scholarly journals Determining Seed Viability During Fruit Maturation to Improve Seed Production and Availability of New Citrus Rootstocks

2021 ◽  
Vol 12 ◽  
Author(s):  
Deived Uilian de Carvalho ◽  
Daniel A. Boakye ◽  
Tim Gast ◽  
Rui Pereira Leite Junior ◽  
Fernando Alferez
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Seed Viability ◽  
Growing Seasons ◽  
Seed Supply ◽  
Germination Potential ◽  
Citrus Groves ◽  
Citrus Rootstock ◽  
Physiological Quality

In recent years, the pressure for replanting and resetting huanglongbing (HLB or citrus greening) affected citrus groves has led to an inadequate seed supply for the most popular rootstock cultivars in the State of Florida, United States. Early fruit harvesting of citrus rootstock source trees might reduce fruit losses and enhance seed availability, especially in HLB-endemic and hurricane susceptible areas, if the physiological quality of the seeds is adequate. The effects of fruit maturity on seed quality and seedling performance of US-802, US-897, and US-942 citrus rootstocks were investigated for two consecutive growing seasons. The study included the evaluation of seed germination and nursery performance of the citrus rootstock seedlings. The germination test was performed in vitro, where seeds were hand-peeled, surface-sterilized and placed in culture tubes containing basal Murashige and Skoog medium. For the emergence test, seeds were sown in seedling trays containing sterilized growing substrate in a greenhouse with controlled-environment conditions. Rootstock fruits from all three varieties harvested in August and September had seeds with higher germination potential, as more than 90% of the seeds generated seedlings. US-942 had more % of emergence than US-802 and US-897, resulting in faster seed germination; in contrast, US-802 had the faster shoot growth rate. Assays on fruit abscission response showed that by August, fruit from all three varieties were responsive to ethylene and abscised, although response varied and was higher in US-942, suggesting the seeds were mature enough. Taken together, our findings indicate that fruits these three rootstocks can be harvested as early as August in contrast to the current procedures without losing germination potential. This will result in an increase in available seeds for nurseries in Florida.

scholarly journals The Effect of Sodium Hypochlorite (NaOCl) and Heat Treatments on Seed Germination of Rice: An Approach to Restore Seed Viability

2021 ◽  
pp. 49-53
Author(s):  
Monoj Sutradhar ◽  
Subhasis Samanta ◽  
Brijesh Kumar Singh ◽  
Md. Nasim Ali ◽  
Nirmal Mandal
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Sodium Hypochlorite ◽  
Seed Viability ◽  
Germination Percentage ◽  
Heat Treated ◽  
Mother Plants ◽  
Breaking Seed Dormancy ◽  
One Year

Dormancy in rice serves as a mechanism of survival by protecting the seed from germinating in the mother plants; however, it becomes a problem in germination during sowing in soil or under in vitro conditions. This study was conducted to determine the effect of heat treatment and sodium hypochlorite (NaOCl) treatment of seeds on dormancy alleviation. The seeds included both freshly harvested seeds and one-year-old stored seeds, which were tested for germination after different types of seed treatments. Both the treatments increased the germination percentage in seeds, however, it was lesser in the case of old seeds. The best results were obtained from 2% NaOCl treatment for 24 hrs in new seeds, i.e. 92.84±0.103 % germination percentage (GP). However, the higher GP in old seeds were obtained from 48 hrs of heat-treated seeds i.e. 82.9±0.509 % GP. The results of the experiment revealed that rice seeds start to lose viability within a year due to seed dormancy, but this can be reversed with proper measures. These methods of breaking seed dormancy can be considered effective to break seed dormancy and improve seed germination in rice.

scholarly journals Tetrazolium: an important test for physiological seed quality evaluation

2019 ◽  
Vol 41 (3) ◽  
pp. 359-366 ◽  
Author(s):  
José de Barros França-Neto ◽  
Francisco Carlos Krzyzanowski
Keyword(s):  
Quality Evaluation ◽  
Seed Quality ◽  
Seed Viability ◽  
Mechanical Damage ◽  
Tetrazolium Salt ◽  
High Quality ◽  
Physiological Quality ◽  
The 19Th Century ◽  
Important Test ◽  
Tetrazolium Test

