Clinical and laboratory aspects of reactive thrombocytosis

2021 ◽  
Vol 2 (88) ◽  
pp. 49-53
Author(s):  
N.M. Klimkovich ◽  
◽  
T.Yu. Boytsova ◽  
Ye. A Krasnova ◽  
◽  
...  
Keyword(s):  
Light Microscopy ◽  
Inflammatory Diseases ◽  
Morphological Changes ◽  
Reactive Thrombocytosis ◽  
Surgical Interventions

The article deals with the cases of secondary acquired (reactive) thrombocytosis in patients with various diseases. Thrombocytosis was registered against the background of inflammatory diseases of an infectious and non-infectious nature, during surgical interventions. In all patients with reactive thrombocytosis, no morphological changes in platelets were detected by the method of Fonio light microscopy

scholarly journals The State of the Hemomycocirculatory Bed of Adventitia of Varicose Veins of the Small Pelvis in Women with Chronic Inflammatory Diseases of the Internal Genital Organs

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
Natalya Drohomyretska
Keyword(s):  
Inflammatory Diseases ◽  
Varicose Veins ◽  
Morphological Changes ◽  
Complex Structure ◽  
The State ◽  
Reproductive Age ◽  
Ovarian Vein ◽  
Women Of Reproductive Age ◽  
Vein Wall ◽  
Inflammatory Processes

Hemomicrocirculatory system – is a complex structure that reacts in every pathological process even before the clinical period and takes the first blow. The study of microhemocirculation will provide an opportunity to solve the important for practical medicine questions of pathogenesis of many diseases, as for the prevention and treatment of regional disorders of blood circulation.The objective of the research is to study the state of the hemomicrocirculatory bed (HMCB) of adventitia of varicose veins of the small pelvis (VVSP) in women with chronic inflammatory processes of the organs of the small pelvis (CIPOSP).Materials and methods of research. To evaluate the restructuring of the HMCB of adventitia of VVSP, the operating material of 12 women of reproductive age was used. Mainly, there were pieces of the ovarian vein. The study of the HMCB in the vein wall was performed by the non-injecting method of silver impregnation according to V.V. Kupriyanov. To standardize the results, the condition of the HMCB of adventitia of the venous wall in norm was studied in 5 women of reproductive age, who died as a result of various traumas.Results of the research. After the performed studies, the structural-morphological changes of the HMCB of the adventitia of the small pelvis veins were revealed. The dilation of capillaries, postcapillaries, postcapillary venules was observed. The diameter of the vessels of the HMCB of the ovarian vein adventitia was: venule – 94.21 ± 1.38 μM in comparison with the norm – 48.78 ± 1.60 μM (p<0.001); post-capillary venules – 46.76 ± 1.04 μM in comparison with the norm – 28.29 ± 1.1.01 μM (p<0.001); the capillaries were 11.22 ± 0.14 μM in comparison with the norm – 8.24 ± 0.16 μM (p<0.05), arterioles – 29.02 ± 0.76 μM in comparison with the norm – 25.19 ± 1.15 μM (p<0.01). The architectonics of the arterioles is almost unchanged. Lumen of venules is filled with formed elements. The structure of capillaries is polymorphic. The capillary net was localized and concentrated or was formed as a thick planar net, the capillaries were expanded. There were arterio-venulous anastomoses. Endothelial nuclei are shortened. In some preparations, the diameter of the arterioles corresponded to the diameter of the collection venules.  Conclusions:1. The first discovered by us changes in HMCB of adventitia of varicose veins of the small pelvis in women with CIPOSP can be one of the pathogenetic links of the development and progression of the varicose vein itself, which in turn aggravates the course of chronic inflammation.      2. The timely appointment of drugs that improve microcirculation will enable to prevent the development of dystrophic changes in the vein wall, improve the course of chronic inflammatory processes and reduce or completely eliminate the syndrome of “chronic pelvic pain”.

scholarly journals Necrotizing sarcoid granulomatosis with clinical presentations of recurrent acute abdomen. Case report and literature review

