scholarly journals Comparison of Intraocular Cytokine Levels of Choroidal Neovascularization Secondary to Different Retinopathies

2021 ◽  
Vol 8 ◽  
Author(s):  
Chenyi Liu ◽  
Shian Zhang ◽  
Xinyi Deng ◽  
Yijing Chen ◽  
Lijun Shen ◽  
...  
Keyword(s):  
Choroidal Neovascularization ◽  
Inflammatory Cytokines ◽  
Control Group ◽  
Vascular Endothelial ◽  
Chemotactic Protein ◽  
Cytokine Levels ◽  
Inducible Protein ◽  
Endothelial Growth Factor ◽  
The Relationship ◽  
Vegf Level

Purpose: To investigate and compare the aqueous concentrations of vascular endothelial growth factor (VEGF) and other inflammatory cytokines in various choroidal neovascularization (CNV) diseases and types.Methods: This observational study included 127 naive eyes with CNV and 43 control eyes with cataracts. Aqueous humor (AH) samples were obtained prior to intravitreal anti-VEGF injection or cataract surgery. Multiple inflammatory cytokines, including VEGF, interleukin (IL) 6, IL-8, IL-10, interferon-inducible protein 10 (IP-10), and monocyte chemotactic protein 1 (MCP-1) levels, were measured using a multiplex bead assay. The angiogenesis index was defined as the ratio of IP-10 to MCP-1. In addition, the relationship among AH cytokine levels, central macular thickness (CMT), and CNV size on optical coherence tomography angiography (OCTA) was evaluated.Results: Except in the myopic CNV group (P = 0.452), the AH concentration of VEGF was significantly higher in all other CNV groups than in the control group (P < 0.05 for all comparisons). IL-8, IL-10, IP-10, and MCP-1 levels (P < 0.05 for all groups) were significantly higher in all CNV diseases except those with neovascular central serous chorioretinopathy. The angiogenesis index was significantly higher in all CNV diseases (P < 0.05 for all comparisons). The VEGF level may be associated with the size of the CNV on OCTA (p = 0.043).Conclusions: The level of intraocular inflammatory cytokines varied among different CNV diseases and CNV types. Therefore, the angiogenesis index may be a more sensitive indicator of angiogenesis.

The Effect of Combination Platelet Rich Fibrin (PRF) with Bone Graft (Carbonate Apatite) to Vascular Endothelial Growth Factor (VEGF) Level: Tissue Engineering Research

2019 ◽  
Vol 829 ◽  
pp. 9-15
Author(s):  
Ina Hendiani ◽  
Agus Susanto ◽  
Zavani Nur Hikmah
Keyword(s):  
Growth Factor ◽  
Treatment Group ◽  
Bone Graft ◽  
Control Group ◽  
Carbonate Apatite ◽  
Vascular Endothelial ◽  
Platelet Rich Fibrin ◽  
The Difference ◽  
Endothelial Growth Factor ◽  
Vegf Level

Platelet Rich Fibrin (PRF) is a platelet that contains growth factor, such Vascular Endothelial Growth Factor (VEGF) that not only have implication in inflammation, stimulate tissue healing but also bone regeneration. The aim of this study was to evaluate the effect of combination PRF with bone graft (carbonate apatite) to VEGF level. This research is an experimental study, with consecutive sampling technique. This research was conducted by selecting 20 subjects (12 male, 8 female) which divided into two groups; 10 subjects in the treatment group were given bone graft and PRF application while another 10 subjects in the control group were given only bone graft without PRF application. The measurement of VEGF level was taken by inserting the paper point no.30 into sulcus before the surgery (D0) and one week after surgery (D7). VEGF level was assessed by ELISA method. All collected data were analyzed by using Wilcoxon test with p <0.05, and Mann-Whitney test with p <0.05. The treatment group, VEGF level at D0 was 3.8 pg/ml and D7 was 8,1 pg/ml. The difference in the level of VEGF was 4.3 pg/ml. The average of the control group at D0 was 4.5 pg/ml and D7 was 5.4 pg/ml, obtained the difference in the level of VEGF 0.9 pg/ml. The Mann Whitney Test analysis showed there was a significant difference between the treatment group and the control group with p-value 0.038. PRF with bone graft application influence the increased level of VEGF.

scholarly journals Pengaruh Spirulina terhadap VEGF dari Trofoblast Tikus Putih Hamil Strain Wistar yang Diinduksi Interleukin 6

