antimicrobials using Kirby–Bauer disk diffusion assay. An overall prevalence of 9.4% (18/192) Salmonella spp. isolates were recovered from animal origin food samples collected from different catering establishments. Seven (21.9%) of “Dulet,” 4 (12.5%) of “Kitfo,” 3 (9.4%) of “Kurt,” 2 (6.3%) of raw milk, 1 (3.1%) of egg sandwich and 1 (3.1%) of cream cake samples were positive for Salmonella. Catering establishments, protective clothing, source of contamination, manner of hand washing, and money handling were among the putative risk factors that were significantly associated ( p < 0.05 ) with Salmonella spp. occurrence. Ampicillin, nitrofurans, and sulphonamide resistance were significantly associated ( p < 0.05 ) with Salmonella spp. occurrence in the selected food items. Three (16.7%), 5 (27.8%), 5 (27.8%), and 4 (22.2%) of the isolates were resistant to 3, 4, 5, and 6 antibiotics, respectively, whereas only a sole isolate was resistant to two antibiotics (viz. ampicillin and kanamycin). In conclusion, the general sanitary condition of the catering establishments, utensils used, and personnel hygienic practices were not to the recommended standards in the current study. Besides, detection of multidrug-resistant strains of Salmonella in animal origin food items from different catering establishments suggests the need for detailed epidemiological and molecular characterization of the pathogen so as to establish the sources of acquisition of resistant Salmonella strains. Hence, implementation of Salmonella prevention and control strategies from farm production to consumption of animal origin food items are crucial.

scholarly journals Hygiene Practices and Microbial Contamination of Street-vended Foods in Kenyatta University’s Environs

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Lisa Were ◽  
Gertrude Were ◽  
Kevin Omondi Aduol
Keyword(s):  
Microbial Contamination ◽  
Total Coliform ◽  
Food Samples ◽  
Personal Hygiene ◽  
Human Consumption ◽  
Cross Sectional ◽  
Hygiene Practices ◽  
Microbiological Methods ◽  
And Training ◽  
Main Entrance

Street-vended foods are a major threat to public health because of their microbial contamination. This study investigated hygiene practices and microbial contamination of street foods in Kenyatta University’s environs. Both cross-sectional and experimental designs were adopted. Four (4) major vending stalls at the main entrance to Kenyatta University, gate (A) and at the hind gate at KM shopping center were identified for this study. Twelve (12) food samples were collected from these stalls; sausages, samosas and kachumbari. The foods were collected and transported in cooler boxes to the Microbiology Laboratory at Kenyatta University within 3 hours for analyses. Standard microbiological methods were used for enumeration of Salmonella, coliforms and Escherichia coli. No Salmonella was detected per 25g in all food samples tested. Fifty percent (50%) of kachumbari samples tested positive for E.coli whereas samosas and sausages tested negative. Kachumbari, from all vending stalls, had total coliform levels 4.12 log10 cfu/g, 4.26 log10 cfu/g and 4.21 log10 cfu/g, that did not meet the quality standards (4.00 log10 cfu/g) for ready-to-eat foods. Total coliform counts were below detection limits in samosas and sausages. All (100%) the stalls were exposed to potential contaminants: 75% of the vendors did not wear protective clothing, they handled money and sold food simultaneously, and polythene bags exposed to open air, were used for packaging take away rations. All the foods evaluated were safe for human consumption except kachumbari. Policies on safe street food to be enforced and education and training of vendors on environmental and personal hygiene to be strengthened.

scholarly journals Isolation and quantification of indicator and pathogenic microorganisms along with their drug resistance traits from bottled and jar water samples within Dhaka city, Bangladesh

2020 ◽  
Vol 9 (1) ◽  
pp. 12-14
Author(s):  
Md Aftab Uddin ◽  
Mst Aysha Siddiqua ◽  
Mst Sadia Ahmed
Keyword(s):  
Water Samples ◽  
Bacterial Count ◽  
Fecal Coliforms ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Potential Threat ◽  
Antibiotic Susceptibility Pattern ◽  
Shigella Spp

Commercial drinking water may serve as potential threat to public health if these items are contaminated with a number of pathogenic microorganisms due to faulty manufacturing process. Present study attempted to isolate and quantify the microorganisms from various jar and bottle water samples collected from various areas of Dhaka city. Antibiotic susceptibility pattern of suspected bacterial isolates were also determined in this study. Out of the eighteen samples studied, ten were jar water samples and eight were bottled water samples. The range of total viable bacterial count (TVBC) in these samples ranged from 102 to 105 cfu/ml. Specific pathogens such as, Salmonella spp., Shigella spp., Vibrio spp. and fecal coliforms could not be found in these samples. However coliforms could be detected in 10 samples. The antibiogram study showed that all Escherichia coli and Klebsiella spp. isolates found from these samples were sensitive against gentamicin (10 μg) and azithromycin (30 μg). Variable antibiotic resistance among these bacterial isolates was detected against cefotaxime (30 μg), streptomycin (10 μg) and erythromycin (15 μg). Stamford Journal of Microbiology, Vol.9(1) 2019: 12-14

scholarly journals Microbiological Assessment of Raw Meat Used in Fast-Food Products Sold for Public Consumption