2017 ◽  
Vol 89 (11) ◽  
pp. 60-68 ◽  
Author(s):  
V I Vasilyev ◽  
S G Palshina ◽  
B D Chaltsev ◽  
S G Radenska-Lopovok ◽  
T N Safonova
Keyword(s):  
Acute Abdomen ◽  
Clinical Laboratory ◽  
Morphological Changes ◽  
Abdominal Cavity ◽  
High Dose ◽  
Drug Induced ◽  
Serological Tests ◽  
Surgical Interventions ◽  
First Case ◽  
Clinical Presentations

The authors have described the world’s first case of necrotizing sarcoid granulomatosis (NSG) in a 22-year-old woman with the clinical presentations of acute abdomen, which are associated with abdominal lymph nodal infiltration and necrosis, obvious constitutional disturbances (fever, nocturnal sweats, and significant weight loss), high inflammatory activity (anemia, leukocytosis, high erythrocyte sedimentation rates and C-reactive protein levels), the gradual appearance of splenic and hepatic necrotic foci, and infiltration into the lung and lacrimal glands with the development of unilateral uveitis. The patient underwent five surgical interventions, several needle biopsies for recurrent abdominal syndrome, and long-term antibiotic treatment for presumed sepsis, which had caused drug-induced hepatitis. Bacteriological examination of blood, puncture samples, and removed abdominal cavity tissues, serological tests, and immunomorphogical study of biopsy samples and removed tissues yielded negative results for the presence of bacterial, fungal, and tuberculosis infections. NSG was diagnosed on the basis of the systemic nature of the lesion, the presence of granulomas with severe abdominal lymph nodal necrosis and necrotizing granulomatous/lymphocytic vasculitis in the mesentery and removed spleen, as well as the absence of granulomas in the spleen, appendix, and biopsy materials of the liver, colonic mucosa, and parotid gland. Fludarabine therapy was first used in world practice due to the inefficient treatment with high-dose glucocorticoids and cyclophosphamide and to a disease relapse when reducing their doses. The paper gives a detailed review of the literature on the clinical, laboratory, radiological, and morphological manifestations of the disease, which allow the differential diagnosis of NSG with different variants of granulomatous lesions. Based on the 5-year follow-up of the patient and on the analysis of clinical, laboratory, radiological, and morphological changes, the authors uphold the concept that the disease is an independent nosological entity: necrotizing angiitis with sarcoid reactions, rather than the entity of nodular or classic sarcoidosis.

Regeneration from mesenteric arteries in short-term culture

Development ◽  
1966 ◽  
Vol 15 (2) ◽  
pp. 213-221
Author(s):  
J. D. Feldman ◽  
D. L. Gardner
Keyword(s):  
Light Microscopy ◽  
Morphological Changes ◽  
Laboratory Strain ◽  
Functional Change ◽  
Growth Patterns ◽  
Mesenteric Arteries ◽  
Albino Rats ◽  
Short Term ◽  
Lag Period ◽  
Short Term Culture

The investigations which are described here were originally undertaken in order to investigate the pathogenesis of early hypertension at a time when no morphological changes were evident in adrenergic arteries on light microscopy. Evidence of functional change was therefore sought by comparing the growth patterns of cells from hypertensive and normal mesenteric arteries in culture. The study of the cells which migrated from the explants form the basis for this paper. Month-old Wistar laboratory-strain albino rats were used for the experiment. Young rats were deliberately selected, as fat could be more cleanly dissected away from vessel walls. Also: ‘Tissues from old animals are often more difficult to grow than tissues from young animals and exhibit a longer lag period before growth commences’ (Paul, 1965a). Each week, over a period of 14 weeks, hypertension was induced in a group of rats according to the method of Loomis (1946).

Effect of Dextran 70 on the Platelet Distribution of Ex Vivo Thrombi

1979 ◽  
Author(s):  
M. åberg ◽  
A. Rausing ◽  
U. Hedner ◽  
S.-E. Bergent
Keyword(s):  
Light Microscopy ◽  
Factor Viii ◽  
Healthy Volunteers ◽  
Platelet Function ◽  
Small Dose ◽  
Ex Vivo ◽  
Morphological Changes ◽  
Dextran 70 ◽  
Platelet Aggregates