2020 ◽  
Vol 9 (2) ◽  
pp. 123
Author(s):  
Harry K Gondo ◽  
Elizabeth Haryanti
Keyword(s):  
Interleukin 6 ◽  
Positive Control ◽  
Control Group ◽  
Vascular Endothelial ◽  
Control Group Design ◽  
Blue Green Algae ◽  
White Rats ◽  
Post Test ◽  
Endothelial Growth Factor ◽  
Vegf Level

Preeclampsia is a pregnancy disorder with hypertension as one of its common symptoms. Preeclampsia in pregnancy is characterized by increasing of proteinuria, blood pressure and also interleukin 6. Spirulina sp is a thread like shaped blue-green algae, similar to chain of cylindrical cells with 1 to 12 μm diameter of cell membrane. The purpose of this research was to know the effect of spirulina on VEGF level in improving of trophoblast function in pregnant white Rats Wistar. The design of the study was laboratory experimental with post-test only control group design. Twenty-five rats with preeclampsia model induced by interleukin 6, were divided into 5 groups. The groups were control group, positive control group (P0) injected by interleukin-6 dose of 5 ng/ 100 gram body weight (BW), group with spirulina dose of 10 mg/100 gram BW (P1), group with spirulina dose of 20 mg/100 gram BW (P2); and group with spirulina dosage 40 mg/100 gram BW (P3). The Vascular endothelial growth factor (VEGF) levels were in experimental groups measured compared to control. The averages of group spirulina were 81.31 (10 mg/100 gram BW), 65.99 (20 mg/100 gram BW), and 49.62 (40 mg/100 gram BW). In comparison with control group (62.70), VEGF level in group administered by spirulina dose of 20 mg/100 gram BW were close to control group.

scholarly journals Determination of vascular endothelial growth factor concentration in serum of patients with varicose veins after conventional phlebectomy and hybrid treatment

2019 ◽  
Vol 85 (2) ◽  
pp. 33-37 ◽  
Author(s):  
F. Adylkhanov ◽  
A. Fursov ◽  
Y. Noso
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Varicose Veins ◽  
Standard Technique ◽  
Control Group ◽  
Vascular Endothelial ◽  
Serum Samples ◽  
Hybrid Treatment ◽  
Endothelial Growth Factor ◽  
Vegf Level

Objective. To study the change of the vascular endothelial growth factor (VEGF) level and the incidence of recurrence of varicose veins after conventional phlebectomy and hybrid treatment. Materials and methods. Conventional phlebectomy was performed according to standard technique. Hybrid treatment consisted of combination of methods, including laser ablation, ultrasound guided foam form sclerotherapy (aethoxysklerol 1-3%), high degree of ligation (HL), total or partial stripping of saphenous vein. The treatment was personalized to every patient. This study included 134 patients, that were divided into two groups, depending on the method of treatment. Serum samples were collected from patients before operation and in 3-12 months after operation. Control group included 20 healthy persons. We analyzed mean concentration and change of the VEGF levels in patients’ serum before and after treatment. Results. Mean levels of VEGF decreased in 3.95 – 5.38 times after the treatment. Also, recurrent cases were estimated. In the case of the recurrence, VEGF levels were higher than in non-recurrent cases. Conclusions. Therefore, results of the study suggest an association between the recurrence of varicose veins and the change of VEGF level.

scholarly journals Dl-3-n-Butylphthalide Ameliorates Diabetic Nephropathy by Ameliorating Excessive Fibrosis and Podocyte Apoptosis

2021 ◽  
Vol 12 ◽  
Author(s):  
Jingyu Xu ◽  
Zonghao Tang ◽  
Youwu He ◽  
Shufang Cai ◽  
Beini Wang ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Renal Fibrosis ◽  
Smooth Muscle Actin ◽  
Renin Angiotensin System ◽  
Therapeutic Drug ◽  
Vascular Endothelial ◽  
Pathological Mechanism ◽  
Cytokine Levels ◽  
Endothelial Growth Factor ◽  
Vegf Level