2018 ◽  
Vol 75 (1) ◽  
pp. 78
Author(s):  
Gheorghe ILE ◽  
Alexandra TABARAN ◽  
Sorin Daniel DAN ◽  
Romolica MIHAIU ◽  
Oana REGET ◽  
...  
Keyword(s):  
Fast Food ◽  
Meat Products ◽  
Similar Rate ◽  
Food Samples ◽  
Coliform Bacteria ◽  
Salmonella Spp ◽  
Raw Meat ◽  
Beef Meat ◽  
Public Consumption ◽  
Fried Foods

Raw meat contains sufficient nutrients to support microbial growth and because of that the proper surveillance of the meat products destined for public consumption is mandatory. The purpose of this study was to conduct a microbiological risk assessment regarding the safety of pork, chicken and beef meat products found in fast –food units from Transylvania area. One hundred and seventy samples of raw meat(n=35) and fried foods made of pork(n=39), chicken(n=45)and beef products(n=51) were purchased randomly from 5 different fast-food units located in the study area.The determination performed in order to assess the safety was the total number of Enterobacteriaceae, evaluation of toxigenic E. coli and Salmonella spp. presence, which is also the parameter requested by the current regulation. The levels of contamination with Enterobacteriaceae that exceeded the current legislation limit were 6/39 (15%), 6/45 (13%) and 5/51 (9%) in cooked pork, chicken and respectively beef meat. Given the similar rate of contaminations among these three categories of food there were no statistical differences noticed (p>0.05). Results showed that in 35 of 170 (20%) collected food samples were found total Enterobacteriaceae count over 3 log CFU/g and 12 of 170 (7%) were found with coliform bacteria. Raw meat destined for fast-food industry can represent a risk for public health if hygiene and refrigerating conditions are not properly monitored.

scholarly journals Evaluation of Pathogenic Bacteria Associated with Fresh Produce obtained from Selected Markets in Abeokuta

2013 ◽  
Vol 4 (1) ◽  
pp. 75-81
Author(s):  
OR Afolabi ◽  
AR Oloyede ◽  
TA Ibrahim
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Fresh Produce ◽  
Enterobacter Aerogenes ◽  
Total Coliform ◽  
Shigella Dysenteriae ◽  
Human Consumption ◽  
Salmonella Spp ◽  
Fresh Vegetables ◽  
Plate Counts

The bacterial quality of eight types of fresh produce obtained from selected markets in Abeokuta was determined. Two hundred forty (240) samples of fresh vegetables were examined for aerobic plate counts, coliform counts, and presence of Escherichia coli, toxigenic Staphylococcusaureus, Salmonella spp and Listeria spp. The aerobic plate counts ranged from 2.80 log10 cfu/g to 15.60 log10 cfu/g with the inner parts of cut- water melons having the highest value. Total coliform counts ranged from 0.0 to 11.80 log10 cfu/g. Pathogenic bacteria isolated were Escherichia coli,Staphylococcus aureus, Salmonella spp, Listeria spp, Shigella dysenteriae, Klebsiella pneumoniae, Enterobacter aerogenes, Bacillus spp, Pseudomonas aeruginosa and Streptococcus spp. These pathogens were mostly found at the outer leaves/ parts of the vegetables and render unsafe for human consumption. This study shows that the outer parts/ leaves of fresh produce are heavily contaminated with pathogenic bacteria and the fresh produce should be pre- treated thoroughly, so as to reduce the risk of food- borne outbreaks.Keywords · Pathogenic bacteria · Bacterial quality · Coliform counts

scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Prevalence of Salmonella in dressed and cooked broiler meat of different grocery shops, hotels and restaurants in Gazipur and Dhaka city of Bangladesh

2018 ◽  
Vol 2 (2) ◽  
pp. 40-44
Author(s):  
AKM Ziaul Haque ◽  
SM Lutful Kabir ◽  
Ayesha Siddiqua ◽  
AZM Iftikhar Hussain
Keyword(s):  
Fast Food ◽  
Breast Muscle ◽  
Thigh Muscle ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Broiler Meat ◽  
Poultry Products ◽  
Thigh Muscles ◽  
Cooked Meat