Abstract: The production of high quality seeds is linked to a good quality control system. In this system, the tetrazolium test plays an important role in the evaluation of the seed physiological quality, not only due to its relative quickness, but also because of the amount of information that the test presents, such as the indexes of viability and vigor, in addition to providing the diagnosis of possible seed quality problems, such as mechanical damage, insect damage, pre-harvest weathering and deterioration during storage. The development of the test had its beginnings at the end of the 19th century, with great evolution in its concepts and methods in the 20th century. For that, there was the dedication of dozens of professionals in several countries, such as Germany, Japan, Russia, Serbia (former Yugoslavia), United States, Argentina and Brazil. The test indirectly measures the respiration processes that occur in the mitochondria of the cells that make up the tissues of the seeds. The reducing reaction of the solution of the tetrazolium salt under the action of dehydrogenase enzymes results in triphenylformazan, which presents a red carmin coloration. By the interpretation of the resulting staining patterns, seed viability, vigor and the main problems affecting seed quality are determined.

scholarly journals Quality assessment of Urochloa (syn. Brachiaria) seeds produced in Cameroon

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Njehoya Clémence-Aggy ◽  
Ntchapda Fidèle ◽  
Kana Jean Raphael ◽  
Etchu Kingsley Agbor ◽  
Sita R. Ghimire
Keyword(s):  
Seed Germination ◽  
Quality Assessment ◽  
Seed Quality ◽  
Seed Viability ◽  
Quality Parameters ◽  
North West ◽  
Grass Seed ◽  
Significant Relationships ◽  
Livestock Farmers

AbstractUrochloa (syn. Brachiaria) is the most popular fodder of livestock farmers in Cameroon for hay and seed productions. Farmers in Cameroon have been producing Brachiaria seeds for decades for own uses and surplus are sold to neighbours, and to traders from Cameroon and neighbouring countries. However, there is no information available about qualities of these seeds. Fifteen Urochloa seeds samples were collected from farmers and/or government stations in five regions (Adamaoua, East, North, North West, and West) and analysed for major seed quality parameters along with seeds of improved Urochloa cultivar Basilisk imported from Brazil as a check. Study showed significant differences among treatments for various seed quality parameters tested (P < 0.0001). The highest thousand grains weight was recorded in Basilisk (5.685 g), followed by W12 (3.555 g), A05 (3.153 g) and N01 (2.655 g). Caryopsis number and caryopsis weight were highest in Basilisk followed by E09, A06, and W12. Of three conditions tested for seed germination, mean germination was the highest in greenhouse (7.39%) where Basilisk had the highest germination (25.5%) followed by N01 (18.50%), A05 (14.50%) and W12 (12.75%). The seed viability ranged from 18% (E09) to 81% (N01), and there were a positive and highly significant relationships between seed germination and viability traits (r = 0.883; P < 0.0001). This study showed a marked difference in seed quality parameters of Urochloa grass seeds produced in Cameroon, and the potential of developing Urochloa grass seed business in the Northern, Adamaoua and Western regions of Cameroon.

scholarly journals Relationship between pod permeability and seed quality in soybean

2014 ◽  
Vol 36 (3) ◽  
pp. 273-281 ◽  
Author(s):  
Carolina Maria Gaspar de Oliveira ◽  
Francisco Carlos Kryzanowski ◽  
Maria Cristina Neves de Oliveira ◽  
José de Barros França-Neto ◽  
Ademir Assis Henning
Keyword(s):  
Seed Quality ◽  
Lignin Content ◽  
Block Design ◽  
Seed Viability ◽  
Soybean Seed ◽  
Seed Vigor ◽  
Development Stages ◽  
Stage Of Development ◽  
Soybean Genotypes ◽  
Physiological Quality