Diabetic nephropathy (DN) is a common diabetes associated complication. Thus, it is important to understand the pathological mechanism of DN and find the appropriate therapeutic strategy for it. Dl-3-n-Butylphthalide (DL-NBP) has anti-inflammatory and antioxidant effects, and been widely used for the treatment of stroke and cardiovascular diseases. In this study, we selected three different doses (20, 60, and 120 mg⋅kg−1 d−1) of DL-NBP and attempted to elucidate its role and molecular mechanism underlying DN. We found that DL-NBP, especially at the dose of 60 or 120 mg⋅kg−1 d−1, could significantly ameliorate diabetes-induced elevated blood urea nitrogen (BUN) and creatinine level, and alleviate renal fibrosis. Additionally, the elevated expressions of collagen and α-smooth muscle actin (α-SMA) in the kidney from db/db mice were found to be significantly suppressed after DL-NBP treatment. Furthermore, mechanistic studies revealed that DL-NBP inhibits pro-inflammatory cytokine levels, thereby ameliorating the development of renal fibrosis. Moreover, we found that DL-NBP could not only reduce the endoplasmic reticulum stress (ERS), but also suppress activation of the renin-angiotensin system to inhibit vascular endothelial growth factor (VEGF) level, which subsequently reduces the podocyte apoptosis in kidney of db/db mice. In a word, our findings suggest that DL-NBP may be a potential therapeutic drug in the treatment of DN.

Effect of HylocereusPolyrhizus Rind Extract Toward Interleukin-1β, Vascular Endothelial Growth Factor Expression, Endometriosis Implant Area

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
YanuarEka P. ◽  
Hendy Hendarto ◽  
Widjiati .
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Treatment Group ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Vascular Endothelial ◽  
Mice Model ◽  
Endothelial Growth Factor ◽  
Fruit Rind

Retrograde menstruation lead to I Kappa B Kinase (IKK) fosforilation in peritoneum macrophage and cause secretion of proinflammatory cytokine interleukin1β then stimulate endometriosis cell to produce Vascular Endothelial Growth Factor which lead to increasing of endometriosis lession seen as endometriosis implant area. Cytokine secretion was inhibited through prevention of NF-κB activation by dragon red fruit rind extract (Hylocereuspolyrhizus). The aim of this reserach is to know the effect of dragon red fuit rind extract with 0,25; 0,5; and 1 mg/g bodyweight dosage toward IL-1β, VEGF expression and implant area in endometriosis mice model. The design of this experiment was randomized post test only control group design.Endometrios mice model were made in 14 days and split into two group, positive control group and treatment group after two week negative control group and postive control group were given Na-CMC 0,5% solution consequetively, and treatment group were given dragon red fruit extract with different dosage. Signification number for IL-1β is p>0,05, signification number for VEGF is p>0,05, and implant area signification number is p>0,05. Administration of dragon red fruit rind extract can decrease IL-1β, VEGF, and implant area.

Investigation of Targeting Relationship between Micro-Rna-22 and Vegfr3 in Lung Squamous Cell Carcinoma

2020 ◽  
Vol 23 ◽  
Author(s):  
Zheng Dong ◽  
Qing-Hua Xu ◽  
Yuan-Bin Zhu ◽  
Yong-Feng Wang ◽  
Jie Xiong ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Lung Squamous Cell Carcinoma ◽  
Luciferase Reporter ◽  
Control Group ◽  
Vascular Endothelial ◽  
Luciferase Reporter Gene ◽  
Endothelial Growth Factor

Aims : The present study explored the clinical significance of microRNA-22 (miR-22) expression in lung squamous cell carcinoma and to explore the targeting relationship with vascular endothelial growth factor receptor 3 (VEGFR3). Methods: A total of 49 patients with lung squamous cell carcinoma who underwent surgical treatment was selected. The expression of miR-22 was detected by fluorescence quantitative real-time PCR (qPCR), the expression of VEGFR3 was detected by Western blotting assays, and D240 labeled microlymphatic vessels density (MLVD) was detected immunohistochemistry (IHC). Lung squamous cell carcinoma cell line SK-MES-1 was selected and the targeting relationship between miR-22 and VEGFR3 was analyzed by double luciferase reporter gene assay. Western blotting assays were used to detect the expression of vascular endothelial growth factor-D (VEGF-D) and D240 in the blank control group, empty vector transfection group, miR-22 transfection group, miR-22 and VEGFR3 co-transfection group. Results: The expression range of miR-22 in lung squamous cell carcinoma was 0.8-3.5. The expression of miR-22 in lung squamous cell carcinoma was significantly different by tumor maximum diameter, lymph node metastasis, vascular invasion and TNM stage. The expression of miR-22 was linked to survival time. There was a negative correlation between miR-22 and VEGFR3, miR-22 and MLVD. Double luciferase reporter gene assays showed that miR-22 reduced the luciferase activity of pGL3-VEGFR3-WT transfected cells. Compared with the control group, the expression of VEGF-D and D2-40 in the miR-22 transfection group was significantly decreased. However, VEGF-D and D240 in the miR-22 and VEGFR3 cotransfection group reversed the changes. Conclusion: We assumed that the abnormal expression of miR-22 in lung squamous cell carcinoma may be involved in the development and progression of lung squamous cell carcinoma. MiR-22 negatively regulated the target gene VEGFR3 to mediate lymphangiogenesis. The expression of miR-22 may also be linked to the prognosis of the disease.