Infusion of dextran 70 impairs the function of factor VIII and increases the lysability of ex vivo thrombi. To find out whether this increased lysability is accompanied by any morphological changes of the thrombi dextran was infused into four healthy volunteers and one patient with von Wiilebrand’s disease. Dextran was also given to four dogs with 51Cr labelled platelets and 1-1abelled fibrinogen. The thrombi were studied in light microscopy and the distribution of isotopes measured. Dextran caused marked changes in the morphology of the thrombi. The platelet aggregates constituting the head were not formed and the platelets were more evenly distributed in the thrombi. The changes were most pronounced 2-4 hours after the infusion. In the patient with von Wiilebrand’s. disease the structure of the thrombus was abnormal already before the infusion of dextran. Even a small dose of dextran given to this patient prevented the formation of a platelet head. When factor VIII concentrate was given the platelet aggregates constituting the head of thrombus again formed. The findings indicate that factor VIII is of importance for the formation and coherence of the platelet aggregates in ex vivo thrombi. Dextran given intravenously reduces platelet aggregabiIity by impairing the function of factor VIII. The alteration of platelet function, accompanied bv profound changes in the morphology of ex vivo thrombi, may explain the increase in lysability which has been previously shown by us.

scholarly journals Update on surgical management of complex macular holes: a review

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mohd-Asyraaf Abdul-Kadir ◽  
Lik Thai Lim
Keyword(s):  
Surgical Treatment ◽  
Morphological Changes ◽  
Internal Limiting Membrane ◽  
Small Subset ◽  
Human Amniotic Membrane ◽  
Surgical Interventions ◽  
Long Term Study ◽  
Macular Holes ◽  
Surgical Options ◽  
Pars Plana

AbstractModern surgical interventions effectively treat macular holes (MHs) more than 90%. Current surgical treatment for MHs is pars plana vitrectomy with epiretinal membrane, internal limiting membrane (ILM) peeling, gas endotamponade, and prone posturing postoperatively. However, a small subset of MHs imposes challenges to surgeons and frustrations on patients. A narrative review was performed on the surgical treatment of challenging MHs including large and extra-large MHs, myopic MHs with or without retinal detachment, and chronic and refractory MHs. There are robust data supporting inverted ILM flap as the first-line treatment for large idiopathic MHs and certain secondary MHs including myopic MHs. In addition, several studies had shown that ILM flap manipulations in combination with surgical adjuncts increase surgical success, especially in difficult MHs. Even in eyes with limited ILM, surgical options included autologous retinal graft, human amniotic membrane, and creation of a distal ILM flap that can assist in MH closure even though the functional outcome may be affected by the MH chronicity. Despite relative success anatomically and visually after each technique, most techniques require a long-term study to analyze their safety profile and to establish any morphological changes of the MH plug in the closed MHs.

scholarly journals Follistatin-Like 1 Attenuation Suppresses Intervertebral Disc Degeneration in Mice through Interacting with TNF-α and Smad Signaling Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Shaoyi Wang ◽  
Jianlu Wei ◽  
Jie Shi ◽  
Qiting He ◽  
Xiaocong Zhou ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Inflammatory Diseases ◽  
Morphological Changes ◽  
Intervertebral Discs ◽  
Inflammatory Factors ◽  
Wild Type ◽  
Tnf Α

Background. Inflammation plays an important role in intervertebral disc degeneration (IDD). The protein follistatin-like 1 (FSTL1) plays a proinflammatory role in a variety of inflammatory diseases. Objectives. The purpose of this study was to investigate whether IDD could be delayed by inhibiting FSTL-1 expression. Methods. We established a puncture-induced IDD model in wild-type and FSTL-1+/- mice and collected intervertebral discs (IVDs) from the mice. Safranin O staining was used to detect cartilage loss of IVD tissue, and HE staining was used to detect morphological changes of IVD tissue. We measured the expression of FSTL-1 and related inflammatory indicators in IVD tissues by immunohistochemical staining, real-time PCR, and Western blotting. Results. In the age-induced model of IDD, the level of FSTL-1 increased with the exacerbation of degeneration. In the puncture-induced IDD model, FSTL-1-knockdown mice showed a reduced degree of degeneration compared with that of wild-type mice. Further experiments showed that FSTL-1 knockdown also significantly reduced the level of related inflammatory factors in IVD. In vitro experiments showed that FSTL-1 knockdown significantly reduced TNF-α-induced inflammation. Specifically, the expression levels of the inflammatory factors COX-2, iNOS, MMP-13, and ADAMTS-5 were reduced. Knockdown of FSTL-1 attenuated inflammation by inhibiting the expression of P-Smad1/5/8, P-Erk1/2, and P-P65. Conclusion. Knockdown of FSTL-1 attenuated inflammation by inhibiting the TNF-α response and Smad pathway activity and ultimately delayed IDD.