The study was conducted to find out the prevalence of Salmonella in dressed and cooked broiler meat in different shops and restaurants in Gazipur and Dhaka city including the sanitary quality. A total of 80 samples of dressed boiler, 88 cooked samples from road side fast food shops and small hotels, and 104 cooked samples from recognized fast food shops and restaurants were subjected to bacteriological isolation and identification. Highest number of Salmonella spp. were 42.5% in road side shops and 16.25% found in super market for dressed meat. Thigh muscle was more prevalent to breast muscle in both market types for dressed muscle. A total of 31 thigh muscles were positive for road side shop and 23 for super market where 26 and 19 breast muscle were positive for road side shop and super market respectively. Among the cooked meat or fried chicken incidence of Salmonella spp. were 23.86% in road side shops and 11.54% found in recognized shops. In case of both market source thigh muscle was highly prevalent in comparison to breast muscle. For cooked meat, a total of 19 thigh muscles were positive for road side fast food shops and small hotels, and 12 for recognized fast food shops and restaurants where 15 and 8 breast muscle were positive for road side fast food shops and recognized fast food shops respectively. Our results highlighted the need of implementing strict hygiene and sanitation standards to reduce the incidence of Salmonella. The prevalence of Salmonella in poultry products can be reduced effectively by identifying and eliminating the sources and contamination sites during slaughter and processing of poultry. Asian Australas. J. Food Saf. Secur. 2018, 2(2), 40-44

scholarly journals Microbiological analysis of raw milk, pasteurized milk and yogurt samples collected from different areas of Dhaka city, Bangladesh

2015 ◽  
Vol 39 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Tasmina Rahman ◽  
Tanzia Akon ◽  
Iftikharun Nessa Sheuli ◽  
Naima Hoque
Keyword(s):  
Listeria Monocytogenes ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Shigella Spp

A total of 300 different milk and yogurt samples were collected from Dhaka city and analyzed for total viable bacterial count (TVBC), total coliform count (TCC) and presence of several pathogens such as Escherichia coli, Salmonella spp., Shigella spp., Vibrio spp. and Listeria monocytogenes. Raw milk samples collected from Moghbazar area showed highest TVBC (4.2 × 106 cfu/ml) and samples from Mohammadpur area showed lowest count (3.5 × 103 cfu/ml). All the pasteurized milk samples showed TVBC of 1.9 × 102 to 2.8 × 103 cfu/ml, and TCC only in one sample Yogurt samples (open) collected from vendor showed TVBC of 9.1 × 103 to 8.2 × 107 cfu/ml, and TCC only in sample collected from Dhanmondi, Dhaka. The packed yogurt samples showed TVBC of 2.2 × 102 to 6.1 × 103 cfu/ml, and TCC only in one sample. All the milk and yogurt samples were found contaminated with E. coli and Shigella-like species. Listeria monocytogenes was not detected in any of the samples studied.Journal of Bangladesh Academy of Sciences, Vol. 39, No. 1, 31-36, 2015

scholarly journals Microbiological Quality Assessment of Marketed Broiler Meat in Different Markets of Dhaka City

2020 ◽  
Vol 7 (2) ◽  
pp. 261-266
Author(s):  
Mst Tasmim Sultana ◽  
Ashrifa Akter Mukta ◽  
Lita Biswas ◽  
Md Masud Rana
Keyword(s):  
Research Work ◽  
Microbiological Quality ◽  
Multidrug Resistant ◽  
Total Coliform ◽  
Viable Count ◽  
Resistant Bacteria ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Broiler Meat ◽  
Meat Samples

The present research work was undertaken to assess the bacterial quality and to know the prevalence of zoonotic bacteria from broiler meat samples sold in Krishi market, Bihari camp market, Agargaon market, Taltola market and SAU (Sher-e-Bangla Agricultural University) mini bazar, Dhaka, Bangladesh from January to May 2018. After processing of samples primary culture was done in nutrient broth and nutrient agar media then pure culture was obtained from different selective media. Total Viable Count (TVC), Total Coliform Count (TCC) and Total Salmonella Count (TSC) in broiler meat of different broiler markets were determined. Mean of TVC, TCC and TSC for the Krishi market, Agargoan market, Taltola market, Bihari camp market and SAU Mini market were 5.67, 4.32, 2.96 log10 CFU/g, 5.88, 4.64, 3.56 log10 CFU/g, 6.10, 4.68, 3.78 log10 CFU/g, 6.68,4.87, 3.84 log10 CFU/g and 5.84, 4.25, 3.13 log10 CFU/g respectively. The prevalence of Escherichia coli and Salmonella spp. Were 74% and 42% respectively. E. coli isolates were showed sensitive to Ciprofloxacin (91.6%), Gentamycin (87.5%), Azithromycin (66.66%), and Tetracycline (58%) and resistant to Penicillin (79.16%) then Amoxycillin (75%), Streptomycin (75%) and Ampicillin (58.3%). Highest resistant pattern was showed by Tetracycline (58%), Streptomycin (72.72%), Penicillin (72.72%) and Amoxycillin (63.63%). Highest resistant pattern showed by Amoxycillin (71.42%) and Penicillin (71.42%). This study revealed that broiler meat sold at some local markets of Dhaka city were contaminated with multiple species of multidrug resistant bacteria which may risk for human health. Res. Agric., Livest. Fish.7(2): 261-266,  August 2020

Sign in / Sign up

Export Citation Format

Share Document