The aim of this study was to evaluate the influence of pod wall permeability on the physiological quality of soybean seed. The cultivars studied were Sant'Ana, FT-2, FT-10, Bossier, Davis and the breeding line F 84-7-30, with a black seed coat. Pods were collected from plants at the R4, R5, R6, R7 and R8 development stages, which composed the treatments in regard to time of harvest. The parameters of permeability and the lignin content of the pods and the seeds within the pods were evaluated. The seeds were collected just after full maturity (R8), and the following tests were performed: germination, electrical conductivity, and tetrazolium, which determined seed viability and vigor. A randomized complete block design in a split-plot in time arrangement was used, with four replications per treatment. The soybean genotypes (six) composed the plots, and the split-plots consisted of the development stages (R4, R5, R6, R7 and R8). In seed evaluation, the same design was used, reducing the number of treatments to three in the split-plots (R6, R7 and R8). Pod permeability varied with the genotype and stage of development; this affected seed vigor, but not the viability of newly-harvested seeds. The pod lignin content did not show any influence on pod permeability.

scholarly journals Impact of Various Sources of Seed Priming on Growth, Yield and Quality of Spinach Local (Spinacia oleracea) in-vitro and in-vivo Condition

2021 ◽  
pp. 1-8
Author(s):  
Bela Joshi ◽  
P. K. Rai ◽  
Vandana Pandey
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Spinacia Oleracea ◽  
Tap Water ◽  
Cost Effective ◽  
Seed Priming ◽  
Quality Parameters ◽  
Growth And Yield ◽  
Significant Difference

Seed germination, following seedling growth are basic but crucial steps in a plant life cycle which includes proper seed germination which is a basic pre-requisite for a better crop growth and yield. The experiment was conducted in Vegetable Science Department under the Defence Institute of Bio-Energy and Research (DIBER), (DRDO), Pithoragarh, Uttarakhand in the year 2020-2021 to standardize the best treatment of seed priming specific to Spinach (Spinacia oleracea). An investigation was conducted both in a laboratory and on the field with the same treatment that was: (T1) Distilled water, (T2) 1%NaCl, (T3) 1%H2O2, (T4) 1%Na2CO3, (T5) Tap water, (T6) 1%DAP, (T7) Control. Seeds were soaked for 16 hrs and then dried to original moisture content for 2 days. It was found that all the priming treatments showed a significant difference with the control and the highest germination %(lab) with 71% was observed in seeds treated with DAP (T6) and the highest crop yield (field) were observed for 1% Na2CO3. Na2CO3 showed the best result in the field as well as in lab conditions based on different quality parameters followed by DAP. This study showed that seed priming treatments help in the enhancement of seed quality parameters, proved cost-effective and the most economical method. The experiment helps to improve the seed quality using different priming treatments which are cost-effective, economic, environment friendly and one of the quickest methods used for seed enhancement.

scholarly journals Germinación de Chiranthodendron pentadactylon Larr. (Sterculiaceae), en respuesta a la escarificación, temperatura y luz

2017 ◽  
pp. 5
Author(s):  
Reyna Osuna-Fernández ◽  
Guillermo Laguna-Hernández ◽  
Alicia Brechú-Franco ◽  
Alma Orozco-Segovia
Keyword(s):  
Seed Germination ◽  
Seed Coat ◽  
White Light ◽  
Maternal Effect ◽  
Seed Quality ◽  
Variable Temperature ◽  
Red Light ◽  
Seed Viability ◽  
Weather Conditions ◽  
Two Populations

Seed quality and seed germination with scarification and non scarification and variable temperature and light were determined in Ch. pentadactylon seeds form two places of Guerrero and Oaxaca. Also seed viability was determined in seeds from Guerrero stored 6 months at 5º C. Seeds showed to be orthodox and dormant due to an impervious seed coat. The seeds from Guerrero had higher weight, length and water content than those from Oaxaca, however the scarified seeds from Oaxaca reached a higher germination percentages. Germination of scarified seeds of the two populations was enhanced by alternated temperatures and inhibited by far red light. Germination under darkness, red light and white light did not differ significantly. In soil some seeds could be dormant under a low ratio red:far red light transmitted by litter. The differences between the two populations might be due to a maternal effect induced by weather conditions in their geographic localities.

scholarly journals PENGARUH APLIKASI PSEUDOMONAS FLUORESCENS P60 TERHADAP MUTU PATOLOGIS, MUTU FISIOLOGIS, DAN PERTUMBUHAN BIBIT PADI IR 64