scholarly journals Cyclosporine-A Attenuates Retinal Inflammation by Inhibiting HMGB-1 Formation in Rats with Type 2 Diabetes Mellitus

2020 ◽  
Author(s):  
Peng Wang ◽  
Fei Chen ◽  
Xuedong Zhang
Keyword(s):  
Cyclosporine A ◽  
Inflammatory Diseases ◽  
Morphological Changes ◽  
Underlying Mechanism ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Diabetic Retina ◽  
Tnf Α ◽  
Type 2 Diabetic

Abstract Background:Cyclosporine-A has been regarded as an immunoregulatory and anti-inflammatory drug for the treatment of various immune inflammatory diseases. However, the effect of Cyclosporine-A on the retina of type 2 diabetic rats and the underlying mechanism remains to be elucidated. The objective of the present study was to investigate the effect and mechanism of Cyclosporine-A on diabetic retinopathy. Methods:Male Sprague-Dawley rats were established to type 2 diabetic model.After 6 weeks, diabetic rats and normal controls were intravitreally injected with Cs-A (42 ng/2 μL) to the left eye, and 2μL DMSO to the right eye for the control. Another part of normal wild-type rats was subjected to intravitreal injections into the left eyes with 5 μL PBS or HMGB-1 (5 ng/5 μL) or HMGB-1(5 ng/5 μL) plus Cs-A (42 ng/2 μL), respectively. Retinal morphological changes were observed with hematoxylin–eosin staining. Expressions of HMGB-1, IL-1β and TNF-α were detected by immunohistochemistry, ELISA or western blot. Results:Retinal expression levels of IL-1β and TNF-α were upregulated in type 2 diabetic rats and in normal rats with intravitreal injection of HMGB-1, which were attenuated by intravitreal Cs-A. Moreover, Cs-A decreased HMGB-1 expression in diabetic retina and relieved the retinopathy in type 2 diabetic rats. Conclusions:Intravitreal administration of Cs-A showed a protective effect on retina of diabetic rats, possibly by downregulating retinal expressions of IL-1β and TNF-α via the suppression of HMGB-1.

OPTIMIZATION OF TREATMENT OF TUBAL-PERITONEAL INFERTILITY CAUSED BY CHRONIC SALPINGITIS

2021 ◽  
pp. 52-58
Author(s):  
Kalinkina O.B.
Keyword(s):  
Inflammatory Diseases ◽  
Reproductive Function ◽  
Clinical Effectiveness ◽  
Vitro Fertilization ◽  
Surgical Interventions ◽  
Normal Microflora ◽  
Wide Range ◽  
Inflammatory Processes ◽  
Favorable Conditions

The outpatient stage of therapy is the primary and very responsible link in the introduction of patients with chronic forms of VZOMT, since this pathology has a significant impact on such indicators as perinatal, maternal morbidity and mortality. In addition, VZOMT directly affects the development of infertility, ectopic pregnancy, habitual miscarriage, as well as a decrease in the ovarian reserve after unsuccessful attempts of in vitro fertilization, surgical interventions on the appendages of the uterus. To preserve the reproductive function in patients with VZOMT, an important stage is the implementation of a number of therapeutic and rehabilitation measures. The problems of treatment and rehabilitation of patients with VZOMT undoubtedly remain relevant, since cases of relapses and chronization of inflammatory processes significantly worsen the prognosis for a woman's reproductive function. These problems are both social and economic. Taking into account the wide range of infectious agents of VZOMT, it is recommended to use a complex of antibacterial drugs that cover as many varieties of pathogenic and conditionally pathogenic flora as possible. Moreover, an important aspect of the treatment of inflammatory diseases of the female reproductive system is the use of different forms of drugs and ways of their administration. It is rational to combine oral or parenteral with intravaginal. This combination makes it possible to increase the effectiveness of treatment and contributes to the prevention of relapse of the disease. The inclusion of prednisolone – 21 phosphate in the therapy complex also contributes to increasing the effectiveness of therapy, since it creates favorable conditions for the vital activity of normal microflora and unfavorable conditions for conditionally pathogenic microflora. The results obtained in the study indicate a good clinical effectiveness of complex therapy with the inclusion of Elzhina in the treatment regimen for the treatment of fibrotic processes in the fallopian tubes. The use of a combined drug for intravaginal use containing ornidazole-500 mg, prednisolone-3 mg, econazole-150 mg, neomycin 65000ME (Elzhina drug) allows optimizing the management tactics of patients with tubal – peritoneal infertility caused by chronic salpingitis and increasing the fertility rates in this group of patients.