scholarly journals Topical bromfenac reduces multiple inflammatory cytokines in the aqueous humour of pseudophakic patients

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Takehiro Matsumura ◽  
Kentaro Iwasaki ◽  
Shogo Arimura ◽  
Ryuji Takeda ◽  
Yoshihiro Takamura ◽  
...  
Keyword(s):  
Growth Factor ◽  
Inflammatory Cytokines ◽  
Aqueous Humour ◽  
Ophthalmic Solution ◽  
Vascular Endothelial ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Before And After ◽  
Endothelial Growth Factor ◽  
Single Blind

AbstractIntraocular surgery is associated with increased ocular inflammation. If maintained for a prolonged period after surgery, this inflammation can cause various complications, including subconjunctival fibrosis and bleb scarring. This clinical trial was a prospective, randomised, single-blind, interventional study comparing the efficacy and safety of 0.1% bromfenac sodium ophthalmic solution and 0.02% fluorometholone ophthalmic suspension in the inhibition of multiple inflammatory cytokines in the aqueous humour of 26 patients with pseudophakic eyes who had undergone phacoemulsification and intraocular lens implantation. The patients were randomly assigned to one of the trial drugs, and aqueous humour samples were collected before and after drug administration. Platelet-derived growth factor-AA levels significantly decreased in both drug groups, but they were significantly higher in the fluorometholone group than in the bromfenac group (P = 0.034). Bromfenac also significantly decreased vascular endothelial growth factor level (P = 0.0077), as well as monocyte chemoattractant protein-1 level (P = 0.013), which was elevated for a prolonged period after phacoemulsification. These data suggest that bromfenac is useful to alleviate prolonged microenvironmental alterations in the aqueous humour of pseudophakic eyes.

scholarly journals Angiogenic parameters and the risk factors for thrombosis in polycythemia vera

2018 ◽  
Vol 72 ◽  
pp. 627-633
Author(s):  
Joanna Boinska ◽  
Grażyna Gadomska ◽  
Katarzyna Ziołkowska ◽  
Karolina Woźniak ◽  
Alicja Bartoszewska-Kubiak ◽  
...  
Keyword(s):  
Risk Factors ◽  
Polycythemia Vera ◽  
Myeloproliferative Neoplasms ◽  
White Blood Cells ◽  
Reference Value ◽  
Control Group ◽  
Vascular Endothelial ◽  
Clinical Issue ◽  
Thrombotic Complications ◽  
Endothelial Growth Factor

Aim: The assessment of angiogenic parameters in so-called “liquid tumors”, such as myeloproliferative neoplasms, remains an open clinical issue. The aim of the study is to evaluate the concentration of vascular endothelial growth factor (VEGF-A) and soluble receptors sVEGFR-1 and sVEGFR-2 in relations to risk factors of thrombosis in patients with polycythemia vera (PV). Material/Methods: A total of 45 patients suffering from newly diagnosed PV and 30 healthy volunteers were enrolled into the study. Polycythemia vera was diagnosed according to the WHO (2008) criteria. In the citrated plasma samples VEGF-A, sVEGFR-1 and sVEGFR-2 were measured using ELISA tests. Results: VEGF-A concentration was three-fold higher and sVEGFR-2 significantly lower in PV patients as compared to the control group. VEGF-A concentration was significantly higher in PV patients with JAK2V617F mutation, as compared to patients without this mutation. SVEGFR-1 and sVEGFR-2 concentrations were similar in the analyzed subgroups. In PV patients with an increased number of white blood cells (WBCs), the above upper reference value (≥10 G/l), VEGF-A concentration was two-fold higher than in patients with WBCs number <10 G/l. However, sVEGFR-1 and sVEGFR-2 concentrations did not differ between the analyzed subgroups. Analysis of correlations revealed only one relation between VEGF-A and WBCs number. Conclusions: Increased VEGF-A and decreased sVEGFR-2 concentrations in polycythemia vera patients as compared to the control group indicate an intensification of the process of angiogenesis. A higher concentration of VEGF-A in PV patients with leukocytosis and a positive correlation between WBCs number and VEGF-A reflect the potential role of VEGF-A in the pathogenesis of thrombotic complications in hypercoagulable state in PV patients.

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