2013 ◽  
Vol 13 (2) ◽  
pp. 179-190
Author(s):  
Lisa Navitasari ◽  
Loekas Soesanto ◽  
Ahadiyat Yugi Rahayu
Keyword(s):  
Pseudomonas Fluorescens ◽  
Immersion Time ◽  
Seed Quality ◽  
Germination Rate ◽  
Dry Weight ◽  
Distilled Water ◽  
Greenhouse Study ◽  
Detection And Identification ◽  
Physiological Quality

Effect of Pseudomonas fluorescens P60 on pathological and physiological quality and growth of rice IR 64  seedlings. The research objectives were (1) detection and identification of seed-borne pathogens of IR 64 rice, (2) testing Pseudomonas fluorescents P60 in inhibiting the in vitro growth of seed-borne pathogens colonies, (3) testing P. fluorescents P60 for pathological and physiological seed quality, and (4) testing P. fluorescents P60 on the growth of seedlings in the greenhouse. The results showed that some seed-borne pathogens can be found both on farmers’ IR 64 rice and factory’s; they were Aspergillus flavus, Alternaria padwickii, Pseudomonas glumae, and P. syringae. Application of P. flourescens P60 was able to inhibit the in vitrogrowth of colonies of all seed-borne pathogens, except P. syringae.  Related to pathological quality, the effect of P. flourescens P60 on percentage of seed-borne pathogens attack did not significantly different from that of benomil but smaller than distilled water. On the physiological quality of seeds, treatment of P. flourescens P60 has the same effect with benomil and distilled water, with  germination rate was more than 80%. In the greenhouse study,treatment of seed immersion time  in P. flourescens P60 suspension showed that the effect of immersion time as long as15 minutes and 25 minutes on  seedling height, root length, and seedling dry weightdid not significantly different. were. However, 25 minutes immersion time resulted in fresh seedling weight and root dry weight higher than that of 15 minutes immersion time.

scholarly journals Parental Environmental Effects on Seed Quality and Germination Response to Temperature of Andropogon gerardii

Agronomy ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 304
Author(s):  
Bhupinder Singh ◽  
Shardendu K. Singh ◽  
Satyasai Kumar Matcha ◽  
Vijaya Gopal Kakani ◽  
Chathurika Wijewardana ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Germination Rate ◽  
Temperature Response ◽  
In Vitro Study ◽  
Quality Parameters ◽  
Big Bluestem ◽  
Average Percentage ◽  
Response To Temperature

Parental environments (PEs) affect seed quality and might alter the re-establishment of big bluestem grass due to impacts on seed germination. An in vitro study was conducted to quantify the temperature response of seed germination and its interaction with the PE in big bluestem. Seeds developed under eight PEs consisting of a combination of four day/night growth temperatures (GTs) (20/12, 25/17, 30/22, and 35/27 °C) and two CO2 levels (360 and 720 µL L−1) were germinated at eight temperatures (germination temperatures (GRTs)) ranging from 10 to 42.5 °C. Quadratic and modified bilinear regressions best described the cardinal temperatures for the estimated maximum seed germination (MSG) and seed germination rate (SGR), respectively. The average MSG and SGR showed differential responses to the PEs and significantly declined above a 35 °C GRT across the PEs. For the SGR, the minimum and optimum temperatures showed significant differences from other treatments but the opposite response to elevated CO2, while maximum temperatures significantly declined at high (35/27 °C) and low GTs (20/12 °C). Seed quality parameters, individual seed weight, and C and N contents showed a high correlation (R2 > 60) with the average percentage of seed germination and the SGR. Thus, high temperatures for both the PEs (>30/22 °C) and GRTs (>30 °C) could significantly reduce germination, affecting the re-establishment of big bluestem.

scholarly journals Effect of polyethylene glycol 6000 and storage period on seed quality of cocoa (Theobroma cacao L.)