The Conversion of Yolk into Cytoplasm in the Chick Blastoderm as shown by Electron Microscopy

Development ◽  
1958 ◽  
Vol 6 (1) ◽  
pp. 149-161
Author(s):  
Ruth Bellairs
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Embryonic Development ◽  
Light Microscopy ◽  
Morphological Changes ◽  
Primitive Streak ◽  
Single Stage ◽  
Embryonic Cells ◽  
Chick Blastoderm ◽  
Almost All

In almost all embryos yolk becomes converted into cytoplasm. It has not previously been possible to describe in any detail the morphological changes involved in this process; indeed, when the yolk drops contained within embryonic cells are examined by light microscopy they seem to remain in much the same condition until they are suddenly used up. For this reason they have frequently been considered to be nothing but ‘inert, inactive’ stores of food. By using an electron microscope, however, it has been possible to trace some of the morphological changes which take place in the chick when intra-cellular yolk drops are converted into cytoplasm, and to show that these are not confined to a single stage of embryonic development. Moreover, the discovery of mitochondria within the yolk drops suggests that the yolk drops are not ‘inert’. The following stages have been examined: medium and long primitive streak (as defined by Waddington, 1932, and Abercrombie, 1950), head process, head fold, and 10–16 pairs of somites.

Comprehensive non-drug programs in the treatment of acute inflammatory diseases of the maxillofacial region

2021 ◽  
pp. 66-71
Author(s):  
Valentin Sergeevich Rzhevsky
Keyword(s):  
Electrostatic Field ◽  
Inflammatory Diseases ◽  
Local Treatment ◽  
Low Frequency ◽  
Length Of Hospital Stay ◽  
Main Group ◽  
Control Group ◽  
Maxillofacial Region ◽  
Surgical Interventions ◽  
Results Of Treatment

The article presents the results of treatment of patients with acute inflammatory diseases of the maxillofacial region using non-drug methods of treatment. Study material: 60 patients with acute purulent-inflammatory diseases of the maxillofacial region (phlegmons and abscesses), who were divided into 2 groups: the main group — 30 patients who underwent a course of complex application of broadband electromagnetic therapy and low — frequency alternating electrostatic field and the control group — 30 patients who were treated with drug therapy (antibiotics, metronidazole group drugs, antihistamines, multivitamins, detoxification therapy, local treatment of the wound process), which served as the background in the main group. Objective: to develop and scientifically substantiate the complex application of broadband electromagnetic therapy and low-frequency alternating electrostatic field in patients with inflammatory diseases of the maxillofacial region. Results. In a comparative aspect, the dynamics of the indicators of the leukocyte intoxication index (LII) and the main indicators of the morphological picture of the blood before and after treatment in patients with HIA CHLO under the influence of the developed complex was studied. Conclusion. The developed complex, which includes a low-frequency alternating electrostatic field and broadband electromagnetic therapy, contributes to the formation of pronounced anti-inflammatory and detoxifying effects in patients with inflammatory diseases of the maxillofacial region due to a significant reduction in endogenous intoxication, which allows us to recommend it for inclusion in complex therapeutic and rehabilitation programs after surgical interventions to accelerate the time of purulent wound cleansing and reduce the length of hospital stay.

Sign in / Sign up

Export Citation Format

Share Document