2014 ◽  
Vol 30 (1) ◽  
Author(s):  
Astiti Rahayu ◽  
Triani Hardiyati ◽  
Ponendi Hidayat
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Storage Period ◽  
Seed Storage ◽  
Peg 6000 ◽  
Randomized Design ◽  
Long Time ◽  
Physiological Quality ◽  
And Storage

Increased productivity of cocoa needs high quality of cocoa seeds which are generally provided by certified seed gardens located far from smallholders farm, where seed delivery takes long time and may reduce physiological quality of seeds. One effort to maintain the seed quality is by treatment the seeds with polyethylene glycol6000 (PEG 6000). This study was aimed to study the interaction of PEG 6000 concentration and storage period on cocoa seed quality, and to determine the best concentration of PEG 6000 and storage period to maintain cocoa seed quality. The research was conducted in seed storage room, seed laboratory and green houseof PPPPTK, Cianjur. The experimental design used was a factorial completely randomized design and each combination treatment repeated three times. The first factor was concentration of PEG 6000 of 0%, 20%, 40%, and 60%, and the second factor was the storage period of 3 weeks, 6 weeks, 9 weeks, and 12 weeks. The results showed that concentration of PEG 60% with three weeks storage period was able to minimize the number of moldy seeds. The concentration of PEG 20%, 40%, and 60% were able to prevent seed germination in storage until six weeks. Concentration of PEG 6000 20% was able to maintain moisture content, seed germination, and germination rate.Key words:cocoa seed, storage, recalcitrant, PEG

scholarly journals Bird and Rust Damage and Seed Molds in 30 Sorghum, Sorghum bicolor (L.) Moench., Lines in Mayagüez, Puerto Rico.

1969 ◽  
pp. 99-112
Author(s):  
Claudio Feliciano ◽  
Paul R. Hepperly ◽  
Antonio Sotomayor-Ríos
Keyword(s):  
Puerto Rico ◽  
Seed Germination ◽  
Seed Quality ◽  
Seed Viability ◽  
Curvularia Lunata ◽  
Strong Positive Correlation ◽  
Colletotrichum Graminicola ◽  
Tropical Conditions ◽  
Rust Severity ◽  
Bird Damage

Thirty sorghum lines were evaluated under humid tropical conditions in Mayagüez, Puerto Rico in 1980. In order of importance, bird damage, seed molds, and rust were the major constraints to optimum yields and seed quality. Finches (Fringillidae) mostly Tiaris bicolor and black birds (Icteridae) mostly Quiscalus niger were the most common sorghum eating birds. Seed losses from birds varied from 0 to 97.3% depending on the sorghum line. The mean loss from birds over all lines was 50.7%. Only two sorghum lines, IS 7013 der (0%) and SC0414-12 (ADN 252) (3%) suffered less than 10% seed losses. Over all sorghum lines a strong positive correlation (r = 0.81**) was found between severity of bird damage and the length of the period between 50% flowering and 50% physiological maturity. A low correlation (r = 0.39*) was found between the 125-seed weight and the severity of bird attack. Twentyfive genera of fungi were found on sorghum seeds. Clean seed frequency (seeds without signs of fungi) was highly correlated with seed germination in in vitro tests (r = 0.89**). During delayed harvest, visible mold on seeds increased markedly. Significant losses in seed germination were found at 1 and 2 weeks delayed harvest. Seeds infected with Fusarium moniliforme and Curvularia lunata were 60% lower in viability than noninfected seeds. Phoma spp. and Nigrospora sp. were associated with 20 to 40% reductions in seed viability. Colletotrichum graminicola did not lower seed viability of infected seeds. Sorghum rust severity varied from 0.6 to 46.6% among lines. All but 6 lines showed rust severity of less than 15%. Over all lines there was no significant correlation between rust severity and yield. Six rust susceptible lines (15 to 46.6% rust severity) yielded 63.8% ± 22.6 g/m2, whereas 6 of the most resistant lines (rust severity of less than 3%) yielded 126.6 ± 46.4 g/m2. Based on pest resistance, rapid grain fill and yield, IS 12610 der, IS 12661 der, and SCO(414-12) (ADN 252) appear to be excellent candidates for further testing at Mayagüez. In avoiding seed pests, sorghum lines appeared to benefit from a rapid germination and emergence, a comparatively long vegetative growth period, and a short uniform grain filling period.

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